[go: up one dir, main page]

WO1995006132A3 - Identifying biologically active agents through culture color change - Google Patents

Identifying biologically active agents through culture color change Download PDF

Info

Publication number
WO1995006132A3
WO1995006132A3 PCT/US1994/009304 US9409304W WO9506132A3 WO 1995006132 A3 WO1995006132 A3 WO 1995006132A3 US 9409304 W US9409304 W US 9409304W WO 9506132 A3 WO9506132 A3 WO 9506132A3
Authority
WO
WIPO (PCT)
Prior art keywords
culture
cell
color
strains
agent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1994/009304
Other languages
French (fr)
Other versions
WO1995006132A2 (en
Inventor
Alex Andrianopoulos
Christine E Bulawa
Martin Goldway
Yigal Koltin
N Ronald Morris
Sean Christopher O'connor
Amos Oppenheim
David Michael Rothstein
William E Timberlake
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Georgia
University of Georgia Research Foundation Inc UGARF
Myco Pharmaceuticals Inc
Original Assignee
University of Georgia
University of Georgia Research Foundation Inc UGARF
Myco Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Georgia, University of Georgia Research Foundation Inc UGARF, Myco Pharmaceuticals Inc filed Critical University of Georgia
Publication of WO1995006132A2 publication Critical patent/WO1995006132A2/en
Publication of WO1995006132A3 publication Critical patent/WO1995006132A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/165—Yeast isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/72—Candida
    • C12R2001/725—Candida albicans

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a method of identifying a biologically active agent, which affects a targeted cellular component, whose existence or function is essential for cell viability, pathogenesis or drug resistance. This assay method includes the use of a mixed culture of first and second cell strains. The cell strains may be either strains of different cells or different strains of the same cell type. The two cell strains are distinguished by color differences. The first cell strain has a first color while the second cell strain displays a second color. The second cell strain is also more sensitive towards a class of biologically active agents than is the first cell strain. The first and second cell strains, and a candidate agent are mixed with a medium, appropriate for growth of the strains, forming a mixed assay culture. The culture is then exposed to conditions appropriate for growth. The initial proportions of the first and second cell strains are such that the culture predominantly favors the development of the second color. The biological activity of the agent is then determined from the color of the culture. Where the mixed culture retains the second color, the agent was not active against the target. However, where the culture predominantly becomes the first color, then the agent showed specific activity towards the target.
PCT/US1994/009304 1993-08-27 1994-08-19 Identifying biologically active agents through culture color change Ceased WO1995006132A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11326493A 1993-08-27 1993-08-27
US08/113,264 1993-08-27

Publications (2)

Publication Number Publication Date
WO1995006132A2 WO1995006132A2 (en) 1995-03-02
WO1995006132A3 true WO1995006132A3 (en) 1995-04-27

Family

ID=22348479

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1994/009304 Ceased WO1995006132A2 (en) 1993-08-27 1994-08-19 Identifying biologically active agents through culture color change

Country Status (1)

Country Link
WO (1) WO1995006132A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11415513B2 (en) 2015-07-15 2022-08-16 Universite Grenoble Alpes Method for determining the degree of sensitivity of a strain of fungus to an antifungal agent

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10513048A (en) * 1995-01-23 1998-12-15 マイクロサイド・ファーマシューティカルズ・インコーポレーテッド Screening for modulators of biomolecules
DE19713572A1 (en) 1997-04-02 1998-10-22 Hoechst Ag Method for screening antifungal substances
NO974299D0 (en) * 1997-09-18 1997-09-18 Forskningsparken I Aas As Method for stable labeling of microorganisms
US6280963B1 (en) 1997-11-07 2001-08-28 Millennium Pharmaceuticals, Inc. Essential fungal genes and their use
CA2232536A1 (en) * 1998-03-19 1999-09-19 Howard Bussey Antifungal drug screening using saccharomyces cerevisiae mannosyltransferase encoding genes
US6518035B1 (en) 1998-06-02 2003-02-11 Rosetta Inpharmatics, Inc. Targeted methods of drug screening using co-culture methods
US6720139B1 (en) 1999-01-27 2004-04-13 Elitra Pharmaceuticals, Inc. Genes identified as required for proliferation in Escherichia coli
US6589738B1 (en) 1999-11-09 2003-07-08 Elitra Pharmaceuticals, Inc. Genes essential for microbial proliferation and antisense thereto
US6783985B1 (en) 2000-02-18 2004-08-31 Elitra Pharmaceuticals Inc. Gene disruption methodologies for drug target discovery
US7266458B2 (en) 2000-03-06 2007-09-04 Bioseek, Inc. BioMAP analysis
US6763307B2 (en) 2000-03-06 2004-07-13 Bioseek, Inc. Patient classification
WO2001067103A1 (en) 2000-03-06 2001-09-13 Bioseek, Inc. Function homology screening
DE10261834A1 (en) * 2002-12-20 2004-07-08 Phenion Gmbh & Co. Kg High-throughput suitable screening process for the identification of active substances
CN115508316B (en) * 2021-06-23 2025-08-26 山东大学 A method for identifying drug-resistant Candida albicans and a kit for identifying the same

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0626453A1 (en) * 1993-05-25 1994-11-30 American Cyanamid Company Cytochrome P-450 reductase screen for ergosterol biosynthesis inhibitors

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0626453A1 (en) * 1993-05-25 1994-11-30 American Cyanamid Company Cytochrome P-450 reductase screen for ergosterol biosynthesis inhibitors

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
AM. J. ENOL. VITIC., vol. 42, no. 4, pages 295 - 300 *
BIOLOGICAL ABSTRACTS, vol. 90, no. 2, 15 July 1990, Philadelphia, PA, US; abstract no. 20976, C. GIL ET AL.: "Isolation and characterization of Candida albicans morphological mutants derepressed for the formation of filamentous hypha-type structures." page AB1119; *
CHEMICAL ABSTRACTS, vol. 107, no. 17, 26 October 1987, Columbus, Ohio, US; abstract no. 151030, H. F. JENKINSON ET AL.: "A mutant of Candida albicans deficient in beta-N-acetylglucosaminidase (chitobiase)." page 405; column 1; *
CHEMICAL ABSTRACTS, vol. 116, no. 17, 27 April 1992, Columbus, Ohio, US; abstract no. 172667, C. J. JACOBS ET AL.: "Effects of different killer yeasts on wine fermentations." page 730; column 1; *
CHEMICAL ABSTRACTS, vol. 78, no. 25, 25 June 1973, Columbus, Ohio, US; abstract no. 156516, S. NAGAI ET AL.: "Discrimination of the nonfoaming mutant from the parent yeast, Kyokai No. 7, by dyeing method." page 175; column 1; *
J. BACTERIOL., vol. 172, no. 5, pages 2384 - 2391 *
J. GEN. MICROBIOL., vol. 133, no. 8, pages 2097 - 2106 *
NIPPON JOZO KYOKAI ZASSHI, vol. 67, no. 11, pages 961 - 963 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11415513B2 (en) 2015-07-15 2022-08-16 Universite Grenoble Alpes Method for determining the degree of sensitivity of a strain of fungus to an antifungal agent
CN108026564B (en) * 2015-07-15 2022-10-25 格勒诺布尔-阿尔卑斯大学 Method for determining the degree of sensitivity of a fungal strain to an antifungal agent

Also Published As

Publication number Publication date
WO1995006132A2 (en) 1995-03-02

Similar Documents

Publication Publication Date Title
WO1995006132A3 (en) Identifying biologically active agents through culture color change
Burns Enzyme activity in soil: location and a possible role in microbial ecology
DE69424663T2 (en) REPORTER ENZYME RELEASE TECHNOLOGY: METHOD FOR DETECTING THE PRESENCE OF ASPARTATE PROTEASES AND OTHER ACTIVITIES OF HYDROLYTIC ENZYMES
Kerridge The Effect of Actidione and other Antifunǵal Aǵents on Nucleic Acid and Protein Synthesis in Saccharomyces carlsbergensis
AU672707B2 (en) Reagents for use in bioluminescence
Akpan et al. A rapid plate culture method for screening of amylase producing micro-organisms
US5523214A (en) Method of visually demonstrating the presence of microorganisms, identifying them, and testing them for sensitivity to antibiotics with redox indicators
Sims et al. Proteolytic activity under nitrogen or sulfur limitation
EP0347771A3 (en) Device for enhancing fluorescence and kinetics and methods of using the device
McCracken et al. The linear-plasmid-encoded toxin produced the yeast Pichia acaciae: characterization an comparison with the toxin of Kluyveromyce: lactis
ES8301503A1 (en) Method for making genetically modified microorganisms.
Sharma et al. The product of the regulatory gene of the proline catabolism gene cluster of Aspergillus nidulans is a positive-acting protein
Mendelsohn et al. Inhibition of ribonuclease efficacy of sodium dodecyl sulfate, diethyl pyrocarbonate, proteinase K and heparin using a sensitive ribonuclease assay
CA2542825A1 (en) Multimer sample pretreatment and immunoassay without boiling, for total adiponectin assessment
AU6997794A (en) Process for screening substances having a modulating effect on an interleukine-5-receptor-dependent cellular signal transmission path
Yawetz et al. Epoxide hydrase in Trypanosoma cruzi epimastigotes
Schreurs et al. Chemotropism and branching as alternative responses of Achlya bisexualis to amino acids
Ciferri et al. Ribosome specificity of protein synthesis in vitro
EP0231126A3 (en) Fluorescent dyes and biological and analytical uses thereof
WO1997011196A3 (en) Detection of nucleic acids in cells by thermophilic strand displacement amplification
Maw Ability of S-methyl-L-cysteine to annul the inhibition of yeast growth by L-ethionine and by S-ethyl-L-cysteine
Stallcup et al. Half-of-the-sites reactivity of yeast glyceraldehyde 3-phosphate dehydrogenase
DE60208787T2 (en) METHOD OF MEASURING POLYMERIZATION OF DNA AND APPLICATIONS OF THE METHOD
Schin et al. Studies of programmed salivary gland regression during larval-pupal transformation in Chironomus thummi: I. Acid hydrolase activity
Adewale et al. Organ distribution and kinetics of Glutathione transferase from African river prawn, Macrobrachium vollenhovenii (Herklots)

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): CA JP

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: CA