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WO1993002210A1 - Dispositif et procedes de conservation, transport, stockage, rehydratation et administration de micro-organismes viables - Google Patents

Dispositif et procedes de conservation, transport, stockage, rehydratation et administration de micro-organismes viables Download PDF

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Publication number
WO1993002210A1
WO1993002210A1 PCT/US1992/006135 US9206135W WO9302210A1 WO 1993002210 A1 WO1993002210 A1 WO 1993002210A1 US 9206135 W US9206135 W US 9206135W WO 9302210 A1 WO9302210 A1 WO 9302210A1
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WIPO (PCT)
Prior art keywords
organisms
cap
vial
dried
kit
Prior art date
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Ceased
Application number
PCT/US1992/006135
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English (en)
Inventor
Gerald L. Chrisope
Nell C. Roberts
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Chrisope Technologies Inc
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Chrisope Technologies Inc
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Filing date
Publication date
Application filed by Chrisope Technologies Inc filed Critical Chrisope Technologies Inc
Publication of WO1993002210A1 publication Critical patent/WO1993002210A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B47/00—Pumps or pumping installations specially adapted for raising fluids from great depths, e.g. well pumps
    • F04B47/02—Pumps or pumping installations specially adapted for raising fluids from great depths, e.g. well pumps the driving mechanisms being situated at ground level
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/03—Means for pre-treatment of biological substances by control of the humidity or content of liquids; Drying
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/22—Means for packing or storing viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • E—FIXED CONSTRUCTIONS
    • E21—EARTH OR ROCK DRILLING; MINING
    • E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
    • E21B47/00—Survey of boreholes or wells
    • E21B47/007—Measuring stresses in a pipe string or casing
    • E—FIXED CONSTRUCTIONS
    • E21—EARTH OR ROCK DRILLING; MINING
    • E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
    • E21B47/00—Survey of boreholes or wells
    • E21B47/008—Monitoring of down-hole pump systems, e.g. for the detection of "pumped-off" conditions
    • E21B47/009—Monitoring of walking-beam pump systems
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B49/00—Control, e.g. of pump delivery, or pump pressure of, or safety measures for, machines, pumps, or pumping installations, not otherwise provided for, or of interest apart from, groups F04B1/00 - F04B47/00
    • F04B49/06—Control using electricity
    • F04B49/065—Control using electricity and making use of computers

Definitions

  • the present invention generally relates to a system and method for preserving, transporting, storing, re-hydrating and delivering viable micro ⁇ organisms. More particularly, the present invention is directed to a kit and method for preserving and storing dried, microbiological organisms and for re-hydrating and delivering specific and reproducible numbers of viable organisms, most preferably as a single, total dose of cells.
  • the quantitative determination of the number of pathogenic and/or indicator micro-organisms in a sample is as important in many public health applications as the mere determination of the presence or absence of those organisms.
  • the infective dose i.e., the number of micro ⁇ organisms required to produce infection in the host, has been determined for many species. Although strain dependent, the infective dose may vary from as few as ten organisms of Shigella ⁇ ysenteriae to as many as one hundred million or more for Salmonella typhosa or Vibrio cholerae.
  • the wholesomeness or sanitary quality of food, milk, other dairy products, shellfish, potable water, shellfish growing waters, waste waters and a variety of surface waters is determined by the quantitative enumeration of specific indicator organisms.
  • the federal Food and Drug Administration has equally stringent requirements and regulations controlling the microbial purity, i.e., sterility and effectiveness, of microbial preservatives in food, drugs, and cosmetics for human consumption and/or use.
  • FDA Food and Drug Administration
  • the Centers for Disease Control (CDC) in Atlanta, Georgia require both inhibition of quantitative challenge doses of saprophytic micro-organisms and recovery of quantitative doses of Neisseria gonorrhoeae and Neisseria meningitis by highly selective culture media such as Thayer Martin Agar and its modifications.
  • the vial served as a reservoir and the system depended on the accuracy of the individual user to measure the rehydrating fluid, to properly mix the lyophilized culture and rehydrating fluid and to withdraw the defined aliquots.
  • this method suffered from many opportunities for contamination of both the environment and the user resulting from the use of various needles, syringes or pipettes. Because the foregoing sources have been unacceptable, the current state of the art for the aforementioned procedures requiring specific numbers of control micro-organisms has required on-site preparation of estimated doses of living micro-organisms.
  • the present invention is directed to systems and methods for preserving, transporting, storing, re-hydrating and delivering specific and reproducible numbers of viable micro-organisms, most preferably as a single, total dose of cells. More particularly, the system is directed to a kit having a first vial and cap combination and including on fixative sites on the underside of the cap dried microbiological organisms.
  • the vial both contains and is surrounded by a dry, biologically inert atmosphere, typically merely an oxygen-free atmosphere.
  • the vial and cap combination is prepared by disposing on the fixative sites a liquid containing the microbiological organisms, drying that liquid under mild conditions, i.e., ambient temperature and pressure preferably using a forced air flow, to aid survivability of the micro ⁇ organisms and sealing the cap to a vial previously purged of oxygen or any other biologically undesirable gas and containing a desiccant.
  • mild conditions i.e., ambient temperature and pressure preferably using a forced air flow
  • the vial further includes a barrier to prevent contact of the desiccant with the dried micro-organisms.
  • this first vial and cap combination including the dried microbiological organisms, is sealed within a second packaging material in a dry, biologically inert atmosphere, preferably a metallic foil or plastic film, e.g., conventional mylar packaging material.
  • the kits of the presently preferred embodiment further include a second vial and cap combination having disposed therein a pre-measured quantity of a liquid suitable for re-hydrating the dried micro-biological organisms disposed on the first cap.
  • the vials and caps of the kit are constructed so that the caps are interchangeable.
  • the dried organisms may be easily re-hydrated by transferring the cap containing those organisms to the vial containing the re-hydrating fluid and inverting to bring the fluid into contact with the dried organisms.
  • these steps comprise the method of the present invention for re-hydrating dried microbiological organisms preserved and stored in accord with the previously described method. Once rehydrated, the organisms may be delivered as a single, total dose of cells.
  • the fixative sites of the first cap When a known quantity of viable micro-organisms is disposed on the fixative sites of the first cap, dried using the specified mild conditions and sealed in a dry, biologically inert atmosphere, the resulting dried organisms may be transported and stored for extended periods at normal refrigeration temperatures. More importantly, because of the mild processing and storage conditions, a specific and reproducible number of viable micro-organisms may be delivered for use in subsequent tests.
  • kits of the present invention permit the preservation and storage for extended periods at normal refrigeration temperatures of microbiological organisms which previously were preserved by drying in harsh conditions, e.g., lyophilization, and could not be stored for extended periods except at extremely low temperatures, e.g., less than -20°C.
  • FIG. 1 is a side cross-sectional illustration of a first vial and cap combination including fixative sites with dried micro-organisms affixed thereto in a dry, biologically inert atmosphere in accord with the present invention
  • Fig. 2 is an elevation looking into the bottom of the cap of Fig. 1 illustrating the underside of the cap having fixative sites with dried micro ⁇ organisms affixed thereto in accord with the present invention
  • Fig. 3 is a side cross-sectional illustration of a second vial and cap combination in accord with the present invention and including therein a pre-determined quantity of a re-hydrating liquid
  • Fig. 4 is an elevational illustration of a kit in accord with the present invention including a first vial containing dried microbiological organisms and a second vial containing a pre-measured quantity of re ⁇ hydrating fluid, both disposed in separate, sealed compartments of a mylar packaging pouch.
  • the kit 100 of the present invention comprises a first vial 10 having therein dried microbiological organisms 40 and a second vial 110 having therein a pre-measured quantity of re-hydrating fluid 140.
  • First vial 10 and second vial 110 are illustrated, respectively, in Figs. 1 and 3, while the kit 100 of the present invention is illustrated in Fig. 4.
  • a first, sealable vial and cap combination 10 comprises a vial 50 together with a cooperating cap 20.
  • Cap 20 is sealingly engageable with vial 50 by any conventional construction.
  • vial 50 and cap 20 are constructed from a conventional, preferably hard, plastic.
  • any appropriate vial and cap material such as glass, metal or the like may be employed.
  • vial 50 is comprised of a sterile plastic, e.g., polyethylene, polypropylene or another polymeric material.
  • Vial 50 is provided with threads 52 about its exterior open end.
  • the underside of cap 20 includes about the periphery thereof a rim 22 with interior facing threads 26 for engagement with threads 52 of vial 50.
  • the underside of cap 20 is further characterized by protruding, circular lip 28.
  • Lip 28, together with rim 22 define a groove 32 for receiving an elastomeric 0-ring, washer or other sealing device 30 for cooperation with the end of vial 50 to ensure sealing engagement between vial 50 and cap 20.
  • Lip 28 also defines on the interior thereof an area having a plurality of fixative sites 24, preferably irregularities to which are adhered microbiological organisms such as those illustrated in film 40.
  • Fixative sites 24 may be formed on the interior surface of cap 20 by abrading, scoring, scratching, or otherwise marring the surface with any appropriate instrument or means to produce an irregular, unsmooth surface to aid adhesion of the dried microbiological organism film 40.
  • One convenient method of forming acceptable irregularities includes the scoring of surface 24 with an electrical drill bit.
  • at least the underside of cap 20 is comprised of a plastic material, pre-molded with irregular, rough fixative sites 24.
  • the film 40 of dried microbiological organisms may be produced by disposing a known volume of liquid containing a known quantity of viable organisms onto the inverted cap 20 within the circumscribed area of fixative sites 24 surrounded by retaining lip 28. Subsequent evaporation and drying produces the illustrated film 40 of microbiological organisms. Preferred methods are described in more detail below.
  • Vial 50 preferably further includes a conventional desiccant, e.g. , tablet 58, together with a sterile barrier, e.g., cotton insert 56, to prevent
  • the interior 36 of the sealed vial 50 and cap 20 combination 10 is provided with a dry, biologically inert atmosphere, preferably an oxygen-free atmosphere.
  • a dry, biologically inert atmosphere preferably an oxygen-free atmosphere.
  • This atmosphere aids in preservation of viable, dried organisms.
  • Those skilled in the art are aware of many methods for producing such an atmosphere. While creation of a vacuum might be acceptable, the preferred methods of the present invention include flushing of the interior 36 of vial 50 with a biologically inert gas and sealing with an atmosphere of the flushing gas. While any biologically inert gas may be used, the preferred gases include nitrogen and the noble gases, i.e., helium, argon, neon and the like.
  • vial 50 containing a film 40 of dried organisms is, itself, sealed in a biologically inert atmosphere of the type previously described in a second packaging 104, preferably a non- breakable packaging such as a mylar or metallic film pouch 102.
  • Vial and cap combination 110 for holding a pfe-measured quantity of re-hydrating fluid 140 is illustrated in Fig. 3.
  • Combination 110 includes a vial 150 substantially identical to vial 50 in all respects and sealingly engageable both with cap 120 and with cap 20.
  • Vial 150 includes grip ring 154 and exterior facing threads 152 about its open end.
  • Cap 120 is substantially similar to cap 20 with the exception that the central portion of the underside of cap 120 may be smooth because there is no need for fixative sites 24.
  • Cap 120 is characterized by upset rim 122 about the periphery thereof with threads 126 on its interior surface for cooperation with threads 152 of vial 150.
  • Cap 120 further includes upset lip 128 to form groove 132 with the lower portion of rim 122 for receiving a conventional sealing means, e.g., an elastomeric O-ring, conventional elastomeric washer or other seal means 130.
  • a conventional sealing means e.g., an elastomeric O-ring, conventional elastomeric washer or other seal means 130.
  • the type and quantity of re-hydrating fluid 140 is chosen for compatibility with the dried micro-organisms in film 40 on cap 20 of the associated first vial and cap combination 10. Those skilled in the art are knowledgeable of the type and quantity of re-hydrating liquid to be used for any given organism.
  • second vial and cap combination 110 containing re-hydrating fluid 140 is disposed in a second portion 106 of pouch 102.
  • pouch 102 has been sealed at 108 to provide a first pouch portion 104 for receiving vial and cap combination 10 containing a film 40 of dried micro-organisms and a second pouch portion 106 for receiving vial and cap combination 110 containing the associated pre-measured quantity of re-hydrating fluid.
  • Cap 120 upon which has been disposed a film 40 of dried microbiological organisms is interchangeable with cap 120. Accordingly, rehydration of the dried organisms is easily achieved by transfer of cap 20 containing film 40 to vial 150 containing fluid 140 and inversion to bring the re-hydrating fluid 140 into contact with the dried micro-organisms.
  • a known quantity of micro-organisms have been dried onto fixative sites 24 in film 40 under mild conditions, i.e., ambient temperature and pressure and preferably using a simple forced air flow.
  • mild conditions i.e., ambient temperature and pressure
  • biologically active gases e.g., oxygen or carbon dioxide
  • reagent solutions typically comprise biologically acceptable additives or preservatives such as carbohydrates, proteins, reducing agents, toxin neutralizing agents, cryoprotective agents and the like.
  • micro-organism preservative reagent mixtures are dispensed in carefully measured amounts to the fixative sites 24 defined by lip 28 in the previously prepared and sterilized caps 20.
  • the precise number of viable micro-organisms may be determined by enumeration using standard micro-biological procedures which require a growth of the micro ⁇ organisms on appropriate culture media under standard conditions of temperature and atmosphere. During the dispensing step, a minimum of five aliquots representing the beginning, middle and end portions of the run should be selected at random for enumeration of colony forming units by standard procedures prior to the desiccation step.
  • the remaining micro-organism preservative suspensions are dried to a steady weight in a forced air desiccant chamber optionally containing silica gel or another desiccant material.
  • the forced air desiccant chamber should avoid extreme temperature conditions and should operate under mild, preferably ambient conditions, most preferably at a temperature from about 24 °C to about 32 °C. Drying times under the preferred conditions average from about 4 to about 5 hours.
  • Vial 50 is fitted with cotton plug 56 or another optional barrier material comprised of any suitable, biologically inert, porous material.
  • a desiccant preferably in the form of a molecular sieve caplet 58, or any other conventional, biologically acceptable desiccant, e.g., silica gel.
  • Vials 50 containing film 40 of dried microbiological organisms must be filled with a dry atmosphere substantially free of any gas used in the growth or decay of the chosen organisms, e.g., oxygen or carbon dioxide, to provide the best preservation and viability of the organisms.
  • a dry atmosphere substantially free of any gas used in the growth or decay of the chosen organisms, e.g., oxygen or carbon dioxide, to provide the best preservation and viability of the organisms.
  • Such an atmosphere may be referred to as a biologically inert atmosphere and the gases therein as biologically inert gases.
  • the vials are flushed with any dry, biologically inert gas. While the noble gases or mixtures thereof might be used, nitrogen is the preferred gas.
  • the vials might be evacuated to provide the required dry, biologically inert atmosphere.
  • the caps 20 containing the film 40 of dried microbiological organisms are then applied to and sealed to the vial 50 containing the dry, biologically inert atmosphere, and preferably a desiccant and sterile barrier to separate the desiccant from the film.
  • the assembled vial and cap combination 10 is then packaged and sealed in a non-breakable envelope 102, preferably in a first half 104 thereof which has also been provided with a dry, biologically inert atmosphere such as in the manner discussed above. Again, flushing with nitrogen gas is the preferred method.
  • pouch 104 may also contain an additional desiccant (not shown) to aid in maintaining the desired dry atmosphere.
  • re-hydrating fluids examples include Butterfield's phosphate buffer for rehydration of Enterobacte ⁇ aceae, Enterococcus, yeast and a variety of other non-fastidious Gram positive and Gram negative micro- organisms. More nutrient-containing formulas, e.g., trypticase soy broth, may be used for more fastidious organisms. Formulations such as thioglycollate broth may be used for anaerobic micro-organisms. Other suitable re-hydration fluids are well known to those skilled in the art for any variety of selected micro-organism. In order to quantitatively determine the number of viable micro ⁇ organisms available in the methods of the present invention, the following exemplary procedure may be employed.
  • a minimum of five (5) aliquots selected at random from the dried, assembled kits are analyzed immediately after preparation for viable colony forming units as previously discussed.
  • the cap containing the micro-organisms is screwed on and sealed to the re-hydrating fluid vial.
  • the newly assembled combination containing the micro-organisms and re-hydrating fluid is inverted to allow contact between the dried micro-organisms and the re-hydrating fluid.
  • the inverted combination is warmed for about ten (10) minutes at about 35°C to about 37°C to ensure complete solution of the dehydrated micro ⁇ organisms. No extra equipment or laboratory apparatus is required to re- hydrate and transfer the micro-organisms to culture media.
  • the produced suspension is ready for use in any procedure selected at the option of the analyst.
  • the entire contents may be decanted directly onto prepared agar medium for the spread plate method, into a sterile petri dish for the pour plate method, or into a dilution blank which may be examined by the membrane filter, multiple tube or most probable number method. While these and other tests are well known to those skilled in the art, detailed descriptions of some exemplary tests may be found in the literature.
  • Exemplary references include Standard Methods for the Examination of Water and Wastewater, 17th Edition (1989) and Standard Methods for Dairy Products, 15th Edition (1985) both published by the American Public Health Association and the Protocol and Producer Protocol for Testing Thayer Martin Media, published by the Centers for Disease Control.
  • Standard Methods for the Examination of Water and Wastewater 17th Edition (1989) and Standard Methods for Dairy Products, 15th Edition (1985) both published by the American Public Health Association and the Protocol and Producer Protocol for Testing Thayer Martin Media, published by the Centers for Disease Control.
  • very high numbers of living micro-organisms are added to the preservation formulation to compensate for the unpredictable loss of viability known to occur.
  • no significant change in viable colony counts during the dehydration process, storage at ordinary refrigeration temperatures, transport at ambient temperatures and re-hydration has been observed.
  • Escherichia coli has been used in a recently completed field trial experiment of the apparatus and method of the present invention.
  • Results of colony counts of five (5) aliquots analyzed by the conventional spread plate method prior to drying for this lot were 30, 34, 37, 43 and
  • kits in accord with the present invention.
  • kits were shipped at ambient temperatures to seven (7) different laboratories.
  • These laboratories re-hydrated the micro ⁇ organisms in accord with the methods of the present invention and analyzed the resulting solutions for viable organisms.
  • Each laboratory examined five (5) aliquots by the membrane filtration technique for coliforms utilizing m-endo agar incubated for 24 hours at 35 °C. Test results are reported in the following table.
  • Desirable reproducibility is achieved with a logarithmic variance equal to or less than 0.012, the accepted logarithmic variance set by the FDA for certified analysts performing standard plate counts on normal milk samples.
  • Six of the seven laboratories reported results having a variance between 0.001 and 0.007 with five of seven laboratories having variance less than or equal to 0.003. This test confirms the ability of the present apparatus and methods to deliver specific and reproducible numbers of viable organisms within acceptable statistical variance. While the exact shelf life of quantitated micro-organisms preserved and re-hydrated in accord with the present invention is the object of ongoing studies, no statistically significant change in viability or colony counts has been observed in at least the first four months of this study. Current shelf life studies are designed to follow single lots of different strains of micro-organisms for up to eighteen months or until a significant change occurs in the viable populations. No significant changes have yet been observed.

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Abstract

La présente invention se rapporte à des systèmes et des procédés de conservation, transport, stockage, réhydratation et administration de micro-organismes viables. L'invention se rapporte plus particulièrement à un coffret et à un procédé de conservation et de stockage d'organismes microbiologiques séchés consistant à réhydrater et administrer des quantités reproductibles et spécifiques de ces microorganismes viables. Le coffret et le procédé de la présente invention offrent la possibilité d'administrer une quantité connue de micro-organismes réhydratés sous forme d'une dose unique, complète de cellules, sans les imprécisions et les risques liés aux systèmes classiques à aiguille, à seringue ou de transfert par pipette. Le coffret comprend un premier et un second flacon et une combinaison de bouchons, le premier (10) contenant les organismes séchés (40) dans une atmosphère sèche, biologiquement inerte, et le second contenant une quantité prémesurée de fluide réhydratant (140). Une quantité définie d'organismes microbiologiques sélectionnés est séchée à des conditions modérées, par ex., à une température et une pression ambiantes utilisant de l'air pulsé pour s'écouler, vers des sites fixatifs sur la partie inférieure du premier bouchon (20) engagé hermétiquement dans le premier flacon (10). Le transfert de ce bouchon vers le second flacon contenant le fluide réhydratant permet une réhydratation facile et une capacité à administrer des quantités reproductibles et spécifiques d'organismes viables après un certain temps de stockage à des températures de réfrigération normales.
PCT/US1992/006135 1991-07-22 1992-07-21 Dispositif et procedes de conservation, transport, stockage, rehydratation et administration de micro-organismes viables Ceased WO1993002210A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US73369691A 1991-07-22 1991-07-22
US733,696 1991-07-22

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WO1993002210A1 true WO1993002210A1 (fr) 1993-02-04

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PCT/US1992/006135 Ceased WO1993002210A1 (fr) 1991-07-22 1992-07-21 Dispositif et procedes de conservation, transport, stockage, rehydratation et administration de micro-organismes viables
PCT/US1992/006105 Ceased WO1993002289A1 (fr) 1991-07-22 1992-07-22 Commande de fonctionnement d'une pompe au moyen de donnees dynagraphiques calculees au fond du puits

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PCT/US1992/006105 Ceased WO1993002289A1 (fr) 1991-07-22 1992-07-22 Commande de fonctionnement d'une pompe au moyen de donnees dynagraphiques calculees au fond du puits

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Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7500390B2 (en) 2005-06-29 2009-03-10 Weatherford/Lamb, Inc. Method for estimating pump efficiency
WO2007041823A1 (fr) 2005-10-13 2007-04-19 Pumpwell Solutions Ltd. Procede et systeme pour l'optimisation de production de fluide de fond de trou
CA2744324C (fr) 2011-06-27 2018-10-16 Pumpwell Solutions Ltd. Systeme et methode pour determiner l'emplacement de la tige polie des pompes alternatives a tige
EA023666B1 (ru) * 2014-04-29 2016-06-30 Институт Систем Управления Национальной Академии Наук Азербайджанской Республики Способ диагностики состояния глубинного насоса
US10145230B2 (en) 2014-10-10 2018-12-04 Henry Research And Development, Llc Systems and methods for real-time monitoring of downhole pump conditions
US10024314B2 (en) 2015-07-30 2018-07-17 General Electric Company Control system and method of controlling a rod pumping unit
US10260500B2 (en) 2017-05-15 2019-04-16 General Electric Company Downhole dynamometer and method of operation
US11619225B2 (en) * 2020-12-08 2023-04-04 International Business Machines Corporation Identifying potential problems in a pumpjack
CN113027387B (zh) * 2021-02-22 2023-04-07 中国石油天然气股份有限公司 一种油井间抽控制系统及方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4800156A (en) * 1985-04-25 1989-01-24 Sankin Kogyo Kabushiki Kaisha Anaerobic germiculture receptacle
US4830970A (en) * 1981-05-16 1989-05-16 Dr. Madaus & Co. Nutrient substrate carrier
US4885253A (en) * 1989-03-27 1989-12-05 Steris Corporation Universal biological indicator system

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3457781A (en) * 1966-08-29 1969-07-29 Phillips Petroleum Co Modified dynamometer
US3343409A (en) * 1966-10-21 1967-09-26 Shell Oil Co Method of determining sucker rod pump performance
US3527094A (en) * 1967-08-04 1970-09-08 Shell Oil Co Apparatus for analyzing sucker-rod wave motion
US3817094A (en) * 1970-07-27 1974-06-18 Mobil Oil Corp Well monitoring apparatus
US3824851A (en) * 1972-06-01 1974-07-23 Mobil Oil Corp Automatic data retrieval system for pumping wells
US3951209A (en) * 1975-06-09 1976-04-20 Shell Oil Company Method for determining the pump-off of a well
US4034807A (en) * 1975-08-12 1977-07-12 Edgar N. Prince Inside pipe wiper
US4015469A (en) * 1976-07-02 1977-04-05 Shell Oil Company Pump-off monitor for rod pump wells
US4034808A (en) * 1976-09-20 1977-07-12 Shell Oil Company Method for pump-off detection
US4286925A (en) * 1979-10-31 1981-09-01 Delta-X Corporation Control circuit for shutting off the electrical power to a liquid well pump
US4363605A (en) * 1980-11-03 1982-12-14 Mills Manuel D Apparatus for generating an electrical signal which is proportional to the tension in a bridle
US4487061A (en) * 1982-12-17 1984-12-11 Fmc Corporation Method and apparatus for detecting well pump-off
US4483188A (en) * 1983-04-18 1984-11-20 Fmc Corporation Method and apparatus for recording and playback of dynagraphs for sucker-rod wells
US4551730A (en) * 1983-04-18 1985-11-05 Fmc Corporation Method and apparatus for entering control points relative to a dynagraph of a well pumping unit
US4507055A (en) * 1983-07-18 1985-03-26 Gulf Oil Corporation System for automatically controlling intermittent pumping of a well
US4583915A (en) * 1983-08-01 1986-04-22 End Devices, Inc. Pump-off controller
US4561299A (en) * 1984-02-13 1985-12-31 Fmc Corporation Apparatus for detecting changes in inclination or acceleration
US4873635A (en) * 1986-11-20 1989-10-10 Mills Manual D Pump-off control

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4830970A (en) * 1981-05-16 1989-05-16 Dr. Madaus & Co. Nutrient substrate carrier
US4800156A (en) * 1985-04-25 1989-01-24 Sankin Kogyo Kabushiki Kaisha Anaerobic germiculture receptacle
US4885253A (en) * 1989-03-27 1989-12-05 Steris Corporation Universal biological indicator system

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