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USPP27939P3 - Stevia plant named ‘12-05-149’ - Google Patents

Stevia plant named ‘12-05-149’ Download PDF

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Publication number
USPP27939P3
USPP27939P3 US14/544,193 US201414544193V USPP27939P3 US PP27939 P3 USPP27939 P3 US PP27939P3 US 201414544193 V US201414544193 V US 201414544193V US PP27939 P3 USPP27939 P3 US PP27939P3
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Prior art keywords
rebaudioside
stevia
concentration
steviol glycosides
total steviol
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US14/544,193
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US20160165778P1 (en
Inventor
Ryan M. Warner
Randolph Beaudry
James Hancock
Veronica A. Vallejo
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Michigan State University MSU
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PureCircle Sdn Bhd
Michigan State University MSU
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Assigned to BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY, PURECIRCLE SDN BHD reassignment BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY
Publication of US20160165778P1 publication Critical patent/US20160165778P1/en
Assigned to BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY reassignment BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: VALLEJO, VERONICA
Assigned to BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY reassignment BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: PURECIRCLE SDN BHD
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/14—Asteraceae or Compositae, e.g. safflower, sunflower, artichoke or lettuce
    • A01H6/1488—Stevia

Definitions

  • Genus and species Stevia rebaudiana.
  • This invention relates to a new and distinct stevia plant designated as ‘12-05-149’ primarily adapted to produce sweetening agents.
  • the new plant is characterized by a distinct steviol glycoside profile.
  • Stevia variety ‘12-05-149’ is a green herbaceous perennial which grows best in high light conditions, in well-drained soil.
  • the new and distinct stevia plant ‘12-05-149’ originated from bi-parental controlled cross of two selections from an open-pollinated population of stevia in East Lansing, Mich.
  • Female parent ‘10-34’ (unpatented) was crossed with male parent ‘10-19’ (unpatented), in East Lansing, in 2012.
  • Stevia ‘ 12-05-149’ was selected from the plants resulting from the controlled cross of ‘10-34’ and ‘10-19’.
  • Stevia ‘ 12-05-149’ is distinct from female parent ‘10-34’ in that ‘12-05-149’ has lower stevioside concentration, higher rebaudioside A concentration, lower rebaudioside D concentration, higher rebaudioside M concentration, higher total steviol glycosides, higher rebaudioside A percentage (w/w of total steviol glycosides), lower rebaudioside D percentage (w/w of total steviol glycosides), and higher rebaudioside M percentage (w/w of total steviol glycosides) than female parent ‘10-34’.
  • Stevia ‘ 12-05-149’ is similar to female parent ‘10-34’ in that ‘12-05-149’ has similar rebaudioside C concentration to female parent ‘10-34’.
  • Stevia ‘ 12-05-149’ is distinct from male parent ‘10-19’ in that ‘12-05-149’ has lower stevioside concentration, higher rebaudioside A concentration, lower rebaudioside D concentration, higher rebaudioside M concentration, lower total steviol glycosides, higher rebaudioside A percentage (w/w of total steviol glycosides), lower rebaudioside D percentage (w/w of total steviol glycosides), and higher rebaudioside M percentage (w/w of total steviol glycosides) than male parent ‘10-19’.
  • Stevia ‘ 12-05-149’ is similar to male parent ‘10-19’ in that ‘12-05-149’ has similar rebaudioside C concentration to male parent ‘10-19’.
  • Stevia ‘ 12-05-005’ (co-pending application) is a selection from the same controlled cross of parent ‘10-34’ and parent ‘10-19’ from which ‘12-05-149’ was selected.
  • Stevia ‘ 12-05-149’ is distinct from comparator ‘12-05-005’ in that ‘12-05-149’ has lower stevioside concentration, lower rebaudioside A concentration, lower rebaudioside C concentration, lower rebaudioside M concentration, lower total steviol glycoside concentration, higher rebaudioside D percentage (w/w of total steviol glycosides), and higher rebaudioside M percentage (w/w of total steviol glycosides) than comparator ‘12-05-005’.
  • Stevia ‘ 12-05-149’ is similar to comparator plant ‘12-05-005’ in that ‘12-05-149’ has similar rebaudioside D concentration and similar rebaudioside A percentage (w/w of total steviol glycosides) to comparator plant ‘12-05-005’.
  • Stevia ‘ 12-05-144’ (co-pending application) is a selection from the same controlled cross of parent ‘10-34’ and parent ‘10-19’ from which ‘12-05-149’ was selected.
  • Stevia ‘ 12-05-149’ is distinct from comparator ‘12-05-144’ in that ‘12-05-149’ has lower stevioside concentration, lower rebaudioside A concentration, lower rebaudioside C concentration, lower rebaudioside M concentration, lower total steviol glycosides concentration, higher rebaudioside A percentage (w/w of total steviol glycosides), higher rebaudioside D percentage (w/w of total steviol glycosides), and lower rebaudioside M percentage (w/w of total steviol glycosides) than comparator ‘12-05-144’.
  • Stevia ‘ 12-05-149’ is similar to comparator ‘12-05-144’ in that ‘12-05-149’ has similar rebaudioside D concentration to comparator plant ‘12-05-144’.
  • the new and distinct stevia originated from a controlled cross of the stevia selection ‘10-34’ (female parent; unpatented) ⁇ stevia selection ‘10-19’ (male parent; unpatented). This cross was made and the resulting seedlings grown in East Lansing, Mich. The present seedling was selected from the controlled breeding program in 2012 and was designated ‘12-05-149’.
  • Stevia plant ‘12-05-149’ has been asexually propagated by cuttings since 2012 and was established in tissue culture in 2013 in East Lansing, Mich.
  • FIG. 1 Stevia plant in field.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

This invention relates to a new and distinct stevia plant designated as ‘12-05-149’ primarily adapted to produce sweeteners.

Description

Genus and species: Stevia rebaudiana.
Variety denomination: ‘12-05-149’.
BACKGROUND AND SUMMARY OF THE INVENTION
I. Field & Utility Summary
This invention relates to a new and distinct stevia plant designated as ‘12-05-149’ primarily adapted to produce sweetening agents. The new plant is characterized by a distinct steviol glycoside profile. Stevia variety ‘12-05-149’ is a green herbaceous perennial which grows best in high light conditions, in well-drained soil.
II. Cultivation Summary
The new and distinct stevia plant ‘12-05-149’ originated from bi-parental controlled cross of two selections from an open-pollinated population of stevia in East Lansing, Mich. Female parent ‘10-34’ (unpatented) was crossed with male parent ‘10-19’ (unpatented), in East Lansing, in 2012. Stevia ‘12-05-149’ was selected from the plants resulting from the controlled cross of ‘10-34’ and ‘10-19’.
III. Comparisons
Stevia ‘12-05-149’ Characteristics
The following traits have been repeatedly observed and are determined to be unique characteristics of ‘12-05-149’, which in combination distinguish this stevia plant as a new and distinct plant:
    • 1. low stevioside concentration
    • 2. medium-low rebaudioside A concentration
    • 3. medium rebaudioside C concentration
    • 4. low rebaudioside D concentration
    • 5. medium rebaudioside M concentration
    • 6. medium-low total steviol glycosides concentration
    • 7. high rebaudioside A percentage (w/w of total steviol glycosides)
    • 8. medium rebaudioside D percentage (w/w of total steviol glycosides)
    • 9. medium-high rebaudioside M percentage (w/w of total steviol glycosides)
Stevia ‘12-05-149’, parent ‘10-34’, parent ‘10-19’, comparator ‘12-05-005’, and comparator ‘12-05-144’ differ by the following combination of characteristics described in Table 1.
TABLE 1
% % %
Stevioside Reb A Reb C Reb D Reb M TSG Reb Reb Reb
Genotype (mg/g) (mg/g) (mg/g) (mg/g) (mg/g) (mg/g) A D M
12-05-144 7.07 57.09 5.07 5.16 5.33 79.72 71.6 6.47 6.69
12-05-005 7.26 62.99 5.42 5.42 4.25 85.34 73.8 6.35 4.98
12-05-149 3.66 47.08 4.11 5.47 3.88 64.20 73.3 8.52 6.04
10-19 13.46 44.02 4.13 7.94 2.67 72.23 60.9 11.00 3.70
10-34 11.67 35.95 4.14 6.34 1.90 60.01 59.9 10.56 3.17
Concentrations are in mg of compound per gram of dry leaf tissue;
TSG = total steviol glycosides
Comparator Female Parent ‘10-34’
Stevia ‘12-05-149’ is distinct from female parent ‘10-34’ in that ‘12-05-149’ has lower stevioside concentration, higher rebaudioside A concentration, lower rebaudioside D concentration, higher rebaudioside M concentration, higher total steviol glycosides, higher rebaudioside A percentage (w/w of total steviol glycosides), lower rebaudioside D percentage (w/w of total steviol glycosides), and higher rebaudioside M percentage (w/w of total steviol glycosides) than female parent ‘10-34’.
Stevia ‘12-05-149’ is similar to female parent ‘10-34’ in that ‘12-05-149’ has similar rebaudioside C concentration to female parent ‘10-34’.
Comparator Male Parent ‘10-19’
Stevia ‘12-05-149’ is distinct from male parent ‘10-19’ in that ‘12-05-149’ has lower stevioside concentration, higher rebaudioside A concentration, lower rebaudioside D concentration, higher rebaudioside M concentration, lower total steviol glycosides, higher rebaudioside A percentage (w/w of total steviol glycosides), lower rebaudioside D percentage (w/w of total steviol glycosides), and higher rebaudioside M percentage (w/w of total steviol glycosides) than male parent ‘10-19’.
Stevia ‘12-05-149’ is similar to male parent ‘10-19’ in that ‘12-05-149’ has similar rebaudioside C concentration to male parent ‘10-19’.
Comparator ‘12-05-005’
Stevia ‘12-05-005’ (co-pending application) is a selection from the same controlled cross of parent ‘10-34’ and parent ‘10-19’ from which ‘12-05-149’ was selected.
Stevia ‘12-05-149’ is distinct from comparator ‘12-05-005’ in that ‘12-05-149’ has lower stevioside concentration, lower rebaudioside A concentration, lower rebaudioside C concentration, lower rebaudioside M concentration, lower total steviol glycoside concentration, higher rebaudioside D percentage (w/w of total steviol glycosides), and higher rebaudioside M percentage (w/w of total steviol glycosides) than comparator ‘12-05-005’.
Stevia ‘12-05-149’ is similar to comparator plant ‘12-05-005’ in that ‘12-05-149’ has similar rebaudioside D concentration and similar rebaudioside A percentage (w/w of total steviol glycosides) to comparator plant ‘12-05-005’.
Comparator ‘12-05-144’
Stevia ‘12-05-144’ (co-pending application) is a selection from the same controlled cross of parent ‘10-34’ and parent ‘10-19’ from which ‘12-05-149’ was selected.
Stevia ‘12-05-149’ is distinct from comparator ‘12-05-144’ in that ‘12-05-149’ has lower stevioside concentration, lower rebaudioside A concentration, lower rebaudioside C concentration, lower rebaudioside M concentration, lower total steviol glycosides concentration, higher rebaudioside A percentage (w/w of total steviol glycosides), higher rebaudioside D percentage (w/w of total steviol glycosides), and lower rebaudioside M percentage (w/w of total steviol glycosides) than comparator ‘12-05-144’.
Stevia ‘12-05-149’ is similar to comparator ‘12-05-144’ in that ‘12-05-149’ has similar rebaudioside D concentration to comparator plant ‘12-05-144’.
IV. Breeding History
The new and distinct stevia originated from a controlled cross of the stevia selection ‘10-34’ (female parent; unpatented)×stevia selection ‘10-19’ (male parent; unpatented). This cross was made and the resulting seedlings grown in East Lansing, Mich. The present seedling was selected from the controlled breeding program in 2012 and was designated ‘12-05-149’.
V. Asexual Reproduction
Stevia plant ‘12-05-149’ has been asexually propagated by cuttings since 2012 and was established in tissue culture in 2013 in East Lansing, Mich.
VI. Stability
Asexual propagation as described has demonstrated that the combination of traits that characterize this plant are fixed and remain true to type through at least five successive generations.
BRIEF DESCRIPTION OF THE FIGURES
The accompanying color photographs show typical specimens of the new plant at various stages of development as nearly true as it is possible to make in color reproductions. Color in the photographs may differ slightly from the color value cited in the detailed botanical description, which accurately describes the color of ‘12-05-149’. The photograph was taken in East Lansing, Mich. in October 2014.
FIG. 1 Stevia plant in field.
 DETAILED DESCRIPTION OF THE INVENTION
The photographs together with the description of stevia ‘12-05-149’ are based upon the observations taken during the 2014 growing season in East Lansing, Mich. The following description of stevia plant ‘12-05-149’ contains references to color names taken from The Royal Horticultural Society Colour Chart (R.H.S.), 2001 edition. Botanical descriptions follow the Manual of Cultivated Plants (Bailey, 1949). Stevia plant ‘12-05-149’ has not been observed under all possible environmental conditions and as such the characteristics may vary in detail depending on weather conditions, day length, soil type and location.
  • Plant (average, at maturity):
      • Shape.—Upright to semi-upright.
      • Habit.—Herbaceous perennial.
      • Branching habit.—Freely branching basal nodes.
      • Number of nodes on main stem.—19.
      • Height.—363.8 mm.
      • Width.—203.2 mm.
      • Number of stems arising from base.—1.0.
      • Main stem length.—363.8 mm.
      • Main stem diameter at midpoint.—6.6.
      • Number of branches (arising from lower half of stem).—5.6.
      • Branch angle from main stem.—35°.
      • Main stem color.—Green 141B.
      • Stem length.—39 cm.
      • Stem diameter.—8.8 mm.
      • Internode length.—1.4 cm.
      • Stem texture.—Pubescent.
      • Stem color.—139D.
  • Leaves:
      • Shape.—Simple.
      • Arrangement on stem.—Generally opposite.
      • Margin.—Serrated.
      • Dorsal color.—Green 137A.
      • Ventral color.—Green 137C.
      • Venation color.—Green 137C.
      • Leaf angle from main stem.—82°.
      • Width.—11.1 mm.
      • Length, including petiole.—46.6.
      • Thickness.—<1 mm.
      • Length of petiole.—3.2 mm.
      • Petiole color.—Green 138B.
      • Petiole diameter.—Blade attaches directly to the stem.
      • Description of apex and base of leaf.—Minimal apex serration.
      • Texture of leaf (upper and lower surfaces).—Slightly pubescent.
      • Leaf length.—5.9 cm.
      • Leaf width.—1.4 cm.
  • Flowers:
      • Type.—Paniculate-corymbose.
      • Floret shape.—Tubular.
      • Stigma.—Exserted.
      • Inflorescence diameter.—5.0 mm.
      • Depth of inflorescence.—8.1 mm.
      • Number of florets per Inflorescence.—4.6.
      • Length of peduncle.—14.0 mm.
      • Number of sepals per floret.—0.
      • Number of petals per floret.—1.
      • Number of stigmas per floret.—2.
      • Floret diameter.—4.0 mm.
      • Bud color.—Green 138B.
      • Stigma color.—White 155A.
      • Petal color.—White 155A.
      • Sepal color.—Green 138B.
      • Basal buds.—4.
      • Blooming period.—October to killing frost.
      • Arrangement and fragrance of flower.—Corymb; non-fragrant.
  • Fruit:
      • Type.—Achenes.
  • Disease resistance and climate tolerance: Resistant to aphids (family Aphididae), tolerant to wind.

Claims (1)

What is claimed is:
1. A new and distinct stevia plant as herein described and illustrated.
US14/544,193 2014-12-08 2014-12-08 Stevia plant named ‘12-05-149’ Active 2035-04-20 USPP27939P3 (en)

Priority Applications (1)

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USPP27939P3 true USPP27939P3 (en) 2017-04-25

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USPP28373P3 (en) 2015-11-17 2017-09-12 S&W Seed Company Stevia plant named ‘SW 129’
USPP28977P3 (en) 2016-03-31 2018-02-20 S&W Seed Company Stevia plant named ‘SW 227’

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