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WO1990007716A1 - Tube d'inspection immunologique - Google Patents

Tube d'inspection immunologique Download PDF

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Publication number
WO1990007716A1
WO1990007716A1 PCT/JP1988/001297 JP8801297W WO9007716A1 WO 1990007716 A1 WO1990007716 A1 WO 1990007716A1 JP 8801297 W JP8801297 W JP 8801297W WO 9007716 A1 WO9007716 A1 WO 9007716A1
Authority
WO
WIPO (PCT)
Prior art keywords
test tube
immunological
tube according
immunological test
substance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP1988/001297
Other languages
English (en)
Japanese (ja)
Inventor
Yasuo Murao
Shuntaro Hosaka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toray Industries Inc
Original Assignee
Toray Industries Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toray Industries Inc filed Critical Toray Industries Inc
Priority to PCT/JP1988/001297 priority Critical patent/WO1990007716A1/fr
Publication of WO1990007716A1 publication Critical patent/WO1990007716A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G01N33/5304—Reaction vessels, e.g. agglutination plates

Definitions

  • the present invention relates to a test tube for performing an immunological agglutination reaction for the purpose of detecting an immunologically active substance such as an antigen or an antibody.
  • the substance that binds to the substance to be detected is immobilized on carrier particles of an appropriate size, and the particles are Utilizing the phenomenon of aggregation in the presence of the target substance, a method of detecting the target substance with high sensitivity is an important means of immunological clinical tests.
  • the visual determination of whether or not immunologically active substance-immobilized particles cause an immunological agglutination reaction is mainly performed by the following method.
  • One is a method in which a sample solution and a reagent for agglutination reaction are mixed and shaken on a plate, and after a few minutes, judgment is made based on whether or not an aggregate is observed.
  • Another method is to mix the sample solution and the reagent for agglutination in a microtiter plate and observe the sedimentation image of the reagent for agglutination after a few hours. is there.
  • each of the conventional methods has some problems.
  • the method of observing the presence or absence of agglomerates on a plate can be determined in a short time, but since drying is fast, the next test is performed after the determination is completed, so it is inefficient to test a large number of samples.
  • the aggregate itself is observed with the naked eye, the sensitivity is low.
  • the method of observing the sedimentation image of the agglutination reagent in a microtiter plate is highly sensitive but requires several hours to make a determination.
  • both methods require a process of collecting the sample liquid and adding a separately prepared agglutination reagent.
  • the inventors of the present invention used a freeze-dried and charged agglutination reagent and a sample liquid in a transparent tube described in JP-A-58-73666. After mixing in a tube, the mixture was allowed to stand horizontally, and after several tens of minutes, a method of observing the sedimentation image of the agglutination reagent and making a determination was invented. However, if the transparent tube used in this method has a uniform diameter, the freeze-dried agglutination reagent falls off the tube with a slight impact, and when the sample solution is inhaled, the agglutination reagent is used. Partially leaks out of the pipe. An object of the present invention is to solve such a problem and to provide the most advantageous immunological reaction test tube.
  • the present invention is an immunological test tube comprising a transparent tube having at least one end fitted with a passage, and further containing a freeze-dried immunological agglutination reagent in the tube.
  • FIG. 1 shows a preferred embodiment of the present invention, wherein 1 is a pitta, and 2 is an agglutination reagent.
  • FIG. 2 shows the inspection results of the example, in which (a) shows the (1) image and (b) shows the (+) image.
  • the material of the test tube of the present invention may be plastic or glass such as polystyrene, polyethylene, polypropylene, polycarbonate, polyvinyl chloride, and polymethyl methacrylate.
  • plastic or glass such as polystyrene, polyethylene, polypropylene, polycarbonate, polyvinyl chloride, and polymethyl methacrylate.
  • polystyrene and glass are particularly preferable.
  • the diameter of the test tube is preferably 1 to 5 dragons in inner diameter, particularly preferably about 2 inner diameters.
  • the length of the tube is preferably 1 to 10 cm, more preferably 3 to 5 cm.
  • Means for narrowing the passage include narrowing the tube or packing or bonding a porous substance such as absorbent cotton or a filter, but the thinner tube is preferable.
  • the inner diameter of the tip should be 0.3 to 1. It is preferable that the thickness be reduced to five, particularly about 1 mm.
  • the agglutination reagent may be charged in advance by freeze-drying or may be charged by freeze-drying in a tube, but it is preferable to charge the reagent in the vicinity of the tight tip in the tube.
  • the agglutination reagent here refers to a substance that specifically binds to a substance to be measured or a substance to be measured immobilized on a carrier.
  • the substance to be immobilized on these carriers is not particularly limited, and specifically, hepatitis (types A and B) virus, AIDS virus (HIV-IE), ATL virus (HIV-I), and herpes (herpes) Simplex, Brown zela soster) virus, cytome garovirus, measles virus, rubella virus, polio-mauizores, no.
  • Viruses such as Lactobacillus botulinum, Botulinum, Pulcella, Shigella, Vibrio parahaemolyticus, Pest, Escherichia coli, Campylobacter, Gangsita, Toxoplasma, Malaria parasite, and Trebonemapari dam.
  • antigens derived from microorganisms such as bacteria, fungi, and protozoa, C-reactive protein, carcinoembryonic antigen, --futoprotein, human chorionic gonadotropin, heat-aggregated immunoglobulin G, hemoglobin, nucleoprotein, nucleic acid , Estrogen, complement components, strepturidin 0, etc., or substances such as antibodies against them.
  • These substances include blood cells (fixed with formalin, etc.), bacterial cells, polystyrene latex, (meta) acrylonitrile-based polymers, and (meta) acrylic acid esters. It is immobilized on a carrier such as a polymer as a main component or other polymer micro beads by adsorption, ionic bond or covalent bond.
  • the test tube of the present invention has a cap-shaped or cylindrical pipe made of elastic rubber, plastic, or the like, at the end where no reagent is charged in order to aspirate the sample liquid into the tube. Or, preferably, have a piston. In addition, an existing micropitter or the like may be connected and used. Alternatively, the sample solution may be injected using another pipet.
  • FIG. 1 A preferred example of the present invention is shown in FIG. 1
  • the test tube After inhaling or injecting the sample liquid, the test tube is placed horizontally, but if it is rolled in the meantime, it will disturb the sedimentation image of the agglutination reagent, which is not desirable.
  • a predetermined amount of a sample solution is inhaled or injected into the tube, and the prepared agglutination reagent and the sample solution are mixed well and left horizontally for several tens of minutes. After that, the presence or absence of agglutination is examined by observing the sedimentation image of the agglutination reagent.
  • the BSA-immobilized polymer microbead dispersion (0.63%) 20 ⁇ described in No. 1,562,113 was searched for and freeze-dried. After inhaling 20 d of a phosphate buffered saline (PBS) solution of 200 g Ab / ml of anti-BS ⁇ serum (rabbit) in the accompanying pipe, the tube is moved up and down or in the tube axis direction. By rotating the mixture, the BSA-immobilized polymer microbeads were mixed well and allowed to stand on a horizontal surface.
  • PBS phosphate buffered saline
  • anti-BSA antiserum (rabbit) was used by diluting the same ratio with BSA PBS solution (BSA 1 mg / ml). After 30 minutes, the results are shown in FIG. Fig. 2 shows the results when viewed from above the test tube.
  • the negative control to which BSA was added shows the (a) image of (a), and the negative control without BSA shows the (b) image. (+) Image was shown.
  • Example 2 In the same manner as in Example 2, a dispersion of polymer microbeads on which an anti-human hemoglobin antibody was immobilized was freeze-dried.
  • the human hemoglobin 0.1 ⁇ g Zml PBS solution 20 was inhaled, the same operation as in Example 1 was performed, and the mixture was allowed to stand on a horizontal surface. PBS was used as a negative control. Judging after 30 minutes, the human hemoglobin ⁇ .1 g Zml PBS solution showed the (+) image in FIG. 2 and the PBS solution showed the (-) image in FIG.
  • the present invention it is possible to stably hold an agglutination reagent prepared by freeze-drying,
  • the sample solution can be inhaled or injected without the reagent flowing out of the tube, and the agglutination reagent can be mixed at the same time as the sample solution is collected, which simplifies the operation. In addition, quick judgment is possible with tens of minutes.
  • the present invention is useful as a test tube for performing an immunological agglutination reaction for the purpose of detecting an immunoreactive substance such as an antigen or an antibody.

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

On peut évaluer une agglutination grâce à une procédure simple ne présentant pas d'inconvénients tels que le débordement d'un réactif d'agglutination, par l'utilisation d'un tube d'inspection immunologique d'agglutination composé d'un tube transparent présentant un chemin dont au moins l'une des extrémités est rétrécie.
PCT/JP1988/001297 1988-12-23 1988-12-23 Tube d'inspection immunologique Ceased WO1990007716A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/JP1988/001297 WO1990007716A1 (fr) 1988-12-23 1988-12-23 Tube d'inspection immunologique

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP1988/001297 WO1990007716A1 (fr) 1988-12-23 1988-12-23 Tube d'inspection immunologique

Publications (1)

Publication Number Publication Date
WO1990007716A1 true WO1990007716A1 (fr) 1990-07-12

Family

ID=13930942

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP1988/001297 Ceased WO1990007716A1 (fr) 1988-12-23 1988-12-23 Tube d'inspection immunologique

Country Status (1)

Country Link
WO (1) WO1990007716A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4117920Y1 (fr) * 1964-03-09 1966-08-19
JPS5873866A (ja) * 1981-10-28 1983-05-04 Toray Ind Inc 免疫学的検査方法
JPS61213770A (ja) * 1985-03-20 1986-09-22 Toyobo Co Ltd ラテックス凝集反応測定用装置

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4117920Y1 (fr) * 1964-03-09 1966-08-19
JPS5873866A (ja) * 1981-10-28 1983-05-04 Toray Ind Inc 免疫学的検査方法
JPS61213770A (ja) * 1985-03-20 1986-09-22 Toyobo Co Ltd ラテックス凝集反応測定用装置

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