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WO1994002833A1 - Cuvette et cartouche de cuvette pour un analyseur chimique - Google Patents

Cuvette et cartouche de cuvette pour un analyseur chimique Download PDF

Info

Publication number
WO1994002833A1
WO1994002833A1 PCT/US1993/006069 US9306069W WO9402833A1 WO 1994002833 A1 WO1994002833 A1 WO 1994002833A1 US 9306069 W US9306069 W US 9306069W WO 9402833 A1 WO9402833 A1 WO 9402833A1
Authority
WO
WIPO (PCT)
Prior art keywords
cuvette
cuvettes
cartridge
edges
side walls
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1993/006069
Other languages
English (en)
Inventor
Norman Kelln
Thomas Tiffany
Robin Olson
Bruce Weyrauch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alfa Wassermann Inc
Spectrum Systems Inc
Original Assignee
Alfa Wassermann Inc
Spectrum Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alfa Wassermann Inc, Spectrum Systems Inc filed Critical Alfa Wassermann Inc
Priority to EP93919806A priority Critical patent/EP0650590B1/fr
Priority to AU49914/93A priority patent/AU4991493A/en
Priority to DE69310515T priority patent/DE69310515T2/de
Publication of WO1994002833A1 publication Critical patent/WO1994002833A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00495—Centrifuges
    • G01N2035/00504—Centrifuges combined with carousels

Definitions

  • This disclosure pertains to a cuvette designed for use in a clinical chemistry analyzer for the testing of patient samples, such as blood or urine.
  • Automated analyzers have been developed for biochemical analysis of patient samples, such as whole blood, serum, urine, plasma and cerebral spinal fluid. Such analyzers carry out tests for assays in defined or random sequences of operations designed for efficient, high volume test procedures. They typically involve use of a reaction turntable that holds mixtures of reagents and samples within receiving cuvettes. Liquid materials are inserted into the cuvettes from a movable pipette. They are mixed and centrifuged for optical analytical test purposes. Much of such equipment is geared to very high volume test procedures.
  • the cuvettes are generally multi-compartment elements that partially or completely meet the requirements of a turntable. Many of these cuvettes are reusable.
  • the present cuvette is designed for use as a disposable item, each cuvette handling a single mixture of sample and reagent for analytical purposes.
  • the cuvettes are designed for use in an angularly inclined position on a complementary turntable, which facilitates insertion of new cuvettes from the center of the turntable and ejection of spent cuvettes from its periphery. Details of the invention will be understood from the accompanying drawings and text. Brief Description of the Drawings
  • Fig. 1 is a side elevation view of a cuvette
  • Fig. 2 is a top view
  • Fig. 3 is a sectional elevation view taken along line 3-3 in Fig. 2;
  • Fig. 4 is a front elevation view of a loaded cuvette cartridge;
  • Fig. 5 is a transverse sectional view through a loaded cartridge as seen along line 5-5 in Fig. 4;
  • Fig. 6 is a plan view of the cuvette turntable;
  • Fig. 7 is an enlarged sectional view taken along line 7-7 in Fig. 6. Best Modes for Carrying Out the Invention and Disclosure of Invention
  • the automatic chemical analyzer for which this cuvette was designed includes a turntable 11 rotatably mounted about a vertical axis.
  • a plurality of disposable cuvettes 10 are releasably mounted to the turntable 11.
  • a motor is operably connected to turntable 11 for alternately (1) indexing it at a stationary angular position about its axis with a selected cuvette 10 positioned at a cuvette access station or (2) turning it about its axis while mixing or centrifuging the contents of cuvettes mounted to it.
  • An analytical means such as an optical system, is provided adjacent to the turntable 11 for performing tests on the contents of the cuvettes 10 as they rotate about the turntable axis.
  • the analyzer also includes a vertical probe arm (not shown) movable about a vertical axis.
  • the probe arm supports a downwardly-extending open pipette for movement along an arcuate path intersecting both the cuvette access station and a container access station where regents and samples are supplied to the open end of the pipette. It can move along the arcuate path in a random fashion to transfer liquid from a container positioned at the container access station to a cuvette 10 positioned on the turntable 11 at the cuvette access station.
  • the disposable cuvettes 10 designed for use in turntable 11 are illustrated in detail in Figs. 1-3.
  • a complementary cartridge 40 for handling and storing the cuvettes is shown in Figs. 4 and 5.
  • Cuvettes 10 are molded from a suitable transparent rigid plastic material that is liquid impervious and inert to the liquids which they are to contain.
  • the cross-sectional configuration of each cuvette is rectangular.
  • Each cuvette 10 includes two identical side walls 45 having parallel top and bottom edges.
  • the straight top and bottom edges along each side wall 45 longitudinally overlap one another to facilitate stacking of the cuvettes in abutting parallel positions.
  • Side walls 45 are transversely joined by parallel spaced top and bottom walls 46 and 47.
  • each cuvette 10 termed its “upper end” includes an opening 52 between the end edges of the side walls 45. Opening 52 provides access to the interior of cuvette 10 for receipt of liquids.
  • the edges of side walls 45 that form the opening 52 include angular edges intersecting the straight top edges of the respective side walls 45. The angular edges assume horizontal orientations when positioned in turntable 11 (Fig. 7).
  • the end edges along the opening 52 are perpendicular to the top and bottom walls 46 and 47.
  • the opening of cuvettes that are pre-loaded with reagents or other materials before use in the chemistry instrument 24 can optionally be sealed by a suitable film or other cover (not shown) capable of being pierced by the descending tip of pipette 18.
  • each cuvette 10 includes perpendicular optical surfaces for transmission of light in conjunction with operation of the optical system 14. These surfaces include top, end and bottom optical surfaces 48, 49 and 50, respectively. Each optical surface area is slightly recessed inwardly from the outer edges of side walls 45 to protect the optical surfaces from abrasion or contact during handling.
  • each cuvette 10 has a transverse protruding ledge 68 extending across the two side walls 45.
  • Ledge 68 provides a continuous transverse surface for abutment of the upper end of an adjacent cuvette 10 when one cuvette pushes another into position within turntable 11.
  • a small downwardly-facing recess 51 is provided within the bottom wall 47 of each cuvette 10 next to the inner end of lower optical surface 50.
  • the recess 51 serves as a detent in conjunction with a spring-biased enlargement on a spring member 55 (see Fig. 7) that yieldably holds cuvette 10 within a receiving compartment on the turntable 11.
  • the cuvettes are preferably packaged within elongated disposable cartridges 40 (see Figs. 4 and 5).
  • the parallel stacked cuvettes 10 face oppositely at the respective ends of cartridge 40.
  • the cartridge 40 is designed for insertion into an open slot of a receiving cuvette magazine (not shown).
  • the cuvette cartridge 40 is formed from a C-shaped channel 41 having interior surfaces complementary to the exterior shape and size of the individual cuvettes 10.
  • Cartridge 40 can be formed from any suitable stiff, resilient plastic sheet or can be extruded in the shape illustrated in Figs. 4 and 5. Its purpose is to facilitate handling and storage of the large quantities of cuvettes 10 required by each chemistry instrument. It expedites manual entry of cuvettes 10 into a storage magazine included within the instrument.
  • Cuvettes 10 fit transversely within the elongated channel 41 in abutting parallel positions within two groups.
  • Each group of cuvettes 10 at the respective ends of cartridge 40 can be identical to a full stack of cuvettes within the receiving magazine.
  • Two pairs of inwardly bent stops 42 near the center of cartridge 40 limit inward motion of cuvettes along the length of the magazine. Outward movement of cuvettes at each end of the cartridge 40 is resisted by smaller end stops 43.
  • the stops 43 are bent inwardly to partially intersect the path of cuvettes 10 as they exit the cartridge 40.
  • Turntable 11 is detailed in Figs. 6 and 7. It includes a circular, radially- slotted wheel adapted to be rotatably mounted about a vertical axis.
  • the outer periphery of the turntable 11 presents a series of equiangularly spaced radial compartments 53.
  • Each compartment 53 individually receives a cuvette 10 in the manner shown in Fig. 7.
  • Each compartment 53 includes a radial slot having an interior cross-sectional shape and size that complements the exterior cross-sectional shape and size of a cuvette 10.
  • the compartments 53 are arranged about turntable 11 at an angle such that the angular upper edge of each cuvette opening will be oriented horizontally.
  • the angular upper edge is also perpendicular to the turntable axis.
  • the angular nature of each compartment also positions the optical end of each cuvette 10 at a lower elevation than opening 52, assuring that liquids in each cuvette will not spill, even while turntable 11 is stationary.
  • An axial slot 54 intersects each compartment 53 across its outer end. Slots 54 extend through the upper and lower surfaces of the turntable 11. They provide light access to the optical surfaces 48-50 of cuvettes 10. Slots 54 permit passage of light through the individual cuvettes 10. They are used in conjunction with operation of an optical system (not shown) to facilitate photometric testing of cuvette contents while within the turntable 11.
  • the cuvettes are yieldably held within the radial compartments 53 by spring 55 (Fig. 7).
  • the outer ends 61 of the longitudinal springs 55 are enlarged to enable them to fit within the recesses 51 formed in the cuvettes 10.
  • the spring ends 61 radially position each cuvette 10 within the respective turntable compartments 53. Springs 55 also serve as interior supports within compartments 53.
  • the cover 59 about turntable 11 is fabricated of an electrically conductive plastic material. Springs 55 maintain firm surface-to-surface contact between the top wall 46 of each cuvette 10 and the interior surface of cover 59. This provides effective heat transfer to each cuvette to reduce the time required to warm it in preparation for receipt of a test sample.
  • the cover 59 can be heated as the turntable 11 is rotated, using an adjacent stationary source of controlled heat (not shown).
  • Cuvettes 10 held within the turntable 11 are individually accessible and remain open for reception of samples and reagents as required by requisitioned assays. Liquids can be introduced through the openings 52 of the respective cuvettes 10 by operation of a movable probe arm and pipette (not shown) at a cuvette access station. All incubation of samples involved in an assay can occur within cuvettes 10 while positioned within turntable 11.
  • the upper surface of the supporting central plate 56 on turntable 11 has a plurality of tapered radial guide surfaces 57 formed about it. Surfaces 57 are centered between each radial compartment 53 and are obliquely aligned with the respective compartments 53. They are used to index turntable 11 during reception of incoming cuvettes.
  • the turntable 11 holds cuvettes 10 in elevationally tilted radial positions within the individual receptacles 53. Their openings 52 of the cuvettes 10 are exposed in the turntable 11 for reception of liquid materials. Their optical surfaces 48, 49 and 50 are exposed through slots 54 at the periphery of turntable 11 for transmission of light as required by operation of the related optical system.
  • the combination of the angular orientation of the cuvettes 10 within the receptacles 53 of turntable 11 and the provision of the elongated opening 52 across the upper end of each cuvette 10 provides an open access area to the interiors of the installed cuvettes 10 that does not permit splashing of fluids as they are delivered through a vertical pipette.
  • the inclined angular positioning of cuvettes 10 within the individual receptacles 53 in the turntable 11 also prevents splashing or loss of fluids from within the cuvettes 10 during mixing operations that can be carried out by operation of the turntable 11.
  • Extending the cuvette opening 52 along one side of each cuvette is an improvement specifically adapted to the angular cuvette orientation utilized in the illustrated turntable 11.
  • the inclined angular orientation of the individual cuvettes 10 within the receiving turntable shown in Figs. 6 and 7 allows each cuvette to be installed by pushing it radially outward from the center of the turntable 11 in a "feedthrough" arrangement, as indicated by arrow 60.
  • the preceding cuvette will be serially pushed from the turntable 11, as indicated by arrow 62.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Optical Measuring Cells (AREA)

Abstract

Une cuvette jetable (10) est moulée en une résine plastique transparente et rigide qui est imperméable aux liquides qu'elle est destinée à contenir. La cuvette (10) présente une section transversale rectangulaire. Des parois latérales identiques (45) s'étendent entre des extrémités supérieure et inférieure de chaque cuvette, l'extrémité supérieure étant ouverte de manière à permettre la réception des liquides et l'extrémité inférieure étant fermée. Des zones de surface optique en retrait (48, 50) à l'extrémité inférieure sont perpendiculaires l'une à l'autre et permettent la vérification optique du contenu de la cuvette. Une cartouche allongée (40) contient une pile de cuvettes adjacentes (10) de manière à en faciliter la manipulation.
PCT/US1993/006069 1992-07-16 1993-06-24 Cuvette et cartouche de cuvette pour un analyseur chimique Ceased WO1994002833A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP93919806A EP0650590B1 (fr) 1992-07-16 1993-06-24 Cuvette et cartouche de cuvette pour un analyseur chimique
AU49914/93A AU4991493A (en) 1992-07-16 1993-06-24 Cuvette and cuvette cartridge for a chemical analyzer
DE69310515T DE69310515T2 (de) 1992-07-16 1993-06-24 REAKTIONSKüVETTE UND KASSETTE FUER EINEN CHEMISCHEN ANALYSATOR

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/916,040 1992-07-16
US07/916,040 US5292484A (en) 1992-07-16 1992-07-16 Cuvette and cuvette cartridge for a chemical analyzer

Publications (1)

Publication Number Publication Date
WO1994002833A1 true WO1994002833A1 (fr) 1994-02-03

Family

ID=25436615

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1993/006069 Ceased WO1994002833A1 (fr) 1992-07-16 1993-06-24 Cuvette et cartouche de cuvette pour un analyseur chimique

Country Status (6)

Country Link
US (1) US5292484A (fr)
EP (1) EP0650590B1 (fr)
AU (1) AU4991493A (fr)
DE (1) DE69310515T2 (fr)
ES (1) ES2103086T3 (fr)
WO (1) WO1994002833A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1949962A1 (fr) * 2007-01-25 2008-07-30 F.Hoffmann-La Roche Ag Dispositif et procédé pour séparer un composant liquide d'un échantillon sanguin et appareil d'analyse comprenant ledit dispositif

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GB2319836B (en) * 1996-11-25 2001-04-04 Porvair Plc Microplates
US6632654B1 (en) * 2001-08-08 2003-10-14 Dade Microscan Inc. Canister for inventorying susceptability test devices in an automated microbiological analyzer
DE10200499A1 (de) * 2002-01-03 2003-07-10 Zeiss Carl Jena Gmbh Verfahren und/oder Anordnung zur Identifikation von fluoreszierenden, lumineszierenden und/oder absorbierenden Substanzen auf und/oder in Probenträgern
US7402281B2 (en) * 2003-07-18 2008-07-22 Siemens Healthcare Diagnostics Inc. Magazine for inventorying reaction cuvettes in an automatic analyzer
EP1516920A1 (fr) * 2003-09-19 2005-03-23 The Automation Partnership Récipient de culture de cellules pour la culture automatisée de cultures de cellules
US20100145331A1 (en) * 2004-06-02 2010-06-10 Chrisitian Steven C Loop Ablation Apparatus and Method
USD533947S1 (en) 2005-05-04 2006-12-19 Abbott Laboratories Reagent carrier for use in an automated analyzer
US7628954B2 (en) 2005-05-04 2009-12-08 Abbott Laboratories, Inc. Reagent and sample handling device for automatic testing system
USD531736S1 (en) 2005-05-04 2006-11-07 Abbott Laboratories Reagent carrier for use in an automated analyzer
USD534280S1 (en) 2005-05-04 2006-12-26 Abbott Laboratories Reagent carrier for use in an automated analyzer
USD532524S1 (en) 2005-05-04 2006-11-21 Abbott Laboratories Reagent carrier for use in an automated analyzer
US20080084559A1 (en) * 2006-10-10 2008-04-10 C Technologies, Inc. Microvolume sampling device
US7723613B2 (en) * 2007-11-30 2010-05-25 Motorola, Inc. Angled double-wall junction
US9619627B2 (en) 2011-09-25 2017-04-11 Theranos, Inc. Systems and methods for collecting and transmitting assay results
ES2700498T3 (es) 2012-07-25 2019-02-18 Theranos Ip Co Llc Sistema para el análisis de una muestra
US9513224B2 (en) 2013-02-18 2016-12-06 Theranos, Inc. Image analysis and measurement of biological samples
US9176154B2 (en) 2012-12-12 2015-11-03 Bio-Rad Laboratories, Inc. Calibration process and system
EP3001177A1 (fr) * 2014-09-29 2016-03-30 Grundfos Holding A/S Dispositif destiné à détecter des particules dans un liquide
USD808036S1 (en) 2015-09-29 2018-01-16 Bd Kiestra B.V. Cuvette
USD810959S1 (en) 2015-09-29 2018-02-20 Bd Kiestra B.V. Cuvette tray
US10768105B1 (en) 2016-07-29 2020-09-08 Labrador Diagnostics Llc Image analysis and measurement of biological samples
CN115356180B (zh) * 2022-10-24 2023-01-03 莱阳市检验检测中心 一种食品安全检测仪

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GB1357232A (en) * 1959-11-20 1974-06-19 Bio Dynamics Inc Centrifuge clinical chemistry analysis
GB2049930A (en) * 1979-05-10 1980-12-31 Hoffmann La Roche Multi-cell centrifugal analyser ring
DE3104796A1 (de) * 1981-02-11 1982-09-09 Eppendorf Gerätebau Netheler + Hinz GmbH, 2000 Hamburg Palette mit in zeilen und spalten angeordneten kuevetten

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GB1357232A (en) * 1959-11-20 1974-06-19 Bio Dynamics Inc Centrifuge clinical chemistry analysis
US3718439A (en) * 1970-06-12 1973-02-27 Instrumentation Labor Inc Analytical apparatus
GB2049930A (en) * 1979-05-10 1980-12-31 Hoffmann La Roche Multi-cell centrifugal analyser ring
DE3104796A1 (de) * 1981-02-11 1982-09-09 Eppendorf Gerätebau Netheler + Hinz GmbH, 2000 Hamburg Palette mit in zeilen und spalten angeordneten kuevetten

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1949962A1 (fr) * 2007-01-25 2008-07-30 F.Hoffmann-La Roche Ag Dispositif et procédé pour séparer un composant liquide d'un échantillon sanguin et appareil d'analyse comprenant ledit dispositif

Also Published As

Publication number Publication date
DE69310515T2 (de) 1997-11-13
US5292484A (en) 1994-03-08
AU4991493A (en) 1994-02-14
EP0650590B1 (fr) 1997-05-07
EP0650590A1 (fr) 1995-05-03
DE69310515D1 (de) 1997-06-12
ES2103086T3 (es) 1997-08-16

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