USPP36761P3 - Cannabis plant named ‘PTC8506V’ - Google Patents

Abstract

A new and distinct variety of Cannabis sativa designated ‘PTC8506V’ as herein described and illustrated, characterized by elevated levels of THCV.

Description

Genus/species: Cannabis sativa.

Variety denomination: ‘PTC8506V’.

BACKGROUND

Cannabis plants contain over a hundred known cannabinoids, which bind to endogenous endocannabinoid receptors. Varinolic cannabinoids, as known as varins, are a type of cannabinoid compounds having three carbon atoms in their alkyl side chain instead of the five carbon atom alkyl side chains more commonly associated with cannabinoids. Two such varins are tetrahydrocannabivarin (THCV) and cannabidivarin (CBDV), which are homologues of tetrahydrocannabinol (THC) and cannabidiol (CBD), respectively. Each varin has a unique pharmacological profile and distinct molecular targets.

THCV and CBDV have potential benefits across a broad set of applications. Cannabis plants or extracts with high THCV levels, for example, can be used as an agent for anticonvulsant activity, obesity-associated glucose intolerance, appetite suppression, anxiety management for PTSD, diabetic neuropathy, and major neuropathic and pain related pathologies. Another THCV application is that of an appetite suppressing compound. CBDV has been shown to have anti-epileptic and anticonvulsant activity.

Research and development as well as the sale of varin products has been limited due to low commonly occurring levels of varins in Cannabis flower. The ability to produce Cannabis with high varin levels will create a platform for a new Cannabinoid category with differentiated, high margin products in both medical and recreational markets. The present application solves these problems by providing a novel Cannabis plant having high THCV, which is referred to by the variety name ‘PTC8506V’.

SUMMARY

The new variety of Cannabis sativa was created by sourcing Cannabis plants that produce THCV and THC. Crosses were conducted to elevate further THCV, THC and/or other varins. All breeding, cultivation, and chemotype analyses were conducted in Hillsboro, Oregon. ‘PTC8506V’ resulted from the cross of a high THC pollen receiver plant (‘21GAQ-1271’, unpatented) and a high THCV pollen donor plant (‘21VLP5-1-102’, unpatented). ‘21GAQ-1271’ is a clonal variety with a THC content of 22.2%, and a THCV content below the limit of detection (LOD). ‘21GAQ-1271’ has greenish-purple flowers with heavy trichome coverage, moderate density, and strong aroma. ‘21GAQ-1271’ has an upright plant growth habit with moderately long internodes and matures in 8-9 weeks. ‘21VLP5-1-102’ had a THC content of 1.2%, and THCV content of 5.66%. ‘21VLP5-1-102’ flowers are greenish-purple with low trichome coverage, very low density, and moderately weak aroma. ‘21VLP5-1-102’ has a moderately wide plant growth habit with very long internodes and matures in 10-12 weeks. A clone of ‘21VLP5-1-102’ was crossed onto a clone of ‘21GAQ-1271’ in 2021. 32 of the resulting F1 seed were sown. ‘PTC8506V’ was discovered and selected as a single plant from this F1 seed and was subsequently propagated clonally in Hillsboro, OR. ‘PTC8506V’ was asexually reproduced by apical and sub-apical semi-herbaceous stem cuttings in Hillsboro, Oregon for eight cycles. Asexual reproduction has demonstrated that ‘PTC8506V’ reproduces true to type with all of the characteristics, as herein described, and firmly fixed and retained through successive generations of asexual propagation.

The new variety is named ‘PTC8506V’ and is characterized as having elevated levels of THCV. In comparison to its parents, ‘PTC8506V’ has higher THCV (relative to either parent), and higher THC relative to the pollen donor plant. ‘PTC8506V’ can additionally be distinguished from either parent at least based on the characteristics provided in Table 1.

TABLE 1
Comparison to Parent Varieties.

	Trichome	Aroma	Aroma	Growth	Inter-	Maturity
Variety	Coverage	Strength	Type	Habit	node	Time
‘21GAQ-	Heavy	Strong	Sweet	Upright	Moder-	8-9
1271’			cookies,		ately	weeks
			earth,		long
			garlic
‘21VLP5-1-	Low	Moder-	Grass,	Moder-	Long	10-12
102’		ately	earth,	ately		weeks
		weak	citrus	wide
‘PTC8506V’	Moderate	Moder-	Pine,	Upright	Moder-	8-10
		ately	earth,		ately	weeks
		strong	fuel,		long
			and a
			slight
			fruiti-
			ness

BRIEF DESCRIPTION OF THE PHOTOGRAPH

FIG. 1 is a photograph of a flowering plant of ‘PTC8506V’ that is approximately 83 days old, growing in Hillsboro, Oregon.

DETAILED BOTANICAL DESCRIPTION

The following is a detailed description of the new Cannabis variety ‘PTC8506V’. Unless otherwise indicated, all color designations provided herein are hex color codes.

All breeding, cultivation, and chemotype analyses were conducted in Hillsboro, Oregon.

Cannabis plants having elevated THCV or THC levels were sourced and obtained, and crosses were conducted to produce new varieties to further elevate THCV and THC levels. The plants were crossed to produce new varieties having elevated THCV and THC levels. Plant variety ‘PTC8506V’ was selected and asexually reproduced.

Table 2 describes the general characteristics of ‘PTC8506V’ at 55 days after lighting was changed to 12 hours on/12 hours off.

	TABLE 2
	Plant life forms	An herbaceous plant (herb)
	Plant growth habit	An upright, tap-rooted annual
		plant, forming fibrous
		roots when
		asexually propagated
	Plant origin	A controlled cross between
		pollen acceptor
		(‘21GAQ-1271’) and pollen donor
		plant (‘21VLP5-1-102).
	Plant propagation	Asexually propagated by apical
		and sub-apical semi-
		herbaceous stem cuttings
	Propagation Ease	Easy
	Plant shape	Simple erect
	Height	117 cm to 121 cm
	Width	87 cm to 101 cm
	Time to harvest	10-12 weeks
	Resistance to pests or diseases	Unknown
	GMO?	No

Table 3 describes the leaf/foliage of ‘PCT8506V’. Color codes are hex color codes.

TABLE 3
Leaf Arrangement	Alternate
Leaflet Type	Palmately compound
Leaflet Structure	Serrated margins, lightly acicular
	to lanceolate leaflets,
	tapering to an acuminate apex
Leaflet Margins	Jaggedly serrate with each tooth
	apex angled toward
	leaflet apex
Leaf hairs	Present on both upper and
	lower surfaces
Leaf length with petiole at	18.5 cm to 23 cm
maturity
Petiole length at maturity	6 cm to 9.5 cm
Petiole diameter at maturity	1.9 mm to 2.5 mm
Petiole color	#6B4247
Anthocyanin color and intensity	#F9CCC5. Medium intensity
in Petioles	in vegetative stage;
	stronger in flowering stage
Stipule length at maturity	6.2 mm to 6.8 mm
Stipule shape	Linear with slight curvaceous
	growth
Stipule color	#3C4D03
No. of leaflets	3 to 7
Middle largest (longest) leaflet	12 cm to 17.5 cm
length
Middle largest (longest) leaflet	2.5 cm to 3.1 cm
width
Middle largest (longest) leaflet	3.8 to 4.8
length/width ratio
No. teeth of middle leaflet	28 to 34
Leaf (upper side-adaxial) color	#35530A
Leaf (lower side-abaxial) color	#35530A
Leaf glossiness	Matte
Vein/midrib shape	Obliquely continuous
	throughout leaflet
Vein/midrib color	#E6DAA6
Aroma	Earthy notes of pine, fuel, and
	a slight fruitiness

Table 4 describes the stem of ‘PTC8506V’.

	TABLE 4
	Stem shape	Hollow, ribbed, texture
	Stem length	117 cm to 121 cm
	Stem strength	Strong and upright
	Stem diameter at base	13.6 mm to 14.5 mm
	Stem color	#373E02
	Depth of main stem ribs/grooves	Shallow
	Internode length	3 cm to 9.5 cm

Table 5 describes the inflorescence of ‘PTC8506V’.

TABLE 5
Inflorescence type	Elongated compound cymes
Flowering (blooming) habit	Natural flowering season is
	Autumn in Hillsboro, Oregon.
Proportion of female plants	100% pistillate
Inflorescence position	Terminal and axillary
Number of inflorescences	100 to 150
produced per plant
Flower arrangement	Cymose
Length of inflorescences	3 cm to 3.8 cm
Numbers of flowers per plant	Hundreds to thousands flowers per
	plant, multitudinous, congested
	with high concentrations on
	bushier wide inflorescence
Flower shape	Urceolate
Flower (individual pistillate)	5.1 mm to 5.3 mm
length
Flower (individual pistillate)	2 mm
depth
Flower (compound cyme)	5.5 cm to 8 cm
diameter
Corolla	No defined corolla
Corolla color	N/A
Bract shape	Urceolate
Bract color	#4B5D16
Typical bract numbers	3 to 200 bracts per inflorescence.
Bract length	3 mm to 5mm
Bract width	2.3 mm to 2.7 mm
Bract apex and base description	Bracts have a rounded base and
	caudate apex.
Bract Texture	Bract texture is smooth and
	covered with sticky trichomes.
Bracteole number, shape, length,	Bracteoles are not present
width, apex, margin, base
descriptors, texture, and color
Calyx shape	No defined calyx
Calyx color	N/A
Pistil number per flower	One
Pistil color	Pistil consists of the stigma, style,
	and ovary and cannot be
	given a single color.
Stigma shape	Acute
Number of stigmas per flower	Two
Stigma length	4.6 mm to 6.5 mm
Stigma color	#373E02
Trichome shape	Capitate-stalked glandular
Trichome color	#F1E7E2
Terminal inflorescence shape	Oblong
Terminal inflorescence color	#B7E1A1
Terminal inflorescence length	12.5 cm to 14 cm
Terminal inflorescence width	5 cm to 6.5 cm
Pedicel	Absent
Peduncle length	6.8 mm
Peduncle diameter	4.1 mm
Peduncle texture	Lightly ribbed
Peduncle color	#9BB53C
Staminate shape	No staminate flowers produced
	naturally; however, male
	flower (staminate) can be
	induced with stress or ethylene-
	blocking compounds
Sepal color	N/A
Pollen description	N/A
Petal description	Apetalous

Table 6 describes other characteristics of ‘PTC8506V’

	TABLE 6
	Time period and condition	8 to 10 weeks
	of flowering/blooming
	Proportion of hermaphrodite	Lower (<5%) in clones
	plants
	Hardiness of plant	Hardy to tolerate of warm
		and dry conditions
		without showing stress.
		Not tolerant to soil
		oversaturated with water
		for long periods

Chemical analyses were conducted using High Performance Liquid Chromatography to detect the cannabinoids described herein. Table 7 describes a chemotype analysis of ‘PTC8506V’, which was chemotyped by taking a sample from the main cola at day 56 after initiation of the 12:12 light cycle, which initiates flowering. Trichomes were 70% cloudy and 5% amber, indicative of full maturity at sampling.

	TABLE 7
	Cannabinoid and other chemicals	% wt

	Total THC	4.613
	Total THCV	11.067
	Total CBG	0.047
	Total CBD	<LOD
	Total CBC	<LOD
	Total CBGV	<LOD
	Total CBDV	<LOD
	alpha-Pinene	0.057
	Camphene	0.011
	beta-Myrcene	1.557
	beta-Pinene	0.098
	delta-3-Carene	<LOQ
	alpha-terpinene	<LOQ
	D-Limonene	0.211
	beta-Ocimene	0.275
	p-Cymene	<LOQ
	Eucalyptol	<LOQ
	gamma-Terpinene	<LOQ
	Terpinolene	<LOQ
	Linalool	0.003
	Isopulegol	<LOQ
	Geraniol	<LOQ
	beta-Caryophyllene	0.05
	alpha-Humulene	0.0227
	Nerolidol 1	<LOQ
	Nerolidol 2	0.011
	Guaiol	<LOQ
	Caryophyllene Oxide	0.011
	alpha-Bisabolol	0.026
	Total Terpenes	2.315

Claims (1)

What is claimed is:

1. A new and distinct variety of Cannabis sativa designated ‘PTC8506V’ as herein described and illustrated.

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