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WO1992016180A3 - Detection de la resistance a la 3'-azido-3'-desoxythymidine - Google Patents

Detection de la resistance a la 3'-azido-3'-desoxythymidine Download PDF

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Publication number
WO1992016180A3
WO1992016180A3 PCT/US1992/002037 US9202037W WO9216180A3 WO 1992016180 A3 WO1992016180 A3 WO 1992016180A3 US 9202037 W US9202037 W US 9202037W WO 9216180 A3 WO9216180 A3 WO 9216180A3
Authority
WO
WIPO (PCT)
Prior art keywords
resistance
detection
deoxythymidine
azido
assay
Prior art date
Application number
PCT/US1992/002037
Other languages
English (en)
Other versions
WO1992016180A2 (fr
Inventor
Thomas Raymond Gingeras
Kevin J Barringer
Douglas D Richman
Patricia C Prodanovich
Geneva Ruth Davis
Original Assignee
Siska Diagnostics Inc
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Siska Diagnostics Inc, Univ California filed Critical Siska Diagnostics Inc
Publication of WO1992016180A2 publication Critical patent/WO1992016180A2/fr
Publication of WO1992016180A3 publication Critical patent/WO1992016180A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • AIDS & HIV (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Méthode de détection du génotype des allèles mutants et de phénotype sauvage au niveau d'une pluralité d'emplacements associés à la résistance au médicament AZT. La méthode consiste à utiliser une série de sondes aptes à s'hybrider à une région amplifiée du gène pol du VIH-1. La structure de méthode consistant à amplifier un acide nucléique rare puis à le capturer sur une phase solide, ainsi qu'à détecter les séquences capturées à l'aide d'une sonde marquée, s'applique aux méthodes déterminant la résistance à d'autres médicaments et impliquant des mutations ponctuelles.
PCT/US1992/002037 1991-03-13 1992-03-12 Detection de la resistance a la 3'-azido-3'-desoxythymidine WO1992016180A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US66854991A 1991-03-13 1991-03-13
US668,549 1991-03-13
US75414691A 1991-09-03 1991-09-03
US754,146 1991-09-03

Publications (2)

Publication Number Publication Date
WO1992016180A2 WO1992016180A2 (fr) 1992-10-01
WO1992016180A3 true WO1992016180A3 (fr) 1992-10-29

Family

ID=27099938

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1992/002037 WO1992016180A2 (fr) 1991-03-13 1992-03-12 Detection de la resistance a la 3'-azido-3'-desoxythymidine

Country Status (2)

Country Link
AU (1) AU1741692A (fr)
WO (1) WO1992016180A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5733781A (en) * 1994-07-19 1998-03-31 Gen-Probe Incorporated Oligonucleotides and methods for inhibiting propagation of human immunodeficiency virus
US5599662A (en) * 1995-02-17 1997-02-04 Hoffmann-La Roche Inc. Oliconucleotide primers and probes for the detection of HIV-1
US6265152B1 (en) * 1995-12-22 2001-07-24 Visible Genetics Inc. Method and kit for evaluation of HIV mutations
BR9704637A (pt) * 1996-01-26 1998-06-09 Innogenetics Nv Processo para deteção de mutações induzidas por droga no gene de transcrição reversa
US6830887B2 (en) 1997-03-18 2004-12-14 Bayer Healthcare Llc Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
US5962665A (en) * 1997-06-16 1999-10-05 Abbott Laboratories Nucleic acid primers and probes for detecting HIV-1 and HIV-2
JP2001518313A (ja) * 1997-09-26 2001-10-16 ビジブル ジェネティクス インコーポレイテッド Ｈｉｖ突然変異の評価方法およびキット
WO2003018835A2 (fr) * 2001-08-23 2003-03-06 Hvidovre Hospital Procede de detection rapide d'haplotypes
ATE549421T1 (de) * 2003-12-19 2012-03-15 Gen Probe Inc Zusammensetzungen, verfahren und kits zum nachweis der nukleinsäuren von hiv-1 und hiv-2
EP2035578A4 (fr) * 2006-06-09 2010-01-27 Conexio 4 Pty Ltd Identification d'une molécule d'acide nucléique

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0422762A2 (fr) * 1989-08-09 1991-04-17 The Wellcome Foundation Limited Procédé pour la vérification de la sensibilité d'HIV-1 pour zidovudine et d'oligonucléotides pour cela
WO1991010746A1 (fr) * 1990-01-10 1991-07-25 Chiron Corporation Transcriptions d'arn-polymerase dependante d'adn servant de molecules reportees pour l'amplification de signaux dans les analyses d'hybridation d'acide nucleique
WO1991013075A2 (fr) * 1990-02-16 1991-09-05 Orion-Yhtymä Oy Procede et reactif permettant de determiner des variations specifique de nucleotides

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0422762A2 (fr) * 1989-08-09 1991-04-17 The Wellcome Foundation Limited Procédé pour la vérification de la sensibilité d'HIV-1 pour zidovudine et d'oligonucléotides pour cela
WO1991010746A1 (fr) * 1990-01-10 1991-07-25 Chiron Corporation Transcriptions d'arn-polymerase dependante d'adn servant de molecules reportees pour l'amplification de signaux dans les analyses d'hybridation d'acide nucleique
WO1991013075A2 (fr) * 1990-02-16 1991-09-05 Orion-Yhtymä Oy Procede et reactif permettant de determiner des variations specifique de nucleotides

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
AIDS vol. 5, no. 2, February 1991, pages 137 - 144; B.A.LARDER ET AL.: 'Zidovudine resistance by direct detection of mutations in DNA from HIV-infected lymphocytes' *
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA. vol. 87, March 1990, WASHINGTON US pages 1874 - 1878; J.C.GUATELLI ET AL.: 'Isothermal,in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication' cited in the application *
SCIENCE. vol. 246, 1 December 1989, LANCASTER, PA US pages 1155 - 1158; B.A.LARDER ET AL.: 'Multiple mutations in HIV-1 reverse transcriptase confer high-level resistance to zidovudine (AZT)' *
THE JOURNAL OF INFECTIOUS DISEASES vol. 164, no. 6, December 1991, CHICAGO,USA pages 1066 - 1074; T.R.GINGERAS ET AL.: 'Use of self-sustained sequence replication amplification reaction to analyze and detect mutations in zidovudine-resistant human immunodeficiency virus' *
THE JOURNAL OF INFECTIOUS DISEASES vol. 164, no. 6, December 1991, CHICAGO,USA pages 1075 - 1081; D.R.RICHMANN ET AL.: 'Detection of mutations associated with Zidovudine resistance in human immunodeficiency virus by use of the polymerase chain reaction' *
WORLD PATENTS INDEX LATEST Week 1491, 22 May 1991 Derwent Publications Ltd., London, GB; AN 91-094596 & AU,A,9 060 828 (WELLCOME FOUNDATION LTD) 14 February 1991 *

Also Published As

Publication number Publication date
WO1992016180A2 (fr) 1992-10-01
AU1741692A (en) 1992-10-21

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