[go: up one dir, main page]

WO1996017927A3 - Reporter cell line - Google Patents

Reporter cell line Download PDF

Info

Publication number
WO1996017927A3
WO1996017927A3 PCT/EP1995/004819 EP9504819W WO9617927A3 WO 1996017927 A3 WO1996017927 A3 WO 1996017927A3 EP 9504819 W EP9504819 W EP 9504819W WO 9617927 A3 WO9617927 A3 WO 9617927A3
Authority
WO
WIPO (PCT)
Prior art keywords
nfλb
expression
cell line
gene
gene product
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP1995/004819
Other languages
French (fr)
Other versions
WO1996017927A2 (en
Inventor
Stefan Nilsson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Karo Healthcare AB
Original Assignee
Karo Bio AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Karo Bio AB filed Critical Karo Bio AB
Priority to AU47134/96A priority Critical patent/AU4713496A/en
Publication of WO1996017927A2 publication Critical patent/WO1996017927A2/en
Publication of WO1996017927A3 publication Critical patent/WO1996017927A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides a cell line including a first reporter gene arranged to express an assayable first gene product, expression of the first reporter gene being AP-1 mediated, and a second reporter gene arranged to express a second assayable gene product, expression of the second reporter gene being mediated by NFλB. Thus the present invention provides a convenient one cell line-based assay system which can be used to rapidly test a large number of compounds for potential anti-inflammatory activity. According to a further aspect of the invention there is provided a method of testing a compound for both AP1 and NFλB inhibitory activity, the method comprising providing cells in accordance with the first aspect of the invention, stimulating AP1 and NFλB activity in the cells and contacting the cells with the compound to be tested and monitoring expression of the first and second gene products wherein inhibition of AP1 expression of the first gene product, and inhibition of NFλB mediated expression of the second gene product is indicative that the compound has both AP1 and NFλB-inhibitory activity.
PCT/EP1995/004819 1994-12-05 1995-12-05 Reporter cell line Ceased WO1996017927A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU47134/96A AU4713496A (en) 1994-12-05 1995-12-05 Reporter cell line

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9424497A GB9424497D0 (en) 1994-12-05 1994-12-05 Reporter cell line
GB9424497.7 1994-12-05

Publications (2)

Publication Number Publication Date
WO1996017927A2 WO1996017927A2 (en) 1996-06-13
WO1996017927A3 true WO1996017927A3 (en) 1996-08-29

Family

ID=10765423

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1995/004819 Ceased WO1996017927A2 (en) 1994-12-05 1995-12-05 Reporter cell line

Country Status (3)

Country Link
AU (1) AU4713496A (en)
GB (1) GB9424497D0 (en)
WO (1) WO1996017927A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002541779A (en) * 1999-02-24 2002-12-10 ザ ジュネラル ホスピタル コーポレーション Cloning method of signaling intermediate
AU2002349784A1 (en) * 2001-12-03 2003-06-17 Asahi Kasei Pharma Corporation Nf-kappab activating genes

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992007072A1 (en) * 1990-10-10 1992-04-30 La Jolla Cancer Research Foundation Method of inhibiting transcription utilizing nuclear receptors
WO1994017208A1 (en) * 1993-01-21 1994-08-04 President And Fellows Of Harvard College Methods and diagnostic kits utilizing mammalian stress promoters to determine toxicity of a compound
DE4311835A1 (en) * 1993-04-07 1994-10-13 Boehringer Ingelheim Int Method for inhibiting gene transcription
WO1995006754A1 (en) * 1993-09-01 1995-03-09 The Regents Of The University Of California Methods for screening antiestrogen compounds
WO1995031722A1 (en) * 1994-05-18 1995-11-23 Ligand Pharmaceuticals, Inc. Screening for cytokine modulators

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992007072A1 (en) * 1990-10-10 1992-04-30 La Jolla Cancer Research Foundation Method of inhibiting transcription utilizing nuclear receptors
WO1994017208A1 (en) * 1993-01-21 1994-08-04 President And Fellows Of Harvard College Methods and diagnostic kits utilizing mammalian stress promoters to determine toxicity of a compound
DE4311835A1 (en) * 1993-04-07 1994-10-13 Boehringer Ingelheim Int Method for inhibiting gene transcription
WO1995006754A1 (en) * 1993-09-01 1995-03-09 The Regents Of The University Of California Methods for screening antiestrogen compounds
WO1995031722A1 (en) * 1994-05-18 1995-11-23 Ligand Pharmaceuticals, Inc. Screening for cytokine modulators

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
A. RAY AND K.E. PREFONTAINE: "Physical association and functional antagonism between the p65 subunit of transcription factor NF-kappaB and the glucocorticoid receptor", PROC. NATL.ACAD SCI., vol. 91, January 1994 (1994-01-01), NATL. ACAD SCI.,WASHINGTON,DC,US;, pages 752 - 756, XP002004799 *
ALAM J ET AL: "REPORTER GENES: APPLICATION TO THA STUDY OF MAMMALIAN GENE TRANSCRIPTION", ANALYTICAL BIOCHEMISTRY, vol. 188, no. 2, 1 August 1990 (1990-08-01), pages 245 - 254, XP000563914 *
C. JONAT ET AL.: "Antitumor promotion and antiinflammation: Down-modulation of AP-1(Fos/Jun) activity by glucocorticoid hormone", CELL, vol. 62, 21 September 1990 (1990-09-21), CELL PRESS,CAMBRIDGE,MA,US;, pages 1189 - 1204, XP002004798 *

Also Published As

Publication number Publication date
GB9424497D0 (en) 1995-01-25
WO1996017927A2 (en) 1996-06-13
AU4713496A (en) 1996-06-26

Similar Documents

Publication Publication Date Title
ATE260989T1 (en) INSIGHTS FROM GENOME RESEARCH FOR THE SEARCH FOR NOVEL ACTIVE INGREDIENTS
WO1999039001A3 (en) Nucleic acid analysis method
EP0382433A3 (en) Detection of nucleic acid sequences using fluorescence polarisation
CA2202154A1 (en) Methods for drug screening
AU6837287A (en) Amplified hybridization assay
AU3138784A (en) Nucleic acid probe for detecting micro-organism by hybridization technique
ES8609472A1 (en) Use of non-hybridizable nucleic acids for the detection of nucleic acid hybridization
WO2001061033A3 (en) Multiplex ligatable probe amplification
ES2083955T3 (en) DETERMINATION OF POLYNUCLEOTIDES WITH SELECTABLE SPLIT SITES.
WO2003029480A3 (en) Measurements of multiple molecules using a cryoarray
EP0874913A4 (en) Multiple reporter gene assay
IL71715A0 (en) Method,gene probes and test kit for detecting bacteria by nucleic acid hybridization
EP1323728A3 (en) Method of preparing a standard diagnostic gene transcript pattern
AUPQ422399A0 (en) Method of screening transformed or transfected cells
WO1993020230A1 (en) Electrochemical detection of dna hybridisation
FI953990L (en) A method for rendering a nucleic acid amplification reaction product incapable of being subjected to further amplification, a diagnostic assay method using this method, and a test kit and container suitable for carrying out the method or assay
BR9810844A (en) Process for detecting a microorganism in a test liquid sample, and an assay device for conducting the process
PT1141358E (en) Stably transfected cells for identifying compounds which affect stability of mrna
AUPO128096A0 (en) Biosensors
WO1991014003A3 (en) Characterization and analysis of polymorphic vntr loci
EP0210021A3 (en) Carbonic anhydrase inhibitor-tagged nucleic acid probes
WO1998007844A3 (en) Screening assay for the identification of agents which alter expression of pth-rp
WO1996017927A3 (en) Reporter cell line
WO2000058521A3 (en) Methods for the identification of reporter and target molecules using comprehensive gene expression profiles
EP0228949A3 (en) Test method and apparatus for cellular array processor chip

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU CA JP KR US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: CA