[go: up one dir, main page]

WO1992001775A1 - Preparation liquide d'enzymes - Google Patents

Preparation liquide d'enzymes Download PDF

Info

Publication number
WO1992001775A1
WO1992001775A1 PCT/EP1991/001334 EP9101334W WO9201775A1 WO 1992001775 A1 WO1992001775 A1 WO 1992001775A1 EP 9101334 W EP9101334 W EP 9101334W WO 9201775 A1 WO9201775 A1 WO 9201775A1
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme
enzyme preparation
enzymes
weight
preparation according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP1991/001334
Other languages
German (de)
English (en)
Inventor
Wolfgang Drees
Albrecht Weiss
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Henkel AG and Co KGaA
Original Assignee
Henkel AG and Co KGaA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Henkel AG and Co KGaA filed Critical Henkel AG and Co KGaA
Publication of WO1992001775A1 publication Critical patent/WO1992001775A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38663—Stabilised liquid enzyme compositions

Definitions

  • the invention relates to a liquid, essentially anhydrous enzyme preparation which can be used, for example, as a stock solution for the production of liquid detergents or the like.
  • enzyme solutions of hydrolases, especially proteases are not per se stable in storage. This applies in particular to such enzyme Solutions which are to be used for liquid detergents, since in the purification of the enzyme solutions apparently, in addition to impurities, substances which cause stabilization are also removed.
  • German patent application P 39 18 761 proposes a liquid, water-containing enzyme preparation which contains 1.5 to 20% by weight hydrolases, 20 to 40% by weight propylene glycol and 0.5 to 5% by weight boric acid and / or contains their soluble salts.
  • the enzyme concentrate described there is in the form of a solution which also necessarily contains enzyme stabilizers.
  • the invention thus relates to an essentially anhydrous, liquid enzyme preparation containing 5 to 20% by weight of enzymes, 20 to 60% by weight of precipitant and ad 100% by weight of organic water-miscible solvents.
  • the enzyme preparations according to the invention are suitable for the assembly of a large number of enzymes.
  • Liquid preparations of hydrolases can preferably be prepared in this way.
  • the proteases in particular the proteases of the subtilisin type, are preferred among the hydrolases, but other proteases, such as metalloproteases or animal proteases, can also be prepared in this way.
  • proteases such as those in detergents, in particular liquid detergents, are currently preferred be used.
  • related hydrolases such as, for example, ylases, lipases, cellulases or the like, can also be packaged in this way.
  • the enzymes are dissolved in the organic water-miscible solvent and precipitated with a precipitant, so that they are present as a dispersion. It is preferred to carry out the precipitation step with stirring in order to ensure fine particle size.
  • Alcohols are primarily suitable as organic water-dilutable solvents, and among these in particular polyfunctional alcohols.
  • Particularly preferred polyfunctional alcohols are propylene glycol and / or glycerol.
  • other glycols and the di- and trimers of the abovementioned are also suitable.
  • the person skilled in the art can easily determine the solubility by means of a preliminary test. In the vast majority of cases, however, 1,2-propylene glycol has proven to be the most suitable proposal.
  • surfactants have proven themselves primarily as precipitants, since these prevent or delay sedimentation of the enzyme preparations according to the invention which are present in dispersion.
  • nonionic surfactants are preferred.
  • suitable surfactants the person skilled in the art will primarily use products which are liquid at room temperature. Ethoxylates of alcohols with 6 to 20 C atoms or of alkylphenols with 8 to 12 C atoms in the alkyl part have proven successful. It is known that such products can be produced with any degree of ethoxylation.
  • the low-ethoxylated types (1 to 10 mol, in particular 2 to 5 mol, ethylene oxide / mol alcohol or alkylphenol) are preferred for the purposes of the invention.
  • the enzyme preparations according to the invention can also contain further auxiliaries.
  • substances against loss of activity of the enzyme can also be used.
  • boric acid and its salts, insofar as they are soluble or dispersible in the preparations can be added.
  • Lower carboxylic acids, in particular polyfunctional carboxylic acids such as tartaric acid or citric acid, can also be present. The amount of such substances should not exceed 10% by weight of the total preparation and should be at least 1% by weight.
  • the corresponding enzymes in solid form are used, which can be obtained by precipitation or drying of enzyme solutions.
  • the solid enzymes are then first dissolved in the water-miscible organic solvent and the precipitant is added with stirring until a dispersion has formed. If desired, further auxiliaries can then be added. It is possible to heat slightly during the dissolving process and to cool during the precipitation step, but the low temperature stability of the enzymes above about 60 ° C. must be taken into account.
  • the enzyme preparations according to the invention can be used as a stock solution in the production of liquid detergents and the like.
  • a subtilisin protease which is customary in detergents, was precipitated from concentrated solution in a customary manner, filtered off, dispersed in acetone and filtered off again.
  • 1 g of the enzyme powder thus obtained with the specific protease activity of 2,500,000 HPE / g was then dissolved in 5 g of 1,2-propylene glycol with stirring over a period of 2 hours. With further stirring, 6.6 g of an ethoxylated tallow alcohol (3 mol EO / ol tallow alcohol) were then added. Due to the addition of tallow alcohol, the enzyme precipitated out in a very finely divided form without sedimentation. The result was a thin, well pourable dispersion with a specific activity of 193,000 HPE / g.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Detergent Compositions (AREA)

Abstract

Les solutions fondamentales d'enzymes devraient être améliorées quant à leur durée de conservation. A cette fin est proposée une préparation liquide d'enzymes essentiellement anhydre, contenant 5 à 20 % en poids d'enzymes, 20 à 60 % en poids de précipitant et, complétée, pour obtenir 100 % en poids solvants organiques miscibles avec l'eau.
PCT/EP1991/001334 1990-07-25 1991-07-17 Preparation liquide d'enzymes Ceased WO1992001775A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19904023601 DE4023601A1 (de) 1990-07-25 1990-07-25 Fluessige enzymzubereitung
DEP4023601.3 1990-07-25

Publications (1)

Publication Number Publication Date
WO1992001775A1 true WO1992001775A1 (fr) 1992-02-06

Family

ID=6410976

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1991/001334 Ceased WO1992001775A1 (fr) 1990-07-25 1991-07-17 Preparation liquide d'enzymes

Country Status (2)

Country Link
DE (1) DE4023601A1 (fr)
WO (1) WO1992001775A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994025560A1 (fr) * 1993-05-05 1994-11-10 Allied Colloids Limited Dispersions d'enzymes, leur production et compositions les contenant

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11503465A (ja) * 1996-01-31 1999-03-26 ギスト ブロカデス ベスローテン フェンノートシャップ 安定化された生物学的に有効な化合物を含む組成物の使用
CA2388541A1 (fr) * 1999-04-01 2000-10-12 The Procter & Gamble Company Composition de detergent contenant une metallo-protease
WO2013148492A1 (fr) 2012-03-29 2013-10-03 Novozymes A/S Utilisation d'enzymes pour préparer des films hydrosolubles

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3472783A (en) * 1966-02-02 1969-10-14 Winston B Smillie Nonionic detergent compositions
DE1957010A1 (de) * 1969-11-13 1971-06-16 Roehm Gmbh Biologisch aktive,fluessige oder halbfluessige Reinigungsmittel
US3860536A (en) * 1970-01-02 1975-01-14 Cpc International Inc Enzyme-detergent combination
WO1988006183A1 (fr) * 1987-02-13 1988-08-25 Röhm Gmbh Compositions liquides d'enzymes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3472783A (en) * 1966-02-02 1969-10-14 Winston B Smillie Nonionic detergent compositions
DE1957010A1 (de) * 1969-11-13 1971-06-16 Roehm Gmbh Biologisch aktive,fluessige oder halbfluessige Reinigungsmittel
US3860536A (en) * 1970-01-02 1975-01-14 Cpc International Inc Enzyme-detergent combination
WO1988006183A1 (fr) * 1987-02-13 1988-08-25 Röhm Gmbh Compositions liquides d'enzymes

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994025560A1 (fr) * 1993-05-05 1994-11-10 Allied Colloids Limited Dispersions d'enzymes, leur production et compositions les contenant
AU687395B2 (en) * 1993-05-05 1998-02-26 Ciba Specialty Chemicals Water Treatments Limited Enzyme dispersions, their production and compositions containing them

Also Published As

Publication number Publication date
DE4023601A1 (de) 1992-01-30

Similar Documents

Publication Publication Date Title
DE69003253T2 (de) Enzymhaltige zubereitung und eine solche zubereitung enthaltendes detergens.
DE69122789T2 (de) Wässrige Bleichmittelzusammensetzung
DE1808834B2 (de) Stabilisiertes, wäßriges Präparat der Enzyme Protease, a -Amylase oder deren Gemische
DE1964088B2 (de) Stabilisierte wässerige Enzymmischung
CH630406A5 (en) Enzymic liquid washing agent
DE2420009A1 (de) Bestaendige bleichmittelmischung
EP0629692B1 (fr) Préparations enzymatiques liquides
EP1717302B1 (fr) Compositions détergentes liquides avec additif de blanchiment
AT394864B (de) Im wesentlichen wasserfreies, fluessiges grobwaschmittel und verfahren zu seiner herstellung
CH659478A5 (de) Stabilisiertes, klares, einphasiges, builder und enzym enthaltendes waessriges fluessigwaschmittel.
EP0482046B1 (fr) Produit de nettoyage enzymatique
DE3623943A1 (de) Stabilisiertes, textilweichmachendes, enzymhaltiges fluessiges waschmittel
WO1992001775A1 (fr) Preparation liquide d'enzymes
DE4218050A1 (de) Verfahren zur Entwässerung von feinteiligen Feststoffsuspensionen
DE19640201A1 (de) Mittel zur Reinigung von Oberflächen
DE102007039651A1 (de) Bleichkatalysatorgranulat
DE3623942A1 (de) Stabilisiertes, enzymhaltiges fluessiges waschmittel
DE102016204061A1 (de) Reinigungsmittel enthaltend Protease und Amylase
EP0642651B1 (fr) Procede de deshydratation de suspensions de matieres solides finement divisees
CH505891A (de) Verfahren zur Herstellung eines freifliessenden, enzym- und detergenshaltigen Granulats
DE1234908B (de) Keimtoetendes Reinigungspraeparat
EP1067176B1 (fr) Détergent de lavage concentré, liquide, portionable et sans eau
EP0848746B1 (fr) Nettoyant aqueux
DE69007121T2 (de) Enzymhaltige flüssige waschmittelzusammensetzungen und deren verwendung.
DE102019207890A1 (de) Schaumstabilisierung durch spezifisches Tensidgemisch

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): JP US

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): AT BE CH DE DK ES FR GB GR IT LU NL SE