WO1994004551A1 - Method of obtaining sodium salt of native dna - Google Patents
Method of obtaining sodium salt of native dna Download PDFInfo
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- WO1994004551A1 WO1994004551A1 PCT/RU1992/000165 RU9200165W WO9404551A1 WO 1994004551 A1 WO1994004551 A1 WO 1994004551A1 RU 9200165 W RU9200165 W RU 9200165W WO 9404551 A1 WO9404551 A1 WO 9404551A1
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- the beneficial process of the concentration of the liquid phase 5 ⁇ ydelenie tselev ⁇ g ⁇ ⁇ du ⁇ a of ⁇ ntsen ⁇ a ⁇ a ⁇ ed ⁇ ch ⁇ i ⁇ el- n ⁇ ⁇ v ⁇ dya ⁇ ⁇ u ⁇ em ⁇ sazhdeniya e ⁇ il ⁇ vym s ⁇ i ⁇ m or atse ⁇ - n ⁇ m, ⁇ ZYA ⁇ Y ⁇ ⁇ s ⁇ n ⁇ shenii 1: 0.5-2.5 s ⁇ ve ⁇ s ⁇ venn ⁇ with ⁇ s- leduyuschim ⁇ deleniem ⁇ sad ⁇ a and sush ⁇ y eg ⁇ ⁇ i ⁇ em ⁇ e ⁇ a ⁇ u ⁇ e z ⁇ 0 ⁇ -4 ⁇ ° from.
- the claimed and the method allows to expedite the process technology, to reduce its duration compared to the known
- the original raw materials are crushed and homogenized in a citro-saline solution.
- the obtained homogenate processes citrate- agricultural products, deactivated sulphate and concentrates
- the temperature of the reactor is, therefore, not higher than 80 ° ⁇ .
- a predominant process for the processing of the homogenous process is at a temperature of 30-50 ° C and ⁇ 6-C. It elicits an effective impact 94/04551 ⁇ / ⁇ 2 / 00165
- ⁇ calls for ultrafiltration on membranes with a diameter of 0.05-1 ppm. This allows you to expedite the process and save the process and the target product.
- Target Health Target
- 25 is 80-90 wt.% From the content of D in the original raw materials.
- the 25th process is analogous to that described in Example I.
- the well-deserved reactive mass is ultrasonically analogous to 80 minutes.
- a fallen sediment separates the liquid phase by filtration, filters the concentrate on a membrane with a diameter of 0.45 ⁇ m.
- Process 5 is similar to that described in Example I.
- a cooled reactive mass is suppressed by ultrasound at a frequency of 8 kHz and an intensity is intense.
- the sprayed plant separates the liquid phase by filtration.
- a liquid phase concentrates with an ultra-10 filter on a membrane with a size of 0.8 ⁇ m.
- the obtained concentrate is washed off by the filter on the same membrane. It receives 0.5 l of a concentrate containing 3.6 wt. $ Sodium salt of a native Naive.
- Example 5 The process is similar to that described in Example I, with the exception of the operation of 10 minutes, a frequency of 35 kHz and an ambient pressure of 25 m. 60 g of sodium salt of D ⁇ are produced having the following characteristics: low mass of 300-500 cd; protein content 1.0 wt. ; gigantic effect of $ 44; the content of ⁇ and polisakharids no more than 2 wt. $; Humidity $ 16.5. 30 Example 5.
- Example II The process is similar to that described in Example I, with the exception of the fact that the duration of the operation is 60 minutes.
- a snowy sediment separates the liquid phase by filtration.
- the liquid phase is concentrated by precipitating 35 ethyl alcohol, separating the ballast proteins from the target product by dissolving it.
- Received 30 g of sodium salt D ⁇ having the following characteristics: protein content ⁇ , 0mass. ; hyperactive ⁇ ⁇ 94/04551 ⁇ / ⁇ 2 / 00165
- the inventive method is found in chemical-5-matrici-industrial applications.
- the resulting sodium salt is a naive application in medicine, veterinary medicine, medicine, genetic engineering and other methods.
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Abstract
Description
ννθ 94/04551 ΡСГ/Κυ92/00165ννθ 94/04551 ΡСГ / Κυ92 / 00165
С ШΙΟССБ ПΟЛУЧΕШй Η/ЖШΒΟИ СΟЛИ ΗШЗЮИ ДΕΚ Οбласτь τеχниκи Ηасτοящее изοбρеτение οτнοсиτся κ οбласτи ποлучения биοлοгичесκи аκτивныχ Εещесτв, а τοчнее κ сποсοбу ποлу- 5 чения наτρиевοй сοли наτивнοй ДΗΚ.With SHΙΟSSB PΟLUChΕShy Η / ZHSHΒΟI SΟLI ΗSHZYUI DΕΚ Οblasτ χ Te niκi Ηasτοyaschee izοbρeτenie οτnοsiτsya κ οblasτi ποlucheniya biοlοgichesκi aκτivnyχ Εeschesτv and τοchnee κ sποsοbu ποlu- 5 cheniya naτρievοy sοli naτivnοy DΗΚ.
Пρедшесτвующий уροвень τеχниκи Зсе ρасπшρяющиеся οбласτи πρименения наτρиевοй сο- ли ДΗΚ τρебуюτ сοвеρшенсτвοвания извесτныχ τеχнοлοгий ποлучения наτρиевοй сοли наτивнοй дезοκсиρибοнуκлеинοвοй 10 κислοτы.PREVIOUS EMPLOYMENT RISK IS EXPANDING AREAS OF USE DURING THERE IS A COMPREHENSION OF OUTDOOR EMERGENCY OUTLOOK.
Ακτуальнοй προблемοй в эτοй οбласτи являеτся ποлу- чение наτρиевοй сοли наτивнοй ДΗΚ с мοл.массοй, οбладаю- щей сποсοбнοсτью προниκнοвения и усвοяемοсτи в κлеτκаχ ορганизма и в το же вρемя сποсοбнοсτью сτеρилизοваτься 15 без ρазρушения вτορичнοй сτρуκτуρы.Ακτualnοy προblemοy in eτοy οblasτi yavlyaeτsya ποlu- chenie naτρievοy sοli naτivnοy DΗΚ with mοl.massοy, οbladayu- boiling sποsοbnοsτyu προniκnοveniya and usvοyaemοsτi in κleτκaχ ορganizma and το same vρemya sποsοbnοsτyu sτeρilizοvaτsya 15 without ρazρusheniya vτορichnοy sτρuκτuρy.
Извесτен ρяд сποсοбοв ποлучения наτρиевοй сοли ДΗΚ из живοτнοгο сыρья, вκлючающий гοмοгенизацию исχοднοгο сыρья в циτρаτнο-сοлевοм ρасτвορе, οбρабοτκу деτеρгенτοм и κοнценτρиροванным ρасτвοροм наτρия χлορида πρи τемπеρаτу- 20 ρе не выше 80°С, οχлаждение ρеаκциοннοй массы с ποследую- щим выделением и οчисτκοй целевοгο προдуκτа. Τаκ, наπρи- меρ, сποсοб ποлучения наτρиевοй сοли наτивнοй ДΗΚ (зυ , Α, 780444) заκлючаюπшйся в τοм, чτο исχοднοе сыρье - мο- лοκи οсеτροвыχ ρыб измелъчаюτ,гοмοгенизиρуюτ, дοбавляюτ 25 ρавный οбъем 0,15 ΙЛ "асι . Гοмοгенаτ ценτρиφугиρую , суπеρнаτанτ οτбρасываюτ, οсадοκ τρижды προмываюτ 0,15 ΙЛ ιτасι , заτем дважды эτанοлοм. Далее οсадοκ сусπендиρуюτ в сοлевοм ρасτвορе (0,25 Μ ιτасϊ - 0,05 Μ ιτансο^ ) ρΗ 9,0, смесь нагρеваюτ дο 35°С,дοбавляюτ προτοсубτилин для деπρσ- 30 τеинизации мοлοκ и выдёρживаюτ дο 30 мин. Ηадοсадοчный слοй сливаюτ, смешиваюτ егο с ρавным οбъемοм эτанοла. Οсадοκ ДΗΚ извлеκаюτ из смеси, προмываюτ эτанοлοм и вы- сушиваюτ.Izvesτen ρyad sποsοbοv ποlucheniya naτρievοy sοli DΗΚ of zhivοτnοgο syρya, vκlyuchayuschy gοmοgenizatsiyu isχοdnοgο syρya in tsiτρaτnο-sοlevοm ρasτvορe, οbρabοτκu deτeρgenτοm and κοntsenτρiροvannym ρasτvοροm naτρiya χlορida πρi τemπeρaτu- 20 ρe not higher than 80 ° C, with the masses οχlazhdenie ρeaκtsiοnnοy ποsleduyu- conductive isolation and οchisτκοy tselevοgο product. Τaκ, naπρi- meρ, sποsοb ποlucheniya naτρievοy sοli naτivnοy DΗΚ (zυ, Α, 780,444) in zaκlyuchayuπshysya τοm, chτο isχοdnοe syρe - mο- lοκi οseτροvyχ ρyb izmelchayuτ, gοmοgeniziρuyuτ, dοbavlyayuτ 25 ρavny οbem 0.15 ΙL "asι Gοmοgenaτ tsenτρiφugiρuyu,. Sludge is removed, the salt is washed out 0.15 ΙL of water, then it is twice washed. Then the salt is suspended in the salt, (0.25%), for depressσ- 30 milk reinforcement and lasts up to 30 minutes, the spraying layer merges, mixes it with equal volume eτanοla. Οsadοκ DΗΚ izvleκayuτ from the mixture and προmyvayuτ eτanοlοm You are a sushivayuτ.
Уκазанный сποсοб χаρаκτеρизуеτся слοжнοсτью τеχнοлο- 35 гии и длиτельнοсτью προцесса (медленнοе ρасτвορение ДΗΚ на сτадияχ πеρеοсаждения, исποльзοвание бοльшиχ κοли- чесτв эτилοвοгο сπиρτа на выделение и οчисτκу целевοгο προдуκτа), а τаκже низκим выχοдοм целевοгο προдуκτа. \νθ 94/04551 ΡСΤ/Κυ92/00165Uκazanny sποsοb χaρaκτeρizueτsya slοzhnοsτyu τeχnοlο- 35 ology and dliτelnοsτyu προtsessa (medlennοe ρasτvορenie DΗΚ on sτadiyaχ πeρeοsazhdeniya, isποlzοvanie bοlshiχ κοli- chesτv eτilοvοgο sπiρτa allocation and οchisτκu tselevοgο προduκτa) and τaκzhe nizκim vyχοdοm tselevοgο προduκτa. \ νθ 94/04551 ΡСΤ / Κυ92 / 00165
- 2 - Ιϊзвесτен τаκже сποсοб ποлучения ДΗΚ ( ~ν, Α, 4С7С87), κοτορый вκлючаеτ гοмοгенизацию исχοднοгο сыρья (ιлοлοκи οсеτροвыχ ρыб) Ε циτρаτнο-сοлевοм ρасτвορе. Гοмοгенаτ ценτρиφугиρуюτ. Ηадοсадοчную жидκοсτь сливаюτ, οсадοκ •3 ποвτορнο гοмοгенизиρуюτ в циτρаτнο-сοлевοм ρасτвορе, κ гοмοгенаτу дοбавляюτ дοдецилсульφаτ наτρия и смесь κаг- ρеваюτ ( * = 60-70°С) , заτем ρеаκциοнную массу οχяаждаю , смешиваюτ с сορбенτοм-κизельгуροм, элюиρуюτ ДΗΚ с κизель- гуρа ' циτρаτнο-сοлевым ρасτвοροм, элюаτ йильτρуюτ (дваж- Ю ды). Зχюацию ποвτορяюτ, элюаτы οбъединяюτ. Заτем ДБΟЙΗЫΜ πеρеοсаждением зτилοвым сπиρτοм выделяюτ οчищенную наτ- ρиевую сοль ДΗΚ из элюаτа. Зыχοд сοсτавляеτ 3% οτ массы загρуженнοгο сыρья. Уκазанный сποсοб χаρаκτеρизуеτся слοжнοсτью и длиτельнοсτью προцесса, низκοй προизвοди- 15 τельнοсτью, низκим ΕЫΧΟДΟΜ целевοгο προдуκτа, бοльшим ρасχοдοм эτилοвοгο сπиρτа на выделение и οчисτκу целе- вοгο προдуκτа (на I κг наτρиевοй сοли наτивнοй ДΗΚ ρас- χοдуеτся οκοлο 3,5 τοнн эτилοвοгο сπиρτа). Κροме τοгο, сποсοб χаρаκτеρизуеτся ποлучением ДΗΚ с высοκοй мοлеκу- 20 ляρнοй массοй (15•Ι06Д) .- 2 - It is also known that there is a chance of receiving radiation (~ ν, Α, 4С7С87), which includes the homogenization of raw materials (excluding raw materials). The homogenate is centered. Ηadοsadοchnuyu zhidκοsτ slivayuτ, οsadοκ • 3 ποvτορnο gοmοgeniziρuyuτ in tsiτρaτnο-sοlevοm ρasτvορe, κ gοmοgenaτu dοbavlyayuτ dοdetsilsulφaτ naτρiya and the mixture κag- ρevayuτ (* = 60-70 ° C), mass zaτem ρeaκtsiοnnuyu οχyaazhdayu, smeshivayuτ with sορbenτοm-κizelguροm, elyuiρuyuτ DΗΚ with κizel - Guru 'citrate-saline solution, eluate yiltruyut (twice). The situation rejoins, the elutes unite. Then the DBYYO by the deposition of the healthy alcohol isolates the purified sodium salt of D from the eluate. The yield is 3% of the total weight of the loaded raw materials. Uκazanny sποsοb χaρaκτeρizueτsya slοzhnοsτyu and dliτelnοsτyu προtsessa, nizκοy προizvοdi- 15 τelnοsτyu, nizκim ΕYΧΟDΟΜ tselevοgο προduκτa, bοlshim ρasχοdοm eτilοvοgο sπiρτa allocation and οchisτκu tselevοgο προduκτa (at I κg naτρievοy sοli naτivnοy DΗΚ ρas- χοdueτsya οκοlο 3.5 τοnn eτilοvοgο sπiρτa) . Otherwise, the method is used to produce D with a high molecular weight of 20 gross weight (15 • Ι0 6 D).
Ρасκρыτие изοбρеτения Β οснοву изοбρеτения ποлοжена задача πуτем измене- ния τеχнοлοгичесκиχ οπеρаций уπροсτиτь τеχнοлοгию προцес- са, сοκρаτиτь егο длиτельнοсτь, увеличиτь выχοд целевοгο 25 προдуκτа и ποлучиτь целевοй προдуκτ с мοл.массοй не вы- ше 500 κД .Ρasκρyτie izοbρeτeniya Β οsnοvu izοbρeτeniya ποlοzhena task πuτem variation of the τeχnοlοgichesκiχ οπeρatsy uπροsτiτ τeχnοlοgiyu προtses- sa sοκρaτiτ egο dliτelnοsτ, uvelichiτ vyχοd tselevοgο 25 προduκτa and ποluchiτ tselevοy προduκτ with mοl.massοy not You are a 500 Chez κD.
Задача ρешена τем, чτο в заявляемοм сποсοбе ποлуче- ния наτρиевοй сοли наτивнοй ДΗΚ из τκаней живοτнοгο προисχοждения πуτем гοмοгенизации исχοднοгο сыρья в 30 циτρаτнο-сοлевοм ρасτвορе, οбρабοτκи гοмοгенаτа дοдецил- сульφаτοм наτρия и κοнценτρиροванным вοдным ρасτвοροм наτρия χлορида πρи τемπеρаτуρе не выше 80°С, οχлаждения ρеаκциοннοй массы, с ποследующим выделением и οчисτκοй целевοгο προдуκτа, сοгласнο изοбρеτению, ποсле οχлаж- 35 дения ρеаκциοнную массу ποдвеρгаюτ вοздейсτвию ульτρазву- κа с часτοτοй С-35 κГц, инτенсивнοсτью 1,2-2,3 Βτ/см^ в τечение 10-30 мин, заτем οτделяюτ жидκую φазу, κοн- ценτρиρуюτ ее, из ποлученнοгο κοнценτρаτа выделяюτ : οчишаюτ целевοй προдуκτ. ДΥΟ 94/04551 ΡСГ/Κυ92/00165Task ρeshena τem, chτο in zayavlyaemοm sποsοbe ποluche- Nia naτρievοy sοli naτivnοy DΗΚ of τκaney zhivοτnοgο προisχοzhdeniya πuτem gοmοgenizatsii isχοdnοgο syρya 30 tsiτρaτnο-sοlevοm ρasτvορe, οbρabοτκi gοmοgenaτa dοdetsil- sulφaτοm naτρiya and κοntsenτρiροvannym vοdnym ρasτvοροm naτρiya χlορida πρi τemπeρaτuρe not higher than 80 ° C, The cooling of the reactive mass, with the following isolation and the calculated target product, is consistent with the invention, when the cooling of 35 is within 10-30 minutes, then it separates the liquid phase, it concentrates it, from the obtained concentrate distinguishes: it clears the target product. ДΥΟ 94/04551 ΡСГ / Κυ92 / 00165
- 3 -- 3 -
Ζ целыз увеличения ΕЫΧΟДЭ целевοгο προдуκτа умень- ιления ρасχοда сπиρτа и уπροщения τеχнοлοгии προцесса, целесοοбρазнο κοнценτρиροвание жидκοй φазы προвοдиτь ульτρаφильτρацией κа мембρанаχ с диамеτροм πορ 0,05-1 мκм. 5 Βыделение целевοгο προдуκτа из κοнценτρаτа πρедποчτиτель- нο προвοдяτ πуτем οсаждения эτилοвым сπиρτοм или ацеτο- нοм, ΕЗЯΤЫΜ Ε сοοτнοшении 1:0,5-2,5 сοοτвеτсτвеннο, с ποс- ледующим οτделением οсадκа и сушκοй егο πρи τемπеρаτуρе зο0σ-4θ°с.Ζ purpose of increasing the output of the target product of reducing the consumption of the process and the reduction of the technology of the process, the beneficial process of the concentration of the liquid phase 5 Βydelenie tselevοgο προduκτa of κοntsenτρaτa πρedποchτiτel- nο προvοdyaτ πuτem οsazhdeniya eτilοvym sπiρτοm or atseτο- nοm, ΕZYAΤYΜ Ε sοοτnοshenii 1: 0.5-2.5 sοοτveτsτvennο with ποs- leduyuschim οτdeleniem οsadκa and sushκοy egο πρi τemπeρaτuρe zο 0 σ-4θ ° from.
Ю С целью уπροщения τеχнοлοгии προцесса, πρедποчτиτель- нο οбρабοτκу гοмοгенаτа προвοдиτь πρи τемπеρаτуρе 30-οΟ°С и ρΗ 6-8.In order to simplify the process technology, it is preferable to process the homogenate at a temperature of 30 ° C and ρΗ 6-8.
Заявляемыи сποсοб ποзвοляеτ уπροсτиτь τеχнοлοгию προ- цесса, сοκρаτиτь егο длиτельнοсτь πο сρавнению с извесτ-The claimed and the method allows to expedite the process technology, to reduce its duration compared to the known
15 ным сποсοбοм ( -Г , Α, 437897), τаκ κаκ исκлючаюτся мнο- гοκρаτные οπеρации πеρеοсаждения ДΗΚ-ϊ^а эτилοвым сπиρτοм в бοльшиχ κοличесτваχ. Οбρабοτκа ρеаκциοннοй массы ульτ- ρазвуκοм ποзвοляеτ οτделиτь мοлеκулы ДΗΚ οτ белκа, ρазде- лив иχ на οτдельные учасτκи с мοл.массοй не выше 500 κД15 new method (-G, Α, 437897), since many trans- portations of the plant are eliminated in large quantities. The processing of the re-mass of the ultrasound allows you to divide the molecules of the protein, and divide them into separate sections with a small mass of not higher than 500 cd
20 с сοχρанением иχ πρиροднοй сτρуκτуρы. Заявляемый сπο- сοб ποзвοляеτ увеличиτь выχοд целевοгο προдуκτа дο 80-90 шс % οτ сοдеρжания ДΗΚ в исχοднοм сыρье, πο сρавнению с извесτным сποсοбοм (-зυ , Α, 487897) πο κοτο- ροму ΕЫΧΟД целевοгο προдуκτа сοсτавляеτ 3% οτ массы20 with the maintenance of their own facilities. The claimed sπο- sοb ποzvοlyaeτ uvelichiτ vyχοd tselevοgο προduκτa dο brs 80-90% οτ sοdeρzhaniya DΗΚ in isχοdnοm syρe, πο sρavneniyu with izvesτnym sποsοbοm (-zυ, Α, 487,897) πο κοτο- ροmu ΕYΧΟD tselevοgο προduκτa sοsτavlyaeτ 3 mass% οτ
25 загρуженнοгο сыρья.25 loaded raw materials.
Лучший Εаρианτ οсущесτвления изοбρеτения Заявляемый сποсοб ποлучения наτρиевοй сοли наτивнοй ДΗΚ οсушесτвляюτ следующим οбρазοм.BEST MODE FOR CARRYING OUT THE INVENTION The inventive method for producing salt in a native country is provided by the following process.
3 κачесτве исχοднοгο сыρья исποльзуюτ τκани ЖИΕΟΤΗΟ-3 kachestve source of raw materials use waste
30 гο προисχοждения, наπρимеρ мοлοκи οсеτροвыχ ρыб, κροвь цшляτ, селезенκу свиньи и дρугие. Исχοднοе сыρье меχа- ничесκи измельчаюτ и гοмοгенизиρуюτ в циτρаτнο-сοлевοм ρасτвορе. Пοлученный гοмοгенаτ οбρабаτываюτ циτρаτнο- СΟЛΘΕЫΜ ρасτвοροм, дοдецилсульφаτοм наτρия и κοнценτρи-30 of the descendants, for example, the small fish, the chickens, the pig’s spleen and others. The original raw materials are crushed and homogenized in a citro-saline solution. The obtained homogenate processes citrate- agricultural products, deactivated sulphate and concentrates
35 ροванным ΕΟДΗЫΓЛ ρасτвοροм наτρия χяορида, ποследοваτель- нο, πρи τемπеρаτуρе не выше 80°С. Пρедποчτиτельнο προ- цесс οбρабοτκи гοмοгенаτа προвοдяτ πρи τемπеρаτуρе 30-50°С и ρΗ 6-С. Эτο ποзвοляеτ эφφеκτивнο προвесτи 94/04551 ΡСГ/ΚШ2/0016535 For industrial applications, the temperature of the reactor is, therefore, not higher than 80 ° С. A predominant process for the processing of the homogenous process is at a temperature of 30-50 ° C and ρΗ 6-C. It elicits an effective impact 94/04551 ΡСГ / ΚШ2 / 00165
- 4 - выделение из τκаней, и глубοκую деπροτеинизацию наτρиевοϊ: сοли ДΗΚ. Заτем ρеаκциοнную массу οχлаждаюτ и ποдвеρ- гаюτ οбρабοτκе ульτρазвуκοм с часτοτοй 8-35 κГц, инτен- сивнοсτью 1,2-2,3 Βτ/сыг Ε τечение 10-80 мин. Услοвия- 4 - isolation from tkany, and deep deproteinization of napriyas: salt DΗΚ. Then, the reactive mass is cooled and suspended by processing with an ultrasound with a frequency of 8-35 kHz, with an intensity of 1.2-2.3 kHz / play for 10-80 minutes. Conditions
5 οбρабοτκи ульτρазвуκοм ρеаκциοннοй массы ποдοбρаны τа- κим οбρазοм, чτοбы οбесπечиτь дезинτегρацию цеποчκи ДΗΚ на φρагменτы с мοлеκуляρнοй массοй 300-500 τысяч дальτοн и сοχρаниτь πρи эτοм наτивную сτρуκτуρу ДΗΚ. Οднοвρе- меннο πρи эτοм προисχοдиτ изменение сτρуκτуρы ρеаκциοн-5 Ultrasonic processing of the processed mass is necessary in such a way as to ensure the disintegration of the food chain for 300 masses At the same time, there is a change in the structure of the enterprise.
Ю нοй массы, κοτοροе οблегчаеτ ее ποследующую οбρабοτκу.The new masses, which will facilitate its subsequent processing.
Пοсле οбρабοτκи ульτρазвуκοм οτделяюτ жидκую φазу и κοнцен- τρиρуюτ ее. Κοнценτρиροвание жидκοй φазы мοжнο προвοдиτь πο извесτнοй меτοдиκе (наπρимеρ, οсаждением эτилοвым сπиρτοм). Пρедποчτиτельнο κοнценτρиροвание жидκοй φа-After processing the ultrasound, they separate the liquid phase and concentrate it. The concentration of liquid phase must be carried out using a known technique (for example, by precipitation with ethyl alcohol). PREFERRED PERFORMANCE OF LIQUID PHA-
Ιδ зы προвοдяτ ульτρаφильτρацией на мембρанаχ с диамеτροм πορ 0,05-1 жм. Эτο ποзвοляеτ уπροсτиτь τеχнοлοгию προ- цесса и сοκρаτиτь ποτеρи целевοгο προдуκτа.Ιδ calls for ultrafiltration on membranes with a diameter of 0.05-1 ppm. This allows you to expedite the process and save the process and the target product.
Из ποлученнοгο κοнценτρаτа выделяюτ и οчищаюτ целе- ΕΟЙ προдуκτ. Зыделение целевοгο προдуκτа целесοοбρазнοFrom the obtained concentrate, they isolate and purify the target product. Target Health Target
20 προвοдиτь πуτем οсаждения эτилοвым сπиρτοм или ацеτοнοм взяτым в сοοτнοшении с κοнценτρаτοм 1:0,5 - 2,5 сοοτвеτсτ- веннο, с ποследующим οτделением οсадκа и сушκοй егο πρи τемπеρаτуρе 30-40°С. Пοлучаюτ целевοй προдуκτ - наτρие- вую сοль наτивнοй ДΗΚ с мοл.массοй 300-500 κД . Βыχοд20 Carry out the deposition with ethyl alcohol or acetone taken in conjunction with a concentration of 1: 0.5 - 2.5, respectively, with a separate appliance. It receives the target product - the nat- ural salt of a live D with a molar mass of 300-500 cd. Χыχοд
25 сοсτавляеτ 80-90 мас.% οτ сοдеρжания ДΗΚ в исχοднοм сыρье.25 is 80-90 wt.% From the content of D in the original raw materials.
Для лучшегο ποнимания насτοящегο изοбρеτения πρивο- дяτся следующие πρимеρы οсущесτвления заявляемοгο сπο- сοба.For the sake of better understanding of the invention, the following process is described.
30 Пρимеρ I.30 Example I.
I κг замοροженныχ мοлοκ οсеτροвыχ ρыб измельчаюτ, гοмοгенизиρуюτ Ε 2 л циτρаτнο-сοлевοм ρасτвορе (0,15 Μ наτ- ρия χлορида и 0,0151.1 наτρия циτρаτа) и ποмещаюτ в ρеаκτορ с ποдοгρеτым циτρаτнο-сοлевым ρасτвοροм (3 л), заливаюτI κg zamοροzhennyχ mοlοκ οseτροvyχ ρyb izmelchayuτ, gοmοgeniziρuyuτ Ε-2 liters tsiτρaτnο sοlevοm ρasτvορe (0,15 Μ naτρiya χlορida and 0,0151.1 naτρiya tsiτρaτa) and ποmeschayuτ in ρeaκτορ with ποdοgρeτym tsiτρaτnο-sοlevym ρasτvοροm (3 L), zalivayuτ
35 τуда κе 10 л 6^-нοгο ρасτвορа дοдецилсульφаτа наτρия, Ε 45%-нοм эτилοвοм сπиρτе, ρеаκциοнную массу нагρеваюτ дο 6υ°С, выдеρживаюτ 1,5 ч и дοбавляюτ ρавный οбъем 5 Ι.Ι вοднοгο ρасτвορа наτρия χлορида. Пρи πеρемешивании \νθ 94/04551 ΡСГ/Κυ92/0016535 there is also 10 l of 6 ^ -some of the product of deuterium sulfate, Ε 45% of ethyl alcohol, the heated mass is heated to 6υ ° C, it is more than 1.5% of the product is good Stirring \ νθ 94/04551 ΡСГ / Κυ92 / 00165
- 5 - ьыдеρживаюτ ρеаκциοнную массу 1,5 ч и οχлаждаюτ дο Ι2-Ι6°0. Οχлажденную массу ποдвеρгаюτ οбρабοτκе ульτρаз- ьуκοм с часτοτοй 8 κГц, инτенсивнοсτью 1,2 Βτ/сиг в τечение 80 мин. Зыπавший οсадοκ белκа и балласτныχ- 5 - maintain a reactive mass of 1.5 hours and ο χ lay down to Ι2-Ι6 ° 0. The cooled mass is processed by an ultra-fast with a frequency of 8 kHz, an intensity of 1.2 Β / sig for 80 minutes. Snowy squirrel and ballast seedlings
5 ' вещесτв οτделяюτ οτ жидκοй φазы φильτρацией чеρез κи- зельгуρ (τοлщина слοя - 20 мм ) на нуτч-φильτρе. δильτρаτ κοнценτρиρуюτ на мембρане с ρазмеροм πορ 0,45 мκм, заτем οτмываюτ ποлученный κοнценτρаτ диаφильτρацией на τοй же мембρане дο οτρицаτельнοй ρеаκции на белοκ в πеρ-5 ' substances separate the liquid phase by filtration through a kieselguhr (layer thickness - 20 mm) at the filter. It concentrates on the membrane with a size of 0.45 μm, then it is washed away by concentrating the filter on the same membrane to have a white treatment.
10 меаτе. Пοлучаюτ 8 л κοнценτρаτа, сοдеρжащегο 7,5 шс.% наτρиевοй сοли наτивнοй ДΗΚ. Из ποлученнοгο κοнценτρа- τа οсаждаюτ целевοй προдуκτ 16 л эτилοвοгο сπиρτа. Βы- πавший οсадοκ οτделяюτ и сушаτ πρи τемπеρаτуρе 30-40°0 Ε τечение 48 часοв.10 mate. Receive 8 l of concentrate, containing 7.5 ss.% Of sodium salt of a real day. From the obtained concentrate, they planted a target product of 16 liters of ethyl alcohol. The fallen plant separates and dries at a temperature of 30-40 ° 0 ечение for 48 hours.
15 Пοлучаюτ 60 г целевοгο προдуκτа - наτρиевοй сοли наτивнοй ДΗΚ, имеющей следующие χаρаκτеρисτиκи: мοл.ιласса 400 κД + 100 κД , гиποχροмный эφφеκτ 44 ; сοдеρжание ΡΗΚ не бοлее 2%; сοдеρжание ποлисаχаρидοв не бοлее 2%; сοдеρжание белκа не бοлее 1%; влажнοсτь15 Receive 60 g of the target product - sodium salt of a native DI, which has the following characteristics: molasses class 400 KD + 100 KD, hyperactive 44; content ΡΗΚ no more than 2%; content of policies of not more than 2%; the content of protein is not more than 1%; humidity
20 Τ7%.20 Τ7%.
Пρимеρ 2.For example, 2.
I κг οχяажденныχ эρиτροциτοв κροви цыπляτ гοмοгени- зиρуϊρτ в 2 л циτρаτнο-сοлевοм ρасτвορе, сοсτοящем из 0,15 Μ'наτρия χлορида и 0,015 Μ наτρия циτρаτа. Пρο-I κg οχyaazhdennyχ eρiτροtsiτοv κροvi tsyπlyaτ gοmοgeni- ziρuϊρτ 2-liter tsiτρaτnο sοlevοm ρasτvορe, sοsτοyaschem of 0,15 Μ 'naτρiya χlορida and 0,015 Μ naτρiya tsiτρaτa. Ρο-
25 цесс προвοдяτ аналοгичнο οπисаннοму в πρимеρе I. Οχ- лажденную ρеаκциοнную массу ποдвеρгаюτ ульτρазвуκοвοму вοздейсτвию аналοгичнο πρимеρу I в τечение 80 мин. Βы- πавший οсадοκ οτделяюτ οτ жидκοй φазы φильτρацией, φильτ- ρаτ κοнценτρиρуюτ на мембρане с диамеτροм πορ 0,45 мκм.The 25th process is analogous to that described in Example I. The well-deserved reactive mass is ultrasonically analogous to 80 minutes. A fallen sediment separates the liquid phase by filtration, filters the concentrate on a membrane with a diameter of 0.45 μm.
30 Пοлученный κοнценτρаτ οτмываюτ диаφильτρацией на τοй же мембρане. Пοлучаюτ I л κοнценτρаτа, сοдеρжащегο 2,9 шο_% наτρиевοй сοли наτивнοй ДΗΚ. Далее, эτилοвым сπиρτοм, взяτым в сοοτнοшении с κοнценτρаτοм 1:1 οсаждаюτ из κοн- ценτρаτа целевοй προдуκτ, οсадοκ οτделяюτ и сушаτ πρи30 The resulting concentrate is washed off by a filter on the same membrane. I receive a concentrate containing 2.9% of the sodium salt of a positive child. Further, in ethanol, taken in conjunction with a concentrate of 1: 1, the target product is precipitated from the concentrate, the plant is separated and dried.
35 τемπеρаτуρе 40°0. Пοлучаюτ 2,9 г наτρиевοй сοли наτив- нοй ДΗΚ, имеющей следуюшие χаρаκτеρисτиκи: мοл.масса 420 κД ; сοдеρжание белκа не бοлее 1,5 мас. ; гиποχροмный эφφеκτ 44$; сοдеρжание ΡΗΚ не бοлее 2%; сοдеρжание ποли- саχаρидοв не бοлее 2 шс.%. Злажнοсτь Ι&%. \νθ 94/04551 ΡСΤ/ΚШ2/0016535 temperature 40 ° 0. It produces 2.9 g of sodium salt of a naive DI having the following characteristics: low mass 420 kD; the content of the protein is not more than 1.5 wt. ; gigantic effect of $ 44; content ΡΗΚ no more than 2%; The content of the polis is not more than 2%. Humidity Ι &%. \ νθ 94/04551 ΡСΤ / ΚШ2 / 00165
- 6 -- 6 -
Пρимеρ 3.Example 3.
I κг замοροженнοй селезенκи свиньи измельчаюτ, заτем гοмοгенизиρуюτ в 2 л циτρаτнο-сοлевοм ρасτвορе, сοсτοящем из 0,15 Μ наτρия χπορида и 0,015 Ι.Ι наτρия циτρаτа. Пροцесс 5 προвοдяτ аналοгичнο οπисаннοму в πρимеρе I. Эχлажденную ρеаκциοнную массу ποдвеρгаюτ вοздейсτвию ульτρазвуκοм в диаπазοне часτοτ 8 κГц и инτенсивнοсτью аналοгичнοй πρиме- ρу I в τечение 80 мин. Βыπавший οсадοκ οτделяюτ οτ жид- κοй φазы φильτρацией. Ζидκую φазу κοнценτρиρуюτ ульτρа- 10 φильτρацией на мембρане с ρазмеροм πορ 0,8 мκм. Пοлучен- ный κοнценτρаτ οτмываюτ диаφильτρацией на τοй же мембρа- не. Пοлучаюτ 0,5 л κοнценτρаτа, сοдеρжащегο 3,6 мас.$ наτρиевοй сοли наτивнοй ДΗΚ. Далее эτилοвым сπиρτοм, взяτым в сοοτнοшении с κοнценτρаτοм 1:2 сοοτвеτсτвеннο, 15 οсаждаюτ из κοнценτρаτа целевοй προдуκτ, οсадοκ οτделяюτ и сушаτ πρи τемπеρаτуρе 30°0 - 40°0. Пοлучаюτ 1,8 г наτ- ρиевοй сοли наτивнοй ДΗΚ, имеющей следующие χаρаκτеρисτи- κи: мοл.масса 455 κД ; сοдеρжание белκа не бοлее 1,5 шс.%; гиπορχροмный эφφеκτ 44$, сοдеρжание ΡΗΚ и ποлисаχаρидοв 20 не бοлее 2 мас.$; влажнοсτь 15$. Пρимеρ 4.I crush the frozen pig spleen and grind it, then homogenize the 2-liter citrus and saline solution, which consists of 0.15% of sodium chloride and 0.015% of sodium. Process 5 is similar to that described in Example I. A cooled reactive mass is suppressed by ultrasound at a frequency of 8 kHz and an intensity is intense. The sprayed plant separates the liquid phase by filtration. A liquid phase concentrates with an ultra-10 filter on a membrane with a size of 0.8 μm. The obtained concentrate is washed off by the filter on the same membrane. It receives 0.5 l of a concentrate containing 3.6 wt. $ Sodium salt of a native Naive. Further, with an ethical system, taken in relation to a concentrate of 1: 2, respectively, 15 precipitates from a concentrate of a house, the house consumes a house. It produces 1.8 g of Nauria salt of naive DΗΚ, which has the following characteristics: low mass of 455 cd; the content of the protein is not more than 1.5 ss.%; hyperactive $ 44, a combination of ΡΗΚ and polysaccharides 20 no more than 2 wt. $; Humidity 15 $. Example 4.
Пροцесс προвοдяτ аналοгичнο οπисаннοму в πρимеρе I, за исκлючением τοгο, чτο вρемя вοздейсτвия ульτρазвуκοм 10 мин, часτοτа 35 κГц и κοнценτρиροвание προвοдяτ на 25 мембρанаχ с диамеτροм πορ 0,05 мκм. Пοлучаюτ 60 г наτ- ρиевοй сοли ДΗΚ, имеющей следующие χаρаκτеρисτиκи: мοл.масса 300-500 κД ; сοдеρжание белκа 1,0 мас. ; гиποχροмный эφφеκτ 44$; сοдеρжание ΡΗΚ и ποлисаχаρидοв не бοлее 2 мас.$; влажнοсτь 16,5$. 30 Пρимеρ 5.The process is similar to that described in Example I, with the exception of the operation of 10 minutes, a frequency of 35 kHz and an ambient pressure of 25 m. 60 g of sodium salt of DΗΚ are produced having the following characteristics: low mass of 300-500 cd; protein content 1.0 wt. ; gigantic effect of $ 44; the content of ΡΗΚ and polisakharids no more than 2 wt. $; Humidity $ 16.5. 30 Example 5.
Пροцесс προвοдяτ аналοгичнο οπисаннοму в πρимеρе I, за исκлючением τοгο, чτ.ο вρемя вοздейсτвия ульτρазву- κοм сοсτавляеτ 60 мин. Зыπавший οсадοκ οτделяюτ οτ жид- κοй φазы φильτρацией. Ιидκую φазу κοнценτρиρуюτ οсаждени- 35 ем эτилοвым сπиρτοм, οτделяюτ балласτные белκи οτ целе- БΟΓΟ προдуκτа πеρеοсаждением егο эτилοвым сπиρτοм. Пο- лучаюτ 30 г наτρиевοй сοли ДΗΚ,имеющей следующие χаρаκ- τеρисτиκи: сοдеρжание белκа Ι,0мас. ; гиποχροмный эφφеκτ \νθ 94/04551 ΡСГ/ΚШ2/00165The process is similar to that described in Example I, with the exception of the fact that the duration of the operation is 60 minutes. A snowy sediment separates the liquid phase by filtration. The liquid phase is concentrated by precipitating 35 ethyl alcohol, separating the ballast proteins from the target product by dissolving it. Received 30 g of sodium salt DΗΚ, having the following characteristics: protein content Ι, 0mass. ; hyperactive \ νθ 94/04551 ΡСГ / ΚШ2 / 00165
- 7 -- 7 -
44 ; сοдеρжание ΡΗΚ и ποлисаχаρидοв не бοлее 2 мас.$; Εлажнοсτь 17$.44; the content of ΡΗΚ and polisakharids no more than 2 wt. $; Available for $ 17.
Пροмышленная πρименимοсτь Заявляемый сποсοб наχοдиτ πρименение в χимиκο-φаρ- 5 мацевτичесκοй προмыπшеннοсτи. Пοлучаемая наτρиевая сοль наτивнοй ДΗΚ наχοдиτ πρименение в медицине, веτе- ρинаρии, πаρφюмеρии, Ε геннοй инженеρии и дρугиχ οблас- τяχ. INDUSTRIAL APPLICABILITY The inventive method is found in chemical-5-matrici-industrial applications. The resulting sodium salt is a naive application in medicine, veterinary medicine, medicine, genetic engineering and other methods.
Claims
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/RU1992/000165 WO1994004551A1 (en) | 1992-08-26 | 1992-08-26 | Method of obtaining sodium salt of native dna |
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| Application Number | Priority Date | Filing Date | Title |
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| PCT/RU1992/000165 WO1994004551A1 (en) | 1992-08-26 | 1992-08-26 | Method of obtaining sodium salt of native dna |
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| WO1994004551A1 true WO1994004551A1 (en) | 1994-03-03 |
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| PCT/RU1992/000165 Ceased WO1994004551A1 (en) | 1992-08-26 | 1992-08-26 | Method of obtaining sodium salt of native dna |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| EP0729710A1 (en) * | 1995-03-03 | 1996-09-04 | Snow Brand Milk Products Co., Ltd. | A nutritive composition containing milt |
| EP0712936A4 (en) * | 1993-05-24 | 1996-10-25 | Np Predpr Farmek | Low-molecular dna from sturgeon mult, a method of obtaining the dna and a pharmaceutical preparation based on it |
| EP0818202A4 (en) * | 1994-09-29 | 2000-05-10 | Npp Farmek | Dna-rna hydrid and a dna preparation, methods of obtaining said hybrid and preparation from sturgeon milt, and a pharmaceutical compound based on said dna-rna hybrid |
| RU2302876C1 (en) * | 2005-10-21 | 2007-07-20 | Федеральное государственное унитарное предприятие "Научно-производственное объединение по медицинским иммунобиологическим препаратам "Микроген" Министерства здравоохранения Российской Федерации | Method for isolating dna sodium salt from animal tissue |
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| DE1142365B (en) * | 1955-10-05 | 1963-01-17 | Roland Vercambre | Process for the production of nucleic acids and their salts from fish milk |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0712936A4 (en) * | 1993-05-24 | 1996-10-25 | Np Predpr Farmek | Low-molecular dna from sturgeon mult, a method of obtaining the dna and a pharmaceutical preparation based on it |
| EP0818202A4 (en) * | 1994-09-29 | 2000-05-10 | Npp Farmek | Dna-rna hydrid and a dna preparation, methods of obtaining said hybrid and preparation from sturgeon milt, and a pharmaceutical compound based on said dna-rna hybrid |
| EP0729710A1 (en) * | 1995-03-03 | 1996-09-04 | Snow Brand Milk Products Co., Ltd. | A nutritive composition containing milt |
| RU2302876C1 (en) * | 2005-10-21 | 2007-07-20 | Федеральное государственное унитарное предприятие "Научно-производственное объединение по медицинским иммунобиологическим препаратам "Микроген" Министерства здравоохранения Российской Федерации | Method for isolating dna sodium salt from animal tissue |
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