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UY25037A1 - Un proceso para producir material vegetal modificado geneticamente que comprende una o mas secuencias de adn de interes establemente incorporadas - Google Patents

Un proceso para producir material vegetal modificado geneticamente que comprende una o mas secuencias de adn de interes establemente incorporadas

Info

Publication number
UY25037A1
UY25037A1 UY25037A UY25037A UY25037A1 UY 25037 A1 UY25037 A1 UY 25037A1 UY 25037 A UY25037 A UY 25037A UY 25037 A UY25037 A UY 25037A UY 25037 A1 UY25037 A1 UY 25037A1
Authority
UY
Uruguay
Prior art keywords
plant material
plant
interest
genetically modified
dna
Prior art date
Application number
UY25037A
Other languages
English (en)
Inventor
Alyn Edwards Glyn
Original Assignee
Shell Int Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shell Int Research filed Critical Shell Int Research
Publication of UY25037A1 publication Critical patent/UY25037A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La invención se refiere a un proceso para la producción de material vegetal genéticamente modificado que comprende una o más secuencias de ADN de interés establemente incorporadas, comprendiendo tal proceso someter material vegetal que consiste de, o comprende, brotes, extremos o ápices de brotes, brotes enraizados o plántulas a una transferencia de la(s) secuencia(s) de ADN mediante Agrobacterium, siendo dicha transferencia de ADN mediante Agrobacterium llevada a cabo en un medio de cultivo líquido oxigenado, por ejemplo aireado, en el cual el material vegetal es cultivado, estando el material vegetal sumergido en el medio líquido. El medio generalmente es agitado o movido de otro modo. El material vegetal preferentemente es cultivado activamente en el medio de cultivo sumergido. El medio líquido está mantenido y con un pH determinado de modo tal que el crecimiento vegetal procede normalmente, pero el crecimiento del bacterium está restringido y a su vez que estimule la transferencia del ADN de interés desde el bacterium al material vegetal. Debe comprender suficiente oxígeno para soportar el metabolismo del material vegetal. Generalmente es necesario proveer oxígeno, usualmente en la forma de aire, y/o iluminar el sistema de modo que se produce oxígeno por fotosíntesis. También debe proveerse una fuente de carbono apropiada, por ejemplo, dióxido de carbono o una fuente de carbono orgánico. El material vegetal es obtenido a partir de un cultivar, clon o siembra. Puede ser una planta anual, bianual o perenne; planta mono o dicotiledónea; planta herbácea; planta leñosa, etc. La formación de brotes es inducida en el material vegetal genéticamente modificado en presencia de un agente capaz de inducir la formación de brotes, pudiendo ser este agente inductor una citoquina, 6-bencilaminopurina ó N-(2-cloro-4-piridil)-N'-fenilurea.
UY25037A 1997-06-13 1998-06-10 Un proceso para producir material vegetal modificado geneticamente que comprende una o mas secuencias de adn de interes establemente incorporadas UY25037A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP97304163 1997-06-13

Publications (1)

Publication Number Publication Date
UY25037A1 true UY25037A1 (es) 1998-12-11

Family

ID=8229377

Family Applications (1)

Application Number Title Priority Date Filing Date
UY25037A UY25037A1 (es) 1997-06-13 1998-06-10 Un proceso para producir material vegetal modificado geneticamente que comprende una o mas secuencias de adn de interes establemente incorporadas

Country Status (6)

Country Link
AR (1) AR015873A1 (es)
AU (1) AU8539698A (es)
IL (1) IL133168A0 (es)
UY (1) UY25037A1 (es)
WO (1) WO1998056932A1 (es)
ZA (1) ZA985078B (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6586661B1 (en) 1997-06-12 2003-07-01 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid
AR023576A1 (es) * 1999-04-19 2002-09-04 Biogemma Fr Metodo para la transformacion de una planta objetivo, uso de agrobacterium para dicho metodo y metodo para producir un tejido de planta transformada.
JP4099898B2 (ja) 1999-05-07 2008-06-11 王子製紙株式会社 ユーカリ属植物の成木を形質転換する方法
IT1317038B1 (it) * 2000-06-05 2003-05-26 Vitroplant Vivai Di Zuccherell Metodo per la rigenerazione di piante e suoi usi per lamoltiplicazione e/o la trasformazione di piante.
CN1638655A (zh) 2001-06-08 2005-07-13 韦克多烟草有限公司 修饰烟草中烟碱和亚硝胺的水平
EP1560484B1 (en) * 2002-03-20 2011-05-11 J.R. Simplot Company Refined plant transformation
CN116103337B (zh) * 2023-04-12 2023-07-11 中国热带农业科学院三亚研究院 一种农杆菌介导的西番莲遗传转化方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2211204B (en) * 1987-10-20 1992-05-20 Oji Paper Co Process for production of plant transformant
JP3565284B2 (ja) * 1994-09-22 2004-09-15 王子製紙株式会社 形質転換されたユーカリ属植物の作出方法
GB2298205A (en) * 1995-02-17 1996-08-28 Shell Int Research Genetic transformation of eucalyptus

Also Published As

Publication number Publication date
AU8539698A (en) 1998-12-30
WO1998056932A1 (en) 1998-12-17
IL133168A0 (en) 2001-03-19
ZA985078B (en) 1999-01-12
AR015873A1 (es) 2001-05-30

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Legal Events

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Effective date: 20130108