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WO1992000004A1 - Procedes et appareils relatifs a la micropropagation - Google Patents

Procedes et appareils relatifs a la micropropagation Download PDF

Info

Publication number
WO1992000004A1
WO1992000004A1 PCT/GB1991/001043 GB9101043W WO9200004A1 WO 1992000004 A1 WO1992000004 A1 WO 1992000004A1 GB 9101043 W GB9101043 W GB 9101043W WO 9200004 A1 WO9200004 A1 WO 9200004A1
Authority
WO
WIPO (PCT)
Prior art keywords
void
plant
image
base region
cutting line
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB1991/001043
Other languages
English (en)
Inventor
Paul Frank Davis
Robin Deirdre Tillett
John Arthur Marchant
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BTG International Ltd
National Research Development Corp UK
Original Assignee
National Research Development Corp UK
British Technology Group Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Research Development Corp UK, British Technology Group Ltd filed Critical National Research Development Corp UK
Publication of WO1992000004A1 publication Critical patent/WO1992000004A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/003—Cutting apparatus specially adapted for tissue culture

Definitions

  • a sum of the ten specified functions weighted with empirically chosen parameters forms a discriminant function by which a void, with a cutting line extending to it, is selected by choosing the void with the largest discriminant function.
  • the discriminant function is partly comprised of the following three sums, namely:- a weighted sum of functions of the horizontal position of the void which has its greatest value at what may be regarded as a preferred horizontal position; a weighted sum of the functions of the height of the void which is a quadratic function with a maximum value at what may be regarded as a preferred height; and a weighted sum of the functions of the slope of the proposed cutting line which is a quadratic function with a maximum at what may be regarded as a preferred slope.
  • the said minimising step comprises minimising the sum of the squares of the horizontal distances on either side of the line, of the component pixels of the void or the selected part thereof.
  • the method includes formulating for the base region of the plant a line which minimises a measure of the horizontal distances on either side of the line, of the component pixels of the base region of the plant or a selected part thereof, and setting the slope of the cutting line with reference to the said line.
  • the said minimising step comprises minimising the sum of the squares of the horizontal distances on either side of the line, of the component pixels of the base region of the plant or the selected part thereof.
  • apparatus for use in micropropagation, comprising means for generating an image signal representing an image of a plant comprising shoots growing from a base region of the plant; and signal processing means for processing the image signal to locate voids in the image of the plant, to select a void suitable to include the starting point of a cutting line for cutting the base region of the plant, and to generate an output signal containing information as to the location of a cutting line extending from the selected void through the base region of the plant; the signal processing means being arranged to select the said void by reference to: (i) the area of the void or one or more selected parts of the void, alone or in combination with the area of one or more neighbouring voids or selected parts thereof, and/or (ii) the horizontal location of the void; and/or (
  • processing means for processing the image signal to locate a selected void in the image corresponding to a gap between shoots of the plant, to define a cutting line extending from the selected void through the base region, and to generate an output signal containing information as to the location of the said cutting line; the signal processing means being arranged to determine the angle of inclination of the cutting line by reference to:- the shape and/or size of the void; and/or the shape and/or size of the base region of the plant.
  • Figure 3b shows a more complicated image in which the clustering of the shoots is less obvious, but nevertheless the selected cutting line separates two distinct clusters of shoots.
  • the inclination of the cutting line in Figure 3b ensures that a reasonable size piece of callus remains attached to the smaller cluster.
  • r y 3 / (x 2 + y 2 ) 2 .
  • x and y are respectively the horizontal and vertical displacements of the neighbouring void from the original void as measured between the pivot points selected for each void.
  • the preferred discriminant function will now be described further in terms of a formula for a void number j.
  • the formula for the discriminant function for the void includes functions of the size and proximity of
  • the slope estimated for a cutting line is nearly always acceptable, even if not ideal, with the simple arrangements described above.
  • the location of pivot points can be improved in some voids by using more of the context of the location in positioning the pivot points.
  • the pivot point In some of the wider gaps between clusters of stems, the pivot point has been found to be too far from the centre of the gap, and it would be advantageous to allow the region of void used in calculating the pivot point to be deeper in at least some of the wide gaps.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Image Processing (AREA)

Abstract

Un procédé pour tailler une plante comprenant des pousses et un cal consiste à produire un signal d'image représentant une image de la plante par l'intermédiaire d'une caméra vidéo, et à traiter le signal d'image pour situer les vides entre les pousses de la plante et définir une ligne de taille partant du vide choisi et passant par le cal de la plante. On choisit le vide en évaluant, pour chaque vide, une combinaison pondérée qui varie de manière linéaire et positive par rapport à une mesure de la surface du vide ou des parties de celui-ci; de manière négative par rapport à une mesure de la distance horizontale entre le vide et un emplacement préféré; et de manière quadratique par rapport à une mesure de la distance verticale entre la base du vide et un emplacement préféré, la combinaison varie aussi par rapport à la grandeur et à l'emplacement des autres vides. Un point de pivot est choisi au centre de gravité du vide ou à une partie plus basse de celui-ci. La ligne de taille passe par le point de pivot et sa pente est établie par rapport à une combinaison pondérée de l'effet de la forme et de la grandeur du vide et de la forme et de la grandeur du cal. La combinaison pondérée varie aussi de manière quadratique par rapport à la pente d'une ligne supposée de taille passant par le vide et la pente du cal.
PCT/GB1991/001043 1990-06-28 1991-06-27 Procedes et appareils relatifs a la micropropagation Ceased WO1992000004A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9014387.6 1990-06-28
GB909014387A GB9014387D0 (en) 1990-06-28 1990-06-28 Method and apparatus relating to micropropagation

Publications (1)

Publication Number Publication Date
WO1992000004A1 true WO1992000004A1 (fr) 1992-01-09

Family

ID=10678353

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1991/001043 Ceased WO1992000004A1 (fr) 1990-06-28 1991-06-27 Procedes et appareils relatifs a la micropropagation

Country Status (4)

Country Link
EP (1) EP0536287A1 (fr)
AU (1) AU8198891A (fr)
GB (2) GB9014387D0 (fr)
WO (1) WO1992000004A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5439490A (en) * 1991-02-01 1995-08-08 Plant Production Systems B.V. Method for use in a multiplication process of plants and a device for carrying out said method
US5472038A (en) * 1991-03-07 1995-12-05 Osprey Metals Limited Production of spray deposits
EP3493665B1 (fr) 2016-08-03 2023-08-23 RoBoTec PTC GmbH Dispositif et procédé de reproduction de plantes

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0018861A1 (fr) * 1979-04-11 1980-11-12 Service D'exploitation Industrielle Des Tabacs Et Des Allumettes Procédé et dispositif de saisie sous forme numérique d'une information vidéo représentative d'un produit en feuille
US4285258A (en) * 1978-05-02 1981-08-25 Gulf & Western Corporation Device for translating and rotating a cutting platen with respect to a reciprocal cutter
WO1986006576A1 (fr) * 1985-05-15 1986-11-20 The Commonwealth Industrial Gases Limited Procede et appareil de division de materiaux vegetaux

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4285258A (en) * 1978-05-02 1981-08-25 Gulf & Western Corporation Device for translating and rotating a cutting platen with respect to a reciprocal cutter
EP0018861A1 (fr) * 1979-04-11 1980-11-12 Service D'exploitation Industrielle Des Tabacs Et Des Allumettes Procédé et dispositif de saisie sous forme numérique d'une information vidéo représentative d'un produit en feuille
WO1986006576A1 (fr) * 1985-05-15 1986-11-20 The Commonwealth Industrial Gases Limited Procede et appareil de division de materiaux vegetaux

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5439490A (en) * 1991-02-01 1995-08-08 Plant Production Systems B.V. Method for use in a multiplication process of plants and a device for carrying out said method
EP0570473B1 (fr) * 1991-02-01 1996-01-03 Plant Production Systems B.V. Procede s'utilisant dans un processus de multiplication de plantes et dispositif de mise en application dudit procede
US5472038A (en) * 1991-03-07 1995-12-05 Osprey Metals Limited Production of spray deposits
EP3493665B1 (fr) 2016-08-03 2023-08-23 RoBoTec PTC GmbH Dispositif et procédé de reproduction de plantes

Also Published As

Publication number Publication date
EP0536287A1 (fr) 1993-04-14
GB9014387D0 (en) 1990-08-22
GB9113891D0 (en) 1991-08-14
AU8198891A (en) 1992-01-23
GB2245810A (en) 1992-01-15

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