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WO1988001298A3 - Procede a membrane enzymatique de synthese et de separation de peptides - Google Patents

Procede a membrane enzymatique de synthese et de separation de peptides Download PDF

Info

Publication number
WO1988001298A3
WO1988001298A3 PCT/US1987/002030 US8702030W WO8801298A3 WO 1988001298 A3 WO1988001298 A3 WO 1988001298A3 US 8702030 W US8702030 W US 8702030W WO 8801298 A3 WO8801298 A3 WO 8801298A3
Authority
WO
WIPO (PCT)
Prior art keywords
uncharged
reaction mixture
peptide
membrane
product
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1987/002030
Other languages
English (en)
Other versions
WO1988001298A2 (fr
Inventor
Guillermo A Jacobucci
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Coca Cola Co
Original Assignee
Coca Cola Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/897,679 external-priority patent/US5037741A/en
Priority to AT87905545T priority Critical patent/ATE102654T1/de
Priority to DE3789309T priority patent/DE3789309T2/de
Priority to KR1019880700343A priority patent/KR900004070B1/ko
Application filed by Coca Cola Co filed Critical Coca Cola Co
Publication of WO1988001298A2 publication Critical patent/WO1988001298A2/fr
Publication of WO1988001298A3 publication Critical patent/WO1988001298A3/fr
Priority to DK209388A priority patent/DK209388D0/da
Priority to NO881669A priority patent/NO172064C/no
Priority to FI884359A priority patent/FI884359A0/fi
Priority to GB8903271A priority patent/GB2218991B/en
Anticipated expiration legal-status Critical
Priority to SG35491A priority patent/SG35491G/en
Priority to HK50691A priority patent/HK50691A/xx
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06104—Dipeptides with the first amino acid being acidic
    • C07K5/06113—Asp- or Asn-amino acid
    • C07K5/06121—Asp- or Asn-amino acid the second amino acid being aromatic or cycloaliphatic
    • C07K5/0613—Aspartame
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/813—Continuous fermentation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/819—Fermentation vessels in series

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

la présente invention permet de résoudre les problèmes dus à la précipitation d'un composé intermédiaire et/ou à la désactivation rapide d'enzymes associés à des procédés connus. Un procédé de synthèse enzymatique à membrane de peptides consiste à altérer l'équilibre chimique existant dans un mélange de réaction entre des acides aminés réactifs chargés ou ionisés et des produits peptidiques non-chargés ou non-ionisés en présence d'une enzyme protéolytique telle que la thermolysine. L'équilibre est altéré par diffusion du produit peptidique non chargé à travers une membrane de rejet d'ions qui enlève les peptides non chargés du mélange réactif, le peptide non chargé ainsi diffusé étant de préférence rapidement converti en une espèce chargée qui ne peut pas revenir par diffusion au mélange réctif, de sorte que les peptides non chargés sont pratiquement ''aspirés'' à travers la membrane. La conversion enzymatique de l'espèce non chargée utilise une estérase telle que l'aminoacylase I. On peut séparer des réactifs coperméables du mélange de produit et les retourner au mélange réactif. De même, la membrane de rejet d'ions peut être utilisée pour résoudre des énantiomères d'acides carboxyliques racémiques, y compris des acides aminés D, L.
PCT/US1987/002030 1986-08-18 1987-08-14 Procede a membrane enzymatique de synthese et de separation de peptides Ceased WO1988001298A2 (fr)

Priority Applications (9)

Application Number Priority Date Filing Date Title
AT87905545T ATE102654T1 (de) 1986-08-18 1987-08-14 Enzymatische membran, verfahren zur herstellung und trennung von peptiden.
DE3789309T DE3789309T2 (de) 1986-08-18 1987-08-14 Enzymatische membran, verfahren zur herstellung und trennung von peptiden.
KR1019880700343A KR900004070B1 (ko) 1986-08-18 1987-08-14 펩티드의 합성 및 분리를 위한 효소막법
DK209388A DK209388D0 (da) 1986-08-18 1988-04-15 Fremgangsmaade til enzymatisk syntese af peptider eller til enzymatisk spaltning af racemiske carboxylsyrer
NO881669A NO172064C (no) 1986-08-18 1988-04-18 Fremgangsmaate for enzymatisk syntese av peptider og fremgangsmaate for enzymatisk separering av racemiske karboksylsyreforbindelser
FI884359A FI884359A0 (fi) 1986-08-18 1988-09-22 Enzymatiskt membranfoerfarande foer syntes och separation av peptider.
GB8903271A GB2218991B (en) 1986-08-18 1989-02-14 Enzymatic membrane method for the synthesis of peptides and the separation of optical isomers
SG35491A SG35491G (en) 1986-08-18 1991-05-09 Enzymatic membrane method for the synthesis of peptides and the separation of optical isomers
HK50691A HK50691A (en) 1986-08-18 1991-07-04 Enzymatic membrane method for the synthesis of peptides and the separation of optical isomers

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US06/897,679 US5037741A (en) 1986-08-18 1986-08-18 Enzymatic method for the synthesis and separation of peptides
US897,679 1986-08-18
US07/078,504 US5002871A (en) 1986-08-18 1987-07-28 Enzymatic membrane method for the synthesis and separation of peptides
US078,504 1987-07-28

Publications (2)

Publication Number Publication Date
WO1988001298A2 WO1988001298A2 (fr) 1988-02-25
WO1988001298A3 true WO1988001298A3 (fr) 1988-04-07

Family

ID=26760616

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1987/002030 Ceased WO1988001298A2 (fr) 1986-08-18 1987-08-14 Procede a membrane enzymatique de synthese et de separation de peptides

Country Status (22)

Country Link
US (1) US5002871A (fr)
EP (1) EP0321479B1 (fr)
JP (1) JPH06319592A (fr)
KR (1) KR900004070B1 (fr)
CN (1) CN1026498C (fr)
AR (1) AR242264A1 (fr)
AT (1) ATE102654T1 (fr)
AU (1) AU610520B2 (fr)
DE (1) DE3789309T2 (fr)
DK (1) DK209388D0 (fr)
EG (1) EG18454A (fr)
ES (1) ES2007103A6 (fr)
FI (1) FI884359A0 (fr)
GB (1) GB2218991B (fr)
GR (1) GR871290B (fr)
IL (2) IL83564A (fr)
MX (1) MX168598B (fr)
NZ (2) NZ231657A (fr)
PH (1) PH26764A (fr)
PT (1) PT85548B (fr)
SG (1) SG35491G (fr)
WO (1) WO1988001298A2 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5244791A (en) * 1984-05-29 1993-09-14 Genecor International, Inc. Methods of ester hydrolysis
US5055399A (en) * 1985-01-15 1991-10-08 Genentech, Inc. Enzymatic synthesis of alpha-L-aspartyl-L-phenyalanine lower alkyl esters
US5336601A (en) * 1986-08-18 1994-08-09 The Coca-Cola Company Enzymatic membrane method for the snythesis and separation of peptides
US5202235A (en) * 1986-08-18 1993-04-13 The Coca-Cola Company Enzymatic method for the synthesis and separation of peptides
US5350681A (en) * 1986-08-18 1994-09-27 The Coca-Cola Company Enzymatic membrane method for the synthesis and separation of peptides
US4800162A (en) * 1987-04-01 1989-01-24 Sepracor, Inc. Method for resolution of steroisomers in multiphase and extractive membrane reactors
CA2054754A1 (fr) * 1989-04-24 1990-10-25 Daniel J. Brose Methode enzymatique faisant appel a une membrane pour la synthese et la separation de peptides
US5314807A (en) * 1991-03-29 1994-05-24 Nippon Gohsei Kagaku Kogyo Kabushiki Kaisha Method for producing an angiotensin converting enzyme inhibitor-containing composition
WO1992018516A1 (fr) * 1991-04-12 1992-10-29 The Children's Hospital Of Philadelphia Nouvelle endopeptidase
WO1998033581A1 (fr) * 1997-02-04 1998-08-06 Akzo Nobel N.V. Module a fibres creuses d'au moins deux groupes, et mode de fabrication
FR2777803B1 (fr) * 1998-04-24 2000-07-28 Univ Claude Bernard Lyon Procede d'elimination active et selective de petites molecules par pompage enzymatique : dialyse active
US6500571B2 (en) 1998-08-19 2002-12-31 Powerzyme, Inc. Enzymatic fuel cell
EP1311345A1 (fr) * 2000-04-21 2003-05-21 University of Rochester Cuve a reaction biphasique et procedes d'utilisation
US6485650B1 (en) * 2000-08-28 2002-11-26 Facilichem, Inc. Liquid membrane separation of enantiomers
US20020114906A1 (en) * 2000-11-20 2002-08-22 Bridgestone Corporation Base body for photosensitive drum and photosensitive drum using the same
CN117820436B (zh) * 2023-03-24 2024-09-24 烟台瀚海同仁生物技术研发有限公司 降血糖抗氧化保护肾脏的菊芋肽、制备方法及其在大健康中的应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0017485A1 (fr) * 1979-04-06 1980-10-15 De Forenede Bryggerier A/S Procédé de production enzymatique de peptides
US4251631A (en) * 1978-02-23 1981-02-17 Research Products Rehovot Ltd. Cross-linked enzyme membrane
EP0121265A2 (fr) * 1983-04-04 1984-10-10 Nippon Oil And Fats Company, Limited Procédé de réaction en phase hétérogène
EP0124313A2 (fr) * 1983-04-28 1984-11-07 Ajinomoto Co., Inc. Procédé pour la préparation de l'ester méthylique de la L-aspartyl-L-phénylalanine ou de la L-aspartyl-L-phénylalanine
EP0188342A2 (fr) * 1985-01-15 1986-07-23 Genentech, Inc. Méthode et appareil pour synthèse enzymatique

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL60227C (fr) * 1943-02-23
US3625734A (en) * 1969-10-16 1971-12-07 Usa Ultra-thin liquid membrane construction
US3779907A (en) * 1970-04-13 1973-12-18 Exxon Research Engineering Co Liquid membrane process for the separation of aqueous mixtures
CA947214A (en) * 1971-04-02 1974-05-14 Antoine D'iorio Resolution of racemates of ring-substituted phenylalanines
US3933781A (en) * 1973-11-05 1976-01-20 Monsanto Company Process for the preparation of α-L-aspartyl-L-phenylalanine alkyl esters
US4119493A (en) * 1975-10-23 1978-10-10 (Zaidanhojin) Sagami Chemical Research Center Process for producing a peptide
DE2804892A1 (de) * 1977-02-09 1978-08-10 Procter & Gamble Verfahren zur herstellung von l-threonin und dessen n-acylderivat
US4187086A (en) * 1977-06-15 1980-02-05 General Electric Company Packaged membrane system and replenishment method
US4119408A (en) * 1977-06-22 1978-10-10 General Electric Company Apparatus for maintaining the separation efficiency of immobilized liquid membranes in gas separation
GB2047564B (en) * 1978-03-27 1983-01-26 Bend Res Inc Separator membrane and process using such membrane for removing ions from an aqueous solution
DE2907450A1 (de) * 1979-02-26 1980-09-04 Rehovot Res Prod Verfahren zur trennung eines waessrigen loesungsgemisches durch elektrodialyse
DE3274985D1 (en) * 1981-09-21 1987-02-12 Toyo Soda Mfg Co Ltd Process for recovering a dipeptide derivative
US4455210A (en) * 1982-03-04 1984-06-19 General Electric Company Multi layer ion exchanging membrane with protected interior hydroxyl ion rejection layer
JPS60164495A (ja) * 1984-01-16 1985-08-27 モンサント　コンパニー Ｎ‐ホルミルアミノ酸とペプチド残基の酵素的結合法
DE3438189A1 (de) * 1984-10-18 1986-04-24 Hoechst Ag, 6230 Frankfurt Verfahren zur herstellung aromatisch substituierter l-aminosaeuren
US4743547A (en) * 1985-04-09 1988-05-10 Kuraray Co., Ltd. Method of producing L-phenylalanine

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4251631A (en) * 1978-02-23 1981-02-17 Research Products Rehovot Ltd. Cross-linked enzyme membrane
EP0017485A1 (fr) * 1979-04-06 1980-10-15 De Forenede Bryggerier A/S Procédé de production enzymatique de peptides
EP0121265A2 (fr) * 1983-04-04 1984-10-10 Nippon Oil And Fats Company, Limited Procédé de réaction en phase hétérogène
EP0124313A2 (fr) * 1983-04-28 1984-11-07 Ajinomoto Co., Inc. Procédé pour la préparation de l'ester méthylique de la L-aspartyl-L-phénylalanine ou de la L-aspartyl-L-phénylalanine
EP0188342A2 (fr) * 1985-01-15 1986-07-23 Genentech, Inc. Méthode et appareil pour synthèse enzymatique

Also Published As

Publication number Publication date
EP0321479B1 (fr) 1994-03-09
GR871290B (en) 1987-12-23
ATE102654T1 (de) 1994-03-15
GB2218991A (en) 1989-11-29
AU7854387A (en) 1988-03-08
PT85548A (en) 1987-09-01
AU610520B2 (en) 1991-05-23
IL98061A (en) 1992-03-29
DE3789309T2 (de) 1994-10-06
ES2007103A6 (es) 1989-06-01
KR880701778A (ko) 1988-11-05
EP0321479A1 (fr) 1989-06-28
SG35491G (en) 1991-06-21
DK209388A (da) 1988-04-15
KR900004070B1 (ko) 1990-06-11
DK209388D0 (da) 1988-04-15
NZ221467A (en) 1990-07-26
GB8903271D0 (en) 1989-08-23
MX168598B (es) 1993-06-01
CN87106791A (zh) 1988-05-04
WO1988001298A2 (fr) 1988-02-25
IL83564A0 (en) 1988-01-31
NZ231657A (en) 1991-07-26
JPH06319592A (ja) 1994-11-22
GB2218991B (en) 1991-02-13
EG18454A (en) 1994-10-30
PH26764A (en) 1992-09-28
CN1026498C (zh) 1994-11-09
AR242264A1 (es) 1993-03-31
FI884359A7 (fi) 1988-09-22
DE3789309D1 (de) 1994-04-14
FI884359A0 (fi) 1988-09-22
US5002871A (en) 1991-03-26
IL83564A (en) 1992-03-29
PT85548B (pt) 1990-05-31

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