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WO1996041608A3 - Thionine pyrularia contenant des immunotoxines ainsi que des conjugues similaires aux immunotoxines - Google Patents

Thionine pyrularia contenant des immunotoxines ainsi que des conjugues similaires aux immunotoxines Download PDF

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Publication number
WO1996041608A3
WO1996041608A3 PCT/US1996/008811 US9608811W WO9641608A3 WO 1996041608 A3 WO1996041608 A3 WO 1996041608A3 US 9608811 W US9608811 W US 9608811W WO 9641608 A3 WO9641608 A3 WO 9641608A3
Authority
WO
WIPO (PCT)
Prior art keywords
thionin
immunotoxin
human
cells
conjugates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1996/008811
Other languages
English (en)
Other versions
WO1996041608A2 (fr
Inventor
Leo P Vernon
Eppie D Rael
Sardar E Gasanov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
THERA PRO
Original Assignee
THERA PRO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by THERA PRO filed Critical THERA PRO
Priority to AU59817/96A priority Critical patent/AU5981796A/en
Publication of WO1996041608A2 publication Critical patent/WO1996041608A2/fr
Publication of WO1996041608A3 publication Critical patent/WO1996041608A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6801—Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent
    • A61K47/6803—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates
    • A61K47/6811—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates the drug being a protein or peptide, e.g. transferrin or bleomycin
    • A61K47/6817—Toxins
    • A61K47/6819—Plant toxins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6835—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site
    • A61K47/6849—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site the antibody targeting a receptor, a cell surface antigen or a cell surface determinant

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Botany (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Medical Informatics (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La présente invention se rapporte à une immunotoxine comportant un anticorps monoclonal CD5-antihumain de souris conjugué à la toxine thionine Pyrularia (thionine P.). La thionine P. est un petit peptide très stable dont la membrane est active et qui présente une toxicité pour certaines cellules eucaryotes et pour toutes les cellules procaryotes testées jusqu'à présent. L'immunotoxine de thionine P. présente un taux élevé de cytotoxicité et de spécificité. L'immunotoxine de thionine P. s'est révélée cytotoxique pour des cellules T qui expriment l'antigène CD5, mais elle n'a présenté aucune réactivité vis-à-vis des cellules B humaines, des erythrocytes humains ou du mouton ou encore des lymphocytes de la souris qui n'expriment pas l'antigène CD5 humain.
PCT/US1996/008811 1995-06-07 1996-06-05 Thionine pyrularia contenant des immunotoxines ainsi que des conjugues similaires aux immunotoxines Ceased WO1996041608A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU59817/96A AU5981796A (en) 1995-06-07 1996-06-05 Pyrularia thionin containing immunotoxins and immunotoxin-li ke conjugates

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US47979995A 1995-06-07 1995-06-07
US08/479,799 1995-06-07

Publications (2)

Publication Number Publication Date
WO1996041608A2 WO1996041608A2 (fr) 1996-12-27
WO1996041608A3 true WO1996041608A3 (fr) 1997-02-06

Family

ID=23905495

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/008811 Ceased WO1996041608A2 (fr) 1995-06-07 1996-06-05 Thionine pyrularia contenant des immunotoxines ainsi que des conjugues similaires aux immunotoxines

Country Status (2)

Country Link
AU (1) AU5981796A (fr)
WO (1) WO1996041608A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9926875D0 (en) 1999-11-12 2000-01-12 Microbiological Research Agenc Use of lytic toxins and toxin conjugates
AU2000275771B2 (en) * 2000-09-11 2006-06-29 Therapro Technologies, Inc. Thionin as an antineoplastic and immunostimulant
WO2019108863A1 (fr) 2017-11-29 2019-06-06 Magenta Therapeutics, Inc. Compositions et procédés pour la déplétion des cellules cd5+
WO2020023561A1 (fr) * 2018-07-23 2020-01-30 Magenta Therapeutics, Inc. Utilisation d'un conjugué anticorps anti-cd5 -médicament (adc) dans une thérapie cellulaire allogénique
US20230135930A1 (en) 2019-04-24 2023-05-04 Heidelberg Pharma Research Gmbh Amatoxin antibody-drug conjugates and uses thereof

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ARCH. BIOCHEM. BIOPHYS., April 1985, Vol. 238, No. 1, VERNON et al., "A Toxic Thionin from Pyrularia Pubera: Purification, Properties and Amino Acid Sequence", pages 18-29. *
BIOCHIM. BIOPHYS. ACTA, 1994, Vol. 1198, BRINKMANN et al., "Immunotoxins Against Cancers", pages 27-45. *
BLOOD, 01 April 1990, Vol. 75, No. 7, BYERS et al., "Use of an Anti-Pan T-Lymphocyte Ricin A Chain Immunotoxin in Steroid-Resistant Acute Graft-Versus-Host Disease", pages 1426-1432. *
CANCER IMMUNOL. IMMUNOTHER., 1983, Vol. 15, IMAI et al., "Selective in Vitro Toxicity of Purothionin Conjugate to the Monoclonal Antibody 225.28S to a Human High-Molecular-Weight Melanoma-Associated Antigen", pages 206-209. *
CANCER IMMUNOL. IMMUNOTHER., 1993, Vol. 36, PORRO et al., "In Vitro and in Vivo Properties of an Anti-CD5-Momordin Immunotoxin on Normal and Neoplastic T Lymphocytes", pages 346-350. *
J. TOXICOL., 1992, Vol. 11, No. 3, VERNON L.P., "Pyrularia Thionin: Physical Properties, Biological Response and Comparison to Other Thionins and Cardiotoxin", pages 169-191. *

Also Published As

Publication number Publication date
WO1996041608A2 (fr) 1996-12-27
AU5981796A (en) 1997-01-09

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