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WO1995020039B1 - Proteases particularisees a activite de transacylation modifiee - Google Patents

Proteases particularisees a activite de transacylation modifiee

Info

Publication number
WO1995020039B1
WO1995020039B1 PCT/US1995/006682 US9506682W WO9520039B1 WO 1995020039 B1 WO1995020039 B1 WO 1995020039B1 US 9506682 W US9506682 W US 9506682W WO 9520039 B1 WO9520039 B1 WO 9520039B1
Authority
WO
WIPO (PCT)
Prior art keywords
carboxypeptidase
customized
amino acid
preselected
modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1995/006682
Other languages
English (en)
Other versions
WO1995020039A2 (fr
WO1995020039A3 (fr
Filing date
Publication date
Priority claimed from US08/329,892 external-priority patent/US6187579B1/en
Application filed filed Critical
Priority to AU26513/95A priority Critical patent/AU679861B2/en
Priority to JP7517672A priority patent/JPH09504438A/ja
Priority to NZ283995A priority patent/NZ283995A/xx
Priority to EP95921432A priority patent/EP0723585A1/fr
Publication of WO1995020039A2 publication Critical patent/WO1995020039A2/fr
Publication of WO1995020039A3 publication Critical patent/WO1995020039A3/fr
Anticipated expiration legal-status Critical
Publication of WO1995020039B1 publication Critical patent/WO1995020039B1/fr
Ceased legal-status Critical Current

Links

Abstract

L'invention se rapporte à des protéases particularisées (en d'autres termes des enzymes mutantes), par exemple la carboxypeptidase Y, à des procédés de production de ces protéases ainsi qu'à des procédés d'utilisation de ces dernières. Ces protéases particularisées sont dérivées des protéases connues. Lesdites protéases présentent l'aptitude à effectuer des réactions de transacylation modifiées, y compris des réactions de transacylation qui ne sont généralement pas catalysées par les protéases connues ou des réactions de transacylation à rendements améliorés, ou encore les deux types de réactions. Les procédés selon l'invention permettent d'obtenir des protéases particularisées par la mutagénèse dirigée ou aléatoire des aminoacides du site actif des protéases connues. L'invention se rapporte également à des procédés d'utilisation des protéases particularisées pour préparer des produits de transacylation présélectionnés. Les produits de transacylation ainsi obtenus peuvent être modifiés par la substitution, au niveau de l'extrémité N ou C, de nucléophiles tels que des L-aminoacides, des D-aminoacides, des amides d'aminoacide et des aminoacides radioactifs.
PCT/US1995/006682 1993-10-28 1994-10-28 Proteases particularisees a activite de transacylation modifiee Ceased WO1995020039A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU26513/95A AU679861B2 (en) 1993-10-28 1994-10-28 Customized proteases with altered transacylation activity
JP7517672A JPH09504438A (ja) 1993-10-28 1994-10-28 改変したアシル基転移活性を有する特製プロテアーゼ
NZ283995A NZ283995A (en) 1993-10-28 1994-10-28 Customized (mutant) carboxypeptidase Y having modified S1 subsite
EP95921432A EP0723585A1 (fr) 1993-10-28 1994-10-28 Proteases particularisees

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US14470493A 1993-10-28 1993-10-28
US08/144,704 1993-10-28
US08/329,892 US6187579B1 (en) 1993-10-28 1994-10-27 Customized proteases
US08/329,892 1994-10-27

Publications (3)

Publication Number Publication Date
WO1995020039A2 WO1995020039A2 (fr) 1995-07-27
WO1995020039A3 WO1995020039A3 (fr) 1996-03-21
WO1995020039B1 true WO1995020039B1 (fr) 1996-05-30

Family

ID=26842276

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/006682 Ceased WO1995020039A2 (fr) 1993-10-28 1994-10-28 Proteases particularisees a activite de transacylation modifiee

Country Status (7)

Country Link
US (2) US6187579B1 (fr)
EP (1) EP0723585A1 (fr)
JP (1) JPH09504438A (fr)
AU (1) AU679861B2 (fr)
CA (1) CA2174525A1 (fr)
NZ (1) NZ283995A (fr)
WO (1) WO1995020039A2 (fr)

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US6537776B1 (en) * 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
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US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US6740506B2 (en) 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
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US6352842B1 (en) 1995-12-07 2002-03-05 Diversa Corporation Exonucease-mediated gene assembly in directed evolution
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US20040023327A1 (en) * 1995-12-07 2004-02-05 Short Jay M. End selection in directed evolution
US6361974B1 (en) 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6939689B2 (en) 1995-12-07 2005-09-06 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US20030219752A1 (en) * 1995-12-07 2003-11-27 Diversa Corporation Novel antigen binding molecules for therapeutic, diagnostic, prophylactic, enzymatic, industrial, and agricultural applications, and methods for generating and screening thereof
US6713279B1 (en) 1995-12-07 2004-03-30 Diversa Corporation Non-stochastic generation of genetic vaccines and enzymes
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6562594B1 (en) 1999-09-29 2003-05-13 Diversa Corporation Saturation mutagenesis in directed evolution
DE19636035A1 (de) 1996-09-05 1998-03-12 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
WO1998020156A1 (fr) * 1996-11-04 1998-05-14 Merck Frosst Canada & Co. Test de liaison d'hydrolases
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DE19857687A1 (de) 1998-12-15 2000-06-21 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Waschmittel
US20090130718A1 (en) * 1999-02-04 2009-05-21 Diversa Corporation Gene site saturation mutagenesis
KR20020021398A (ko) 1999-07-22 2002-03-20 데이비드 엠 모이어 정의된 에피토프 구역에 아미노산 치환을 가진 서브틸리신프로테아제 변이체
CZ2002171A3 (cs) 1999-07-22 2002-06-12 The Procter & Gamble Company Proteinázový konjugát, čistící prostředek a prostředek osobní péče
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
CA2379712A1 (fr) 1999-07-22 2001-02-01 The Procter & Gamble Company Variants de protease subtilisine possedant des suppressions et des substitutions d'acides amines dans certaines regions de sites antigeniques
CN1100789C (zh) * 2000-01-13 2003-02-05 中国人民解放军第二军医大学 基因重组降钙素或降钙素类似物的制备方法
US20030170846A1 (en) * 2000-04-24 2003-09-11 Weisgerber David John Enzyme variants having one or more D-amino acid substitutions
ES2261491T3 (es) * 2000-09-04 2006-11-16 Cellpep S.A. Derivados de la maurotoxina, pi1 y hstx1.
US20040009498A1 (en) * 2002-01-14 2004-01-15 Diversa Corporation Chimeric antigen binding molecules and methods for making and using them
US20030219854A1 (en) * 2002-03-21 2003-11-27 Micrologix Biotech Inc. Methods for producing modified anti-infective peptides
US20040171070A1 (en) * 2002-05-20 2004-09-02 Ramagauri Bhikhabhai Peptide analysis using a solid support
CA2569707C (fr) * 2004-08-13 2013-09-24 F. Hoffmann-La Roche Ag Modification du c-terminal de polypeptides
JP5114201B2 (ja) * 2004-09-27 2013-01-09 サノフイ−アベンテイス・ドイチユラント・ゲー・エム・ベー・ハー 組み換えカルボキシペプチダーゼｂ
WO2007082890A1 (fr) * 2006-01-17 2007-07-26 N.V. Organon HYDROLYSE ENZYMATIQUE SÉLECTIVE D'ESTERS tert-BUTYLIQUES C-TERMINAUX DE PEPTIDES
US9133500B2 (en) * 2009-08-10 2015-09-15 MorphoSys A6 Screening strategies for the identification of binders
BR112014027861A2 (pt) 2012-05-11 2017-12-12 Novozymes As método de preparação de um mosto

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US5049656A (en) 1988-12-21 1991-09-17 Board Of Regents Of The University Of Nebraska Sequential peptide and oligonucleotide syntheses using immunoaffinity techniques
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