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WO1994028180A3 - Fruit with modified nadp-linked malic enzyme activity - Google Patents

Fruit with modified nadp-linked malic enzyme activity Download PDF

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Publication number
WO1994028180A3
WO1994028180A3 PCT/GB1994/001197 GB9401197W WO9428180A3 WO 1994028180 A3 WO1994028180 A3 WO 1994028180A3 GB 9401197 W GB9401197 W GB 9401197W WO 9428180 A3 WO9428180 A3 WO 9428180A3
Authority
WO
WIPO (PCT)
Prior art keywords
fruit
malic enzyme
nadp
enzyme activity
linked malic
Prior art date
Application number
PCT/GB1994/001197
Other languages
French (fr)
Other versions
WO1994028180A2 (en
Inventor
Colin Roger Bird
Graham Barron Seymour
Original Assignee
Zeneca Ltd
Colin Roger Bird
Graham Barron Seymour
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zeneca Ltd, Colin Roger Bird, Graham Barron Seymour filed Critical Zeneca Ltd
Priority to AU68042/94A priority Critical patent/AU6804294A/en
Publication of WO1994028180A2 publication Critical patent/WO1994028180A2/en
Publication of WO1994028180A3 publication Critical patent/WO1994028180A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8249—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Fruit characteristics are modified by transforming fruit-bearing plants with a DNA construct adapted to modify the activity of NADP-linked malic enzyme. Such DNA constructs may be sense or antisense constructs encoding at least part of the enzyme. DNA constructs comprising a DNA sequence corresponding to at least part of an NADP-linked malic enzyme gene obtainable from fruit are also provided.
PCT/GB1994/001197 1993-06-02 1994-06-02 Fruit with modified nadp-linked malic enzyme activity WO1994028180A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU68042/94A AU6804294A (en) 1993-06-02 1994-06-02 Fruit with modified nadp-linked malic enzyme activity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9311346.2 1993-06-02
GB939311346A GB9311346D0 (en) 1993-06-02 1993-06-02 Modified fruit

Publications (2)

Publication Number Publication Date
WO1994028180A2 WO1994028180A2 (en) 1994-12-08
WO1994028180A3 true WO1994028180A3 (en) 1995-02-02

Family

ID=10736484

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1994/001197 WO1994028180A2 (en) 1993-06-02 1994-06-02 Fruit with modified nadp-linked malic enzyme activity

Country Status (3)

Country Link
AU (1) AU6804294A (en)
GB (1) GB9311346D0 (en)
WO (1) WO1994028180A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6653535B1 (en) * 1999-05-28 2003-11-25 Pioneer Hi-Bred International, Inc. Methods for modulating water-use efficiency or productivity in a plant by transforming with a DNA encoding a NAPD-malic enzyme operably linked to a guard cell or an epidermal cell promoter
CN119639776A (en) * 2025-01-16 2025-03-18 沈阳农业大学 Nanguo pear PuNADP-ME gene, over-expression and silencing vector and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992017596A1 (en) * 1991-03-28 1992-10-15 Imperial Chemical Industries Plc Dna, dna constructs, cells and plants derived therefrom

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992017596A1 (en) * 1991-03-28 1992-10-15 Imperial Chemical Industries Plc Dna, dna constructs, cells and plants derived therefrom

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
annual meeting of the American Society of Plant Physiologists, Pittsburgh, Penn- sylvania, USA, August 1-5, 1992. *
F.L. FINGER ET AL.;: "Probes for isolation of tomato fruit NADP-malic enzyme cDNA clone", PLANT PHYSIOL., VOL. 99 (1 SUPPL.), 1992, pages 73 *
joint annual meeting of the American and Canadian Societies of Plant Physiologists, Minneapolis, Minnesota, USA, July 31- August 4, 1993. *
K.E. FRANKE AND D.O. ADAMS;: "N-terminal amino acid and partial cDNA sequences for tomato malic enzyme", PLANT PHYSIOL., VOL. 102 (1 SUPPL.), 1993, pages 72 *
M.S. RAJEEVAN ET AL.;: "Isolation and characterization of cDNA clones for NADP-malic enzyme from leaves of Flaveria: transcript abundance distinguishes C3, C3-C4 and C4 photosynthetic types", PLANT MOL. BIOL., vol. 17, 1991, pages 371 - 383 *
P.W. GOODENOUGH ET AL.;: "NADP-linked malic enzyme and malate metabolism in ageing tomato fruit", PHYTOCHEM., vol. 24, 1985, pages 1157 - 1162 *

Also Published As

Publication number Publication date
GB9311346D0 (en) 1993-07-21
AU6804294A (en) 1994-12-20
WO1994028180A2 (en) 1994-12-08

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