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WO1992019719A1 - Bacteries degradant le glyphosate - Google Patents

Bacteries degradant le glyphosate Download PDF

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Publication number
WO1992019719A1
WO1992019719A1 PCT/GB1992/000773 GB9200773W WO9219719A1 WO 1992019719 A1 WO1992019719 A1 WO 1992019719A1 GB 9200773 W GB9200773 W GB 9200773W WO 9219719 A1 WO9219719 A1 WO 9219719A1
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WO
WIPO (PCT)
Prior art keywords
glyphosate
degrading
protein
strain
proteins
Prior art date
Application number
PCT/GB1992/000773
Other languages
English (en)
Inventor
Rosemary Elaine Dick
John Quinn
Sarah Bronwen Rees
Wolfgang Walter Schuch
Original Assignee
Zeneca Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB919109690A external-priority patent/GB9109690D0/en
Priority claimed from GB919113910A external-priority patent/GB9113910D0/en
Application filed by Zeneca Limited filed Critical Zeneca Limited
Publication of WO1992019719A1 publication Critical patent/WO1992019719A1/fr

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    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
    • A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/12—Activated sludge processes
    • C02F3/1205—Particular type of activated sludge processes
    • C02F3/1231—Treatments of toxic sewage
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
    • A62D2101/04—Pesticides, e.g. insecticides, herbicides, fungicides or nematocides
    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
    • A62D2101/20—Organic substances
    • A62D2101/26—Organic substances containing nitrogen or phosphorus
    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
    • A62D2101/20—Organic substances
    • A62D2101/28—Organic substances containing oxygen, sulfur, selenium or tellurium, i.e. chalcogen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/05—Alcaligenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38—Pseudomonas
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Definitions

  • This invention relates to glyphosate-degrading bacteria and their use. More specifically, the invention relates to a novel strain of Alcaligenes xylosoxidans subspecies denitrificans and a novel strain of a Pseudomonas species.
  • Glyphosate (N-phosphonomethylglycine) is the active component of a herbicide sold under the trade name 'Round-up' by the Monsanto Corporation. Glyphosate acts on a highly specific target site: it is a potent inhibitor of 5-enolpyruvylshikimate 3-phosphate synthase (EPSP synthase; EC 2.5.1.19). It is readily translocated within plants and so has good systemic action, it shows limited persistence in the soil, and it has an excellent toxicological profile. In addition, it has proved difficult to synthesise chemical analogues which have the same specificity and efficacy as glyphosate. These factors have contributed greatly to the success and value of glyphosate as a broad spectrum herbicide.
  • EPP synthase 5-enolpyruvylshikimate 3-phosphate synthase
  • Glyphosate has been shown to have no drastic effect on the populations of soil micro-organisms when used at the recommended rates. Although there are many potential pathways for the metabolism of glyphosate, only two pathways have been demonstrated unambiguously. These are (1) the sarcosine pathway, involving the cleavage of the C-P bond and (2) the AMPA pathway involving the cleavage of the C-N bond of the glycyl moiety of glyphosate (Malik et al, 1989, BioFactors, 2, 17-25).
  • SUBSTITUTE SHEET pathway for glyphosate metabolism include Pseudomonas sp. PG2982 (Moore et al, 1983, Appl Environ Microbiol, 46, 316-320). Subsequent studies have demonstrated that this organism can use a wide range of phosphonates as growth substrates (Shinabarger et al, 1984, Appl Environ Microbiol, 48, 1049-1050; Kishore and Jacob, 1987, J Biol Chem, 262, 12164-12168).
  • Glyphosate degradation in Pseudomonas PG2982 is apparently analogous to the bacterial metabolism of alkyl and arylphosphonates involving the C-P lyase enzyme to yield first sarcosine and then glycine with the phosphonomethyl carbon released to Cl metabolism (Malik et al, 1989, BioFactors, 2, 17-25).
  • Glyphosate is metabolised to AMPA in Arthrobacter atrocyaneus (Pipke and Amrhein, 1988,
  • EPSP synthase enzyme has been isolated from selected bacterial strains, such as Salmonella typhimurium.
  • the mutant form of EPSP synthase showed decreased affinity for glyphosate but its kinetic properties were maintained.
  • the gene for the bacterial enzyme was transferred to tobacco and expressed within the plant. These transgenic plants were tolerant to increased levels of the herbicide both in greenhouse and field experiments.
  • EPSP synthase has also been isolated from plants which are naturally resistant to increased levels of glyphosate. It was found that this enzyme was normal, but its over expression led to glyphosate tolerance. Thus transgenic plants produced to give high levels of EPSP synthase expression should show increased glyphosate tolerance.
  • resistance is achieved by manipulating the target site of the herbicide, either by alteration of the target enzyme or by its over-production to reduce the overall effect of inhibition.
  • Herbicide resistance can also be achieved through detoxification by degrading or modifying the herbicide before it acts on its target site. This latter mechanism has been used to generate plants resistant to two other herbicides: bromoxynil and phosphinothricin (Basta).
  • Basta phosphinothricin
  • the identification and isolation of genes coding for proteins which can be used to detoxify the herbicide have played a critical part in these experiments. This was achieved through the isolation of bacterial strains containing enzymes capable of detoxifying the herbicide: Klebsiella sp.
  • An object of the present invention is to provide a source of genes which encode enzymes capable of degrading glyphosate. These genes may be inserted into plants to render them tolerant to the herbicide. A further object is to provide a useful agent for removing unwanted glyphosate from the environment, such as production or spill sites.
  • a glyphosate-degrading agent comprising a novel microorganism which can metabolise glyphosate via the AMPA pathway.
  • a novel glyphosate-degrading strain, SC9, of Alcaligenes xylosoxidans subspecies denitrificans and a novel glyphosate-degrading strain, SCll, of a Pseudomonas species are provided.
  • the invention comprises glyphosate-degrading proteins capable of being isolated from strains SC9 and SCll.
  • the invention also comprises DNA sequences coding for proteins according to the invention.
  • the DNA may be cloned or transformed into a biological system allowing expression of the encoded protein.
  • the invention also comprises plants transformed with recombinant DNA encoding a glyphosate-degrading protein according to the invention.
  • the invention also comprises a process of degrading glyphosate whereby the herbicide is exposed to the strains or proteins according to the invention.
  • the bacterial strains of this invention were isolated from soil.
  • the strains can degrade glyphosate in the course of aerobic growth on minimal medium.
  • NCIMB Bacteria
  • SC9 is a strain of Alcaligenes xylosoxidans subspecies denitrificans.
  • SCll is a strain of a Pseudomonas species but are unable to match it exactly with any of the species described in Bergey's Manual.
  • the bacterial strains may be used to degrade glyphosate in the environment.
  • strains may be further characterised by biochemical methods to indicate the enzyme system responsible for the metabolism of glyphosate.
  • the glyphosate-degrading proteins may be isolated: from their primary structure, the sequence or partial sequence of the DNA encoding them may be determined. This DNA may be manufactured using a standard nucleic acid synthesiser, or suitable probes (derived from the known sequence) may be used to isolate the actual gene(s) and control sequences from the bacterial
  • SUBSTITUTESHEET genome This genetic material may then be cloned into a biological system which allows expression of the proteins.
  • the DNA may be inserted into a suitable micro-organism.
  • the DNA may also be transformed by known methods into any plant species, so that the glyphosate-degrading proteins are expressed within the plant. Suitable plant species include tobacco, maize and sugarbeet. Transformed plants should show increased tolerance to glyphosate.
  • Figure 1 shows a soil percolation column for establishment of glyphosate-degrading cultures
  • Figure 2 is a series of time-course graphs showing glyphosate degradation by strains SC9 and SCll.
  • NCIMB identified SC9 as a strain of Alcaligenes xylosoxidans subspecies denitrificans.
  • NCIMB identified SCll as a strain of a
  • strain SCll may be a Pseudomonas chlororaphis, but the following reactions were atypical of this species: gelatin hydrolysis; levan production; lecithinase; Tween 80 hydrolysis; denitrification; arginine dihydrolase; gluconate utilisation. Also, production of chlororaphin was not observed in either glycerol or King's A medium. In addition, the isolate does not appear to belong to the fluorescent Pseudomonas group (P_ cepacia, P_ stutzeri, P acidovorans or P alcaligenes, J? pseudoalcaligenes) .
  • the columns were incubated at ambient temperature, in darkness to prevent algal growth, for a total of 280 days.
  • the volume was readjusted weekly to 20ml by the addition of sterile distilled water. Further aliquots of sterile glyphosate solution were added as appropriate to achieve a gradual increase in concentration. At intervals, 0.5ml aliquots were removed and examined for change in pH, chemical and microbial composition.
  • Both sets of cultures were incubated at 29°C for 12 days. At intervals, after the addition of sterile distilled water to replace loss through evaporation, samples were taken for analysis.
  • Non-radioactive cultures were examined for microbial numbers and diversity using half-strength soil extract agar (see Experiment 5) and lOmM glyphosate by the method of Harris and
  • Sub-cultured populations from both the treated and untreated columns were plated out on to equivalent solid medium from which micro-organisms SC9 and SCll were isolated from the untreated and treated soil columns respectively.
  • SC9 and SCll were grown on soil extract agar (see Experiment 5) for 5 days after which a lO l loop of culture was removed from each plate and resuspended in soil extract broth containing l Ci ml " 3- 14C-glyphosate and "cold” glyphosate to a final concentration of 100 M. lOO l samples were taken at intervals, centrifuged and the supernatants stored at -20°C. Analysis was by tlc/autoradiography and scintillation counting (see Experiment 6).
  • SC9 and SCll cultures were maintained on agar slants of half-strength soil extract medium.
  • SC9 and SCll were subcultured from half-strength nutrient agar and tested for their ability to use glyphosate and/or AMPA as sole source of phosphorus.
  • 50 ⁇ l of bacterial suspension was used to inoculate 5ml of phosphate-free minimal medium, M3-P (see Experiment 8).
  • the cultures were incubated at 29°C for 5 days to starve the cells of any phosphate reserves. This process was repeated by sub-culture to ensure phosphate depletion. 100 1 aliquots were then taken to inoculate 10ml of each of the following media (a-d) and incubated for 7 days at 29°C.
  • M3-P b. M3-P + ImM phosphate c. M3-P + ImM glyphosate as sole phosphate source d. M3-P + ImM AMPA as sole phosphate source
  • the organisms were also tested for their ability to use 5mM methylphosphonate as sole source of phosphorus.
  • the supernatants of the cultures grown in the presence of glyphosate, AMPA and methylphosphonate were tested for phosphate release after 7 days incubation, by which time growth was complete even in the more slow growing cultures.
  • SUBSTITUTESHEET water added was varied according to the expected phosphate content of the sample.
  • the assay was left for one hour to allow colour to develop before reading the absorbance at 600nm.
  • a set of standards was prepared for each assay.
  • Glyphosate and other phosphonates were detected as total phosphorus by a modification of the method of Ames (1966, Methods Enzymol, 8, 115-118).
  • 100/t/l of a solution of 10% Mg(N0 3 ) 2 in 95% ethanol in a phosphorus-free pyrex boiling tube was heated over a strong bunsen flame to dryness and the organic matter ashed until the evolution of brown fumes had ceased.
  • 1.0ml of 0.5N HCl was added to the residue and the tube was then covered with parafilm and placed in a boiling waterbath for 15 minutes. The resulting solution was tested for phosphorus as previously described.
  • Soil extract medium was prepared by the method of James (1958, Can J Microbiol, 4, 336), using fresh Ballyronan garden soil. It was filter-sterilised before use to ensure removal of heat-resistant spores.
  • Glyphosate was added to a final concentration of lOmM.
  • Half strength soil extract was also used and supplemented with 0.6mg 1 ⁇ glucose plus glyphosate to a final concentration of 10mM.
  • Solvent system (1) was found to give the best separation of glyphosate and its metabolites, but the presence of ammonium resulted in a dark background after development with ninhydrin, reducing sensitivity of visualisation. Consequently, this system was mainly used for the separation of radiolabelled compounds.
  • Solvent system (2) was used for the separation of compounds from non-radioactive cultures.
  • Trizma base (Sigma) 6.0g NH 4 C1 5.0g
  • SUBSTITUTESHEET The solution is heat sterilised at 121°C for 15 minutes. On cooling to 55°C the following supplements were added as filter sterilised solutions (per litre): 5.0g potassium gluconate 5.0g sodium pyruvate 1.0ml vitamin solution.
  • glucose or glycerol replaced gluconate and pyruvate as carbon sources, they were autoclaved separately as concentrated solutions.
  • Filter-sterilised solutions of phosphate, glyphosate or other phosphonates are added as phosphorus sources.
  • Phosphate is added as freshly-prepared buffered solution, pH 7.0, filter-sterilised and diluted accordingly.
  • Glyphosate and other phosphonates were prepared as concentrated solutions, adjusted to pH 7.0 by the addition of dilute NaOH, and filter-sterilised.

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Abstract

Nouvelles souches bactériennes, SC9 (Alcaligenes xylosoxidans, sous-espèces denitrificans) et SC11 (souche d'une espèce peu commune de Pseudomonas) isolées. Ces souches peuvent dégrader le glyphosate herbicide et peuvent servir à produire des protéines dégradant le glyphosate. Les bactéries sont des sources de gènes codant pour des protéines dégradant le glyphosate, qui peuvent être utilisées pour la production de plantes résistant aux herbicides.
PCT/GB1992/000773 1991-05-03 1992-04-27 Bacteries degradant le glyphosate WO1992019719A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB919109690A GB9109690D0 (en) 1991-05-03 1991-05-03 Glyphosphate degrading bacteria
GB9109690.9 1991-05-03
GB9113910.5 1991-06-27
GB919113910A GB9113910D0 (en) 1991-06-27 1991-06-27 Glyphosate degrading bacteria

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WO1992019719A1 true WO1992019719A1 (fr) 1992-11-12

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AU (1) AU1650092A (fr)
WO (1) WO1992019719A1 (fr)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2740347A1 (fr) * 1995-10-27 1997-04-30 France Etat Procede de transformation d'un compose organophosphore toxique en composes denues de toxicite a l'aide de microorganismes
US5928995A (en) * 1995-05-05 1999-07-27 E. I. Du Pont De Nemours And Company Herbicidal mixtures
WO2002063958A1 (fr) * 2001-02-14 2002-08-22 Pedro Brito Correia Procede destine a ameliorer la biodegradabilite dans le sol des produits agrochimiques
EP1927655A1 (fr) * 2006-11-29 2008-06-04 Flughafen München GmbH Procédé de sélection de microorganismes isolés au sol
EP2021359A4 (fr) * 2006-05-12 2009-11-25 Commw Scient Ind Res Org Enzymes capables de dégrader les herbicides
CN104998899A (zh) * 2015-07-07 2015-10-28 东南大学 一种基于草甘膦降解的重金属离子矿化修复的微生物方法
CN112725213A (zh) * 2020-06-22 2021-04-30 辽宁省微生物科学研究院 一种节杆菌及其作为腐熟蔬菜秸秆的腐熟剂的应用
CN113234626A (zh) * 2021-05-13 2021-08-10 安徽省农业科学院水产研究所 一种具有异养硝化-好氧反硝化功能的菌株及其应用
CN113604411A (zh) * 2021-10-08 2021-11-05 山东益生种畜禽股份有限公司 草甘膦降解菌及其应用
US20250136924A1 (en) * 2023-10-26 2025-05-01 Gene Guard Detox, Inc. Food grade bacteria and methods for removing glyphosate and other harmful substances

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992000377A1 (fr) * 1990-06-25 1992-01-09 Monsanto Company Plantes tolerant le glyphosate

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992000377A1 (fr) * 1990-06-25 1992-01-09 Monsanto Company Plantes tolerant le glyphosate

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
APPPLIED AND ENVIRONMENTAL MICROBIOLOGY vol. 54, no. 12, December 1988, pages 2953 - 2958; JACOB, G.S., ET AL.: 'Metabolism of glyphosate in Pseudomonas sp. strain LBr' *
BIOFACTORS vol. 2, no. 1, 1989, pages 17 - 25; MALIK, J., ET AL.: 'The herbicide glyphosate' *
Biological Abstracts, vol.87, 1989, ref.25886 *
CURRENT MICROBIOLOGY vol. 10, 1984, pages 255 - 260; TALBOT, H.W., ET AL.: 'Glyphosate utilization by Pseuudomonas sp. and Alcaligenes sp. isolated from environmental sources' *
J. CELL. BIOCHEM. SUPPL. vol. 13D, 1989, page 338; MCLEAN, P.A., ET AL.: 'Toward herbicide resistant plants: cloning of the genes for glyphosate degradation from a soil organism, and their expression in E.coli' *
TRENDS IN GENETICS vol. 4, no. 8, August 1988, pages 219 - 222; BOTTERMAN, J., ET AL.: 'Engineering herbicide resistance in plants' *

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5928995A (en) * 1995-05-05 1999-07-27 E. I. Du Pont De Nemours And Company Herbicidal mixtures
FR2740347A1 (fr) * 1995-10-27 1997-04-30 France Etat Procede de transformation d'un compose organophosphore toxique en composes denues de toxicite a l'aide de microorganismes
WO2002063958A1 (fr) * 2001-02-14 2002-08-22 Pedro Brito Correia Procede destine a ameliorer la biodegradabilite dans le sol des produits agrochimiques
EP2021359A4 (fr) * 2006-05-12 2009-11-25 Commw Scient Ind Res Org Enzymes capables de dégrader les herbicides
EP1927655A1 (fr) * 2006-11-29 2008-06-04 Flughafen München GmbH Procédé de sélection de microorganismes isolés au sol
CN104998899B (zh) * 2015-07-07 2017-03-15 东南大学 一种采用微生物降解草甘膦用于重金属离子矿化的方法
CN104998899A (zh) * 2015-07-07 2015-10-28 东南大学 一种基于草甘膦降解的重金属离子矿化修复的微生物方法
CN112725213A (zh) * 2020-06-22 2021-04-30 辽宁省微生物科学研究院 一种节杆菌及其作为腐熟蔬菜秸秆的腐熟剂的应用
CN112725213B (zh) * 2020-06-22 2023-08-29 辽宁省微生物科学研究院 一种节杆菌及其作为腐熟蔬菜秸秆的腐熟剂的应用
CN113234626A (zh) * 2021-05-13 2021-08-10 安徽省农业科学院水产研究所 一种具有异养硝化-好氧反硝化功能的菌株及其应用
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US20250136924A1 (en) * 2023-10-26 2025-05-01 Gene Guard Detox, Inc. Food grade bacteria and methods for removing glyphosate and other harmful substances

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