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WO1987000204A3 - Purification de somatotropine a partir de micro-organismes transformes - Google Patents

Purification de somatotropine a partir de micro-organismes transformes

Info

Publication number
WO1987000204A3
WO1987000204A3 PCT/US1986/001289 US8601289W WO8700204A3 WO 1987000204 A3 WO1987000204 A3 WO 1987000204A3 US 8601289 W US8601289 W US 8601289W WO 8700204 A3 WO8700204 A3 WO 8700204A3
Authority
WO
WIPO (PCT)
Prior art keywords
purification
somatotropin
transformed microorganisms
microorganisms
purifying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1986/001289
Other languages
English (en)
Other versions
WO1987000204A2 (fr
Inventor
Timothy W Evans
Mark W Knuth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pharmacia and Upjohn Co
Original Assignee
Upjohn Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25011872&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1987000204(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Upjohn Co filed Critical Upjohn Co
Priority to JP61503479A priority Critical patent/JPH06104070B2/ja
Priority to DE8686904011T priority patent/DE3685424D1/de
Priority to US08/256,297 priority patent/US6916914B1/en
Priority to AT86904011T priority patent/ATE76435T1/de
Publication of WO1987000204A2 publication Critical patent/WO1987000204A2/fr
Publication of WO1987000204A3 publication Critical patent/WO1987000204A3/fr
Anticipated expiration legal-status Critical
Priority to US08/447,381 priority patent/US6586575B1/en
Priority to HK64897A priority patent/HK64897A/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Endocrinology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Processus commercialement avantageux d'extraction et de purification de protéines à partir de micro-organismes. Les étapes initiales du processus sont utiles pour purifier beaucoup de protéines insolubles, alors que les étapes successives sont destinées à régénérer les somatotropines dénaturées produites par les micro-organismes transformés. Ce processus est particulièrement utile dans la purification de somatotropines bovines produites de manière recombinante.
PCT/US1986/001289 1985-06-26 1986-06-13 Purification de somatotropine a partir de micro-organismes transformes Ceased WO1987000204A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP61503479A JPH06104070B2 (ja) 1985-06-26 1986-06-13 形質転換した微生物からのソマトトロピンの精製
DE8686904011T DE3685424D1 (de) 1985-06-26 1986-06-13 Solubilisation und oxidation von somatotropin aus transformierten mikroorganismen.
US08/256,297 US6916914B1 (en) 1985-06-26 1986-06-13 Purification of somatotropin from transformed microorganisms
AT86904011T ATE76435T1 (de) 1985-06-26 1986-06-13 Solubilisation und oxidation von somatotropin aus transformierten mikroorganismen.
US08/447,381 US6586575B1 (en) 1985-06-26 1995-05-23 Purification of somatotropin from transformed microorganisms
HK64897A HK64897A (en) 1985-06-26 1997-05-15 Solubilization and oxidation somatotropin from transformed microorganisms

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US74901685A 1985-06-26 1985-06-26
US749,016 1985-06-26
EP86307769A EP0263902B1 (fr) 1985-06-26 1986-10-08 Solubilisation et oxydation de somatotropine de micro-organismes transformés

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US74901685A Continuation-In-Part 1985-06-26 1985-06-26

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US08/256,297 Continuation-In-Part US6916914B1 (en) 1985-06-26 1986-06-13 Purification of somatotropin from transformed microorganisms

Publications (2)

Publication Number Publication Date
WO1987000204A2 WO1987000204A2 (fr) 1987-01-15
WO1987000204A3 true WO1987000204A3 (fr) 1987-03-12

Family

ID=25011872

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1986/001289 Ceased WO1987000204A2 (fr) 1985-06-26 1986-06-13 Purification de somatotropine a partir de micro-organismes transformes

Country Status (9)

Country Link
US (2) US6916914B1 (fr)
EP (2) EP0229110B1 (fr)
JP (1) JPH06104070B2 (fr)
AT (1) ATE76435T1 (fr)
DE (1) DE3685424D1 (fr)
ES (1) ES2032383T3 (fr)
GR (1) GR3004917T3 (fr)
HK (1) HK64897A (fr)
WO (1) WO1987000204A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3685424D1 (de) * 1985-06-26 1992-06-25 Upjohn Co Solubilisation und oxidation von somatotropin aus transformierten mikroorganismen.
JPH01132598A (ja) * 1987-10-14 1989-05-25 Pitman Moore Inc 変性剤溶液中に含まれる組換え蛋白質における分子内ジスルフィド結合の生成を促進する方法
US4958007A (en) * 1988-05-17 1990-09-18 Schering-Plough Corp. Extraction of human interleukin-4- from bacteria
US5079230A (en) * 1988-09-12 1992-01-07 Pitman-Moore, Inc. Stable bioactive somatotropins
US5023323A (en) * 1990-09-12 1991-06-11 Monsanto Company Method of somatotropin naturation
ZA9711733B (en) 1996-12-31 1998-07-01 Monsanto Co Method for solubilization and naturation of somatotropins
PE20090100A1 (es) * 2007-03-30 2009-02-26 Wyeth Corp Metodos de separacion y deteccion de acetato de bacedoxifeno en composiciones farmaceuticas
JP5168604B2 (ja) * 2008-05-08 2013-03-21 味の素株式会社 タンパク質のリフォールディング方法
JP6081908B2 (ja) * 2010-03-31 2017-02-15 アーエムシルク ゲーエムベーハー 不溶性の標的タンパク質の分離

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0114506A1 (fr) * 1982-12-22 1984-08-01 Genentech, Inc. Méthodes de purification et réactivation des protéines hétérologues précipitées

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4443539A (en) * 1980-02-05 1984-04-17 The Upjohn Company Process for preparing bovine growth hormone
IL59690A (en) 1980-03-24 1983-11-30 Yeda Res & Dev Production of bovine growth hormone by microorganisms and modified microorganisms adapted to produce it
IE52755B1 (en) 1980-08-26 1988-02-17 Univ California Bovine pre-growth and growth hormone
EP0085036A1 (fr) 1982-01-18 1983-08-03 Monsanto Company Méthode pour une production améliorée de lait de bovins
CA1224432A (fr) 1982-08-17 1987-07-21 Gary N. Buell Sequences d'adn, molecules d'adn recombinant et procede de production a grande echelle de polypeptides ressemblant a l'hormone de croissance des bovins
US4512922A (en) * 1982-12-22 1985-04-23 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4518526A (en) * 1982-12-22 1985-05-21 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4620948A (en) * 1982-12-22 1986-11-04 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511503A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4599197A (en) * 1982-12-22 1986-07-08 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511502A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
BG49718A3 (bg) 1983-07-15 1992-01-15 Bio- Technology General Corp Метод за получаване на полипептид със супероксиддисмутазна активност
US5037806A (en) * 1985-02-22 1991-08-06 Monsanto Company Biologically active method using somatotropins
US4652630A (en) * 1985-02-22 1987-03-24 Monsanto Company Method of somatotropin naturation
US4612367A (en) * 1985-04-08 1986-09-16 Eli Lilly And Company Process for purifying growth hormone-like materials
DE3685424D1 (de) * 1985-06-26 1992-06-25 Upjohn Co Solubilisation und oxidation von somatotropin aus transformierten mikroorganismen.
US5023323A (en) * 1990-09-12 1991-06-11 Monsanto Company Method of somatotropin naturation
US5240834A (en) * 1991-01-22 1993-08-31 Albert Einstein College Of Medicine Of Yeshiva University Solubilization of protein after bacterial expression using sarkosyl

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0114506A1 (fr) * 1982-12-22 1984-08-01 Genentech, Inc. Méthodes de purification et réactivation des protéines hétérologues précipitées

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
B.D. DAVIS et al.: "Microbiology", 3rd edition, 1980, Harper & Row, New York (US), see page 1271, right-hand column, lines 9-13 *
CHEMICAL ABSTRACTS, Volume 66, No. 5, 30 January 1967, Columbus, Ohio (US) M. TEUBER: "Degradation of RNA from Escherichia Coli after Cytoplasmic Membrane Destruction by Toluene" see pages 1593-4, Abstract 16597y & Arch. Mikrobiol. 55(1), 31-45 (1966) *
CHEMICAL ABSTRACTS, Volume 81, No. 17, 28 October 1974, Columbus, Ohio (US) A.M. PLOMMET et al.: "Killing of Diverse Pathogenic Bacteria in Bovine Liquid Manure by Xylene", see page 138, Abstract 100544w, & Ann. Rech. Vet. 1974, 5(2), 213-21 *

Also Published As

Publication number Publication date
EP0229110B1 (fr) 1992-05-20
US6586575B1 (en) 2003-07-01
US6916914B1 (en) 2005-07-12
JPH06104070B2 (ja) 1994-12-21
DE3685424D1 (de) 1992-06-25
EP0263902B1 (fr) 1992-05-27
WO1987000204A2 (fr) 1987-01-15
JPS62503143A (ja) 1987-12-17
HK64897A (en) 1997-05-23
ES2032383T3 (es) 1993-02-16
ATE76435T1 (de) 1992-06-15
GR3004917T3 (fr) 1993-04-28
EP0263902A1 (fr) 1988-04-20
EP0229110A1 (fr) 1987-07-22

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