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WO1990011363A1 - Compositions polysaccharides - Google Patents

Compositions polysaccharides Download PDF

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Publication number
WO1990011363A1
WO1990011363A1 PCT/GB1990/000458 GB9000458W WO9011363A1 WO 1990011363 A1 WO1990011363 A1 WO 1990011363A1 GB 9000458 W GB9000458 W GB 9000458W WO 9011363 A1 WO9011363 A1 WO 9011363A1
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WO
WIPO (PCT)
Prior art keywords
acetan
ingredients
composition
composition according
βdglc
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB1990/000458
Other languages
English (en)
Inventor
Victor John Morris
Barry James Herbert Stevens
Jane Elizabeth Harris
Geoffrey John Brownsey
Allan Patrick Gunning
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biotechnology and Biological Sciences Research Council
Original Assignee
Biotechnology and Biological Sciences Research Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotechnology and Biological Sciences Research Council filed Critical Biotechnology and Biological Sciences Research Council
Publication of WO1990011363A1 publication Critical patent/WO1990011363A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
    • A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
    • A23G3/346—Finished or semi-finished products in the form of powders, paste or liquids
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/60—Salad dressings; Mayonnaise; Ketchup
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/269—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of microbial origin, e.g. xanthan or dextran
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
    • C09D5/00—Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced; Filling pastes
    • C09D5/04—Thixotropic paints
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09G—POLISHING COMPOSITIONS; SKI WAXES
    • C09G1/00—Polishing compositions
    • C09G1/04—Aqueous dispersions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G2200/00—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF containing organic compounds, e.g. synthetic flavouring agents
    • A23G2200/06—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF containing organic compounds, e.g. synthetic flavouring agents containing beet sugar or cane sugar if specifically mentioned or containing other carbohydrates, e.g. starches, gums, alcohol sugar, polysaccharides, dextrin or containing high or low amount of carbohydrate

Definitions

  • the present invention relates to polysaccharide compositions, particularly to polysaccharides from bacteria and polysaccharides for food use.
  • polysaccharide compounds are used in industry, particularly the food industry, for thickening or gelling compositions.
  • Such compounds include starch, pectin, alginate, carrageenans, galactomannans and xanthan gum.
  • a more recently disclosed compound is acetan, the isolation and partial characterisation of which are given in Tayama je aJL, Aqric. Biol. Chem. 49(4), 959-966 (1985) and Couso et al, J. Gen. Microbiol. 133, 2123-2135 (1987).
  • K54 capsular polysaccharide secreted by lebsiella aerogenes serotype K54 has the same carbohydrate structure as the extracellular polysaccharide produced by an Enterobacter species (NCIB 11870) and called XM6. This structure is shown
  • acetan was suitable for food and other uses, as a thixotropic thickening and suspending agen .
  • one aspect of the present invention provides an industrially useful composition
  • an industrially useful composition comprising an aqueous dispersion of acetan and other ingredients, wherein the composition is thixotropic at least partly due to the presence of the acetan.
  • industrially useful composition we mean a manufactured product, particularly produced in bulk, intended for and suitable for sale or for use in an industrial process.
  • compositions include: those with suspended abrasive particles; adhesives; herbicides, pesticides, fertilizers and fungicides; glazes for ceramics; cleaners, such as toilet cleaners and bleaches of the "Domestos" type ("Domestos" is a Registered Trade Mark); emulsions such as toothpastes; foodstuffs such as sauces, salad dressings, ice ⁇ creams, chilled desserts and instant soups; water-based inks; mineral ore suspensions; non-drip paints, rheology modifiers for paper-making; oil-drilling fluids; pigments, for example for textile printing or wall-paper printing; shoe, furniture and metal polishes; and welding rods.
  • Domestos is a Registered Trade Mark
  • emulsions such as toothpastes
  • foodstuffs such as sauces, salad dressings, ice ⁇ creams, chilled desserts and instant soups
  • water-based inks mineral ore suspensions
  • Acetan is the trivial name given to the extracellular polysaccharide (EPS) secreted by Acetobacter xylinium.
  • EPS extracellular polysaccharide
  • Structure II is the latest and perhaps best structure to date.
  • the ano eric configurations (alpha, beta etc) will require checking and this may explain the apparent difference between I and II.
  • Structure I is claimed to contain one O-acetyl substituent per repeat unit and structure II has one-two O-acetyl groups per repeat unit.
  • the references above define the polysaccharide.
  • the CTAB preparation described in Tayama et al_ is probably the best method of purification. Once purified, the polymer should contain the following sugars: glucose (DGlc), mannose (DMan), glucuronic acid (DGlcA) and rhamnose (LRha) in the ratio
  • Acetan may be prepared from at least some strains of the organism Acetobacter xylinium (including the strains deposited as an open deposit with the U.S. Dept. of Agriculture's Agricultural Research Culture Collection, Peoria, Illinois, as NRRL B42; and with the National Collections of Industrial and Marine Bacteria on 20th March 1989 as deposit no NCIB 40123, the latter deposit having been made under the provisions of the Budapest Treaty for the purposes of this patent application) in the manner described hereinafter, by the methods of Tayama et a_l or by the methods of Couso et al_.
  • the acetan can be precipitated with ethanol, washed and freeze-dried for storage.
  • Other polymers may co-precipitate with the acetan. These do not usually affect the porperties of the acetan, but may of course dilute it.
  • Acetan may be used in industrially useful compositions in ways which are analogous to those involving xanthan.
  • concentration of acetan may be from 0.1% to 5%.
  • the pH of the composition is preferably between 2.5 and 10, for the thixotropic effect to be achieved optimally. It is surprising that acetan solutions remain viscous at pH's below 5.
  • Such compositions form a preferred aspect of the invention, and include acidic vinegar based sauces .
  • the salt concentration is between 0.01M and 2M.
  • salt we mean inorganic salts of strong or weak acids preferably containing monovalent or divalent cations and a soluble anion.
  • acetan can be used in a freeze-dried form.
  • suitably treated broths from A.xylinium fermentations can also be used.
  • the untreated bifbtfes of most strains suffer two immediate disadvantages. Firstly the broths contain a cellulose-based pellicle structure which fo ⁇ is at the top of the growth vessel and secondly the broths are coloured and odorous. The cellulose pellicles can be removed by filtration. The colour and odour present in the broths can be completely removed by treatment with charcoal.
  • the broths can be filtered through a charcoal column, or granular charcoal can be added to the broth and then removed by filtration.
  • a further aspect of the invention provides a broth from the fermentation of Acetobacter xylinium which has been treated (a) to remove the pellicle without removing the acetan and/or (b) by substantial removal of colour and odour.
  • the extent to which the pellicle, colour and odour must be removed will depend upon the use to which the acetan broth is to be put; generally, though, complete removal of the pellicle is desirable.
  • the concentration of acetan in the broth usually needs to be concentrated, for example by rotary evaporation, before use.
  • a concentration of at least 1.0 - 2.0% is generally useful and is, in fact, high enough to permit subsequently blending with vinegar, water or the like, whilst still yielding a useful concentration of acetan in the final product. Above 3%, the dispersion becomes very viscous and cannot be concentrated further by rotary evaporation.
  • Some strains of A. ylinium are naturally “pellicle-minus", at least when in a shaken culture which reduces the formation of pellicle even with the more common strains of A. ylinium.
  • the yield of acetan can be increased with such "pellicle-minus" strains, at least when grown in shaken culture.
  • Figure 1 is a viscosity/shear rate graph for varying strengths of aqueous acetan dispersions.
  • Acetan is prepared from cultures of Acetobacter xylinum NRRL B42 grown in AJ medium.
  • AJ medium contains (g/1): yeast extract (Difco) (10); glucose (50); 2HP04 (0.1); KH2P04 (0.1); MgS04.7H20 (0.25); FeC13 (0.005).
  • the pH of the medium is adjusted to 6.0.
  • Cultures are grown at 25oC for 7 days with shaking to ensure aeration. Cells are removed from the culture medium by centrifugation (23,000g, 30 ins). If necessary, the medium is diluted with water to facilitate sedimentation of the cells. Remaining cells may be removed by passage through a Millipore filter (pore size 0.65um).
  • the cell-free culture medium is then concentrated by rotary evaporation at 40oC to approximately half the original volume, and solid KC1 is added to a final concentration of 1% (w/v) .
  • Acetan is precipitated from the medium with 2 volumes of industrial spirit. The precipitate is coarse and fibrous, and easily separated from the liquid. Acetan is washed twice with industrial spirit, and excess liquid squeezed out before re- dissolving in glass distilled water and freeze-drying.
  • Acetan was prepared as in Example 1, except that the rotary evaporation was continued for longer, resulting in a highly visco-elastic liquid, from which the acetan was not precipitated. Differing strength of aqueous dispersions of acetan prepared according to Example 1 were tested in standard apparatus to yield the viscosity/shear rate data for Figure 1. Note the coincidence of the data, for each dispersion, whether the shear rate is being increased or decreased.
  • acetan is included to give the composition the desired physical properties. Glycerine may be employed as well. If too thick, add more water. If the action is too slow, up to 5% of sodium fluoride may be added. Use in a hood or well ventilated room.
  • Example 5 Stabiliser for Particles or Emulsions in Food
  • Acetan is used to suspend particles or droplets.
  • Acetan may be used to stabilise dispersions of dried whey or heat-processed soya protein in water.
  • Example 9 Silver Polish
  • the grease consists of 50% oleic or stearic or palmitic acid, 10% Triton XII and 40% Tallow mineral oil).
  • Example 12 Liquid Feed Supplement containing added suspended limestone

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Zoology (AREA)
  • Dispersion Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Birds (AREA)
  • Materials Engineering (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • General Chemical & Material Sciences (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cosmetics (AREA)

Abstract

L'acétane est un polysaccharide extracellulaire que sécrète l'Acetobacter xylinium. On décrit des dispersions aqueuses thixotropiques utiles dans des compositions telles que les aliments, les adhésifs, les produits de nettoyage, les pâtes dentifrices et les peintures.
PCT/GB1990/000458 1989-03-29 1990-03-27 Compositions polysaccharides Ceased WO1990011363A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB8907057.7 1989-03-29
GB898907057A GB8907057D0 (en) 1989-03-29 1989-03-29 Polysaccharide compositions

Publications (1)

Publication Number Publication Date
WO1990011363A1 true WO1990011363A1 (fr) 1990-10-04

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PCT/GB1990/000458 Ceased WO1990011363A1 (fr) 1989-03-29 1990-03-27 Compositions polysaccharides

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8343286B2 (en) 2008-12-02 2013-01-01 Diversey, Inc. Ware washing system containing cationic starch
US8647444B2 (en) 2007-07-05 2014-02-11 Diversey, Inc. Rinse aid
US8876982B2 (en) 2005-05-04 2014-11-04 Diversey, Inc. Warewashing method using a cleaning composition containing low levels of surfactant
US11643559B2 (en) 2017-07-25 2023-05-09 Magnomer, Inc. Methods and compositions for magnetizable plastics
US12478979B2 (en) 2019-01-30 2025-11-25 Magnomer, Inc. Methods and compositions for magnetizable plastics

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0127698A1 (fr) * 1983-05-02 1984-12-12 Nakano Vinegar Co., Ltd. Hétéropolysaccharide acide AM-2, procédé pour sa préparation et ses usages, acétobacter MH-1597 (FERM BP-280)
JPS60105460A (ja) * 1983-11-14 1985-06-10 Nakano Vinegar Co Ltd 食品用増粘・乳化剤

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0127698A1 (fr) * 1983-05-02 1984-12-12 Nakano Vinegar Co., Ltd. Hétéropolysaccharide acide AM-2, procédé pour sa préparation et ses usages, acétobacter MH-1597 (FERM BP-280)
JPS60105460A (ja) * 1983-11-14 1985-06-10 Nakano Vinegar Co Ltd 食品用増粘・乳化剤

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, Volume 109, No. 1, 4 July 1988, (Columbus, Ohio, US), H. MASAI: "Utilization of Products of Acetic Acid Bacteria in the Food Industry", see page 511* Abstract 5330m, & Nippon Shokuhin Kogyo Gakkaishi 1988, 35(2), 126-32* *
PATENT ABSTRACTS OF JAPAN, Volume 9, No. 247 (C-307) (1970), 3 October 1985; & JP-A-60105460 (Nakano Suten K.K.) 10 Juni 1985 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8876982B2 (en) 2005-05-04 2014-11-04 Diversey, Inc. Warewashing method using a cleaning composition containing low levels of surfactant
US8647444B2 (en) 2007-07-05 2014-02-11 Diversey, Inc. Rinse aid
US8343286B2 (en) 2008-12-02 2013-01-01 Diversey, Inc. Ware washing system containing cationic starch
US11643559B2 (en) 2017-07-25 2023-05-09 Magnomer, Inc. Methods and compositions for magnetizable plastics
US12209190B2 (en) 2017-07-25 2025-01-28 Magnomer, Inc. Methods and compositions for magnetizable plastics
US12478979B2 (en) 2019-01-30 2025-11-25 Magnomer, Inc. Methods and compositions for magnetizable plastics

Also Published As

Publication number Publication date
GB8907057D0 (en) 1989-05-10

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