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WO1994026934A3 - Methode de detection du papillomavirus chez l'homme - Google Patents

Methode de detection du papillomavirus chez l'homme Download PDF

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Publication number
WO1994026934A3
WO1994026934A3 PCT/US1994/005085 US9405085W WO9426934A3 WO 1994026934 A3 WO1994026934 A3 WO 1994026934A3 US 9405085 W US9405085 W US 9405085W WO 9426934 A3 WO9426934 A3 WO 9426934A3
Authority
WO
WIPO (PCT)
Prior art keywords
assay
complexed
amplified
specific
solid phase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1994/005085
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English (en)
Other versions
WO1994026934A2 (fr
Inventor
Janice T Brown
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baxter Healthcare Corp
Original Assignee
Baxter Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Diagnostics Inc filed Critical Baxter Diagnostics Inc
Priority to CA002139623A priority Critical patent/CA2139623A1/fr
Priority to AU69456/94A priority patent/AU6945694A/en
Priority to EP94917931A priority patent/EP0655091A1/fr
Priority to JP6525595A priority patent/JPH07508891A/ja
Publication of WO1994026934A2 publication Critical patent/WO1994026934A2/fr
Publication of WO1994026934A3 publication Critical patent/WO1994026934A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/708—Specific hybridization probes for papilloma

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Méthode de détection en deux étapes se basant sur une sonde d'hybridation d'acide nucléique de certains types de papillomavirus chez l'homme (HPV) associés à une dysplasie et à une malignité des cellules cervicales. Ladite méthode comprend une étape d'hybridation à capture en phase liquide, dans laquelle on hybride à un réactif de capture biotinylé un ARN messager amplifié de gènes spécifiques E6/E7 obtenu à partir d'un spécimen biologique, de façon à préparer un complexe; la fixation du complexe de réactif de capture à une phase solide par réaction avec de la streptavidine immobilisée; une deuxième étape d'hybridation dans laquelle une sonde de détection conjugée à un enzyme spécifique au virus s'hybride avec le complexe d'ARN messager amplifié; enfin, la détection du complexe sonde de détection par production couleur ou fluorogène suivant le lavage de la phase solide et l'apport d'un substrat approprié chromogène ou fluorogène. La méthode de détection présente une sensibilité amplifiée par rapport aux procédés classiques, ainsi qu'une spécificité d'expression réelle d'oncogènes de HPV dans des spécimens cervicaux, n'indiquant pas uniquement la présence d'un virus.
PCT/US1994/005085 1993-05-06 1994-05-06 Methode de detection du papillomavirus chez l'homme Ceased WO1994026934A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002139623A CA2139623A1 (fr) 1993-05-06 1994-05-06 Test de detection des papillomavirus chez l'humain
AU69456/94A AU6945694A (en) 1993-05-06 1994-05-06 Human papillomavirus detection assay
EP94917931A EP0655091A1 (fr) 1993-05-06 1994-05-06 Methode de detection du papillomavirus chez l'homme
JP6525595A JPH07508891A (ja) 1993-05-06 1994-05-06 ヒト乳頭種ウイルス検出アッセイ

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5892093A 1993-05-06 1993-05-06
US08/058,920 1993-05-06

Publications (2)

Publication Number Publication Date
WO1994026934A2 WO1994026934A2 (fr) 1994-11-24
WO1994026934A3 true WO1994026934A3 (fr) 1995-01-26

Family

ID=22019729

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1994/005085 Ceased WO1994026934A2 (fr) 1993-05-06 1994-05-06 Methode de detection du papillomavirus chez l'homme

Country Status (5)

Country Link
EP (1) EP0655091A1 (fr)
JP (1) JPH07508891A (fr)
AU (1) AU6945694A (fr)
CA (1) CA2139623A1 (fr)
WO (1) WO1994026934A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7575918B2 (en) 1999-04-14 2009-08-18 The Penn State Research Foundation Tissue-specific and pathogen-specific ribozymes

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19506561C1 (de) * 1995-02-24 1996-10-10 Deutsches Krebsforsch Verfahren zur Früherkennung von HPV-assoziierten Karzinomen bzw. von hochgradigen, durch HPV-verursachten Dysplasien
DE19526717A1 (de) * 1995-07-21 1997-01-23 Florian Dr Med Heirler Verfahren zur Diagnose des Zervixkarzinoms
FR2737502B1 (fr) * 1995-07-31 1997-10-24 Genset Sa Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection
AU766744B2 (en) * 1995-11-15 2003-10-23 Gen-Probe Incorporated Nucleic acid probes complementary to human papillomavirus nucleic acid and related methods and kits
JP2000500342A (ja) 1995-11-15 2000-01-18 ジェン−プローブ・インコーポレーテッド ヒトパピローマウイルス核酸に相補的な核酸プローブおよび関連する方法およびキット
US6013488A (en) * 1996-07-25 2000-01-11 The Institute Of Physical And Chemical Research Method for reverse transcription
DE69737107T2 (de) 1996-11-20 2007-07-12 Introgen Therapeutics Inc., Austin Ein verbessertes verfahren zur produktion und reinigung von adenoviralen vektoren
US6623920B1 (en) 1999-07-09 2003-09-23 Gen-Probe Incorporated Detection of HIV-1 by nucleic acid amplification
AU7955800A (en) * 1999-10-25 2001-05-08 Wakunaga Pharmaceutical Co., Ltd Method of detecting target nucleic acid
AU5347101A (en) * 2000-04-13 2001-10-30 Univ South Carolina Tissue-specific and pathogen-specific toxic agents, ribozymes, dnazymes and antisense oligonucleotides, and methods of use thereof
US6447995B1 (en) 2000-10-04 2002-09-10 Genvec, Inc. Utilizing intrinsic fluorescence to detect adenovirus
GB0120938D0 (en) * 2001-08-29 2001-10-17 Norchip As Detection of human papillomavirus E7 mRNA
BR0306723A (pt) * 2002-01-07 2004-12-21 Norchip As Métodos in vitro para selecionar sujeitos humanos para avaliar seu risco de desenvolver carcinoma cervical, e para selecionar sujeitos humanos quanto à presença de hpv integrado ou um genoma de hpv epissÈmico modificado, método in vitro para identificar sujeitos humanos apresentado alterações anormais de células no cervix, e, kit para uso na detecção de transcrições de mrna dos genes l1 e e6 do hpv
US7354719B2 (en) 2004-12-08 2008-04-08 Gen-Probe Incorporated Detection of nucleic acids from multiple types of human papillomaviruses
CN101151379A (zh) 2005-01-14 2008-03-26 密执安州立大学董事会 检测生物样品中的人乳头瘤病毒的系统、方法和组合物
US7524631B2 (en) * 2005-02-02 2009-04-28 Patterson Bruce K HPV E6, E7 mRNA assay and methods of use thereof
US20070111960A1 (en) * 2005-03-04 2007-05-17 Advandx, Inc. High affinity probes for analysis of human papillomavirus expression
GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
US9714446B2 (en) 2010-02-11 2017-07-25 Nanostring Technologies, Inc. Compositions and methods for the detection of small RNAs
US9458484B2 (en) 2010-10-22 2016-10-04 Bio-Rad Laboratories, Inc. Reverse transcriptase mixtures with improved storage stability
AU2013205122B2 (en) 2012-10-11 2016-11-10 Gen-Probe Incorporated Compositions and Methods for Detecting Human Papillomavirus Nucleic Acid
WO2018059581A1 (fr) * 2016-09-30 2018-04-05 广州易活生物科技有限公司 Sonde permettant la détection de génotypage du papillomavirus humain au moyen d'un kit, utilisation et technique efirm
EP3849993A4 (fr) * 2018-09-10 2023-02-08 Memorial Sloan Kettering Cancer Center Procédés et compositions pour la détection d'adn de vph et le diagnostic et la surveillance de cancers associés au vph
WO2022067023A1 (fr) * 2020-09-24 2022-03-31 Mayo Foundation For Medical Education And Research Plates-formes de criblage

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0373352A2 (fr) * 1988-11-11 1990-06-20 BEHRINGWERKE Aktiengesellschaft Méthode pour détecter de l'ARNm épissée
EP0420260A2 (fr) * 1989-09-29 1991-04-03 F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft ADN marqué de biotine par la réaction en chaîne de polymérase et de son dépistage
EP0425217A2 (fr) * 1989-10-23 1991-05-02 Ciba Corning Diagnostics Corp. Essai d'hybridation pour l'ARN ribosomale de campylobacter
WO1991008312A1 (fr) * 1989-12-01 1991-06-13 Gene-Trak Systems Detection des transcriptions du vph
WO1991019812A1 (fr) * 1990-06-11 1991-12-26 Bio Merieux Procede de detection d'une sequence nucleotidique selon la technique d'hybridation sandwich
WO1992001815A1 (fr) * 1990-07-19 1992-02-06 Royal Free Hospital School Of Medicine Procede de diagnostic du virus du papillone humain de type 16 par la reaction de chaîne de polymerase
WO1992014847A2 (fr) * 1991-02-13 1992-09-03 Orgenics International Holdings B.V. Detection du palillomavirus humain a haut risque et a risque faible par amplification enzymatique d'adn
WO1993024658A1 (fr) * 1992-05-29 1993-12-09 Gen Trak, Inc. Sonde d'amplification de signal et procedes d'utilisation

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0373352A2 (fr) * 1988-11-11 1990-06-20 BEHRINGWERKE Aktiengesellschaft Méthode pour détecter de l'ARNm épissée
EP0420260A2 (fr) * 1989-09-29 1991-04-03 F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft ADN marqué de biotine par la réaction en chaîne de polymérase et de son dépistage
EP0425217A2 (fr) * 1989-10-23 1991-05-02 Ciba Corning Diagnostics Corp. Essai d'hybridation pour l'ARN ribosomale de campylobacter
WO1991008312A1 (fr) * 1989-12-01 1991-06-13 Gene-Trak Systems Detection des transcriptions du vph
WO1991019812A1 (fr) * 1990-06-11 1991-12-26 Bio Merieux Procede de detection d'une sequence nucleotidique selon la technique d'hybridation sandwich
WO1992001815A1 (fr) * 1990-07-19 1992-02-06 Royal Free Hospital School Of Medicine Procede de diagnostic du virus du papillone humain de type 16 par la reaction de chaîne de polymerase
WO1992014847A2 (fr) * 1991-02-13 1992-09-03 Orgenics International Holdings B.V. Detection du palillomavirus humain a haut risque et a risque faible par amplification enzymatique d'adn
WO1993024658A1 (fr) * 1992-05-29 1993-12-09 Gen Trak, Inc. Sonde d'amplification de signal et procedes d'utilisation

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
J.T. BROWN AND A.T. WORTMAN: "Rapid amplification of human Papillomavirus type 16 and 18 E6 and E7 mRNA by 3SR", DIAGNOSTIC MOLECULAR BIOLOGY: PRINCIPLES AND APPLICATIONS, PERSING ET AL. (ED.), 0(0), 13 May 1993 (1993-05-13), XXII+641P. AMERICAN SOCIETY FOR MICROBIOLOGY (ASM): WASHINGTON, DC,US;, pages 414 - 419 *
J.T. BROWN AND E. W. RADANY: "Development of a rapid assay for the detection of HPV transforming gene expression", ICAA, PROGRAM ABSTRACT, vol. 31, no. 0, 1991, pages 334 *
J.T. BROWN ET AL.: "Isothermal enzymatic amplification of HPV RNA using the 3SR reaction", J. CELL. BIOCHEM. SUPPL. VOL.111, NO. 5 (PART 2),, November 1990 (1990-11-01), ROCKEFELLER UNIV. PRESS, N.Y., US;, pages 56A *
T.R. GINGERAS ET AL.: "Unique features of the self-sustained sequence replication (3SR) reaction in the in vitro amplification of nucleic acids", ANNALES DE BIOLOGIE CLINIQUE, vol. 48, 1990, PARIS, FR;, pages 498 - 501 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7575918B2 (en) 1999-04-14 2009-08-18 The Penn State Research Foundation Tissue-specific and pathogen-specific ribozymes

Also Published As

Publication number Publication date
EP0655091A1 (fr) 1995-05-31
CA2139623A1 (fr) 1994-11-24
WO1994026934A2 (fr) 1994-11-24
JPH07508891A (ja) 1995-10-05
AU6945694A (en) 1994-12-12

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