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WO1986006488A1 - Kit pour tests diagnostiques - Google Patents

Kit pour tests diagnostiques Download PDF

Info

Publication number
WO1986006488A1
WO1986006488A1 PCT/US1986/000709 US8600709W WO8606488A1 WO 1986006488 A1 WO1986006488 A1 WO 1986006488A1 US 8600709 W US8600709 W US 8600709W WO 8606488 A1 WO8606488 A1 WO 8606488A1
Authority
WO
WIPO (PCT)
Prior art keywords
housing
plastic
liquid
liquid reservoir
bubble
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1986/000709
Other languages
English (en)
Inventor
Burton Barnett
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HICHEM DIAGNOSTICS Inc dba BURAL TECHNOLOGIES
Original Assignee
HICHEM DIAGNOSTICS Inc dba BURAL TECHNOLOGIES
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HICHEM DIAGNOSTICS Inc dba BURAL TECHNOLOGIES filed Critical HICHEM DIAGNOSTICS Inc dba BURAL TECHNOLOGIES
Publication of WO1986006488A1 publication Critical patent/WO1986006488A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures

Definitions

  • the present invention is in the field of diagnostic kits and devices. More particularly, the present invention is directed to an apparatus or device which contains the necessary reagents for the performance of diagnostic, immunoassay, and like tests on a sample or specimen, and which facilitates the performance of such tests.
  • Positive results of diagnostic immunoassay and like -tests are usually indicated by visually perceptible changes, such as color changes, which occur in the treated sample or specimen in the final steps of the tests.
  • commonly used clinical diagnostic, immunoassay, and like tests usually test the blood, urine, or other body fluids or tissues, for the presence of certain chemicals, hormones, antigens, pathogens, or abnormal cells.
  • a mere "positive” or “negative” evaluation of the sample or specimen is desired with reference to the presence or absence of the chemical, hormone, antigen, or pathogen which is evaluated by the test.
  • a quantitative evaluation of the sample or specimen is made with regard to the level of chemical, hormone, antigen, or pathogen.
  • the quantitative evaluation is often made by spectrophotometric measurement of the color intensity, and/or of other physical property or properties of the sample or specimen.
  • test kit apparatus which includes a housing and a sample receiving area, or well, defined in the housing and adapted for receiving a sample or specimen. At least one, but preferably a plurality of rupturable containers, are mounted in the housing. Each container contains the predetermined amount of one of the specific reagents which are required for the performance of the particular diagnostic or immunoassay test for which the kit is designed.
  • a duct or channel connects each area below each container with the sample receiving area or well, where the sample or specimen is located.
  • a pointed protrusion, or other like member preferably integrally molded with the housing, is provided in operative association with each container, to enable a technician operator to rupture the container when addition of the specific reagent is required in the test.
  • the liquid or powder reagent content of each container flows through the respective duct or channel into the sample receiving area or well where the test reactions occur.
  • a filter paper or similar member supporting the antibody is disposed in the sample receiving area or well.
  • a porous plate is disposed below the antibody supporting member to permit the excess reagents and wash solutions to drain into a liquid absorbing material or into a liquid reservoir space disposed below.
  • the containers instead of being ruptured or sheared by an object, may contain a weakened wall section whereby they can be ruptured by application of pressure only.
  • Figure 1 is a perspective view of a first preferred embodiment of the test kit apparatus of the present invention
  • Figure 2 is a partial top view of the first preferred embodiment shown on Figure 1, the view showing a transparent protective cover member covering the top of the embodiment;
  • Figure 3 is a cross-sectional view taken on lines 3,3 of Figure 1;
  • Figure 4 is a partial top view of the first preferred embodiment shown on Figure 1, without the transparent protective cover member;
  • Figure 5 is a partial view of the first preferred embodiment, the view being taken on lines 5,5 of Figure 3;
  • Figure 6 is a cross-sectional view taken on lines 6,6 of Figure 4.
  • Figure 7 is a partial cross-sectional view of a second preferred embodiment, the view showing an intact container for a liquid reagent incorporated in the embodiment;
  • Figure 8 is a partial cross-sectional view of the second preferred embodiment, the view showing the container ruptured by a shearing device incorporated in the embodiment;
  • Figure 9 is a partial cross-sectional view of a third preferred embodiment, the view showing an intact container for a liquid reagent incorporated in the embodiment;
  • Figure 10 is a perspective view of a plastic or like sheet to which plastic bubble containers for liquid reagents are affixed in accordance with the present invention
  • Figure 11 is a partial perspective view of a fourth preferred embodiment
  • Figure 12 is a partial top view of the fourth preferred embodiment
  • Figure 13 is a perspective view of a fifth preferred embodiment
  • Figure 14 is a partial top view of a sixth preferred embodiment
  • Figure 15 is a cross-sectional view of a seventh preferred embodiment
  • Figure 16 is a partial top view of an eighth preferred embodiment
  • Figure 17 is a partial top view of a ninth preferred embodiment.
  • Figure 18 is a partial top view of a tenth preferred embodiment.
  • the present invention is generally and broadly directed to test kits or apparatus which can be adapted to a large variety of chemical, clinical diagnostic, immunoassay, and like tests.
  • the invention is not limited by the type of the test performed in the apparatus.
  • the specific examples of diagnostic or immunoassay tests which are made in the present description should, therefore, be considered exemplary, rather than limiting in nature.
  • the test apparatus or kit of the present invention includes a housing 22 wherein, in accordance with the present invention, all, or nearly all, reagents required for performing the test are contained in the specific amounts required in the test.
  • the housing 22 is preferably made of molded plastic and comprises a bottom plate 24, an upper plate 26, and an intermediary member 28, best shown on the cross-sectional views of Figures 3 and 6.
  • the foregoing component parts of the housing 22 are preferably attached to one another by sonic welding, gluing, or the like processes which readily adapt themselves to mass manufacturing.
  • a portion of the top surface of the upper plate 26 of the housing 22 preferably carries indicia 30 providing step-by-step directions for performing the specific test. It should be understood that the indicia 30, that is, the directions for performing the specific test, depend on the nature of the test. The indicia or directions 30 are only schematically shown on Figure 1.
  • the housing 22 defines a first sample receiving area 32 and a plurality of additional areas 34 for receiving a plurality of small containers 36 which contain the liquid reagents 38 required for the test. More specifically stated, and with reference to Figures 1-6 of the appended drawings, the sample receiving area 32 is a well, formed in the intermediate member 28 of the housing 22, and the additional areas 34 each comprise an additional, although preferably smaller, well or depression also formed in the intermediate member 28 in the housing 22.
  • Such an embodiment is, of course, preferably used in a test where only a single reagent is required.
  • the sample receiving area or well 32 is located below an opening 40 in the upper plate 26. Moreover, each of the additional wells or depressions 34 in the intermediate member 28 are located below respective openings 42 in the upper plate 26.
  • Each of the wells or depressions 34 are connected to the main, sample receiving well 32 by a duct or channel 44 so that liquid or powdery reagents can flow from the wells or depressions 34 to the main sample receiving well 32.
  • the ducts or channels 44 are integrally molded with the intermediate member 28 of the housing 22, and comprise only a small volume relative to the liquid capacity of either the small containers 36 or of the main sample receiving well 32.
  • a sharp protrusion or spike 46 is located substantially in the center of each well or depression 34.
  • the spikes 46 are integrally molded with the intermediate member 28 of the housing 22.
  • Each container 36 comprises a sealed plastic bubble.
  • Each bubble 36 contains the predetermined amount of one of the specific liquid reagents 38 required in the specific diagnostic, immunoassay, or like test for which the test kit is designed.
  • Each plastic bubble 36 is located substantially below the respective opening 42 in the upper plate 26 of the housing 22, and above the respective spike 46 of the corresponding well 34.
  • the plastic bubbles 36 are affixed to a substantially flat plastic sheet 48, which is shown in the cross-sectional views of the drawing Figures, and also on Figure 10.
  • the plastic bubbles 36 are preferably formed in a film of plastic 49, and the plastic sheet 48 serves as their closure.
  • the plastic sheet 48 has a cut off corner 50 which serves, together with a matching shoulder (not shown) in the housing 22, as a key during assembly of the test kit. This assures that the plastic sheet 48, bearing the plastic bubbles 36, can be properly mounted in the housing 22 in only one orientation.
  • Indicia 52 such as the arabic numerals 1, 2, 3, and 4, are located adjacent to each of the openings 42 below which the plastic bubble containers 36 are disposed. These indicia or numerals 52 identify the specific liquid reagent 38 contained therein.
  • the numerals 52 together with the directions 30 (and possibly in combination with other instructions regarding the specific test to be performed) , provide the information on the basis of which even a semi-skilled technician (not shown) is able to perform the specific chemical, diagnostic, or immunoassay test for which the particular test apparatus is specifically designed.
  • the sample 54 may be a liquid, such as a body fluid, blood, blood serum, or urine. In other than medical diagnostic tests, the sample 54 may be some other liquid, such as water, as, for example, in tests designed for drinking water, swimming pool water, or the like.
  • the sample or specimen 54 may also be solid or semi-solid material, such as a tissue sample which is required in many diagnostic or pathology laboratory tests.
  • the sample 54 may also be a soil sample for soil analysis.
  • the apparatus of the present invention also includes the solid phase bound antibody (not shown) . More specifically, and with reference to Figure 6 showing the first specific embodiment 20 in cross-section, a porous plate 56 is located below the main sample receiving well 32, and a liquid absorbing material 58 is located below the porous plate 56 in the housing 22 just above the bottom plate 24.
  • the porous plate 56 is of the type which permits slow drainage of liquid, and may be made of materials commonly used for this purpose in the laboratory equipment and related arts.
  • the liquid absorbing material 58 preferably comprises cellulose, the exact nature of the absorbant material 58, as well as of the porous plate 56, not being critical for the purposes of the present invention.
  • the total amount of the absorbant material or absorbant padding 58 is, however, such that it is capable of absorbing all, or virtually all, liquid reagent 38 which gradually drains through the porous plate 56.
  • a filter paper or filter membrane 60 is shown disposed above the porous plate 56.
  • the filter paper or membrane 60 comprises the bottom of the main sample receiving area or well 32.
  • the filter paper or membrane 60 contains the bound antibody which is required in the specific test.
  • the technician operator After the sample or specimen 54 is placed on the filter paper or membrane 60 in the main well 32, the technician operator (not shown) waits the requisite amount of time (if such time is necessary) to permit binding of the sought-after component (not shown) to occur to the solid supported antibody (not shown) .
  • the sequence of steps to be followed, including waiting periods between the successive steps are described in the directions contained in the indicia 30.
  • the technician operator presses, through the respective opening 42 in the housing 22, the specifically identified plastic bubble container 36 against the spike 46.
  • the plastic sheet 48 which forms the closure of the plastic bubble container 36, comprises the upper closure for the ducts 44. This is best shown on the cross-sectional view of Figure 6.
  • the progress of the test, as well as the final color or other visually perceptible change indicating the outcome of the test, is observed through the opening 40 located above the main sample receiving well 32.
  • Figures 1 and 2 show a protective plastic cover 62 disposed on the top surface of the upper plate 26 of the housing 22.
  • the protective plastic cover 62 is an optional component of the apparatus of the invention. It protects the plastic bubble containers 36 from accidental rupture, either by a sharp external object, or through inadvertently pressing against the spikes 46, during shipping and handling.
  • the protective cover 62 may carry the indicia 30.
  • the protective cover 62 is preferably transparent, and is preferably affixed to the housing 22 with a pressure sensitive adhesive (not shown) which permits its removal by peeling.
  • Figure 2 shows a separate portion 64 of the protective sheet 62, which can be replaced on the housing 22 after the sample or specimen 54 has been added.
  • each plastic bubble container 36 incorporates a relatively narrow section 68.
  • the narrow section 68 is disposed below a vertically movable member or plug 70 placed in a substantially vertically formed bore 71 in the housing 22.
  • the plug 70 is pressed downwardly to shear off the narrow section 68.
  • a circumferential groove or notch 72 in the plug 70 permits the liquid reagent to flow around the plug 70 through the respective duct 44, to the main sample receiving well 32.
  • Figure 9 shows a third preferred embodiment 74 of the test apparatus of the present invention.
  • the plastic bubble containers 36 include a weakened wall section 76 designed to rupture under internal pressure which is attained when the bubble container 36 is finger-pressed by a technician-operator (not shown) . After the weakened wall section 76 is ruptured, the liquid reagent 38 is released into the main sample receiving well 32, substantially as in the above-described embodiments.
  • Figures 11 and 12 show a fourth preferred embodiment 78 of the test apparatus of the present invention.
  • This embodiment is primarily designed for use in tests where it is desirable to remove the sample or specimen after the test has been completed, for example, for the purposes of conducting spectrophotometric or radiation counting assays.
  • the main sample receiving area or well 32 is located in a tray 80, which is inserted through an appropriate opening 82 into the housing 22.
  • the sample receiving well 32 may be divided into sections 84.
  • the specimen may be placed in one or two of the three sections, and a known control or controls may be placed in the remaining section or sections 84. In such cases, the test indicates its own reliability, in that, if the test functions properly, the section or sections containing the controls should exhibit the expected positive or negative results.
  • Figure 13 shows a fifth preferred embodiment 86 of the test apparatus of the present invention, wherein a liquid sample or specimen 54 may be placed into the sample receiving area or well 32 through an appropriate, substantially horizontally disposed aperture 88 in the housing 22.
  • Figure 14 shows a sixth preferred embodiment 90.
  • one or two ducts or channels 44 fluidly interconnect with one another, before they reach the main sample receiving well 32.
  • This embodiment is primarily useful in tests where two reagents 38 must be added substantially simultaneously, and should be well mixed with one another.
  • Figure 15 shows a seventh preferred embodiment 92.
  • This embodiment 92 differs principally from the above-described embodiments (primarily the first preferred embodiment 20 which was described in detail) in that an empty chamber 94, capable of acting as a liquid reservoir, is located above the bottom plate 24 of the housing 22.
  • the bottom plate 24 is flexible, and has an aperture 96 equipped with a one-way check valve 98 which allows air to exit from the chamber 94 when the flexible bottom plate 24 is compressed.
  • the seventh preferred embodiment 92 has no liquid absorbing padding or like material.
  • the chamber 94 in the housing 22 acts as a reservoir for used liquid reagents.
  • the above-described feature of the seventh preferred embodiment also permits relatively fast removal of the liquid reagents from the sample receiving well 32.
  • the eighth preferred embodiment 100 is a variation of the first preferred embodiment 20, and principally differs from the first preferred embodiment only in that a solid reagent 102 is affixed in the sample receiving well 32.
  • the solid reagent 102 is, of course, one of the reagents which is required for the performance of the specific diagnostic or like test for which the specific test apparatus is designed.
  • the solid reagent 102 may be an enzyme, or an antibody, and that, in many instances, enzymes and other protein comprising reagents are more stable in a solid (lyophilized or the like) state than in solution.
  • the solid reagent 102 may be affixed to the sample receiving well 32 as a tablet, as is shown on Figure 16, or may be otherwise deposited in the sample receiving well 32, as, for example, in the form of a concentrated solution which is thereafter permitted to dry.
  • the "active" solid reagent 102 such as an enzyme or antibody, is likely to be admixed with other inert or auxiliary solid materials, such as stabilizing agents or desiccants.
  • a solid reagent 102 is contained within a plastic bubble 36 which is disposed above one of the wells or depressions 34 formed in the intermediate member 28 of the housing 22.
  • the channel or duct 44 leads from yet another well 34 to the well containing the solid reagent 102. Therefore, when the liquid contents 38 of the plastic bubble 36 are released, the solid reagent 102 becomes exposed to the liquid reagent to be dissolved therein. The freshly created solution is then added to the main sample receiving well 32.
  • the embodiment shown on Figure 18 is similar to the embodiment shown on Figure 17, with the only substantial difference that liquid reagents are added from two plastic bubbles to the solid reagent 102 encased in a plastic bubble 36. It is noted in this regard that, in accordance with the varying requirements of specific tests in the embodiments shown on Figures 17 and 18, the ducts 44 and the respective plastic bubbles 36 may be formed in such a manner that the liquid reagent enters the plastic bubble which encases the solid reagent 102.
  • HCG human chorionic gonadotropin hormone
  • the filter paper membrane 60 contains, bound to its cellulose fibers, a monoclonal antibody which reacts with and binds to the HCG molecule.
  • the indicia or directions 30 describe the following steps, and the plastic bubble containers 36, sequentially numbered 1 through 4, contain the appropriate, below-described reagents or wash solutions 38.
  • a small, predetermined quantity of urine sample (of the human female subject) is placed on the filter paper 60 in the main sample receiving well 32.
  • the urine sample is allowed to completely drain through the filter paper 60 and porous plate 56 into the underlying absorbant material 58.
  • the HCG content of the urine (if there is any) binds to the monoclonal antibody, and is retained in the filter paper or membrane 60.
  • the plastic bubble 36 identified with the arabic numeral 1
  • This reagent in the herein-described test for HCG, contains an antibody chemically linked to an enzyme.
  • the reagent containing the antibody-linked enzyme is allowed to completely drain through the filter paper 60 and porous plate 56 into the absorbant material 58.
  • the antibody binds to the HCG already bound on the filter paper or membrane 60.
  • the enzyme, coupled to the antibody is capable of causing a color reaction in a subsequent step of the assay. It follows from the foregoing that, if there is no HCG in the urine sample, there is no binding of the antibody-linked enzyme to the filter paper during this step.
  • the plastic bubble 36 is ruptured through pressing against the underlying spike 46.
  • the reagent contained in this bubble 36 is a wash solution, such as saline.
  • the wash solution is allowed to drain completely through the filter 60 and porous plate 56.
  • the wash solution washes away any HCG which was not immobilized in the filter 60 by binding to the antibody contained therein. It also washes away any antibody-linked enzyme which was not immobilized by binding to the HCG molecules bound to the antibody immobilized on the ilter 60.
  • the plastic bubble 36 is ruptured to release a "color developer” reagent into the sample receiving well 32.
  • the "color developer” reagent is a mixture of chemicals which react and develop, or change, color under the catalytic activity of the enzyme bound in the filter 60.
  • the plastic bubble 36 is ruptured to release a wash solution, and to wash away the "color developer” from the filter 60, thereby stopping the color development process.
  • the intensity of color developed in the filter 60 is proportional to the amount of HCG hormone present in the urine sample. Lack of color indicates a negative, "not pregnant” result.
  • the reagents are separated from one another, and not mixed until immediately before the test. In some instances, this is particularly important, as, for example, in the well-known "Trinder methodology" for cholesterol determination.
  • This methodology free cholesterol is oxidized in the presence of cholesterol oxidase enzyme to cholesten-3-one with the production of hydrogen peroxide. Hydrogen peroxide reacts, in the presence of peroxidase enzyme, with 4-amino anti-pyrine and phenol to yield a spectrophotometrically measurable colored dye.
  • the mixture of 4-amino anti-pyrine and phenol is not stable in aqueous solutions. Therefore, in the prior art, the reagent kits for the Trinder methodology contained lyophilized mixtures of these two reagents, which had to be reconstituted shortly before use.
  • the aqueous solution of phenol is kept in a separate container from the aqueous 4-amino anti-pyrine, whereby the problem of low stability is eliminated, and there is no need for relatively expensive lyophilized reagents.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Appareil de test facilitant l'exécution de tests chimiques, cliniques, diagnostiques d'analyses immunologiques et analogues. L'appareil comprend un boîtier (22) et une zone ou puits (32) recevant un échantillon, défini dans le boîtier et destiné à recevoir un échantillon ou spécimen. Une pluralité de conteneurs cassables (36) sont montés dans des évidements (34) formés dans le boîtier. Chaque conteneur cassable contient la quantité nécessaire de réactif spécifique (38) nécessaire pour le test. Une conduite ou canal (44) relie par une circulation de fluide chaque évidement (34) avec la zone ou puits (32) recevant l'échantillon. Une pointe effilée ou un organe présentant un bord tranchant (46) est monté dans le boîtier et est associé à chaque conteneur cassable (36) pour permettre à un opérateur de casser les conteneurs (36) suivant la séquence requise par le test, afin de provoquer l'écoulement des réactifs liquides (38) vers la zone ou puits (32) recevant l'échantillon.
PCT/US1986/000709 1985-04-29 1986-04-10 Kit pour tests diagnostiques Ceased WO1986006488A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US72825585A 1985-04-29 1985-04-29
US728,255 1985-04-29
US76333285A 1985-08-06 1985-08-06
US763,332 1985-08-06

Publications (1)

Publication Number Publication Date
WO1986006488A1 true WO1986006488A1 (fr) 1986-11-06

Family

ID=27111661

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1986/000709 Ceased WO1986006488A1 (fr) 1985-04-29 1986-04-10 Kit pour tests diagnostiques

Country Status (2)

Country Link
EP (1) EP0221105A1 (fr)
WO (1) WO1986006488A1 (fr)

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US4978502A (en) * 1987-01-05 1990-12-18 Dole Associates, Inc. Immunoassay or diagnostic device and method of manufacture
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US6007999A (en) * 1991-07-31 1999-12-28 Idexx Laboratories, Inc. Reversible flow chromatographic binding assay
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JP2013518289A (ja) * 2010-01-29 2013-05-20 マイクロニクス， インコーポレイテッド サンプル−回答型のマイクロ流体カートリッジ
JP2014197016A (ja) * 2009-02-06 2014-10-16 ノースウエスタン ユニバーシティ 破裂可能な液体パッケージングとその使用
JP2016536590A (ja) * 2013-11-12 2016-11-24 ボディテックメド インコーポレイテッドＢｏｄｉｔｅｃｈｍｅｄ． Ｉｎｃ 一体化された反応及び検出手段を備えるマルチウェルキュベット
JPWO2015045134A1 (ja) * 2013-09-30 2017-03-02 株式会社日立製作所 試薬保持容器、送液装置、試薬吐出方法
WO2017104143A1 (fr) * 2015-12-18 2017-06-22 富士フイルム株式会社 Kit d'immunochromatographie
WO2017180047A1 (fr) * 2016-04-12 2017-10-19 Meje Ab Dispositif analytique à membrane pour fluides corporels
CN107462577A (zh) * 2017-08-25 2017-12-12 友好净控科技（浙江）有限公司 一种甲醛浓度检测设备、制备方法及检测方法
US10065186B2 (en) 2012-12-21 2018-09-04 Micronics, Inc. Fluidic circuits and related manufacturing methods
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CN110244034A (zh) * 2019-06-28 2019-09-17 京东方科技集团股份有限公司 检测装置、检测系统及检测方法
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US10518262B2 (en) 2012-12-21 2019-12-31 Perkinelmer Health Sciences, Inc. Low elasticity films for microfluidic use
US11428686B2 (en) * 2005-10-31 2022-08-30 Abbott Rapid Diagnostics International Unlimited Company Membrane assay method
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