[go: up one dir, main page]

WO1985004424A1 - Equipement de detection pour l'indication de la presence de gaz nervins et d'autres inhibiteurs de la cholinesterase - Google Patents

Equipement de detection pour l'indication de la presence de gaz nervins et d'autres inhibiteurs de la cholinesterase Download PDF

Info

Publication number
WO1985004424A1
WO1985004424A1 PCT/SE1985/000158 SE8500158W WO8504424A1 WO 1985004424 A1 WO1985004424 A1 WO 1985004424A1 SE 8500158 W SE8500158 W SE 8500158W WO 8504424 A1 WO8504424 A1 WO 8504424A1
Authority
WO
WIPO (PCT)
Prior art keywords
detector kit
enzyme
detector
substrate
kit according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/SE1985/000158
Other languages
English (en)
Inventor
Erik Dahlgren
Sture Bergek
Kaj MA^oRTENSSON
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of WO1985004424A1 publication Critical patent/WO1985004424A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C12Q1/46—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase involving cholinesterase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/525—Multi-layer analytical elements

Definitions

  • the present invention relates to a detector kit for the indication of nerve gases and other cholinesterase-inhibitors.
  • the present invention relates to a detector kit in one part, which is extremely simple to use and which can be produced at a Low cost and with simple means.
  • the invention is primarily meant to be used in a war situation where nerve gases might be used.
  • the person, who is going to use the equip ⁇ ment may in many cases be under a very strong physical and psychical pressure, the light conditions may be very poor and moreover, this person is wearing a protective mask and a protective suit which among other things involves the use of thick and clumsy gloves.
  • a protective mask and a protective suit which among other things involves the use of thick and clumsy gloves.
  • the equipment should be of an easily manageable size and be so devised that the indication can be performed with simple movements of one's hand. It is consequently disadvantageous with an equipment which consists of several small parts that are to be moved about during the indication. Moreover, the equipment should be so constructed that one can easily bring the enzyme and the substrate in contact with each other" during the indication.
  • the detector kit can in a folded form keep the substrate and the enzyme away from the ambient air without using a separate outer wrapping.
  • the enzyme must be wetted for the indication.
  • the enzyme surface has been wetted by means of especially provided water before the indication.
  • the detector kit is therefore near the enzyme provided with a container comprising water to wet the choLinesterase.
  • the container can be made to burst by means of the pressure from a finger.
  • the container may comprise a freezing point depressing means, for instance glycol, in order to make the detector kit usable in the cold. It is of very great practical importance that the individual user does not have to start looking for water when he wants to use the detector kit.
  • liquid container is protected from un ⁇ intentional puncturing during storage and transport. If the container has been punctured, this must be recognizable when the wrapping is broken. It is here of great importance if one can, not only visually, verify that the enzyme has been wetted, as this may be difficult during troublesome conditions.
  • the liquid container is so placed on the detector kit that it is broken and becomes loose ;after being punctured and thus clearly shows in this way that it has been punctured.
  • fig 1a shows a basic embodiment of the invention
  • fig 1b is a sectional view taken on the Line A-A of fig 1a
  • fig 2 shows an embodiment of the invention in a Longitudinal sectio ⁇ nal view with a liquid container close to the enzyme
  • ig 3a shows another embodiment of the invention in a longitudinal sectional view with a liquid container close to the enzyme
  • ig 3b shows the invention of fig 3a in a cross-sectional view through the enzyme section
  • ig 4 shows an embodiment of the invention with a three-section detec ⁇ tor kit, which can be folded twice and is provided with a liquid container
  • fig 5a shows an embodiment of the invention which is airtight per se during storage
  • fig 5b is a sectional view taken on the line B-B of fig 5a with the detector kit folded and provided with a desiccant and a sepa ⁇ rating foil
  • fig 6a shows still
  • choLinesterase 2 On a support 1 there are placed the enzyme choLinesterase 2 and also a substrate 3 for the enzyme.
  • the choLinesterase can suitably be immobi ⁇ lized and stabilized on paper.
  • the enzyme papers prepared in this way are dried, first in ambient air and then in an exsiccator under vacuum.
  • the substrate which has proved to have the best properties for an en ⁇ zymatic colour reaction, is 2,6-dichloro indophenyl acetate (DCIPA).
  • DCIPA 2,6-dichloro indophenyl acetate
  • the working up has been modified in as much, as DCIPA has been precipitated in water and then recrystallized in n-hexane:diethyl ether.
  • the substrate paper is produced by dissolving DCIPA in a suitable soL- vent, e.g. acetone, carbon tetrachloride, chloroform, dichloromethane or 1,2-dichloroethane, and then applying the solution in drops onto the paper. So much of the.solution is used that the whole paper is evenly.
  • a suitable soL- vent e.g. acetone, carbon tetrachloride, chloroform, dichloromethane or 1,2-dichloroethane
  • the surface concentration i.e. the amount of DCIPA per cm , is varied by changing the concentration of DCIPA in the solution.
  • the surface concentration is not critical as long as a sufficiently Large amount of the substrate is available in order to get a clear change in the enzymatic colour reaction. Be ⁇ cause of the losses that one must take into account during storage, in the form of decomposition and possibly sublimation of the substrate,
  • the Latter test has been accomplished by placing paper, impregnated with DCIPA, between two pieces of the plastic in question. The whole package has then been stored in heat at + 60 C together with a de- siccant. After 46 days the sample was taken out and examined with re ⁇ gard to whether DCIPA had diffused into the plastic or not and with regard to how well the DCIPA-papers functioned during indication.
  • plastic materials are considered usable for the pro- duction of detector kits using enzyme: Plexiglas,. polystyrene and polycarbonate.
  • a liquid container 4 can be placed close to the enzyme.
  • the container is provided with a pin 5, which perforates the support 1 when a finger is pressed there- on ; so that water will pass to the enzyme. It is also possible with other ways of arranging a Liquid container close to the enzyme.
  • figs 3a and 3b is illustrated how the water container 4 can be placed on the support 1 by means of a casing 10 pressed on to the support.
  • the casing is hard and brittle and is cracked on use, where ⁇ after it loosens from the support 1 thus giving a clear indication of the fact that the enzyme has been wetted. (Important for instance if wetting should occur unintentionally during storage.)
  • the sharp cracking sound and the detachment from the support give clear signals during correct handling that the enzyme has become satisfac ⁇ tory wetted.
  • fig 4 is illustrated a detector kit in three sections with the en ⁇ zyme 2 on the middle section.
  • a liquid container 4 is placed on a second section of the detector kit.
  • the enzyme 2 which can be provided with pins 5
  • the container 4 can be made to break so that water will wet the enzyme.
  • the substrate 3 is placed on the third section of the detector kit. After the wetting of the enzyme the second section is unfolded, and the third section is folded over the enzyme so that the enzyme and the substrate are brought into contact with each other.
  • figs 5a and 5b is illustrated an embodiment of the invention with a sealing device 6 around the substrate and the enzyme.
  • the sealing device consists of raised edges arranged on the support and extending aroung the enzyme and the substrate and of such dimensions that, when the detector kit is folded, one of the raised edges will come into a firm engagement with the other raised edge.
  • the two edges can have such a cross-section that they will snap into each other. In many cases, however, a sufficient sealing is obtained already with straight edges.
  • the raised edges are circular but other forms can of course be used.
  • figs 6a and 6b is illustrated another embodiment, which also is airtight per se during storage.
  • the edges 7 of the support for the enzyme and the substrate are here welded together or sealingly faste ⁇ ned to each other in another way, so that a folded detector kit is being formed comprising an airtight space in which the substrate and the enzyme are located. In use, the detector kit is torn apart, and then the procedure is continued as described above.
  • the enzyme and the substrate are kept apart during storage by means of. a foil 8 over the substrate.
  • the foil can be torn away after the detector kit has been opened or be so fastened over the' substrate that it is automatically torn away when the detector kit.is opened.
  • a thin aluminium foil has been used with a good result.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

Pendant l'indication, une surface de cholinestérase et une surface de substrat sont mises en contact l'une avec l'autre. L'équipement d'indication de l'art antérieur était formé de plusieurs parties séparées et était donc difficile à manipuler lorsque l'on était habillé de vêtements de protection encombrants. L'équipement de détection de la présente invention n'est formé que d'une partie qui est repliable, si bien que les deux surfaces peuvent être mises en contact. Cet équipement de détection est donc d'une manipulation aisée.
PCT/SE1985/000158 1984-04-02 1985-04-02 Equipement de detection pour l'indication de la presence de gaz nervins et d'autres inhibiteurs de la cholinesterase Ceased WO1985004424A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE8401798-7 1984-04-02
SE8401798A SE8401798L (sv) 1984-04-02 1984-04-02 Indikeringsbricka for nervgas och andra kolinesterashemmare

Publications (1)

Publication Number Publication Date
WO1985004424A1 true WO1985004424A1 (fr) 1985-10-10

Family

ID=20355393

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SE1985/000158 Ceased WO1985004424A1 (fr) 1984-04-02 1985-04-02 Equipement de detection pour l'indication de la presence de gaz nervins et d'autres inhibiteurs de la cholinesterase

Country Status (5)

Country Link
EP (1) EP0177600A1 (fr)
JP (1) JPS61501679A (fr)
AU (1) AU4230785A (fr)
SE (1) SE8401798L (fr)
WO (1) WO1985004424A1 (fr)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3914801A1 (de) * 1989-05-05 1990-11-08 Draegerwerk Ag Pruefroehrchen zum nachweis von schadstoffen
DE3929541A1 (de) * 1989-09-06 1991-03-14 Draegerwerk Ag Nachweisgeraet zur enzymatischen bestimmung von gasproben
WO1993001830A1 (fr) * 1991-07-22 1993-02-04 Bio-Technology General Corp. Expression de l'acetylcholinesterase humaine de recombinaison active sur le plan enzymatique et ses utilisations
WO2000065081A3 (fr) * 1999-04-26 2001-04-12 Us Army Med Res Mat Command Enzymes immobilisees servant de biodetecteurs de toxines chimiques
RU2165458C1 (ru) * 1999-10-07 2001-04-20 Гайнуллина Эра Тазетдиновна Способ определения необратимых ингибиторов холинэстеразы в воде и водных экстрактах
RU2182929C2 (ru) * 2000-07-21 2002-05-27 Институт прикладной механики РАН Комплект для определения ингибиторов холинэстеразы
RU2286559C1 (ru) * 2005-06-14 2006-10-27 Общество с ограниченной ответственностью "ЛАБОРАТОРИЯ БИОХИМИЧЕСКИХ МЕТОДОВ" Индикаторная трубка для контроля вредных веществ и способ экспресс-анализа воздуха
WO2012110693A1 (fr) * 2011-02-18 2012-08-23 Wallac Oy Procédé d'amélioration de la qualité et de la fonctionnalité de papier filtre utilisé pour recueillir des échantillons biologiques
EP3196315A1 (fr) 2016-01-19 2017-07-26 ORITEST spol. s r.o. Pastilles sphériques, processus de fabrication associé, utilisation et tube de détection comprenant de telles pastilles

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2465427A1 (fr) * 2004-04-28 2005-10-28 Imi International Medical Innovations Inc. Dosage direct du cholesterol dans des echantillons de peau preleves au moyen d'un ruban adhesif

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3689224A (en) * 1966-04-13 1972-09-05 Westinghouse Electric Corp Chemical contaminant detection sampler
US3809617A (en) * 1972-11-15 1974-05-07 American Cyanamid Co Device for detecting anticholinesterase materials
US4120754A (en) * 1975-10-04 1978-10-17 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Ten Behoeve Van De Rijksverdediging Detection of alkylating compounds
US4324858A (en) * 1980-06-16 1982-04-13 Midwest Research Institute Stabilization of cholinesterase, detector kit using stabilized cholinesterase, and methods of making and using the same

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3689224A (en) * 1966-04-13 1972-09-05 Westinghouse Electric Corp Chemical contaminant detection sampler
US3809617A (en) * 1972-11-15 1974-05-07 American Cyanamid Co Device for detecting anticholinesterase materials
US4120754A (en) * 1975-10-04 1978-10-17 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Ten Behoeve Van De Rijksverdediging Detection of alkylating compounds
US4324858A (en) * 1980-06-16 1982-04-13 Midwest Research Institute Stabilization of cholinesterase, detector kit using stabilized cholinesterase, and methods of making and using the same

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3914801A1 (de) * 1989-05-05 1990-11-08 Draegerwerk Ag Pruefroehrchen zum nachweis von schadstoffen
DE3929541A1 (de) * 1989-09-06 1991-03-14 Draegerwerk Ag Nachweisgeraet zur enzymatischen bestimmung von gasproben
WO1993001830A1 (fr) * 1991-07-22 1993-02-04 Bio-Technology General Corp. Expression de l'acetylcholinesterase humaine de recombinaison active sur le plan enzymatique et ses utilisations
WO2000065081A3 (fr) * 1999-04-26 2001-04-12 Us Army Med Res Mat Command Enzymes immobilisees servant de biodetecteurs de toxines chimiques
RU2165458C1 (ru) * 1999-10-07 2001-04-20 Гайнуллина Эра Тазетдиновна Способ определения необратимых ингибиторов холинэстеразы в воде и водных экстрактах
RU2182929C2 (ru) * 2000-07-21 2002-05-27 Институт прикладной механики РАН Комплект для определения ингибиторов холинэстеразы
RU2286559C1 (ru) * 2005-06-14 2006-10-27 Общество с ограниченной ответственностью "ЛАБОРАТОРИЯ БИОХИМИЧЕСКИХ МЕТОДОВ" Индикаторная трубка для контроля вредных веществ и способ экспресс-анализа воздуха
WO2012110693A1 (fr) * 2011-02-18 2012-08-23 Wallac Oy Procédé d'amélioration de la qualité et de la fonctionnalité de papier filtre utilisé pour recueillir des échantillons biologiques
US9677810B2 (en) 2011-02-18 2017-06-13 Wallac Oy Method for improving quality and functionality of filter paper suitable for collecting biological samples
EP3196315A1 (fr) 2016-01-19 2017-07-26 ORITEST spol. s r.o. Pastilles sphériques, processus de fabrication associé, utilisation et tube de détection comprenant de telles pastilles

Also Published As

Publication number Publication date
SE8401798D0 (sv) 1984-04-02
AU4230785A (en) 1985-11-01
EP0177600A1 (fr) 1986-04-16
SE8401798L (sv) 1985-10-03
JPS61501679A (ja) 1986-08-14

Similar Documents

Publication Publication Date Title
WO1985004424A1 (fr) Equipement de detection pour l'indication de la presence de gaz nervins et d'autres inhibiteurs de la cholinesterase
US4987085A (en) Blood filtering metering device
AU561000B2 (en) Microporous adhesive tape
NO781722L (no) Ventilert filtersigarett.
US4073713A (en) Membrane for enzyme electrodes
US4511533A (en) Test kit for performing a medical test
WO2000073794A3 (fr) Procede de separation des cellules par utilisation de rosettes immunitaires
US4631174A (en) Multilayer chemical analysis member having an outer waterproof layer
WO2002009591A3 (fr) Procede et dispositif de fermeture de ponction vasculaire a l'aide d'une matiere hemostatique
WO2002005865A3 (fr) Dispositif monte sur gaine pour mise en place d'un bouchon arteriel
JPH0251605B2 (fr)
CA2375453A1 (fr) Papier reactif en ecoulement lateral bidirectionnel et procede
JPH01163666A (ja) 皮膚透過物検出システム
FR2557300A1 (fr) Tampon experimental et procede pour detecter le sang occulte
CA2368103A1 (fr) Appareil et procede de prelevement et de manipulation d'echantillons tissulaires en vue d'une analyse de biopsie
US4582685A (en) Test kit for performing a medical test
NO843092L (no) Sammensatt, tett membran, fremgangsmaate ved fremstilling av denne og fremgangsmaate ved separasjon av fluider under anvendelse av membranen
US3435944A (en) Packing of hypodermic needle assembly
AU5426286A (en) Proteins immobilised on polyamides or cellulose hydrate and the use thereof for the preparation of biocatalysts, test strips or chromatography materials
Roberts et al. The effects of exposure to sub-lethal concentrations of cadmium on enzyme activities and accumulation of the metal in tissues and organs of rainbow and brown trout (Salmo gairdneri, Richardson and Salmo trutta fario L.)
WO2018209321A2 (fr) Appareil de détection adaptable
CH646587A5 (fr) Filtre a cigarette.
US2987174A (en) Test sample container
EP0304163A3 (fr) Elément analytique contenant de la peroxydase stabilisé
JP3172183B2 (ja) 支持媒体

Legal Events

Date Code Title Description
AK Designated states

Designated state(s): AU JP US

AL Designated countries for regional patents

Designated state(s): AT BE CH DE FR GB IT LU NL SE

WWE Wipo information: entry into national phase

Ref document number: 1985902181

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1985902181

Country of ref document: EP

WWR Wipo information: refused in national office

Ref document number: 1985902181

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1985902181

Country of ref document: EP