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WO1992002815A3 - Quantitative analysis and monitoring of protein structure by subtractive chromatography - Google Patents

Quantitative analysis and monitoring of protein structure by subtractive chromatography Download PDF

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Publication number
WO1992002815A3
WO1992002815A3 PCT/US1991/005544 US9105544W WO9202815A3 WO 1992002815 A3 WO1992002815 A3 WO 1992002815A3 US 9105544 W US9105544 W US 9105544W WO 9202815 A3 WO9202815 A3 WO 9202815A3
Authority
WO
WIPO (PCT)
Prior art keywords
concentration
monitoring
sample
quantitative analysis
solution
Prior art date
Application number
PCT/US1991/005544
Other languages
French (fr)
Other versions
WO1992002815A2 (en
Inventor
Noubar B Afeyan
Fred E Regnier
Original Assignee
Perseptive Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perseptive Biosystems Inc filed Critical Perseptive Biosystems Inc
Priority to CA002088360A priority Critical patent/CA2088360A1/en
Priority to AU85306/91A priority patent/AU654503B2/en
Publication of WO1992002815A2 publication Critical patent/WO1992002815A2/en
Publication of WO1992002815A3 publication Critical patent/WO1992002815A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N2030/626—Detectors specially adapted therefor calibration, baseline
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Methods are provided for making real-time determinations of the concentration and for monitoring the structural profile of a target solute in a solution. Non-diffusion bound affinity chromatographic techniques are used to generate chromatograms having sharply defined breakthrough curves. Based on the difference between the sensed equilibrium concentration of the impurities which break through the matrix and the concentration of all solutes in the sample, the concentration of the target solute in a solution can be determined. Alternatively, a chromatogram representative of the structural profile of a protein in a sample comprising a mixture of structural variants may be produced using the method of the invention. The assays may be carried out in real-time so that production or purification of a valuable substance such as a pharmaceutical recombinant protein or the like can be monitored meaningfully.
PCT/US1991/005544 1990-08-10 1991-08-01 Quantitative analysis and monitoring of protein structure by subtractive chromatography WO1992002815A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA002088360A CA2088360A1 (en) 1990-08-10 1991-08-01 Quantitative analysis and monitoring of protein structure by substractive chromatography
AU85306/91A AU654503B2 (en) 1990-08-10 1991-08-01 Quantitative analysis and monitoring of protein structure by subtractive chromatography

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US56612190A 1990-08-10 1990-08-10
US566,121 1990-08-10

Publications (2)

Publication Number Publication Date
WO1992002815A2 WO1992002815A2 (en) 1992-02-20
WO1992002815A3 true WO1992002815A3 (en) 1992-04-30

Family

ID=24261583

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1991/005544 WO1992002815A2 (en) 1990-08-10 1991-08-01 Quantitative analysis and monitoring of protein structure by subtractive chromatography

Country Status (5)

Country Link
EP (1) EP0548178A1 (en)
JP (1) JPH06500396A (en)
AU (1) AU654503B2 (en)
CA (1) CA2088360A1 (en)
WO (1) WO1992002815A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2083820A1 (en) * 1991-03-28 1992-09-29 Noubar B. Afeyan On-line product identification in a chromatography effluent by substraction
US6054047A (en) 1998-03-27 2000-04-25 Synsorb Biotech, Inc. Apparatus for screening compound libraries
CA2240325A1 (en) 1998-03-27 1998-11-14 Synsorb Biotech, Inc. Methods for screening compound libraries
US6720190B1 (en) 1998-03-27 2004-04-13 Ole Hindsgaul Methods for screening compound libraries
US6613575B1 (en) 1998-03-27 2003-09-02 Ole Hindsgaul Methods for screening compound libraries
DE19945351C2 (en) 1999-09-22 2002-04-18 Lohmann Therapie Syst Lts Process for finding and isolating pharmacologically active compounds from mixtures of substances
EP3702775A1 (en) * 2009-06-26 2020-09-02 Cytiva Sweden AB Method for determining binding capacities in a chromatography system
SG11202103572VA (en) * 2018-10-09 2021-05-28 C Technologies Inc Mass control system for chromatography
CN114280208B (en) * 2021-11-03 2024-05-24 鼎康(武汉)生物医药有限公司 Method for measuring dynamic load of affinity chromatography packing

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4204424A (en) * 1979-01-08 1980-05-27 Phillips Petroleum Company Chromatographic analyzer detector gain adjustment
EP0141259A1 (en) * 1981-03-09 1985-05-15 Environmental Sciences Associates, Inc. Improvements in liquid chromatography
WO1990006516A1 (en) * 1988-12-05 1990-06-14 Primus Corporation Method for determination of glycated proteinaceous species

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56114761A (en) * 1980-02-14 1981-09-09 Shinotesuto Kenkyusho:Kk Fractional determination method of zinc in fresh blood serum by affinity chromatography
JPS58216954A (en) * 1982-06-11 1983-12-16 Shimadzu Corp Low temperature high performance liquid chromatography
JPS5984828A (en) * 1982-11-08 1984-05-16 Suntory Ltd Stabilized immune complex and its use
IT1150598B (en) * 1982-12-14 1986-12-17 Ines Bianchi PROCEDURE FOR THE PURIFICATION OF SUPEROXIDE DYSMUTASE BY AFFINITY CHROMATOGRAPHY
JPS59143958A (en) * 1983-02-07 1984-08-17 Sekisui Chem Co Ltd Quantitative analysis of fibronectin
JPS63236964A (en) * 1987-03-26 1988-10-03 Ube Ind Ltd How to diagnose diabetes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4204424A (en) * 1979-01-08 1980-05-27 Phillips Petroleum Company Chromatographic analyzer detector gain adjustment
EP0141259A1 (en) * 1981-03-09 1985-05-15 Environmental Sciences Associates, Inc. Improvements in liquid chromatography
WO1990006516A1 (en) * 1988-12-05 1990-06-14 Primus Corporation Method for determination of glycated proteinaceous species

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BIOTECHNOLOGY vol. 8, no. 3, March 1990, NEW YORK US pages 203 - 206; AFEYAN ET AL.: 'perfusion chromatography an approach to purifying biomolecules' *
JOURNAL OF BIOLOGICAL CHEMISTRY. vol. 259, no. 6, 1984, BALTIMORE US pages 3796 - 3799; YIN CHANG LIU ET AL.: 'quantitative analysis of protein: immobilized dye interaction' *
JOURNAL OF CHROMATOGRAPHY. vol. 512, 20 July 1990, AMSTERDAM NL pages 365 - 376; LLOYD ET AL.: 'preparative high-performance liquid chromatography on a unique high-speed macroporous resign' *

Also Published As

Publication number Publication date
WO1992002815A2 (en) 1992-02-20
EP0548178A1 (en) 1993-06-30
AU654503B2 (en) 1994-11-10
JPH06500396A (en) 1994-01-13
AU8530691A (en) 1992-03-02
CA2088360A1 (en) 1992-02-11

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