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WO1992022669A1 - Melange de reactifs pour dosage de glucose - Google Patents

Melange de reactifs pour dosage de glucose Download PDF

Info

Publication number
WO1992022669A1
WO1992022669A1 PCT/GB1992/001116 GB9201116W WO9222669A1 WO 1992022669 A1 WO1992022669 A1 WO 1992022669A1 GB 9201116 W GB9201116 W GB 9201116W WO 9222669 A1 WO9222669 A1 WO 9222669A1
Authority
WO
WIPO (PCT)
Prior art keywords
glucose oxidase
reagent mixture
ius
test reagent
mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB1992/001116
Other languages
English (en)
Inventor
Stephen Peter Gullick
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Arkray Factory Ltd
Original Assignee
Hypoguard UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hypoguard UK Ltd filed Critical Hypoguard UK Ltd
Priority to EP92913166A priority Critical patent/EP0591322A1/fr
Priority to JP5500799A priority patent/JPH06510426A/ja
Priority to AU20211/92A priority patent/AU661492B2/en
Publication of WO1992022669A1 publication Critical patent/WO1992022669A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose

Definitions

  • the glucose oxidase is present in from 400 to 550 IUs per 27.5 to 32.5 IUs of peroxidase and the chromogen is o-tolidine which is present in an amount of from 12 to 15 gs per 500,000 IUs of the glucose oxidase.
  • the reagent mixture be put up in a gel matrix, notably a gelatin matrix, which provides from 200 to 400 gs of the matrix on a dry weight basis per 500,000 IUs of the glucose oxidase.
  • a gel matrix notably a gelatin matrix, which provides from 200 to 400 gs of the matrix on a dry weight basis per 500,000 IUs of the glucose oxidase.
  • the reagent mixture may contain other materials as is customary, for example phosphate buffering agents, preservatives, anti-coagulants or surface active agents. Such other materials are typically inert to the material to be tested, the other constituents of the reagent mixture and the products of the reactions and interactions which occur during the test procedure. Such other constituents can be present in the amounts normally used in such reagent mixtures.
  • the chromogenic material will typically be present in an amount of from 12 to 17, notably from 13 to 16, grams per 500,000 IUs of the glucose oxidase. The optimum proportions within these ranges can be determined for any given case and a given carrier by simple trial and error tests.
  • the reagent mixture is preferably put up in a matrix carrier medium so that the material to be assessed can penetrate to the enzyme reagent and the chromogenic material.
  • the matrix can be provided by a natural gum, jelly or gel, for example a gelatin, agar agar, aspic or silica gel; or can be provided by a synthetic polymer gel, for example a cellulosic gel or a polyvinylic resin gel.
  • a gelatin gel as the carrier matrix.
  • the matrix may contain the enzymatic reagent and chromogenic material non-uniformly distributed therein, as when a gel layer is formed which has a high concentration of the gel matrix in its upper layers to act a protective layer or coating for the reagent rich lower layers; or where the enzymatic reagent is located in a separate zone of the matrix from that containing the chromogenic material.
  • the product from the interaction of the material being tested with one or more of the enzymatic reagents diffuses from the enzyme zone into the zone containing the chromogenic material to develop a colour as a separate stage in that zone.
  • the matrix carrying the reagent mixture can be put up in a number of physical forms, for example as test strips or discs in which a pad of the matrix is applied to one face of the strip or disc and the colour resulting from the interaction of the material under assessment and the reagents and materials in the matrix is observed visually against the white background of the support strip or disc or against a separate reference background.
  • the matrix can be put up in a series of zones through which the reagent mixture is distributed so that the interaction of the material to be assessed with the enzyme occurs in one zone and the product of that interaction diffuses to a second zone in which the colour reaction takes place.
  • the reagent mixture matrix can be absorbed or impregnated into the pores of . a porous carrier to one face of which the material to be assessed is applied and the colour developing within the matrix is viewed from the opposite surface of the carrier.
  • the gel matrix is a high molecular weight gelatin which is present in about 4% by dry weight.
  • a low molecular weight gelatin typically with a molecular weight in the range 20,000 to 50,000.
  • the gel content of the reagent mixture composition exceeds about 20% by dry weight, the gel inhibits the diffusion of reaction products through the membrane and hence development cf a colour reflecting the true extent of the interactions which have occurred.
  • the resultant solution was impregnated into a polysulfone resin sheet (0.2 to 0.4 mms thick and having an average pore diameter of 0.2 micrometres and an air permeability of 3 litres per minute per square centimetre at an applied pressure of 10 psig) to provide 5 IUs of glucose oxidase, 3 IUs of peroxidase, 0.2 milligrams of o-tolidine and 4 milligrams of gelatin per square centimetre of the membrane.
  • the same membrane was impregnated with a conventional reagent mixture to provide the conventional level of enzyme and chromogen per square centimetre.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Emergency Medicine (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Paper (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention se rapporte à une composition test de mélange de réactifs comprenant la glucose oxydase et la peroxydase et un colorant chromogène qui interagit avec le peroxyde d'hydrogène à partir de l'oxydation du glucose du sang par la glucose oxydase, caractérisé en ce que la glucose oxydase et la peroxydase sont présentes dans des proportions de 300 à 700 Unités Internationales (UIs) de glucose oxydase et d'au moins 20 Unités Internationales de peroxydase, et en ce que le colorant chromogène est présent dans une quantité de 12 à 20 grammes de colorant chromogène actif pour 500 000 Unités Internationales de glucose oxydase. De préférence, la composition est chargée dans une matrice de gélatine de poids moléculaire faible et est imprégnée dans une membrane support micro-poreuse.
PCT/GB1992/001116 1991-06-19 1992-06-19 Melange de reactifs pour dosage de glucose Ceased WO1992022669A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP92913166A EP0591322A1 (fr) 1991-06-19 1992-06-19 Melange de reactifs pour dosage de glucose
JP5500799A JPH06510426A (ja) 1991-06-19 1992-06-19 グルコース測定用試薬
AU20211/92A AU661492B2 (en) 1991-06-19 1992-06-19 Reagent mixtures for glucose assay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9113212.6 1991-06-19
GB919113212A GB9113212D0 (en) 1991-06-19 1991-06-19 Reagent mixture

Publications (1)

Publication Number Publication Date
WO1992022669A1 true WO1992022669A1 (fr) 1992-12-23

Family

ID=10696932

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1992/001116 Ceased WO1992022669A1 (fr) 1991-06-19 1992-06-19 Melange de reactifs pour dosage de glucose

Country Status (7)

Country Link
EP (1) EP0591322A1 (fr)
JP (1) JPH06510426A (fr)
AU (1) AU661492B2 (fr)
CA (1) CA2110908A1 (fr)
GB (1) GB9113212D0 (fr)
HU (1) HUT65982A (fr)
WO (1) WO1992022669A1 (fr)

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995031730A1 (fr) * 1994-05-18 1995-11-23 Microquest Diagnostics, Inc. Procede de detection rapide d'un analyte
US7338639B2 (en) 1997-12-22 2008-03-04 Roche Diagnostics Operations, Inc. System and method for analyte measurement
US7390667B2 (en) 1997-12-22 2008-06-24 Roche Diagnostics Operations, Inc. System and method for analyte measurement using AC phase angle measurements
US7407811B2 (en) 1997-12-22 2008-08-05 Roche Diagnostics Operations, Inc. System and method for analyte measurement using AC excitation
US7452457B2 (en) 2003-06-20 2008-11-18 Roche Diagnostics Operations, Inc. System and method for analyte measurement using dose sufficiency electrodes
US7488601B2 (en) 2003-06-20 2009-02-10 Roche Diagnostic Operations, Inc. System and method for determining an abused sensor during analyte measurement
US7556723B2 (en) 2004-06-18 2009-07-07 Roche Diagnostics Operations, Inc. Electrode design for biosensor
US7569126B2 (en) 2004-06-18 2009-08-04 Roche Diagnostics Operations, Inc. System and method for quality assurance of a biosensor test strip
US7597793B2 (en) 2003-06-20 2009-10-06 Roche Operations Ltd. System and method for analyte measurement employing maximum dosing time delay
US7604721B2 (en) 2003-06-20 2009-10-20 Roche Diagnostics Operations, Inc. System and method for coding information on a biosensor test strip
WO2012109473A3 (fr) * 2011-02-09 2012-12-13 University Of Massachusetts Détection d'endotoxines
US8877035B2 (en) 2005-07-20 2014-11-04 Bayer Healthcare Llc Gated amperometry methods
US9110013B2 (en) 2005-09-30 2015-08-18 Bayer Healthcare Llc Gated voltammetry methods
US9410917B2 (en) 2004-02-06 2016-08-09 Ascensia Diabetes Care Holdings Ag Method of using a biosensor
US9933385B2 (en) 2007-12-10 2018-04-03 Ascensia Diabetes Care Holdings Ag Method of using an electrochemical test sensor

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000125994A (ja) * 1998-10-28 2000-05-09 Aisin Seiki Co Ltd 樹脂性クッションエレメント

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB893318A (en) * 1959-11-02 1962-04-04 Miles Lab Stable blood detecting composition
FR2295425A1 (fr) * 1974-12-21 1976-07-16 Boehringer Mannheim Gmbh Application de derives de 3,3, 5,5-tetra-alcoylbenzidine en tant qu'indicateurs d'oxydation dans des essais rapides
EP0016947A1 (fr) * 1979-04-04 1980-10-15 Roche Diagnostics GmbH Procédé et réactif pour la détermination enzymatique de substrats enzymatiques
US4340669A (en) * 1981-02-12 1982-07-20 Miles Laboratories, Inc. System for the determination of glucose in fluids

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB893318A (en) * 1959-11-02 1962-04-04 Miles Lab Stable blood detecting composition
FR2295425A1 (fr) * 1974-12-21 1976-07-16 Boehringer Mannheim Gmbh Application de derives de 3,3, 5,5-tetra-alcoylbenzidine en tant qu'indicateurs d'oxydation dans des essais rapides
EP0016947A1 (fr) * 1979-04-04 1980-10-15 Roche Diagnostics GmbH Procédé et réactif pour la détermination enzymatique de substrats enzymatiques
US4340669A (en) * 1981-02-12 1982-07-20 Miles Laboratories, Inc. System for the determination of glucose in fluids

Cited By (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5750357A (en) * 1994-05-18 1998-05-12 Microquest Diagnostics, Inc. Method of rapid analyte detection
WO1995031730A1 (fr) * 1994-05-18 1995-11-23 Microquest Diagnostics, Inc. Procede de detection rapide d'un analyte
US7338639B2 (en) 1997-12-22 2008-03-04 Roche Diagnostics Operations, Inc. System and method for analyte measurement
US7390667B2 (en) 1997-12-22 2008-06-24 Roche Diagnostics Operations, Inc. System and method for analyte measurement using AC phase angle measurements
US7407811B2 (en) 1997-12-22 2008-08-05 Roche Diagnostics Operations, Inc. System and method for analyte measurement using AC excitation
US7494816B2 (en) 1997-12-22 2009-02-24 Roche Diagnostic Operations, Inc. System and method for determining a temperature during analyte measurement
US7604721B2 (en) 2003-06-20 2009-10-20 Roche Diagnostics Operations, Inc. System and method for coding information on a biosensor test strip
US7452457B2 (en) 2003-06-20 2008-11-18 Roche Diagnostics Operations, Inc. System and method for analyte measurement using dose sufficiency electrodes
US7488601B2 (en) 2003-06-20 2009-02-10 Roche Diagnostic Operations, Inc. System and method for determining an abused sensor during analyte measurement
US8859293B2 (en) 2003-06-20 2014-10-14 Roche Diagnostics Operations, Inc. Method for determining whether a disposable, dry regent, electrochemical test strip is unsuitable for use
US7597793B2 (en) 2003-06-20 2009-10-06 Roche Operations Ltd. System and method for analyte measurement employing maximum dosing time delay
US10067082B2 (en) 2004-02-06 2018-09-04 Ascensia Diabetes Care Holdings Ag Biosensor for determining an analyte concentration
US9410917B2 (en) 2004-02-06 2016-08-09 Ascensia Diabetes Care Holdings Ag Method of using a biosensor
US7569126B2 (en) 2004-06-18 2009-08-04 Roche Diagnostics Operations, Inc. System and method for quality assurance of a biosensor test strip
US7556723B2 (en) 2004-06-18 2009-07-07 Roche Diagnostics Operations, Inc. Electrode design for biosensor
US9410915B2 (en) 2004-06-18 2016-08-09 Roche Operations Ltd. System and method for quality assurance of a biosensor test strip
US8877035B2 (en) 2005-07-20 2014-11-04 Bayer Healthcare Llc Gated amperometry methods
US9835582B2 (en) 2005-09-30 2017-12-05 Ascensia Diabetes Care Holdings Ag Devices using gated voltammetry methods
US9110013B2 (en) 2005-09-30 2015-08-18 Bayer Healthcare Llc Gated voltammetry methods
US10670553B2 (en) 2005-09-30 2020-06-02 Ascensia Diabetes Care Holdings Ag Devices using gated voltammetry methods
US11435312B2 (en) 2005-09-30 2022-09-06 Ascensia Diabetes Care Holdings Ag Devices using gated voltammetry methods
US9933385B2 (en) 2007-12-10 2018-04-03 Ascensia Diabetes Care Holdings Ag Method of using an electrochemical test sensor
US10690614B2 (en) 2007-12-10 2020-06-23 Ascensia Diabetes Care Holdings Ag Method of using an electrochemical test sensor
WO2012109473A3 (fr) * 2011-02-09 2012-12-13 University Of Massachusetts Détection d'endotoxines

Also Published As

Publication number Publication date
GB9113212D0 (en) 1991-08-07
HUT65982A (en) 1994-08-29
AU2021192A (en) 1993-01-12
CA2110908A1 (fr) 1992-12-23
HU9303638D0 (en) 1994-04-28
JPH06510426A (ja) 1994-11-24
AU661492B2 (en) 1995-07-27
EP0591322A1 (fr) 1994-04-13

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