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WO1996029866A1 - Procede et dispositif de traitement histologique - Google Patents

Procede et dispositif de traitement histologique Download PDF

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Publication number
WO1996029866A1
WO1996029866A1 PCT/US1996/004457 US9604457W WO9629866A1 WO 1996029866 A1 WO1996029866 A1 WO 1996029866A1 US 9604457 W US9604457 W US 9604457W WO 9629866 A1 WO9629866 A1 WO 9629866A1
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WO
WIPO (PCT)
Prior art keywords
fluid
sample chamber
fluids
sample
volume
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1996/004457
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English (en)
Inventor
Gregory M. Fahy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Resuscitation Technologies Inc
Organ Inc
Original Assignee
Life Resuscitation Technologies Inc
Organ Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Resuscitation Technologies Inc, Organ Inc filed Critical Life Resuscitation Technologies Inc
Priority to AU53823/96A priority Critical patent/AU5382396A/en
Publication of WO1996029866A1 publication Critical patent/WO1996029866A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor

Definitions

  • the invention is related to apparatus and methods for histological processing of biological tissue samples.
  • Tissue samples that are to be examined under a microscope are often processed to replace the water or other fluids inside the tissue cells with a second fluid such as ethanol.
  • the second fluid may be replaced with other fluids in a further processing steps.
  • the tissue sample is immersed in a series of water/ethanol fluid baths. Each succeeding water/ethanol fluid bath has a greater concentration of ethanol. Diffusion causes the higher concentration ethanol in the fluid bath to infiltrate the cells while the water leaves the cells.
  • the tissue sample is immersed in a 100% ethanol fluid bath. As a result of the fluid bath processing, the water in the cells of the tissue sample is replaced with ethanol.
  • histological processing as described above may be accomplished manually or by a device which literally moves a tissue sample from one water/ethanol fluid bath to the next, each fluid bath having a different water/ethanol concentration, until the tissue sample is eventually immersed in a 100% ethanol bath.
  • the processing may also be accomplished by a device that superfuses a tissue sample with fluid from a series of reservoirs, each reservoir containing a fluid having a different water/ethanol concentration, until the tissue sample is superfused with 100% ethanol.
  • a typical prior art processing device which superfuses a tissue sample with fluid from a series of reservoirs is the Miles Scientific Tissue Tek III.
  • a prior art histological processing device that superfuses tissue samples with a series of varying concentration fluid baths is represented schematically in Figure 1.
  • a description of the device will be given assuming that the device is using water/ethanol fluids of varying concentrations.
  • a fluid storage cabinet 30 contains the fluids used to superfuse tissue samples and used fluid that is to be disposed of.
  • Fluid reservoirs 32 are located inside the storage cabinet 30. Each reservoir 32 contains a water/ethanol fluid having a certain concentration.
  • Each reservoir 32 is connected to a supply valve 34, which is capable of selecting the fluid from each reservoir 32 in turn.
  • the outlet of the supply valve is connected to a first supply line 36 that is connected to a supply pump 38.
  • the supply pump 38 is also connected to a second supply line 40 which delivers fluid to a sample chamber 42.
  • One or more tissue samples 44 may be mounted inside the sample chamber 42.
  • a waste line 46 is connected to the sample chamber 42 to remove fluid from inside the sample chamber 42.
  • the waste line 46 drains into a waste reservoir 50 located inside the storage cabinet 30.
  • a waste valve 48 may be provided in the waste line 46 to control draining of fluid from the sample chamber 42 into the waste reservoir 50.
  • the supply valve 34, the supply pump 38 and the waste valve 48 may all be computer controlled so that an automated program may accomplish the histological processing of tissue samples 44 in the sample chamber 42.
  • a first water/ethanol fluid having a first concentration is pumped from a supply reservoir 32 into the sample chamber 42 by the supply pump 38.
  • the waste valve 48 remains closed for a period of time until osmosis causes the concentration of the fluid inside the cells of the tissue samples 44 to approach the concentration of the fluid inside the sample chamber 42. Then the waste valve 48 is opened to drain the used fluid into the waste reservoir 50. Next, the waste valve 48 is again closed. A water/ethanol fluid from a second supply reservoir, which has a higher concentration of ethanol, is pumped into the sample chamber 42 by the supply pump 38. The waste valve remains closed for such a period of time that osmosis can again occur, causing the concentration of the fluid inside the cells of the tissue samples 44 to approach the concentration of the fluid in the sample chamber 42. Then the waste valve 48 is opened to drain the used fluid from the sample chamber 42 into the waste reservoir 50. The above described process is repeated until 100% ethanol is pumped into the sample chamber 42. The end result is that the cells of the tissue samples 44 are infused with 100% ethanol.
  • tissue In a typical histological processing method, after the tissue has been infiltrated with 100% ethanol, it is transferred to a 100% xylene bath, and then into a 100% hot paraffin bath, all within the same chamber. The tissue is then retrieved for paraffin solidification in appropriate tissue containers.
  • An exemplary sequence of water/ethanol solution volumes and concentrations used in the device are set forth in the table below. % v/v Ethanol nc. Volume of Vol. Equiv
  • the prior art devices and methods described above have multiple drawbacks.
  • ethanol consumption per procedure may be far higher than necessary. This increases the cost of purchasing ethanol, and the cost of disposing of the used fluid.
  • the fire hazard of the used ethanol is maximized.
  • the consumption of 0% ethanol solution is also maximized.
  • prior art devices are unnecessarily complicated, adding to the cost of the equipment.
  • Each supply reservoir requires a separate withdrawal line.
  • the supply valve must accommodate as many as 6-12 different supply reservoirs.
  • a histological processing technician must inventory, replace, and handle all of the separate supply reservoirs .
  • prior art device are biologically suboptimum.
  • the imposition of fixed steps of ethanol concentrations, as shown in Figure 2 produces osmotic forces that may damage the tissue and that are carried out in an undesirable manner for tissue integrity.
  • the biologically permissible concentration ratio R that can be imposed by adding a high concentration fluid to a previous lower concentration fluid with biological safety is about 4, including the contribution of the isotonic solutes used in addition to the transitional solvent.
  • the initial immersion bath has a concentration far higher than desirable, which can cause tissue damage during the first immersion step.
  • the initial immersion step may have a concentration ratio as high as 40, which is ten times the optimal ratio.
  • the succeeding fluid baths fail to add further concentrations at rates that approach what is permissible, thus unnecessarily prolonging tissue exposure to the solvents and possibly damaging the tissue due to excessive exposure time.
  • the invention is a device and method of performing histological processing of biological tissue samples wherein the tissue samples are immersed in a fluid bath that has a controlled, gradually varying concentration.
  • a first fluid such as fixative
  • the fluid level in the sample chamber is controlled by allowing fluid to escape from the sample chamber through a waste line connected to the sample chamber.
  • the added second fluid is mixed with the first fluid directly in the sample chamber.
  • the concentration of the second fluid gradually increases.
  • the concentration ratio R becomes irrelevant because step changes in concentration are avoided, which produces the equivalent of low acceptable R values at all times. After a period of time, almost all of the first fluid will have escaped from the sample chamber through the waste line, and the fluid in the sample chamber will be almost 100% of the second fluid.
  • a device and method of practicing the invention may also make use of additional processing steps wherein the tissue sample is immersed in a fluid bath of 100% of the second fluid. This may be accomplished using auxiliary supply reservoirs filled with the second fluid. After the process described above is complete, the fluid in the sample chamber may be completely drained. Next, the sample chamber may be partially filled with a volume of the second fluid, such as 100% ethanol, from an auxiliary supply reservoir. This will ensure that the tissue sample is immersed in a fluid bath containing 100% of the second fluid.
  • a device embodying the present invention may include additional fluid reservoirs having additional fluids.
  • the device can repeat the processing steps to replace the second fluid in the tissue sample with a third fluid. These steps can be repeated for a number of different fluids until the tissue sample is infused with the final desired fluid.
  • Figure 1 is a schematic diagram of a prior art histological processing apparatus
  • Figure 2 is a graph showing the concentration of ethanol over time in a sample chamber of a prior art histological processing device
  • FIG. 3 is a schematic diagram of a histological processing device embodying the present invention.
  • Figure 4 is a graph showing the concentration of ethanol over time in a sample chamber of a histological processing device embodying the present invention
  • Figure 5 is a graph showing the concentration of ethanol over time in a sample chamber of a histological processing device embodying the present invention
  • Figure 6 is a graph showing the concentration of ethanol over time in a sample chamber of a histological processing device embodying the present invention.
  • FIG. 7 is a schematic diagram of another histological processing device embodying the present invention. DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS
  • histological processing devices and methods embodying the present invention are discussed with reference to replacing water in the cells of a tissue sample with ethanol. These fluids are used as examples only and are not intended to be limiting. Any fluids could be used in the devices and methods of the present invention. In addition, although only a single processing is described, the histological processing could be repeated a number of times to replace fluid within the cells of a tissue sample with a series of different liquids such as ethanol, xylene, hot paraffin, and other fluids.
  • a schematic diagram of a histological processing device embodying the present invention is shown in Figure 3.
  • the fluid storage cabinet 30 contains a first fluid supply reservoir 52, and a second fluid supply reservoir 54.
  • the first and second fluid supply reservoirs 52, 54 may be arranged as concentric cylinders.
  • the first and second fluid supply reservoirs are connected to a supply valve 34.
  • the supply valve 34 is connected to a supply line 56, which leads to the sample chamber 42.
  • the sample chamber 42 has an area for mounting one or more tissue samples 44.
  • the tissue samples 44 may be mounted inside a wire mesh cage 58, or a similar structure to immobilize the tissue samples 44.
  • a stir table 60 having a stir bar 62 may be mounted on the floor or side wall of the sample chamber 42. Alternatively, a simple stirring rod or blade may be mounted inside the sample chamber 42 on a rotating shaft of a motor. The stir bar helps to facilitate mixing of fluids in the sample chamber.
  • a first waste line 46 is connected to the sample chamber 42 to allow fluids to be drained from the sample chamber 42.
  • the first waste line 46 is connected to a waste valve 64.
  • the waste valve 64 may be replaced with a waste pump to actively pump fluid from the sample chamber 42.
  • a second waste line 66 is connected to the waste valve 64.
  • the second waste line 66 leads to a waste fluid reservoir 50 in the fluid storage cabinet 30.
  • the tissue samples 44 are first placed in the wire mesh cage 58.
  • the sample chamber is filled to a certain level with a first fluid, such as fixative.
  • the first fluid supply reservoir 52 is filled to a certain level with a second fluid, such as ethanol. It may be necessary to also prime the fluid supply line 56 with the second fluid. In this embodiment of the invention, the fluid level in the sample chamber should be at the same height as the fluid level in the first fluid supply reservoir 52.
  • the stir bar 62 is activated, and the supply valve 34 is operated to connect the first fluid supply reservoir 52 with the sample chamber, through the supply line 56. Because the fluid levels are equal, however, little or none of the second fluid in the first fluid supply reservoir 52 will flow into the sample chamber 42.
  • the waste valve 64 (or waste pump) is then operated to allow fluid from within the sample chamber 42 to flow out into the waste fluid reservoir 50 through the first and second waste lines 46, 66. As fluid escapes the sample chamber 42, and the fluid level in the sample chamber 42 drops, fluid from the first fluid supply reservoir 52 will be drawn by gravity into the sample chamber 42. As the fluid level in the sample chamber continues to drop, the concentration of the second fluid will gradually rise in chamber 42. As the fluid level in the sample chamber 42 approaches the bottom, almost all of the fluid in the supply chamber will comprise the second fluid.
  • the device allows the concentration of the second fluid to gradually rise over a period of time. As the mixing and draining occurs, diffusion will cause the mixed fluid in the sample chamber 42 to infiltrate the cells of the tissue samples 44, while water also leaves the tissue samples 44 by osmosis. As a result, a sudden immersion in a high concentration of the second fluid may be prevented to avoid damaging the tissue samples.
  • element 64 will normally be a pump acting at constant speed. When element 64 is a computer-controlled valve, this valve should be put onto an appropriate duty cycle, not simply opened up. Purely passive fluid flow through valve 64 will often be too rapid at first and too slow later, or too rapid at all times.
  • the supply valve 34 and the waste valve 64 can be controlled to vary the flow rates into and out of the sample chamber 42.
  • the valve control can be used to establish a concentration profile like the one shown in Figure 5, wherein the rate of change of the concentration of ethanol (i.e. , the slope of the line) varies at different times.
  • the concentration can be held steady for a period of time, as shown in Figure 6, to allow tissue equilibration to occur.
  • one or more additional processing steps may be performed after all fluid from the sample chamber 42 and the first fluid reservoir 52 has drained into the waste fluid reservoir 50.
  • the waste valve 64 is closed, and the supply valve 34 is operated to allow a portion of the volume of the second fluid in the second fluid supply reservoir 54 to flow into the sample chamber 42.
  • the waste valve 64 is opened and the second fluid is drained into the waste fluid reservoir 50.
  • This additional processing step may be repeated a number of times to ensure that all the fluid in the cells of the tissue samples 44 is the second fluid.
  • a device embodying the invention there is no requirement for multiple fluid supply reservoirs having fluids of different concentrations. This greatly simplifies the apparatus, and the operation of the apparatus.
  • the fluids can flow between the supply reservoirs, the sample chamber and the waste fluid supply reservoir under the influence of gravity. This allows the entire operation to be accomplished by operating two simple valves, thus eliminating the need for any pumps.
  • the volume of the first fluid in the sample chamber, and the volume of the second fluid in the fluid supply reservoir can be varied to obtain desired concentration mixing results.
  • sample chamber 42 and the fluid supply chambers 52 and 54 may be located at different heights, and/or a pump may be installed in the supply line to deliver appropriate amounts of the second fluid into the sample chamber 42.
  • a pump may be installed in the first and/or second waste lines 46, 66 to actively pump fluid from the sample chamber 42 to the waste fluid reservoir 50.
  • a device embodying the present invention may be completely computer controlled by microprocessor 100, connected to the various valves and pumps in the system.
  • the tissue samples 44 may be located in a recessed area 70 at the bottom of the sample chamber 42.
  • the mixing of the first and second fluids may be accomplished in the reservoir 52 by a fluid flow path 73, which may act under the influence of gravity alone to create a specified concentration profile dictated by computer control of valve and/or pump 72.
  • the premixed, constantly varying concentration fluid may then simply flow over the tissue samples 44 and then into the waste fluid supply chamber 50 via the first and second waste fluid lines 46, 66 and the waste valve 64.
  • tissue chamber 42 remains almost empty during gradual concentration elevation and is needed only if flooding of the tissue with large volumes of secondary fluids, such as xylenes and paraffin, is desired.
  • the drain for chamber 42 may be located in recess 70 to maintain liquid volume in 42 as low as possible during gradual concentration elevation in 70.
  • This embodiment may also include an additional fluid supply reservoir 80 filled with a third fluid.
  • the additional fluid supply reservoir may be used to process the tissue samples 44 again to replace the second fluid (for example, nearly 100% ethanol) in the tissue samples 44 with an additional pure fluid of the type found in reservoir 54 to ensure full equilibration and removal of water.
  • the processing steps could be repeated any number of times for additional fluids.
  • the mandatory volume of pure ethanol consumed is 10.8 liters.
  • a device embodying the present invention could accomplish the same histological processing with 4 liters or less of pure ethanol. This represents a savings of 63% in comparison to the prior art.
  • the current invention reduces operating costs involving ethanol by about two- thirds while making the procedure faster, simpler, and biologically superior.
  • Osmotic/diffusional stress is always small, and there is no need to deal with or to drain intermediate solutions.
  • the speed of gradient progress can be programmed simply by changing rates at which the fluids flow through the supply and waste lines, and concentration ramps that include pauses are easily accommodated.
  • a one way valve may be installed that allows air to enter, but which prevents fumes from the fluid supply reservoirs from escaping to the atmosphere.
  • the second fluid may be supplied from closed variable volume flexible plastic bags. This would simplify handling of the fluids, and would prevent escape of fumes from open fluid supply reservoirs.
  • the sample chamber 42 may comprise a variable volume bag that is partially filled with a biologically inert gas. This would prevent fumes from rising out of the sample chamber and would prevent the tissue samples from being exposed to damage from evaporation when the fluid is drained from the sample chamber.
  • this bag may include a "Zip-lok" type sealing provision for introducing and removing the tissues, and may contain inlet and outlet tubes for ready connection to lines 56, 46, etc.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Dans un dispositif de traitement histologique d'échantillons de tissus, les échantillons sont placés dans une chambre renfermant un volume d'un premier fluide. Un volume d'un second fluide est progressivement introduit dans la chambre en même temps que le premier fluide est évacué. L'introduction et l'évacuation des fluides sont régulées de façon à ce que la concentration de fluide dans ladite chambre varie progressivement jusqu'à occupation de presque 100 % du second fluide. Cette diffusion fait varier progressivement la concentration du fluide dans les cellules des échantillons de tissus en même temps que la concentration du fluide environnant.
PCT/US1996/004457 1995-03-31 1996-04-01 Procede et dispositif de traitement histologique Ceased WO1996029866A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU53823/96A AU5382396A (en) 1995-03-31 1996-04-01 Histological processing device and method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41431595A 1995-03-31 1995-03-31
US08/414,315 1995-03-31

Publications (1)

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003054520A1 (fr) * 2001-12-21 2003-07-03 Microm International Gmbh Chambre de traitement pour echantillons histologiques
DE102008039875A1 (de) * 2008-08-27 2010-03-04 Leica Biosystems Nussloch Gmbh Verfahren und Vorrichtung zum Infiltrieren von Gewebeproben mit Paraffin
CN101923018A (zh) * 2010-09-15 2010-12-22 福州大学 一种生物组织脱水机及其应用
DE102009025574A1 (de) * 2009-06-19 2010-12-23 Leica Biosystems Nussloch Gmbh Verfahren zum automatischen Bearbeiten von Gewebeproben in einem Gewebeprozessor
EP2439510A1 (fr) * 2010-10-06 2012-04-11 Klinipath B.V. Procédé et dispositif de préparation de tissu et moule pour prétraiter un matériau en tissu
DE102011002195A1 (de) * 2011-04-20 2012-10-25 Leica Biosystems Nussloch Gmbh Verfahren und Vorrichtung zum Ablösen und/oder Vereinzeln einer histologischen Probe
DE102011002197A1 (de) * 2011-04-20 2012-10-25 Leica Biosystems Nussloch Gmbh Verfahren und Vorrichtung zum Ablösen und/oder Vereinzeln einer histologischen Probe
WO2019020607A1 (fr) * 2017-07-25 2019-01-31 Maersch Thomas Arrangement pour préparer un échantillon de tissu et notamment pour fabriquer un bloc de cire contenant un échantillon de tissu

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE29073E (en) * 1974-04-22 1976-12-14 Light microscopy processing apparatus
US4604964A (en) * 1981-10-20 1986-08-12 Shandon Southern Products Limited Tissue processing apparatus
US5472876A (en) * 1991-07-08 1995-12-05 The American National Red Cross Computer controlled cryoprotectant perfusion apparatus

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE29073E (en) * 1974-04-22 1976-12-14 Light microscopy processing apparatus
US4604964A (en) * 1981-10-20 1986-08-12 Shandon Southern Products Limited Tissue processing apparatus
US5472876A (en) * 1991-07-08 1995-12-05 The American National Red Cross Computer controlled cryoprotectant perfusion apparatus

Cited By (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6902928B2 (en) 2001-12-21 2005-06-07 Microm International Gmbh Treatment compartment for treating histological samples
WO2003054520A1 (fr) * 2001-12-21 2003-07-03 Microm International Gmbh Chambre de traitement pour echantillons histologiques
US8557511B2 (en) 2008-08-27 2013-10-15 Leica Instruments (Singapore) Pte. Ltd. Method and apparatus for infiltrating tissue samples with paraffin
DE102008039875A1 (de) * 2008-08-27 2010-03-04 Leica Biosystems Nussloch Gmbh Verfahren und Vorrichtung zum Infiltrieren von Gewebeproben mit Paraffin
DE102008039875B4 (de) * 2008-08-27 2014-08-14 Leica Instruments (Singapore) Pte. Ltd. Verfahren und Vorrichtung zum Infiltrieren von Gewebeproben mit Paraffin
DE102009025574A1 (de) * 2009-06-19 2010-12-23 Leica Biosystems Nussloch Gmbh Verfahren zum automatischen Bearbeiten von Gewebeproben in einem Gewebeprozessor
CN101923018A (zh) * 2010-09-15 2010-12-22 福州大学 一种生物组织脱水机及其应用
EP2439510A1 (fr) * 2010-10-06 2012-04-11 Klinipath B.V. Procédé et dispositif de préparation de tissu et moule pour prétraiter un matériau en tissu
DE102011002195B4 (de) 2011-04-20 2020-07-02 Leica Biosystems Nussloch Gmbh Verfahren und Vorrichtung zum Ablösen und/oder Vereinzeln einer histologischen Probe
US8757016B2 (en) 2011-04-20 2014-06-24 Leica Biosystems Nussloch Gmbh Method and apparatus for detaching and/or isolating a histological sample
DE102011002197A1 (de) * 2011-04-20 2012-10-25 Leica Biosystems Nussloch Gmbh Verfahren und Vorrichtung zum Ablösen und/oder Vereinzeln einer histologischen Probe
US9377382B2 (en) 2011-04-20 2016-06-28 Leica Biosystems Nussloch Gmbh Method and apparatus for detaching and/or isolating a histological sample
DE102011002197B4 (de) 2011-04-20 2019-03-07 Leica Biosystems Nussloch Gmbh Verfahren und Vorrichtung zum Ablösen und/oder Vereinzeln einer histologischen Probe
DE102011002195A1 (de) * 2011-04-20 2012-10-25 Leica Biosystems Nussloch Gmbh Verfahren und Vorrichtung zum Ablösen und/oder Vereinzeln einer histologischen Probe
WO2019020607A1 (fr) * 2017-07-25 2019-01-31 Maersch Thomas Arrangement pour préparer un échantillon de tissu et notamment pour fabriquer un bloc de cire contenant un échantillon de tissu
KR20200006113A (ko) * 2017-07-25 2020-01-17 라이카 바이오시스템즈 누슬로흐 게엠베하 조직 샘플을 처리하기 위한, 그리고 특히 조직 샘플을 함유하는 왁스 블록을 제조하기 위한 장치
JP2020523597A (ja) * 2017-07-25 2020-08-06 ライカ ビオズュステムス ヌスロッホ ゲーエムベーハー 組織試料を調製する、特に組織試料を含むワックスブロックを作製する方法
KR102320681B1 (ko) 2017-07-25 2021-11-02 라이카 바이오시스템즈 누슬로흐 게엠베하 조직 샘플을 처리하기 위한, 그리고 특히 조직 샘플을 함유하는 왁스 블록을 제조하기 위한 장치
US12055467B2 (en) 2017-07-25 2024-08-06 Leica Biosystems Nussloch Gmbh Device for preparing a tissue sample and particularly for producing a wax block containing a tissue sample

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