US20230227810A1

US20230227810A1 - Methods for generating barcoded combinatorial libraries

Info

Publication number: US20230227810A1
Application number: US18/157,740
Authority: US
Inventors: Ryan T. Gill; Andrew Garst; Tanya Elizabeth Warnecke Lipscomb; Marcelo Colika BASSALO; Ramsey Ibrahim ZEITOUN
Original assignee: Muse Biotechnology Inc; Inscripta Inc; University of Colorado Colorado Springs
Current assignee: Muse Biotechnology Inc; Inscripta Inc; University of Colorado Colorado Springs
Priority date: 2016-06-24
Filing date: 2023-01-20
Publication date: 2023-07-20
Also published as: US20180230461A1; WO2017223538A9; AU2017280353B2; CA3029254A1; US20180230460A1; WO2017223538A1; US20190194650A1; US20170369870A1; CN109688820A; EP3474669B1; JP2019518478A; LT3474669T; DK3474669T3; EP3474669A1; US11584928B2; EP3474669A4; AU2017280353A1; ES2915562T3; CN109688820B; US10287575B2

Abstract

Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

Description

CROSS-REFERENCE

The present application claims priority to U.S. patent application Ser. No. 16/295,393, filed Mar. 7, 2019, which claims priority to U.S. patent application Ser. No. 15/948,798, filed Apr. 9, 2018, now U.S. Pat. No. 10,287,575; which claims priority to U.S. patent application Ser. No. 15/948,793, filed Apr. 9, 2018, now U.S. Pat. No. 10,294,473; which claims priority to U.S. patent application Ser. No. 15/632,222, filed Jun. 23, 2017, now U.S. Pat. No. 10,017,760; which claims priority to U.S. Provisional Application Ser. No. 62/354,516, filed Jun. 24, 2016; U.S. Provisional Application Ser. No. 62/367,386, filed Jul. 27, 2016; and U.S. Provisional Application Ser. No. 62/483,930, filed Apr. 10, 2017, the contents of each being hereby incorporated by reference in their entirety.

STATEMENT AS TO FEDERALLY SPONSORED RESEARCH

This disclosure was made with the support of the United States government under Contract number DE-SC0008812 by the Department of Energy.

SEQUENCE LISTING

The instant application contains a Sequence Listing which has been submitted in XML file format via EFS-Web and is hereby incorporated by reference in its entirety. Said XML copy, created Mar. 6, 2023, is named 14325_002US5_SL.xml and is 666,805 bytes in size.

BACKGROUND OF THE DISCLOSURE

Understanding the relationship between a protein's amino acid structure and its overall function continues to be of great practical, clinical, and scientific significance for biologists and engineers. Directed evolution can be a powerful engineering and discovery tool, but the random and often combinatorial nature of mutations makes their individual impacts difficult to quantify and thus challenges further engineering. More systematic analysis of contributions of individual residues or saturation mutagenesis remains labor- and time-intensive for entire proteins and simply is not possible on reasonable timescales for editing of multiple proteins in parallel, such as metabolic pathways or multi-protein complexes, using standard methods.

SUMMARY OF THE DISCLOSURE

Disclosed herein are compositions comprising: i) a first donor nucleic acid comprising: a) a modified first target nucleic acid sequence; b) a first protospacer adjacent motif (PAM) mutation; and c) a first guide nucleic acid sequence comprising a first spacer region complementary to a portion of the first target nucleic acid; and ii) a second donor nucleic acid comprising: a) a barcode corresponding to the modified first target nucleic acid sequence; and b) a second guide nucleic acid sequence comprising a second spacer region complementary to a portion of a second target nucleic acid. Further disclosed are compositions wherein the modified first target nucleic acid sequence comprises at least one inserted, deleted, or substituted nucleic acid compared to a corresponding un-modified first target nucleic acid. Further disclosed are compositions wherein the first guide nucleic acid and second guide nucleic acid are compatible with a nucleic acid-guided nuclease. Further disclosed are compositions wherein the nucleic acid-guided nuclease is a Type II or Type V Cas protein. Further disclosed are compositions wherein the nucleic acid-guided nuclease is a Cas9 homologue or a Cpf1 homologue. Further disclosed are compositions wherein the second donor nucleic acid comprises a second PAM mutation. Further disclosed are compositions wherein the second donor nucleic acid sequence comprises a regulatory sequence or a mutation to turn a screenable or selectable marker on or off. Further disclosed are compositions wherein the second donor nucleic acid sequence targets a unique landing site.
Disclosed herein are methods of genome engineering, the method comprising: a) contacting a population of cells with a polynucleotide, wherein each cell comprises a first target nucleic acid, a second target nucleic acid, and a nucleic acid-guided nuclease, wherein the polynucleotide comprises 1) an editing cassette comprising: i) a modified first target nucleic acid sequence; ii) a first protospacer adjacent motif (PAM) mutation; iii) a first guide nucleic acid sequence comprising a spacer region complementary to a portion of the first target nucleic acid and compatible with the nucleic acid-guided nuclease; and 2) a recorder cassette comprising i) a barcode corresponding to the modified first target nucleic acid sequence; and ii) a second guide nucleic acid sequence comprising a second spacer region complementary to a portion of the second target nucleic acid and compatible with the nucleic acid-guided nuclease; b) allowing the first guide nucleic acid sequence, the second guide nucleic acid sequence, and the nucleic acid-guided nuclease to create a genome edit within the first target nucleic acid and the second target nucleic acid. Further disclosed are methods further comprising c) sequencing a portion of the barcode, thereby identifying the modified first target nucleic acid that was inserted within the first target nucleic acid in step a). Further disclosed are methods wherein the nucleic acid-guided nuclease is a CRISPR nuclease. Further disclosed are methods wherein the PAM mutation is not recognized by the nucleic acid-guided nuclease. Further disclosed are methods wherein the nucleic acid-guided nuclease is a Type II or Type V Cas protein. Further disclosed are methods wherein the nucleic acid-guided nuclease is a Cas9 homologue or a Cpf1 homologue. Further disclosed are methods wherein the recorder cassette further comprises a second PAM mutation that is not recognized by the nucleic acid-guided nuclease.
Disclosed herein are methods of selectable recursive genetic engineering comprising a) contacting cells comprising a nucleic acid-guided nuclease with a polynucleotide comprising a recorder cassette, said recorder cassette comprising i) a nucleic acid sequence that recombines into a unique landing site incorporated during a previous round of engineering, wherein the nucleic acid sequence comprises a unique barcode; and ii) a guide RNA compatible with the nucleic acid-guided nuclease that targets the unique landing site; and b) allowing the nucleic acid-guided nuclease to edit the unique landing site, thereby incorporating the unique barcode into the unique landing site. Further disclosed are methods wherein the nucleic acid sequence further comprises a regulatory sequence that turns transcription of a screenable or selectable marker on or off. Further disclosed are methods wherein the nucleic acid sequence further comprises a PAM mutation that is not compatible with the nucleic acid-guided nuclease. Further disclosed are methods wherein the nucleic acid sequence further comprises a second unique landing site for subsequent engineering rounds. Further disclosed are methods wherein the polynucleotide further comprises an editing cassette comprising a) a modified first target nucleic acid sequence; b) a first protospacer adjacent motif (PAM) mutation; and c) a first guide nucleic acid sequence comprising a first spacer region complementary to a portion of the first target nucleic acid, wherein the unique barcode corresponds to the modified first target nucleic acid such that the modified target nucleic acid can be identified by the unique barcode.
Provided herein are compositions comprising i) a first donor nucleic acid comprising: a) a modified first target nucleic acid sequence; b) a mutant protospacer adjacent motif (PAM) sequence; and c) a first guide nucleic acid sequence comprising a first spacer region complementary to a portion of the first target nucleic acid; and ii) a second donor nucleic acid comprising: a) a recorder sequence; and b) a second guide nucleic acid sequence comprising a second spacer region complementary to a portion of the second target nucleic acid. In some aspects, the first donor nucleic acid and the second donor nucleic acid are covalently linked or comprised on a single nucleic acid molecule. Further provided are compositions wherein the modified first target nucleic acid comprises a 5′ homology are and a 3′ homology arm. Further provided are compositions wherein the 5′ homology arm and the 3′ homology arm are homologous to nucleic acid sequence flanking a protospacer complementary to the first spacer region. Further provided are compositions wherein the modified first target nucleic acid sequence comprises at least one inserted, deleted, or substituted nucleic acid compared to a corresponding un-modified first target nucleic acid. Further provided are compositions wherein the first gRNA is compatible with a nucleic acid-guided nuclease, thereby facilitating nuclease-mediate cleavage of the first target nucleic acid. Further provided are compositions wherein the nucleic acid-guided nuclease is a Cas protein, such as a Type II or Type V Cas protein. Further provided are compositions wherein the nucleic acid-guided nuclease is Cas9 or Cpf1. Further provided are compositions wherein the nucleic acid-guided nuclease is MAD2 or MAD7. Further provided are compositions wherein the nucleic acid-guided nuclease is an engineered or non-natural enzyme. Further provided are compositions wherein the nucleic acid-guided nuclease is a engineered or non-natural enzyme derived from Cas9 or Cpf1. Further provided are compositions wherein the nucleic acid-guided nuclease is an engineered or non-natural enzyme that has less than 80% homology to either Cas9 or Cpf1. Further provided are compositions wherein the mutant PAM sequence is not recognized by the nucleic acid-guided nuclease. Further provided are compositions wherein the recorder sequence comprises a barcode. Further provided are compositions wherein the recorder sequence comprises a fragment of a screenable or selectable marker. Further provided are compositions wherein the recorder sequence comprises a unique sequence by which the modified first target nucleic acid sequence is specifically identified. Further provided are compositions wherein the recorder sequence comprises a unique sequence by which the edited cells may be selected or enriched. A first donor nucleic acid can be a cassette, such as an editing cassette as disclosed herein. A second donor nucleic acid can be a cassette, such as a recording cassette as disclosed herein. A first donor nucleic acid and a second donor nucleic acid can be comprised on a single cassette. A first donor nucleic acid and a second donor nucleic acid can be covalently linked. In any of these examples, the elements of the cassette or donor nucleic acids can be contiguous or non-contiguous.
Provided herein are cells comprising an engineered chromosome or polynucleic acid comprising: a first modified sequence; a first mutant protospacer adjacent motif (PAM); a first recorder sequence, the sequence of which uniquely identifies the first modified sequence, wherein the first modified sequence and the first recorder sequence are separated by at least 1 bp. Further provided are cells wherein the first modified sequence and the first recorder sequence are separated by at least 100 bp. Further provided are cells wherein the first modified sequence and the first recorder sequence are separated by at least 500 bp. Further provided are cells wherein the first modified sequence and the first recorder sequence are separated by at least lkbp. Further provided are cells wherein the first recorder sequence is a barcode. Further provided are cells wherein the first modified sequence is within a coding sequence. Further provided are cells wherein the first modified sequence comprises at least one inserted, deleted, or substituted nucleotide compared to an unmodified sequence. Further provided are cells further comprising: a second modified sequence; a second mutant PAM; and a second recorder sequence, the sequence of which uniquely identifies the second modified sequence, wherein the second modified sequence and the second recorder sequence are separated by at least 1 kb. Further provided are cells wherein the first recorder sequence and the second recorder sequence are separated by less than 100 bp. Further provided are cells wherein the second recorder sequence is a barcode. Further provided are cells wherein the second modified sequence is within a coding sequence. Further provided are cells wherein the second modified sequence comprises at least one inserted, deleted, or substituted nucleotide compared to an unmodified sequence. Further provided are cells wherein the first recorder sequence and the second recorder sequence are immediately adjacent to each other or overlapping, thereby generating a combined recorder sequence. Further provided are cells wherein the combined recorder sequence comprises a selectable or screenable marker. Further provided are cells wherein the combined recorder sequence comprises a selectable or screenable marker by which the cells may be enriched or selected.
Provided herein are methods of genome engineering, the method comprising: a) introducing into a population of cells a plurality of polynucleotides, wherein each cell comprises a first target nucleic acid, a second target nucleic acid, and a targetable nuclease, wherein each polynucleotide comprises: i) a modified first target nucleic acid sequence; ii) a mutant protospacer adjacent motif (PAM) sequence; iii) a first guide nucleic acid sequence comprising a guide sequence complementary to a portion of the first target nucleic acid; and (iv) a recorder sequence; b) inserting the modified first target nucleic acid sequence within the first target nucleic acid; c) inserting the recorder sequence within the second target nucleic acid; d) cleaving the first target nucleic acid by the targetable nuclease in cells that do not comprise the mutant PAM sequence, thereby enriching for cells comprising the inserted modified first target nucleic acid sequence. Further provided are methods wherein the recorder sequence is linked to the modified first target nucleic acid. Further provided are methods wherein each polynucleotide further comprises a second mutant PAM sequence. Further provided are methods wherein each polynucleotide further comprises a second guide nucleic acid sequence comprising a guide sequence complementary to a portion of the second target nucleic acid. Further provided are methods wherein the recorder sequence comprises a unique sequence by which the modified first target nucleic acid is specifically identified upon sequencing the recorder sequence. Further provided are methods further comprising e) sequencing the recorder sequence, thereby identifying the modified first target nucleic acid that was inserted within the first target nucleic acid in step b). Further provided are methods wherein inserting the modified first target nucleic acid sequence comprises cleaving the first target nucleic acid by the nuclease complexed with the transcription product of the first guide nucleic acid sequence. Further provided are methods wherein inserting the modified first target nucleic acid sequence further comprises homology-directed repair. Further provided are methods wherein inserting the modified first target nucleic acid sequence further comprises homologous recombination. Further provided are methods wherein the polynucleotide further comprises a second guide nucleic acid sequence comprising a spacer region complementary to a portion of the second target nucleic acid. Further provided are methods wherein inserting the recorder sequence comprises cleaving the second target nucleic acid by the nuclease complexed with the transcription product of the second guide nucleic acid sequence. Further provided are methods wherein inserting the modified first target nucleic acid sequence further comprises homology-directed repair. Further provided are methods wherein inserting the modified first target nucleic acid sequence further comprises homologous recombination. Further provided are methods wherein the targetable nuclease is a Cas protein. Further provided are methods wherein the Cas protein is a Type II or Type V Cas protein. Further provided are methods wherein the Cas protein is Cas9 or Cpf1. Further provided are methods wherein the targetable nuclease is a nucleic acid-guided nuclease. Further provided are methods wherein the targetable nuclease is MAD2 or MAD7. Further provided are methods wherein the mutant PAM sequence is not recognized by the targetable nuclease. Further provided are methods wherein the targetable nuclease is an engineered targetable nuclease. Further provided are methods wherein the mutant PAM sequence is not recognized by the engineered targetable nuclease. Further provided are methods further comprising introducing a second plurality of polynucleotides into a second population of cells comprising the enriched cells from step d), wherein each cell within the second population of cells comprises a third nucleic acid, a fourth target nucleic acid, and a targetable nuclease. Further provided are methods wherein each of the second polynucleotides comprises: i) a modified third target nucleic acid sequence; ii) a third mutant protospacer adjacent motif (PAM) sequence; iii) a third guide nucleic acid sequence comprising a spacer region complementary to a portion of the third target nucleic acid; and (iv) a second recorder sequence. Further provided are methods wherein each second polynucleotide further comprises a fourth mutant PAM sequence. Further provided are methods wherein each second polynucleotide further comprises a fourth guide nucleic acid sequence comprising a guide sequence complementary to a portion of the fourth target nucleic acid. Further provided are methods further comprising: a) inserting the modified third target nucleic acid sequence within the third target nucleic acid; b) inserting the second recorder sequence within the fourth target nucleic acid; c) cleaving the third target nucleic acid by the nuclease in cells that do not comprise the second mutant PAM sequence, thereby enriching for cells comprising the inserted modified third target nucleic acid sequence. Further provided are methods wherein the fourth target nucleic acid is adjacent to the second target nucleic acid. Further provided are methods wherein the inserted first recorder sequence is adjacent to the second recorder sequence, such that sequencing information can be obtained for the first and second recorder sequence from a single sequencing read. Further provided are methods further comprising obtaining sequence information from the first and second recorder sequences within a single sequence read, thereby identifying the modified first and third target nucleic acid sequences inserted into the first and third target nucleic acids respectively.
Provided herein are methods of identifying engineered cells, the method comprising: a) providing cells, wherein each cell comprises a first target nucleic acid, a second target nucleic acid, and a targetable nuclease, b) introducing into the cells a polynucleotide comprising: 1) a first donor nucleic acid comprising i) a modified target nucleic acid sequence; ii) a mutant protospacer adjacent motif (PAM) sequence; and iii) a first guide nucleic acid sequence comprising a first guide sequence complementary to a portion of the first target nucleic acid; and 2) a second donor nucleic acid comprising i) a recorder sequence corresponding to the modified target nucleic acid sequence; and ii) a second guide nucleic acid sequence comprising a second guide sequence complementary to a portion of the second target nucleic acid, c) cleaving the first target nucleic acid by the nuclease in cells that do not comprise the mutant PAM sequence, thereby enriching for cells comprising the modified target nucleic acid sequence, d) repeating steps a)-c) at least one time using the cells enriched for in step c) as the cells for step a) of the following round, wherein the recorder sequence from each round is incorporated adjacent to the recorder sequence from the previous round, thereby generating a record sequence array comprising a plurality of traceable barcodes, and e) sequencing the record sequence, thereby identifying engineered cells comprising a desired combination of modified target nucleic acids. Further provided are methods wherein the second donor nucleic acid further comprises a second mutant PAM sequence. Further provided are methods wherein sequencing the record sequence array comprises obtaining sequence information for each of the plurality of recorder sequences within a single sequencing read. Further provided are methods wherein steps a)-c) are repeated at least once. Further provided are methods wherein steps a)-c) are repeated at least twice. Further provided are methods wherein the recorder sequence is a barcode. Further provided are methods where the first donor nucleic acid and the second donor nucleic acid are covalently linked. A first donor nucleic acid can be a cassette, such as an editing cassette as disclosed herein. A second donor nucleic acid can be a cassette, such as a recording cassette as disclosed herein. A first donor nucleic acid and a second donor nucleic acid can be comprised on a single cassette. A first donor nucleic acid and a second donor nucleic acid can be covalently linked. In any of these examples, the elements of the cassette or donor nucleic acids can be contiguous or non-contiguous.
Provided herein are methods of identifying engineered cells, the method comprising: a) providing cells, wherein each cell comprises a first target nucleic acid, a second target nucleic acid, and a targetable nuclease, b) introducing into the cells a polynucleotide comprising: 1) a first donor nucleic acid comprising i) a modified target nucleic acid sequence; ii) a mutant protospacer adjacent motif (PAM) sequence; and iii) a first guide nucleic acid sequence comprising a first guide sequence complementary to a portion of the first target nucleic acid; and 2) a second donor nucleic acid comprising i) a marker fragment corresponding to the modified target nucleic acid sequence; and ii) a second guide nucleic acid sequence comprising a second guide sequence complementary to a portion of the second target nucleic acid, c) cleaving the first target nucleic acid by the nuclease in cells that do not comprise the mutant PAM sequence, thereby enriching for cells comprising the modified target nucleic acid sequence, d) repeating steps a)-c) at least one time using the cells enriched for in step c) as the cells for step a) of the following round, wherein the marker fragment from each round is incorporated adjacent to the marker fragment from the previous round, thereby generating a complete marker, and e) identifying cells comprising the complete marker, thereby identifying engineered cells comprising a desired combination of modified target nucleic acids. Further provided are methods wherein the second donor nucleic acid further comprises a second mutant PAM sequence. Further provided are methods wherein the complete marker comprises a selectable marker. Further provided are methods wherein the selectable marker comprises an antibiotic resistance marker or an auxotrophic marker. Further provided are methods wherein the complete marker comprises a screenable reporter. Further provided are methods wherein the screenable reporter comprises a fluorescent reporter. Further provided are methods wherein the screenable reporter comprises a gene. Further provided are methods wherein the screenable reporter comprises a promotor or regulatory element. Further provided are methods wherein the promoter or regulatory element turns on or off transcription of a screenable or selectable element. Further provided are methods wherein the screenable reporter comprises a screenable or selectable element which alters a characteristic of a colony comprising the element compared to a colony that does not comprise the element. A first donor nucleic acid can be a cassette, such as an editing cassette as disclosed herein. A second donor nucleic acid can be a cassette, such as a recording cassette as disclosed herein. A first donor nucleic acid and a second donor nucleic acid can be comprised on a single cassette. A first donor nucleic acid and a second donor nucleic acid can be covalently linked. In any of these examples, the elements of the cassette or donor nucleic acids can be contiguous or non-contiguous.
Provided herein are methods of genome engineering, the method comprising: a) introducing into a population of cells a polynucleotide, wherein each cell comprises a first target nucleic acid, a second target nucleic acid, and a targetable nuclease, wherein the polynucleotide comprises: i) a modified first target nucleic acid sequence; ii) a mutant nuclease recognition sequence; iii) a recorder sequence; b) inserting the modified first target nucleic acid sequence within the first target nucleic acid; c) inserting the recorder sequence within the second target nucleic acid; and d) selecting for a phenotype of interest. Further provided are methods wherein the polynucleotide further comprises a second mutant nuclease recognition site. Further provided are methods wherein selecting for a phenotype of interest comprises cleaving the first target nucleic acid by the nuclease in cells that do not comprise the mutant nuclease recognition sequence, thereby enriching for cells comprising the inserted modified first target nucleic acid sequence. Further provided are methods wherein selecting for a phenotype of interest comprises cleaving the second target nucleic acid by the nuclease in cells that do not comprise the second mutant nuclease recognition sequence, thereby enriching for cells comprising the inserted modified first target nucleic acid sequence. Further provided are methods wherein the recorder sequence is linked to the modified first target nucleic acid. Further provided are methods wherein the recorder sequence comprises a unique sequence by which the modified first target nucleic acid is specifically identified upon sequencing the recorder sequence. Further provided are methods further comprising e) sequencing the recorder sequence, thereby identifying the modified first target nucleic acid that was inserted within the first target nucleic acid in step b). Further provided are methods wherein inserting the modified first target nucleic acid sequence comprises homology-directed repair. Further provided are methods wherein inserting the modified first target nucleic acid sequence comprises homologous recombination. Further provided are methods wherein the nuclease is a Cas protein. Further provided are methods wherein the polynucleotide further comprises a first guide nucleic acid sequence comprising a guide sequence complementary to a portion of the first target nucleic acid. Further provided are methods wherein inserting the modified first target nucleic acid sequence comprises cleaving the first target nucleic acid by the nuclease complexed with the transcription product of the first guide nucleic acid sequence. Further provided are methods wherein the polynucleotide further comprises a second guide nucleic acid sequence comprising a guide sequence complementary to a portion of the second target nucleic acid. Further provided are methods wherein inserting the recorder sequence comprises cleaving the second target nucleic acid by the nuclease complexed with the transcription product of the second guide nucleic acid sequence. Further provided are methods wherein inserting the modified first target nucleic acid sequence or the recorder sequence comprises homology-directed repair. Further provided are methods wherein inserting the modified first target nucleic acid sequence or the recorder sequence comprises homologous recombination. Further provided are methods wherein the mutant nuclease recognition sequence comprises a mutant PAM sequence not recognized by the targetable nuclease. Further provided are methods wherein the Cas protein is a Type II or Type V Cas protein. Further provided are methods wherein the targetable nuclease is MAD2. Further provided are methods wherein the mutant PAM sequence is not recognized by MAD2. Further provided are methods wherein the targetable nuclease is MAD7. Further provided are methods wherein the mutant PAM sequence is not recognized by MAD7. Further provided are methods wherein the Cas protein is Cas9. Further provided are methods wherein the mutant PAM sequence is not recognized by Cas9. Further provided are methods wherein the Cas protein is Cpf1. Further provided are methods wherein the mutant PAM sequence is not recognized by Cpf1. Further provided are methods wherein the nuclease is an Argonaute nuclease. Further provided are methods further comprising introducing guide DNA oligonucleotides comprising a guide sequence complementary to a portion of the first target nucleic acid prior to selecting for a phenotype. Further provided are methods wherein the mutant nuclease recognition sequence comprises a mutant target flanking sequence not recognized by the Argonaute nuclease. Further provided are methods wherein the nuclease is a zinc finger nuclease. Further provided are methods wherein the mutant nuclease recognition sequence is not recognized by the zinc finger nuclease. Further provided are methods wherein the nuclease is a transcription activator-like effector nuclease (TALEN). Further provided are methods wherein the mutant nuclease recognition sequence is not recognized by the TALEN.

INCORPORATION BY REFERENCE

All publications and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference.

BRIEF DESCRIPTION OF THE DRAWINGS

The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.

FIGS. 1A-1C depict an example genetic engineering workflow including target design, plasmid design, and plasmid library generation. Figure discloses SEQ ID NOS 187-190, respectively, in order of appearance.

FIGS. 2A-2D depicts validation data for an example experiment using a disclosed engineering method.

FIGS. 3A-3C depict an example trackable genetic engineering workflow, including a plasmid comprising an editing cassette and a recording cassette, and downstream sequencing of barcodes in order to identify the incorporated edit or mutation. Figure discloses SEQ ID NOS 191-192, respectively, in order of appearance.

FIGS. 3D-3E depict an example trackable genetic engineering workflow, including iterative rounds of engineering with a different editing cassette and recorder cassette with unique barcode (BC) at each round, followed by selection and tracking to confirm the successful engineering step at each round.

FIGS. 4A-4B depict an example of incorporation of a target mutation and PAM mutation using a plasmid comprising an editing cassette. Figure discloses SEQ ID NOS 193, 193, 194, 193, 194, 193, 193, 195, 193, 196, 193, 197, 194, 193, and 198, respectively, in order of appearance.

FIGS. 5A-5B depict an example of a plasmid comprising an editing cassette, designed to incorporate a target mutation and a PAM mutation into a first target sequence, and a recording cassette, designed to incorporate a barcode sequence into a second target sequence. FIG. 5B depicts example data validating incorporation of the editing cassette and recorder cassette and selection of the engineered bacterial cells. FIG. 5A discloses the left column sequences as

SEQ ID NOS

201, 200, 201, 200, 200, 200, 200, 200, 200, 200, 200, 201, 202, 200, 200, 200, 200, 200, 200, 200, 202, and 200, respectively, in order of appearance and the right column sequences as SEQ ID NOS 203, 204, 204, 204, 204, 204, 204, 204, 204, 204, 204, 203, 205, 205, 205, 205, 205, 205, 205, 205, 205, and 205, respectively, in order of appearance.

FIG. 6 depicts an example recursive engineering workflow.

FIGS. 7A-7B depict an example plasmid curing workflow for combinatorial engineering and validation of an example experiment using said workflow.

FIGS. 8A-8B depict an example genetic engineering workflow including target design, plasmid design, and plasmid library generation. Figure discloses SEQ ID NOS 187-190, respectively, in order of appearance.

FIGS. 9A-9D depicts validation data for an example genetic engineering experiment.

FIGS. 10A-10F depict an example data set from a genetic engineering experiment.

FIGS. 11A-11C depict an example design and data set from a genetic engineering experiment.

FIGS. 12A-12F depict an example design for a genetic engineering experiment.

FIGS. 13A-13D depict example designed edits to be made by a genetic engineering. Figure discloses SEQ ID NOS 187-190 and 206-207, respectively, in order of appearance.

FIGS. 14A-14B depict an example design for a genetic engineering experiment.

FIGS. 15A-15D depict an example of Cas9 editing efficiency controls. Figure discloses SEQ ID NOS 208-209, respectively, in order of appearance.

FIGS. 16A-16E depict an examples of toxicity of dsDNA cleavage in E. coli.

FIG. 16F-16H depict an example of a transformation and survival assay, and editing and recording efficiencies, with low and high copy plasmids expressing Cas9.

FIGS. 17A-17D depict an example of genetic engineering strategy for gene deletion. FIGS. 17A and 17C disclose SEQ ID NO: 210.

FIGS. 18A-18B depicts an example of editing efficiency controls by cotransformation of guide nucleic acid and linear dsDNA cassettes.

FIGS. 19A-19D depict an example of library cloning analysis and statistics.

FIGS. 20A-20B depict an example of precision of editing cassette tracking of recombineered populations.

FIG. 21 depicts an example of growth characteristics of folA mutations in M9 minimal media

FIGS. 22A-22C depicts an example of enrichment profiles for folA editing cassettes in minimal media.

FIGS. 23A-23F depict an example of validation of identified acrB mutations for improved solvent and antibiotic tolerance.

FIGS. 24A-24D depict an example mutant variant assessment analysis.

FIG. 25 depicts an example of reconstruction of mutations identified by erythromycin selection.

FIGS. 26A-26B depict an example of validation of Crp S28P mutation for furfural or thermal tolerance.

FIGS. 27A-27C depict an example of edit and barcode correlation studies.

FIG. 28 depicts an example of a selectable recording strategy.

FIG. 29 depicts an example of a selectable recording strategy.

FIGS. 30A-30B depict data from a selectable recording experiment. Figure discloses “TCCACTGGTATGCAT” as SEQ ID NO: 211.

FIGS. 31A-31B depict editing and transformation efficiencies from various nucleic acid-guided nucleases from an example experiment.

FIG. 32 depict editing efficiencies of the MAD2 nuclease with various guide nucleic acids.

FIG. 33 depict editing efficiencies of the MAD7 nuclease with various guide nucleic acids.

DETAILED DESCRIPTION OF THE DISCLOSURE

While preferred embodiments of the present invention have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the invention. It should be understood that various alternatives to the embodiments of the invention described herein may be employed in practicing the invention.
Methods and compositions for enabling sophisticated combinatorial engineering strategies to optimize and explore complex phenotypes are provided herein. Many phenotypes of interest to basic research and biotechnology are the result of combinations of mutations that occur at distal loci. For example, cancer is often linked to mutations that influence multiple hallmark gene functions rather than a single chromosomal edit. Likewise, many metabolic and regulatory processes that are the target of continuing engineering efforts require the activities of many proteins acting in concert to produce the phenotypic output of interest. Methods and compositions disclosed herein can provide ways of rapid engineering and prototyping of such functions since they can provide rapid construction and accurate reporting on the mutational effects at many sites in parallel.
The methods and compositions described herein can be carried out or used in any type of cell in which a nucleic acid-guided nuclease system, such as CRISPR or Argonaute, or other targetable nuclease systems, such as TALEN, ZFN, or meganuclease can function (e.g., target and cleave DNA), including prokaryotic, eukaryotic, or archaeal cells. The cell can be a bacterial cell, such as Escherichia spp. (e.g., E. coli). The cell can be a fungal cell, such as a yeast cell, e.g., Saccharomyces spp. The cell can be a human cell. The cell can be an algal cell, a plant cell, an insect cell, or a mammalian cell, including a human cell. Additionally or alternatively, the methods described herein can be carried out in vitro or in cell-free systems in which a nucleic acid guided nuclease system, such as CRISPR or Argonaute, or other nuclease systems, such as TALEN, ZFN, or meganuclease can function (e.g., target and cleave DNA).
Disclosed herein are compositions and methods for genetic engineering. Disclosed are methods and compositions suitable for trackable or recursive genetic engineering. Disclosed method and compositions can use massively multiplexed oligonucleotide synthesis and cloning to enable high fidelity, trackable, multiplexed genome editing at single nucleotide resolution on a whole genome scale.

Trackable Plasmids

Methods and compositions can be used to perform high-fidelity trackable editing, for example, at single-nucleotide resolution and can be used to perform editing at a whole genome scale or on episomal nucleic acid molecules. Massively multiplexed oligonucleotide synthesis and/or cloning can be used in combination with a targetable nuclease system, such as a CRISPR system, MAD2 system, MAD7 system, or other nucleic acid-guided nuclease system, for editing.
As used herein, “cassette” often refers to a single molecule polynucleotide. A cassette can comprise DNA. A cassette can comprise RNA. A cassette can comprise a combination of DNA and RNA. A cassette can comprise non-naturally occurring nucleotides or modified nucleotides. A cassette can be single stranded. A cassette can be double stranded. A cassette can be synthesized as a single molecule. A cassette can be assembled from other cassettes, oligonucleotides, or other nucleic acid molecules. A cassette can comprise one or more elements. Such elements can include, as non-limiting examples, one or more of any of editing sequences, recorder sequences, guide nucleic acids, promoters, regulatory elements, mutant PAM sequences, homology arms, primer sites, linker regions, unique landing sites, a cassette, and any other element disclosed herein. Such elements can be in any order or combination. Any two or more elements can be contiguous or non-contiguous. A cassette can be comprised within a larger polynucleic acid. Such a larger polynucleic acid can be linear or circular, such as a plasmid or viral vector. A cassette can be a synthesized cassette. A cassette can be a trackable cassette.
A cassette can be designed to be used in any method or composition disclosed herein, including multiplex engineering methods and trackable engineering methods. An exemplary cassette can couple two or more elements, such as 1) a guide nucleic acid (e.g. gRNAs or gDNAs) designed for targeting a user specified target sequence in the genome and 2) an editing sequence and/or recorder sequence as disclosed herein (e.g. FIG. 1B and FIG. 5A). A cassette comprising an editing sequence and guide nucleic acid can be referred to as an editing cassette. A cassette comprising an editing sequence can be referred to as an editing cassette. A cassette comprising a recorder sequence and a guide nucleic acid can be referred to as a recorder cassette. A cassette comprising a recorder sequence can be referred to as a recorder cassette. In a preferred embodiment, an editing cassette and a recorder cassette are delivered into the cell at the same time. Further, an editing cassette and a recorder cassette may be covalently linked. Further, these elements may be synthesized together by multiplexed oligonucleotide synthesis.
A cassette can comprise one or more guide nucleic acids and editing cassette as a contiguous polynucleotide. In other examples, one or more guide nucleic acids and editing cassette are contiguous. In other examples, one or more guide nucleic acids and editing cassette are non-contiguous. In other examples, two or more guide nucleic acids and editing cassette are non-contiguous.
A cassette can comprise one or more guide nucleic acids, an editing cassette, and a recorder cassette as a contiguous polynucleotide. In other examples, one or more guide nucleic acids, editing cassette, and recorder cassette are contiguous. In other examples, two or more guide nucleic acids, editing cassette, and recorder cassette are contiguous. In other examples, one or more guide nucleic acids, editing cassette, and recorder cassette are non-contiguous. In other examples, two or more guide nucleic acids, editing cassette, and recorder cassette are non-contiguous.
A cassette can comprise one or more guide nucleic acids, one or more editing cassettes, and one or more recorder cassettes as a contiguous polynucleotide. In other examples, one or more guide nucleic acids, one or more editing cassettes, and one or more recorder cassettes are contiguous. In other examples, two or more guide nucleic acids, two or more editing cassettes, and two or more recorder cassettes are contiguous. In other examples, one or more guide nucleic acids, one or more editing cassettes, and one or more recorder cassettes are non-contiguous. In other examples, two or more guide nucleic acids, two or more editing cassettes, and two or more recorder cassettes are non-contiguous.
A cassette can comprise one or more guide nucleic acids and editing sequence as a contiguous polynucleotide. In other examples, one or more guide nucleic acids and editing sequence are contiguous. In other examples, one or more guide nucleic acids and editing sequence are non-contiguous. In other examples, two or more guide nucleic acids and editing sequence are non-contiguous.
A cassette can comprise one or more guide nucleic acids, an editing sequence, and a recorder sequence as a contiguous polynucleotide. In other examples, one or more guide nucleic acids, editing sequence, and recorder sequence are contiguous. In other examples, two or more guide nucleic acids, editing sequence, and recorder sequence are contiguous. In other examples, one or more guide nucleic acids, editing sequence, and recorder sequence are non-contiguous. In other examples, two or more guide nucleic acids, editing sequence, and recorder sequence are non-contiguous.
A cassette can comprise one or more guide nucleic acids, one or more editing sequences, and one or more recorder sequences as a contiguous polynucleotide. In other examples, one or more guide nucleic acids, one or more editing sequences, and one or more recorder sequences are contiguous. In other examples, two or more guide nucleic acids, two or more editing sequences, and two or more recorder sequences are contiguous. In other examples, one or more guide nucleic acids, one or more editing sequences, and one or more recorder sequences are non-contiguous. In other examples, two or more guide nucleic acids, two or more editing sequences, and two or more recorder sequences are non-contiguous.
An editing cassette can comprise an editing sequence. An editing sequence can comprise a mutation, such as a synonymous or non-synonymous mutation, and homology arms (HAs). An editing sequence can comprise a mutation, such as a synonymous or non-synonymous mutation, and homology arms (HAs) designed to undergo homologous recombination with the target sequence at the site of nucleic acid-guided nuclease-mediated double strand break (e.g. FIG. 1B).
A recorder cassette can comprise a recorder sequence. A recorder sequence can comprise a trackable sequence, such as a barcode or marker, and homology arms (HAs). A recorder sequence can comprise a trackable sequence, such as a barcode or marker, and homology arms (HAs) designed to undergo homologous recombination with the chromosome at the site of nucleic acid-guided nuclease-mediated double strand break (e.g. FIG. 1B).
A cassette can encode machinery (e.g. targetable nuclease, guide nucleic acid, editing cassette, and/or recorder cassette as disclosed herein) necessary to induce strand breakage as well as designed repair that can be selectively enriched and/or tracked in cells. A cell can be any cell such as eukaryotic cell, archaeal cell, prokaryotic cell, or microorganisms such as E. coli (e.g. FIG. 2A-2D).
A cassette can comprise an editing cassette. A cassette can comprise a recorder cassette. A cassette can comprise a guide nucleic acid and an editing cassette. A cassette can comprise a guide nucleic acid and a recorder cassette. A cassette can comprise a guide nucleic acid, an editing cassette, and a recorder cassette. A cassette can comprise two guide nucleic acids, an editing cassette, and a recorder cassette. A cassette can comprise more than two guide nucleic acids, one or more editing cassettes, and one or more recorder cassettes. These elements of a cassette can be linked covalently. These elements of a cassette can be contiguous. These elements of a cassette can be contiguous.
A cassette can comprise an editing sequence. A cassette can comprise a recorder sequence. A cassette can comprise a guide nucleic acid and an editing sequence. A cassette can comprise a guide nucleic acid and a recorder sequence. A cassette can comprise a guide nucleic acid, an editing sequence, and a recorder sequence. A cassette can comprise two guide nucleic acids, an editing sequence, and a recorder sequence. A cassette can comprise more than two guide nucleic acids, one or more editing sequences, and one or more recorder sequences. These elements of a cassette can be linked covalently. These elements of a cassette can be contiguous. These elements of a cassette can be contiguous.
Single genome edits can be tracked using sequencing technologies, e.g. short read sequencing technologies (e.g. FIG. 1C), long read sequencing technologies, or any other sequencing technologies known in the art.
In some embodiments, upon transformation, each editing cassette generates the designed genetic modification within the transformed cell. In some examples, the editng cassette can act in trans as a barcode of the genetic mutation introduced by the editing cassette and can enable the tracking of this mutation frequency in a complex population over time and across many different growth conditions (e.g. FIG. 2A-2D and FIG. 1C).
In some examples, a recording cassette inserts the designed trackable sequence, such as a marker or barcode sequence, within the transformed cell. In some examples, the recorder cassette can act in cis as a barcode of the chromosomal mutation and can enable the tracking of this mutation frequency in a complex population over time and across many different growth conditions.
By providing cis and/or trans tracking of designed genomic mutations, the methods provided herein simplify sample preparation and depth of coverage for mapping diversity genome wide, and provide powerful tools for engineering on a genome scale (e.g. FIG. 1C).
A plurality of cassettes can be pooled into a library of cassettes. A library of cassettes can comprise at least 2 cassettes. A library of cassettes can comprise from 5 to a million cassettes. A library of cassettes can comprise at least a million cassettes. It should be understood, that a library of cassettes can comprise any number of cassettes.
A library of cassettes can comprise cassettes that have any combination of common elements and non-common or unique elements as compared to the other cassettes within the pool. For example, a library of cassettes can comprise common priming sites or common homology arms while also containing non-common or unique barcodes. Common elements can be shared by a plurality, majority, or all of the cassettes within a library of cassettes. Non-common elements can be shared by a plurality, minority, or sub-population of cassettes within the library of cassettes. Unique elements can be shared by a one, a few, or a sub-population of cassettes within the library of cassettes, such that it is able to identify or distinguish the one, few, or sub-population of cassettes from the other cassettes within the library of cassettes. Such combinations of common and non-common are advantageous for multiplexing techniques as disclosed herein.
Cassettes disclosed herein can generate the designed genetic modification or insert the designed marker or barcode sequence with high efficiency within a transformed cell. In many examples, the efficiency is greater than 50%. In some examples the efficiency is 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 99%, or 100% (e.g., FIGS. 32A, 32B, and 33 ).
In some examples, transformation, editing, and/or recording efficiency can be increased by modulating the expression of one or more components disclosed herein, such as a nucleic acid-guided nuclease. Methods for modulating components are disclosed herein and are known in the art. Such methods can include expressing a component, such as a nucleic acid-guided nuclease or CRISPR enzyme of a subject system on a low or high copy plasmid, depending on the experimental design.
Disclosed herein are methods and compositions for generating cassettes. A cassettes can comprise a cassettes as disclosed herein. For example, a cassette can comprise any combination of an editing cassette and/or recorder cassette disclosed herein. Such a cassette can be comprised on a larger polynucleic acid molecule. Such a larger polynucleic acid molecule can be linear or circular, such as a plasmid or viral vector.
An editing cassette can comprise a mutation relative to a target nucleic acid sequence. The editing cassette can comprise sequence homologous to the target sequence flanking the desired mutation or editing sequence. The editing cassette can comprise a region which recognizes, or hybridizes to, a target sequence of a nucleic acid in a cell or population of cells, is homologous to the target sequence of the nucleic acid of the cell and includes a mutation, or a desired mutation, of at least one nucleotide relative to the target sequence.
An editing cassette can comprise a first editing sequence comprising a first mutation relative to a target sequence. A first mutation can comprise a mutation such as an insertion, deletion, or substitution of at least one nucleotide compared to the non-editing target sequence. The mutation can be incorporated into a coding region or non-coding region.
An editing cassette can comprise a second editing sequence comprising a second mutation relative to a target sequence. The second mutation can be designed to mutate or otherwise silence a PAM sequence such that a corresponding nucleic acid guided nuclease or CRISPR nuclease is no longer able to cleave the target sequence. In such cases, this mutation or silencing of a PAM can serve as a method for selecting transformants in which the first editing sequence has been incorporated.
In some examples, an editing cassette comprises at least two mutations, wherein one mutation is a PAM mutation. In some examples, the PAM mutation can be in a second editing cassette. Such a second editing cassette can be covalently linked and can be continuous or non-contiguous to the other elements in the cassette.
An editing cassette can comprise a guide nucleic acid, such as a gRNA encoding gene, optionally operably linked to a promoter. The guide nucleic acid can be designed to hybridize with the targeted nucleic acid sequence in which the editing sequence will be incorporated.
A recording cassette can comprise a recording sequence. A recorder sequence can comprise a barcoding sequence, or other screenable or selectable marker or fragment thereof. The recording sequence can be comprised within a recorder cassette. Recorder cassettes can comprise regions homologous to an insertion site within a target nucleic acid sequence such that the recording sequence is incorporated by homologous recombination or homology-driven repair systems. The site of incorporation of the recording cassette can be comprised on the same DNA molecule as the target nucleic acid to be edited by an editing cassette. The recorder sequence can comprise a barcode, unique DNA sequence, and/or a complete copy or fragment of a selectable or screenable element or marker.
A recorder cassette can comprise a mutation relative to the target sequence. The mutation can be designed to mutate or otherwise silence a PAM sequence such that a corresponding nucleic acid guided nuclease or CRISPR nuclease is no longer able to cleave the target sequence. In such cases, this mutation or silencing of a PAM site can serve as a method for selecting transformants in which the first recording sequence has been incorporated. A recorder cassette can comprise a PAM mutation. The PAM mutation can be designed to mutate or otherwise silence a PAM site such that a corresponding CRISPR nuclease is no longer able to cleave the target sequence. In such cases, this mutation or silencing of a PAM site can serve as a method for selecting transformants in which the recorder sequence has been incorporated.
A recorder cassette can comprise a guide nucleic acid, such as a gene encoding a gRNA. A promoter can be operably linked to a nucleic acid sequence encoding a guide nucleic acid capable of targeting a nucleic acid-guided nuclease to the desired target sequence. A guide nucleic acid can target a unique site within the target site. In some cases, the guide nucleic acid targets a unique landing site that was incorporated in a prior round of engineering. In some cases, the guide nucleic acid targets a unique landing site that was incorporated by a recorder cassette in a prior round of engineering.
A recorder cassette can comprise a barcode. A barcode can be a unique barcode or relatively unique such that the corresponding mutation can be identified based on the barcode. In some examples, the barcode is a non-naturally occurring sequence that is not found in nature. In most examples, the combination of the desired mutation and the barcode within the editing cassette is non-naturally occurring and not found in nature. A barcode can be any number of nucleotides in length. A barcode can be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, or more than 30 nucleotides in length. In some cases, the barcode is more than 30 nucleotides in length. A barcode can be generated by degenerate oligonucleotide synthesis. A barcode can be rationally designed or user-specified.
A recorder cassette can comprise a landing site. A landing site can serve as a target site for a recorder cassette for a successive engineering round. A landing site can comprise a PAM. A landing site can be a unique sequence. A landing site can be at least about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 35, 40, 45, 50 nucleotides in length. In some cases, the landing site is greater than 50 nucleotides in length.
A recorder cassette can comprise a selectable or screenable marker, or a regulatory sequence or mutation that turns a selectable or screenable marker on or off. In such cases, the turning on or off of a selectable marker can be used of selection or counter-selection, respectively, of iterative rounds of engineering. An example regulatory sequence includes a ribosome-binding site (RBS), though other such regulatory sequences are envisioned. Mutations that turn a selectable or screenable marker on can include any possible start codon that is recognized by the host transcription machinery. A mutation that turns off a selectable or screenable marker includes a mutation that deletes a start codon or one that inserts a premature stop codon or a reading frame shift mutation.
A recorder cassette can comprise one or more of a guide nucleic acid targeting a target site into which the recorder sequence is to be incorporated, a PAM mutation to silence a PAM used by the guide RNA, a barcode corresponding to an editing cassette, a unique site to serve as a landing site for a recorder cassette of a subsequent rounds of engineering, a regulatory sequence or mutation that turns a screenable or selectable marker on or off, these one or more elements being flanked by homology arms that are designed to promote recombination of these one or more elements into the cleaved target site that is targeted by the guide RNA.
A recorder cassette can comprise a first homology arm, a PAM mutation, a barcode, a unique landing site, a regulatory sequence or mutation for a screenable or selectable marker, a second homology arm, and guide RNA. The first homology arm can be an upstream homology arm. The second homology arm can be a downstream homology arm. The homology arms can be homologous to sequences flanking a cleavage site that is targeted by the guide RNA.
A cassette can comprise two guide nucleic acids designed to target two distinct target nucleic acid sequences. In any case, the guide nucleic acid can comprise a single gRNA or chimeric gRNA consisting of a crRNA and trRNA sequences, or alternatively, the gRNA can comprise separated crRNA and trRNAs, or a guide nucleic acid can comprise a crRNA. In other examples, guide nucleic acid can be introduced simultaneously with a trackable polynucleic acid or plasmid comprising an editing cassette and/or recorder cassette. In these cases, the guide nucleic acid can be encoded on a separate plasmid or be delivered in RNA form via delivery methods well known in the art.
A cassette can comprise a gene encoding a nucleic acid-guided nuclease, such as a CRISPR nuclease, functional with the chosen guide nucleic acid. A nucleic acid-guided nuclease or CRISPR nuclease gene can be provided on a separate plasmid. A nucleic acid-guided nuclease or CRISPR nuclease can be provided on the genome or episomal plasmid of a host organism to which a trackable polynucleic acid or plasmid will be introduced. In any of these examples, the nucleic acid-guided nuclease or CRISPR nuclease gene can be operably linked to a constitutive or inducible promotor. Examples of suitable constitutive and inducible promoters are well known in the art. A nucleic acid-guided nuclease or CRISPR nuclease can be provided as mRNA or polypeptide using delivery systems well known in the art. Such mRNA or polypeptide delivery systems can include, but are not limited to, nanoparticles, viral vectors, or other cell-permeable technologies.
A cassette can comprise a selectable or screenable marker, for example, such as that comprised within a recorder cassette. For example, the recorder cassette can comprise a barcode, such as trackable nucleic acid sequence which can be uniquely correlated with a genetic mutation of the corresponding editing cassette, or otherwise identifiably correlated with such a genetic mutation such that sequencing the barcode will allow identification of the corresponding genetic mutation introduced by the editing cassette. In other examples, recorder cassette can comprise a complete copy of or a fragment of a gene encoding an antibiotic resistance gene, auxotrophic marker, fluorescent protein, or other known selectable or screenable markers.

Trackable Plasmid Libraries

A trackable library can comprise a plurality of cassettes as disclosed herein. A trackable library can comprise a plurality of trackable polynucleic acids or plasmids comprising a cassette as disclosed herein. A cassette, polynucleotide, or plasmid comprising a recorder sequence or recorder cassette as disclosed herein can be referred to as a trackable cassette, polynucleotide, or plasmid. A cassette, polynucleotide, or plasmid comprising an editing sequence or editing cassette as disclosed herein can be referred to as a trackable cassette, polynucleotide, or plasmid.
In some cases, within the trackable library are distinct editing cassette and recorder cassette combinations that are sequenced to determine which editing sequence corresponds with a given marker or barcode sequence comprised within the recorder cassette. Therefore, when the editing and recorder sequences are incorporated into a target sequence, you can determine the edit that was incorporated by sequencing the recorder sequence. Sequence the recorder sequence or barcode can significantly cut down on sequencing time and cost.
Library size can depend on the experiment design. For example, if the aim is to edit each amino acid within a protein of interest, then the library size can depend on the number (N) of amino acids in a protein of interest, with a full saturation library (all 20 amino acids at each position or non-naturally occurring amino acids) scaling as 19 (or more)×N and an alanine-mapping library scaling as 1×N. Thus, screening of even very large proteins of more than 1,000 amino acids can be tractable given current multiplex oligo synthesis capabilities (e.g. 120,000 oligos). In addition to or as an alternative to activity screens, more general properties with developed high-throughput screens and selections can be efficiently tested using the libraries disclosed herein. It should be readily understood that libraries can be designed to mutate any number of amino acids within a target protein, including 1, at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, etc. up to the total number of amino acids within a target protein. Additionally, select amino acids can be targeted, such as catalytically active amino acids, or those involved in protein-protein interactions. Each amino acid that is targeted for mutation can be mutated into any number of alternate amino acids, such as any other natural or non-naturally occurring amino acid or amino acid analog. In some examples, all targeted amino acids are mutated to the same amino acid, such as alanine. In other cases, the targeted amino acids are independently mutated to any other amino acid in any combination or permutation.
Trackable libraries can comprise trackable mutations in individual residues or sequences of interest. Trackable libraries can be generated using custom-synthesized oligonucleotide arrays. Trackable plasmids can be generated using any cloning or assembly methods known in the art. For example, CREATE-Recorder plasmids can be generated by chemical synthesis, Gibson assembly, SLIC, CPEC, PCA, ligation-free cloning, other in vitro oligo assembly techniques, traditional ligation-based cloning, or any combination thereof.
Recorder sequences, such as barcodes, can be designed in silico via standard code with a degenerate mutation at the target codon. The degenerate mutation can comprise 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, or more than 30 nucleic acid residues. In some examples, the degenerate mutations can comprise 15 nucleic acid residues (N15).
Homology arms can be added to a recorder sequence and/or editing sequence to allow incorporation of the recorder and/or editing sequence into the desired location via homologous recombination or homology-driven repair. Homology arms can be added by synthesis, in vitro assembly, PCR, or other known methods in the art. For example, homology arms can be assembled via overlapping oligo extension, Gibson assembly, or any other method disclosed herein. A homology arm can be added to both ends of a recorder and/or editing sequence, thereby flanking the sequence with two distinct homology arms, for example, a 5′ homology arm and a 3′ homology arm.
The same 5′ and 3′ homology arms can be added to a plurality of distinct recorder sequences, thereby generating a library of unique recorder sequences that each have the same spacer target or targeted insertion site. The same 5′ and 3′ homology arms can be added to a plurality of distinct editing sequences, thereby generating a library of unique editing sequences that each have the same spacer target or targeted insertion site. In alternative examples, different or a variety of 5′ or 3′ homology arms can be added to a plurality of recorder sequences or editing sequences.
A recorder sequence library comprising flanking homology arms can be cloned into a vector backbone. In some examples, the recorder sequence and homology arms are cloned into a recorder cassette. Recorder cassettes can, in some cases, further comprise a nucleic acid sequence encoding a guide nucleic acid or gRNA engineered to target the desired site of recorder sequence insertion. In many cases, the nucleic acid sequences flanking the CRISPR/Cas-mediated cleavage site are homologous or substantially homologous to the homology arms comprised within the recorder cassette.
An editing sequence library comprising flanking homology arms can be cloned into a vector backbone. In some examples, the editing sequence and homology arms are cloned into an editing cassette. Editing cassettes can, in some cases, further comprise a nucleic acid sequence encoding a guide nucleic acid or gRNA engineered to target the desired site of editing sequence insertion. In many cases, the nucleic acid sequences flanking the CRISPR/Cas-mediated cleavage site are homologous or substantially homologous to the homology arms comprised within the editing cassette.
Gene-wide or genome-wide editing libraries can be subcloned into a vector backbone. In some cases, the vector backbone comprises a recorder cassette as disclosed herein. The editing sequence library can be inserted or assembled into a second site to generate competent trackable plasmids that can embed the recording barcode at a fixed locus while integrating the editing libraries at a wide variety of user defined sites.
A recorder sequence and/or cassette can be assembled or inserted into a vector backbone first, followed by insertion of an editing sequence and/or cassette. In other cases, an editing sequence and/or cassette can be inserted or assembled into a vector backbone first, followed by insertion of a recorder sequence and/or cassette. In other cases, a recorder sequence and/or cassette and an editing sequence and/or cassette are simultaneous inserted or assembled into a vector. In other cases, a recorder sequence and/or cassette and an editing sequence and/or cassette are comprised on the same cassette prior to simultaneous insertion or assembly into a vector. In other cases, a recorder sequence and/or cassette and an editing sequence and/or cassette are linked prior to simultaneous insertion or assembly into a vector. In other cases, a recorder sequence and/or cassette and an editing sequence and/or cassette are covalently linked prior to simultaneous insertion or assembly into a vector. In any of these cases, trackable plasmids or plasmid libraries can be generated.
A cassette or nucleic acid molecule can be synthesized which comprises one or more elements disclosed herein. For example, a nucleic acid molecule can be synthesized that comprises an editing cassette and a guide nucleic acid. A nucleic acid molecule can be synthesized that comprises an editing cassette and a recorder cassette. A nucleic acid molecule can be synthesized that comprises an editing cassette, a guide nucleic acid, and a recorder cassette. A nucleic acid molecule can be synthesized that comprises an editing cassette, a recorder cassette, and two guide nucleic acids. A nucleic acid molecule can be synthesized that comprises a recorder cassette and a guide nucleic acid. A nucleic acid molecule can be synthesized that comprises a recorder cassette. A nucleic acid molecule can be synthesized that comprises an editing cassette. In any of these cases, the guide nucleic acid can optionally be operably linked to a promoter. In any of these cases, the nucleic acid molecule can further include one or more barcodes.
Synthesized cassettes or synthesized nucleic acid molecules can be synthesized using any oligonucleotide synthesis method known in the art. For example, cassettes can be synthesized by array based oligonucleotide synthesis. In such examples, following synthesis of the oligonucleotides, the oligonucleotides can be cleaved from the array. Cleavage of oligonucleotides from an array can create a pool of oligonucleotides.
Software and automation methods can be used for multiplex synthesis and generation. For example, software and automation can be used to create 10, 10², 10³, 10⁴, 10⁵, 10⁶, or more cassettes, such as trackable cassettes. An automation method can generate trackable plasmids in rapid fashion. Trackable cassettes can be processed through a workflow with minimal steps to produce precisely defined genome-wide libraries.
Cassette libraries, such as trackable cassette libraries, can be generated which comprise two or more nucleic acid molecules or plasmids comprising any combination disclosed herein of recorder sequence, editing sequence, guide nucleic acid, and optional barcode, including combinations of one or more of any of the previously mentioned elements. For example, such a library can comprise at least 2, 3, 4, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500, 5000, 5500, 6000, 6500, 7000, 7500, 8000, 8500, 9000, 9500, 10⁴, 10⁵, 10⁶, 10⁷, 10⁸, 10⁹, 10¹⁰, or more nucleic acid molecules or plasmids of the present disclosure. It should be understood that such a library can include any number of nucleic acid molecules or plasmids, even if the specific number is not explicit listed above.
Cassettes or cassette libraries can be sequenced in order to determine the recorder sequence and editing sequence pair that is comprised on each cassette. In other cases, a known recorder sequence is paired with a known editing sequence during the library generation process. Other methods of determining the association between a recorder sequence and editing sequence comprised on a common nucleic acid molecule or plasmid are envisioned such that the editing sequence can be identified by identification or sequencing of the recorder sequence.
Methods and compositions for tracking edited episomal libraries that are shuttled between E. coli and other organisms/cell lines are provided herein. The libraries can be comprised on plasmids, Bacterial artificial chromosomes (BACs), Yeast artificial chromosomes (YACs), synthetic chromosomes, or viral or phage genomes. These methods and compositions can be used to generate portable barcoded libraries in host organisms, such as E. coli. Library generation in such organisms can offer the advantage of established techniques for performing homologous recombination. Barcoded plasmid libraries can be deep-sequenced at one site to track mutational diversity targeted across the remaining portions of the plasmid allowing dramatic improvements in the depth of library coverage (e.g. FIG. 3A).

Trackable Engineering Methods

An example of trackable engineering workflow is depicted in FIG. 3A. Each plasmid can encode a recorder cassette designed to edit a site in the target DNA (e.g. FIG. 3A, black cassette). Sites to be targeted can be functionally neutral sites, or they can be a screenable or selectable marker gene. The homology arm (HA) of the recorder cassette can contain a recorder sequence (e.g., FIG. 3B) that is inserted into the recording site during recombineering. Recombineering can comprise DNA cleavage, such as nucleic acid-guided nuclease-mediated DNA cleavage, and repair via homologous recombination. The recorder sequence can comprise a barcode, unique DNA sequence, or a complete copy or fragment of a screenable or selectable marker. In some examples, the recorder sequence is 15 nucleotides. The recorder sequence can comprise less than 10, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 88, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, or more than 200 nucleotides.
Through a multiplexed cloning approach, the recorder cassette can be covalently coupled to at least one editing cassette in a plasmid (e.g., FIG. 3A, green cassette) to generate trackable plasmid libraries that have a unique recorder and editing cassette combination. This trackable library can be sequenced to generate the recorder/edit mapping and used to track editing libraries across large segments of the target DNA (e.g., FIG. 3C). Recorder and editing sequences can be comprised on the same polynucleotide, in which case they are both incorporated into the target nucleic acid sequence, such as a genome or plasmid, by the same recombination event. In other examples, the recorder and editing sequences can be comprised on separate cassettes within the same trackable plasmid, in which case the recorder and editing sequences are incorporated into the target nucleic acid sequence by separate recombination events, either simultaneously or sequentially.
Methods are provided herein for combining multiplex oligonucleotide synthesis with recombineering, to create libraries of specifically designed and trackable mutations. Screens and/or selections followed by high-throughput sequencing and/or barcode microarray methods can allow for rapid mapping of mutations leading to a phenotype of interest.
Methods and compositions disclosed herein can be used to simultaneously engineer and track engineering events in a target nucleic acid sequence.
Trackable plasmids can be generated using in vitro assembly or cloning techniques. For example, the CREATE-Recorder plasmids can be generated using chemical synthesis, Gibson assembly, SLIC, CPEC, PCA, ligation-free cloning, other in vitro oligo assembly techniques, traditional ligation-based cloning, or any combination thereof.
Trackable plasmids can comprise at least one recording sequence, such as a barcode, and at least one editing sequence. In most cases, the recording sequence is used to record and track engineering events. Each editing sequence can be used to incorporate a desired edit into a target nucleic acid sequence. The desired edit can include insertion, deletion, substitution, or alteration of the target nucleic acid sequence. In some examples, the one or more recording sequence and editing sequences are comprised on a single cassette comprised within the trackable plasmid such that they are incorporated into the target nucleic acid sequence by the same engineering event. In other examples, the recording and editing sequences are comprised on separate cassettes within the trackable plasmid such that they are each incorporated into the target nucleic acid by distinct engineering events. In some examples, the trackable plasmid comprises two or more editing sequences. For example, one editing sequence can be used to alter or silence a PAM sequence while a second editing sequence can be used to incorporate a mutation into a distinct sequence.
Recorder sequences can be inserted into a site separated from the editing sequence insertion site. The inserted recorder sequence can be separated from the editing sequence by 1 bp or any number of base pairs. For example, the separation distance can be about 1 bp, 10 bp, 50 bp, 100 bp, 500 bp, lkp, 2 kb, 5 kb, 10 kb, or greater. The separation distance can be any discrete integer of base pairs. It should be readily understood that there the limit of the number of base pairs separating the two insertion sites can be limited by the size of the genome, chromosome, or polynucleotide into which the insertions are being made. In some examples, the maximum distance of separation depends on the size of the target nucleic acid or genome.
Recorder sequences can be inserted adjacent to editing sequences, or within proximity to the editing sequence. For example, the recorder sequence can be inserted outside of the open reading frame within which the editing sequence is inserted. Recorder sequence can be inserted into an untranslated region adjacent to an open reading frame within which an editing sequence has been inserted. The recorder sequence can be inserted into a functionally neutral or non-functional site. The recorder sequence can be inserted into a screenable or selectable marker gene.
In some examples, the target nucleic acid sequence is comprised within a genome, artificial chromosome, synthetic chromosome, or episomal plasmid. In various examples, the target nucleic acid sequence can be in vitro or in vivo. When the target nucleic acid sequence is in vivo, the CREATE-Recorder plasmid can be introduced into the host organisms by transformation, transfection, conjugation, biolistics, nanoparticles, cell-permeable technologies, or other known methods for DNA delivery, or any combination thereof. In such examples, the host organism can be a eukaryote, prokaryote, bacterium, archaea, yeast, or other fungi.
The engineering event can comprise recombineering, non-homologous end joining, homologous recombination, or homology-driven repair. In some examples, the engineering event is performed in vitro or in vivo.
The methods described herein can be carried out in any type of cell in which a nucleic acid-guided nuclease system can function (e.g., target and cleave DNA), including prokaryotic and eukaryotic cells or in vitro. In some embodiments the cell is a bacterial cell, such as Escherichia spp. (e.g., E. coli). In other embodiments, the cell is a fungal cell, such as a yeast cell, e.g., Saccharomyces spp. In other embodiments, the cell is an algal cell, a plant cell, an insect cell, or a mammalian cell, including a human cell.
In some examples, a cell is a recombinant organism. For example, the cell can comprise a non-native nucleic acid-guided nuclease system. Additionally or alternatively, the cell can comprise recombination system machinery. Such recombination systems can include lambda red recombination system, Cre/Lox, attB/attP, or other integrase systems. Where appropriate, the trackable plasmid can have the complementary components or machinery required for the selected recombination system to work correctly and efficiently.
A method for genome editing can comprise: (a) introducing a vector that encodes at least one editing cassette and at least one guide nucleic acid into a first population of cells, thereby producing a second population of cells comprising the vector; (b) maintaining the second population of cells under conditions in which a nucleic acid-guided nuclease is expressed or maintained, wherein the nucleic acid-guided nuclease is encoded on the vector, a second vector, on the genome of cells of the second population of cells, or otherwise introduced into the cell, resulting in DNA cleavage and incorporation of the editing cassette; (c) obtaining viable cells. Such a method can optionally further comprise (d) sequencing the target DNA molecule in at least one cell of the second population of cells to identify the mutation of at least one codon.
A method for genome editing can comprise: (a) introducing a vector that encodes at least one editing cassette comprising a PAM mutation as disclosed herein and at least one guide nucleic acid into a first population of cells, thereby producing a second population of cells comprising the vector; (b) maintaining the second population of cells under conditions in which nucleic acid-guided nuclease is expressed or maintained, wherein the nucleic acid-guided nuclease is encoded on the vector, a second vector, on the genome of cells of the second population of cells, or otherwise introduced into the cell, resulting in DNA cleavage, incorporation of the editing cassette, and death of cells of the second population of cells that do not comprise the PAM mutation, whereas cells of the second population of cells that comprise the PAM mutation are viable; (c) obtaining viable cells. Such a method can optionally further comprise (d) sequencing the target DNA in at least one cell of the second population of cells to identify the mutation of at least one codon.
Method for trackable genome editing can comprise: (a) introducing a vector that encodes at least one editing cassette, at least one recorder cassette, and at least two gRNA into a first population of cells, thereby producing a second population of cells comprising the vector; (b) maintaining the second population of cells under conditions in which a nucleic acid-guided nuclease is expressed or maintained, wherein the nucleic acid-guided nuclease is encoded on the vector, a second vector, on the genome of cells of the second population of cells, or otherwise introduced into the cell, resulting in DNA cleavage and incorporation of the editing and recorder cassettes; (c) obtaining viable cells. Such a method can optionally further comprise (d) sequencing the recorder sequence of the target DNA molecule in at least one cell of the second population of cells to identify the mutation of at least one codon.
In some examples where the trackable plasmid comprises an editing cassette designed to silence a PAM site, a method for trackable genome editing can comprise: (a) introducing a vector that encodes at least one editing cassette, a recorder cassette, and at least two gRNA into a first population of cells, thereby producing a second population of cells comprising the vector; (b) maintaining the second population of cells under conditions in which a nucleic acid-guided nuclease is expressed or maintained, wherein the nucleic acid-guided nuclease is encoded on the vector, a second vector, on the genome of cells of the second population of cells, or otherwise introduced into the cell, resulting in DNA cleavage, incorporation of the editing cassette and recorder cassette, and death of cells of the second population of cells that do not comprise the PAM mutation, whereas cells of the second population of cells that comprise the PAM mutation are viable; and (c) obtaining viable cells. Such a method can optionally further comprise (d) sequencing the recorder sequence of the target DNA in at least one cell of the second population of cells to identify the mutation of at least one codon. Such methods can also further comprise a recorder cassette comprising a second PAM mutation, such that both PAMs must be silences by the editing cassette PAM mutation and recorder cassette PAM mutation in order to escape cell death.
In some examples transformation efficiency is determined by using a non-targeting guide nucleic acid control, which allows for validation of the recombineering procedure and CFU/ng calculations. In some cases, absolute efficient is obtained by counting the total number of colonies on each transformation plate, for example, by counting both red and white colonies from a galK control. In some examples, relative efficiency is calculated by the total number of successful transformants (for example, white colonies) out of all colonies from a control (for example, galK control).
The methods of the disclosure can provide, for example, greater than 1000× improvements in the efficiency, scale, cost of generating a combinatorial library, and/or precision of such library generation.
The methods of the disclosure can provide, for example, greater than: 10×, 50×, 100×, 200×, 300×, 400×, 500×, 600×, 700×, 800×, 900×, 1000×, 1100×, 1200×, 1300×, 1400×, 1500×, 1600×, 1700×, 1800×, 1900×, 2000×, or greater improvements in the efficiency of generating genomic or combinatorial libraries.
The methods of the disclosure can provide, for example, greater than: 10×, 50×, 100×, 200×, 300×, 400×, 500×, 600×, 700×, 800×, 900×, 1000×, 1100×, 1200×, 1300×, 1400×, 1500×, 1600×, 1700×, 1800×, 1900×, 2000×, or greater improvements in the scale of generating genomic or combinatorial libraries.
The methods of the disclosure can provide, for example, greater than: 10×, 50×, 100×, 200×, 300×, 400×, 500×, 600×, 700×, 800×, 900×, 1000×, 1100×, 1200×, 1300×, 1400×, 1500×, 1600×, 1700×, 1800×, 1900×, 2000×, or greater decrease in the cost of generating genomic or combinatorial libraries.
The methods of the disclosure can provide, for example, greater than: 10×, 50×, 100×, 200×, 300×, 400×, 500×, 600×, 700×, 800×, 900×, 1000×, 1100×, 1200×, 1300×, 1400×, 1500×, 1600×, 1700×, 1800×, 1900×, 2000×, or greater improvements in the precision of genomic or combinatorial library generation.

Recursive Tracking for Combinatorial Engineering

Disclosed herein are methods and compositions for iterative rounds of engineering. Disclosed herein are recursive engineering strategies that allow implementation of trackable engineering at the single cell level through several serial engineering cycles (e.g., FIG. 3D or FIG. 6 ). These disclosed methods and compositions can enable search-based technologies that can effectively construct and explore complex genotypic space. The terms recursive and iterative can be used interchangeably.
Combinatorial engineering methods can comprise multiple rounds of engineering. Methods disclosed herein can comprise 2 or more rounds of engineering. For example, a method can comprise 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 20, 25, 30, or more than 30 rounds of engineering.
In some examples, during each round of engineering a new recorder sequence, such as a barcode, is incorporated at the same or nearby locus in a target site (e.g., FIG. 3D, green bars or FIG. 6 , black bars) such that following multiple engineering cycles to construct combinatorial diversity throughout the genome (e.g., FIG. 3E, green bars or FIG. 6 , grey bars) a PCR, or similar reaction, of the recording locus can be used to reconstruct each combinatorial genotype or to confirm that the engineered edit from each round has been incorporated into the target site.
Disclosed herein are methods for selecting for successive rounds of engineering. Selection can occur by a PAM mutation incorporated by an editing cassette. Selection can occur by a PAM mutation incorporated by a recorder cassette. Selection can occur using a screenable, selectable, or counter-selectable marker. Selection can occur by targeting a site for editing or recording that was incorporated by a prior round of engineering, thereby selecting for variants that successfully incorporated edits and recorder sequences from both rounds or all prior rounds of engineering.
Quantitation of these genotypes can be used for understanding combinatorial mutational effects on large populations and investigation of important biological phenomena such as epistasis.
Serial editing and combinatorial tracking can be implemented using recursive vector systems as disclosed herein. These recursive vector systems can be used to move rapidly through the transformation procedure (e.g., FIG. 7A). In some examples, these systems consist of two or more plasmids containing orthogonal replication origins, antibiotic markers, and gRNAs. The gRNA in each vector can be designed to target one of the other resistance markers for destruction by nucleic acid-guided nuclease-mediated cleavage. These systems can be used, in some examples, to perform transformations in which the antibiotic selection pressure is switched to remove the previous plasmid and drive enrichment of the next round of engineered genomes. Two or more passages through the transformation loop can be performed, or in other words, multiple rounds of engineering can be performed. Introducing the requisite recording cassettes and editing cassettes into recursive vectors as disclosed herein can be used for simultaneous genome editing and plasmid curing in each transformation step with high efficiencies.
In some examples, the recursive vector system disclosed herein comprises 2, 3, 4, 5, 6, 7, 8, 9, 10, or more than 10 unique plasmids. In some examples, the recursive vector system can use a particular plasmid more than once as long as a distinct plasmid is used in the previous round and in the subsequent round.
Recursive methods and compositions disclosed herein can be used to restore function to a selectable or screenable element in a targeted genome or plasmid. The selectable or screenable element can include an antibiotic resistance gene, a fluorescent gene, a unique DNA sequence or watermark, or other known reporter, screenable, or selectable gene. In some examples, each successive round of engineering can incorporate a fragment of the selectable or screenable element, such that at the end of the engineering rounds, the entire selectable or screenable element has been incorporated into the target genome or plasmid. In such examples, only those genome or plasmids, which have successfully incorporated all of the fragments, and therefore all of the desired corresponding mutations, can be selected or screened for. In this way, the selected or screened cells will be enriched for those that have incorporated the edits from each and every iterative round of engineering.
Recursive methods can be used to switch a selectable or screenable marker between an on and an off position, or between an off and an on position, with each successive round of engineering. Using such a method allows conservation of available selectable or screenable markers by requiring, for example, the use of only one screenable or selectable marker. Furthermore, short regulatory sequence or start codon or non-start codons can be used to turn the screenable or selectable marker on and off. Such short sequences can easily fit within a cassette or polynucleotide, such as a synthesized cassette.
One or more rounds of engineering can be performed using the methods and compositions disclosed herein. In some examples, each round of engineering is used to incorporate an edit unique from that of previous rounds. Each round of engineering can incorporate a unique recording sequence. Each round of engineering can result in removal or curing of the CREATE plasmid used in the previous round of engineering. In some examples, successful incorporation of the recording sequence of each round of engineering results in a complete and functional screenable or selectable marker or unique sequence combination.
Unique recorder cassettes comprising recording sequences such as barcodes or screenable or selectable markers can be inserted with each round of engineering, thereby generating a recorder sequence that is indicative of the combination of edits or engineering steps performed. Successive recording sequences can be inserted adjacent to one another. Successive recording sequences can be inserted within proximity to one another. Successive sequences can be inserted at a distance from one another.
Successive sequences can be inserted at a distance from one another. For example, successive recorder sequences can be inserted and separated by 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, or greater than 100 bp. In some examples, successive recorder sequences are separated by about 10, 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000, 1100, 1200, 1300, 1400, 1500, or greater than 1500 bp.
Successive recorder sequences can be separated by any desired number of base pairs and can be dependent and limited on the number of successive recorder sequences to be inserted, the size of the target nucleic acid or target genomes, and/or the design of the desired final recorder sequence. For example, if the compiled recorder sequence is a functional screenable or selectable marker, than the successive recording sequences can be inserted within proximity and within the same reading frame from one another. If the compiled recorder sequence is a unique set of barcodes to be identified by sequencing and have no coding sequence element, then the successive recorder sequences can be inserted with any desired number of base pairs separating them. In these cases, the separation distance can be dependent on the sequencing technology to be used and the read length limit.
In some examples, a recorder cassette comprises a landing site to be used as a target site for the recorder cassette of the next round of engineering. By using such a method, successive rounds of recorder cassettes can only be introduced into the target site if the recorder cassette from the previous round was successfully incorporated, thereby providing the target site for the present engineering round (e.g., FIG. 28 ).

Guide Nucleic Acid

A guide nucleic acid can complex with a compatible nucleic acid-guided nuclease and can hybridize with a target sequence, thereby directing the nuclease to the target sequence. A subject nucleic acid-guided nuclease capable of complexing with a guide nucleic acid can be referred to as a nucleic acid-guided nuclease that is compatible with the guide nucleic acid. Likewise, a guide nucleic acid capable of complexing with a nucleic acid-guided nuclease can be referred to as a guide nucleic acid that is compatible with the nucleic acid-guided nucleases.
A guide nucleic acid can be DNA. A guide nucleic acid can be RNA. A guide nucleic acid can comprise both DNA and RNA. A guide nucleic acid can comprise modified of non-naturally occurring nucleotides. In cases where the guide nucleic acid comprises RNA, the RNA guide nucleic acid can be encoded by a DNA sequence on a polynucleotide molecule such as a plasmid, linear construct, or editing cassette as disclosed herein.
A guide nucleic acid can comprise a guide sequence. A guide sequence is a polynucleotide sequence having sufficient complementarity with a target polynucleotide sequence to hybridize with the target sequence and direct sequence-specific binding of a complexed nucleic acid-guided nuclease to the target sequence. The degree of complementarity between a guide sequence and its corresponding target sequence, when optimally aligned using a suitable alignment algorithm, is about or more than about 50%, 60%, 75%, 80%, 85%, 90%, 95%, 97.5%, 99%, or more. Optimal alignment may be determined with the use of any suitable algorithm for aligning sequences. In some embodiments, a guide sequence is about or more than about 5, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 35, 40, 45, 50, 75, or more nucleotides in length. In some embodiments, a guide sequence is less than about 75, 50, 45, 40, 35, 30, 25, 20 nucleotides in length. Preferably the guide sequence is 10-30 nucleotides long. The guide sequence can be 15-20 nucleotides in length. The guide sequence can be 15 nucleotides in length. The guide sequence can be 16 nucleotides in length. The guide sequence can be 17 nucleotides in length. The guide sequence can be 18 nucleotides in length. The guide sequence can be 19 nucleotides in length. The guide sequence can be 20 nucleotides in length.
A guide nucleic acid can comprise a scaffold sequence. In general, a “scaffold sequence” includes any sequence that has sufficient sequence to promote formation of a targetable nuclease complex, wherein the targetable nuclease complex comprises a nucleic acid-guided nuclease and a guide nucleic acid comprising a scaffold sequence and a guide sequence. Sufficient sequence within the scaffold sequence to promote formation of a targetable nuclease complex may include a degree of complementarity along the length of two sequence regions within the scaffold sequence, such as one or two sequence regions involved in forming a secondary structure. In some cases, the one or two sequence regions are comprised or encoded on the same polynucleotide. In some cases, the one or two sequence regions are comprised or encoded on separate polynucleotides. Optimal alignment may be determined by any suitable alignment algorithm, and may further account for secondary structures, such as self-complementarity within either the one or two sequence regions. In some embodiments, the degree of complementarity between the one or two sequence regions along the length of the shorter of the two when optimally aligned is about or more than about 25%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 97.5%, 99%, or higher. In some embodiments, at least one of the two sequence regions is about or more than about 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 40, 50, or more nucleotides in length.
A scaffold sequence of a subject guide nucleic acid can comprise a secondary structure. A secondary structure can comprise a pseudoknot region. In some example, the compatibility of a guide nucleic acid and nucleic acid-guided nuclease is at least partially determined by sequence within or adjacent to a pseudoknot region of the guide RNA. In some cases, binding kinetics of a guide nucleic acid to a nucleic acid-guided nuclease is determined in part by secondary structures within the scaffold sequence. In some cases, binding kinetics of a guide nucleic acid to a nucleic acid-guided nuclease is determined in part by nucleic acid sequence with the scaffold sequence.
In aspects of the invention the terms “guide nucleic acid” refers to a polynucleotide comprising 1) a guide sequence capable of hybridizing to a target sequence and 2) a scaffold sequence capable of interacting with or complexing with an nucleic acid-guided nuclease as described herein.
A guide nucleic acid can be compatible with a nucleic acid-guided nuclease when the two elements can form a functional targetable nuclease complex capable of cleaving a target sequence. Often, a compatible scaffold sequence for a compatible guide nucleic acid can be found by scanning sequences adjacent to a native nucleic acid-guided nuclease loci. In other words, native nucleic acid-guided nucleases can be encoded on a genome within proximity to a corresponding compatible guide nucleic acid or scaffold sequence.
Nucleic acid-guided nucleases can be compatible with guide nucleic acids that are not found within the nucleases endogenous host. Such orthogonal guide nucleic acids can be determined by empirical testing. Orthogonal guide nucleic acids can come from different bacterial species or be synthetic or otherwise engineered to be non-naturally occurring.
Orthogonal guide nucleic acids that are compatible with a common nucleic acid-guided nuclease can comprise one or more common features. Common features can include sequence outside a pseudoknot region. Common features can include a pseudoknot region. Common features can include a primary sequence or secondary structure.
A guide nucleic acid can be engineered to target a desired target sequence by altering the guide sequence such that the guide sequence is complementary to the target sequence, thereby allowing hybridization between the guide sequence and the target sequence. A guide nucleic acid with an engineered guide sequence can be referred to as an engineered guide nucleic acid. Engineered guide nucleic acids are often non-naturally occurring and are not found in nature.

More Methods

Disclosed herein are methods for genome engineering that employ a nuclease, such as a nucleic acid-guided nuclease to perform directed genome evolution/produce changes (deletions, substitutions, additions) in a target sequence, such as DNA or RNA, for example, genomic DNA or episomal DNA. Suitable nucleases can include, for example, RNA-guided nucleases such as Cas9, Cpf1, MAD2, or MAD7, DNA-guided nucleases such as Argonaute, or other nucleases such as zinc-finger nucleases, TALENs, or meganucleases. Nuclease genes can be obtained from any source, such as from a bacterium, archaea, prokaryote, eukaryote, or virus. For example, a Cas9 gene can be obtained from a bacterium harboring the corresponding Type II CRISPR system, such as the bacterium S. pyogenes (SEQ ID NO: 110). The nucleic acid sequence and/or amino acid sequence of the nuclease may be mutated, relative to the sequence of a naturally occurring nuclease. A mutation can be, for example, one or more insertions, deletions, substitutions or any combination of two or three of the foregoing. In some cases, the resulting mutated nuclease can have enhanced or reduced nuclease activity relative to the naturally occurring nuclease. In some cases, the resulting mutated nuclease can have no nuclease activity relative to the naturally occurring nuclease.
Methods for nucleic acid-guided nuclease-mediated genome editing are provided herein. Some disclosed methods can include a two-stage construction process which relies on generation of cassette libraries that incorporate directed mutations from an editing cassettes directly into a genome, episomal nucleic acid molecule, or isolated nucleic acid molecule. In some examples, during the first stage of cassette library construction, rationally designed editing cassettes can be cotransformed into cells with a guide nucleic acid (e.g., guide RNA) that hybridizes to or targets a target DNA sequence. In some examples, the guide nucleic acid is introduced as an RNA molecule, or encoded on a DNA molecule.
Editing cassettes can be designed such that they couple deletion or mutation of a PAM site with the mutation of one or more desired codons or nucleic acid residues in the adjacent nucleic acid sequence. The deleted or mutated PAM site, in some cases, can no longer be recognized by the chosen nucleic acid-guided nuclease. In some examples, at least one PAM or more than one PAM can be deleted or mutated, such as two, three, four, or more PAMs.
Methods disclosed herein can enable generation of an entire cassette library in a single transformation. The cassette library can be retrieved, in some cases, by amplification of the recombinant chromosomes, e.g. by a PCR reaction, using a synthetic feature or priming site from the editing cassettes. In some examples, a second PAM deletion or mutation is simultaneously incorporated. This approach can covalently couple the codon-targeted mutations directly to a PAM deletion.
In some examples, there is a second stage to construction of cassette libraries. During the second stage the PCR amplified cassette libraries carrying the destination PAM deletion/mutation and the targeted mutations, such as a desired mutation of one or more nucleotides, such as one or more nucleotides in one or more codons, can be co-transformed into naive cells. The cells can be eukaryotic cell, archaeal cell, or prokaryotic cells. The cassette libraries can be co-transformed with a guide nucleic acid or plasmid encoding the same to generate a population of cells that express a rationally designed protein library. The libraries can be co-transformed with a guide nucleic acid such as a gRNA, chimeric gRNA, split gRNA, or a crRNA and trRNA set. The cassette library can comprise a plurality of cassettes wherein each cassette comprises an editing cassette and guide nucleic acid. The cassette library can comprise a plurality of cassettes wherein each cassette comprises an editing cassette, recorder cassettes and two guide nucleic acids.
In some targetable nuclease systems, the guide nucleic acid can guide selection of a target sequence. As used herein, a target sequence refers to any locus in vitro or in in vivo, or in the nucleic acid of a cell or population of cells in which a mutation of at least one nucleotide, such as a mutation of at least one nucleotide in at least one codon, is desired. The target sequence can be, for example, a genomic locus, target genomic sequence, or extrachromosomal locus. The guide nucleic acid can be expressed as a DNA molecule, referred to as a guide DNA, or as a RNA molecule, referred to as a guide RNA. A guide nucleic acid can comprise a guide sequence, that is complementary to a region of the target region. A guide nucleic acid can comprise a scaffold sequence that can interact with a compatible nucleic acid-guided nuclease, and can optionally form a secondary structure. A guide nucleic acid can functions to recruit a nucleic acid-guided nuclease to the target site. A guide sequence can be complementary to a region upstream of the target site. A guide sequence can be complementary to at least a portion of the target site. A guide sequence can be completely complementary (100% complementary) to the target site or include one or more mismatches, provided that it is sufficiently complementary to the target site to specifically hybridize/guide and recruit the nuclease. Suitable nucleic acid guided nuclease include, as non-limiting examples, CRISPR nucleases, Cas nucleases, such as Cas9 or Cpf1, MAD2, and MAD7.
In some CRISPR systems, the CRISPR RNA (crRNA or spacer-containing RNA) and trans-activating CRISPR RNA (tracrRNA or trRNA) can guide selection of a target sequence. As used herein, a target sequence refers to any locus in vitro or in in vivo, or in the nucleic acid of a cell or population of cells in which a mutation of at least one nucleotide, such as a mutation of at least one nucleotide in at least one codon, is desired. The target sequence can be, for example, a genomic locus, target genomic sequence, or extrachromosomal locus. The tracrRNA and crRNA can be expressed as a single, chimeric RNA molecule, referred to as a single-guide RNA, guide RNA, or gRNA. The nucleic acid sequence of the gRNA comprises a first nucleic acid sequence, also referred to as a first region, that is complementary to a region of the target region and a second nucleic acid sequence, also referred to a second region, that forms a stem loop structure and functions to recruit a CRISPR nuclease to the target region. The first region of the gRNA can be complementary to a region upstream of the target genomic sequence. The first region of the gRNA can be complementary to at least a portion of the target region. The first region of the gRNA can be completely complementary (100% complementary) to the target genomic sequence or include one or more mismatches, provided that it is sufficiently complementary to the target genomic sequence to specifically hybridize/guide and recruit a CRISPR nuclease, such as Cas9 or Cpf1.
A guide sequence or first region of the gRNA can be at least 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or at least 30 nucleotides in length. The guide sequence or first region of the gRNA can be at least 20 nucleotides in length.
A stem loop structure that can be formed by the scaffold sequence or second nucleic acid sequence of a gRNA can be at least 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 7, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100 nucleotides in length. A stem loop structure can be from 80 to 90 or 82 to 85 nucleotides in length. A scaffold sequence or second region of the gRNA that forms a stem loop structure can be 83 nucleotides in length.
A guide nucleic acid of a cassette that is introduced into a first cell using the methods disclosed herein can be the same as the guide nucleic acid of a second cassette that is introduced into a second cell. More than one guide nucleic acid can be introduced into the population of first cells and/or the population of second cells. The more than one guide nucleic acids can comprise guide sequences that are complementary to more than one target region.
Methods disclosed herein can comprise using oligonucleotides. Such oligonucleotides can be obtained or derived from many sources. For example, an oligonucleotide can be derived from a nucleic acid library that has been diversified by nonhomologous random recombination (NRR); such a library is referred to as an NRR library. An oligonucleotide can be synthesized, for example by array-based synthesis or other known chemical synthesis method. The length of an oligonucleotide can be dependent on the method used in obtaining the oligonucleotide. An oligonucleotide can be approximately 50-200 nucleotides, 75-150 nucleotides, or between 80-120 nucleotides in length. An oligonucleotide can be about 10, 20, 30, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, or more nucleotides in length, including any integer, for example, 51, 52, 53, 54, 201, 202, etc. An oligonucleotide can be about 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1250, 1500, 1750, 2000, or more nucleotides in length, including any integer, for example, 101, 203, 1001, 2001, 2010, etc.
Oligonucleotides and/or other nucleic acid molecules can be combined or assembled to generate a cassette. Such a cassette can comprise (a) a region that is homologous to a target region of the nucleic acid of the cell and includes a desired mutation of at least one nucleotide or one codon relative to the target region, and (b) a protospacer adjacent motif (PAM) mutation. The PAM mutation can be any insertion, deletion or substitution of one or more nucleotides that mutates the sequence of the PAM such that it is no longer recognized by a nucleic acid-guided nuclease system or CRISPR nuclease system. A cell that comprises such a PAM mutation may be said to be “immune” to nuclease-mediated killing. The desired mutation relative to the sequence of the target region can be an insertion, deletion, and/or substitution of one or more nucleotides. In some examples, the insertion, deletion, and/or substitution of one or more nucleotides is in at least one codon of the target region. Alternatively, the cassette can be synthesized in a single synthesis, comprising (a) a region that is homologous to a target region of the nucleic acid of the cell and includes a desired mutation of at least one nucleotide or one codon relative to the target region, (b) a protospacer adjacent motif (PAM) mutation, and optionally (c) a region that is homologous to a second target region of the nucleic acid of the cell and includes a recorder sequence.
The methods disclosed herein can be applied to any target nucleic acid molecule of interest, from any prokaryote including bacteria and archaea, or any eukaryote, including yeast, mammalian, and human genes, or any viral particle. The nucleic acid module can be a non-coding nucleic acid sequence, gene, genome, chromosome, plasmid, episomal nucleic acid molecule, artificial chromosome, synthetic chromosome, or viral nucleic acid.
Methods for assessing recovery efficiency of donor strain libraries are disclosed herein. Recovery efficiency can be verified based on the presence of a PCR product or on changes in amplicon or PCR product sizes or sequence obtained with primers directed at the selected target locus. Primers can be designed to hybridize with endogenous sequences or heterologous sequences contained on the donor nucleic acid molecule. For example, the PCR primer can be designed to hybridize to a heterologous sequence such that PCR will only be possible if the donor nucleic acid is incorporated. Sequencing of PCR products from the recovered libraries indicates the heterologous sequence or synthetic priming site from the dsDNA cassettes or donor sequences can be incorporated with about 90-100% efficiency. In other examples, the efficiency can be about 5%, 10% 20%, 30%, 40%, 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 99% or 100%.
In some cases, the ability to improve final editing efficiencies of the methods disclosed herein can be assessed by carrying out cassette construction in gene deficient strains before transferring to a wild-type donor strain in an effort to prevent loss of mutations during the donor construction phase. Additionally or alternatively, efficiency of the disclosed methods can be assessed by targeting an essential gene. Essential genes can include any gene required for survival or replication of a viral particle, cell, or organism. In some examples, essential genes include dxs, metA, and folA. Essential genes have been effectively targeted using guide nucleic acid design strategies described. Other suitable essential genes are well known in the art.
Provided herein are method of increasing editing efficiencies by modulating the level of a nucleic acid-guided nuclease. This could be done by using copy control plasmids, such as high copy number plasmids or low copy number plasmids. Low copy number plasmids could be plasmids that can have about 20 or less copies per cell, as opposed to high copy number plasmids that can have about 1000 copies per cell. High copy number plasmids and low copy number plasmids are well known in the art and it is understood that an exact plasmid copy per cell does not need to be known in order to characterize a plasmid as either high or low copy number.
In some cases, the decreasing expression level of a nucleic acid-guided nuclease, such as Cas9, Cpf1, MAD2, or MAD7, can increase transformation, editing, and/or recording efficiencies. In some cases, decreasing expression level of the nucleic acid-guided nuclease is done by expressing the nucleic acid-guided nuclease on a low copy number plasmid.
In some cases, the increasing expression level of a nucleic acid-guided nuclease, such as Cas9, Cpf1, MAD2, or MAD7, can increase transformation, editing, and/or recording efficiencies. In some cases, increasing expression level of the nucleic acid-guided nuclease is done by expressing the nucleic acid-guided nuclease on a high copy number plasmid.
Other methods of modulating the expression level of a protein are also envisioned and are known in the art. Such methods include using a inducible or constitutive promoter, incorporating enhancers or other expression regulatory elements onto an expression plasmid, using RNAi, amiRNAi, or other RNA silencing techniques to modulate transcript level, fusing the protein of interest to a degradation domain, or any other method known in the art.
Provided herein are methods for generating mutant libraries. In some examples, the mutant library can be effectively constructed and retrieved within 1-3 hours post recombineering. In some examples, the mutant library is constructed within 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, or 24 hours post recombineering. In some examples, the mutant library can be retrieved within 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 24, 36, or 48 hours post recombineering and/or post-constructing by recombineering.
Some methods disclosed herein can be used for trackable, precision genome editing. In some examples, methods disclosed herein can achieve high efficiency editing/mutating using a single cassette that encodes both an editing cassette and guide nucleic acid, and optionally a recorder cassette and second guide nucleic acid. Alternatively, a single vector can encode an editing cassette while a guide nucleic acid is provided sequentially or concomitantly. When used with parallel DNA synthesis, such as array-based DNA synthesis, methods disclosed herein can provide single step generation of hundreds or thousands of precision edits/mutations. Mutations can be mapped by sequencing the editing cassette on the vector, rather than by sequencing of the genome or a section of the genome of the cell or organism.
The methods disclosed herein can have broad utility in protein and genome engineering applications, as well as for reconstruction of mutations, such as mutations identified in laboratory evolution experiments. In some examples, the methods and compositions disclosed here can combine an editing cassette, which could include a desired mutation and a PAM mutation, with a gene encoding a guide nucleic acid on a single vector.
In some examples, a trackable mutant library can be generated in a single transformation or single reaction.
Methods disclosed herein can comprise introducing a cassette comprising an editing cassette that includes the desired mutation and the PAM mutation into a cell or population of cells. In some embodiments, the cell into which the cassette or vector is introduced also comprises a nucleic acid-guided nuclease, such as Cas9, Cpf1, MAD2, or MAD7. In some embodiments, a gene or mRNA encoding the nucleic acid-guided nuclease is concomitantly, sequentially, or subsequently introduced into the cell or population of cells. Expression of a targetable nuclease system, including nucleic acid-guided nuclease and a guide nucleic acid, in the cell or cell population can be activated such that the guide nucleic acid recruits the nucleic acid-guided nuclease to the target region, where dsDNA cleavage occurs.
In some examples, without wishing to be bound by any particular theory, the homologous region of an editing cassette complementary to the target sequence mutates the PAM and the one or more codon of the target sequence. Cells of the population of cells that did not integrate the PAM mutation can undergo unedited cell death due to nucleic acid-guided nuclease mediated dsDNA cleavage. In some examples, cells of the population of cells that integrate the PAM mutation do not undergo cell death; they remain viable and are selectively enriched to high abundance. Viable cells can be obtained and can provide a library of trackable or targeted mutations.
In some examples, without wishing to be bound by any particular theory, the homologous region of a recorder cassette complementary to the target sequence mutates the PAM and introduces a barcode into a target sequence. Cells of the population of cells that did not integrate the PAM mutation can undergo unedited cell death due to nucleic acid-guided nuclease mediated dsDNA cleavage. In some examples, cells of the population of cells that integrate the PAM mutation do not undergo cell death; they remain viable and are selectively enriched to high abundance. Viable cells can be obtained and can provide a library of trackable mutations.
A separate vector or mRNA encoding a nucleic acid-guided nuclease can be introduced into the cell or population of cells. Introducing a vector or mRNA into a cell or population of cells can be performed using any method or technique known in the art. For example, vectors can be introduced by standard protocols, such as transformation including chemical transformation and electroporation, transduction and particle bombardment. Additionally or alternatively, mRNA can be introduced by standard protocols, such as transformation as disclosed herein, and/or by techniques involving cell permeable peptides or nanoparticles.
An editing cassette can include (a) a region, which recognizes (hybridizes to) a target region of a nucleic acid in a cell or population of cells, is homologous to the target region of the nucleic acid of the cell and includes a mutation, referred to a desired mutation, of at least one nucleotide that can be in at least one codon relative to the target region, and (b) a protospacer adjacent motif (PAM) mutation. In some examples, the editing cassette also comprises a barcode. The barcode can be a unique barcode or relatively unique such that the corresponding mutation can be identified based on the barcode. The PAM mutation may be any insertion, deletion or substitution of one or more nucleotides that mutates the sequence of the PAM such that the mutated PAM (PAM mutation) is not recognized by a chosen nucleic acid-guided nuclease system. A cell that comprises such as a PAM mutation may be said to be “immune” to nucleic acid-guided nuclease-mediated killing. The desired mutation relative to the sequence of the target region may be an insertion, deletion, and/or substitution of one or more nucleotides and may be at least one codon of the target region. In some embodiments, the distance between the PAM mutation and the desired mutation is at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 40, 50, 60, 70, 80, 90, or 100 nucleotides on the editing cassette In some embodiments, the PAM mutation is located at least 9 nucleotides from the end of the editing cassette. In some embodiments, the desired mutation is located at least 9 nucleotides from the end of the editing cassette.
A desired mutation can be an insertion of a nucleic acid sequence relative to the sequence of the target sequence. The nucleic acid sequence inserted into the target sequence can be of any length. In some embodiments, the nucleic acid sequence inserted is at least 1, 2, 3, 4, 5, 10, 20, 30, 40, 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000, 1100, 1200, 1300, 1400, 1500, 1600, 1700, 1800, 1900, or at least 2000 nucleotides in length. In embodiments in which a nucleic acid sequence is inserted into the target sequence, the editing cassette comprises a region that is at least 10, 15, 20, 25, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50 51, 52, 53, 54, 55, 56, 57, 58, 59, or at least 60 nucleotides in length and homologous to the target sequence. The homology arms or homologous region can be about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, or more nucleotides in length, including any integer therein. The homology arms or homologous region can be over 200 nucleotides in length.
A barcode can be a unique barcode or relatively unique such that the corresponding mutation can be identified based on the barcode. In some examples, the barcode is a non-naturally occurring sequence that is not found in nature. In most examples, the combination of the desired mutation and the barcode within the editing cassette is non-naturally occurring and not found in nature. A barcode can be any number of nucleotides in length. A barcode can be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, or more than 30 nucleotides in length. In some cases, the barcode is more than 30 nucleotides in length.
An editing cassette or recorder cassette can comprise at least a portion of a gene encoding a guide nucleic acid, and optionally a promoter operable linked to the encoded guide nucleic acid. In some embodiments, the portion of the gene encoding the guide nucleic acid encodes the portion of the guide nucleic acid that is complementary to the target sequence. The portion of the guide nucleic acid that is complementary to the target sequence, or the guide sequence, can be at least 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or at least 30 nucleotides in length. In some embodiments, the guide sequence is 24 nucleotides in length. In some embodiments, the guide sequence is 18 nucleotides in length.
In some embodiments, the editing cassette or recorder cassette further comprises at least two priming sites. The priming sites may be used to amplify the cassette, for example by PCR. In some embodiments, the portion of the guide sequence is used as a priming site.
Editing cassettes or recorder cassettes for use in the described methods can be obtained or derived from many sources. For example, the cassettes can be synthesized, for example by array-based synthesis, multiplex synthesis, multi-parallel synthesis, PCR assembly, in vitro assembly, Gibson assembly, or any other synthesis method known in the art. In some embodiments, the editing cassette or recorder cassette is synthesized, for example by array-based synthesis, multiplex synthesis, multi-parallel synthesis, PCR assembly,—in vitro assembly, Gibson assembly, or any other synthesis method known in the art. The length of the editing cassette or recorder cassette may be dependent on the method used in obtaining said cassette.
An editing cassette can be approximately 50-300 nucleotides, 75-200 nucleotides, or between 80-120 nucleotides in length. In some embodiments, the editing cassette can be any discrete length between 50 nucleotide and 1 Mb.
A recorder cassette can be approximately 50-300 nucleotides, 75-200 nucleotides, or between 80-120 nucleotides in length. In some embodiments, the recorder cassette can be any discrete length between 50 nucleotide and 1 Mb.
Methods disclosed herein can also involve obtaining editing cassettes and recorder cassettes and constructing a trackable plasmid or vector. Methods of constructing a vector will be known to one ordinary skill in the art and may involve ligating the cassettes into a vector backbone. In some examples, plasmid construction occurs by in vitro DNA assembly methods, oligonucleotide assembly, PCR-based assembly, SLIC, CPEC, or other assembly methods well known in the art. In some embodiments, the cassettes or a subset (pool) of the cassettes can be amplified prior to construction of the vector, for example by PCR.
The cell or population of cells comprising a polynucleotide encoding a nucleic acid-guided nuclease can be maintained or cultured under conditions in which the nuclease is expressed. Nucleic acid-guided nuclease expression can be controlled or can be constitutively on. The methods described herein can involve maintaining cells under conditions in which nuclease expression is activated, resulting in production of the nuclease, for example, Cas9, Cpf1, MAD2, or MAD7. Specific conditions under which the nucleic acid-guided nuclease is expressed can depend on factors, such as the nature of the promoter used to regulate expression of the nuclease. Nucleic acid-guided nuclease expression can be induced in the presence of an inducer molecule, such as arabinose. When the cell or population of cells comprising nucleic acid-guided nuclease encoding DNA are in the presence of the inducer molecule, expression of the nuclease can occur. CRISPR-nuclease expression can be repressed in the presence of a repressor molecule. When the cell or population of cells comprising nucleic acid-guided nuclease encoding DNA are in the absence of a molecule that represses expression of the nuclease, expression of the nuclease can occur.
Cells or the population of cells that remain viable can be obtained or separated from the cells that undergo unedited cell death as a result of nucleic acid-guided nuclease-mediated killing; this can be done, for example, by spreading the population of cells on culture surface, allowing growth of the viable cells, which are then available for assessment.
Disclosed herein are methods for the identification of the mutation without the need to sequence the genome or large portions of the genome of the cell. The methods can involve sequencing of the editing cassette, recorder cassette, or barcode to identify the mutation of one of more codon. Sequencing of the editing cassette can be performed as a component of the vector or after its separation from the vector and, optionally, amplification. Sequencing can be performed using any sequencing method known in the art, such as by Sanger sequencing or next-generation sequencing methods.
Some methods described herein can be carried out in any type of cell in which a targetable nuclease system can function, or target and cleave DNA, including prokaryotic and eukaryotic cells. In some embodiments, the cell is a bacterial cell, such as Escherichia spp., e.g., E. coli. In other embodiments, the cell is a fungal cell, such as a yeast cell, e.g., Saccharomyces spp. In other embodiments, the cell is an algal cell, a plant cell, an insect cell, or a mammalian cell, including a human cell.
A “vector” is any of a variety of nucleic acids that comprise a desired sequence or sequences to be delivered to or expressed in a cell. A desired sequence can be included in a vector, such as by restriction and ligation or by recombination or assembly methods know in the art. Vectors are typically composed of DNA, although RNA vectors are also available. Vectors include, but are not limited to plasmids, fosmids, phagemids, virus genomes, artificial chromosomes, and synthetic nucleic acid molecules.
Vectors useful in the methods disclosed herein can comprise at least one editing cassette as described herein, at least one gene encoding a gRNA, and optionally a promoter and/or a barcode. More than one editing cassette can be included on the vector, for example 2, 3, 4, 5, 6, 7, 8, 9, 10 or more editing cassettes. The more than one editing cassettes can be designed to target different target regions, for example, there could be different editing cassettes, each of which contains at least one region homologous with a different target region. In other examples, each editing cassette target the same target region while each editing cassette comprises a different desired mutation relative to the target region. In other examples, the plurality of editing cassettes can comprise a combination of editing cassettes targeting the same target region and editing cassettes targeting different target regions. Each editing cassette can comprise an identifying barcode. Alternatively or additionally, the vector can include one or more genes encoding more than one gRNA, e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10 or more gRNAs. The more than one gRNAs can contain regions that are complementary to a portion of different target regions, for example, if there are different gRNAs, each of which can be complementary to a portion of a different target region. In other examples, the more than one gRNA can each target the same target region. In other examples, the more than one gRNA can be a combination of gRNAs targeting the same and different target regions.
A cassette comprising a gene encoding a portion of a guide nucleic acid, can be ligated or assembled into a vector that encodes another portion of a guide nucleic acid. Upon ligation or assembly, the portion of the guide nucleic acid from the cassette and the other portion of the guide nucleic acid can form a functional guide nucleic acid. A promoter and a gene encoding a guide nucleic acid can be operably linked.
In some embodiments, the methods involve introduction of a second vector encoding a nucleic acid-guided nuclease, such as Cas9, Cpf1, MAD2, or MAD7. The vector may further comprise one or more promoters operably linked to a gene encoding the nucleic acid-guided nuclease.
As used herein, “operably” linked can mean the promoter affects or regulates transcription of the DNA encoding a gene, such as the gene encoding the gRNA or the gene encoding a CRISPR nuclease.
A promoter can be a native promoter such as a promoter present in the cell into which the vector is introduced. A promoter can be an inducible or repressible promoter, for example, the promoter can be regulated allowing for inducible or repressible transcription of a gene, such as the gene encoding the guide nucleic acid or the gene encoding a nucleic acid-guided nuclease. Such promoters that are regulated by the presence or absence of a molecule can be referred to as an inducer or a repressor, respectively. The nature of the promoter needed for expression of the guide nucleic acid or nucleic acid-guided nuclease can vary based on the species or cell type and can be recognized by one of ordinary skill in the art.
A separate vector encoding a nucleic acid-guided nuclease can be introduced into a cell or population of cells before or at the same time as introduction of a trackable plasmid as disclosed herein. The gene encoding a nucleic acid-guided nuclease can be integrated into the genome of the cell or population of cells, or the gene can be maintained episomally. The nucleic acid-guided nuclease-encoding DNA can be integrated into the cellular genome before introduction of the trackable plasmid, or after introduction of the trackable plasmid. In some examples, a nucleic acid molecule, such as DNA-encoding a nucleic acid-guided nuclease, can be expressed from DNA integrated into the genome. In some embodiments, a gene encoding Cas9, Cpf1, MAD2, or MAD7 is integrated into the genome of the cell.
Vectors or cassettes useful in the methods described herein can further comprise two or more priming sites. In some embodiments, the presence of flanking priming sites allows amplification of the vector or cassette.
In some embodiments, a cassette or vector encodes a nucleic acid-guided nuclease comprising one or more nuclear localization sequences (NLSs), such as about or more than about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more NLSs. In some embodiments, the engineered nuclease comprises about or more than about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more NLSs at or near the amino-terminus, about or more than about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more NLSs at or near the carboxy-terminus, or a combination of these (e.g. one or more NLS at the amino-terminus and one or more NLS at the carboxy terminus). When more than one NLS is present, each may be selected independently of the others, such that a single NLS may be present in more than one copy and/or in combination with one or more other NLSs present in one or more copies. In a preferred embodiment of the invention, the engineered nuclease comprises at most 6 NLSs. In some embodiments, an NLS is considered near the N- or C-terminus when the nearest amino acid of the NLS is within about 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 40, 50, or more amino acids along the polypeptide chain from the N- or C-terminus. Non-limiting examples of NLSs include an NLS sequence derived from: the NLS of the SV40 virus large T-antigen, having the amino acid sequence PKKKRKV (SEQ ID NO: 111); the NLS from nucleoplasmin (e.g. the nucleoplasmin bipartite NLS with the sequence KRPAATKKAGQAKKKK (SEQ ID NO:112)); the c-myc NLS having the amino acid sequence PAAKRVKLD (SEQ ID NO:113) or RQRRNELKRSP (SEQ ID NO:114); the hRNPA1 M9 NLS having the sequence NQSSNFGPMKGGNFGGRSSGPYGGGGQYFAKPRNQGGY (SEQ ID NO: 115); the sequence RMRIZFKNKGKDTAELRRRRVEVSVELRKAKKDEQILKRRNV (SEQ ID NO: 116) of the IBB domain from importin-alpha; the sequences VSRKRPRP (SEQ ID NO:117) and PPKKARED (SEQ ID NO:11 of the myoma T protein; the sequence PQPKKKPL (SEQ ID NO:119) of human p53; the sequence SALIKKKKKMAP (SEQ ID NO:120) of mouse c-abl IV; the sequences DRLRR (SEQ ID NO:121) and PKQKKRK (SEQ ID NO:122) of the influenza virus NS1; the sequence RKLKKKIKKL (SEQ ID NO:123) of the Hepatitis virus delta antigen; the sequence REKKKFLKRR (SEQ ID NO: 124) of the mouse M×1 protein; the sequence KRKGDEVDGVDEVAKKKSKK (SEQ ID NO: 125) of the human poly(ADP-ribose) polymerase; and the sequence RKCLQAGMNLEARKTKK (SEQ ID NO: 126) of the steroid hormone receptors (human) glucocorticoid.
In general, the one or more NLSs are of sufficient strength to drive accumulation of the nucleic acid-guided nuclease in a detectable amount in the nucleus of a eukaryotic cell. In general, strength of nuclear localization activity may derive from the number of NLSs, the particular NLS(s) used, or a combination of these factors. Detection of accumulation in the nucleus may be performed by any suitable technique. For example, a detectable marker may be fused to the nucleic acid-guided nuclease, such that location within a cell may be visualized, such as in combination with a means for detecting the location of the nucleus (e.g. a stain specific for the nucleus such as DAPI). Cell nuclei may also be isolated from cells, the contents of which may then be analyzed by any suitable process for detecting protein, such as immunohistochemistry, Western blot, or enzyme activity assay. Accumulation in the nucleus may also be determined indirectly, such as by an assay for the effect of the nucleic acid-guided nuclease complex formation (e.g. assay for DNA cleavage or mutation at the target sequence, or assay for altered gene expression activity affected by targetable nuclease complex formation and/or nucleic acid-guided nuclease activity), as compared to a control not exposed to the nucleic acid-guided nuclease or targetable nuclease complex, or exposed to a nucleic acid-guided nuclease lacking the one or more NLSs.

ProSAR

Methods disclosed herein are capable of engineering a few to hundreds of genetic sequence or proteins simultaneously. These methods can permit one to map in a single experiment many or all possible residue changes over a collection of desired proteins onto a trait of interest, as part of individual proteins of interest or as part of a pathway. This approach can be used at least for the following by mapping i) any number of residue changes for any number of proteins of interest in a specific biochemical pathway or that catalyze similar reactions or ii) any number of residues in the regulatory sites of any number of proteins or interest with a specific regulon or iii) any number of residues of a biological agent used to treat a health condition.
In some embodiments, methods described herein include identifying genetic variations of one or more target genes that affect any number or residues, such as one or more, or all residues of one or more target proteins. In accordance with these embodiments, compositions and methods disclosed herein permit parallel analysis of two or more target proteins or proteins that contribute to a trait. Parallel analysis of multiple proteins by a single experiment described can facilitate identification, modification and design of superior systems for example for producing a eukaryotic or prokaryotic byproduct, producing a eukaryotic byproduct, for example, a biological agent such as a growth factor or antibody, in a prokaryotic organism and the like. Relevant biologics used in analysis and treatment of disease can be produced in these genetically engineered environments that could reduce production time, increase quality all while reducing costs to the manufacturers and the consumers.
Some embodiments disclosed herein comprise constructs of use for studying genetic variations of a gene or gene segment wherein the gene or gene segment is capable of generating a protein. A construct can be generated for any number of residues, such as one, two, more than two, or all residue modifications of a target protein that is linked to a trackable agent such as a barcode. A barcode indicative of a genetic variation of a gene of a target protein can be located outside of the open reading frame of the gene. In some embodiments such a barcode can be located many hundreds or thousands of bases away from the gene. It is contemplated herein that these methods can be performed in vivo. In some examples, such a construct comprises a trackable polynucleic acid or plasmid as disclosed herein.
Constructs described herein can be used to compile a comprehensive library of genetic variations encompassing all residue changes of one target protein, more than one target protein or target proteins that contribute to a trait. In certain embodiments, libraries disclosed herein can be used to select proteins with improved qualities to create an improved single or multiple protein system for example for producing a byproduct, such as a chemical, biofuels, biological agent, pharmaceutical agent, or for biomass, or biologic compared to a non-selective system.

Protein Sequence Activity Relationship (ProSAR) Mapping

Understanding the relationship between a protein's amino acid structure and its overall function continues to be of great practical, clinical, and scientific significance for biologists and engineers. Directed evolution can be a powerful engineering and discovery tool, but the random and often combinatorial nature of mutations makes their individual impacts difficult to quantify and thus challenges further engineering. More systematic analysis of contributions of individual residues or saturation mutagenesis remains labor- and time-intensive for entire proteins and simply is not possible on reasonable timescales for multiple proteins in parallel, such as metabolic pathways or multi-protein complexes, using standard methods.
Provided herein are methods which can be used to rapidly and efficiently examine the roles of some or all genes in a viral, microbial, or eukaryotic genome using mixtures of barcoded oligonucleotides. In some embodiments, these compositions and methods can be used to develop a powerful new technology for comprehensively mapping protein structure-activity relationships (ProSAR).
Using methods and compositions disclosed herein, multiplex cassette synthesis can be combined with recombineering, to create mutant libraries of specifically designed and barcoded mutations along one or more genes of interest in parallel. Screens and/or selections followed by high-throughput sequencing and/or barcode microarray methods can allow for rapid mapping of protein sequence-activity relationships (ProSAR). In some embodiments, systematic ProSAR mapping can elucidate individual amino acid mutations for improved function and/or activity and/or stability etc.
Methods can be iterated to combinatorially improve the function, activity, or stability. Cassettes can be generated by oligonucleotide synthesis. Given that existing capabilities of multiplex oligonucleotide synthesis can reach over 120,000 oligonucleotides per array, combined with recombineering, the methods disclosed herein can be scaled to construct mutant libraries for dozens to hundreds of proteins in a single experiment. In some examples, at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 25, 50, 75, 100, 150, 200, 250, 300, 400, 500, 600, 700, 800, 900, 1000, or more proteins can be partially or completely covered by mutant libraries generated by the methods disclosed herein.
Disclosed herein are strategies to construct barcoded substitution libraries for several different proteins at the same time. Using existing multiplex DNA synthesis technology, as disclosed, a partial or complete substitution library for one or more protein constructs can be barcoded, or non-barcoded if desired, for one or for several hundred proteins at the same time. In some examples, such libraries comprise trackable plasmids as disclosed herein.
Some embodiments herein apply to analysis and structure/function/stability library construction of any protein with a corresponding screen or selection for activity. Cassette library size can depend on the number (N) of amino acids in a protein of interest, with a full saturation library, including all 20 amino acids at each position and optionally non-naturally occurring amino acids, scaling as 19 (or more)×N and an alanine-mapping library scaling as 1×N. Thus, in some examples, screening of even very large proteins of more than 1,000 amino acids can be tractable given current multiplex oligo synthesis capabilities of at least 120,000 oligos per array.
In addition or as an alternative to activity screens, more general properties with developed high-throughput screens and selections can be efficiently tested using methods and cassettes disclosed herein. For example, universal protein folding and solubility reporters can be engineered for expression in the cytoplasm, periplasm, and the inner membrane. In some examples, a protein library can be screened under different conditions such as different temperatures, different substrates or co-factors, in order to identify residue changes required for expression of various traits. In other embodiments, because residues can be analyzed one at a time, mutations at residues important for a particular trait, such as thermostability, resistance to environmental pressures, or increases or decreases in functionality or production, can be combined via multiplex recombineering with mutations important for various other traits, such as catalytic activity, to create combinatorial libraries for multi-trait optimization.
Methods disclosed herein can provide for creating and/or evaluating comprehensive, in vivo, mutational libraries of one or more target protein(s). These approaches can be extended via a recorder cassettes or barcoding technology to generate trackable mutational libraries for any number of residues or every residue in a protein. This approach can be based on protein sequence-activity relationship mapping method extended to work in vivo, capable of working on one or a few to hundreds of proteins simultaneously depending on the technology selected. For example, these methods permit one to map in a single experiment any number of, the majority of, or all possible residue changes over a collection of desired proteins onto a trait of interest, as part of individual proteins of interest or as part of a pathway.
In some examples, these approaches can be used at least for the following by mapping i) any number of or all residue changes for any number of or all proteins in a specific biochemical pathway, such as lycopene production, or that catalyze similar reactions, such as dehydrogenases or other enzymes of a pathway of use to produce a desired effect or produce a product, or ii) any number of or all residues in the regulatory sites of any number of or all proteins with a specific regulatory mechanism, such as heat shock response, or iii) any number of or all residues of a biological agent used to treat a health condition, such as insulin, a growth factor (HCG), an anti-cancer biologic, or a replacement protein for a deficient population.
Scores related to various input parameters can be assigned in order to generate one or more composite score(s) for designing genomically-engineered organisms or systems. These scores can reflect quality of genetic variations in genes or genetic loci as they relate to selection of an organism or design of an organism for a predetermined production, trait or traits. Certain organisms or systems can be designed based on a need for improved organisms for biorefining, biomass, such as crops, trees, grasses, crop residues, or forest residues, biofuel production, and using biological conversion, fermentation, chemical conversion and catalysis to generate and use compounds, biopharmaceutical production and biologic production. In certain embodiments, this can be accomplished by modulating growth or production of microorganism through genetic manipulation methods disclosed herein.
Genetic manipulation by methods disclosed herein of genes encoding a protein can be used to make desired genetic changes that can result in desired phenotypes and can be accomplished through numerous techniques including but not limited to, i) introduction of new genetic material, ii) genetic insertion, disruption or removal of existing genetic material, as well as, iii) mutation of genetic material, such as a point mutation, or any combinations of i, ii, and iii, that results in desired genetic changes with desired phenotypic changes. Mutations can be directed or random, in addition to those including, but not limited to, error prone or directed mutagenesis through PCR, mutator strains, and random mutagenesis. Mutations can be incorporated using trackable plasmids and methods as disclosed herein.
Disclosed methods can be used for inserting and accumulating higher order modifications into a microorganism's genome or a target protein; for example, multiple different site-specified mutations in the same genome, at high efficiency to generate libraries of genomes with over 1, 5, 10, 15, 20, 25, 30, 40, 50, 60, 70, 80, 90, 100, 150, 200, 250, 300, or more targeted modifications are described. In some examples, these mutations are within regulatory modules, regulatory elements, protein-coding regions, or non-coding regions. Protein coding modifications can include, but are not limited to, amino acid changes, codon optimization, and translation tuning.
In some instances, methods are provided for the co-delivery of reagents to a single biological cell. The methods generally involve the attachment or linkage of two or more cassettes, followed by delivery of the linked cassettes to a single cell. Generally, the methods provided herein involve the delivery of two or more cassettes to a single cell. In many cases, it is desirable that each individual cell receives the two or more cassettes. Traditional methods of reagent delivery may often be inefficient and/or inconsistent, leading to situations in which some cells receive only one of the cassettes. The methods provided herein may improve the efficiency and/or consistency of reagent delivery, such that a majority of cells in a cell population each receive the two or more cassettes. For example, more than 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% of the cells in a cell population may receive the two or more cassettes.
The two or more cassettes may be linked by any known method in the art and generally the method chosen will be commensurate with the chemistry of the cassettes. Generally, the two or more cassettes are linked by a covalent bond (i.e., covalently-linked), however, other types of non-covalent chemical bonds are envisioned, such as hydrogen bonds, ionic bonds, and metallic bonds. In this way, the editing cassette and the recorder cassette may be linked and delivered into a single cell. A known edit is then associated with a known recorder or barcode sequence for that cell.
In one example, the two or more cassettes are nucleic acids, such as two or more nucleic acids. The nucleic acids may be RNA, DNA, or a combination of both, and may contain any number of chemically-modified nucleotides or nucleotide analogues. In some cases, two or more RNA cassettes are linked for delivery to a single cell. In other cases, two or more DNA cassettes are linked for delivery to a single cell. In yet other cases, a DNA cassettes and an RNA cassettes are linked for delivery to a single cell. The nucleic acids may be derived from genomic RNA, complementary DNA (cDNA), or chemically or enzymatically synthesized DNA.
A cassettes may be of 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, about 110, about 120, about 130, about 140, about 150, about 160, about 170, about 180, about 190, about 200, about 210, about 220, about 230, about 240, about 250, about 275, about 300, about 325, about 350, about 375, about 400, about 425, about 450, about 475, about 500, about 525, about 550, about 575, about 600, about 625, about 650, about 675, about 700, about 725, about 750, about 775, about 800, about 825, about 850, about 875, about 900, about 925, about 950, about 975, about 1000, about 1100, about 1200, about 1300, about 1400, about 1500, about 1750, about 2000, about 2500, about 3000, about 4000, about 5000, about 6000, about 7000, about 8000, about 9000, about 10,000 or greater nucleotide residues in length, up to a full length protein encoding or regulatory genetic element.
Two or more cassettes may be linked on a linear nucleic acid molecule or may be linked on a plasmid or circular nucleic acid molecule. The two or more cassettes may be linked directly to one another or may be separated by one or more nucleotide spacers or linkers.
Two or more cassettes may be covalently linked on a linear cassettes or may be covalently linked on a plasmid or circular nucleic acid molecule. The two or more cassettes may be covalently linked directly to one another or may be separated by one or more nucleotide spacers or linkers.
Any number and variety of cassettes may be linked for co-delivery. For example, the two or more cassettes may include nucleic acids, lipids, proteins, peptides, small molecules, or any combination thereof. The two or more cassettes may be essentially any cassettes that are amenable to linkage.
In preferred examples, the two or more cassettes are covalently linked (e.g., by a chemical bond). Covalent linkage may help to ensure that the two or more cassettes are co-delivered to a single cell. Generally, the two or more cassettes are covalently linked prior to delivery to a cell. Any method of covalently linking two or more molecules may be utilized, and it should be understood that the methods used will be at least partly determined by the types of cassettes to be linked.
In some instances, methods are provided for the co-delivery of reagents to a single biological cell. The methods generally involve the covalent attachment or linkage of two or more cassettes, followed by delivery of the covalently-linked cassettes into a single cell. The methods provided may help to ensure that an individual cell receives the two or more cassettes. Any known method of reagent delivery may be utilized to deliver the linked cassettes to a cell and will at least partly depend on the chemistry of the cassettes to be delivered. Non-limiting examples of reagent delivery methods may include: transformation, lipofection, electroporation, transfection, nanoparticles, and the like.
In various embodiments, cassettes, or isolated, donor, or editing nucleic acids may be introduced to a cell or microorganism to alter or modulate an aspect of the cell or microorganism, for example survival or growth of the microorganism as disclosed herein. The isolated nucleic acid may be derived from genomic RNA, complementary DNA (cDNA), chemically or enzymatically synthesized DNA. Additionally or alternatively, isolated nucleic acids may be of use for capture probes, primers, labeled detection oligonucleotides, or fragments for DNA assembly.
A “nucleic acid” can include single-stranded and/or double-stranded molecules, as well as DNA, RNA, chemically modified nucleic acids and nucleic acid analogs. It is contemplated that a nucleic acid may be of 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, about 110, about 120, about 130, about 140, about 150, about 160, about 170, about 180, about 190, about 200, about 210, about 220, about 230, about 240, about 250, about 275, about 300, about 325, about 350, about 375, about 400, about 425, about 450, about 475, about 500, about 525, about 550, about 575, about 600, about 625, about 650, about 675, about 700, about 725, about 750, about 775, about 800, about 825, about 850, about 875, about 900, about 925, about 950, about 975, about 1000, about 1100, about 1200, about 1300, about 1400, about 1500, about 1750, about 2000, about 2500, about 3000, about 4000, about 5000, about 6000, about 7000, about 8000, about 9000, about 10,000 or greater nucleotide residues in length, up to a full length protein encoding or regulatory genetic element.
Isolated nucleic acids may be made by any method known in the art, for example using standard recombinant methods, assembly methods, synthetic techniques, or combinations thereof. In some embodiments, the nucleic acids may be cloned, amplified, assembled, or otherwise constructed.
The nucleic acids may conveniently comprise sequences in addition to a portion of a lysine riboswitch. For example, a multi-cloning site comprising one or more endonuclease restriction sites may be added. A nucleic acid may be attached to a vector, adapter, or linker for cloning of a nucleic acid. Additional sequences may be added to such cloning and sequences to optimize their function, to aid in isolation of the nucleic acid, or to improve the introduction of the nucleic acid into a cell. Use of cloning vectors, expression vectors, adapters, and linkers is well known in the art.
Isolated nucleic acids may be obtained from cellular, bacterial, or other sources using any number of cloning methodologies known in the art. In some embodiments, oligonucleotide probes which selectively hybridize, under stringent conditions, to other oligonucleotides or to the nucleic acids of an organism or cell. Methods for construction of nucleic acid libraries are known and any such known methods may be used.
Cellular genomic DNA, RNA, or cDNA may be screened for the presence of an identified genetic element of interest using a probe based upon one or more sequences. Various degrees of stringency of hybridization may be employed in the assay.
High stringency conditions for nucleic acid hybridization are well known in the art. For example, conditions may comprise low salt and/or high temperature conditions, such as provided by about 0.02 M to about 0.15 M NaCl at temperatures of about 50° C. to about 70° C. It is understood that the temperature and ionic strength of a desired stringency are determined in part by the length of the particular nucleic acid(s), the length and nucleotide content of the target sequence(s), the charge composition of the nucleic acid(s), and by the presence or concentration of formamide, tetramethylammonium chloride or other solvent(s) in a hybridization mixture. Nucleic acids may be completely complementary to a target sequence or may exhibit one or more mismatches.
Nucleic acids of interest may also be amplified using a variety of known amplification techniques. For instance, polymerase chain reaction (PCR) technology may be used to amplify target sequences directly from DNA, RNA, or cDNA. PCR and other in vitro amplification methods may also be useful, for example, to clone nucleic acid sequences, to make nucleic acids to use as probes for detecting the presence of a target nucleic acid in samples, for nucleic acid sequencing, or for other purposes.
Isolated nucleic acids may be prepared by direct chemical synthesis by methods such as the phosphotriester method, or using an automated synthesizer. Chemical synthesis generally produces a single stranded oligonucleotide. This may be converted into double stranded DNA by hybridization with a complementary sequence or by polymerization with a DNA polymerase using the single strand as a template.
Any method known in the art for identifying, isolating, purifying, using and assaying activities of target proteins contemplated herein are contemplated. Target proteins contemplated herein include protein agents used to treat a human condition or to regulate processes (e.g. part of a pathway such as an enzyme) involved in disease of a human or non-human mammal. Any method known for selection and production of antibodies or antibody fragments is also contemplated. Additionally or alternatively, target proteins can be proteins or enzymes involved in a pathway or process in a virus, cell, or organism.

Targetable Nucleic Acid Cleavage Systems

Some methods disclosed herein comprise targeting cleavage of specific nucleic acid sequences using a site-specific, targetable, and/or engineered nuclease or nuclease system. Such nucleases can create double-stranded break (DSBs) at desired locations in a genome or nucleic acid molecule. In other examples, a nuclease can create a single strand break. In some cases, two nucleases are used, each of which generates a single strand break.
The one or more double or single strand break can be repaired by natural processes of homologous recombination (HR) and non-homologous end-joining (NHEJ) using the cell's endogenous machinery. Additionally or alternatively, endogenous or heterologous recombination machinery can be used to repair the induced break or breaks.
Engineered nucleases such as zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), engineered homing endonucleases, and RNA or DNA guided endonucleases, such as CRISPR/Cas such as Cas9 or CPF1, and/or Argonaute systems, are particularly appropriate to carry out some of the methods of the present invention. Additionally or alternatively, RNA targeting systems can use used, such as CRISPR/Cas systems including c2c2 nucleases.
Methods disclosed herein can comprise cleaving a target nucleic acid using a CRISPR systems, such as a Type I, Type II, Type III, Type IV, Type V, or Type VI CRISPR system. CRISPR/Cas systems can be multi-protein systems or single effector protein systems. Multi-protein, or Class 1, CRISPR systems include Type I, Type III, and Type IV systems. Alternatively, Class 2 systems include a single effector molecule and include Type II, Type VI, and Type VI.
CRISPR systems used in methods disclosed herein can comprise a single or multiple effector proteins. An effector protein can comprise one or multiple nuclease domains. An effector protein can target DNA or RNA, and the DNA or RNA may be single stranded or double stranded. Effector proteins can generate double strand or single strand breaks. Effector proteins can comprise mutations in a nuclease domain thereby generating a nickase protein. Effector proteins can comprise mutations in one or more nuclease domains, thereby generating a catalytically dead nuclease that is able to bind but not cleave a target sequence. CRISPR systems can comprise a single or multiple guiding RNAs. The gRNA can comprise a crRNA. The gRNA can comprise a chimeric RNA with crRNA and tracrRNA sequences. The gRNA can comprise a separate crRNA and tracrRNA. Target nucleic acid sequences can comprise a protospacer adjacent motif (PAM) or a protospacer flanking site (PFS). The PAM or PFS may be 3′ or 5′ of the target or protospacer site. Cleavage of a target sequence may generate blunt ends, 3′ overhangs, or 5′ overhangs.
A gRNA can comprise a spacer sequence. Spacer sequences can be complementary to target sequences or protospacer sequences. Spacer sequences can be 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, or 36 nucleotides in length. In some examples, the spacer sequence can be less than 10 or more than 36 nucleotides in length.
A gRNA can comprise a repeat sequence. In some cases, the repeat sequence is part of a double stranded portion of the gRNA. A repeat sequence can be 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 nucleotides in length. In some examples, the spacer sequence can be less than 10 or more than 50 nucleotides in length.
A gRNA can comprise one or more synthetic nucleotides, non-naturally occurring nucleotides, nucleotides with a modification, deoxyribonucleotide, or any combination thereof. Additionally or alternatively, a gRNA may comprise a hairpin, linker region, single stranded region, double stranded region, or any combination thereof. Additionally or alternatively, a gRNA may comprise a signaling or reporter molecule.
A CRISPR nuclease can be endogenously or recombinantly expressed within a cell. A CRISPR nuclease can be encoded on a chromosome, extrachromosomally, or on a plasmid, synthetic chromosome, or artificial chromosome. A CRISPR nuclease can be provided or delivered to the cell as a polypeptide or mRNA encoding the polypeptide. In such examples, polypeptide or mRNA can be delivered through standard mechanisms known in the art, such as through the use of cell permeable peptides, nanoparticles, or viral particles.
gRNAs can be encoded by genetic or episomal DNA within a cell. In some examples, gRNAs can be provided or delivered to a cell expressing a CRISPR nuclease. gRNAs can be provided or delivered concomitantly with a CRISPR nuclease or sequentially. Guide RNAs can be chemically synthesized, in vitro transcribed, or otherwise generated using standard RNA generation techniques known in the art.
A CRISPR system can be a Type II CRISPR system, for example a Cas9 system. The Type II nuclease can comprise a single effector protein, which, in some cases, comprises a RuvC and HNH nuclease domains. In some cases a functional Type II nuclease can comprise two or more polypeptides, each of which comprises a nuclease domain or fragment thereof. The target nucleic acid sequences can comprise a 3′ protospacer adjacent motif (PAM). In some examples, the PAM may be 5′ of the target nucleic acid. Guide RNAs (gRNA) can comprise a single chimeric gRNA, which contains both crRNA and tracrRNA sequences. Alternatively, the gRNA can comprise a set of two RNAs, for example a crRNA and a tracrRNA. The Type II nuclease can generate a double strand break, which is some cases creates two blunt ends. In some cases, the Type II CRISPR nuclease is engineered to be a nickase such that the nuclease only generates a single strand break. In such cases, two distinct nucleic acid sequences can be targeted by gRNAs such that two single strand breaks are generated by the nickase. In some examples, the two single strand breaks effectively create a double strand break. In some cases where a Type II nickase is used to generate two single strand breaks, the resulting nucleic acid free ends can either be blunt, have a 3′ overhang, or a 5′ overhang. In some examples, a Type II nuclease may be catalytically dead such that it binds to a target sequence, but does not cleave. For example, a Type II nuclease could have mutations in both the RuvC and HNH domains, thereby rendering the both nuclease domains non-functional. A Type II CRISPR system can be one of three sub-types, namely Type II-A, Type II-B, or Type II-C.
A CRISPR system can be a Type V CRISPR system, for example a Cpf1, C2c1, or C2c3 system. The Type V nuclease can comprise a single effector protein, which in some cases comprises a single RuvC nuclease domain. In other cases, a function Type V nuclease comprises a RuvC domain split between two or more polypeptides. In such cases, the target nucleic acid sequences can comprise a 5′ PAM or 3′ PAM. Guide RNAs (gRNA) can comprise a single gRNA or single crRNA, such as can be the case with Cpf1. In some cases, a tracrRNA is not needed. In other examples, such as when C2c1 is used, a gRNA can comprise a single chimeric gRNA, which contains both crRNA and tracrRNA sequences or the gRNA can comprise a set of two RNAs, for example a crRNA and a tracrRNA. The Type V CRISPR nuclease can generate a double strand break, which in some cases generates a 5′ overhang. In some cases, the Type V CRISPR nuclease is engineered to be a nickase such that the nuclease only generates a single strand break. In such cases, two distinct nucleic acid sequences can be targeted by gRNAs such that two single strand breaks are generated by the nickase. In some examples, the two single strand breaks effectively create a double strand break. In some cases where a Type V nickase is used to generate two single strand breaks, the resulting nucleic acid free ends can either be blunt, have a 3′ overhang, or a 5′ overhang. In some examples, a Type V nuclease may be catalytically dead such that it binds to a target sequence, but does not cleave. For example, a Type V nuclease could have mutations a RuvC domain, thereby rendering the nuclease domain non-functional.
A CRISPR system can be a Type VI CRISPR system, for example a C2c2 system. A Type VI nuclease can comprise a HEPN domain. In some examples, the Type VI nuclease comprises two or more polypeptides, each of which comprises a HEPN nuclease domain or fragment thereof. In such cases, the target nucleic acid sequences can by RNA, such as single stranded RNA. When using Type VI CRISPR system, a target nucleic acid can comprise a protospacer flanking site (PFS). The PFS may be 3′ or 5′ or the target or protospacer sequence. Guide RNAs (gRNA) can comprise a single gRNA or single crRNA. In some cases, a tracrRNA is not needed. In other examples, a gRNA can comprise a single chimeric gRNA, which contains both crRNA and tracrRNA sequences or the gRNA can comprise a set of two RNAs, for example a crRNA and a tracrRNA. In some examples, a Type VI nuclease may be catalytically dead such that it binds to a target sequence, but does not cleave. For example, a Type VI nuclease could have mutations in a HEPN domain, thereby rendering the nuclease domains non-functional.
Non-limiting examples of suitable nucleases, including nucleic acid-guided nucleases, for use in the present disclosure include C2c1, C2c2, C2c3, Cas1, Cas1B, Cas2, Cas3, Cas4, Cas5, Cas6, Cas7, Cas8, Cas9 (also known as Csn1 and Csx12), Cas10, Cpf1, Csy1, Csy2, Csy3, Cse1, Cse2, Csc1, Csc2, Csa5, Csn2, Csm2, Csm3, Csm4, Csm5, Csm6, Cmr1, Cmr3, Cmr4, Cmr5, Cmr6, Csb1, Csb2, Csb3, Csx17, Csx14, Csx100, Csx16, CsaX, Csx3, Csx1, Csx15, Csf1, Csf2, Csf3, Csf4, homologues thereof, orthologues thereof, or modified versions thereof. Suitable nucleic acid-guided nucleases can be from an organism from a genus which includes but is not limited to Thiomicrospira, Succinivibrio, Candidatus, Porphyromonas, Acidomonococcus, Prevotella, Smithella, Moraxella, Synergistes, Francisella, Leptospira, Catenibacterium, Kandleria, Clostridium, Dorea, Coprococcus, Enterococcus, Fructobacillus, Weissella, Pediococcus, Corynebacter, Sutterella, Legionella, Treponema, Roseburia, Filifactor, Eubacterium, Streptococcus, Lactobacillus, Mycoplasma, Bacteroides, Flaviivola, Flavobacterium, Sphaerochaeta, Azospirillum, Gluconacetobacter, Neisseria, Roseburia, Parvibaculum, Staphylococcus, Nitratifractor, Mycoplasma, Alicyclobacillus, Brevibacilus, Bacillus, Bacteroidetes, Brevibacilus, Carnobacterium, Clostridiaridium, Clostridium, Desulfonatronum, Desulfovibrio, Helcococcus, Leptotrichia, Listeria, Methanomethyophilus, Methylobacterium, Opitutaceae, Paludibacter, Rhodobacter, Sphaerochaeta, Tuberibacillus, and Campylobacter. Species of organism of such a genus can be as otherwise herein discussed. Suitable nucleic acid-guided nucleases can be from an organism from a genus or unclassified genus within a kingdom, which includes but is not limited to Firmicute, Actinobacteria, Bacteroidetes, Proteobacteria, Spirochates, and Tenericutes. Suitable nucleic acid-guided nucleases can be from an organism from a genus or unclassified genus within a phylum which includes but is not limited to Erysipelotrichia, Clostridia, Bacilli, Actinobacteria, Bacteroidetes, Flavobacteria, Alphaproteobacteria, Betaproteobacteria, Gammaproteob acteria, Deltaproteob acteria, Epsilonproteobacteria, Spirochaetes, and Mollicutes. Suitable nucleic acid-guided nucleases can be from an organism from a genus or unclassified genus within an order which includes but is not limited to Clostridiales, Lactobacillales, Actinomycetales, Bacteroidales, Flavobacteriales, Rhizobiales, Rhodospirillales, Burkholderiales, Neisseriales, Legionellales, Nautiliales, Campylobacterales, Spirochaetales, Mycoplasmatales, and Thiotrichales. Suitable nucleic acid-guided nucleases can be from an organism from a genus or unclassified genus within a family which includes but is not limited to Lachnospiraceae, Enterococcaceae, Leuconostocaceae, Lactobacillaceae, Streptococcaceae, Peptostreptococcaceae, Staphylococcaceae, Eubacteriaceae, Corynebacterineae, Bacteroidaceae, Flavobacterium, Cryomoorphaceae, Rhodobiaceae, Rhodospirillaceae, Acetobacteraceae, Sutterellaceae, Neisseriaceae, Legionellaceae, Nautiliaceae, Campylobacteraceae, Spirochaetaceae, Mycoplasmataceae, Pisciririckettsiaceae, and Francisellaceae.
Other nucleic acid-guided nucleases suitable for use in the methods, systems, and compositions of the present disclosure include those derived from an organism such as, but not limited to, Thiomicrospira sp. XS5, Eubacterium rectale, Succinivibrio dextrinosolvens, Candidatus Methanoplasma termitum, Candidatus Methanomethylophilus alvus, Porphyromonas crevioricanis, Flavobacterium branchiophilum, Acidomonococcus sp., Lachnospiraceae bacterium COE1, Prevotella brevis ATCC 19188, Smithella sp. SCADC, Moraxella bovoculi, Synergistes jonesii, Bacteroidetes oral taxon 274, Francisella tularensis, Leptospira inadai serovar Lyme str. 10, Acidomonococcus sp. crystal structure (5B43) S. mutans, S. agalactiae, S. equisimilis, S. sanguinis, S. pneumonia; C. jejuni, C. coli; N. salsuginis, N. tergarcus; S. auricularis, S. carnosus; N. meningitides, N. gonorrhoeae; L. monocytogenes, L. ivanovii; C. botulinum, C. difficile, C. tetani, C. sordellii; Francisella tularensis 1, Prevotella albensis, Lachnospiraceae bacterium MC 2017 1, Butyrivibrio proteoclasticus, Peregrinibacteria bacterium GW2011_GWA2_33_10, Parcubacteria bacterium GW2011_GWC2_44_17, Smithella sp. SCADC, Acidaminococcus sp. BV3L6, Lachnospiraceae bacterium MA2020, Candidatus Methanoplasma termitum, Eubacterium eligens, Moraxella bovoculi 237, Leptospira inadai, Lachnospiraceae bacterium ND2006, Porphyromonas crevioricanis 3, Prevotella disiens, Porphyromonas macacae, Catenibacterium sp. CAG:290, Kandleria vitulina, Clostridiales bacterium KA00274, Lachnospiraceae bacterium 3-2, Dorea longicatena, Coprococcus catus GD/7, Enterococcus columbae DSM 7374, Fructobacillus sp. EFB-N1, Weissella halotolerans, Pediococcus acidilactici, Lactobacillus curvatus, Streptococcus pyogenes, Lactobacillus versmoldensis, and Filifactor alocis ATCC 35896.
Suitable nucleases for use in any of the methods disclosed herein include, but are not limited to, nucleases having the sequences listed in Table 1, or homologues having at least 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 99% sequence identity to any of the nucleases listed in Table 1.

	TABLE 1

	MAD	Amino acid
	nuclease	sequence

	MAD1	SEQ ID NO: 1
	MAD2	SEQ ID NO: 2
	MAD3	SEQ ID NO: 3
	MAD4	SEQ ID NO: 4
	MAD5	SEQ ID NO: 5
	MAD6	SEQ ID NO: 6
	MAD7	SEQ ID NO: 7
	MAD8	SEQ ID NO: 8
	MAD9	SEQ ID NO: 9
	MAD10	SEQ ID NO: 10
	MAD11	SEQ ID NO: 11
	MAD12	SEQ ID NO: 12
	MAD13	SEQ ID NO: 13
	MAD14	SEQ ID NO: 14
	MAD15	SEQ ID NO: 15
	MAD16	SEQ ID NO: 16
	MAD17	SEQ ID NO: 17
	MAD18	SEQ ID NO: 18
	MAD19	SEQ ID NO: 19
	MAD20	SEQ ID NO: 20

In some methods disclosed herein, Argonaute (Ago) systems can be used to cleave target nucleic acid sequences. Ago protein can be derived from a prokaryote, eukaryote, or archaea. The target nucleic acid may be RNA or DNA. A DNA target may be single stranded or double stranded. In some examples, the target nucleic acid does not require a specific target flanking sequence, such as a sequence equivalent to a protospacer adjacent motif or protospacer flanking sequence. The Ago protein may create a double strand break or single strand break. In some examples, when a Ago protein forms a single strand break, two Ago proteins may be used in combination to generate a double strand break. In some examples, an Ago protein comprises one, two, or more nuclease domains. In some examples, an Ago protein comprises one, two, or more catalytic domains. One or more nuclease or catalytic domains may be mutated in the Ago protein, thereby generating a nickase protein capable of generating single strand breaks. In other examples, mutations in one or more nuclease or catalytic domains of an Ago protein generates a catalytically dead Ago protein that can bind but not cleave a target nucleic acid.
Ago proteins can be targeted to target nucleic acid sequences by a guiding nucleic acid. In many examples, the guiding nucleic acid is a guide DNA (gDNA). The gDNA can have a 5′ phosphorylated end. The gDNA can be single stranded or double stranded. Single stranded gDNA can be 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 nucleotides in length. In some examples, the gDNA can be less than 10 nucleotides in length. In some examples, the gDNA can be more than 50 nucleotides in length.
Argonaute-mediated cleavage can generate blunt end, 5′ overhangs, or 3′ overhangs. In some examples, one or more nucleotides are removed from the target site during or following cleavage.
Argonaute protein can be endogenously or recombinantly expressed within a cell. Argonaute can be encoded on a chromosome, extrachromosomally, or on a plasmid, synthetic chromosome, or artificial chromosome. Additionally or alternatively, an Argonaute protein can be provided or delivered to the cell as a polypeptide or mRNA encoding the polypeptide. In such examples, polypeptide or mRNA can be delivered through standard mechanisms known in the art, such as through the use of cell permeable peptides, nanoparticles, or viral particles.
Guide DNAs can be provided by genetic or episomal DNA within a cell. In some examples, gDNA are reverse transcribed from RNA or mRNA within a cell. In some examples, gDNAs can be provided or delivered to a cell expressing an Ago protein. Guide DNAs can be provided or delivered concomitantly with an Ago protein or sequentially. Guide DNAs can be chemically synthesized, assembled, or otherwise generated using standard DNA generation techniques known in the art. Guide DNAs can be cleaved, released, or otherwise derived from genomic DNA, episomal DNA molecules, isolated nucleic acid molecules, or any other source of nucleic acid molecules.
In some instances, compositions are provided comprising a nuclease such as an nucleic acid-guided nuclease (e.g., Cas9, Cpf1, MAD2, or MAD7) or a DNA-guided nuclease (e.g., Ago), linked to a chromatin-remodeling enzyme. Without wishing to be bound by theory, a nuclease fusion protein as described herein may provide improved accessibility to regions of highly-structured DNA. Non-limiting examples of chromatin-remodeling enzymes that can be linked to a nucleic-acid guided nuclease may include: histone acetyl transferases (HATs), histone deacetylases (HDACs), histone methyltransferases (HMTs), chromatin remodeling complexes, and transcription activator-like (Tal) effector proteins. Histone deacetylases may include HDAC1, HDAC2, HDAC3, HDAC4, HDACS, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, sirtuin 1, sirtuin 2, sirtuin 3, sirtuin 4, sirtuin 5, sirtuin 6, and sirtuin 7. Histone acetyl transferases may include GCNS, PCAF, Hat1, Elp3, Hpa2, Hpa3, ATF-2, Nut1, Esa1, Sas2, Sas3, Tip60, MOF, MOZ, MORF, HBO1, p300, CBP, SRC-1, ACTR, TIF-2, SRC-3, TAFII250, TFIIIC, Rtt109, and CLOCK. Histone methyltransferases may include ASH1L, DOT1L, EHMT1, EHMT2, EZH1, EZH2, MLL, MLL2, MLL3, MLL4, MLL5, NSD1, PRDM2, SET, SETBP1, SETD1A, SETD1B, SETD2, SETD3, SETD4, SETD5, SETD6, SETD7, SETD8, SETD9, SETDB1, SETDB2, SETMAR, SMYD1, SMYD2, SMYD3, SMYD4, SMYD5, SUV39H1, SUV39H2, SUV420H1, and SUV420H2. Chromatin-remodeling complexes may include SWI/SNF, ISWI, NuRD/Mi-2/CHD, INO80 and SWR1.
In some instances, the nuclease is a wild-type nuclease. In other instances, the nuclease is a chimeric engineered nuclease. Chimeric engineered nucleases as disclosed herein can comprise one or more fragments or domains, and the fragments or domains can be of a nuclease, such as nucleic acid-guided nuclease, orthologs of organisms of genuses, species, or other phylogenetic groups disclosed herein; advantageously the fragments are from nuclease orthologs of different species. A chimeric engineered nuclease can be comprised of fragments or domains from at least two different nucleases. A chimeric engineered nuclease can be comprised of fragments or domains from at least two different species. A chimeric engineered nuclease can be comprised of fragments or domains from at least 2, 3, 4, 5, 6, 7, 8, 9, 10, or more different nucleases or different species. In some cases, more than one fragment or domain from one nuclease or species, wherein the more than one fragment or domain are separated by fragments or domains from a second nuclease or species. In some examples, a chimeric engineered nuclease comprises 2 fragments, each from a different protein or nuclease. In some examples, a chimeric engineered nuclease comprises 3 fragments, each from a different protein or nuclease. In some examples, a chimeric engineered nuclease comprises 4 fragments, each from a different protein or nuclease. In some examples, a chimeric engineered nuclease comprises 5 fragments, each from a different protein or nuclease.
Nuclease fusion proteins can be recombinantly expressed within a cell. A nuclease fusion protein can be encoded on a chromosome, extrachromosomally, or on a plasmid, synthetic chromosome, or artificial chromosome. A nuclease and a chromatin-remodeling enzyme may be engineered separately, and then covalently linked, prior to delivery to a cell. A nuclease fusion protein can be provided or delivered to the cell as a polypeptide or mRNA encoding the polypeptide. In such examples, polypeptide or mRNA can be delivered through standard mechanisms known in the art, such as through the use of cell permeable peptides, nanoparticles, or viral particles.

Cell-Cycle-Dependent Expression of Targeted Nucleases.

In some instances, compositions comprising a cell-cycle-dependent nuclease are provided. A cell-cycle dependent nuclease generally includes a targeted nuclease as described herein linked to an enzyme that leads to degradation of the targeted nuclease during G1 phase of the cell cycle, and expression of the targeted nuclease during G2/M phase of the cell cycle. Such cell-cycle dependent expression may, for example, bias the expression of the nuclease in cells where homology-directed repair (HDR) is most active (e.g., during G2/M phase). In some cases, the nuclease is covalently linked to cell-cycle regulated protein such as one that is actively degraded during G1 phase of the cell cycle and is actively expressed during G2/M phase of the cell cycle. In a non-limiting example, the cell-cycle regulated protein is Geminin. Other non-limiting examples of cell-cycle regulated proteins may include: Cyclin A, Cyclin B, Hsll, Cdc6, Finl, p21 and Skp2.
In some instances, the nuclease is a wild-type nuclease.
In other instances, the nuclease is a engineered nuclease. Engineered nucleases can be non-naturally occurring.
Non-naturally occurring targetable nucleases and non-naturally occurring targetable nuclease systems can address many of these challenges and limitations.
Disclosed herein are non-naturally targetable nuclease systems. Such targetable nuclease systems are engineered to address one or more of the challenges described above and can be referred to as engineered nuclease systems. Engineered nuclease systems can comprise one or more of an engineered nuclease, such as an engineered nucleic acid-guided nuclease, an engineered guide nucleic acid, an engineered polynucleotides encoding said nuclease, or an engineered polynucleotides encoding said guide nucleic acid. Engineered nucleases, engineered guide nucleic acids, and engineered polynucleotides encoding the engineered nuclease or engineered guide nucleic acid are not naturally occurring and are not found in nature. It follows that engineered nuclease systems including one or more of these elements are non-naturally occurring.
Non-limiting examples of types of engineering that can be done to obtain a non-naturally occurring nuclease system are as follows. Engineering can include codon optimization to facilitate expression or improve expression in a host cell, such as a heterologous host cell. Engineering can reduce the size or molecular weight of the nuclease in order to facilitate expression or delivery. Engineering can alter PAM selection in order to change PAM specificity or to broaden the range of recognized PAMs. Engineering can alter, increase, or decrease stability, processivity, specificity, or efficiency of a targetable nuclease system. Engineering can alter, increase, or decrease protein stability. Engineering can alter, increase, or decrease processivity of nucleic acid scanning. Engineering can alter, increase, or decrease target sequence specificity. Engineering can alter, increase, or decrease nuclease activity. Engineering can alter, increase, or decrease editing efficiency. Engineering can alter, increase, or decrease transformation efficiency. Engineering can alter, increase, or decrease nuclease or guide nucleic acid expression.
Examples of non-naturally occurring nucleic acid sequences which are disclosed herein include sequences codon optimized for expression in bacteria, such as E. coli (e.g., SEQ ID NO: 41-60), sequences codon optimized for expression in single cell eukaryotes, such as yeast (e.g., SEQ ID NO: 127-146), sequences codon optimized for expression in multi cell eukaryotes, such as human cells (e.g., SEQ ID NO: 147-166), polynucleotides used for cloning or expression of any sequences disclosed herein (e.g., SEQ ID NO: 61-80), plasmids comprising nucleic acid sequences (e.g., SEQ ID NO: 21-40) operably linked to a heterologous promoter or nuclear localization signal or other heterologous element, proteins generated from engineered or codon optimized nucleic acid sequences (e.g., SEQ ID NO: 1-20), or engineered guide nucleic acids comprising any one of SEQ ID NO: 84-107. Such non-naturally occurring nucleic acid sequences can be amplified, cloned, assembled, synthesized, generated from synthesized oligonucleotides or dNTPs, or otherwise obtained using methods known by those skilled in the art.
Additional examples of non-naturally occurring nucleic acid sequences which are disclosed herein include sequences codon optimized for expression in bacteria, such as E. coli (e.g., SEQ ID NO: 168), sequences codon optimized for expression in single cell eukaryotes, such as yeast (e.g., SEQ ID NO: 169), sequences codon optimized for expression in multi cell eukaryotes, such as human cells (e.g., SEQ ID NO: 170), polynucleotides used for cloning or expression of any sequences disclosed herein (e.g., SEQ ID NO: 171), plasmids comprising nucleic acid sequences (e.g., SEQ ID NO: 167) operably linked to a heterologous promoter or nuclear localization signal or other heterologous element, proteins generated from engineered or codon optimized nucleic acid sequences (e.g., SEQ ID NO: 108-110), or engineered guide nucleic acids compatible with any targetable nuclease disclosed herein. Such non-naturally occurring nucleic acid sequences can be amplified, cloned, assembled, synthesized, generated from synthesized oligonucleotides or dNTPs, or otherwise obtained using methods known by those skilled in the art.
A guide nucleic acid can be DNA. A guide nucleic acid can be RNA. A guide nucleic acid can comprise both DNA and RNA. A guide nucleic acid can comprise modified of non-naturally occurring nucleotides. In cases where the guide nucleic acid comprises RNA, the RNA guide nucleic acid can be encoded by a DNA sequence on a polynucleotide molecule such as a plasmid, linear construct, or editing cassette as disclosed herein.
Nucleic acid-guided nucleases can be compatible with guide nucleic acids that are not found within the nucleases endogenous host. Such orthogonal guide nucleic acids can be determined by empirical testing. Orthogonal guide nucleic acids can come from different bacterial species or be synthetic or otherwise engineered to be non-naturally occurring.
Orthogonal guide nucleic acids that are compatible with a common nucleic acid-guided nuclease can comprise one or more common features. Common features can include sequence outside a pseudoknot region. Common features can include a pseudoknot region (e.g., 172-181). Common features can include a primary sequence or secondary structure.
A guide nucleic acid can be engineered to target a desired target sequence by altering the guide sequence such that the guide sequence is complementary to the target sequence, thereby allowing hybridization between the guide sequence and the target sequence. A guide nucleic acid with an engineered guide sequence can be referred to as an engineered guide nucleic acid. Engineered guide nucleic acids are often non-naturally occurring and are not found in nature.
In other instances, the nuclease is a chimeric nuclease. Chimeric nucleases can be engineered nucleases. Chimeric nucleases as disclosed herein can comprise one or more fragments or domains, and the fragments or domains can be of a nuclease, such as nucleic acid-guided nuclease, orthologs of organisms of genuses, species, or other phylogenetic groups; advantageously the fragments are from nuclease orthologs of different species. A chimeric nuclease can be comprised of fragments or domains from at least two different nucleases. A chimeric nuclease can be comprised of fragments or domains from at least two different species. A chimeric nuclease can be comprised of fragments or domains from at least 2, 3, 4, 5, 6, 7, 8, 9, 10, or more different nucleases or different species. In some cases, more than one fragment or domain from one nuclease or species, wherein the more than one fragment or domain are separated by fragments or domains from a second nuclease or species. In some examples, a chimeric nuclease comprises 2 fragments, each from a different protein or nuclease. In some examples, a chimeric nuclease comprises 3 fragments, each from a different protein or nuclease. In some examples, a chimeric nuclease comprises 4 fragments, each from a different protein or nuclease. In some examples, a chimeric nuclease comprises 5 fragments, each from a different protein or nuclease.

EXAMPLES

Example 1— CREATE-Plasmids and Libraries

FIGS. 1A-C depict an example of an overview of CRISPR EnAbled Trackable genome Engineering (CREATE) design and workflow. FIG. 1A shows an example of the CREATE methodology which allows programmatic genome modifications to be focused on key amino acid residues or promoter targets across the genome. Such libraries thus enable systematic assessment of sequence/activity relationships for a wide variety of genomic targets in parallel. FIG. 1B depicts an example of CREATE cassettes designed to encode both homology arm (HA) and guide RNA (gRNA) sequences to target a specific locus in the E. coli genome. The 100 bp homology arm was designed to introduce a specific codon mutation (target codon) that can be selectively enriched by a synonymous PAM mutation to rescue the sequence from Cas9 cleavage and allow highly efficient mutagenesis. The P1 and P2 sites (black) serve as general priming sites allowing multiplexed amplification, cloning and sequencing of many libraries in parallel. The promoter (J23119, green) is a constitutive promoter that drives expression of the gRNA. Detailed example the HA design for introducing a stop codon at residue 145 in the galK locus is also depicted at the bottom of FIG. 1B. The top sequence shows the wildtype genome sequence with the PAM (CCG; the reverse complement of which is CGG, which is recognized by S. pyogenes Cas9) and target codon (TAT, encoding Y) highlighted. The HA design introduces a “silent scar” at the PAM site (CgG, the reverse complement of which is CCG, which is not recognized by S. pyogenes Cas9) and a single nucleotide TAT>TAA mutation at codon 145 (resulting in a STOP). This design strategy was implemented programmatically for coding regions across the genome. FIG. 1C depicts an overview of an example CREATE workflow. CREATE cassettes are synthesized on a microarray delivered as large oligo pools (10⁴to 10⁶individual library members). Parallel cloning and recombineering allowed processing of these pools into genomic libraries, in some cases in 23 days. Deep sequencing of the CREATE plasmids can be used to track the fitness of thousands of precision mutations genome wide following selection or screening of the mutant libraries.

Example 2— CREATE Plasmid Validation

FIG. 2A-D depicts an example of the effect of Cas9 activity on transformation and editing efficiencies. The galK 120/17 CREATE cassette (120 bp HA and 17 bp PAM/codon spacing) targeting codon 145 in galK gene or a control non-targeting gRNA vector was transformed in cells carrying pSIMS along with dCas9 (e.g. left set of bars in FIG. 2A) or Cas9 (e.g. right set of bars in FIG. 2A) plasmids. The pSIMS plasmid carries lambda red recombination machinery. The cas9 gene was cloned into the pBTBX-2 backbone under the control of a pBAD promoter to allow control of the cleavage activity by addition of arabinose. Transformation efficiencies of each vector are shown with dark grey bars. The total number of recombinant cells (light grey bars) were calculated based on red/white colony screening on MacConkey agar. In cases where white colonies were undetectable by plate based screening we assumed 10⁴editing efficiencies. A 10²fold reduction in transformation efficiency compared to the non-targeting gRNA control was also observed for CREATE cassettes transformed into the Cas9 background.
FIG. 2B depicts an example of the characterization of CREATE cassette HA length and PAM/codon spacing on editing efficiency. All cassettes were designed to introduce a TAA stop at codon 145 in the gene using PAMs at the indicated distance (PAM/codon bottom) from the target codon and variable homology arms lengths (HA, bottom). Dark grey and light grey bars correspond to uninduced or induced expression of Cas9 under the pBAD promoter using 0.2% arabinose. In the majority of cases the editing efficiency appears to be unaffected by induction suggesting that low amounts of Cas9 due to leaky expression are sufficient for high efficiency editing.
FIG. 2C shows example data from sequencing of the genomic loci from CREATE recombineering reactions. The galK cassettes from FIG. 2B are labeled according to the HA length and PAM codon spacing. The other loci shown were cassettes isolated from multiplexed library cloning reactions. The bar plot (FIG. 2C) indicates the number of times each genotype was observed by genomic colony sequencing following recombineering with each CREATE cassette. The + and labels at the bottom indicate the presence or absence of the designed mutation at the two relevant sites in each clone. The circular inset indicates the relative position of each gene on the E. coli genome.
FIG. 2D depicts an example of library coverage from multiplexed cloning of CREATE plasmids. Deep sequencing counts each variant are shown with respect to their position on the genome. The inset shows a histogram of these plasmid counts for the entire library. The distribution follows expected Poisson distribution for low average counts.

Example 3— CREATE-Recording Used to Engineer Trackable Episomal DNA Libraries

FIG. 3A depicts an example of an overview of the method used to generate a trackable episomal DNA library. Transformation of a CREATE recorder plasmid generates modifications of the target DNA at two sites. One edit occurs to the desired target gene (gray) introducing a codon or promoter mutation designed to test specific engineering objectives. The second edit targets a functionally neutral site and introduces a 15 nucleotide barcode (BC, black). By virtue of coupling these libraries on a single CREATE plasmid the target DNA is edited at both sites and each unique barcode can be used to track edits throughout the rest of the plasmid.
FIG. 2B depicts an example of the CREATE barcode design. A degenerate library is constructed from overlapping oligos and cloned in a separate site of the CREATE vector to make a library of CREATE recorder cassettes that can be coupled to the designer editing libraries.
FIG. 2C depicts an exemplary CREATE record mapping strategy. Deep sequencing of both the target DNA (left) and CREATE plasmids allows a simple sequence mapping strategy by allowing each editing cassette to be uniquely assigned by the barcode sequence. This allows the relative fitness of each barcode (and thus edit) to be tracked during selection or screening processes and can be shuttled between different organisms using standard vectors.

Example 4— CREATE-Mediated Editing of Episomal DNA

Methods and compositions disclosed herein were used to mutate a key residue of the cas9 gene used for the CREATE process (e.g. FIG. 4A-4B). A cassette was designed to make an R1335K mutation in the Cas9 protein. This cassette was cloned into a CREATE plasmid and transformed into MG1655 E. coli carrying the pSIM5 and X2-Cas9 vectors. The pSIM5 vector comprises lambda red recombination machinery. The X2-Cas9 vector comprises an arabinose-inducible Cas9 expression cassette. Following three hours recovery in LB supplemented with 0.4% arabinose to induce Cas9 expression, the cells were plated on agar containing antibiotics that maintain selective pressure for replication of both the X2-Cas9 and CREATE plasmids. Colony PCR of random clones revealed the designed edits from the CREATE plasmid were efficiently transferred into the X2-Cas9 plasmid (e.g. FIG. 4B). Of the clones that were sequenced, 100% contained the silent PAM mutation in X2Cas9 and 6/14 (43%) also containing desired coding edit. This is the first demonstration that plasmid based editing using CREATE is robust despite higher copy numbers associated with the plasmid target as compared to previous genome engineering efforts.

Example 5—CREATE-Mediated Editing and Tracking of E. coli Genome-Double Cassette

To test the performance of the recording strategy in a genomic context we tested the ability to edit two distal genomic loci in the E. coli genome (e.g. FIG. 5A). To do so we cloned CREATE recording cassette libraries designed to embed the 15 nucleotide barcodes into the galK locus. After cloning, we isolated a few unique barcodes and cloned a second editing cassette designed to incorporate an F153R mutation in the dihydrofolate reductase (DHFR)/folA gene that was identified by our previous CREATE studies as conferring tolerance to the antibiotic trimethoprim. Genotyping of E. coli strains following transformation of the dual CREATE recording vector according to previously described protocols yielded the data in FIG. 5A. The efficiency of barcoding (100%) was higher than the target genome edit (80-90%), ensuring that edited genomes can be tracked. Of the transformed population we observed >80% of colonies contained the barcode edit in the galK locus as determined by red white colony screening (e.g. FIG. 5B). From the barcoded colonies we found that 85% of the colonies also encoded the DHFR F153R mutation indicating that we have a strong tracking between the barcode and codon edits. FIG. 5B depicts the total number of colonies (CFUs) in duplicate experiments that are edited and/or barcoded. The edited CFUs numbers were calculated by extrapolation of the data in FIG. 5A to the total number of CFUs on the plate. The barcoded CFUs numbers were calculated by counting the number of white colonies in a galK screening (site in which barcode is integrated). These data show that the majority of barcoded colonies contained the designed genomic edit.

Example 6—Plasmid Curing for Combinatorial Engineering

FIG. 6 depicts an example of combinatorial genome engineering and tracking. Three recursive CREATE plasmids are used, each with a gRNA targeting one of the other markers in this series (indicated by T-lines). During each transformation, an edit and barcode are incorporated into the genome and the previous CREATE plasmid is cured. In this way rapid iterative transformations can be performed to construct either a defined combination of mutations or a combinatorial library to search for improved phenotypes. The recording site is compatible with short read sequencing technologies that allow the fitness of combinations to be tracked across a population. Such an approach allows rapid investigation of genetic epistasis and optimization of phenotypes relevant to basic research or for commercial biological applications.
FIG. 3D and FIG. 3E depict another example of combinatorial genome engineering. With each round of engineering, an editing cassette (blue rectangle in FIG. 3D) is incorporated into the target sequence in the genome (blue star) and a recorder cassette (green rectangle in FIG. 3D) is incorporated into a different target sequence of the genome (green dash in middle panel of FIG. 3D). In this example, each recorder sequence comprises a 15 nucleotide barcode. As shown in the right panel of FIG. 3D, the recorder sequences are each inserted adjacent to the last recorder sequence, despite where the editing cassette was inserted. Each recorder cassette can simultaneously delete a PAM site. After completion of each round of engineering, the engineered cells can be selected and then the inserted mutations can be tracked by sequencing the recorder region that comprises all of the inserted recorder cassettes. By sequencing the starting plasmid library, each editing cassette can be linked or associated with one or more unique barcodes within the recorder cassette. Since each recorder cassette corresponds to the associate editing cassette, then the mutations incorporated by the editing cassettes can be tracked or identified by the sequence of the recorder cassette, or the sequence of the barcodes within the recorder cassette. As is demonstrated in FIG. 3E, by sequencing all of the recorder cassettes or barcodes within the recorder cassettes, each of the inserted mutations can be identified and tracked. The inserted recorder sequences can be referred to as a recorder site, recorder array, or barcode array. As a result, after recursive rounds of engineering, sequencing the barcode array or recorder site allows tracking of the history of genomic editing events in the strain. When the recorder cassettes are inserted in order as depicted, for example, in FIG. 3D, then the barcode array or recorder site can identify the order in which the mutations were inserted as well as what the mutation is.

Example 7— Recursive Engineering Using Iterative CREATE-Recording Engineering Events

The example of recursive engineering depicted in FIG. 7A was used for plasmid curing to demonstrate that the design is extremely efficient at eliminating previous vectors (FIG. 7B). Each CREATE plasmid can be positively selected for based on the indicated antibiotics (Trimeth: trimethoprim, Carb: carbenicillin, Tet: tetracycline) and contains a gRNA targeting one of the other antibiotic markers. For example, the reCREATE1 plasmid can be selected for on carbenicillin and encodes a gRNA that will selectively target the trimethoprim resistance gene for destruction. One pass through the carb/tetracycline/trimethoprim antibiotic marker series allows selective incorporation of up to three targeted edits. The recording function would be implemented as illustrated in FIG. 5 , but is omitted here for simplicity.
FIG. 7B depicts an example of data from iterative rounds of CREATE engineering. A serial transformation series began with cells transformed with X2cas9 (kan) and the reCREATE1 vector. The spot plating results indicate that curing is 99.99% effective at each transformation step, ensuring highly efficient engineering in each round of transformation. Simultaneous genome editing and plasmid curing in each transformation step with high efficiencies was achieved by introducing the requisite recording and editing CREATE cassettes into recursive vectors as disclosed herein (e.g. FIG. 7B).

Example 8—CREATE Design and Workflow

An example overview of CRISPR EnAbled Trackable genome Engineering (CREATE) design workflow is depicted in FIGS. 8A-8B. FIG. 8A shows example anatomy of a CREATE cassette designed for protein engineering. Cassettes encode a spacer (red) along with part of a guide RNA (gRNA) sequence and a designer homology arm (HA) that can template homologous recombination at the genomic cut site. For protein engineering purposes the HA is designed to systematically couple mutations to a specified codon or target site (TS, blue) to a nearby synonymous PAM mutation (SPM, red) to rescue the sequence from Cas9 cleavage and allow highly efficient mutagenesis. The priming sites (P1 and P2, black) are designed to allow multiplexed amplification and cloning of specific subpools from massively parallel array based synthesis. A constitutive promoter (green) drives expression of the gRNA. FIG. 8A further shows a detailed example of HA design for introducing a stop codon at residue 145 in the galK locus. The top sequence shows is of the wt genome with the PAM and TS codon highlighted. The translation sequences are shown to illustrate that the resulting mutant contains a single nonsynonymous mutation at the target site. FIG. 8B shows an example overview of the CREATE workflow. CREATE oligos are synthesized on a microarray and delivered as large pools (10⁴-10⁶individual library members). These cassettes are amplified and cloned in multiplex with the ability to subpool designs. After introduction of the CREATE plasmids into cells expressing Cas9 mutations are transferred to the genome with high efficiencies. Measurement of the frequency of each plasmid before (fi, t1) and after selection (fi, t2) by deep sequencing provides enrichment scores (Ei) for each CREATE cassette. These scores allow rapid identification of adaptive variants at up to single nucleotide or amino acid resolution for thousands loci in parallel.

Example 9—CREATE Design Validation

FIG. 9A depicts an example of the effects of Cas9 activity on transformation and editing efficiencies were measured using no a cassette with a spacer and 120 bp HA targeted to the galK (galK_Y145*_120/17) The total transformants (TT white) produced by this CREATE vector are shown in white and the total number of recombinants (TR) in dark blue. TR is calculated as the product of the editing efficiency and Tt. Asterisks indicate experiments in which recombinants could not be observed by plate based screening. FIG. 9B shows an example of characterization of CREATE cassette HA length and PAM/codon spacing on editing efficiency. All cassettes were designed to introduce a TAA stop at codon 145 in the gene using PAMs at the indicated distance (PAM/codon bottom) from the target codon and variable homology arms lengths (HA, bottom). White and blue bars correspond to uninduced or induced expression of Cas9 under the pBAD promoter using 0.2% arabinose. In the majority of cases the editing efficiency appears to be unaffected by induction suggesting that low amounts of Cas9 due to leaky expression are sufficient for high efficiency editing. FIG. 9C depicts an example of determination of editing efficiency for oligo derived cassettes by sequencing of the genomic loci. The galK_Y145*_120/17 cassette from FIGS. 9A and 9B is shown in white for reference. The bar plot indicates the number of times each genotype was observed by genomic colony sequencing following recombineering with each CREATE cassette. The circular inset indicates the relative position of each gene on the E. coli genome. FIG. 9D depicts distance between SPM and the TS (as exemplified in FIG. 8A) is strongly correlated with editing efficiency (correct edits/total sequences sampled). The galK cassettes with 44 and 59 bp in FIG. 9B were omitted from this analysis. The depicted error bars are derived from N=3 independent replicates of the indicated experiment.

Example 10—Scanning Saturation Mutagenesis of an Essential Chromosomal Gene

FIG. 10A-10C depict an example where CREATE was used to generate a full scanning saturation mutagenesis library of the folA gene for identification of mutations that can confer resistance to TMP. The count weighted average enrichment score from two trials of selection is plotted as a function of residue position (right). Cassettes encoding nonsynonymous mutations are shown in gray, and those encoding synonymous mutations in black. Cassettes with enrichment scores greater than 1.8 are highlighted in red and mutations that affect previously reported sites are labeled for reference. The dashed lines indicate enrichment values that are significantly different (p<0.05) from the synonymous dataset as determined by bootstrapping of the confidence intervals. These values are shown as a histogram for reference (middle). Mutations that appear to significantly impact DHFR resistance are highlighted as red spheres to the far right. FIGS. 10D-10F depict example growth analysis of wt (left) F153W (middle) and F153R (right) variants in the indicated range of TMP concentrations (shown right).

Example 11—Reconstruction of ALE Mutation Set and Forward Engineering of Thermotolerant Genotypes

FIG. 11A depicts example genomic plots of enrichment scores for CREATE libraries grown at 42.2° C. in minimal media conditions. The innermost plot illustrates the counts of the plasmid library before selection with labels for the top 20 representatives. The outer ring shows the fitness of pooled library variants after growth in minimal media at elevated temperature (42.2° C.). The bars are colored according to log 2 enrichment. Blue bars represent detrimental mutations, red bars represent significantly enriched mutations and gray bars indicate mutations that appear neutral in this assay. The 20 most enriched variants are labeled for reference and labels corresponding to ALE-derived variants are colored red. FIG. 11B shows a histogram of enrichment scores of all library variants (gray), ALE-derived mutants (red) and synonymous mutants (black) under 42.2° C. growth conditions. The dotted gray line indicates significant enrichment scores compared to the synonymous population. The histograms are normalized as a fraction of the total number of variants passing the counting threshold (number indicated in parentheses). Note that 231 of 251 unique nonsynonmous ALE cassettes sampled by this experiment appear to provide significant growth benefits. FIG. 11C depicts enrichment of mutations based on mutational distance from wt. Mutations that require 2 and 3 nucleotide (nt) transitions are exceedingly rare or absent in ALE approaches however we note that the two most enriched clones from the pooled library selection (targeting the Crp regulator) require two nucleotide substitutions and are highlighted at the far right.

Example 12—Genome Scale Mapping of Amino Acid Substitutions for the Study of Antibiotic Resistance and Tolerance

FIG. 12A depicts example genomic plots of enrichment (log 2) of library variants in the presence of erythromycin (outer) and rifampicin (middle). The innermost plot illustrates the count distribution of the input plasmids for reference. Coloring and labeling are as in FIG. 11A-11C. FIG. 12B depicts CREATE mutation mapping at the individual amino acid level. CREATE cassettes that introduce bulky side chains to amino acids 1572, S531 and L533 (red) of the RNA polymerase β subunit (rpoB) are highly enriched in the presence of rifampicin from genome wide targeting libraries. FIG. 11C depicts a zoomed in region of the MarA transcription factor bound to its cognate DNA target is shown for reference (PDB ID 1BL0). The wt Q89 residue protrudes away from the DNA binding interface due to unfavorable steric and electrostatic interactions between this side chain and the DNA. The Q89N substitution identified by selection introduces a H-donor and shortens the side chain such that productive H-bonding can occur between this residue and the DNA backbone. Such an interaction likely favors stronger DNA binding and induction of downstream resistance genes. FIG. 12D depicts enrichment plot of genome wide targeting libraries with 10 g/L acetate or 2 g/L furfural respectively. Coloring is the same as in FIG. 11A. FIG. 12E depicts CREATE mapping at a gene level reveals trends at the gene level. Strong enrichment fis metA and fadR targeting mutations in acetate suggests important roles for these genes in acetate tolerance, as depicted in FIG. 12F, same as in the furfural selections depicted in FIG. 12E.

Example 13—CREATE-Enabled Flexible Design Strategies

Illustration of example designs compatible with CREATE strategy are depicted in FIGS. 13A-13D. FIG. 13A shows protein engineering applications a silent codon approach is taken (top, see also FIG. 8A-8B). This mutation strategy allows targeted mutagenesis of key protein regions to alter features such as DNA binding, protein-protein interactions, catalysis, or allosteric regulation. Above an illustration of a DNA binding saturation mutagenesis library designed for the global transcription factor Fis designed for this study is illustrated. FIG. 13B shows promoter mutations PAM sites in proximity to a specified transcription start site (TSS) can be disrupted through nucleotide replacement or integration cassettes. To simplify this design procedure used in this study consensus CAP or UP elements were designed for integration at a fixed location relative to the TSS without taking into account possible effects of these mutations may have on proximal genes. FIG. 13C shows an example cassette design for mutagenizing a ribosome binding site (RBS). FIG. 13D depicts an example of a simple deletion design. Points a and b are included to illustrate distance between two sites at the gene deletion locus. In all cases cassette designs disrupt a targeted PAM to allow selective enrichment of the designed mutant.

Example 14—Engineering the Lycopene Pathway

FIGS. 14A-14B depict edits made the DMAPP pathway in E. coli which is the precursor to lycopene. Edits were made to the ORF's for 11 genes. Eight edits were designed to improve activity and 3 edits were designed to reduce activity of competitive enzymes. Approximately 10,000 variants within the lycopene pathway were constructed and screened.

Example 15—Cas9 Editing Efficiency Controls

FIG. 15 depicts Cas9 editing control experiments. The CREATE galK_120/17 off cassette (relevant edits shown in red at bottom) was transformed into different backgrounds to assess the efficiency of homologous recombination between the CREATE plasmid and the target genome. Red colonies represent unedited (wt) genomic variants and white colonies represent edited variants. Transformation into cells containing only pSIM5 or pSIM5/X2 and dCas9 plasmids exhibited no detectable recombination as indicated by the lack of white colonies. In the presence of active Cas9 (X2-Cas9 far right) we observe high efficiency editing (>80%), indicating the requirements for dsDNA cleavage to achieve high efficiency editing and library coverage.

Example 16—Toxicity of gRNA dsDNA Cleavage in E. coli

FIGS. 16A-16C depict experiments testing the toxicity of generating double strand breaks in E. coli. The toxicity of a single gRNA cut in E. coli as observed in control experiments with a gRNA targeting galK (spacer sequence TTAACTTTGCGTAACAACGC (SEQ ID NO: 182)) or folA (spacer sequence GTAATTTTGTATAGAATTTA (SEQ ID NO: 183)). In the absence of a repair template we observe strong killing from the gRNA. Rescue efficiencies of 10³-10⁴are observed upon co-transformation of a single stranded donor oligo indicating the need for a homologous repair template to alleviate this toxicity. b) Toxicity of multiple CREATE edits. The targeted sites are illustrated graphically on the left and at the bottom of the bar graph. A non-targeting gRNA control was used to estimate transformation efficiency based on no edits (far left, no target sites). A CREATE cassette targeting either folA (green) or galK (red) or a combination of the two. Note the multiplicative toxicity in E. coli of having additional gRNAs expressed from the same plasmid. In this scenario there is homologous repair for each site suggesting that off-target gRNA cleavage would be highly lethal. These data suggest that off target cleavage by a CREATE cassette would be selectively removed from the population early in the library construction phase.
FIGS. 16D-16E depicts data from another such cell survival assay. The editing cassette contained a F153R mutation, which leads to temperature sensitivity of the folA gene. The recorder cassette contained a 15 nucleotide barcode designed to disrupt the galK gene, which allows screening of colonies on MacConkey agar plates. In this example, generating two cuts decreased cell survival compared to generating zero or one cut.
FIG. 16F depicts data from a transformation and survival assay comparing a low copy number plasmid (Ec23) expressing Cas9 and a high copy number plasmid (MG) expressing Cas9. Different vectors with distinct editing cassettes were used to target different gene target sites (folA, lacZ, xylA, and rhaA). The recorder cassettes were designed to target different sequences within the galK gene, either site S1, S2, or S3. The recursive vector used had a different vector backbone compared to the others and is part of a 3-vector system designed for iterative engineering that cures the cell of the previous round vector. The data indicates that lower Cas9 expression (Ec23 vector) increases survival and/or transformation efficiency. The decreased Cas9 expression increased transformation efficiency by orders of magnitude in cells undergoing two genomic cuts (editing cassette and recording cassette).
FIG. 16G shows the correlation between editing efficiency and recording efficiency in cells transformed with the low copy number plasmid (Ec23) expressing Cas9 and the high copy number plasmid (MG) expressing Cas9. Editing and recording efficiencies were similar for high (MG) and lower (Ec23) expression of cas9. Ec23 yielded more colonies and had better survival (as shown in FIG. 16E), while maintaining a high efficiency of dual editing (editing cassette and recorder cassette incorporation).

Example 17—CREATE Strategy for Gene Deletion

FIG. 17A-D depict an example CREATE strategy for gene deletion. FIG. 17A depicts an example cassette design for deleting 100 bp from the galK ORF. The HA is designed to recombine with regions of homology with the designated spacing, with each 50 bp side of the CREATE HA designed to recombine at the designated site (blue). The PAM/spacer location (red) is proximal to one of the homology arms and is deleted during recombination, allowing selectable enrichment of the deleted segment. FIG. 17B depicts electrophoresis of chromosomal PCR amplicons from clones recombineered with this cassette. FIG. 17C depicts design for 700 bp deletion as in a). FIG. 17D depicts colony PCR of 700 bp deletion cassettes as in FIG. 17B). The asterisks in FIGS. 17B and 17D indicate colonies that appear to have the designed deletion. Note that some clones appear to have bands pertaining to both wt and deletion sizes indicating that chromosome segregation in some of the colonies is incomplete when plated 3 hrs post recombineering.

Example 18—Editing Efficiency Controls by Cotransformation of gRNA and Linear dsDNA Cassettes

FIG. 18 depicts effect of PAM distance on editing efficiency using linear dsDNA PCR amplicons and co-transformation with a gRNA. On the left is an illustration of the experiments using PCR amplicons containing a dual (TAATAA) stop codon on one side (asterisk) and a PAM mutation just downstream of the galK gene (gray box) on the other end were co-transformed with a gRNA targeting the downstream galK PAM site. The primers were designed such that the mutations were 40 nt from the end of the amplicon to ensure enough homology for recombination. Data was obtained from these experiments by red/white colony screening. A linear fit to the data is shown at the bottom. Cassettes in which only the PAM mutation is present were included as assay controls were observed to have very low rates of GalK inactivation. These experiments were performed in a BW25113 strain of E. coli in which the mutS gene was knocked out to allow high efficiency editing with double stranded DNA templates. This approach in MG1655 did not achieve high efficiency editing due to the active mutS allele.

Example 19—Library Cloning Analysis and Statistics

FIG. 19A depicts reads from an example plasmid library following cloning are shown according to the number of total mismatches between the read and the target design sequence. The majority of plasmids are matches to the correct design. However, there are a large number of 4 base pair indel/mismatch mutants that were observed in this cloned population. FIG. 19B depicts a plot of the mutation profile for the plasmid pool as a function of cassette position. An increase in the mutation frequency is observed near the center of the homology arm (HA) indicating a small error bias in the sequencing or synthesis of this region. We suspect that this is due to the presence of sequences complementary to the spacer element in the gRNA. FIG. 19C depicts a histogram of the distances between the PAM and codon for the CREATE cassettes designed in this study. Large majority (>95%) were within the design constraints tested in FIG. 9A-9D. The small fraction that are beyond 60 bp were made in cases where there was no synonymous PAM mutation within closer proximity. FIG. 19D depicts library coverage from multiplexed cloning of CREATE plasmids. Deep sequencing counts each variant are shown with respect to their position on the genome. The inset shows a histogram of the number of variants having the indicated plasmid counts in the cloned libraries.

Example 20—Precision of CREATE Cassette Tracking of Recombineered Populations

FIG. 20A depicts a correlation plot of CREATE cassette read frequencies in the plasmid population prior to Cas9 exposure (x-axis) and after 3 hours post transformation into a Cas9 background. FIG. 20B depicts a correlation plot between replicate recombineering reactions following overnight recovery. The gray lines indicate the line of perfect correlation for reference. R2 and p values were calculated from a linear fit to the data using the Python SciPy statistics package. A counting threshold of 5 for each replicate experiment was applied to the data to filter out noise from each data set.

Example 21—Growth Characteristics of folA Mutations in M9 Minimal Media

FIG. 21 depicts growth characteristics of folA mutations in M9 minimal media. While F153R appears to maintain normal growth characteristics the growth rate of the F153W mutation is significantly slower under these conditions, suggesting that these two amino acid substitutions at the same site have very different effects on organismal fitness presumably due to different changes invoked in the stability/dynamics of this protein.

Example 22—Enrichment Profiles for folA CREATE Cassettes in Minimal Media

FIG. 22 depicts enrichment profiles for folA CREATE cassettes in minimal media. Cassettes that encode synonymous HA are shown in black and non-synonymous cassettes in gray, the dashed lines indicate enrichment scores with p<0.05 significance compared to the synonymous population mean as estimated from a bootstrap analysis. The enrichment score observed for each mutant cassette at each position in the protein sequence is shown to the left and a histogram of these enrichment scores as a fraction of the total variants to the right. The two populations appear to be largely similar. Conserved residues that are highly deleterious are shown in blue for reference.

Example 23—Validation of Newly Identified acrB Mutations for Improved Solvent and Antibiotic Tolerance

FIG. 23A depicts on the left a global overview of AcrB efflux pump. Substrates enter the pump through the openings in the periplasmic space and are extruded via the AcrB/AcrA/TolC complex across the outer membrane and into the extracellular space. Library targeted residues are highlighted by blue spheres for reference and the red dot indicates the region where many of the enriched variants clustered. On the right is a blow up of the loop-helix motif abutting the central funnel where enriched mutations in isobutanol were identified (red and teal spheres), presumably affecting solute transport from the periplasmic space. Mutants targeting the T60 position (teal spheres) was also enriched in the presence of erythromycin. FIG. 23B depicts confirmation of N70D and D73L mutations for tolerance to isobutanol. The N70D mutation in particular appears to improve the final OD to a significant degree. Reconstructed strains were measured for final OD in capped 1.5 mL eppendorf tubes following 48 hours incubation. Error bars are derived from N=3 trials and p-values derived from a one-tailed T-test. FIG. 23C depicts improved growth of the AcrB T60N mutant was observed in inhibitory concentrations of erythromycin (200 μg/mL) and isobutanol (1.2%) in shaking 96 well plate, indicating that this mutation may enhance the efflux activity of this pump towards many compounds. For these experiments CREATE cassette designs were individually synthesized, cloned and sequence verified before recombineering into E. coli MG1655 to reconstruct the mutations and the genomic modifications were sequence verified by colony PCR to confirm the genotype-phenotype association.

Example 24—Benefits of Rational Mutagenesis for Sampling Novel Adaptive Genotypes

FIGS. 24A-24D depict the number of variants detected in CREATE experiments involving 500 μg/mL rifampicin (FIG. 24A), 500 μg/mL erythromycin (FIG. 24B), 10 g/L acetate (FIG. 24C), and 2 g/L furfural (FIG. 24D). While naturally evolving systems or error-prone PCR are highly biased towards sampling single nucleotide polymorphisms (e.g. 1 nt mutations, red) these histograms illustrate the potential advantages for rational design approaches that can identify rare or inaccessible mutations (2 and 3 nt, green and blue respectively). For example, the highest fitness solutions appear to be biased toward these rare mutations in rifampicin, erythromycin and furfural selections to varying degrees. These results indicate that procedures such as CREATE should allow more rapid and thorough analysis of fitness improving mutations, in much the same way that computational approaches are being used to improve directed evolution for protein engineering.

Example 25—Reconstruction of Mutations Identified by Erythromycin Selection

FIG. 25 depicts reconstructed strains grown in 0.5 mL in capped 1.5 mL eppendorf tubes following 48 hours incubation in the presence of 200 μg/mL erythromycin and final OD measurements assessed. Error bars are derived from N=3 trials. A one tailed T-test was performed on each set of measurements to determine p-values indicated for significance of growth benefit.

Example 26—Validation of Crp S28P Mutation for Furfural or Thermal Tolerance

FIG. 26A depicts a crystal structure of the Crp regulatory protein with variants identified by furfural selection highlighted in red (PDB ID 3N4M). A number of the CREATE designs targeting residues near the cyclic-AMP binding site (aa. 28-30, 65) of this regulator were highly enriched in minimal media selections for furfural or thermal tolerance suggesting that these mutations may enhance E. coli growth in minimal media under a variety of stress conditions. FIG. 26B depicts validation the Crp S28P mutant identified in 2 g/L furfural selections in M9 media. This mutant was reconstructed as described for AcrB T60S in Example 23.

Example 27—Genome-Scale Sequence to Activity Relationship Mapping at Single Nucleotide Resolution

Advances in DNA synthesis and sequencing have motivated increasingly complex efforts to rationally program genomic modifications on laboratory timescales. Realization of such efforts requires strategies that span the design-build-test forward-engineering cycle by not only precisely and efficiently generating large numbers of mutant designs but also by mapping the effects of these mutations at similar throughputs. CRISPR EnAbled Trackable genome Engineering (CREATE) couples highly efficient CRISPR editing with massively parallel oligomer synthesis to enable trackable precision editing on a genome wide scale. This can be accomplished using synthetic cassettes that link a targeting guide RNA with rationally programmable homologous repair cassettes that can be systematically designed to edit loci across a genome and track their phenotypic effects. We demonstrated the flexibility and ease of use of CREATE for genome engineering by parallel mapping of sequence-activity relationships for applications ranging from site saturation mutagenesis, rational protein engineering, complete residue substitution libraries and reconstruction of prior adaptive laboratory evolution experiments.
Validation of CREATE Cassette Design
In order to realize our engineering objectives we took into account a number of key design considerations to both maximize the editing efficiency as well as distill a complex design process into an easily executable workflow. For example, each CREATE cassette is designed to include both a targeting guide RNA (gRNA) and a homology arm (HA) that introduces rational mutations at the chromosomal cleavage site (e.g. FIG. 8A). The HA encodes both the genomic edit of interest coupled to a synonymous PAM mutation that is designed to abrogate Cas9 cleavage after repair (e.g. FIG. 8B). This arrangement not only ensures that the desired edit can be selectively enriched to high levels by Cas9 but also that the sequences required to guide cleavage and HR are covalently coupled during synthesis and thus delivered simultaneously to the same cell during transformation. The high efficiency editing of CRISPR based selection in E. coli should also ensure a strong correlation between the CREATE plasmid and genomic sequences and allow the plasmid sequence to serve as a trans-acting barcode or proxy for the genomic edit (e.g. FIG. 8C). Assuming that changes in the plasmid frequency under different selective pressures are correlated to their associated genomic edit thereby allows the impact of precise genomic modifications at many loci to be monitored in parallel using a simple downstream sequencing approach to map enriched genotypes on a population scale, analogous to previous genomic tracking methodologies.
To test this concept we first performed control experiments using a CREATE cassette designed to inactivate the galK gene by introducing a single point mutation to convert codon 145 from TAT to a TAA stop codon (e.g. FIG. 8B) using a 120 bp HA. The editing efficiency of this cassette using Cas9 and the nuclease deficient dCas9 control was evaluated using a red/white colony screening assay (e.g. FIG. 8A-B, FIG. 15A-15C). These experiments also indicated that HR between a circular double stranded plasmid and the chromosome is strongly dependent on the Cas9 cleavage as recombination is not observed in the absence of the active enzyme (e.g. FIG. 15A-15D). This is in contrast to single stranded recombineering approaches in which oligonucleotides anneal with high efficiency at the lagging strand of the replication fork. Cas9 also adversely impacts the overall transformation efficiency due to toxicity of dsDNA cleavage in E. coli (e.g. FIG. 9A-9D). This toxicity is further exacerbated when performing CREATE at two sites simultaneously in the same cell (e.g. FIG. 16A-16E); which when combined with the absence of an effective non-homologous end joining pathway strongly supports the fact that off target editing events should be rare within a recombineered library. Additionally, toxicity limits the size of library construction and coverage, however we note that the observed 10⁴-10⁵variants/μg DNA (e.g. FIG. 9A) is on a scale compatible with current oligo synthesis capabilities (10^4-5oligos per order). Thus, we anticipated that using the CREATE synthetic oligo design, we would be able to simultaneously generate ˜10⁵or more designer mutations at any location in the genome and precisely map such mutations onto a targeted phenotype.
To further characterize how changes in the CREATE cassette design influence the editing efficiency we varied the HA length (80-120 bp) and the distance between the PAM-codon/TS (17-59 bp) (e.g. FIG. 9B). Induction of Cas9 revealed that all of these cassette variants can support high efficiency HR. High efficiency conversion is also observed in the absence of Cas9 induction indicating that low level expression of Cas9, due to a leaky inducible promoter, is sufficient to drive cleavage and HR (e.g. FIG. 9B). To verify that the edits matched our intended design we sequenced the chromosome of randomly chosen clones and found that 71% (27/38) contained a perfect match to the CREATE design, while 26% (10/38) contained only the PAM edit and the remaining 3% (1/38) appeared to be wt escapers. As an additional test of design flexibility performed similar experiments using deletion cassettes that that introduce different sized deletions (e.g. FIG. 17A-17D) and observed similar efficiencies (>70%) indicating that the same design automation and tracking capabilities should readily extend to a variety of design objectives (e.g. FIG. 13A-13D).
High-Throughput Design and Multiplexed Library Construction
To scale the CREATE process for genome-wide applications we developed a custom software to automate cassette design that takes into account the above mentioned criteria to systematically identify a PAM sequence nearest to a target site (TS) of interest and modify it to create a synonymous PAM mutation. This design software is part of a suite of web-based design tools that can be implemented for E. coli and is under further development for other organisms as well as an expanded set of CRISPR-Cas systems. This software platform enables high-throughput rational design of genomic libraries in a format that is compatible with parallelized array based oligo synthesis and simple homology based cloning methods that can be performed in batch for library construction (e.g. FIG. 8B).
Using this design software we generated a total of 52,356 CREATE cassettes for a range of applications where sequence to activity mapping by traditional methods would be time-consuming and prohibitively expensive. Briefly, the library designs included: 1) a complete saturation of the folA gene to map the entire mutational landscape of an essential gene in its chromosomal context 2) saturation mutagenesis of functional residues in 35 global regulators, efflux pumps and metabolic enzymes implicated in a wide range of tolerance and production phenotypes in E. coli 3) a reconstruction of the complete set of nonsynonymous mutations identified by a recent adaptive laboratory evolution (ALE) study of thermotolerance, and 4) promoter engineering libraries designed to incorporate UP elements or CAP binding elements at transcription start sites annotated in RegulonDB (e.g. FIG. 13A-13D).
The pooled oligo libraries were amplified and cloned in parallel and a subset of single variants were isolated to further characterize editing efficiency at different loci (e.g. FIG. 9C). Amplification and sequencing of the genomic loci after transformation with the CREATE plasmids revealed editing efficiencies of 70% on average (106 of 144 clones sampled at seven different loci), with a range of 30% for the metA V20L cassette to 100% for the rpoH_V179H cassette. Interestingly, the differences in editing efficiency for each cassette were highly correlated with the distance between the PAM and target codon (e.g. FIG. 9D), a feature that also appears to affect the ability of linear DNA templates to effectively introduce targeted mutations (e.g. FIG. 18A-18B). This relationship suggests that subsequent CREATE designs should readily increase editing efficiency by optimizing PAM selection criteria. We also note that differences in editing efficiency may reflect detrimental effects of some mutations on organismal fitness (metA is considered an essential gene in most media conditions), and that there may be an upper bound on the number of mutations that can be observed for a particular protein. Finally, these data were obtained outside of any specific selective or screening steps that enrich for chromosomal mutants of interest, and as such demonstrate the ability of this approach to construct mutational libraries.
To further characterize the fidelity of the multiplexed synthesis and cloning procedures we performed deep sequencing on the pooled libraries (e.g. FIG. 19A-D). From 594,998 total reads of the cloned CREATE cassette libraries, 550,152 (92%) passed quality filtering and produced hits against the design database. Of these we observed a perfect match for 34,291 (65%) of the possible unique variants and note that many cassettes that were missing in this initial pool were observed in later selections, suggesting that at the cloning stage we can readily cover the majority of the intended design space. In depth analysis of these reads revealed that 46% of the reads passing quality filter were exact matches to their intended design, with the remainder containing 1-4 bp indels or mismatches, primarily in the HA region near the designed mutation site (e.g. FIG. 19A). The mutational bias in this region suggests that the repetitive spacer elements in the HA and gRNA portions of the cassette may form secondary structures that adversely affect sequencing or synthesis (e.g. FIG. 19B). We note that these variant designs are easily identified via the CREATE plasmid-barcoding strategy, and that in some cases it may be desired to have this added diversity in the generated library. We also observed significant (p<0.05) correlation between variant frequencies from the cloned pools and after overnight recovery following recombineering, as well as between replicate recombineering experiments (e.g. FIG. 20A-20B). These results suggest that well represented variants should be readily tracked by our methodology with a precision similar to previous CRISPR based saturation mutagenesis procedures performed at a single loci.
CREATE Based Protein Engineering
To test the robustness of the CREATE methodology for protein engineering at a single gene level we performed deep-scanning mutagenesis of the essential folA gene. This gene encodes the dihydrofolate reductase (DHFR) enzyme responsible for the production of tetrahydrofolate and the biosynthesis of pyrimidines, purines and nucleic acids. DHFR is also the primary target of the antibiotic trimethoprim (TMP) and other antifolates that are used as antibiotics or chemotherapeutics. The wealth of structural and biochemical data DHFR function and antibiotic resistance make it an ideal model for validation of the approach.
A CREATE library designed to saturate every codon from 2-158 of the DHFR enzyme was recombineered into E. coli MG1655 and allowed to recover overnight. Following recovery ˜10⁹cells (1 mL saturated culture) was transferred into media containing inhibitory TMP concentrations and allowed to grow for 48 hours. The resulting plasmid populations were then sequenced to assess our ability to capture information at the level of single amino acid substitutions that can confer TMP resistance (e.g. FIG. 10A-10B). Bootstrapped confidence intervals for mutational effect were derived using the enrichment data of the 158 synonymous mutations included in this experiment (e.g. FIG. 10A-10B). Using this criteria, we observed significant (P<0.05) levels of enrichment for 74 substitutions (2.3% of the design space) covering 49 aa positions in the protein. Although this degree of mutational flexibility of an essential enzyme may seem counterintuitive, it supports previous conclusions that this enzyme has not reached its evolutionary optimum and that many mutations that can improve TMP tolerance through enhancement of the endogenous enzymatic activity or alteration of the dynamic folding landscape of this enzyme.
These results also support the fact that we probe more deeply into the mutation space of improved fitness variants using rational mutagenesis strategies. For example, we observed 7 significantly enriched substitutions at position F153 (e.g. FIG. 10A-10B), none of which have been previously identified by error-prone PCR and adaptive laboratory evolution (ALE). To validate these specific mutations, we reconstructed F153R and F153W variants, which had not been previously reported in the literature and spanned a large range of the measured enrichment scale at this position (e.g. FIG. 10D-10F). We confirmed that the highly enriched F153R mutant grows rapidly under a large range of TMP concentrations while the F153W mutant demonstrates growth only at the moderate TMP concentration used in the selection, consistent with their respective enrichment scores (e.g. FIG. 10A-10F). Moreover, 6 of the 7 mutations we identified using CREATE require two nucleotide changes to convert the wt TTT codon to one of the observed amino acids (I: 1 nt,W: 2 nt,D: 2 nt,R: 2 nt,P: 2 nt,M: 2 nt,H: 2 nt). The F153R and F153W mutations also appear to impact the native enzyme activity in distinct ways (e.g. FIG. 21 ), implying that these substitutions may confer tolerance by altering the enzymatic cycle of this enzyme in distinct manners.
In addition to mapping substitutions that confer TMP resistance, we also attempted to identify substitutions that affect the native activity of DHFR. To do so, we compared the frequencies of each plasmid variant after overnight growth in M9 (e.g. FIG. 22A-22C). In this case, we observed similar overall enrichment profiles for both synonymous and nonsynonymous mutation sets, with very few mutations observed to have significant impact on growth. This unexpected result suggests a need for greater sequencing depth and/or alternate selection strategies to assign high confidence to low fitness variants.
As a separate validation of protein engineering applications, we generated a 4,240 variant library targeting the AcrB multidrug efflux pump in E. coli (e.g. FIG. 23A-23F). This protein acts as a proton exchange pump that exports a wide variety of chemicals including antibiotics, chemical mutagens, and short chain alcohols that are being pursued as next generation biofuels and motivating numerous engineering efforts. The library was designed to target the interior chamber, the exit funnel that channels substrates towards the outer-membrane component of the AcrB/AcrA/TolC complex, and key regions of the transmembrane domain where mutations conferring tolerance to isobutanol and longer chain alcohols have been identified (e.g. FIG. 23A-23C). We then constructed the AcrB CREATE library identically as for the FolA library and grew the library in the presence of 1.2% isobutanol. Sequencing identified multiple mutations to the loop-helix motif adjacent to the central efflux funnel that were significantly enriched, suggesting this substructure may provide a novel target for engineering enhanced efflux activity. Reconstruction of the AcrB N70D and D73L mutations also confirmed the ability of these mutations to enhance overall growth in the presence of this solvent stress (e.g. FIG. 23D).
Parallel Evaluation of Genotype Fitness from Large Scale Adaptation Studies
We next sought to expand our efforts from the single protein scale and validate the use of CREATE at the genome-scale. To do so we chose to reconstruct and map mutations resulting from a prior adaptive laboratory evolution study of E. coli thermal tolerance. ALE has been used extensively as a tool to study the bacterial adaptation in response to a broad range of environmental stressors. However, in the majority of cases the genome undergoes multiple mutations making it difficult to assess the contribution of each mutation to the phenotype in question. Here, we designed and constructed a CREATE library to include all 645 nonsynonymous mutants from the Tenaillon et al ALE experiment and then subjected this library to growth selection in minimal media at 42.2° C. To assess any possible effects that could arise from the synonymous PAM mutation we included redundancy in the design of this library such that each target codon was coupled to two different PAM mutations to provide a 4 fold design redundancy for each nonsynonymous mutation. For calibration purposes the ALE library was pooled with the protein targeting libraries to allow for relative enrichment comparisons from the non-ALE derived libraries as a benchmark (e.g. FIG. 11A-11C). Of the more than 50,000 cassettes in this experiment we observed 405 cassettes from the ALE derived library above the minimal counting threshold, pertaining to 252 unique variants (e.g. FIG. 11B). Of these 346 cassettes (encoding 231 nonsynonymous changes) were significantly enriched compared with the synonymous controls (e.g. FIG. 11B), suggesting that 92% (231/252) of the mutations sampled confer significant selective growth advantages as individual chromosomal mutations, consistent with their fixation during adaptive growth. Additionally we found that 141 mutations from the additional CREATE libraries were also significantly enriched, with 86 of these targeting residues in or around the cAMP binding site of Crp, a central regulator of carbon metabolism. The identification of such a large number of Crp mutants is highly suggestive of a role for Crp in thermal-tolerance in agreement with previous findings.
For each mutant we also calculated the number of mutations required to convert the wt codon to each of the other 19 amino acids (e.g. FIG. 11C). As with folA, we found that highly impactful mutations, such as the crp S28P and L30Y mutations, require more than a single nucleotide substitution and would therefore be inaccessible or exceedingly rare in naturally evolving systems under laboratory timescales. In fact, this seemed to be a recurrent theme across many of the selections we performed (e.g. FIG. 24A-24D) highlighting again the value of synthetic DNA driven search strategies for genomic engineering applications.
High-Throughput Mapping of Selectable Precision Edits on a Genome Wide Scale
To further validate the method for genome-scale mapping and exploration we challenged genome wide targeting libraries with antibiotics or solvents relevant to bioproduction (e.g. FIG. 12A-12F). In the case of selections performed with rifampicin, an antibiotic that inhibits transcription by the RNA polymerase (e.g. FIG. 12A, inner circle) we observed a number of enriched variants that highlighted the robustness of the CREATE approach for atomic resolution mapping. For example, 10 of the top 50 hits identified mutations to residues 1572, L533 and S531 of the RNA polymerase (3 subunit (encoded by rpoB) including variants that form part of the rifampicin binding site (e.g. FIG. 12B). In 6 of the 7 enriched variants the data suggest that a bulky substitution is necessary to sterically hinder 7 rifampicin binding. In addition to the (3-subunit mutations the rifampicin selections enriched a number mutations to the MarA transcriptional activator, whose over-expression due to marR knockout is a well studied aspect of multiple antibiotic resistance (MAR) phenotypes in E. coli. In the DNA bound crystal structure of MarA, Q89 is positioned near the DNA backbone but pointed into solution due to a steric clash between other possible rotamers and nearest phosphate group on the DNA backbone (e.g. FIG. 12C). Modeling of the MarA Q89N and Q89D mutations identified by this selection suggests that shortening the side chain by a single carbon unit may enable new protein-DNA H-bonding interactions and thereby improve the overall MAR induction response.
To compare these results to an antibiotic that interferes with translation we performed another round of selections in the presence of erythromycin (e.g. outer circle FIG. 12A). The enrichment profiles from this selection again highlighted loci previously implicated in resistance to this antibiotic. For example, we observed strong enrichment of 4 different mutations to the AcrB efflux pump which acts as the primary exporter of this drug from the periplasmic space (e.g. FIG. 12A). Interestingly, one of the variants (AcrB T60N) appears at the same residue identified from isobutanol selections (e.g. FIG. 23A-23F). As with the other mutations, reconstruction validated that at least two of these mutations (e.g. T60N in FIG. 23E-23F and D73L in FIG. 25 ) can significantly improve tolerance to both erythromycin as well as isobutanol isobutanol, further supporting the idea that this motif may provide a useful engineering target for broad range of tolerance phenotypes. In addition to AcrB we also observed enrichment of multiple soxR and rpoS mutants, both of which have been previously implicated in stress tolerance and general antibiotic resistance phenotypes. In total, we observed 136 of the 341 significantly enriched mutations (40%) were identified within the RpoB, MarA, MarR, SoxR, AcrB, or dxs proteins, each of which has extensive prior validation as antibiotic resistance genes.
Finally, we performed selections using furfural or acetate, common components of cellulosic hydrolysate that inhibit bacterial growth under industrial fermentation conditions and are thus the target of many strain engineering efforts (e.g. FIG. 12D-12F). In the presence of high acetate concentrations (10 g/L, e.g. inner plot FIG. 12D) the top 100 ranking mutations were predominated by cassettes targeting the fis, fadR, rho and fnr genes respectively (e.g. FIG. 12E). The Fis, Fnr and FadR regulators are all involved transcriptional regulation of the primary acetate utilization gene acs, and implicated in the so-called “acetate-switch” which allows the cell to effectively scavenge acetate. Knockout of these regulators leads to constitutive expression of the acetate utilization pathways and improved acetate growth phenotypes suggesting that the mutations identified in this study (e.g. FIG. 12E-12F) likely inhibit these regulatory functions by destabilizing their respective protein targets.
In contrast to the weak acid tolerance of acetate, the enrichment profiles obtained the presence of growth inhibiting concentrations of furfural (2 g/L) were significantly different with the most frequently observed mutations targeting the oxidative stress response regulator rpoS (e.g. FIG. 12F). Furfural growth inhibition is thought to occur through depletion of cellular NADPH pools, an important cofactor in the prevention of oxidative stress and anabolic pathways for cell growth. In line with our findings, previous studies of RpoS have demonstrated that inactive alleles are favored in such nutrient depleted scenarios. Interestingly, we also observed some of the same mutations in crp that were observed in the 42.2° C. selections (e.g. FIGS. 11A and 11C) and upon reconstruction confirmed that the Crp S28P mutant can substantially improve growth in the presence of furfural (e.g. FIG. 26A-26B). We also found that this selection uniquely enriched for variants of the PntA transhydrogenase, a membrane bound transhydrogenase that transfers hydride ions from NADH to NADP+ to maintain sufficient pools for anabolism. A mutation to I258A in close proximity to the substrate binding cleft may therefore impart enhanced NADPH production.
Collectively, these selections validate the CREATE strategy by demonstrating the ability to map known associations as well as highlight power of this method for rapid mapping of novel mutations to traits of interest. It is also important to note that in contrast to the most other functional genomics technologies that mainly identify loss of function mutations, the ability to perform such broad scale scanning mutagenesis opens the door for more general genomic searches that can also identify novel gain of function mutations.
In this work we have demonstrated that CREATE allows parallel mapping of tens of thousands of amino acid and promoter mutations in a single experiment. The construction, selection, and mapping of >50,000 genome-wide mutations (e.g. FIGS. 11A-11C and 12A-12F) can in some examples be accomplished in 1-2 weeks by a single researcher, offering orders of magnitude improvement in economics, throughput, and target scale over the current state of the art methods in synthetic biology. Importantly, the ability to track the enrichment of library variants allows multiplex sequence to activity mapping by a simple PCR based workflow using just a single set of primers as opposed to more complicated downstream sequencing approaches that are limited to a few dozen loci. In addition, the ability to map the effects of single nucleotide or amino acid level variation in coding regions or promoters allows CREATE to address a considerably more diverse set of design objectives than previous high-throughput genomic technologies such as trackable multiplexed recombineering (TRMR) or Tn-seq approaches that are limited to gene resolution analysis. Such capabilities enable new paradigms for deciphering gene function and engineering cellular traits including workflows in which iterative rounds of CREATE could be implemented to perform design-driven genome engineering and address a broad range of ambitions.
Notably, as a further distinction from prior approaches, the high efficiency mutagenesis (e.g. FIG. 9A-9D) reported in this work was not only an order of magnitude improved but was also achieved in a wild type MG1655 strain in which all of the native DNA repair pathways are intact. The majority of previously reported recombineering efforts in E. coli have used single-stranded oligo engineering which requires deletion of the mismatch repair genes or chemically modified oligonucleotides to achieve mutagenesis at 1-30% efficiency. The combination of plasmid based homologous recombination substrates and Cas9 dsDNA cleavage appears to circumvent these requirements (e.g. FIG. 13A-13D and FIG. 9A-9D), eliminating the need for specialized genetic modifications outside of the Cas9 and k-RED genes to perform efficient editing and tracking on a population scale (e.g. FIG. 9A-9D). This fact alongside the broad utility of CRISPR editing suggests that the CREATE approach will readily port to a wide range of microorganisms such as Saccharomyces cerevisiae and other recombinogenic bacteria for which high-efficiency transformation protocols are available. The CREATE strategy should also be compatible with a wide range of CRISPR/Cas systems using similar automation approaches to design and tracking. Extension of this methodology to higher eukaryotes however will require the development of strategies to overcome non-homologous end-joining as well as alternative tracking systems that can stably replicate.
The CREATE strategy provides a streamlined approach for sequence to activity mapping and directed evolution by integrating multiplexed oligo synthesis, CRISPR-CAS editing, and high-throughput sequencing.

Example 28—Genome-Scale Sequence to Activity Relationship Mapping at Single Nucleotide Resolution, Additional Examples

Possible Effects of Inconsistent Mapping of Plasmid Barcode to Genomic Edit
We note that the initial CREATE library included designs that we would expect to have low confidence mapping between the plasmid barcode and the genomic edit (as explained primarily by distance between the PAM and target mutation in the CREATE cassette, see FIG. 2 d ). We describe below the various scenarios that may arise in the fraction of cases where the plasmid tracking may lead to erroneous conclusions regarding a genomic variant. A few things to note in evaluating these scenarios include i) the plasmid cassette should have minimal or no functional influence relative to the genomic edit, ii) the genomic loci will only be either the WT sequence or the sequence from the editing cassette that we obtain via sequencing, and iii) offsite editing is highly unlikely given the toxicity of CRISPR-Cas editing of multiple sites (e.g. FIG. 16A-16E) or when performed in the absence of an added editing-repair template. Finally, we note that the use of replicate experiments and deeper sequencing can also address these issues.
Tracking of High Fitness Variants (Positive Enrichment Tracking)
In cases where there is a strong selective advantage for the genomic modification (and thus the associated plasmid) we will only observe cells with the edit in the chromosome post selection. Thus, this is almost always a true positive particularly when selection times are short, thus limiting the possibility of random mutations due to replication error sweeping the population. While this phenomenon may lead to a quantitative underestimation of the true fitness of a mutation due to an enrichment profile that represents the convolution of modified and wt fitness, it will not produce false positives. Moreover, the use of replicated experiments and/or longer selections can also address this potential issue and eliminate erroneous conclusions regarding a mutations impact on fitness.
Tracking of Low Fitness Variants (Negative Enrichment Tracking)
In cases where the encoded mutation has a negative fitness contribution but is linked to a PAM only or unmodified chromosome we would incorrectly overestimate the fitness of the mutant and assume that it is closer to wt, especially for longer selection times (e.g. see FIG. 22A-22C). However, any deep sequencing approach must deal with similar limitations due to the lack of information regarding such mutations following selection and the problems associated with counting statistics in these scenarios. Moreover, we would note that this scenario is only relevant to the subset of truly negative fitness mutants (which should be 10-20% based on historic directed evolution and ALE data) within the unedited fraction (˜30%) and that remain in the unedited fraction in multiple replicate transformations. In other words, it is a small percentage (4-5%) scenario that can be detected and/or addressed through replicate transformations where one would observe inconsistencies in the particular mutant showing up occasionally with WT fitness.
Incomplete Coverage
In cases where a variant is not present in the initial population (due to both low transformation efficiency and low editing efficiency) a couple of scenarios could arise. As implied by the points above, if the mutation is beneficial one could falsely conclude that it does not confer a fitness advantage, and if it is truly deleterious it also could be incorrectly assigned a neutral fitness score. This appears to be encountered sometimes in this work and impacts both the error associated with replicate measurements and our ability to distinguish low fitness variants from a synonymous control. However, our ability to identify beneficial mutants is robust despite these issues as evidenced by our ability to readily identify novel and previously validated mutations. Strategies to address this by overcoming Cas9 toxicity and improving recombineering efficiencies hold promise to largely eliminate such problems. Furthermore, increasing the number of replicates, increasing sequencing depth, and/or improving the library coverage by performing larger scale transformation also can help to address these issues.
Off Target gRNA Cleavage
Off target gRNA cleavage should be rare in E. coli due to the relatively small size of its genome (4 Mb), and thus lack of (non-targeted) regions of homology to the CREATE cassette. Moreover, the toxicity of gRNAs in the presence of Cas9 (e.g. FIG. 9A) ensures that cells survival is compromised in E. coli due to dsDNA breaks. Each additional cut introduced into E. coli appears to incur multiplicative toxicity effects, even when homologous repair templates are provided for each cut site (e.g. FIG. 16A-16E). This toxicity effect would be further exacerbated by the absence of a repair template to guide HR (e.g. FIG. 16A-16E), as would be the case for an off-target cleavage event from a single gRNA targeting two sites but containing only a single HA.
Random Off Target Mutagenesis (Evolution)
The probability that a CREATE variant is strongly enriched due to an off target mutation even is highly improbable due to 2 factors: 1) the toxicity effect for the reasons stated above and 2) the low mutation rates of MG1655 or other mutation repair proficient strains compared with the mutagenesis rates of CREATE, particularly in multiple replicates of selection. We also have validated that we can transfer the plasmid pool back into a naive parental background and rapidly verify the enrichment of fitness improving CREATE plasmids from the initial population. Like replicate data, this allows us to decouple each CREATE plasmid from the potential of background mutations that would interfere with our analysis. These factors simplify the assumptions made during our analysis, the validity of which is supported both by externally and internally validated genotypes that were identified during this work.
Possible Effects of Synonymous Mutations
Synonymous mutations (e.g. in the PAM region) can confer unexpected effects on phenotype. We have controlled for this in a number of manners. In every experiment we included an internal control that consists of a library of synonymous mutations ( 1/20 at each codon or 5% of total input), each of which samples different PAM and codon combinations and thus give us an idea of the range of possible effects we may have on a gene by measuring the enrichment profile of many synonymous changes. Using this population as a control we can accurately identify significant fitness changes at the resolution of single amino acids as the work suggests. We can also control for this effect by utilizing redundant sampling approaches where a site is coupled to multiple PAM mutations similar to what was done for the ALE study described herein.
CREATE Library Design Considerations
A variety of design principles were implemented in the gene targeting libraries described in some work disclosed herein. For example, the folA library (3140 cassettes) was designed to be an unbiased, exploratory library for full single site saturation mutagenesis and sequence activity. However, for the majority of the genes we sought to maximize the probability of interesting genotypes by choosing to focus the diversity of sites most likely to have a functional impact on the targeted protein (e.g. DNA binding sites, active sites, regions identified as mutational hotspots by previous selections). The sites that were included in these library designs were selected based on information deposited in databases including Ecocyc (biocyc.org/), Uniprot (uniprot.org/), and the PDB (rcsb.org/pdb) as well as relevant literature citations that identified residues or regions of interest using directed evolution approaches. The Uniprot and Ecocyc databases provide manually curated sequence features that indicate mutational effects and important domains of each protein. In cases where there was enough structural information to model ligand or DNA binding sites the relevant crystal structures were loaded into Pymol and manual residue selections were made and exported as numerical lists. For promoter libraries we took into account the spacing of these sites relative to the transcription start site and the canonical recognition sequence of either the CRP binding site (AAATGTGAtctagaTCACATTT located between −72 and −40 relative to the transcription start site) or the UP element (AAAATTTTTTTTCAAAAGTA (SEQ ID NO: 185) −60 from the transcription start site) that directly recruit the alpha subunit of the RNA polymerase. These sequences were designed to integrate at these positions relative to the publicly available transcriptional start site annotations in RegulonDB using a variation of the automated CREATE design software designed for protein targeting (e.g. FIG. 13A-13D). These cassettes were made with the intent of assessing the effects of gene dosage and regulation on fitness. Finally, we designed a library to reconstruct all of the 645 non-synonymous mutations targeting 197 genes that were identified by a comprehensive ALE experiment in which the complete genomes of 115 isolates were sequenced after a year of adaptation to growth at elevated temperature (e.g. 42.2° C.). In all, we designed 52,356 oligomers, with 48,080 intended to saturate 2404 codon positions across 35 genes, 2,550 oligos were made for regenerating the ALE mutations, 379 UP promoter mutants and 772 CAP promoter mutations in a manner that would allow simultaneous sequence to activity relationship mapping.
Cassette Design and Automation Principles
Based on the control experiments with galK (e.g. FIG. 9A-9D) and current maximal commercial synthesis length constraints (200 bp from Agilent) we developed a general design for each CREATE cassette (e.g. FIG. 8A-8B).
Design of the CREATE cassettes was automated using custom Python scripts. The basic algorithm takes a gene sequence, a list of target residues, and a list of codons as inputs. The gene sequence is searched for all available PAM sites with the corresponding spacer sequence. This list is then sorted according to relative proximity to the targeted codon position. For each PAM site in the initial list the algorithm checks for synonymous mutations that can be made in-frame that also directly disrupt the PAM site, in the event that this condition is met the algorithm proceeds to making the prescribed codon change and designing the full CREATE cassette with the accompanying spacer and iterates for each input codon and position respectively. For each PAM mutation, all possible synonymous codon substitutions are checked before proceeding to the next PAM site. For the codon saturation libraries in this study we chose the most frequent codons (genscript.com/cgi-bin/tools/codon_freq_table) for each designed amino acid substitution according to the E. coli usage statistics. The script can be run rapidly on a laptop computer and was used to generate the full design of these libraries in <10 minutes. The algorithm used in this study was designed to make the most conservative mutations possible by sometimes using only the PAM as the selectable mutation marker.
Plasmids
The X2-cas9 broad host range vector was constructed by amplifying the cas9 gene from genomic S. pyogenes DNA into the pBTBX2 backbone (Lucigen). A vector map and sequence of this vector and the galK_Y145*_120/17 CREATE cassette are provided at the following locations: benchling.com/s/3c941j/edit; benchling.com/s/xRBDwcMy/edit.
The editing experiments performed in some of this work employed the X2-cas9 vector in combination with the pSIM5 vector (redrecombineering.ncifcrf.gov/strains-plasmids.html) to achieve the reported efficiencies.
Recombineering of CREATE Libraries
Genomic libraries were prepared by transforming CREATE plasmid libraries into a wildtype E. coli MG1655 strain carrying the temperature sensitive pSIM5 plasmid (lambda RED) and a broad host range plasmid containing an inducible cas9 gene from cloned from S. pyogenes genomic DNA into the pBTBX-2 backbone (X2cas9, e.g. FIG. 15A-15D). pSIM5 was induced for 15 min at 42° C. followed by chilling on ice for 15 min. The cells were washed 3 times with ⅕ the initial culture volume of ddH2O (e.g. 10 mL washes for 50 mL culture). Following electroporation the cells were recovered in LB+0.4% arabinose to induce Cas9. The cells were recovered 1-2 hrs before spot plating to determine library coverage and transferred to a 10× volume for overnight recovery in LB+0.4% arabinose+50 μg/mL kanamycin+100 μg/mL carbenicillin. Saturated overnight cultures were pelleted and resuspended in 5 mL of LB. 1 mL was used to make glycerol stocks and the other 1 mL washed with the appropriate selection media before proceeding with selection.
For the control experiments with galK we used CREATE cassettes designed to convert Y145 (TAT) into a stop codon (TAA) with a single point mutation at this position and a second point mutation to make a synonymous mutation that abolishes the targeted PAM site (e.g. FIG. 8B and FIG. 13A-13D). Editing efficiencies (e.g. FIG. 13A-13D and FIG. 9A-9B) were estimated using red/white plate based screening on 1% galactose supplemented MacConkey agar as previously described.
Selection Procedures
Following overnight recovery, the cells were harvested by pelleting and resuspension in fresh selection media. All selections were performed in shake flask and inoculated at an initial OD600 of 0.1. Three serial dilutions (48-96 hrs depending on growth rates in the target condition) were carried out for each selection by transferring 1/100th the media volume after the cultures reached stationary phase. The 42° C. selections were performed in M9 media+0.2% glucose to mimic low carbon availability from the initial adaptation. Antibiotic selections were carried out in LB+500 μg/mL rifampicin or erythromycin to ensure stringent selection. The solvent selections were performed in M9+0.4% glucose and either 10 g/L acetate (unbuffered) or 2 g/L furfural. Selections were harvested by pelleting 1 mL of the final culture and the cell pellet was boiled in 100 μL TE buffer to preserve both the plasmid and the genomic DNA for further desired analyses.
Library Preparation and Sequencing
Custom Illumina compatible primers were designed to allow a single amplification step from the CREATE plasmid and assignment of experimental reads using barcodes. The CREATE cassettes were amplified directly from the plasmid sequences of boiled cell lysates using 20 cycles of PCR with the Phusion (NEB) polymerase using 60° C. annealing and 1:30 minute extension times. As in the cloning procedure a minimal number of PCR cycles was maintained to prevent accumulation of mutations and recombined CREATE cassettes that were observed when an excessive number of PCR cycles was implemented (e.g. >25-30). Amplified fragments were verified and quantified by 1% agarose gel electrophoresis and pooled according to the desired read depth for each sample. The pooled library was cleaned using Qiaquick PCR cleanup kit and processed for NGS using standard Illumina preparation kits. The Illumina sequencing and sample preparation were performed with the primers.
Preprocessing of High-Throughput Sequencing and Count Generation
Paired-end Illumina sequencing reads were sorted according to the golay barcode index with allowance of up to 3 mismatches then merged using the usearch-fastq_merge algorithm. Sorted reads were then matched against the database of designed CREATE cassettes using the usearch global algorithm at an identity threshold of 90% allowing up to 60 possible hits for each read. The resulting hits were further sorted according to percent identity and read assignment was made using the best matching CREATE cassette design at a final cutoff 98% identity to the initial design. It should be noted that this read assignment strategy attempts to identify correlations between the designed genotypes and may therefore miss other important features that arise due to mutations that could occur during the experimental procedure. This approach was taken both to simplify data analysis as well as evaluate the ‘forward’ design and annotation procedure and it's ability to accurately identify meaningful genetic phenomena.
Data Analysis and Fitness Calculation
Enrichment scores (or absolute fitness scores) were calculated as the log 2 enrichment score using the following equation:
$W = \log 2 (\frac{F_{x, f}}{F_{x, i}}),$
where F_x,fis the frequency of cassette X at the final time point and F_x,iis the initial frequency of cassette X and W is the absolute fitness of each variant. Frequencies were determined by dividing the read counts for each variant by the total experimental counts including those that were lost to filtering. Each selection was performed in duplicate and the count weighted average of the two measurements was used to infer the average fitness score of each mutation as follows:
$W_{avg} = \frac{Σ_{i = 1}^{N} {counts}_{i} * W_{i}}{Σ_{i = 1}^{N} {counts}_{i}}$
These scores were used to rank and assess the fitness contributions of each mutation under the various selection pressures investigated. For all selections we took average absolute fitness scores for all of the synonymous mutants as a composite measure of the average growth rate. Absolute enrichment scores were considered significant if the mutant enrichment was at least +/−2*σ (e.g. p=0.05 assuming a normal distribution) of the wild-type value. We performed two replicates of each selection reported in this study to derive these figures and applied a cutoff threshold of 10 across the replicate experiments for inclusion in each analysis.
For every codon targeted our designs also included a synonymous variant to provide an internal experimental control. Thus 5% of the protein targeting cassettes encoded synonymous mutations that allow us to estimate confidence intervals for mutation effects using custom Python bootstrapping scripts. The enrichment data for each experiment was resampled with replacement 20000 to obtain 95% confidence interval estimations that were used to infer statistical significance of enrichment scores for each analysis presented in the manuscript.
Mutant Reconstructions and Growth Measurements
The AcrB T60N and Crp S28P and FolA F153R/W CREATE cassettes were ordered as separate gblocks from IDT, cloned and sequence verified. Each cassette was transformed into MG1655 and colony screened to identify a clone with the designed genomic edit. These strains (e.g. FIG. 21 and FIG. 22A-22C) were then subjected to the growth conditions from the pooled library selection as indicated. The growth curves were taken in triplicate for each condition in 100 μL in a 96 well plate reader set to measure absorbance at 600 nm. The plate was covered and water added to empty wells to reduce evaporation during the growth.
Software and Figure Generation
Circle plots were generated using Circos v0.67. Plots were generated in Python 2.7 using the matplotlib plotting libraries and figures were made using Adobe Illustrator CS5. Entropy scores for the FolA (FIG. 10A) were determined using the ProDy Python package and the Pfam accession PF00186 representative proteome alignment RP35.
Figures of the protein libraries and high fitness mutations were made using The PyMol Molecular Graphics System, Schrodinger, LLC. The following are the proteins and PDBs used in the figure generation: AcrB (3W9H, 4K7Q, 3AOC), Fis (3JR9), Ihf (1IHF), RNA polymerase (4KMU, 4IGC), Crp (3N4M), MarA (1BLO), and SoxR (2ZHG).

Example 29: Testing Edit-Barcode Correlation

A strain expressing a low copy number plasmid (Ec23) which is a Cas9-pSIM5 dual vector, was tested using different gene editing cassettes (lacZ, xylA, and rhaA) and recorder cassettes with different barcodes and insertion sites (galK site 1, galK site 2, and galK site 3) (Summarized in FIG. 27A). The possible outcomes are depicted in FIG. 27B. Pre-selection, all combinations of edit/barcode/WT are possible. After selection, edits cells could be enriched whether they are barcoded or not in this experimental design.
The transformations were plated on selective media that allowed for enrichment of cells containing the gene edits. 30 colonies from each combination transformation were sequenced to determine if they contained the desired barcode.
FIG. 27C shows the results from the sequencing data. Two of the edit/barcode combinations were found in 100% of the tested colonies (30/30 colonies), and the other edit/barcode combination transformation was found in approximately 97% of tested colonies (29/30 colonies). The single colony that was not properly engineered contained the gene edit, but not the barcode.
Overall, 89 out of 90 tested colonies has the designed gene edit and barcode.

Example 30: Selectable Recording

When a barcode is not selected for, it allows for enrichment of non-barcoded cells even if the corresponding gene edit is incorporated and selected for. FIG. 28 depicts an example strategy for selecting for the recording event (e.g., incorporation of the barcode by the recorder cassette), in addition to selecting for the editing cassette incorporation, thereby increasing the efficiency of recovering cells that have been both edited and barcoded.
As depicted in FIG. 28 , sequences S0, S1, S2, etc. are designed to be targeted by the guide RNA associated with the recorder cassette of the next round. In the depicted example, in the first round of engineering, a PAM mutation, a barcode, S1 site, and regulatory elementary necessary to turn on a selectable marker are incorporated into the S0 site in the target region. This turns on the TetR selectable marker and allows for enrichment of barcoded mutants variants with the S1 site that have the first round PAM site deleted. In the second round of engineering, a new recorder cassette comprising a second PAM mutation, a second barcode, a S2 site, and a mutation that turns off the selectable marker is incorporated into the S1 site from the previous round. This allows for counter-selection of variants that have incorporated the second barcode and S2 site. The subsequent rounds continue to flip the selectable marker between an on and off state and using selection or counter-selection respectively to enrich the desired variants. The recorder cassette from each round is designed to incorporate into a unique sequence (e.g., S0, S1, etc.) that was incorporated in the previous round. This ensures that the last round of barcoding was successful so that all desired engineering steps are contained in the final product. The incorporation of PAM mutations at each step also helps ensure that the desired barcoded variants are selected for since cells having the unmodified PAM sequences will be killed as they can't escape CRISPR enzyme cleavage.
This strategy uses multiple methods to increase the efficiency of isolating desired variants that contain all of the engineered edits from each round of engineering. The PAM mutation, selectable marker switch, and unique landing site incorporated in each round separately increase efficiency and together increase efficiency as well. These tools allow for selection of each recording round and allow design of highly active recording guide RNAs. An array of equally spaced (or not equally spaced, depending on the design) barcodes is generated and facilitates downstream analysis such as sequencing the barcode array to determine which corresponding edits are incorporated throughout the genome.
FIG. 29 depicts an experimental design to test the selectable recorder strategy described above. A plasmid (pREC1) containing an editing cassette and a recorder cassette was transformed into cells. The editing cassette either contained a non-targeting editing cassette, or a mutation that incorporated a mutation (not TS) or a temperature sensitive mutation (TS) into a target gene. The recorder cassette was designed to incorporate into the S0 site in the target gene that originally had the tetR selectable marker turned off. The recorder cassette also contained a PAM mutation that deleted the S0 PAM site, first barcode (BC1), a unique S1 site for the subsequent engineering round recording cassette to incorporate into, and a corrective mutation that will turn on the TetR selectable marker. A guide RNA on the recorder cassette that targets a PAM site in the S0 site (S0-gRNA) allows a CRISPR enzyme, in this case Cas9, to cleave the S0 site. The recorder cassette recombines into the cleaved S0 site. The PAM mutation is incorporated, which means the S0-gRNA can no longer target the S0 site, thereby killing WT cells and enriching for cells that received the barcode. The TetR selectable marker was also turned on, allowing further selection of the barcoded variant.
The data in FIGS. 30A and 30B show the results from the experiment described above and depicted in FIG. 29 . Of the Tet Resistant colonies that were recovered from the transformation and engineering round, 16 were sequence and determined to all contain the designed barcode (FIG. 30A). FIG. 30B shows that the control cells that did not contain the recorder target site (non-target) did not survive the presence of Tet, while cells that contained the target site were successfully barcoded as evidences by the turning on of TetR, allowing cells to be selected on Tet containing media. The Tet resistant colonies were confirmed at the genomic site to have TetR gene turned on. These data showed that selectable recording was successful.

Example 31: Expression of MAD Nucleases

Wild-type nucleic acid sequences for MAD1-MAD20 include SEQ ID NOs 21-40, respectively. These MAD nucleases were codon optimized for expression in E. coli and the codon optimized sequences are listed as SEQ ID NO: 41-60, respectively (summarized in Table 2). Codon optimized MAD1-MAD20 were cloned into an expression construct comprising a constitutive or inducible promoter (e.g., T7 promoter SEQ ID NO: 83, or pBAD promoter SEQ ID NO: 81 or SEQ ID NO: 82) and an optional 6×-His tag (SEQ ID NO: 186). The generated MAD1-MAD20 expression constructs are provided as SEQ ID NOs: 61-80, respectively.

TABLE 2

	WT	Codon
MAD	nucleic	optimized
nu-	acid	nucleic acid	Amino acid	Expression
clease	sequence	sequence	sequence	constructs

MAD1	SEQ ID	SEQ ID NO: 41	SEQ ID NO: 1	SEQ ID NO: 61
	NO: 21
MAD2	SEQ ID	SEQ ID NO: 42	SEQ ID NO: 2	SEQ ID NO: 62
	NO: 22
MAD3	SEQ ID	SEQ ID NO: 43	SEQ ID NO: 3	SEQ ID NO: 63
	NO: 23
MAD4	SEQ ID	SEQ ID NO: 44	SEQ ID NO: 4	SEQ ID NO: 64
	NO: 24
MAD5	SEQ ID	SEQ ID NO: 45	SEQ ID NO: 5	SEQ ID NO: 65
	NO: 25
MAD6	SEQ ID	SEQ ID NO: 46	SEQ ID NO: 6	SEQ ID NO: 66
	NO: 26
MAD7	SEQ ID	SEQ ID NO: 47	SEQ ID NO: 7	SEQ ID NO: 67
	NO: 27
MAD8	SEQ ID	SEQ ID NO: 48	SEQ ID NO: 8	SEQ ID NO: 68
	NO: 28
MAD9	SEQ ID	SEQ ID NO: 49	SEQ ID NO: 9	SEQ ID NO: 69
	NO: 29
MAD10	SEQ ID	SEQ ID NO: 50	SEQ ID NO: 10	SEQ ID NO: 70
	NO: 30
MAD11	SEQ ID	SEQ ID NO: 51	SEQ ID NO: 11	SEQ ID NO: 71
	NO: 31
MAD12	SEQ ID	SEQ ID NO: 52	SEQ ID NO: 12	SEQ ID NO: 72
	NO: 32
MAD13	SEQ ID	SEQ ID NO: 53	SEQ ID NO: 13	SEQ ID NO: 73
	NO: 33
MAD14	SEQ ID	SEQ ID NO: 54	SEQ ID NO: 14	SEQ ID NO: 74
	NO: 34
MAD15	SEQ ID	SEQ ID NO: 55	SEQ ID NO: 15	SEQ ID NO: 75
	NO: 35
MAD16	SEQ ID	SEQ ID NO: 56	SEQ ID NO: 16	SEQ ID NO: 76
	NO: 36
MAD17	SEQ ID	SEQ ID NO: 57	SEQ ID NO: 17	SEQ ID NO: 77
	NO: 37
MAD18	SEQ ID	SEQ ID NO: 58	SEQ ID NO: 18	SEQ ID NO: 78
	NO: 38
MAD19	SEQ ID	SEQ ID NO: 59	SEQ ID NO: 19	SEQ ID NO: 79
	NO: 39
MAD20	SEQ ID	SEQ ID NO: 60	SEQ ID NO: 20	SEQ ID NO: 80
	NO: 40

Example 32: MAD2 and MAD7 Nucleases

MAD2 and MAD7 nucleases are nucleic acid-guided nuclease that can be used in the methods disclosed herein. Nucleases Mad2 (SEQ ID NO: 2) and Mad 7 (SEQ ID NO: 7) were cloned and transformed into cells. Editing cassettes designed to mutate a target site in a galK gene were designed with mutations, which allowed for white/red screening of successfully editing colonies. The editing cassettes also encoded a guide nucleic acid designed to target galK. The editing cassettes were transformed into E. coli cells expressing MAD2, MAD7, or Cas9. FIG. 31A shows the editing efficiency of Mad2 and Mad7 compared to Cas9 (SEQ ID NO: 110). FIG. 31B shows the transformation efficiency as evidenced by cell survival rates. In this example, the guide nucleic acid used with MAD2 and MAD7 comprised a scaffold-12 sequence and a guide sequence targeting galK. The guide nucleic acid used with Cas9 comprised a sequence compatible with the S. pyogenes Cas9.
FIG. 32 and Table 3 show more examples of gene editing using the MAD2 nuclease. In this experiment, different guide nucleic acid sequences were tested. The guide sequence of the guide nucleic acids targeted the galK gene as described above. The scaffold sequence of the guide nucleic acids were one of various sequences tested as indicated. Guide nucleic acids with scaffold-5, scaffold-10, scaffold-11, and scaffold-12 were able to form functional complexes with MAD2.
FIG. 33 and Table 4 show more examples of gene editing using the MAD7 nuclease. In this experiment, different guide nucleic acid sequences were tested. The guide sequence of the guide nucleic acids targeted the galK gene as described above. The scaffold sequence of the guide nucleic acids were one of various sequences tested as indicated. Guide nucleic acids with scaffold-10, scaffold-11, and scaffold-12 (e.g., FIG. 31A) were able to form functional complexes with MAD7. Amino acid sequences are provided in Table 2 and scaffolding sequences are provided in Table 3 and Table 4. Table 3 and Table 4 also provided the designed mutations in the editing cassettes that were used to mutate the galK target gene.
Further details and characterization of MAD2, MAD7, and other MAD nucleases are described in U.S. application Ser. No. 15/631,989, filed Jun. 23, 2017, and U.S. application Ser. No. 15/632,001, filed Jun. 23, 2017, each of which are incorporated herein in their entirety.

TABLE 3

	Nucleic		Editing
	acid-guided	Guide nucleic acid	sequence	Target
#	nuclease	scaffold sequence	mutation	gene

1	MAD2	Scaffold-12; SEQ ID NO: 95	N89KpnI	galK
2	MAD2	Scaffold-10; SEQ ID NO: 93	L80**	galK
3	MAD2	Scaffold-5; SEQ ID NO: 88	L80**	galK
4	MAD2	Scaffold-12; SEQ ID NO: 95	D70KpnI	galK
5	MAD2	Scaffold-12; SEQ ID NO: 95	Y145**	galK
6	MAD2	Scaffold-11; SEQ ID NO: 94	Y145**	galK
7	MAD2	Scaffold-10; SEQ ID NO: 93	Y145**	galK
8	MAD2	Scaffold-12; SEQ ID NO: 95	L10KpnI	galK
9	MAD2	Scaffold-11; SEQ ID NO: 94	L80**	galK
10	SpCas9	S. pyogenese gRNA	Y145**	galK
11	MAD2	Scaffold-2; SEQ ID NO: 85	Y145**	galK
12	MAD2	Scaffold-4; SEQ ID NO: 87	Y145**	galK
13	MAD2	Scaffold-1; SEQ ID NO: 84	L80**	galK
14	MAD2	Scaffold-13; SEQ ID NO: 96	Y145**	galK

TABLE 4

	Nucleic		Editing
	acid-guided	Guide nucleic acid	sequence	Target
#	nuclease	scaffold sequence	mutation	gene

1	MAD7	Scaffold-1; SEQ ID NO: 84	L80**	galK
2	MAD7	Scaffold-2; SEQ ID NO: 85	Y145**	galK
3	MAD7	Scaffold-4; SEQ ID NO: 87	Y145**	galK
4	MAD7	Scaffold-10; SEQ ID NO: 93	Y145**	galK
5	MAD7	Scaffold-11; SEQ ID NO: 95	L80**	galK

While preferred embodiments of the present invention have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the invention. It should be understood that various alternatives to the embodiments of the invention described herein may be employed in practicing the invention. It is intended that the following claims define the scope of the invention and that methods and structures within the scope of these claims and their equivalents be covered thereby.

SEQUENCE LISTING

TABLE 5

SEQ
ID
NO:	Sequence

SEQ	MGKMYYLGLDIGTNSVGYAVTDPSYHLLKFKGEPMWGAHVFAAGNQSAERRSFRTSRRRL
ID	DRRQQRVKLVQEIFAPVISPIDPRFFIRLHESALWRDDVAETDKHIFFNDPTYTDKEYYS
NO:	DYPTIHHLIVDLMESSEKHDPRLVYLAVAWLVAHRGHFLNEVDKDNIGDVLSFDAFYPEF
1	LAFLSDNGVSPWVCESKALQATLLSRNSVNDKYKALKSLIFGSQKPEDNFDANISEDGLI
	QLLAGKKVKVNKLFPQESNDASFTLNDKEDAIEEILGTLTPDECEWIAHIRRLFDWAIMK
	HALKDGRTISESKVKLYEQHHHDLTQLKYFVKTYLAKEYDDIFRNVDSETTKNYVAYSYH
	VKEVKGTLPKNKATQEEFCKYVLGKVKNIECSEADKVDFDEMIQRLTDNSFMPKQVSGEN
	RVIPYQLYYYELKTILNKAASYLPFLTQCGKDAISNQDKLLSIMTFRIPYFVGPLRKDNS
	EHAWLERKAGKIYPWNFNDKVDLDKSEEAFIRRMTNTCTYYPGEDVLPLDSLIYEKFMIL
	NEINNIRIDGYPISVDVKQQVFGLFEKKRRVTVKDIQNLLLSLGALDKHGKLTGIDTTIH
	SNYNTYHHFKSLMERGVLTRDDVERIVERMTYSDDTKRVRLWLNNNYGTLTADDVKHISR
	LRKHDFGRLSKMFLTGLKGVHKETGERASILDFMWNTNDNLMQLLSECYTFSDEITKLQE
	AYYAKAQLSLNDFLDSMYISNAVKRPIYRTLAVVNDIRKACGTAPKRIFIEMARDGESKK
	KRSVTRREQIKNLYRSIRKDFQQEVDFLEKILENKSDGQLQSDALYLYFAQLGRDMYTGD
	PIKLEHIKDQSFYNIDHIYPQSMVKDDSLDNKVLVQSEINGEKSSRYPLDAAIRNKMKPL
	WDAYYNHGLISLKKYQRLTRSTPFTDDEKWDFINRQLVETRQSTKALAILLKRKFPDTEI
	VYSKAGLSSDFRHEFGLVKSRNINDLHHAKDAFLAIVTGNVYHERFNRRWFMVNQPYSVK
	TKTLFTHSIKNGNFVAWNGEEDLGRIVKMLKQNKNTIHFTRFSFDRKEGLFDIQPLKAST
	GLVPRKAGLDVVKYGGYDKSTAAYYLLVRFTLEDKKTQHKLMMIPVEGLYKARIDHDKEF
	LTDYAQTTISEILQKDKQKVINIMFPMGTRHIKLNSMISIDGFYLSIGGKSSKGKSVLCH
	AMVPLIVPHKIECYIKAMESFARKFKENNKLRIVEKFDKITVEDNLNLYELFLQKLQHNP
	YNKFFSTQFDVLTNGRSTFTKLSPEEQVQTLLNILSIFKTCRSSGCDLKSINGSAQAARI
	MISADLTGLSKKYSDIRLVEQSASGLFVSKSQNLLEYL*

SEQ	MSSLTKFTNKYSKQLTIKNELIPVGKTLENIKENGLIDGDEQLNENYQKAKIIVDDFLRD
ID	FINKALNNTQIGNWRELADALNKEDEDNIEKLQDKIRGIIVSKFETFDLFSSYSIKKDEK
NO:	IIDDDNDVEEEELDLGKKTSSFKYIFKKNLFKLVLPSYLKTTNQDKLKIISSFDNFSTYF
2	RGFFENRKNIFTKKPISTSIAYRIVHDNFPKFLDNIRCFNVWQTECPQLIVKADNYLKSK
	NVIAKDKSLANYFTVGAYDYFLSQNGIDFYNNIIGGLPAFAGHEKIQGLNEFINQECQKD
	SELKSKLKNRHAFKMAVLFKQILSDREKSFVIDEFESDAQVIDAVKNFYAEQCKDNNVIF
	NLLNLIKNIAFLSDDELDGIFIEGKYLSSVSQKLYSDWSKLRNDIEDSANSKQGNKELAK
	KIKTNKGDVEKAISKYEFSLSELNSIVHDNTKFSDLLSCTLHKVASEKLVKVNEGDWPKH
	LKNNEEKQKIKEPLDALLEIYNTLLIFNCKSFNKNGNFYVDYDRCINELSSVVYLYNKTR
	NYCTKKPYNTDKFKLNFNSPQLGEGFSKSKENDCLTLLFKKDDNYYVGIIRKGAKINFDD
	TQAIADNTDNCIFKMNYFLLKDAKKFIPKCSIQLKEVKAHFKKSEDDYILSDKEKFASPL
	VIKKSTFLLATAHVKGKKGNIKKFQKEYSKENPTEYRNSLNEWIAFCKEFLKTYKAATIF
	DITTLKKAEEYADIVEFYKDVDNLCYKLEFCPIKTSFIENLIDNGDLYLFRINNKDFSSK
	STGTKNLHTLYLQAIFDERNLNNPTIMLNGGAELFYRKESIEQKNRITHKAGSILVNKVC
	KDGTSLDDKIRNEIYQYENKFIDTLSDEAKKVLPNVIKKEATHDITKDKRFTSDKFFFHC
	PLTINYKEGDTKQFNNEVLSFLRGNPDINIIGIDRGERNLIYVTVINQKGEILDSVSFNT
	VTNKSSKIEQTVDYEEKLAVREKERIEAKRSWDSISKIATLKEGYLSAIVHEICLLMIKH
	NAIVVLENLNAGFKRIRGGLSEKSVYQKFEKMLINKLNYFVSKKESDWNKPSGLLNGLQL
	SDQFESFEKLGIQSGFIFYVPAAYTSKIDPTTGFANVLNLSKVRNVDAIKSFFSNFNEIS
	YSKKEALFKFSFDLDSLSKKGFSSFVKFSKSKWNVYTFGERIIKPKNKQGYREDKRINLT
	FEMKKLLNEYKVSFDLENNLIPNLTSANLKDTFWKELFFIFKTTLQLRNSVTNGKEDVLI
	SPVKNAKGEFFVSGTHNKTLPQDCDANGAYHIALKGLMILERNNLVREEKDTKKIMAISN
	VDWFEYVQKRRGVL*

SEQ	MNNYDEFTKLYPIQKTIRFELKPQGRTMEHLETFNFFEEDRDRAEKYKILKEAIDEYHKK
ID	FIDEHLTNMSLDWNSLKQISEKYYKSREEKDKKVFLSEQKRMRQEIVSEFKKDDRFKDLF
NO:	SKKLFSELLKEEIYKKGNHQEIDALKSFDKFSGYFIGLHENRKNMYSDGDEITAISNRIV
3	NENFPKFLDNLQKYQEARKKYPEWIIKAESALVAHNIKMDEVFSLEYFNKVLNQEGIQRY
	NLALGGYVTKSGEKMMGLNDALNLAHQSEKSSKGRIHMTPLFKQILSEKESFSYIPDVFT
	EDSQLLPSIGGFFAQIENDKDGNIFDRALELISSYAEYDTERIYIRQADINRVSNVIFGE
	WGTLGGLMREYKADSINDINLERTCKKVDKWLDSKEFALSDVLEAIKRTGNNDAFNEYIS
	KMRTAREKIDAARKEMKFISEKISGDEESIHIIKTLLDSVQQFLHFFNLFKARQDIPLDG
	AFYAEFDEVHSKLFAIVPLYNKVRNYLTKNNLNTKKIKLNFKNPTLANGWDQNKVYDYAS
	LIFLRDGNYYLGIINPKRKKNIKFEQGSGNGPFYRKMVYKQIPGPNKNLPRVFLTSTKGK
	KEYKPSKEIIEGYEADKHIRGDKFDLDFCHKLIDFFKESIEKHKDWSKFNFYFSPTESYG
	DISEFYLDVEKQGYRMHFENISAETIDEYVEKGDLFLFQIYNKDFVKAATGKKDMHTIYW
	NAAFSPENLQDVVVKLNGEAELFYRDKSDIKEIVHREGEILVNRTYNGRTPVPDKIHKKL
	TDYHNGRTKDLGEAKEYLDKVRYFKAHYDITKDRRYLNDKIYFHVPLTLNFKANGKKNLN
	KMVIEKFLSDEKAHIIGIDRGERNLLYYSIIDRSGKIIDQQSLNVIDGFDYREKLNQREI
	EMKDARQSWNAIGKIKDLKEGYLSKAVHEITKMAIQYNAIVVMEELNYGFKRGRFKVEKQ
	IYQKFENMLIDKMNYLVFKDAPDESPGGVLNAYQLTNPLESFAKLGKQTGILFYVPAAYT
	SKIDPTTGFVNLFNTSSKTNAQERKEFLQKFESISYSAKDGGIFAFAFDYRKFGTSKTDH
	KNVWTAYTNGERMRYIKEKKRNELFDPSKEIKEALTSSGIKYDGGQNILPDILRSNNNGL
	IYTMYSSFIAAIQMRVYDGKEDYIISPIKNSKGEFFRTDPKRRELPIDADANGAYNIALR
	GELTMRAIAEKFDPDSEKMAKLELKHKDWFEFMQTRGD*

SEQ	MTKTFDSEFFNLYSLQKTVRFELKPVGETASFVEDFKNEGLKRVVSEDERRAVDYQKVKE
ID	IIDDYHRDFIEESLNYFPEQVSKDALEQAFHLYQKLKAAKVEEREKALKEWEALQKKLRE
NO:	KVVKCFSDSNKARFSRIDKKELIKEDLINWLVAQNREDDIPTVETFNNFTTYFTGFHENR
4	KNIYSKDDHATAISFRLIHENLPKFFDNVISFNKLKEGFPELKFDKVKEDLEVDYDLKHA
	FEIEYFVNFVTQAGIDQYNYLLGGKTLEDGTKKQGMNEQINLFKQQQTRDKARQIPKLIP
	LFKQILSERTESQSFIPKQFESDQELFDSLQKLHNNCQDKFTVLQQAILGLAEADLKKVF
	IKTSDLNALSNTIFGNYSVFSDALNLYKESLKTKKAQEAFEKLPAHSIHDLIQYLEQFNS
	SLDAEKQQSTDTVLNYFIKTDELYSRFIKSTSEAFTQVQPLFELEALSSKRRPPESEDEG
	AKGQEGFEQIKRIKAYLDTLMEAVHFAKPLYLVKGRKMIEGLDKDQSFYEAFEMAYQELE
	SLIIPIYNKARSYLSRKPFKADKFKINFDNNTLLSGWDANKETANASILFKKDGLYYLGI
	MPKGKTFLFDYFVSSEDSEKLKQRRQKTAEEALAQDGESYFEKIRYKLLPGASKMLPKVF
	FSNKNIGFYNPSDDILRIRNTASHTKNGTPQKGHSKVEFNLNDCHKMIDFFKSSIQKHPE
	WGSFGFTFSDTSDFEDMSAFYREVENQGYVISFDKIKETYIQSQVEQGNLYLFQIYNKDF
	SPYSKGKPNLHTLYWKALFEEANLNNVVAKLNGEAEIFFRRHSIKASDKVVHPANQAIDN
	KNPHTEKTQSTFEYDLVKDKRYTQDKFFFHVPISLNFKAQGVSKFNDKVNGFLKGNPDVN
	IIGIDRGERHLLYFTVVNQKGEILVQESLNTLMSDKGHVNDYQQKLDKKEQERDAARKSW
	TTVENIKELKEGYLSHVVHKLAHLIIKYNAIVCLEDLNFGFKRGRFKVEKQVYQKFEKAL
	IDKLNYLVFKEKELGEVGHYLTAYQLTAPFESFKKLGKQSGILFYVPADYTSKIDPTTGF
	VNFLDLRYQSVEKAKQLLSDFNAIRFNSVQNYFEFEIDYKKLTPKRKVGTQSKWVICTYG
	DVRYQNRRNQKGHWETEEVNVTEKLKALFASDSKTTTVIDYANDDNLIDVILEQDKASFF
	KELLWLLKLTMTLRHSKIKSEDDFILSPVKNEQGEFYDSRKAGEVWPKDADANGAYHIAL
	KGLWNLQQINQWEKGKTLNLAIKNQDWFSFIQEKPYQE*

SEQ	MHTGGLLSMDAKEFTGQYPLSKTLRFELRPIGRTWDNLEASGYLAEDRHRAECYPRAKEL
ID	LDDNHRAFLNRVLPQIDMDWHPIAEAFCKVHKNPGNKELAQDYNLQLSKRRKEISAYLQD
NO:	ADGYKGLFAKPALDEAMKIAKENGNESDIEVLEAFNGFSVYFTGYHESRENIYSDEDMVS
5	VAYRITEDNFPRFVSNALIFDKLNESHPDIISEVSGNLGVDDIGKYFDVSNYNNFLSQAG
	IDDYNHIIGGHTTEDGLIQAFNVVLNLRHQKDPGFEKIQFKQLYKQILSVRTSKSYIPKQ
	FDNSKEMVDCICDYVSKIEKSETVERALKLVRNISSFDLRGIFVNKKNLRILSNKLIGDW
	DAIETALMHSSSSENDKKSVYDSAEAFTLDDIFSSVKKFSDASAEDIGNRAEDICRVISE
	TAPFINDLRAVDLDSLNDDGYEAAVSKIRESLEPYMDLFHELEIFSVGDEFPKCAAFYSE
	LEEVSEQLIEIIPLFNKARSFCTRKRYSTDKIKVNLKFPTLADGWDLNKERDNKAAILRK
	DGKYYLAILDMKKDLSSIRTSDEDESSFEKMEYKLLPSPVKMLPKIFVKSKAAKEKYGLT
	DRMLECYDKGMHKSGSAFDLGFCHELIDYYKRCIAEYPGWDVFDFKFRETSDYGSMKEFN
	EDVAGAGYYMSLRKIPCSEVYRLLDEKSIYLFQIYNKDYSENAHGNKNMHTMYWEGLFSP
	QNLESPVFKLSGGAELFFRKSSIPNDAKTVHPKGSVLVPRNDVNGRRIPDSIYRELTRYF
	NRGDCRISDEAKSYLDKVKTKKADHDIVKDRRFTVDKMMFHVPIAMNFKAISKPNLNKKV
	IDGIIDDQDLKIIGIDRGERNLIYVTMVDRKGNILYQDSLNILNGYDYRKALDVREYDNK
	EARRNWTKVEGIRKMKEGYLSLAVSKLADMIIENNAIIVMEDLNHGFKAGRSKIEKQVYQ
	KFESMLINKLGYMVLKDKSIDQSGGALHGYQLANHVTTLASVGKQCGVIFYIPAAFTSKI
	DPTTGFADLFALSNVKNVASMREFFSKMKSVIYDKAEGKFAFTFDYLDYNVKSECGRTLW
	TVYTVGERFTYSRVNREYVRKVPTDIIYDALQKAGISVEGDLRDRIAESDGDTLKSIFYA
	FKYALDMRVENREEDYIQSPVKNASGEFFCSKNAGKSLPQDSDANGAYNIALKGILQLRM
	LSEQYDPNAESIRLPLITNKAWLTFMQSGMKTWKN*

SEQ	MDSLKDFTNLYPVSKTLRFELKPVGKTLENIEKAGILKEDEHRAESYRRVKKIIDTYHKV
ID	FIDSSLENMAKMGIENEIKAMLQSFCELYKKDHRTEGEDKALDKIRAVLRGLIVGAFTGV
NO:	CGRRENTVQNEKYESLFKEKLIKEILPDFVLSTEAESLPFSVEEATRSLKEFDSFTSYFA
6	GFYENRKNIYSTKPQSTAIAYRLIHENLPKFIDNILVFQKIKEPIAKELEHIRADFSAGG
	YIKKDERLEDIFSLNYYIHVLSQAGIEKYNALIGKIVTEGDGEMKGLNEHINLYNQQRGR
	EDRLPLFRPLYKQILSDREQLSYLPESFEKDEELLRALKEFYDHIAEDILGRTQQLMTSI
	SEYDLSRIYVRNDSQLTDISKKMLGDWNAIYMARERAYDHEQAPKRITAKYERDRIKALK
	GEESISLANLNSCIAFLDNVRDCRVDTYLSTLGQKEGPHGLSNLVENVFASYHEAEQLLS
	FPYPEENNLIQDKDNVVLIKNLLDNISDLQRFLKPLWGMGDEPDKDERFYGEYNYIRGAL
	DQVIPLYNKVRNYLTRKPYSTRKVKLNFGNSQLLSGWDRNKEKDNSCVILRKGQNFYLAI
	MNNRHKRSFENKVLPEYKEGEPYFEKMDYKFLPDPNKMLPKVFLSKKGIEIYKPSPKLLE
	QYGHGTHKKGDTFSMDDLHELIDFFKHSIEAHEDWKQFGFKFSDTATYENVSSFYREVED
	QGYKLSFRKVSESYVYSLIDQGKLYLFQIYNKDFSPCSKGTPNLHTLYWRMLFDERNLAD
	VIYKLDGKAEIFFREKSLKNDHPTHPAGKPIKKKSRQKKGEESLFEYDLVKDRHYTMDKF
	QFHVPITMNFKCSAGSKVNDMVNAHIREAKDMHVIGIDRGERNLLYICVIDSRGTILDQI
	SLNTINDIDYHDLLESRDKDRQQERRNWQTIEGIKELKQGYLSQAVHRIAELMVAYKAVV
	ALEDLNMGFKRGRQKVESSVYQQFEKQLIDKLNYLVDKKKRPEDIGGLLRAYQFTAPFKS
	FKEMGKQNGFLFYIPAWNTSNIDPTTGFVNLFHAQYENVDKAKSFFQKFDSISYNPKKDW
	FEFAFDYKNFTKKAEGSRSMWILCTHGSRIKNFRNSQKNGQWDSEEFALTEAFKSLFVRY
	EIDYTADLKTAIVDEKQKDFFVDLLKLFKLTVQMRNSWKEKDLDYLISPVAGADGRFFDT
	REGNKSLPKDADANGAYNIALKGLWALRQIRQTSEGGKLKLAISNKEWLQFVQERSYEKD
	*

SEQ	MNNGTNNFQNFIGISSLQKTLRNALIPTETTQQFIVKNGIIKEDELRGENRQILKDIMDD
ID	YYRGFISETLSSIDDIDWTSLFEKMEIQLKNGDNKDTLIKEQTEYRKAIHKKFANDDRFK
NO:	NMFSAKLISDILPEFVIHNNNYSASEKEEKTQVIKLFSRFATSFKDYFKNRANCFSADDI
7	SSSSCHRIVNDNAEIFFSNALVYRRIVKSLSNDDINKISGDMKDSLKEMSLEEIYSYEKY
	GEFITQEGISFYNDICGKVNSFMNLYCQKNKENKNLYKLQKLHKQILCIADTSYEVPYKF
	ESDEEVYQSVNGFLDNISSKHIVERLRKIGDNYNGYNLDKIYIVSKFYESVSQKTYRDWE
	TINTALEIHYNNILPGNGKSKADKVKKAVKNDLQKSITEINELVSNYKLCSDDNIKAETY
	IHEISHILNNFEAQELKYNPEIHLVESELKASELKNVLDVIMNAFHWCSVFMTEELVDKD
	NNFYAELEEIYDEIYPVISLYNLVRNYVTQKPYSTKKIKLNFGIPTLADGWSKSKEYSNN
	AIILMRDNLYYLGIFNAKNKPDKKIIEGNTSENKGDYKKMIYNLLPGPNKMIPKVFLSSK
	TGVETYKPSAYILEGYKQNKHIKSSKDFDITFCHDLIDYFKNCIAIHPEWKNFGFDFSDT
	STYEDISGFYREVELQGYKIDWTYISEKDIDLLQEKGQLYLFQIYNKDFSKKSTGNDNLH
	TMYLKNLFSEENLKDIVLKLNGEAEIFFRKSSIKNPIIHKKGSILVNRTYEAEEKDQFGN
	IQIVRKNIPENIYQELYKYFNDKSDKELSDEAAKLKNVVGHHEAATNIVKDYRYTYDKYF
	LHMPITINFKANKTGFINDRILQYIAKEKDLHVIGIDRGERNLIYVSVIDTCGNIVEQKS
	FNIVNGYDYQIKLKQQEGARQIARKEWKEIGKIKEIKEGYLSLVIHEISKMVIKYNAIIA
	MEDLSYGFKKGRFKVERQVYQKFETMLINKLNYLVFKDISITENGGLLKGYQLTYIPDKL
	KNVGHQCGCIFYVPAAYTSKIDPTTGFVNIFKFKDLTVDAKREFIKKFDSIRYDSEKNLF
	CFTFDYNNFITQNTVMSKSSWSVYTYGVRIKRRFVNGRFSNESDTIDITKDMEKTLEMTD
	INWRDGHDLRQDIIDYEIVQHIFEIFRLTVQMRNSLSELEDRDYDRLISPVLNENNIFYD
	SAKAGDALPKDADANGAYCIALKGLYEIKQITENWKEDGKFSRDKLKISNKDWFDFIQNK
	RYL*

SEQ	MTNKFTNQYSLSKTLRFELIPQGKTLEFIQEKGLLSQDKQRAESYQEMKKTIDKFHKYFI
ID	DLALSNAKLTHLETYLELYNKSAETKKEQKFKDDLKKVQDNLRKEIVKSFSDGDAKSIFA
NO:	ILDKKELITVELEKWFENNEQKDIYFDEKFKTFTTYFTGFHQNRKNMYSVEPNSTAIAYR
8	LIHENLPKFLENAKAFEKIKQVESLQVNFRELMGEFGDEGLIFVNELEEMFQINYYNDVL
	SQNGITIYNSIISGFTKNDIKYKGLNEYINNYNQTKDKKDRLPKLKQLYKQILSDRISLS
	FLPDAFTDGKQVLKAIFDFYKINLLSYTIEGQEESQNLLLLIRQTIENLSSFDTQKIYLK
	NDTHLTTISQQVFGDFSVFSTALNYWYETKVNPKFETEYSKANEKKREILDKAKAVFTKQ
	DYFSIAFLQEVLSEYILTLDHTSDIVKKHSSNCIADYFKNHFVAKKENETDKTFDFIANI
	TAKYQCIQGILENADQYEDELKQDQKLIDNLKFFLDAILELLHFIKPLHLKSESITEKDT
	AFYDVFENYYEALSLLTPLYNMVRNYVTQKPYSTEKIKLNFENAQLLNGWDANKEGDYLT
	TILKKDGNYFLAIMDKKHNKAFQKFPEGKENYEKMVYKLLPGVNKMLPKVFFSNKNIAYF
	NPSKELLENYKKETHKKGDTFNLEHCHTLIDFFKDSLNKHEDWKYFDFQFSETKSYQDLS
	GFYREVEHQGYKINFKNIDSEYIDGLVNEGKLFLFQIYSKDFSPFSKGKPNMHTLYWKAL
	FEEQNLQNVIYKLNGQAEIFFRKASIKPKNIILHKKKIKIAKKHFIDKKTKTSEIVPVQT
	IKNLNMYYQGKISEKELTQDDLRYIDNFSIFNEKNKTIDIIKDKRFTVDKFQFHVPITMN
	FKATGGSYINQTVLEYLQNNPEVKIIGLDRGERHLVYLTLIDQQGNILKQESLNTITDSK
	ISTPYHKLLDNKENERDLARKNWGTVENIKELKEGYISQVVHKIATLMLEENAIVVMEDL
	NFGFKRGRFKVEKQIYQKLEKMLIDKLNYLVLKDKQPQELGGLYNALQLTNKFESFQKMG
	KQSGFLFYVPAWNTSKIDPTTGFVNYFYTKYENVDKAKAFFEKFEAIRFNAEKKYFEFEV
	KKYSDFNPKAEGTQQAWTICTYGERIETKRQKDQNNKFVSTPINLTEKIEDFLGKNQIVY
	GDGNCIKSQIASKDDKAFFETLLYWFKMTLQMRNSETRTDIDYLISPVMNDNGTFYNSRD
	YEKLENPTLPKDADANGAYHIAKKGLMLLNKIDQADLTKKVDLSISNRDWLQFVQKNK*

SEQ	MEQEYYLGLDMGTGSVGWAVTDSEYHVLRKHGKALWGVRLFESASTAEERRMFRTSRRRL
ID	DRRNWRIEILQEIFAEEISKKDPGFFLRMKESKYYPEDKRDINGNCPELPYALFVDDDFT
NO:	DKDYHKKFPTIYHLRKMLMNTEETPDIRLVYLAIHHMMKHRGHFLLSGDINEIKEFGTTF
9	SKLLENIKNEELDWNLELGKEEYAVVESILKDNMLNRSTKKTRLIKALKAKSICEKAVLN
	LLAGGTVKLSDIFGLEELNETERPKISFADNGYDDYIGEVENELGEQFYIIETAKAVYDW
	AVLVEILGKYTSISEAKVATYEKHKSDLQFLKKIVRKYLTKEEYKDIFVSTSDKLKNYSA
	YIGMTKINGKKVDLQSKRCSKEEFYDFIKKNVLKKLEGQPEYEYLKEELERETFLPKQVN
	RDNGVIPYQIHLYELKKILGNLRDKIDLIKENEDKLVQLFEFRIPYYVGPLNKIDDGKEG
	KFTWAVRKSNEKIYPWNFENVVDIEASAEKFIRRMTNKCTYLMGEDVLPKDSLLYSKYMV
	LNELNNVKLDGEKLSVELKQRLYTDVFCKYRKVTVKKIKNYLKCEGIISGNVEITGIDGD
	FKASLTAYHDFKEILTGTELAKKDKENIITNIVLFGDDKKLLKKRLNRLYPQITPNQLKK
	ICALSYTGWGRFSKKFLEEITAPDPETGEVWNIITALWESNNNLMQLLSNEYRFMEEVET
	YNMGKQTKTLSYETVENMYVSPSVKRQIWQTLKIVKELEKVMKESPKRVFIEMAREKQES
	KRTESRKKQLIDLYKACKNEEKDWVKELGDQEEQKLRSDKLYLYYTQKGRCMYSGEVIEL
	KDLWDNTKYDIDHIYPQSKTMDDSLNNRVLVKKKYNATKSDKYPLNENIRHERKGFWKSL
	LDGGFISKEKYERLIRNTELSPEELAGFIERQIVETRQSTKAVAEILKQVFPESEIVYVK
	AGTVSRFRKDFELLKVREVNDLHHAKDAYLNIVVGNSYYVKFTKNASWFIKENPGRTYNL
	KKMFTSGWNIERNGEVAWEVGKKGTIVTVKQIMNKNNILVTRQVHEAKGGLFDQQIMKKG
	KGQIAIKETDERLASIEKYGGYNKAAGAYFMLVESKDKKGKTIRTIEFIPLYLKNKIESD
	ESIALNFLEKGRGLKEPKILLKKIKIDTLFDVDGFKMWLSGRTGDRLLFKCANQLILDEK
	IIVTMKKIVKFIQRRQENRELKLSDKDGIDNEVLMEIYNTFVDKLENTVYRIRLSEQAKT
	LIDKQKEFERLSLEDKSSTLFEILHIFQCQSSAANLKMIGGPGKAGILVMNNNISKCNKI
	SIINQSPTGIFENEIDLLK

SEQ	MNKFENFTGLYPISKTLRFELIPQGKTLEYIEKSEILENDNYRAEKYEEVKDIIDGYHKW
ID	FINETLHDLHINWSELKVALENNRIEKSDASKKELQRVQKIKREEIYNAFIEHEAFQYLF
NO:	KENLLSDLLPIQIEQSEDLDAEKKKQAVETFNRFSTYFTGFHENRKNIYSKEGISTSVTY
10	RIVHDNFPKFLENMKVFEILRNECPEVISDTANELAPFIDGVRIEDIFLIDFFNSTFSQN
	GIDYYNRILGGVTTETGEKYRGINEFTNLYRQQHPEFGKSKKATKMVVLFKQILSDRDTL
	SFIPEMFGNDKQVQNSIQLFYNREISQFENEGVKTDVCTALATLTSKIAEFDTEKIYIQQ
	PELPNVSQRLFGSWNELNACLFKYAELKFGTAEKVANRKKIDKWLKSDLFSFTELNKALE
	FSGKDERIENYFSETGIFAQLVKTGFDEAQSILETEYTSEVHLKDQQTDIEKIKTFLDAL
	QNLMHLLKSLCVSEEADRDAAFYNEFDMLYNQLKLVVPLYNKVRNYITQKLFRSDKIKIY
	FENKGQFLGGWVDSQTENSDNGTQAGGYIFRKENVINEYDYYLGICSDPKLFRRTTIVSE
	NDRSSFERLDYYQLKTASVYGNSYCGKHPYTEDKNELVNSIDRFVHLSGNNILIEKIAKD
	KVKSNPTTNTPSGYLNFIHREAPNTYECLLQDENFVSLNQRVVSALKATLATLVRVPKAL
	VYAKKDYHLFSEIINDIDELSYEKAFSYFPVSQTEFENSSNRTIKPLLLFKISNKDLSFA
	ENFEKGNRQKIGKKNLHTLYFEALMKGNQDTIDIGTGMVFHRVKSLNYNEKTLKYGHHST
	QLNEKFSYPIIKDKRFASDKFLFHLSTEINYKEKRKPLNNSIIEFLTNNPDINIIGLDRG
	ERHLIYLTLINQKGEILRQKTFNIVGNTNYHEKLNQREKERDNARKSWATIGKIKELKEG
	FLSLVIHEIAKIMVENNAIVVLEDLNFGFKRGRFKVEKQIYQKFEKMLIDKLNYLVFKDK
	KANEAGGVLKGYQLAEKFESFQKMGKQSGFLFYVPAAYTSKIDPTTGFVNMLNLNYTNMK
	DAQTLLSGMDKISFNADANYFEFELDYEKFKTNQTDHTNKWTICTVGEKRFTYNSATKET
	TTVNVTEDLKKLLDKFEVKYSNGDNIKDEICRQTDAKFFEIILWLLKLTMQMRNSNTKTE
	EDFILSPVKNSNGEFFRSNDDANGIWPADADANGAYHIALKGLYLVKECFNKNEKSLKIE
	HKNWFKFAQTRFNGSLTKNG*

SEQ	MENFKNLYPINKTLRFELRPYGKTLENFKKSGLLEKDAFKANSRRSMQAIIDEKFKETIE
ID	ERLKYTEFSECDLGNMTSKDKKITDKAATNLKKQVILSFDDEIFNNYLKPDKNIDALFKN
NO:	DPSNPVISTFKGFTTYFVNFFEIRKHIFKGESSGSMAYRIIDENLTTYLNNIEKIKKLPE
11	ELKSQLEGIDQIDKLNNYNEFITQSGITHYNEIIGGISKSENVKIQGINEGINLYCQKNK
	VKLPRLTPLYKMILSDRVSNSFVLDTIENDTELIEMISDLINKTEISQDVIMSDIQNIFI
	KYKQLGNLPGISYSSIVNAICSDYDNNFGDGKRKKSYENDRKKHLETNVYSINYISELLT
	DTDVSSNIKMRYKELEQNYQVCKENFNATNWMNIKNIKQSEKTNLIKDLLDILKSIQRFY
	DLFDIVDEDKNPSAEFYTWLSKNAEKLDFEFNSVYNKSRNYLTRKQYSDKKIKLNFDSPT
	LAKGWDANKEIDNSTIIMRKFNNDRGDYDYFLGIWNKSTPANEKIIPLEDNGLFEKMQYK
	LYPDPSKMLPKQFLSKIWKAKHPTTPEFDKKYKEGRHKKGPDFEKEFLHELIDCFKHGLV
	NHDEKYQDVFGFNLRNTEDYNSYTEFLEDVERCNYNLSFNKIADTSNLINDGKLYVFQIW
	SKDFSIDSKGTKNLNTIYFESLFSEENMIEKMFKLSGEAEIFYRPASLNYCEDIIKKGHH
	HAELKDKFDYPIIKDKRYSQDKFFFHVPMVINYKSEKLNSKSLNNRTNENLGQFTHIIGI
	DRGERHLIYLTVVDVSTGEIVEQKHLDEIINTDTKGVEHKTHYLNKLEEKSKTRDNERKS
	WEAIETIKELKEGYISHVINEIQKLQEKYNALIVMENLNYGFKNSRIKVEKQVYQKFETA
	LIKKFNYIIDKKDPETYIHGYQLTNPITTLDKIGNQSGIVLYIPAWNTSKIDPVTGFVNL
	LYADDLKYKNQEQAKSFIQKIDNIYFENGEFKFDIDFSKWNNRYSISKTKWTLTSYGTRI
	QTFRNPQKNNKWDSAEYDLTEEFKLILNIDGTLKSQDVETYKKFMSLFKLMLQLRNSVTG
	TDIDYMISPVTDKTGTHFDSRENIKNLPADADANGAYNIARKGIMAIENIMNGISDPLKI
	SNEDYLKYIQNQQE

SEQ	MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDHYKELKPIIDRIYKT
ID	YADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNAIHDYFIGRTDNLTDA
NO:	INKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDKFTTYFSGFYENRKNVF
12	SAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFENVKKAIGIFVSTSIEEV
	FSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLNLAIQKNDETAHIIASLPH
	RFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLRNENVLETAEALFNELNSID
	LTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGKITKSAKEKVQRSLKHEDINL
	QEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLKKQEEKEILKSQLDSLLGLYHL
	LDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKARNYATKKPYSVEKFKLNFQMPTL
	ASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKALSFEPTEKTSEGFDKMYYDYFPD
	AAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPLEITKEIYDLNNPEKEPKKFQTAYA
	KKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDLSSLRPSSQYKDLGEYYAELNPLLYH
	ISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGHHGKPNLHTLYWTGLFSPENLAKTSIK
	LNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKDQKTPIPDTLYQELYDYVNHRLSHDLSD
	EARALLPNVITKEVSHEIIKDRRFTSDKFFFHVPITLNYQAANSPSKFNQRVNAYLKEHP
	ETPIIGIDRGERNLIYITVIDSTGKILEQRSLNTIQQFDYQKKLDNREKERVAARQAWSV
	VGTIKDLKQGYLSQVIHEIVDLMIHYQAVVVLENLNFGFKSKRTGIAEKAVYQQFEKMLI
	DKLNCLVLKDYPAEKVGGVLNPYQLTDQFTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFV
	DPFVWKTIKNHESRKHFLEGFDFLHYDVKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVF
	EKNETQFDAKGTPFIAGKRIVPVIENHRFTGRYRDLYPANELIALLEEKGIVFRDGSNIL
	PKLLENDDSHAIDTMVALIRSVLQMRNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPM
	DADANGAYHIALKGQLLLNHLKESKDLKLQNGISNQDWLAYIQELRN*

SEQ	MAVKSIKVKLRLDDMPEIRAGLWKLHKEVNAGVRYYTEWLSLLRQENLYRRSPNGDGEQE
ID	CDKTAEECKAELLERLRARQVENGHRGPAGSDDELLQLARQLYELLVPQAIGAKGDAQQI
NO:	ARKFLSPLADKDAVGGLGIAKAGNKPRWVRMREAGEPGWEEEKEKAETRKSADRTADVLR
13	ALADFGLKPLMRVYTDSEMSSVEWKPLRKGQAVRTWDRDMFQQAIERMMSWESWNQRVGQ
	EYAKLVEQKNRFEQKNFVGQEHLVHLVNQLQQDMKEASPGLESKEQTAHYVTGRALRGSD
	KVFEKWGKLAPDAPFDLYDAEIKNVQRRNTRRFGSHDLFAKLAEPEYQALWREDASFLTR
	YAVYNSILRKLNHAKMFATFTLPDATAHPIWTRFDKLGGNLHQYTFLFNEFGERRHAIRF
	HKLLKVENGVAREVDDVTVPISMS
	EQLDNLLPRDPNEPIALYFRDYGAEQHFTGEFGGAKIQCRRDQLAHMHRRRGARDVYLNV
	SVRVQSQSEARGERRPPYAAVFRLVGDNHRAFVHFDKLSDYLAEHPDDGKLGSEGLLSGL
	RVMSVDLGLRTSASISVFRVARKDELKPNSKGRVPFFFPIKGNDNLVAVHERSQLLKLPG
	ETESKDLRAIREERQRTLRQLRTQLAYLRLLVRCGSEDVGRRERSWAKLIEQPVDAANHM
	TPDWREAFENELQKLKSLHGICSDKEWMDAVYESVRRVWRHMGKQVRDWRKDVRSGERPK
	IRGYAKDVVGGNSIEQIEYLERQYKFLKSWSFFGKVSGQVIRAEKGSRFAITLREHIDHA
	KEDRLKKLADRIIMEALGYVYALDERGKGKWVAKYPPCQLILLEELSEYQFNNDRPPSEN
	NQLMQWSHRGVFQELINQAQVHDLLVGTMYAAFSSRFDARTGAPGIRCRRVPARCTQEHN
	PEPFPWWLNKFVVEHTLDACPLRADDLIPTGEGEIFVSPFSAEEGDFHQIHADLNAAQNL
	QQRLWSDFDISQIRLRCDWGEVDGELVLIPRLTGKRTADSYSNKVFYTNTGVTYYERERG
	KKRRKVFAQEKLSEEEAELLVEADEAREKSVVLMRDPSGIINRGNWTRQKEFWSMVNQRI
	EGYLVKQIRSRVPLQDSACENTGDI*

SEQ	MATRSFILKIEPNEEVKKGLWKTHEVLNHGIAYYMNILKLIRQEAIYEHHEQDPKNPKKV
ID	SKAEIQAELWDFVLKMQKCNSFTHEVDKDVVFNILRELYEELVPSSVEKKGEANQLSNKF
NO:	LYPLVDPNSQSGKGTASSGRKPRWYNLKIAGDPSWEEEKKKWEEDKKKDPLAKILGKLAE
14	YGLIPLFIPFTDSNEPIVKEIKWMEKSRNQSVRRLDKDMFIQALERFLSWESWNLKVKEE
	YEKVEKEHKTLEERIKEDIQAFKSLEQYEKERQEQLLRDTLNTNEYRLSKRGLRGWREII
	QKWLKMDENEPSEKYLEVFKDYQRKHPREAGDYSVYEFLSKKENHFIWRNHPEYPYLYAT
	FCEIDKKKKDAKQQATFTLADPINHPLWVRFEERSGSNLNKYRILTEQLHTEKLKKKLTV
	QLDRLIYPTESGGWEEKGKVDIVLLPSRQFYNQIFLDIEEKGKHAFTYKDESIKFPLKGT
	LGGARVQFDRDHLRRYPHKVESGNVGRIYFNMTVNIEPTESPVSKSLKIHRDDFPKFVNF
	KPKELTEWIKDSKGKKLKSGIESLEIGLRVMSIDLGQRQAAAASIFEVVDQKPDIEGKLF
	FPIKGTELYAVHRASFNIKLPGETLVKSREVLRKAREDNLKLMNQKLNFLRNVLHFQQFE
	DITEREKRVTKWISRQENSDVPLVYQDELIQIRELMYKPYKDWVAFLKQLHKRLEVEIGK
	EVKHWRKSLSDGRKGLYGISLKNIDEIDRTRKFLLRWSLRPTEPGEVRRLEPGQRFAIDQ
	LNHLNALKEDRLKKMANTIIMHALGYCYDVRKKKWQAKNPACQIILFEDLSNYNPYEERS
	RFENSKLMKWSRREIPRQVALQGEIYGLQVGEVGAQFSSRFHAKTGSPGIRCSVVTKEKL
	QDNRFFKNLQREGRLTLDKIAVLKEGDLYPDKGGEKFISLSKDRKLVTTHADINAAQNLQ
	KRFWTRTHGFYKVYCKAYQVDGQTVYIPESKDQKQKIIEEFGEGYFILKDGVYEWGNAGK
	LKIKKGSSKQSSSELVDSDILKDSFDLASELKGEKLMLYRDPSGNVFPSDKWMAAGVFFG
	KLERILISKLTNQYSISTIEDDSSKQSM*

SEQ	MPTRTINLKLVLGKNPENATLRRALFSTHRLVNQATKRIEEFLLLCRGEAYRTVDNEGKE
ID	AEIPRHAVQEEALAFAKAAQRHNGCISTYEDQEILDVLRQLYERLVPSVNENNEAGDAQA
NO:	ANAWVSPLMSAESEGGLSVYDKVLDPPPVWMKLKEEKAPGWEAASQIWIQSDEGQSLLNK
15	PGSPPRWIRKLRSGQPWQDDFVSDQKKKQDELTKGNAPLIKQLKEMGLLPLVNPFFRHLL
	DPEGKGVSPWDRLAVRAAVAHFISWESWNHRTRAEYNSLKLRRDEFEAASDEFKDDFTLL
	RQYEAKRHSTLKSIALADDSNPYRIGVRSLRAWNRVREEWIDKGATEEQRVTILSKLQTQ
	LRGKFGDPDLFNWLAQDRHVHLWSPRDSVTPLVRINAVDKVLRRRKPYALMTFAHPRFHP
	RWILYEAPGGSNLRQYALDCTENALHITLPLLVDDAHGTWIEKKIRVPLAPSGQIQDLTL
	EKLEKKKNRLYYRSGFQQFAGLAGGAEVLFHRPYMEHDERSEESLLERPGAVWFKLTLDV
	ATQAPPNWLDGKGRVRTPPEVHHFKTALSNKSKHTRTLQPGLRVLSVDLGMRTFASCSVF
	ELIEGKPETGRAFPVADERSMDSPNKLWAKHERSFKLTLPGETPSRKEEEERSIARAEIY
	ALKRDIQRLKSLLRLGEEDNDNRRDALLEQFFKGWGEEDVVPGQAFPRSLFQGLGAAPFR
	STPELWRQHCQTYYDKAEACLAKHISDWRKRTRPRPTSREMWYKTRSYHGGKSIWMLEYL
	DAVRKLLLSWSLRGRTYGAINRQDTARFGSLASRLLHHINSLKEDRIKTGADSIVQAARG
	YIPL
	PHGKGWEQRYEPCQLILFEDLARYRFRVDRPRRENSQLMQWNHRAIVAETTMQAELYGQI
	VENTAAGFSSRFHAATGAPGVRCRFLLERDFDNDLPKPYLLRELSWMLGNTKVESEEEKL
	RLLSEKIRPGSLVPWDGGEQFATLHPKRQTLCVIHADMNAAQNLQRRFFGRCGEAFRLVC
	QPHGDDVLRLASTPGARLLGALQQLENGQGAFELVRDMGSTSQMNRFVMKSLGKKKIKPL
	QDNNGDDELEDVLSVLPEEDDTGRITVFRDSSGIFFPCNVWIPAKQFWPAVRAMIWKVMA
	SHSLG*

SEQ	MTKLRHRQKKLTHDWAGSKKREVLGSNGKLQNPLLMPVKKGQVTEFRKAFSAYARATKGE
ID	MTDGRKNMFTHSFEPFKTKPSLHQCELADKAYQSLHSYLPGSLAHFLLSAHALGFRIFSK
NO:	SGEATAFQASSKIEAYESKLASELACVDLSIQNLTISTLFNALTTSVRGKGEETSADPLI
16	ARFYTLLTGKPLSRDTQGPERDLAEVISRKIASSFGTWKEMTANPLQSLQFFEEELHALD
	ANVSLSPAFDVLIKMNDLQGDLKNRTIVFDPDAPVFEYNAEDPADIIIKLTARYAKEAVI
	KNQNVGNYVKNAITTTNANGLGWLLNKGLSLLPVSTDDELLEFIGVERSHPSCHALIELI
	AQLEAPELFEKNVFSDTRSEVQGMIDSAVSNHIARLSSSRNSLSMDSEELERLIKSFQIH
	TPHCSLFIGAQSLSQQLESLPEALQSGVNSADILLGSTQYMLTNSLVEESIATYQRTLNR
	INYLSGVAGQINGAIKRKAIDGEKIHLPAAWSELISLPFIGQPVIDVESDLAHLKNQYQT
	LSNEFDTLISALQKNFDLNFNKALLNRTQHFEAMCRSTKKNALSKPEIVSYRDLLARLTS
	CLYRGSLVLRRAGIEVLKKHKIFESNSELREHVHERKHFVFVSPLDRKAKKLLRLTDSRP
	DLLHVIDEILQHDNLENKDRESLWLVRSGYLLAGLPDQLSSSFINLPIITQKGDRRLIDL
	IQYDQINRDAFVMLVTSAFKSNLSGLQYRANKQSFVVTRTLSPYLGSKLVYVPKDKDWLV
	PSQMFEGRFADILQSDYMVWKDAGRLCVIDTAKHLSNIKKSVFSSEEVLAFLRELPHRTF
	IQTEVRGLGVNVDGIAFNNGDIPSLKTFSNCVQVKVSRTNTSLVQTLNRWFEGGKVSPPS
	IQFERAYYKKDDQIHEDAAKRKIRFQMPATELVHASDDAGWTPSYLLGIDPGEYGMGLSL
	VSINNGEVLDSGFIHINSLINFASKKSNHQTKVVPRQQYKSPYANYLEQSKDSAAGDIAH
	ILDRLIYKLNALPVFEALSGNSQSAADQVWTKVLSFYTWGDNDAQNSIRKQHWFGASHWD
	IKGMLRQPPTEKKPKPYIAFPGSQVSSYGNSQRCSCCGRNPIEQLREMAKDTSIKELKIR
	NSEIQLFDGTIKLFNPDPSTVIERRRHNLGPSRIPVADRTFKNISPSSLEFKELITIVSR
	SIRHSPEFIAKKRGIGSEYFCAYSDCNSSLNSEANAAANVAQKFQKQLFFEL*

SEQ	MKRILNSLKVAALRLLFRGKGSELVKTVKYPLVSPVQGAVEELAEAIRHDNLHLFGQKEI
ID	VDLMEKDEGTQVYSVVDFWLDTLRLGMFFSPSANALKITLGKFNSDQVSPFRKVLEQSPF
NO:	FLAGRLKVEPAERILSVEIRKIGKRENRVENYAADVETCFIGQLSSDEKQSIQKLANDIW
17	DSKDHEEQRMLKADFFAIPLIKDPKAVTEEDPENETAGKQKPLELCVCLVPELYTRGFGS
	IADFLVQRLTLLRDKMSTDTAEDCLEYVGIEEEKGNGMNSLLGTFLKNLQGDGFEQIFQF
	MLGSYVGWQGKEDVLRERLDLLAEKVKRLPKPKFAGEWSGHRMFLHGQLKSWSSNFFRLF
	NETRELLESIKSDIQHATMLISYVEEKGGYHPQLLSQYRKLMEQLPALRTKVLDPEIEMT
	HMSEAVRSYIMIHKSVAGFLPDLLESLDRDKDREFLLSIFPRIPKIDKKTKEIVAWELPG
	EPEEGYLFTANNLFRNFLENPKHVPRFMAERIPEDWTRLRSAPVWFDGMVKQWQKVVNQL
	VESPGALYQFNESFLRQRLQAMLTVYKRDLQTEKFLKLLADVCRPLVDFFGLGGNDIIFK
	SCQDPRKQWQTVIPLSVPADVYTACEGLAIRLRETLGFEWKNLKGHEREDFLRLHQLLGN
	LLFWIRDAKLVVKLEDWMNNPCVQEYVEARKAIDLPLEIFGFEVPIFLNGYLFSELRQLE
	LLLRRKSVMTSYSVKTTGSPNRLFQLVYLPLNPSDPEKKNSNNFQERLDTPTGLSRRFLD
	LTLDAFAGKLLTDPVTQELKTMAGFYDHLFGFKLPCKLAAMSNHPGSSSKMVVLAKPKKG
	VASNIGFEPIPDPAHPVFRVRSSWPELKYLEGLLYLPEDTPLTIELAETSVSCQSVSSVA
	FDLKNLTTILGRVGEFRVTADQPFKLTPIIPEKEESFIGKTYLGLDAGERSGVGFAIVTV
	DGDGYEVQRLGVHEDTQLMALQQVASKSLKEPVFQPLRKGTFRQQERIRKSLRGCYWNFY
	HALMIKYRAKVVHEESVGSSGLVGQWLRAFQKDLKKADVLPKKGGKNGVDKKKRESSAQD
	TLWGGAFSKKEEQQIAFEVQAAGSSQFCLKCGWWFQLGMREVNRVQESGVVLDWNRSIVT
	FLIESSGEKVYGFSPQQLEKGFRPDIETFKKMVRDFMRPPMFDRKGRPAAAYERFVLGRR
	HRRYRFDKVFEERFGRSALFICPRVGCGNFDHSSEQSAVVLALIGYIADKEGMSGKKLVY
	VRLAELMAEWKLKKLERSRVEEQSSAQ*

SEQ	MAESKQMQCRKCGASMKYEVIGLGKKSCRYMCPDCGNHTSARKIQNKKKRDKKYGSASKA
ID	QSQRIAVAGALYPDKKVQTIKTYKYPADLNGEVHDSGVAEKIAQAIQEDEIGLLGPSSEY
NO:	ACWIASQKQSEPYSVVDFWFDAVCAGGVFAYSGARLLSTVLQLSGEESVLRAALASSPFV
18	DDINLAQAEKFLAVSRRTGQDKLGKRIGECFAEGRLEALGIKDRMREFVQAIDVAQTAGQ
	RFAAKLKIFGISQMPEAKQWNNDSGLTVCILPDYYVPEENRADQLVVLLRRLREIAYCMG
	IEDEAGFEHLGIDPGALSNFSNGNPKRGFLGRLLNNDIIALANNMSAMTPYWEGRKGELI
	ERLAWLKHRAEGLYLKEPHFGNSWADHRSRIFSRIAGWLSGCAGKLKIAKDQISGVRTDL
	FLLKRLLDAVPQSAPSPDFIASISALDRFLEAAESSQDPAEQVRALYAFHLNAPAVRSIA
	NKAVQRSDSQEWLIKELDAVDHLEFNKAFPFFSDTGKKKKKGANSNGAPSEEEYTETESI
	QQPEDAEQEVNGQEGNGASKNQKKFQRIPRFFGEGSRSEYRILTEAPQYFDMFCNNMRAI
	FMQLESQPRKAPRDFKCFLQNRLQKLYKQTFLNARSNKCRALLESVLISWGEFYTYGANE
	KKFRLRHEASERSSDPDYVVQQALEIARRLFLFGFEWRDCSAGERVDLVEIHKKAISFLL
	AITQAEVSVGSYNWLGNSTVSRYLSVAGTDTLYGTQLEEFLNATVLSQMRGLAIRLSSQE
	LKDGFDVQLESSCQDNLQHLLVYRASRDLAACKRATCPAELDPKILVLPVGAFIASVMKM
	IERGDEPLAGAYLRHRPHSFGWQIRVRGVAEVGMDQGTALAFQKPTESEPFKIKPFSAQY
	GPVLWLNSSSYSQSQYLDGFLSQPKNWSMRVLPQAGSVRVEQRVALIWNLQAGKMRLERS
	GARAFFMPVPFSFRPSGSGDEAVLAPNRYLGLFPHSGGIEYAVVDVLDSAGFKILERGTI
	AVNGFSQKRGERQEEAHREKQRRGISDIGRKKPVQAEVDAANELHRKYTDVATRLGCRIV
	VQWAPQPKPGTAPTAQTVYARAVRTEAPRSGNQEDHARMKSSWGYTWGTYWEKRKPEDIL
	GISTQVYWTGGIGESCPAVAVALLGHIRATSTQTEWEKEEVVFGRLKKFFPS*

SEQ	MEKRINKIRKKLSADNATKPVSRSGPMKTLLVRVMTDDLKKRLEKRRKKPEVMPQVISNN
ID	AANNLRMLLDDYTKMKEAILQVYWQEFKDDHVGLMCKFAQPASKKIDQNKLKPEMDEKGN
NO:	LTTAGFACSQCGQPLFVYKLEQVSEKGKAYTNYFGRCNVAEHEKLILLAQLKPEKDSDEA
19	VTYSLGKFGQRALDFYSIHVTKESTHPVKPLAQIAGNRYASGPVGKALSDACMGTIASFL
	SKYQDIIIEHQKVVKGNQKRLESLRELAGKENLEYPSVTLPPQPHTKEGVDAYNEVIARV
	RMWVNLNLWQKLKLSRDDAKPLLRLKGFPSFPVVERRENEVDWWNTINEVKKLIDAKRDM
	GRVFWSGVTAEKRNTILEGYNYLPNENDHKKREGSLENPKKPAKRQFGDLLLYLEKKYAG
	DWGKVFDEAWERIDKKIAGLTSHIEREEARNAEDAQSKAVLTDWLRAKASFVLERLKEMD
	EKEFYACEIQLQKWYGDLRGNPFAVEAENRVVDISGFSIGSDGHSIQYRNLLAWKYLENG
	KREFYLLMNYGKKGRIRFTDGTDIKKSGKWQGLLYGGGKAKVIDLTFDPDDEQLIILPLA
	FGTRQGREFIWNDLLSLETGLIKLANGRVIEKTIYNKKIGRDEPALFVALTFERREVVDP
	SNIKPVNLIGVDRGENIPAVIALTDPEGCPLPEFKDSSGGPTDILRIGEGYKEKQRAIQA
	AKEVEQRRAGGYSRKFASKSRNLADDMVRNSARDLFYHAVTHDAVLVFENLSRGFGRQGK
	RTFMTERQYTKMEDWLTAKLAYEGLTSKTYLSKTLAQYTSKTCSNCGFTITTADYDGMLV
	RLKKTSDGWATTLNNKELKAEGQITYYNRYKRQTVEKELSAELDRLSEESGNNDISKWTK
	GRRDEALFLLKKRFSHRPVQEQFVCLDCGHEVHADEQAALNIARSWLFLNSNSTEFKSYK
	SGKQPFVGAWQAFYKRRLKEVWKPNA

SEQ	MKRINKIRRRLVKDSNTKKAGKTGPMKTLLVRVMTPDLRERLENLRKKPENIPQPISNTS
ID	RANLNKLLTDYTEMKKAILHVYWEEFQKDPVGLMSRVAQPAPKNIDQRKLIPVKDGNERL
NO:	TSSGFACSQCCQPLYVYKLEQVNDKGKPHTNYFGRCNVSEHERLILLSPHKPEANDELVT
20	YSLGKFGQRALDFYSIHVTRESNHPVKPLEQIGGNSCASGPVGKALSDACMGAVASFLTK
	YQDIILEHQKVIKKNEKRLANLKDIASANGLAFPKITLPPQPHTKEGIEAYNNVVAQIVI
	WVNLNLWQKLKIGRDEAKPLQRLKGFPSFPLVERQANEVDWWDMVCNVKKLINEKKEDGK
	VFWQNLAGYKRQEALLPYLSSEEDRKKGKKFARYQFGDLLLHLEKKHGEDWGKVYDEAWE
	RIDKKVEGLSKHIKLEEERRSEDAQSKAALTDWLRAKASFVIEGLKEADKDEFCRCELKL
	QKWYGDLRGKPFAIEAENSILDISGFSKQYNCAFIWQKDGVKKLNLYLIINYFKGGKLRF
	KKIKPEAFEANRFYTVINKKSGEIVPMEVNFNFDDPNLIILPLAFGKRQGREFIWNDLLS
	LETGSLKLANGRVIEKTLYNRRTRQDEPALFVALTFERREVLDSSNIKPMNLIGIDRGEN
	IPAVIALTDPEGCPLSRFKDSLGNPTHILRIGESYKEKQRTIQAAKEVEQRRAGGYSRKY
	ASKAKNLADDMVRNTARDLLYYAVTQDAMLIFENLSRGFGRQGKRTFMAERQYTRMEDWL
	TAKLAYEGLPSKTYLSKTLAQYTSKTCSNCGFTITSADYDRVLEKLKKTATGWMTTINGK
	ELKVEGQITYYNRYKRQNVVKDLSVELDRLSEESVNNDISSWTKGRSGEALSLLKKRFSH
	RPVQEKFVCLNCGFETHADEQAALNIARSWLFLRSQEYKKYQTNKTTGNTDKRAFVETWQ
	SFYRKKLKEVWKP

SEQ	atgGGAAAAATGTATTATCTTGGTCTGGATATAGGAACAAATTCTGTTGGATATGCCGTA
ID	ACCGACCCATCGTACCATTTGCTCAAATTTAAAGGCGAACCGATGTGGGGTGCCCACGTG
NO:	TTTGCTGCGGGGAATCAATCAGCTGAACGGAGAAGCTTTCGTACGAGCCGCAGACGCCTT
21	GACCGCAGGCAACAGCGTGTCAAACTGGTTCAAGAAATCTTTGCTCCCGTGATTAGTCCC
	ATTGATCCACGTTTTTTTATCAGACTTCATGAGAGCGCTTTATGGCGGGATGATGTGGCT
	GAAACGGATAAACATATTTTCTTTAATGACCCGACCTATACGGATAAGGAATATTATTCT
	GACTATCCAACCATCCATCATCTCATTGTGGACCTTATGGAAAGCAGTGAAAAGCATGAC
	CCGCGGCTTGTTTATTTGGCTGTTGCCTGGCTGGTTGCTCATCGTGGTCATTTCCTCAAT
	GAAGTGGATAAGGATAATATTGGGGATGTCCTGAGTTTTGACGCCTTTTATCCTGAGTTT
	CTGGCATTTCTTTCCGATAATGGGGTGTCACCTTGGGTATGTGAGTCAAAAGCACTCCAA
	GCGACCCTGCTTTCACGAAACTCCGTCAACGATAAGTATAAAGCCTTGAAGTCTCTGATC
	TTTGGCAGCCAAAAGCCGGAGGATAATTTTGATGCCAATATCAGTGAAGATGGACTTATC
	CAACTTTTAGCAGGAAAAAAGGTCAAGGTCAATAAACTTTTTCCTCAAGAAAGTAATGAT
	GCTTCCTTTACACTCAATGATAAGGAAGATGCAATTGAGGAAATCTTAGGAACGCTTACA
	CCGGATGAGTGTGAATGGATTGCGCATATTAGGAGGCTGTTTGATTGGGCCATCATGAAA
	CATGCTCTCAAAGATGGCAGAACAATCTCCGAATCGAAAGTAAAGCTCTATGAACAGCAT
	CACCATGACTTGACACAGCTCAAGTATTTTGTGAAGACCTATCTAGCAAAGGAATATGAT
	GACATTTTTCGAAACGTAGATAGTGAAACAACCAAAAACTATGTCGCATATTCCTATCAT
	GTAAAAGAAGTCAAGGGTACATTGCCCAAAAATAAGGCAACCCAAGAAGAATTTTGCAAG
	TATGTCCTTGGAAAGGTAAAGAACATCGAATGCAGTGAAGCTGATAAGGTTGATTTTGAT
	GAAATGATTCAGCGTCTTACAGACAATTCCTTTATGCCGAAACAAGTATCAGGTGAAAAC
	AGGGTTATCCCTTACCAGCTTTACTATTATGAACTAAAGACTATTTTGAATAAAGCCGCT
	TCTTATCTGCCTTTTTTGACCCAATGCGGAAAAGATGCCATCTCCAATCAAGATAAGCTC
	CTTTCCATCATGACCTTTCGGATTCCGTATTTCGTTGGGCCCTTGCGCAAGGACAATTCA
	GAGCATGCCTGGCTGGAACGAAAAGCAGGGAAAATCTATCCGTGGAATTTTAACGACAAA
	GTTGACCTTGATAAAAGTGAAGAAGCGTTCATTCGGAGAATGACGAATACCTGCACTTAT
	TATCCCGGTGAAGATGTTTTGCCACTTGACTCCCTTATTTATGAAAAATTCATGATCCTC
	AATGAAATCAATAATATCCGAATTGATGGTTATCCTATTTCTGTAGATGTAAAACAGCAG
	GTTTTTGGCCTCTTTGAAAAGAAGAGAAGAGTGACCGTAAAGGATATCCAGAATCTCCTG
	CTTTCCTTGGGTGCCTTGGATAAGCATGGTAAATTGACGGGAATCGATACTACCATCCAT
	AGCAATTACAATACATACCATCATTTTAAATCGCTCATGGAGCGTGGCGTTCTTACTCGT
	GATGATGTGGAACGCATTGTGGAGCGTATGACCTATAGTGATGATACAAAACGCGTCCGT
	CTTTGGCTGAACAATAATTATGGAACGCTCACTGCTGACGACGTAAAGCATATTTCAAGG
	CTCCGAAAGCATGATTTTGGCCGGCTTTCCAAAATGTTCCTCACAGGCCTAAAGGGAGTT
	CATAAGGAAACGGGGGAACGAGCTTCCATTTTGGATTTTATGTGGAATACCAATGATAAC
	TTGATGCAGCTTTTATCTGAATGTTATACTTTTTCGGATGAAATTACCAAGCTGCAGGAA
	GCATACTATGCCAAGGCGCAGCTTTCCCTGAATGATTTTCTGGACTCCATGTATATTTCA
	AATGCTGTCAAACGTCCTATCTATCGAACTCTTGCCGTTGTAAATGACATACGCAAAGCC
	TGTGGGACGGCGCCAAAACGCATTTTTATCGAAATGGCAAGAGATGGGGAAAGCAAAAAG
	AAAAGGAGCGTAACAAGAAGAGAACAAATCAAGAATCTTTATAGGTCCATCCGCAAGGAT
	TTTCAGCAGGAGGTAGATTTCCTTGAAAAAATCCTTGAAAACAAAAGCGATGGACAGCTG
	CAAAGCGATGCGCTCTATCTATACTTTGCGCAGCTTGGAAGGGATATGTATACCGGGGAC
	CCTATCAAGTTGGAGCATATCAAGGACCAGTCCTTCTATAATATTGATCATATCTATCCC
	CAAAGCATGGTCAAGGACGATAGTCTTGATAACAAGGTGTTGGTTCAATCGGAAATTAAT
	GGAGAGAAGAGCAGTCGATATCCTCTTGATGCTGCTATCCGTAATAAAATGAAGCCTCTT
	TGGGATGCTTATTATAACCATGGCCTGATTTCCCTCAAGAAGTATCAGCGTTTGACGCGG
	AGCACTCCCTTTACAGATGATGAAAAGTGGGATTTCATCAATCGGCAGCTTGTTGAGACA
	AGACAATCCACGAAGGCCTTGGCAATCTTACTAAAAAGGAAGTTCCCTGATACGGAGATT
	GTCTACTCCAAGGCAGGGCTTTCTTCTGATTTTCGGCATGAGTTTGGTCTCGTAAAATCG
	AGGAATATCAATGACCTGCACCATGCAAAGGACGCATTTCTTGCGATTGTAACAGGAAAT
	GTCTATCATGAACGCTTTAATCGCCGGTGGTTTATGGTGAACCAGCCCTATTCCGTCAAG
	ACCAAGACGTTGTTTACGCATTCTATTAAAAATGGTAATTTTGTAGCTTGGAATGGAGAA
	GAGGATCTTGGCCGCATTGTTAAAATGTTAAAGCAAAATAAGAACACTATTCATTTCACG
	CGGTTCTCTTTTGATCGAAAGGAAGGCCTGTTTGATATTCAGCCACTAAAAGCGTCAACC
	GGTCTTGTACCAAGAAAAGCCGGACTAGACGTGGTAAAATATGGTGGCTATGACAAATCG
	ACAGCAGCTTATTATCTCCTTGTTCGATTTACACTAGAAGATAAAAAGACTCAACATAAA
	TTGATGATGATTCCTGTAGAAGGCTTGTATAAAGCTCGAATTGACCATGATAAGGAATTC
	TTAACGGACTATGCACAAACTACAATCAGTGAAATCCTACAAAAAGATAAACAAAAGGTG
	ATAAATATAATGTTTCCAATGGGAACAAGGCACATTAAACTGAATTCCATGATTTCAATC
	GATGGTTTTTATCTTTCCATTGGAGGAAAGTCTAGTAAGGGAAAATCGGTGTTGTGTCAT
	GCTATGGTACCTCTTATTGTACCTCATAAGATAGAATGTTATATTAAGGCGATGGAGTCT
	TTTGCACGTAAATTTAAAGAAAATAATAAATTAAGGATTGTGGAAAAGTTTGATAAGATT
	ACGGTGGAAGATAACTTGAACCTATACGAACTATTTTTACAAAAACTTCAACATAACCCA
	TATAATAAGTTCTTCTCCACACAATTTGATGTGCTGACTAATGGAAGAAGTACATTTACT
	AAATTATCTCCAGAGGAACAAGTTCAAACGTTATTGAATATCTTATCAATTTTTAAAACT
	TGTCGGAGCTCTGGCTGCGATTTAAAATCCATTAACGGTTCTGCTCAAGCTGCCAGAATT
	ATGATCAGCGCAGATTTAACTGGACTCTCAAAAAAATATTCCGATATTCGGCTTGTTGAG
	CAATCAGCATCTGGACTTTTTGTTAGTAAATCACAAAATCTTTTGGAGTATTTAtga

SEQ	atgtcttcattaacaaaatttacaaataaatacagtaagcagctaaccataaaaaatgaa
ID	ctcatcccagtaggaaagactctcgagaacattaaggaaaacggtctcatagatggagat
NO:	gaacagctaaacgagaattatcaaaaagcaaagataatcgttgatgattttctacgagat
22	ttcataaataaagctttaaataatacccaaataggaaattggagagaattagcagatgct
	ttaaataaagaagatgaagataacatagaaaagctccaagacaaaatcagaggaataatt
	gtaagtaaattcgagacatttgatttgttttcttcttactcgataaagaaagacgaaaag
	ataatagatgatgataatgatgttgaagaagaggagctagatctaggaaaaaaaacttcc
	tcatttaaatatatttttaagaaaaacctttttaaattagtacttccttcttatttaaag
	acaacaaatcaggataaactgaaaataatctcttcttttgataatttttctacctatttc
	agaggattctttgagaacagaaaaaatattttcactaagaagcctatatctacgtcaatt
	gcctacagaattgtccatgataactttccaaagtttctagataacatcagatgttttaat
	gtgtggcaaacagaatgcccacagttaattgtaaaggctgataattatttaaaatcaaag
	aacgtcatagctaaagataaatctttagcaaactattttactgtaggagcatatgattac
	ttcttatcccagaatggcattgatttctacaacaacattatcggcggtctaccagcattt
	gctggtcatgagaaaatccaaggacttaatgaatttataaatcaagaatgccaaaaggac
	agcgaactaaaatctaaactgaaaaacagacatgctttcaaaatggctgttctatttaag
	caaattctttcagatagagaaaaaagttttgttatagacgagttcgaatctgatgctcag
	gtcatagatgcggttaagaacttctatgcagaacaatgtaaggataataatgttattttt
	aaccttctaaatcttatcaagaatatagcgttcttatctgatgatgaattagatggaatt
	tttatagaaggcaagtatttaagctctgtttcccaaaagctatattcagattggtcgaag
	cttcgaaatgatattgaagatagtgcaaacagtaaacaaggaaataaagagttagcaaag
	aaaattaaaacaaataaaggcgatgttgaaaaggccataagtaaatatgagttttcttta
	tcagaacttaactcaattgtacatgataatacaaaattcagtgaccttctttcttgtacg
	ttacataaagtggctagcgaaaaactagtgaaagttaatgaaggggactggccaaaacac
	ctgaaaaataatgaagaaaaacaaaagataaaagagcctttagatgcattgttagaaatt
	tataatacattgctgatattcaactgcaagtcatttaataagaacggtaatttctatgtt
	gattatgacagatgcataaatgagctttctagtgttgtttatttatataacaaaacaaga
	aattactgtacaaagaaaccttataacacagacaaattcaaattaaactttaacagtcct
	caattaggagagggctttagtaagtcgaaagaaaatgactgtctgacattattatttaaa
	aaagacgacaattactatgttggaattatcagaaaaggggcaaaaattaactttgatgat
	acacaagccattgcagacaatacagataactgtatatttaagatgaattatttcctatta
	aaagatgctaaaaagtttattcctaaatgttcaattcagttaaaagaagtaaaagcacat
	tttaaaaaatcagaggatgattatatcctgagtgacaaagaaaaatttgcctctcccctt
	gttattaagaaatcaacatttttattagcaacagcacatgtaaaaggaaagaaaggaaac
	ataaaaaaattccaaaaggaatattctaaggaaaatccaacagaatatagaaattctctg
	aatgaatggattgcattttgtaaagaatttctaaaaacatataaggcggcaacaatcttt
	gacattacaacgttaaaaaaagctgaagaatatgctgatattgttgagttttataaggat
	gtagataatctttgttataaactagagttttgccctattaaaacatctttcattgagaat
	cttattgataatggggacttatatttattcagaatcaataataaagatttcagttcaaaa
	tctactggtacaaagaatcttcatacgctctatcttcaggcaatctttgatgaaagaaac
	ctcaataatcctactattatgttaaatggcggagcagagttattttatcgaaaagaaagc
	attgaacagaaaaataggataactcataaggcaggatcaattcttgtaaacaaggtttgt
	aaggatggaacaagtctagatgacaaaatcagaaacgaaatatatcaatatgaaaacaag
	tttattgatacattgtctgatgaagctaaaaaagttttacctaatgtaataaaaaaagaa
	gcaactcacgacataacaaaagataagcgatttacatcagataagttctttttccattgc
	ccattaacaattaactataaggaaggagatacaaaacaatttaacaatgaggttttatct
	ttccttagaggtaatccagacattaatatcatcggaattgacagaggagaaagaaacctt
	atatacgtaactgttattaatcagaaaggcgaaatacttgacagcgtttcgtttaacaca
	gtaacaaacaagtcgagcaaaattgaacaaactgttgattatgaggaaaagcttgctgtt
	agggaaaaagaaagaatagaagcaaaaagatcctgggattcaatatcaaagatagcaacc
	ttaaaagaaggttatctatcagctattgttcatgagatatgcctactgatgatcaaacac
	aacgcaatcgttgtacttgagaatctaaatgcaggatttaagagaattagaggaggatta
	tcagaaaagtctgtttatcagaaattcgagaagatgcttattaacaaactaaattacttt
	gtatctaaaaaagaatcagactggaataaacctagtggacttttaaatggtttacaactt
	tcagaccagttcgagtcatttgagaaattaggaattcaatctgggttcatcttctatgtt
	cctgcagcatatacatctaagattgatcctacaacaggatttgcaaatgttcttaactta
	tccaaggtaagaaatgttgatgcaataaagagttttttcagtaatttcaatgaaatttca
	tatagcaaaaaagaagctctctttaaattctcttttgatttagattccttatcaaagaag
	ggcttcagctcatttgtaaaattcagtaaatctaaatggaatgtatatacatttggagag
	agaataataaaaccaaagaataagcaagggtatcgtgaagataagagaattaatttaaca
	tttgaaatgaaaaaacttctgaatgaatataaagtaagttttgatcttgaaaacaactta
	attccaaatctaacctctgcaaatctgaaagataccttctggaaagaactattctttatt
	tttaaaacaactctgcagcttagaaacagtgtaacaaatggcaaagaagatgtactgatt
	tctccagtaaagaacgctaaaggagagttctttgtatcaggaactcataacaagacatta
	cctcaagactgtgatgcaaatggagcatatcatatcgccctaaaaggtctgatgattctt
	gaacgtaacaatcttgttagagaagaaaaagacacaaagaagataatggcaatttctaat
	gttgactggtttgagtatgttcaaaaaaggagaggtgtcctgtaa

SEQ	ATGAACAACTATGATGAGTTTACCAAACTGTACCCAATACAGAAAACGATAAGGTTCGAA
ID	TTGAAGCCGCAGGGAAGAACGATGGAACACCTCGAAACATTCAACTTTTTCGAAGAGGAC
NO:	AGGGATAGAGCGGAGAAATATAAGATTTTAAAGGAAGCAATCGACGAGTATCATAAGAAG
23	TTTATAGACGAACATCTAACAAATATGTCTCTTGACTGGAATTCTTTAAAACAGATTTCA
	GAGAAATACTATAAGAGTAGAGAGGAAAAAGACAAGAAAGTTTTTCTGTCAGAACAGAAA
	CGCATGAGGCAAGAGATAGTTTCTGAGTTCAAAAAAGACGATCGGTTTAAAGATCTTTTT
	TCAAAAAAATTGTTTTCTGAACTTCTCAAGGAAGAGATTTACAAAAAAGGAAACCATCAG
	GAAATTGACGCATTGAAAAGTTTTGATAAATTCTCAGGCTATTTTATTGGGTTGCATGAG
	AACCGAAAAAATATGTATTCTGACGGAGACGAGATCACGGCTATCTCTAACCGTATTGTA
	AATGAGAATTTCCCGAAGTTCCTCGACAACCTTCAGAAATATCAGGAAGCTCGTAAAAAA
	TATCCAGAGTGGATCATTAAGGCAGAATCTGCTTTAGTTGCACATAATATCAAGATGGAT
	GAAGTCTTTTCCTTAGAGTATTTCAACAAAGTCCTGAATCAAGAAGGAATACAGAGATAC
	AATCTCGCCCTAGGTGGCTATGTGACCAAAAGTGGTGAGAAAATGATGGGGCTTAATGAT
	GCACTTAATCTTGCCCATCAAAGTGAAAAAAGCAGCAAGGGAAGGATACACATGACTCCA
	CTCTTCAAACAGATTCTGAGTGAAAAAGAGTCCTTTTCTTATATACCAGATGTTTTTACA
	GAAGACTCTCAACTTTTACCATCCATTGGTGGGTTCTTTGCACAAATAGAAAATGATAAG
	GACGGGAATATTTTTGACAGAGCATTAGAATTGATATCTTCTTATGCAGAATACGATACA
	GAAAGGATATATATCAGGCAAGCGGACATAAACAGAGTTTCTAATGTTATTTTCGGGGAG
	TGGGGAACACTGGGGGGGTTAATGAGGGAATACAAAGCAGACTCTATCAACGACATCAAT
	TTGGAGAGAACATGCAAGAAGGTAGACAAGTGGCTCGACTCAAAGGAGTTTGCGTTATCA
	GATGTATTAGAGGCAATAAAAAGAACCGGCAATAATGATGCTTTTAATGAATATATCTCA
	AAGATGCGCACTGCCAGGGAAAAGATTGACGCTGCAAGAAAGGAAATGAAATTCATTTCG
	GAAAAAATATCTGGAGACGAAGAATCGATCCATATTATCAAAACCTTATTGGACTCGGTG
	CAACAGTTTTTACATTTTTTCAATTTATTCAAAGCGCGTCAGGACATTCCTCTTGATGGA
	GCATTCTATGCGGAGTTCGATGAAGTCCATAGCAAACTGTTTGCTATTGTTCCGTTGTAT
	AATAAGGTTAGGAACTATCTTACGAAAAATAACCTTAACACGAAAAAGATAAAGCTAAAC
	TTCAAGAATCCAACTCTGGCAAACGGATGGGATCAAAACAAGGTATATGACTACGCCTCC
	TTAATCTTTCTCCGCGATGGTAATTATTATCTCGGAATAATAAATCCAAAAAGGAAAAAG
	AATATTAAATTCGAACAAGGGTCTGGAAATGGCCCATTCTACCGGAAGATGGTGTACAAA
	CAAATTCCAGGGCCGAACAAGAACTTACCAAGAGTCTTCCTCACATCTACGAAAGGCAAA
	AAAGAGTACAAGCCGTCAAAGGAGATAATAGAAGGATATGAAGCGGACAAACACATAAGA
	GGAGATAAATTCGATCTGGATTTCTGTCATAAGCTGATAGACTTCTTCAAGGAATCCATC
	GAGAAGCACAAGGACTGGAGTAAGTTCAACTTCTATTTCTCTCCAACTGAATCATATGGA
	GACATCAGCGAATTCTATCTGGATGTAGAAAAACAGGGATACCGGATGCATTTTGAGAAT
	ATTTCTGCCGAGACGATTGATGAGTATGTCGAAAAGGGGGACTTATTCCTCTTCCAGATA
	TACAACAAAGACTTTGTGAAAGCGGCAACCGGAAAAAAAGATATGCACACCATTTATTGG
	AACGCGGCATTCTCGCCCGAGAACCTTCAGGATGTGGTAGTGAAACTGAACGGTGAAGCA
	GAACTTTTCTACAGAGACAAGAGCGACATCAAGGAGATAGTTCACAGGGAGGGAGAGATA
	CTGGTCAATCGTACCTACAACGGCAGGACACCTGTGCCTGACAAGATCCACAAAAAATTA
	ACAGATTATCATAATGGCCGTACCAAAGATCTCGGAGAAGCAAAAGAATACCTCGATAAG
	GTCAGATATTTCAAAGCGCACTACGACATCACAAAGGATCGCAGATACCTGAATGATAAA
	ATATACTTCCATGTGCCTCTGACATTGAATTTCAAAGCAAACGGGAAGAAGAATCTCAAT
	AAGATGGTAATTGAAAAGTTCCTCTCGGACGAAAAAGCGCATATTATTGGGATTGATCGC
	GGGGAAAGGAATCTTCTTTACTATTCTATCATTGACAGGTCAGGTAAAATAATCGATCAA
	CAGAGCCTCAACGTCATCGATGGATTCGATTACCGAGAGAAACTGAATCAGAGGGAGATC
	GAGATGAAGGATGCCAGACAAAGCTGGAATGCTATCGGGAAGATAAAGGACCTCAAGGAA
	GGGTATCTTTCAAAAGCGGTCCACGAAATTACCAAGATGGCGATACAATACAATGCCATT
	GTTGTCATGGAGGAACTCAATTATGGGTTCAAACGCGGACGTTTCAAAGTTGAGAAGCAG
	ATATATCAGAAATTCGAGAATATGCTGATTGACAAGATGAATTATCTGGTATTCAAGGAT
	GCTCCGGATGAAAGTCCGGGAGGAGTCCTCAATGCATATCAGCTTACTAATCCGCTTGAA
	AGTTTCGCTAAACTTGGGAAACAGACAGGAATTCTTTTCTATGTTCCGGCAGCCTATACT
	TCGAAGATAGATCCGACGACCGGGTTTGTCAATCTTTTCAATACTTCAAGTAAAACGAAC
	GCACAGGAAAGAAAAGAATTCTTGCAAAAATTCGAGTCGATCTCCTATTCCGCTAAAGAC
	GGAGGAATATTCGCATTCGCGTTCGATTATCGGAAGTTCGGAACGTCAAAAACAGACCAC
	AAAAATGTATGGACCGCATACACGAACGGGGAAAGGATGAGGTACATAAAAGAGAAAAAA
	CGCAACGAACTGTTCGACCCCTCGAAGGAGATCAAAGAGGCTCTCACTTCATCAGGAATC
	AAATATGACGGCGGACAGAACATATTGCCAGATATCCTGAGGAGCAACAATAACGGTCTG
	ATCTACACAATGTATTCCTCTTTCATAGCGGCCATTCAAATGAGGGTCTATGACGGGAAA
	GAAGACTATATCATCTCGCCGATAAAGAACAGCAAGGGAGAGTTCTTCAGGACCGATCCG
	AAAAGAAGGGAACTTCCGATAGACGCGGATGCGAACGGCGCGTATAACATTGCTCTCAGG
	GGCGAATTGACGATGCGTGCGATAGCGGAGAAGTTCGATCCGGACTCGGAAAAGATGGCG
	AAGCTAGAACTGAAACATAAGGACTGGTTCGAATTCATGCAGACAAGGGGGGATTGA

SEQ	ATGACAAAAACATTTGATTCAGAATTTTTTAATTTATATTCTCTTCAAAAAACAGTTCGT
ID	TTTGAACTCAAGCCGGTTGGTGAAACAGCCTCGTTTGTTGAAGATTTTAAAAACGAAGGT
NO:	TTGAAACGAGTTGTTTCAGAGGATGAACGGCGTGCGGTTGATTACCAAAAAGTGAAAGAA
24	ATTATTGATGACTACCACCGAGATTTTATTGAAGAATCGCTGAACTATTTTCCTGAGCAG
	GTCTCAAAAGACGCTTTGGAACAAGCTTTTCACCTTTATCAAAAACTAAAAGCCGCTAAG
	GTTGAAGAGCGTGAAAAAGCATTGAAAGAATGGGAAGCCCTTCAGAAAAAACTGCGCGAA
	AAAGTTGTTAAATGTTTTTCAGATTCAAACAAAGCACGCTTTTCCCGCATTGATAAAAAA
	GAACTGATTAAAGAAGATTTAATTAACTGGTTGGTTGCACAAAATCGCGAAGATGACATT
	CCAACCGTTGAAACCTTTAACAACTTTACGACTTATTTTACGGGGTTTCATGAAAACCGA
	AAAAACATTTATTCAAAAGACGATCATGCCACAGCCATTTCATTTCGACTCATTCATGAA
	AACCTGCCTAAGTTTTTTGATAATGTGATCAGCTTTAATAAATTGAAGGAAGGATTTCCA
	GAGCTGAAATTTGATAAGGTTAAGGAAGATTTAGAAGTTGATTATGACTTGAAACATGCC
	TTTGAAATCGAATACTTTGTCAATTTTGTTACCCAAGCCGGAATTGACCAATATAACTAT
	CTTTTGGGGGGTAAAACCTTAGAAGACGGCACCAAAAAGCAAGGCATGAATGAACAAATC
	AATCTGTTCAAGCAACAGCAAACCCGAGACAAAGCCCGACAAATTCCCAAACTCATACCA
	TTGTTTAAACAAATTCTAAGCGAACGAACGGAAAGCCAATCGTTTATTCCAAAACAATTT
	GAATCAGACCAAGAGCTATTTGACTCACTGCAAAAACTGCATAACAACTGCCAAGATAAA
	TTTACCGTACTGCAACAAGCCATTTTAGGCTTAGCCGAAGCAGATCTGAAAAAAGTATTC
	ATTAAAACATCTGATCTTAATGCGCTATCAAATACCATTTTTGGAAATTACAGTGTGTTT
	TCGGATGCGTTGAATTTATACAAAGAATCGCTCAAAACAAAAAAGGCGCAAGAAGCGTTT
	GAAAAACTACCCGCTCACAGCATTCATGACTTGATTCAATATTTGGAGCAATTTAATAGC
	TCTTTGGATGCAGAAAAACAGCAATCAACTGACACCGTACTGAATTACTTTATTAAAACA
	GACGAGCTGTATTCTCGGTTCATAAAATCAACGAGCGAAGCCTTCACACAAGTACAACCA
	CTCTTTGAATTGGAAGCATTAAGCTCAAAACGTCGTCCACCGGAAAGTGAAGACGAAGGC
	GCAAAAGGTCAGGAAGGGTTTGAGCAAATTAAACGCATAAAAGCCTATTTGGATACCTTG
	ATGGAGGCGGTGCATTTTGCAAAACCACTTTATCTGGTGAAGGGGCGCAAAATGATTGAA
	GGTCTGGACAAAGACCAAAGTTTCTATGAAGCCTTTGAAATGGCTTACCAAGAACTAGAA
	AGTCTGATTATTCCAATCTACAACAAAGCTCGTAGTTATTTAAGTCGTAAACCGTTTAAA
	GCGGACAAATTCAAAATTAATTTTGATAATAATACATTGCTTTCCGGTTGGGATGCTAAT
	AAAGAAACGGCTAACGCTTCAATTTTGTTTAAGAAGGATGGTTTGTATTATTTAGGAATC
	ATGCCTAAAGGAAAAACGTTTTTGTTCGATTACTTCGTTTCATCGGAAGATTCTGAAAAG
	TTAAAACAAAGAAGACAAAAAACCGCCGAAGAAGCGCTTGCGCAAGATGGCGAAAGCTAC
	TTTGAAAAAATTCGTTACAAGCTGTTACCTGGCGCCAGCAAAATGTTGCCGAAAGTATTT
	TTTTCCAACAAAAACATAGGGTTTTACAACCCAAGTGATGACATACTTCGTATCAGGAAT
	ACAGCCTCTCACACTAAAAACGGAACACCGCAAAAAGGGCACTCTAAAGTAGAGTTTAAT
	TTGAATGATTGTCATAAGATGATTGATTTCTTTAAATCAAGCATTCAAAAGCATCCAGAG
	TGGGGAAGTTTTGGATTCACCTTTTCAGATACATCAGATTTTGAAGATATGAGCGCCTTT
	TATCGAGAAGTCGAAAACCAAGGTTATGTCATTAGTTTCGATAAAATAAAAGAAACTTAC
	ATTCAGAGTCAAGTTGAACAGGGGAACCTATATTTATTCCAAATCTACAATAAAGACTTC
	TCGCCCTACAGCAAAGGCAAACCAAATTTACACACGCTTTACTGGAAAGCGTTGTTTGAG
	GAAGCCAACCTAAATAATGTGGTGGCAAAACTCAATGGTGAAGCTGAAATTTTCTTTAGG
	CGACACTCAATCAAAGCATCTGATAAAGTGGTGCACCCAGCGAATCAAGCCATTGACAAT
	AAAAACCCGCATACCGAAAAAACGCAAAGCACCTTTGAATATGATCTTGTAAAAGACAAG
	CGCTATACCCAAGACAAATTCTTCTTCCATGTACCGATTTCATTGAACTTTAAGGCACAA
	GGTGTTTCAAAATTTAACGATAAAGTGAATGGATTTTTAAAGGGTAACCCAGATGTCAAT
	ATTATTGGCATTGACCGAGGCGAACGACACCTTCTGTATTTCACTGTGGTGAATCAGAAA
	GGTGAAATTTTGGTTCAAGAGTCGCTTAATACCCTAATGAGTGATAAAGGGCATGTGAAT
	GACTACCAGCAAAAACTCGACAAAAAAGAACAAGAACGCGATGCCGCTCGCAAAAGCTGG
	ACGACGGTTGAAAATATCAAAGAATTAAAAGAAGGCTATTTATCTCATGTTGTTCATAAG
	TTGGCACACCTGATTATTAAATACAATGCCATTGTTTGCTTGGAAGACCTGAATTTTGGT
	TTCAAACGCGGGCGTTTTAAAGTGGAAAAACAAGTTTATCAGAAATTTGAAAAAGCGCTT
	ATTGATAAGCTTAACTACTTGGTATTTAAAGAAAAAGAGTTAGGCGAGGTGGGCCATTAT
	CTAACCGCCTATCAGTTGACCGCACCGTTTGAAAGTTTCAAGAAGTTAGGCAAGCAAAGT
	GGCATATTGTTTTATGTTCCGGCGGATTACACCTCCAAAATTGACCCAACCACCGGGTTT
	GTCAACTTTCTTGATCTGCGTTATCAGAGTGTCGAAAAAGCCAAACAGCTCTTAAGCGAC
	TTTAATGCCATTCGTTTTAATTCAGTACAAAACTATTTTGAGTTCGAAATAGATTACAAA
	AAACTCACACCCAAACGTAAAGTTGGTACTCAGAGTAAATGGGTGATTTGTACCTATGGA
	GATGTCCGCTATCAAAATCGGCGTAATCAAAAAGGTCACTGGGAAACGGAAGAAGTCAAT
	GTGACTGAAAAACTAAAAGCCCTTTTCGCCAGTGATTCCAAAACTACAACCGTAATCGAT
	TACGCCAATGACGACAACCTAATTGACGTCATTCTGGAACAGGACAAAGCCAGCTTCTTC
	AAAGAACTGTTATGGTTATTAAAACTCACCATGACGCTCCGCCACAGCAAAATCAAAAGT
	GAAGACGACTTTATTCTTTCACCCGTTAAAAACGAACAAGGCGAGTTTTACGATAGTCGA
	AAAGCGGGCGAGGTGTGGCCTAAAGATGCAGACGCCAATGGCGCTTATCACATAGCGTTG
	AAAGGCTTGTGGAATCTGCAACAGATCAATCAGTGGGAAAAGGGTAAAACACTTAATCTG
	GCGATTAAAAACCAGGATTGGTTCAGTTTTATTCAAGAAAAGCCCTATCAAGAATAA

SEQ	ATGCACACAGGCGGATTACTTAGCATGGATGCCAAGGAGTTTACCGGACAGTACCCCCTT
ID	TCGAAGACTCTGCGTTTTGAACTGAGACCGATAGGCAGAACGTGGGACAATCTCGAAGCA
NO:	TCGGGGTATCTTGCGGAGGACAGACACCGTGCAGAATGCTATCCCAGGGCAAAAGAGCTC
25	TTGGACGACAACCATCGTGCATTCCTCAACCGTGTCCTGCCTCAGATCGATATGGATTGG
	CACCCGATCGCAGAGGCATTCTGCAAAGTCCACAAGAATCCGGGAAACAAGGAATTGGCT
	CAGGATTACAATCTTCAGCTGTCCAAACGCAGAAAGGAGATTTCGGCCTATCTGCAGGAT
	GCGGACGGCTATAAAGGTCTGTTTGCCAAACCTGCATTGGATGAAGCAATGAAGATCGCG
	AAAGAAAACGGAAATGAATCGGACATAGAGGTTCTTGAGGCATTCAACGGTTTCTCCGTA
	TACTTCACCGGATATCATGAGAGCAGGGAGAACATCTATTCGGACGAGGATATGGTGTCG
	GTAGCTTATCGCATCACCGAAGACAATTTCCCGAGATTCGTTTCCAATGCGCTTATATTC
	GATAAGCTGAATGAGTCGCACCCCGATATAATCTCGGAAGTATCCGGAAATCTGGGCGTA
	GACGACATCGGAAAATATTTTGATGTGTCTAACTACAATAATTTCCTGTCGCAGGCCGGT
	ATAGATGACTACAATCACATCATCGGCGGCCATACGACGGAGGACGGTCTGATCCAGGCA
	TTCAATGTTGTTCTGAATCTCAGGCATCAGAAAGACCCCGGATTCGAAAAAATCCAATTC
	AAACAGCTGTACAAACAGATACTCAGCGTCCGTACATCCAAATCCTATATCCCGAAACAG
	TTCGATAATTCGAAGGAGATGGTGGACTGCATCTGCGACTATGTGTCCAAGATCGAAAAA
	TCCGAAACGGTCGAGAGAGCATTGAAGCTGGTAAGGAACATATCTTCTTTTGATTTGCGC
	GGAATATTCGTAAACAAGAAGAATCTCCGCATTCTTTCCAACAAACTGATTGGTGATTGG
	GACGCGATCGAAACCGCGCTGATGCACTCCTCCTCTTCGGAAAATGATAAGAAATCCGTC
	TACGACAGCGCCGAGGCATTTACGCTGGATGATATCTTTTCGTCCGTTAAAAAATTCTCA
	GATGCATCTGCAGAGGATATCGGAAACCGGGCGGAGGACATATGCAGAGTCATATCTGAG
	ACCGCTCCGTTCATAAACGATCTGAGGGCTGTCGATTTGGACAGTTTGAATGACGACGGT
	TACGAGGCGGCGGTTTCCAAGATAAGGGAATCTCTGGAACCATATATGGATCTGTTTCAT
	GAACTGGAGATATTCTCCGTAGGCGATGAATTCCCGAAATGTGCAGCTTTCTACAGTGAA
	CTTGAAGAAGTCTCCGAACAGCTAATCGAGATTATACCGTTATTCAACAAGGCCCGTTCG
	TTCTGTACGCGCAAGAGATACAGTACGGACAAGATAAAGGTCAATTTGAAATTCCCGACA
	CTCGCCGACGGATGGGATCTCAACAAAGAACGCGACAACAAAGCCGCAATACTCAGGAAA
	GACGGAAAGTACTACCTGGCCATACTGGATATGAAGAAAGATCTTTCTTCGATCAGAACT
	TCGGATGAAGACGAATCCAGTTTTGAGAAAATGGAGTACAAGCTTCTTCCGAGTCCGGTA
	AAGATGCTGCCAAAGATCTTCGTAAAATCGAAGGCGGCCAAGGAGAAGTACGGTCTGACC
	GACCGTATGCTGGAGTGCTACGATAAAGGGATGCACAAGAGCGGCAGTGCATTCGATCTC
	GGATTTTGTCACGAATTGATCGATTACTACAAGAGGTGCATCGCAGAATATCCCGGCTGG
	GACGTCTTCGATTTCAAGTTCAGGGAAACATCGGATTATGGCAGCATGAAGGAGTTCAAT
	GAGGATGTTGCAGGGGCCGGATACTATATGTCCCTCAGAAAGATCCCTTGTTCGGAGGTC
	TACAGGCTTCTTGATGAGAAATCGATATATCTTTTCCAGATCTACAACAAAGATTATTCG
	GAAAACGCTCATGGGAATAAGAACATGCATACCATGTATTGGGAAGGGCTCTTTTCCCCC
	CAGAATCTGGAATCCCCTGTGTTTAAACTCAGCGGCGGTGCGGAGCTTTTCTTCCGTAAA
	TCCTCCATACCCAATGACGCCAAAACGGTCCATCCGAAGGGAAGCGTCCTGGTTCCGCGC
	AATGATGTAAACGGCCGCAGGATACCTGACAGCATATATCGGGAGCTCACCAGATATTTC
	AACCGCGGAGATTGCCGCATAAGCGACGAGGCAAAGAGTTATCTGGACAAGGTGAAAACC
	AAGAAAGCTGACCACGATATCGTGAAAGACAGGAGGTTCACGGTGGACAAGATGATGTTC
	CACGTCCCTATCGCCATGAATTTCAAAGCGATTTCGAAGCCGAATCTCAATAAAAAGGTG
	ATTGACGGCATAATCGACGACCAAGATCTGAAGATCATCGGCATAGACCGCGGAGAGCGC
	AACCTCATCTACGTAACCATGGTGGATCGCAAAGGGAACATCCTCTATCAGGATAGCCTC
	AATATTCTGAACGGATACGATTACCGTAAGGCCCTCGACGTCCGCGAATATGACAATAAA
	GAGGCTCGGAGGAACTGGACGAAGGTCGAAGGCATCCGTAAGATGAAAGAGGGGTATCTG
	TCGCTTGCAGTCAGCAAATTGGCAGATATGATCATAGAGAACAATGCGATTATCGTCATG
	GAGGATCTCAATCACGGATTCAAGGCAGGGCGTTCGAAGATAGAGAAACAGGTCTATCAG
	AAGTTCGAATCCATGCTCATAAACAAACTCGGTTACATGGTCCTCAAGGATAAGTCTATC
	GATCAGAGCGGCGGAGCTCTCCACGGATACCAGCTTGCCAACCATGTGACAACATTGGCA
	TCTGTAGGTAAACAATGTGGAGTGATATTCTACATCCCTGCTGCATTTACATCCAAGATA
	GATCCGACAACAGGATTTGCAGATCTGTTCGCCCTCAGCAATGTTAAAAACGTGGCATCT
	ATGAGAGAATTTTTCTCCAAGATGAAGTCTGTAATCTATGATAAGGCGGAGGGAAAATTC
	GCATTTACCTTCGACTATCTTGATTATAATGTGAAATCCGAGTGCGGAAGGACCCTTTGG
	ACCGTGTATACGGTCGGAGAGAGATTCACATACAGCAGGGTCAATAGAGAATATGTCAGA
	AAAGTTCCGACAGACATAATCTACGACGCATTGCAAAAGGCAGGAATATCTGTTGAAGGG
	GATCTCAGGGACAGGATTGCTGAATCGGATGGCGACACTCTGAAGAGCATATTCTATGCA
	TTCAAGTATGCATTGGATATGAGAGTAGAGAACCGCGAAGAGGATTACATACAGTCTCCT
	GTCAAAAATGCCTCCGGAGAATTCTTCTGTTCCAAGAACGCAGGCAAATCGCTCCCTCAG
	GATTCCGATGCGAACGGTGCATACAATATCGCACTCAAGGGGATCCTGCAGCTACGTATG
	CTTTCCGAGCAGTATGATCCGAATGCAGAGAGCATACGGTTGCCACTGATAACCAACAAG
	GCCTGGCTGACCTTTATGCAGTCCGGTATGAAGACATGGAAGAACTGA

SEQ	atgGATAGTTTGAAAGATTTCACCAATCTGTACCCTGTCAGTAAGACATTGAGATTTGAA
ID	TTAAAGCCCGTTGGAAAGACTTTAGAAAATATCGAGAAAGCAGGTATTTTGAAAGAGGAT
NO:	GAGCATCGTGCAGAAAGTTATCGGAGGGTGAAGAAAATAATTGATACTTATCATAAGGTA
26	TTTATCGATTCTTCTCTTGAAAATATGGCTAAAATGGGTATTGAGAATGAAATAAAAGCA
	ATGCTCCAAAGTTTCTGCGAATTGTATAAAAAAGATCATCGCACTGAGGGTGAAGACAAG
	GCATTAGATAAAATTCGAGCAGTACTTCGTGGCCTGATTGTTGGGGCTTTCACTGGTGTT
	TGCGGAAGACGGGAAAATACAGTCCAAAACGAGAAGTACGAGAGTTTGTTCAAAGAAAAG
	TTGATAAAAGAAATTTTACCTGATTTTGTGCTCTCTACTGAGGCTGAAAGCTTGCCTTTC
	TCTGTTGAAGAAGCTACGAGGTCACTGAAGGAGTTTGATAGCTTTACATCCTACTTTGCT
	GGTTTTTACGAGAATAGAAAGAATATATACTCGACGAAACCTCAATCCACTGCCATTGCT
	TATCGTCTTATTCATGAGAACTTGCCGAAGTTCATTGATAATATTCTTGTTTTTCAGAAG
	ATCAAAGAGCCTATAGCCAAAGAGCTGGAACATATTCGTGCGGACTTTTCTGCCGGGGGG
	TACATAAAAAAGGATGAGAGATTGGAGGATATTTTTTCGTTGAACTATTATATCCACGTG
	TTATCTCAGGCTGGGATCGAAAAATATAACGCATTGATTGGGAAGATTGTGACAGAAGGA
	GATGGAGAGATGAAAGGGCTCAATGAACACATCAACCTTTACAACCAACAAAGAGGCAGA
	GAGGATCGGCTCCCTCTTTTTAGGCCTCTTTATAAACAGATATTGAGTGACAGAGAGCAA
	TTATCATACTTGCCTGAGAGTTTTGAAAAAGATGAGGAGCTCCTCAGGGCTCTAAAAGAG
	TTCTATGATCATATCGCAGAAGACATTCTCGGACGTACTCAACAGTTGATGACTTCTATT
	TCAGAATATGATTTATCTCGGATATACGTAAGGAACGATAGCCAATTGACTGATATATCA
	AAAAAAATGTTGGGAGATTGGAATGCTATCTACATGGCTAGAGAACGAGCATATGACCAC
	GAGCAGGCTCCCAAAAGAATCACGGCGAAATACGAGAGGGACAGGATTAAAGCTCTTAAA
	GGAGAAGAGAGTATAAGTCTGGCAAATCTTAATAGTTGTATTGCCTTTCTGGACAATGTT
	AGAGATTGCCGTGTAGATACTTATCTTTCCACACTGGGCCAGAAGGAAGGACCACATGGT
	CTATCTAATCTCGTTGAGAACGTTTTTGCCTCATACCATGAAGCAGAGCAATTGTTGAGC
	TTTCCATACCCCGAAGAGAATAATCTGATTCAGGACAAGGACAATGTGGTGTTAATTAAG
	AATCTTCTCGACAATATCAGTGATCTGCAGAGGTTCTTGAAACCTCTTTGGGGTATGGGA
	GACGAACCCGATAAAGATGAAAGATTTTATGGAGAGTATAATTATATCCGAGGAGCTCTA
	GATCAGGTGATCCCTCTGTACAATAAGGTAAGGAACTACCTCACTCGGAAGCCTTATTCG
	ACCAGAAAAGTAAAACTCAATTTTGGGAATTCTCAATTGCTTAGTGGTTGGGATAGAAAT
	AAGGAAAAGGATAATAGCTGTGTGATTTTGCGTAAGGGGCAGAACTTCTATTTGGCTATT
	ATGAACAATAGGCACAAAAGAAGTTTCGAAAACAAGGTGTTGCCCGAGTATAAGGAGGGA
	GAACCTTACTTCGAAAAGATGGATTATAAATTTTTGCCTGATCCTAATAAAATGCTTCCT
	AAGGTTTTTCTTTCGAAAAAAGGAATAGAGATATACAAACCAAGTCCGAAGCTTTTAGAA
	CAATATGGACATGGAACTCACAAAAAGGGAGATACCTTTAGTATGGATGATTTGCACGAA
	CTGATCGATTTCTTCAAACACTCAATCGAGGCTCATGAAGATTGGAAGCAATTCGGATTC
	AAATTTTCTGATACGGCTACTTATGAGAATGTATCTAGTTTCTATAGAGAAGTTGAGGAT
	CAGGGGTATAAGCTCTCTTTCCGAAAAGTTTCGGAATCTTATGTCTATTCATTAATAGAT
	CAAGGCAAGTTGTATTTATTTCAGATATACAACAAGGACTTTTCTCCCTGCAGCAAAGGG
	ACACCTAATCTGCATACCTTGTATTGGAGAATGCTTTTTGACGAGCGCAATTTGGCAGAT
	GTCATATACAAACTGGATGGGAAGGCTGAAATCTTTTTCCGAGAGAAGAGTTTGAAAAAT
	GATCATCCCACGCATCCGGCTGGTAAGCCTATCAAAAAGAAAAGTCGACAAAAAAAAGGA
	GAGGAGAGTCTGTTTGAGTATGATTTAGTCAAGGATAGGCACTATACGATGGATAAGTTC
	CAGTTTCATGTGCCTATTACTATGAATTTTAAATGTTCTGCAGGAAGCAAAGTCAATGAT
	ATGGTTAATGCTCATATTCGAGAGGCAAAGGATATGCATGTCATTGGAATTGATCGTGGA
	GAACGCAATCTGCTGTATATATGCGTGATAGATAGTCGAGGGACGATTTTGGATCAAATT
	TCTCTGAATACGATTAACGATATAGACTATCATGATTTATTGGAGAGTCGAGACAAAGAC
	CGTCAGCAGGAGCGCCGAAACTGGCAAACTATCGAAGGGATCAAGGAGCTAAAACAAGGC
	TACCTTAGTCAGGCGGTTCATCGGATAGCCGAACTGATGGTGGCTTATAAGGCTGTAGTT
	GCTTTGGAGGATTTGAATATGGGGTTCAAACGTGGGCGGCAGAAAGTAGAAAGTTCTGTT
	TATCAGCAGTTTGAGAAACAGCTGATAGATAAGCTCAACTATCTTGTGGACAAGAAGAAA
	AGGCCTGAAGATATTGGAGGATTGTTGAGAGCCTATCAATTTACGGCCCCATTTAAGAGT
	TTTAAGGAAATGGGAAAGCAAAACGGCTTCTTGTTTTATATCCCGGCTTGGAACACGAGC
	AACATAGATCCGACTACTGGATTTGTTAATTTATTTCATGCCCAGTATGAAAATGTAGAT
	AAAGCGAAGAGCTTCTTTCAAAAGTTTGATTCAATTAGTTACAACCCGAAGAAAGACTGG
	TTTGAGTTTGCATTCGATTATAAAAACTTTACTAAAAAGGCTGAAGGAAGTCGTTCTATG
	TGGATATTATGCACACATGGTTCCCGAATAAAGAATTTTAGAAATTCCCAGAAGAATGGT
	CAATGGGATTCCGAAGAATTCGCCTTGACGGAGGCTTTTAAGTCTCTTTTTGTGCGATAT
	GAGATAGATTATACCGCTGATTTGAAAACAGCTATTGTGGACGAAAAGCAAAAAGACTTC
	TTCGTGGATCTTCTGAAGCTATTCAAATTGACAGTACAGATGCGCAACAGCTGGAAAGAG
	AAGGATTTGGATTATCTAATCTCTCCTGTAGCAGGGGCTGATGGCCGTTTCTTCGATACA
	AGAGAGGGAAATAAAAGTCTGCCTAAGGATGCAGATGCCAATGGAGCTTATAATATTGCC
	CTAAAAGGACTTTGGGCTCTACGCCAGATTCGGCAAACTTCAGAAGGCGGTAAACTCAAA
	TTGGCGATTTCCAATAAGGAATGGCTACAGTTTGTGCAAGAGAGATCTTACGAGAAAGAC
	tga

SEQ	atgaataatggaacaaataactttcagaattttatcggaatttcttctttgcagaagact
ID	cttaggaatgctctcattccaaccgaaacaacacagcaatttattgttaaaaacggaata
NO:	attaaagaagatgagctaagaggagaaaatcgtcagatacttaaagatatcatggatgat
27	tattacagaggtttcatttcagaaactttatcgtcaattgatgatattgactggacttct
	ttatttgagaaaatggaaattcagttaaaaaatggagataacaaagacactcttataaaa
	gaacagactgaataccgtaaggcaattcataaaaaatttgcaaatgatgatagatttaaa
	aatatgttcagtgcaaaattaatctcagatattcttcctgaatttgtcattcataacaat
	aattattctgcatcagaaaaggaagaaaaaacacaggtaattaaattattttccagattt
	gcaacgtcattcaaggactattttaaaaacagggctaattgtttttcggctgatgatata
	tcttcatcttcttgtcatagaatagttaatgataatgcagagatattttttagtaatgca
	ttggtgtataggagaattgtaaaaagtctttcaaatgatgatataaataaaatatccgga
	gatatgaaggattcattaaaggaaatgtctctggaagaaatttattcttatgaaaaatat
	ggggaatttattacacaggaaggtatatctttttataatgatatatgtggtaaagtaaat
	tcatttatgaatttatattgccagaaaaataaagaaaacaaaaatctctataagctgcaa
	aagcttcataaacagatactgtgcatagcagatacttcttatgaggtgccgtataaattt
	gaatcagatgaagaggtttatcaatcagtgaatggatttttggacaatattagttcgaaa
	catatcgttgaaagattgcgtaagattggagacaactataacggctacaatcttgataag
	atttatattgttagtaaattctatgaatcagtttcacaaaagacatatagagattgggaa
	acaataaatactgcattagaaattcattacaacaatatattacccggaaatggtaaatct
	aaagctgacaaggtaaaaaaagcggtaaagaatgatctgcaaaaaagcattactgaaatc
	aatgagcttgttagcaattataaattatgttcggatgataatattaaagctgagacatat
	atacatgaaatatcacatattttgaataattttgaagcacaggagcttaagtataatcct
	gaaattcatctggtggaaagtgaattgaaagcatctgaattaaaaaatgttctcgatgta
	ataatgaatgcttttcattggtgttcggttttcatgacagaggagctggtagataaagat
	aataatttttatgccgagttagaagagatatatgacgaaatatatccggtaatttcattg
	tataatcttgtgcgtaattatgtaacgcagaagccatatagtacaaaaaaaattaaattg
	aattttggtattcctacactagcggatggatggagtaaaagtaaagaatatagtaataat
	gcaattattctcatgcgtgataatttgtactatttaggaatatttaatgcaaaaaataag
	cctgacaaaaagataattgaaggtaatacatcagaaaataaaggggattataagaagatg
	atttataatcttctgccaggaccaaataaaatgatccccaaggtattcctctcttcaaaa
	accggagtggaaacatataagccgtctgcctatatattggagggctataaacaaaacaag
	catattaaatcctctaaggattttgatataacattttgtcacgatttgattgattatttt
	aagaactgtatagcaatacatcctgaatggaagaattttggctttgatttttctgacacc
	tccacatatgaagatatcagcggattttacagagaagtcgaattacaaggttataaaatc
	gactggacatatatcagcgaaaaggatattgatttgttgcaggaaaaaggacagttatat
	ttattccaaatatataacaaagatttttccaagaaaagtaccggaaatgataatcttcat
	actatgtatttgaagaatttgtttagtgaagagaatttaaaggatattgtactgaaatta
	aacggtgaggcggaaatcttctttagaaaatcaagcataaagaatccaataattcataaa
	aaaggctctattcttgttaatagaacatatgaagcagaggaaaaagatcaatttggaaat
	atccagatagtcagaaaaaacataccggaaaatatatatcaggagctttataaatatttc
	aatgataaaagtgataaagaactttcggatgaagcagctaagcttaagaatgtagtaggt
	catcatgaggctgctacaaacatagtaaaagattatagatatacatatgataaatatttt
	cttcatatgcctattacaatcaattttaaagccaataagacaggctttattaatgacaga
	atattacaatatattgctaaagaaaaggatttgcatgtaataggcattgatcgtggtgaa
	agaaacctgatatatgtttcagtaattgatacttgtggaaatattgttgaacaaaaatcg
	tttaacattgttaatggatatgattatcagattaagctcaagcagcaggagggggcgcga
	caaatcgcacgaaaagaatggaaagaaatcggcaaaataaaagaaattaaagaaggctat
	ttatctcttgtaattcatgaaatttcaaagatggttattaaatataatgccataattgca
	atggaggatttaagctacggatttaaaaaaggtcgtttcaaggttgagcgacaggtttac
	cagaagtttgagacaatgcttatcaacaaactcaactatctggtatttaaagatatatcc
	ataacggaaaacggtggtcttctaaagggataccagcttacatatattccagataaactg
	aaaaatgtgggtcatcaatgtggctgtatattttatgtacctgctgcctatacatcaaaa
	atagatcctacaaccggatttgtaaatatattcaaatttaaagatttaacagttgatgcg
	aagagagaatttataaaaaaatttgacagtatcagatatgattcagaaaaaaatctgttt
	tgttttacattcgattataataactttattacgcaaaatactgttatgtcaaagtcaagc
	tggagtgtatatacgtacggagttaggataaaaagaagatttgtcaatggcaggttctca
	aatgaatcggatacaattgatataacaaaagatatggaaaaaacactcgaaatgacagat
	ataaattggagagatggtcatgatctgaggcaggatattattgattatgaaatcgtacaa
	cacatatttgagatttttagattgactgtacaaatgagaaacagtttaagtgaattagaa
	gacagggattatgaccgtttgatttctccggtgctcaatgaaaataatatattttatgat
	tcagctaaagcaggagatgcgttacctaaagacgcagatgctaatggtgcatattgtata
	gctctaaaaggcttgtatgaaatcaaacaaattacagagaattggaaagaagacggtaag
	ttttcaagagataaacttaaaatttccaataaggactggtttgactttattcaaaataaa
	aggtatttataa

SEQ	atgacaaacaaatttacaaaccagtactcgctttccaaaacacttcgatttgagttgatt
ID	ccacaaggaaaaacattggaatttattcaagaaaaaggattgctctctcaagataaacaa
NO:	cgagcggagagttatcaagaaatgaaaaaaactattgataaatttcataaatactttatc
28	gatttagctttaagcaatgctaaactaactcatttagaaacttacttggaattatacaat
	aaaagtgctgaaacaaaaaaagaacaaaaatttaaagacgatttaaagaaagtacaagac
	aatttacgaaaagaaatcgttaaatctttttcagatggtgatgcaaaatcaatttttgca
	attttggataaaaaagaactgattaccgtagaacttgaaaaatggtttgaaaacaacgaa
	caaaaagacatttattttgacgaaaaattcaaaacgtttactacttattttactggtttt
	catcaaaacagaaaaaacatgtattcggttgaacccaattctacagcaattgcttatcga
	ttgattcatgaaaatttacctaaatttttagaaaatgctaaagcatttgaaaaaataaaa
	caagtagaaagtttgcaagttaattttagagaattaatgggggaatttggagatgaaggg
	ctaattttcgtaaatgaattagaagaaatgtttcaaatcaattattataatgatgtgctt
	tcacaaaatggaattacaatttataatagtataatttcaggatttaccaaaaatgatata
	aaatataaaggtctaaatgaatacataaataattacaatcaaaccaaagacaaaaaagac
	cgtttgccaaaattaaaacaattgtataaacagattttgagtgataggatttcactttcg
	tttttgcccgatgcttttacggatgggaaacaagttttgaaagccatatttgacttttat
	aaaatcaacttactttcttataccattgaaggacaggaagaaagccaaaatcttttacta
	ttaattcgtcagacaattgaaaacctttctagttttgatacccaaaaaatttatctaaaa
	aatgatacccatttaaccactatttcacaacaagtatttggcgatttttcggtgttttca
	actgctttaaattattggtatgaaactaaagtaaatccaaaatttgaaacggaatatagc
	aaagccaacgaaaaaaaacgagaaattttagataaagccaaagcggtatttacaaaacaa
	gattatttttcaattgcttttttacaagaagtactttcggaatacattcttaccttagat
	cacacttctgatattgtaaaaaagcattcctccaactgtattgcggattattttaaaaat
	cattttgtagccaaaaaagaaaatgaaaccgacaaaacctttgattttattgctaatatt
	actgcaaaataccaatgtattcaaggtattttagaaaatgcagaccaatacgaagacgaa
	ctcaaacaagaccaaaaattaattgataatttgaaattctttttagatgctattttagaa
	ttgttgcattttattaaacctttgcatttaaaatcagaaagcattaccgaaaaagacact
	gctttttatgatgtgtttgaaaattattacgaagcattgagtttgttgaccccattatat
	aatatggtgcgaaactatgtaacgcaaaagccgtacagcaccgaaaaaataaaattaaat
	tttgaaaatgcacaattattgaatggttgggatgccaataaagaaggtgattacctaact
	accattttgaaaaaagacggtaattattttttagccataatggataaaaagcataacaaa
	gcgtttcaaaagtttccagaaggaaaagaaaattatgaaaaaatggtgtataaactattg
	cctggagtaaataagatgttgccaaaagtatttttttccaataaaaatattgcttacttc
	aacccatcaaaagagttattagaaaactataaaaaagagacgcacaaaaaaggagacaca
	ttcaatttagaacattgtcatacgttgatcgattttttcaaggactctttaaacaaacat
	gaagactggaaatactttgattttcaattttctgaaacaaaatcgtatcaagatttgagt
	ggtttttatagagaagtagaacatcaaggctacaaaatcaattttaaaaatatcgattca
	gaatatattgatggtttggtgaacgaaggtaaattgtttctatttcaaatttacagcaaa
	gatttttcgcctttttccaaagggaaaccgaacatgcacactttgtattggaaagcctta
	tttgaagaacaaaatttgcaaaatgtaatctataaattgaatggacaagccgaaatattt
	tttagaaaagcctctataaaacctaaaaatataatattgcacaaaaagaaaattaaaatt
	gccaaaaagcattttattgataaaaaaacaaaaacatctgaaattgttcctgttcaaaca
	ataaaaaacctcaatatgtactaccaaggaaaaataagtgaaaaagaattaacacaagat
	gatttaaggtatattgataattttagcattttcaatgaaaaaaataaaacaattgatatt
	ataaaagacaaacgatttacggttgataaatttcagtttcatgtgccgattaccatgaac
	tttaaagcaacgggcggaagttatatcaatcaaaccgtattagaatatttgcaaaacaat
	cccgaagttaagattattggattggatagaggcgaacgccatttggtatatctgacactg
	atagaccagcaaggaaacatcttgaaacaagaaagtttgaatacaatcaccgattctaaa
	atctcgacaccttatcataagttgttggataacaaggaaaacgagcgtgacttggctcga
	aaaaattggggaacggtggaaaacatcaaagaactcaaagaaggctacatcagtcaagtg
	gtgcataaaattgctacgttgatgctggaagaaaatgccattgtggtaatggaagatttg
	aattttggatttaaacgtggacgttttaaagtggaaaaacaaatttatcaaaagctggaa
	aaaatgttgattgacaaattgaattatttggttttaaaagacaaacaacctcaggaatta
	ggcggattgtacaacgcattacaactcaccaataaatttgaaagtttccaaaaaatgggt
	aaacaatcgggctttttgttttatgtacccgcttggaacacctccaaaatagacccaacc
	acagggtttgtcaattatttttataccaaatatgaaaatgttgacaaagccaaagccttt
	tttgaaaaatttgaggcgattcgtttcaatgcagaaaagaagtattttgaatttgaagta
	aaaaaatatagcgattttaacccaaaagccgaaggcactcaacaagcctggaccatttgc
	acgtatggcgaacgaatagaaaccaaacgacaaaaagaccaaaacaacaaatttgtaagc
	actccaattaatctaaccgaaaagatagaagactttttgggtaaaaaccaaattgtttat
	ggtgatggtaattgcatcaaatctcaaattgctagcaaagacgacaaggctttttttgaa
	accttattgtattggttcaaaatgactttacaaatgcgaaacagcgaaacaagaacagat
	atagattatctaatttcgcccgtgatgaatgacaacggaacattttacaacagccgagat
	tatgaaaaattagaaaatccaactttgcccaaagatgccgatgccaacggagcgtatcat
	attgccaaaaaaggattgatgcttttgaataaaatagaccaagccgacttgacaaaaaaa
	gtggatttatctattagtaacagagattggttgcaatttgtacaaaaaaataaataa

SEQ	atggaacaggagtactatttaggactggatatgggaaccggatctgtaggatgggctgtt
ID	acagattcggaatatcatgtcttgcgtaaacatggaaaagcactatggggagtccgatta
NO:	tttgaaagtgcatcgacagcagaagaacgaagaatgttccgaacatcaagaagaagacta
29	gatcgaagaaactggagaattgaaattttacaggaaatttttgcagaggaaataagtaag
	aaagatccaggatttttcttgcgaatgaaagaaagcaaatattatccagaagataagcga
	gatatcaatggaaattgtccggaactgccatatgcattatttgttgatgacgattttaca
	gataaagattatcataaaaaatttccgacaatttatcatctcaggaaaatgttgatgaat
	acagaggagacaccggatatccggttggtgtatctggcaattcatcatatgatgaagcat
	aggggccatttcttgttatctggtgacattaatgagattaaggagttcggaacgacattt
	tcaaaattgttggagaatatcaaaaatgaggaattggattggaatcttgaactgggaaaa
	gaagaatatgctgttgtagaaagtattttaaaagataacatgttaaaccgatccacaaag
	aaaaccagattaataaaagcattaaaagcaaaatcaatatgtgaaaaggctgtactgaat
	ttattggctggtggaacggtgaaattgagtgatatatttggtcttgaagaattaaatgag
	acagaaagaccgaagatttcctttgctgataatggatacgatgattatatcggagaagtt
	gaaaatgagctgggagaacaattctatattatagagacggcaaaagcagtgtatgactgg
	gcggtattagttgaaatattgggaaaatatacgtcaatttcagaagcgaaagtagcaacg
	tatgaaaaacataaatcggatttacaatttttgaaaaagatagttcggaaatatctgaca
	aaggaggaatataaagatatttttgtaagtacgagtgacaaattgaaaaattactctgct
	tatataggaatgacgaaaataaatggaaaaaaggttgatttgcagagcaaacggtgcagt
	aaagaagaattctatgattttattaagaaaaacgtacttaaaaagctagaaggacaacct
	gaatatgaatatttgaaagaagagctagaaagagaaacatttctaccaaaacaggtgaac
	agggataatggtgtaataccgtatcagattcatttgtacgagttgaaaaagatattagga
	aatttacgggataaaatagacctcattaaagagaacgaagataaactggttcaattattt
	gaattcagaattccgtattatgttggtccgctgaataagatagatgacggaaaagaggga
	aaatttacatgggctgtacggaaaagtaatgaaaagatatatccatggaattttgaaaat
	gtagttgatatagaagcaagtgcagaaaaatttatccggagaatgacaaataagtgtaca
	tatctgatgggcgaagatgtattgccgaaggattcattgctttacagtaaatatatggtt
	ttaaatgaattaaataatgtaaagttggatggcgaaaaattatctgtagaattgaaacaa
	cggttgtatacagatgtattttgtaagtatcggaaagtaactgtaaagaagataaaaaat
	tacttgaaatgtgaaggtatcatatccggcaatgtcgaaataactggaattgatggtgat
	tttaaggcatcgttaacggcatatcatgattttaaagaaatcttgacaggaacagaattg
	gctaaaaagga
	caaagaaaatattattaccaatatagtattgtttggagatgataaaaagctgctgaaaaa
	gagactgaatcgattatatcctcagattacgccgaatcagttgaagaaaatatgtgcgct
	atcctatacaggctggggaagattttctaaaaagttcttagaagaaataacagctccaga
	tccggaaacgggagaggtatggaatatcattacggcattgtgggaatcgaataataatct
	gatgcaattattaagtaatgaatatcggtttatggaagaagtcgaaacatacaatatggg
	aaaacagactaaaacattgtcgtacgaaacagtagagaatatgtatgtttctccatctgt
	gaaaagacagatatggcagacgctgaaaatcgtgaaagaattagaaaaagtaatgaaaga
	atctccgaaacgtgtatttattgagatggcgagagaaaagcaagaaagtaagagaaccga
	atcgcgtaaaaaacaactaatagatttgtataaggcttgtaaaaatgaagaaaaagattg
	ggtaaaagaactgggagatcaggaagaacagaaattacgaagcgataagttgtacctata
	ttatacgcaaaagggtcgttgtatgtattctggcgaggtaatagaactgaaagacttatg
	ggataatacaaaatatgatattgatcatatatatccacaatctaaaacgatggatgacag
	tcttaataatcgcgtattggtaaaaaagaaatataatgcaacaaaatcagataagtatcc
	attaaatgaaaatatacgacatgagagaaaaggcttttggaagtcactgttagatggagg
	gtttataagtaaagaaaaatatgaacgcttaataagaaatacagaattgagtccggaaga
	attagcaggatttattgaaaggcagattgttgaaacgaggcagagtacaaaagctgtagc
	ggaaatattaaagcaagtgtttccggaaagtgaaattgtatatgtcaaagcaggtacggt
	ttcaagattcagaaaagattttgaattactgaaagttcgagaagtgaatgatttgcatca
	cgcaaaggatgcgtatttaaatattgtagttggtaatagttattatgtgaaatttactaa
	gaatgcatcatggtttataaaagaaaatccgggacgtacttacaacttaaaaaagatgtt
	tacatcaggttggaatattgaacgaaatggagaagttgcatgggaagtcgggaaaaaagg
	aacaattgtaacggtaaaacaaataatgaataaaaataatatattggtgacaagacaggt
	tcatgaagcgaaaggtgggctgtttgatcagcagattatgaaaaaaggaaaaggtcagat
	tgctataaaggaaactgatgaacgtcttgcatcaatagaaaagtatggaggctataataa
	agctgccggggcatattttatgctggtagaatctaaagataaaaaaggaaaaacaattcg
	aacgatagaatttataccattatatttaaagaataaaatcgagtcggatgaatcaatagc
	attgaactttttagaaaaaggcagaggtttgaaagaaccaaagatactattgaaaaaaat
	taagattgatacattatttgatgtggacggattcaaaatgtggttgtctggaagaacagg
	ggacagactactatttaaatgtgcaaatcaattgattttggatgagaaaataattgtaac
	aatgaaaaaaattgtaaagtttattcaaaggagacaagaaaatagagaattaaaattatc
	tgataaagatggaattgataatgaagtacttatggaaatatataacacttttgtggataa
	gttagaaaacacagtgtatagaatacgattatccgaacaggcaaaaacgcttatagataa
	acaaaaagaatttgaaaggttatcactagaggataaaagtagtactttgtttgaaatttt
	acatatttttcagtgtcaaagtagtgcggccaatttaaaaatgataggcggacctggaaa
	agcaggaatattagttatgaataataatataagtaagtgtaacaaaatttctattataaa
	tcagtctccaacaggaattttcgaaaatgagattgatttgttaaagat

SEQ	ATGAAATCTTTCGATTCATTCACAAATCTTTATTCTCTTTCAAAAACCTTGAAATTTGAG
ID	ATGAGACCTGTCGGAAATACCCAAAAAATGCTCGACAATGCAGGAGTATTTGAAAAAGAC
NO:	AAACTAATTCAAAAAAAGTACGGAAAAACAAAGCCGTATTTCGACAGACTCCACAGAGAA
30	TTTATAGAAGAAGCGCTCACGGGGGTAGAGCTAATAGGACTAGATGAGAACTTTAGGACA
	CTTGTTGACTGGCAAAAAGATAAGAAAAATAATGTCGCAATGAAAGCGTATGAAAATAGT
	TTGCAGCGGCTGAGAACGGAAATAGGTAAAATATTTAACCTAAAGGCTGAGGATTGGGTA
	AAGAACAAATATCCAATATTAGGGCTGAAAAATAAAAATACCGATATTTTATTCGAAGAG
	GCTGTATTCGGGATATTGAAAGCCCGATATGGAGAAGAAAAAGATACTTTTATAGAAGTA
	GAGGAAATAGATAAAACCGGCAAATCAAAGATCAATCAAATATCAATTTTCGATAGTTGG
	AAAGGATTTACAGGATATTTCAAAAAATTTTTTGAAACCAGAAAGAATTTTTACAAAAAC
	GACGGAACTTCTACAGCAATTGCTACAAGGATCATTGATCAAAATCTGAAAAGATTCATA
	GATAATCTGTCAATAGTTGAAAGTGTGAGACAAAAGGTTGATCTCGCCGAGACAGAAAAA
	TCTTTCAGCATATCTCTATCGCAATTCTTCTCAATAGACTTTTATAACAAGTGTCTCCTT
	CAAGATGGTATTGATTACTACAACAAGATAATCGGTGGAGAAACTCTCAAAAATGGCGAA
	AAACTAATAGGTCTCAATGAACTAATAAATCAATATAGGCAGAATAATAAGGATCAGAAA
	ATCCCATTTTTCAAACTTCTTGATAAACAAATTTTGAGTGAAAAGATATTATTTTTGGAT
	GAAATAAAAAATGACACAGAACTGATCGAGGCGCTGAGTCAGTTCGCAAAAACAGCCGAA
	GAAAAAACAAAAATTGTCAAAAAGCTTTTTGCCGATTTTGTAGAAAATAATTCCAAATAC
	GATCTTGCACAGATTTATATTTCCCAAGAAGCATTCAATACTATATCAAACAAGTGGACA
	AGCGAAACTGAGACGTTCGCTAAATATCTATTCGAAGCAATGAAGAGTGGAAAACTTGCA
	AAGTATGAGAAAAAAGATAATAGCTATAAATTTCCTGATTTTATTGCCCTTTCACAGATG
	AAGAGTGCTTTATTAAGTATCAGCCTTGAGGGACATTTTTGGAAAGAGAAATACTACAAA
	ATTTCAAAATTCCAAGAGAAGACCAATTGGGAGCAGTTTCTTGCAATTTTTCTATACGAG
	TTTAACTCTCTTTTCAGCGACAAAATAAATACAAAAGATGGAGAAACAAAGCAAGTTGGA
	TACTATCTATTTGCCAAAGACCTGCATAATCTTATCTTAAGTGAGCAGATTGATATTCCA
	AAAGATTCAAAAGTCACAATAAAAGATTTTGCCGATTCTGTACTCACAATCTACCAAATG
	GCAAAATATTTTGCGGTAGAAAAAAAACGAGCGTGGCTTGCCGAGTATGAACTAGATTCA
	TTTTATACCCAGCCAGACACAGGCTATTTACAGTTTTATGATAACGCCTACGAGGATATT
	GTGCAGGTATACAACAAGCTTCGAAACTATCTGACCAAAAAGCCATATAGCGAGGAGAAA
	TGGAAGTTGAATTTTGAAAATTCTACGCTGGCAAATGGATGGGATAAGAACAAAGAATCT
	GATAATTCAGCAGTTATTCTACAAAAAGGTGGAAAATATTATTTGGGACTGATTACTAAA
	GGACACAACAAAATTTTTGATGACCGTTTTCAAGAAAAATTTATTGTGGGAATTGAAGGT
	GGAAAATATGAAAAAATAGTCTATAAATTTTTCCCCGACCAGGCAAAAATGTTTCCCAAA
	GTGTGCTTTTCTGCAAAAGGACTCGAATTTTTTAGACCGTCTGAAGAAATTTTAAGAATT
	TATAACAATGCAGAGTTTAAAAAAGGAGAAACTTATTCAATAGATAGTATGCAGAAGTTG
	ATTGATTTTTATAAAGATTGCTTGACTAAATATGAAGGCTGGGCATGTTATACCTTTCGG
	CATCTAAAACCCACAGAAGAATACCAAAACAATATTGGAGAGTTTTTTCGAGATGTTGCA
	GAGGACGGATACAGGATTGATTTTCAAGGCATTTCAGATCAATATATTCATGAAAAAAAC
	GAGAAAGGCGAACTTCACCTTTTTGAAATCCACAATAAAGATTGGAATTTGGATAAGGCA
	CGAGACGGAAAGTCAAAAACAACACAAAAAAACCTTCATACACTCTATTTCGAATCGCTC
	TTTTCAAACGATAATGTTGTTCAAAACTTTCCAATAAAACTCAATGGTCAAGCTGAAATT
	TTTTATAGACCGAAAACGGAAAAAGACAAATTAGAATCAAAAAAAGATAAGAAAGGGAAT
	AAAGTGATTGACCATAAACGCTATAGTGAGAATAAGATTTTTTTTCATGTTCCTCTCACA
	CTAAACCGCACTAAAAATGACTCATATCGCTTTAATGCTCAAATCAACAACTTTCTCGCA
	AATAATAAAGATATCAACATCATCGGTGTAGATAGGGGAGAAAAGCATTTAGTCTATTAT
	TCGGTGATTACACAAGCTAGTGACATCTTAGAAAGTGGCTCACTAAATGAGCTAAATGGC
	GTGAATTATGCTGAAAAACTGGGAAAAAAGGCAGAAAATCGAGAACAAGCACGCAGAGAC
	TGGCAAGACGTACAAGGGATCAAAGACCTCAAGAAAGGATATATTTCACAGGTGGTGCGA
	AAGCTTGCTGATTTAGCAATTAAACACAATGCCATTATCATTCTTGAAGATTTGAATATG
	AGATTTAAACAAGTTCGGGGCGGTATCGAAAAATCCATTTATCAACAGTTAGAAAAAGCA
	CTGATAGATAAATTAAGCTTTCTTGTAGACAAAGGTGAAAAAAATCCCGAGCAAGCAGGA
	CATCTTCTGAAAGCATATCAGCTTTCGGCCCCATTTGAGACATTTCAAAAAATGGGCAAA
	CAGACGGGTATAATCTTTTATACACAAGCTTCGTATACCTCAAAAAGTGACCCTGTAACA
	GGTTGGCGACCACACCTGTATCTCAAATATTTCAGTGCCAAAAAAGCAAAAGACGATATT
	GCAAAGTTTACAAAAATAGAATTTGTAAACGATAGGTTTGAGCTTACCTATGATATAAAG
	GACTTTCAGCAAGCAAAAGAATATCCAAATAAAACTGTTTGGAAAGTTTGCTCAAATGTA
	GAGAGATTCAGGTGGGACAAAAACCTCAATCAAAACAAAGGCGGATATACTCACTACACA
	AATATAACTGAGAATATCCAAGAGCTTTTTACAAAATATGGAATTGATATCACAAAAGAT
	TTGCTCACACAGATTTCTACAATTGATGAAAAACAAAATACCTCATTTTTTAGAGATTTT
	ATTTTTTATTTCAACCTTATTTGCCAAATCAGAAATACCGATGATTCTGAGATTGCTAAA
	AAGAATGGGAAAGATGATTTTATACTGTCACCTGTTGAGCCGTTTTTCGATAGCCGAAAA
	GACAATGGAAATAAACTTCCTGAGAATGGAGATGATAACGGCGCGTATAACATAGCAAGA
	AAAGGGATTGTCATACTCAACAAA
	ATCTCACAATATTCAGAGAAAAACGAAAATTGCGAGAAAATGAAATGGGGGGATTTGTAT
	GTATCAAACATTGACTGGGACAATTTTGTAACCCAAGCTAATGCACGGCATTAA

SEQ	ATGATTATCTTATATATTAGTACCTCGAATATGAACATGGAAGGAGTATTTATGGAAAAT
ID	TTTAAAAACTTGTATCCAATAAACAAAACACTTCGATTTGAATTAAGACCCTATGGAAAA
NO:	ACATTGGAAAATTTTAAAAAATCCGGACTTTTAGAAAAAGATGCCTTTAAGGCAAATAGT
31	AGACGAAGTATGCAAGCTATAATCGATGAAAAATTCAAAGAGACTATCGAAGAACGCTTA
	AAGTACACTGAATTCAGTGAATGTGATCTTGGAAACATGACATCAAAAGATAAAAAAATA
	ACTGATAAAGCAGCTACAAATTTAAAAAAGCAAGTTATCTTATCTTTTGACGATGAAATA
	TTTAATAATTACCTAAAACCTGATAAAAATATTGACGCATTATTTAAAAATGATCCTTCA
	AATCCTGTAATCTCTACATTTAAAGGTTTTACGACATATTTTGTGAATTTTTTTGAAATT
	CGAAAACATATTTTCAAGGGAGAATCATCAGGCTCAATGGCATACCGAATTATAGATGAA
	AACCTGACAACATACTTGAATAATATTGAAAAAATAAAAAAACTGCCAGAAGAATTAAAA
	TCACAGCTAGAAGGCATTGATCAGATTGATAAACTTAATAATTATAATGAGTTCATTACA
	CAGTCAGGTATAACACACTATAATGAAATCATCGGCGGTATATCAAAATCAGAGAATGTC
	AAAATACAGGGAATTAATGAAGGAATTAATCTATACTGTCAGAAGAACAAAGTTAAACTT
	CCTCGACTGACTCCGCTATACAAAATGATATTATCAGACAGAGTTTCCAACTCTTTTGTA
	TTAGACACTATTGAAAATGACACAGAATTAATTGAAATGATAAGTGATTTGATTAATAAG
	ACTGAGATTTCGCAAGATGTTATAATGTCAGATATTCAAAATATTTTCATAAAATACAAA
	CAACTTGGTAATTTGCCGGGTATCTCATATTCTTCAATAGTTAATGCTATTTGCTCGGAT
	TATGACAACAATTTCGGAGATGGGAAGCGAAAAAAATCTTACGAAAATGATCGCAAAAAG
	CATTTGGAGACTAATGTATACTCCATAAATTATATTTCTGAATTGCTTACAGATACCGAT
	GTTTCATCAAATATCAAGATGAGATATAAAGAGCTTGAGCAAAATTATCAGGTTTGCAAA
	GAAAATTTTAATGCCACAAACTGGATGAATATTAAAAATATAAAACAATCTGAAAAAACA
	AACCTTATTAAAGATTTGTTAGATATACTTAAATCGATTCAACGTTTCTATGATTTGTTT
	GATATTGTTGACGAAGATAAAAATCCAAGTGCTGAATTTTATACCTGGTTATCAAAAAAT
	GCTGAAAAGCTTGACTTTGAATTCAATTCTGTATATAACAAGTCACGAAACTATCTCACC
	AGGAAACAATACTCTGATAAAAAAATCAAGCTGAATTTTGATTCTCCAACATTGGCCAAA
	GGGTGGGATGCTAACAAAGAAATAGATAACTCCACGATTATAATGCGTAAATTTAATAAT
	GACAGAGGCGATTATGATTACTTCCTTGGCATATGGAATAAATCCACACCTGCAAATGAA
	AAAATAATCCCACTGGAGGATAATGGATTATTCGAAAAAATGCAATATAAGCTGTATCCA
	GATCCTAGTAAGATGTTACCGAAACAATTTCTATCAAAAATATGGAAGGCAAAGCATCCT
	ACGACACCTGAATTTGATAAAAAATATAAAGAGGGAAGACATAAAAAAGGTCCTGATTTC
	GAAAAAGAATTCCTGCATGAATTGATTGATTGCTTCAAACATGGTCTTGTTAATCACGAT
	GAAAAATATCAGGATGTTTTTGGCTTCAATCTCCGTAACACTGAAGATTATAATTCATAT
	ACAGAGTTTCTCGAAGATGTGGAAAGATGCAATTACAATCTTTCATTTAACAAAATTGCT
	GATACTTCAAACCTTATTAATGATGGGAAATTGTATGTATTTCAGATATGGTCAAAAGAC
	TTTTCTATTGATTCAAAAGGTACTAAAAACTTGAATACAATCTATTTTGAATCACTATTT
	TCAGAAGAAAACATGATAGAAAAAATGTTCAAGCTTTCTGGAGAGGCTGAGATATTCTAT
	CGACCAGCATCGTTGAATTATTGTGAAGATATCATAAAAAAAGGTCATCACCATGCAGAA
	TTAAAAGATAAGTTTGACTATCCTATAATAAAAGATAAGCGATATTCACAAGATAAGTTT
	TTCTTTCATGTGCCAATGGTTATAAATTATAAATCTGAGAAACTGAATTCCAAAAGCCTT
	AACAACCGAACAAATGAAAACCTGGGACAGTTTACACATATTATAGGTATAGACAGGGGC
	GAGCGGCACTTGATTTATTTAACTGTTGTTGATGTTTCCACTGGTGAAATCGTTGAACAG
	AAACATCTGGACGAAATTATCAATACTGATACCAAGGGAGTTGAACACAAAACCCATTAT
	TTGAATAAATTGGAAGAAAAATCTAAAACAAGAGATAACGAGCGTAAATCATGGGAAGCT
	ATTGAAACTATCAAAGAATTAAAAGAAGGCTATATTTCTCATGTAATTAATGAAATACAA
	AAGCTGCAAGAAAAATATAATGCCTTAATCGTAATGGAAAATCTTAACTATGGGTTCAAA
	AACTCACGAATCAAAGTTGAAAAACAGGTTTATCAAAAATTCGAGACAGCATTGATTAAA
	AAGTTCAATTATATTATTGATAAAAAAGATCCAGAAACCTATATACATGGTTACCAGCTT
	ACAAATCCTATTACCACTCTGGATAAGATTGGAAATCAATCTGGAATAGTGCTGTATATT
	CCTGCGTGGAATACTTCTAAGATAGATCCCGTCACAGGATTTGTAAACCTTCTGTACGCA
	GATGATTTGAAGTATAAAAATCAGGAGCAGGCCAAATCATTCATTCAGAAAATAGACAAC
	ATATATTTTGAAAATGGAGAGTTTAAATTTGATATTGATTTTTCCAAATGGAATAATCGC
	TACTCAATAAGTAAAACTAAATGGACGTTAACAAGTTATGGGACTCGCATCCAGACATTT
	AGAAATCCCCAGAAAAACAATAAGTGGGATTCTGCTGAATATGATTTGACAGAAGAGTTT
	AAATTAATTTTAAATATAGACGGAACGTTAAAGTCACAGGACGTAGAAACATACAAAAAA
	TTCATGTCTTTATTTAAACTAATGCTACAGCTTCGAAACTCTGTTACAGGAACCGACATT
	GATTATATGATCTCTCCTGTCACTGATAAAACAGGAACACATTTCGATTCAAGAGAAAAT
	ATTAAAAATCTTCCTGCCGATGCAGATGCCAATGGTGCCTACAACATTGCGCGCAAAGGA
	ATAATGGCTATTGAAAATATAATGAACGGTATAAGCGATCCACTAAAAATAAGCAACGAA
	GACTATTTAAAGTATATTCAGAATCAACAGGAATAA

SEQ	ATGACCCAATTTGAAGGTTTTACCAATTTATACCAAGTTTCGAAGACCCTTCGTTTTGAA
ID	CTGATTCCCCAAGGAAAAACACTCAAACATATCCAGGAGCAAGGGTTCATTGAGGAGGAT
NO:	AAAGCTCGCAATGACCATTACAAAGAGTTAAAACCAATCATTGACCGCATCTATAAGACT
32	TATGCTGATCAATGTCTCCAACTGGTACAGCTTGACTGGGAGAATCTATCTGCAGCCATA
	GACTCCTATCGTAAGGAAAAAACCGAAGAAACACGAAATGCGCTGATTGAGGAGCAAGCA
	ACATATAGAAATGCGATTCATGACTACTTTATAGGTCGGACGGATAATCTGACAGATGCC
	ATAAATAAGCGCCATGCTGAAATCTATAAAGGACTTTTTAAAGCTGAACTTTTCAATGGA
	AAAGTTTTAAAGCAATTAGGGACCGTAACCACGACAGAACATGAAAATGCTCTACTCCGT
	TCGTTTGACAAATTTACGACCTATTTTTCCGGCTTTTATGAAAACCGAAAAAATGTCTTT
	AGCGCTGAAGATATCAGCACGGCAATTCCCCATCGAATCGTCCAGGACAATTTCCCTAAA
	TTTAAGGAAAACTGCCATATTTTTACAAGATTGATAACCGCAGTTCCTTCTTTGCGGGAG
	CATTTTGAAAATGTCAAAAAGGCCATTGGAATCTTTGTTAGTACGTCTATTGAAGAAGTC
	TTTTCCTTTCCCTTTTATAATCAACTTCTAACCCAAACGCAAATTGATCTTTATAATCAA
	CTTCTCGGCGGCATATCTAGGGAAGCAGGCACAGAAAAAATCAAGGGACTTAATGAAGTT
	CTCAATCTGGCTATCCAAAAAAATGATGAAACAGCCCATATAATCGCGTCCCTGCCGCAT
	CGTTTTATTCCTCTTTTTAAACAAATTCTTTCCGATCGAAATACGTTATCCTTTATTTTG
	GAAGAATTCAAAAGCGATGAGGAAGTCATCCAATCCTTCTGCAAATATAAAACCCTCTTG
	AGAAACGAAAATGTACTGGAGACTGCAGAAGCCCTTTTCAATGAATTAAATTCCATTGAT
	TTGACTCATATCTTTATTTCCCATAAAAAGTTAGAAACCATCTCTTCAGCGCTTTGTGAC
	CATTGGGATACCTTGCGCAATGCACTTTACGAAAGACGGATTTCTGAACTCACTGGCAAA
	ATAACAAAAAGTGCCAAAGAAAAAGTTCAAAGGTCATTAAAACATGAGGATATAAATCTC
	CAAGAAATTATTTCTGCTGCAGGAAAAGAACTATCAGAAGCATTCAAACAAAAAACAAGT
	GAAATTCTTTCCCATGCCCATGCTGCACTTGACCAGCCTCTTCCCACAACATTAAAAAAA
	CAGGAAGAAAAAGAAATCCTCAAATCACAGCTCGATTCGCTTTTAGGCCTTTATCATCTT
	CTTGATTGGTTTGCTGTCGATGAAAGCAATGAAGTCGACCCAGAATTCTCAGCACGGCTG
	ACAGGCATTAAACTAGAAATGGAACCAAGCCTTTCGTTTTATAATAAAGCAAGAAATTAT
	GCGACAAAAAAGCCCTATTCGGTGGAAAAATTTAAATTGAATTTTCAAATGCCAACCCTT
	GCCTCTGGTTGGGATGTCAATAAAGAAAAAAATAATGGAGCTATTTTATTCGTAAAAAAT
	GGTCTCTATTACCTTGGTATCATGCCTAAACAGAAGGGGCGCTATAAAGCCCTGTCTTTT
	GAGCCGACAGAAAAAACATCAGAAGGATTCGATAAGATGTACTATGACTACTTCCCAGAT
	GCCGCAAAAATGATTCCTAAGTGTTCCACTCAGCTAAAGGCTGTAACCGCTCATTTTCAA
	ACTCATACCACCCCCATTCTTCTCTCAAATAATTTCATTGAACCTCTTGAAATCACAAAA
	GAAATTTATGACCTGAACAATCCTGAAAAGGAGCCTAAAAAGTTTCAAACGGCTTATGCA
	AAGAAGACAGGCGATCAAAAAGGCTATAGAGAAGCGCTTTGCAAATGGATTGACTTTACG
	CGGGATTTTCTCTCTAAATATACGAAAACAACTTCAATCGATTTATCTTCACTCCGCCCT
	TCTTCGCAATATAAAGATTTAGGGGAATATTACGCCGAACTGAATCCGCTTCTCTATCAT
	ATCTCCTTCCAACGAATTGCTGAAAAGGAAATCATGGATGCTGTAGAAACGGGAAAATTG
	TATCTGTTCCAAATCTACAATAAGGATTTTGCGAAGGGCCATCACGGGAAACCAAATCTC
	CACACCCTGTATTGGACAGGTCTCTTCAGTCCTGAAAACCTTGCGAAAACCAGCATCAAA
	CTTAATGGTCAAGCAGAATTGTTCTATCGACCTAAAAGCCGCATGAAGCGGATGGCCCAT
	CGTCTTGGGGAAAAAATGCTGAACAAAAAACTAAAGGACCAGAAGACACCGATTCCAGAT
	ACCCTCTACCAAGAACTGTACGATTATGTCAACCACCGGCTAAGCCATGATCTTTCCGAT
	GAAGCAAGGGCCCTGCTTCCAAATGTTATCACCAAAGAAGTCTCCCATGAAATTATAAAG
	GATCGGCGGTTTACTTCCGATAAATTTTTCTTCCATGTTCCCATTACACTGAATTATCAA
	GCAGCCAATAGTCCCAGTAAATTCAACCAGCGTGTCAATGCCTACCTTAAGGAGCATCCG
	GAAACGCCCATCATTGGTATCGATCGTGGAGAACGCAATCTAATCTATATTACCGTCATT
	GACAGTACTGGGAAAATTTTGGAGCAGCGTTCCCTGAATACCATCCAGCAATTTGACTAC
	CAAAAAAAATTGGACAACAGGGAAAAAGAGCGTGTTGCCGCCCGTCAAGCCTGGTCCGTC
	GTCGGAACGATCAAAGACCTTAAACAAGGCTACTTGTCACAGGTCATCCATGAAATTGTA
	GACCTGATGATTCATTACCAAGCTGTTGTCGTCCTTGAAAACCTCAACTTCGGATTTAAA
	TCAAAACGGACAGGCATTGCCGAAAAAGCAGTCTACCAACAATTTGAAAAGATGCTAATA
	GATAAACTCAACTGTTTGGTTCTCAAAGATTATCCTGCTGAGAAAGTGGGAGGCGTCTTA
	AACCCGTATCAACTTACAGATCAGTTCACGAGCTTTGCAAAAATGGGCACGCAAAGCGGC
	TTCCTTTTCTATGTACCGGCCCCTTATACCTCAAAGATTGATCCCCTGACTGGTTTTGTC
	GATCCCTTTGTATGGAAGACCATTAAAAATCATGAAAGTCGGAAGCATTTCCTAGAAGGA
	TTTGATTTCCTGCATTATGATGTCAAAACAGGTGATTTTATCCTCCATTTTAAAATGAAT
	CGGAATCTCTCTTTCCAGAGAGGGCTTCCTGGCTTCATGCCAGCTTGGGATATTGTTTTC
	GAAAAGAATGAAACCCAATTTGATGCAAAAGGGACGCCCTTCATTGCAGGAAAACGAATT
	GTTCCTGTAATCGAAAATCATCGTTTTACGGGTCGTTACAGAGACCTCTATCCCGCTAAT
	GAACTCATTGCCCTTCTGGAAGAAAAAGGCATTGTCTTTAGAGACGGAAGTAATATATTA
	CCCAAACTTTTAGAAAATGATGATTCTCATGCAATTGATACGATGGTCGCCTTGATTCGC
	AGTGTACTCCAAATGAGAAACAGCAATGCCGCAACGGGGGAAGACTACATCAACTCTCCC
	GTTAGGGATCTGAACGGGGTGTGTTTCGACAGTCGATTCCAAAATCCAGAATGGCCAATG
	GATGCGGATGCCAACGGAGCTTATCATATTGCCTTAAAAGGGCAGCTTCTTCTGAACCAC
	CTCAAAGAAAGCAAAGATCTGAAATTACAAAACGGCATCAGCAACCAAGATTGGCTGGCC
	TACATTCAGGAACTGAGAAACTGA

SEQ	ATGGCCGTCAAATCCATCAAAGTGAAACTTCGTCTCGACGATATGCCGGAGATTCGGGCC
ID	GGTCTATGGAAACTTCATAAGGAAGTCAATGCGGGGGTTCGATATTACACGGAATGGCTC
NO:	AGTCTTCTCCGTCAAGAGAACTTGTATCGAAGAAGTCCGAATGGGGACGGAGAGCAAGAA
33	TGTGATAAGACTGCAGAAGAATGCAAAGCCGAATTGTTGGAGCGGCTGCGCGCGCGTCAA
	GTGGAGAATGGACACCGTGGTCCGGCGGGATCGGACGATGAATTGCTGCAGTTGGCGCGT
	CAACTCTATGAGTTGTTGGTTCCGCAGGCGATAGGTGCGAAAGGCGACGCGCAGCAAATT
	GCCCGCAAATTTTTGAGCCCCTTGGCCGACAAGGACGCAGTTGGTGGGCTTGGAATCGCG
	AAGGCGGGGAACAAACCGCGGTGGGTTCGCATGCGCGAAGCGGGGGAACCAGGCTGGGAA
	GAGGAGAAGGAGAAGGCTGAGACGAGGAAATCTGCGGATCGGACTGCGGATGTTTTGCGC
	GCGCTCGCGGATTTTGGGTTAAAGCCACTGATGCGCGTATACACCGATTCTGAGATGTCA
	TCGGTGGAGTGGAAACCGCTTCGGAAGGGACAAGCCGTTCGGACGTGGGATAGGGACATG
	TTCCAACAAGCTATCGAACGGATGATGTCGTGGGAGTCGTGGAATCAGCGCGTTGGGCAA
	GAGTACGCGAAACTCGTAGAACAAAAAAATCGATTTGAGCAGAAGAATTTCGTCGGCCAG
	GAACATCTGGTCCATCTCGTCAATCAGTTGCAACAAGATATGAAAGAAGCATCGCCCGGA
	CTCGAATCGAAAGAGCAAACCGCGCACTATGTGACGGGACGGGCATTGCGCGGATCGGAC
	AAGGTATTTGAGAAGTGGGGGAAACTCGCCCCCGATGCACCTTTCGATTTGTACGACGCC
	GAAATCAAGAATGTGCAGAGACGTAACACGAGACGATTCGGATCACATGACTTGTTCGCA
	AAATTGGCAGAGCCAGAGTATCAGGCCCTGTGGCGCGAAGATGCTTCGTTTCTCACGCGT
	TACGCGGTGTACAACAGCATCCTTCGCAAACTGAATCACGCCAAAATGTTCGCGACGTTT
	ACTTTGCCGGATGCAACGGCGCACCCGATTTGGACTCGCTTCGATAAATTGGGTGGGAAT
	TTGCACCAGTACACCTTTTTGTTCAACGAATTTGGAGAACGCAGGCACGCGATTCGTTTT
	CACAAGCTATTGAAAGTCGAGAATGGTGTCGCAAGAGAAGTTGATGATGTCACCGTGCCC
	ATTTCAATGTCAGAGCAATTGGATAATCTGCTTCCCAGAGATCCCAATGAACCGATTGCG
	CTATATTTTCGAGATTACGGAGCCGAACAGCATTTCACAGGTGAATTTGGTGGCGCGAAG
	ATCCAGTGCCGCCGGGATCAGCTGGCTCATATGCACCGACGCAGAGGGGCGAGGGATGTT
	TATCTCAATGTCAGCGTACGTGTGCAGAGTCAGTCTGAGGCGCGGGGAGAACGTCGCCCG
	CCGTATGCGGCAGTATTTCGTCTGGTCGGGGACAACCATCGCGCGTTTGTCCATTTCGAT
	AAACTATCGGATTATCTTGCGGAACATCCGGATGATGGGAAGCTCGGGTCGGAGGGGTTG
	CTTTCCGGGCTGCGGGTGATGAGTGTCGATCTCGGCCTTCGCACATCTGCATCGATTTCC
	GTTTTTCGCGTTGCCCGGAAGGACGAGTTGAAGCCGAACTCAAAAGGTCGTGTACCGTTT
	TTCTTTCCGATAAAAGGGAATGACAATCTCGTCGCGGTTCATGAGCGATCACAACTCTTG
	AAGCTGCCTGGCGAAACGGAGTCGAAGGACCTGCGTGCTATCCGAGAAGAACGCCAACGG
	ACATTGCGGCAGTTGCGGACGCAACTGGCGTATTTGCGGCTGCTCGTGCGGTGTGGGTCG
	GAAGATGTGGGGCGGCGTGAACGGAGTTGGGCAAAGCTTATCGAGCAGCCGGTGGATGCG
	GCCAATCACATGACACCGGATTGGCGCGAGGCTTTTGAAAACGAACTTCAGAAGCTTAAG
	TCACTCCATGGTATCTGTAGCGACAAGGAATGGATGGATGCTGTCTACGAGAGCGTTCGC
	CGCGTGTGGCGTCACATGGGCAAACAGGTTCGCGATTGGCGAAAGGACGTACGAAGCGGA
	GAGCGGCCCAAGATTCGCGGCTATGCGAAAGACGTGGTCGGTGGAAACTCGATTGAGCAA
	ATCGAGTATCTGGAACGTCAGTACAAGTTCCTCAAGAGTTGGAGCTTCTTTGGTAAGGTG
	TCGGGACAAGTGATTCGTGCGGAGAAGGGATCTCGTTTTGCGATCACGCTGCGCGAACAC
	ATTGATCACGCGAAGGAAGATCGGCTGAAGAAATTGGCGGATCGCATCATTATGGAGGCT
	CTCGGCTATGTGTACGCGTTGGATGAGCGCGGCAAAGGAAAGTGGGTTGCGAAGTATCCG
	CCGTGCCAGCTCATCCTGCTGGAGGAATTGAGCGAGTACCAGTTCAATAACGACAGGCCT
	CCGAGCGAAAACAACCAGTTGATGCAATGGAGTCATCGCGGCGTGTTCCAGGAGTTGATA
	AATCAGGCCCAAGTCCATGATTTACTCGTTGGGACGATGTATGCAGCGTTCTCGTCGCGA
	TTCGACGCGCGAACTGGGGCACCGGGTATCCGCTGTCGCCGGGTTCCGGCGCGTTGCACC
	CAGGAGCACAATCCAGAACCATTTCCTTGGTGGCTGAACAAGTTTGTGGTGGAACATACG
	TTGGATGCTTGTCCCCTACGCGCAGACGACCTCATCCCAACGGGTGAAGGAGAGATTTTT
	GTCTCGCCGTTCAGCGCGGAGGAGGGGGACTTTCATCAGATTCACGCCGACCTGAATGCG
	GCGCAAAATCTGCAGCAGCGACTCTGGTCTGATTTTGATATCAGTCAAATTCGGTTGCGG
	TGTGATTGGGGTGAAGTGGACGGTGAACTCGTTCTGATCCCAAGGCTTACAGGAAAACGA
	ACGGCGGATTCATATAGCAACAAGGTGTTTTATACCAATACAGGTGTCACCTATTATGAG
	CGAGAGCGGGGGAAGAAGCGGAGAAAGGTTTTCGCGCAAGAGAAATTGTCGGAGGAAGAG
	GCGGAGTTGCTCGTGGAAGCAGACGAGGCGAGGGAGAAATCGGTCGTTTTGATGCGTGAT
	CCGTCTGGCATCATCAATCGGGGAAATTGGACCAGGCAAAAGGAATTTTGGTCGATGGTG
	AACCAGCGGATCGAAGGATACTTGGTCAAGCAGATTCGCTCGCGCGTTCCATTACAAGAT
	AGTGCGTGTGAAAACACGGGGGATATTTAA

SEQ	ATGGCGACACGCAGTTTTATTTTAAAAATTGAACCAAATGAAGAAGTTAAAAAGGGATTA
ID	TGGAAGACGCATGAGGTATTGAATCATGGAATTGCCTACTACATGAATATTCTGAAACTA
NO:	ATTAGACAGGAAGCTATTTATGAACATCATGAACAAGATCCTAAAAATCCGAAAAAAGTT
34	TCAAAAGCAGAAATACAAGCCGAGTTATGGGATTTTGTTTTAAAAATGCAAAAATGTAAT
	AGTTTTACACATGAAGTTGACAAAGATGTTGTTTTTAACATCCTGCGTGAACTATATGAA
	GAGTTGGTCCCTAGTTCAGTCGAGAAAAAGGGTGAAGCCAATCAATTATCGAATAAGTTT
	CTGTACCCGCTAGTTGATCCGAACAGTCAAAGTGGGAAAGGGACGGCATCATCCGGACGT
	AAACCTCGGTGGTATAATTTAAAAATAGCAGGCGACCCATCGTGGGAGGAAGAAAAGAAA
	AAATGGGAAGAGGATAAAAAGAAAGATCCCCTTGCTAAAATCTTAGGTAAGTTAGCAGAA
	TATGGGCTTATTCCGCTATTTATTCCATTTACTGACAGCAACGAACCAATTGTAAAAGAA
	ATTAAATGGATGGAAAAAAGTCGTAATCAAAGTGTCCGGCGACTTGATAAGGATATGTTT
	ATCCAAGCATTAGAGCGTTTTCTTTCATGGGAAAGCTGGAACCTTAAAGTAAAGGAAGAG
	TATGAAAAAGTTGAAAAGGAACACAAAACACTAGAGGAAAGGATAAAAGAGGACATTCAA
	GCATTTAAATCCCTTGAACAATATGAAAAAGAACGGCAGGAGCAACTTCTTAGAGATACA
	TTGAATACAAATGAATACCGATTAAGCAAAAGAGGATTACGTGGTTGGCGTGAAATTATC
	CAAAAATGGCTAAAGATGGATGAAAATGAACCATCAGAAAAATATTTAGAAGTATTTAAA
	GATTATCAACGGAAACATCCACGAGAAGCCGGGGACTATTCTGTCTATGAATTTTTAAGC
	AAGAAAGAAAATCATTTTATTTGGCGAAATCATCCTGAATATCCTTATTTGTATGCTACA
	TTTTGTGAAATTGACAAAAAAAAGAAAGACGCTAAGCAACAGGCAACTTTTACTTTGGCT
	GACCCGATTAACCATCCGTTATGGGTACGATTTGAAGAAAGAAGCGGTTCGAACTTAAAC
	AAATATCGAATTTTAACAGAGCAATTACACACTGAAAAGTTAAAAAAGAAATTAACAGTT
	CAACTTGATCGTTTAATTTATCCAACTGAATCCGGCGGTTGGGAGGAAAAAGGTAAAGTA
	GATATCGTTTTGTTGCCGTCAAGACAATTTTATAATCAAATCTTCCTTGATATAGAAGAA
	AAGGGGAAACATGCTTTTACTTATAAGGATGAAAGTATTAAATTCCCCCTTAAAGGTACA
	CTTGGTGGTGCAAGAGTGCAGTTTGACCGTGACCATTTGCGGAGATATCCGCATAAAGTA
	GAATCAGGAAATGTTGGACGGATTTATTTTAACATGACAGTAAATATTGAACCAACTGAG
	AGCCCTGTTAGTAAGTCTTTGAAAATACATAGGGACGATTTCCCCAAGTTCGTTAATTTT
	AAACCGAAAGAGCTCACCGAATGGATAAAAGATAGTAAAGGGAAAAAATTAAAAAGTGGT
	ATAGAATCCCTTGAAATTGGTCTACGGGTGATGAGTATCGACTTAGGTCAACGTCAAGCG
	GCTGCTGCATCGATTTTTGAAGTAGTTGATCAGAAACCGGATATTGAAGGGAAGTTATTT
	TTTCCAATCAAAGGAACTGAGCTTTATGCTGTTCACCGGGCAAGTTTTAACATTAAATTA
	CCGGGTGAAACATTAGTAAAATCACGGGAAGTATTGCGGAAAGCTCGGGAGGACAACTTA
	AAATTAATGAATCAAAAGTTAAACTTTCTAAGAAATGTTCTACATTTCCAACAGTTTGAA
	GATATCACAGAAAGAGAGAAGCGTGTAACTAAATGGATTTCTAGACAAGAAAATAGTGAT
	GTTCCTCTTGTATATCAAGATGAGCTAATTCAAATTCGTGAATTAATGTATAAACCCTAT
	AAAGATTGGGTTGCCTTTTTAAAACAACTCCATAAACGGCTAGAAGTCGAGATTGGCAAA
	GAGGTTAAGCATTGGCGAAAATCATTAAGTGACGGGAGAAAAGGTCTTTACGGAATCTCC
	CTAAAAAATATTGATGAAATTGATCGAACAAGGAAATTCCTTTTAAGATGGAGCTTACGT
	CCAACAGAACCTGGGGAAGTAAGACGCTTGGAACCAGGACAGCGTTTTGCGATTGATCAA
	TTAAACCACCTAAATGCATTAAAAGAAGATCGATTAAAAAAGATGGCAAATACGATTATC
	ATGCATGCCTTAGGTTACTGTTATGATGTAAGAAAGAAAAAGTGGCAGGCAAAAAATCCA
	GCATGTCAAATTATTTTATTTGAAGATTTATCTAACTACAATCCTTACGAGGAAAGGTCC
	CGTTTTGAAAACTCAAAACTGATGAAGTGGTCACGGAGAGAAATTCCACGACAAGTCGCC
	TTACAAGGTGAAATTTACGGATTACAAGTTGGGGAAGTAGGTGCCCAATTCAGTTCAAGA
	TTCCATGCGAAAACCGGGTCGCCGGGAATTCGTTGCAGTGTTGTAACGAAAGAAAAATTG
	CAGGATAATCGCTTTTTTAAAAATTTACAAAGAGAAGGACGACTTACTCTTGATAAAATC
	GCAGTTTTAAAAGAAGGAGACTTATATCCAGATAAAGGTGGAGAAAAGTTTATTTCTTTA
	TCAAAGGATCGAAAGTTGGTAACTACGCATGCTGATATTAACGCGGCCCAAAATTTACAG
	AAGCGTTTTTGGACAAGAACACATGGATTTTATAAAGTTTACTGCAAAGCCTATCAGGTT
	GATGGACAAACTGTTTATATTCCGGAGAGCAAGGACCAAAAACAAAAAATAATTGAAGAA
	TTTGGGGAAGGCTATTTTATTTTAAAAGATGGTGTATATGAATGGGGTAATGCGGGGAAA
	CTAAAAATTAAAAAAGGTTCCTCTAAACAATCATCGAGTGAATTAGTAGATTCGGACATA
	CTGAAAGATTCATTTGATTTAGCAAGTGAACTTAAGGGAGAGAAACTCATGTTATATCGA
	GATCCGAGTGGAAACGTATTTCCTTCCGACAAGTGGATGGCAGCAGGAGTATTTTTTGGC
	AAATTAGAAAGAATATTGATTTCTAAGTTAACAAATCAATACTCAATATCAACAATAGAA
	GATGATTCTTCAAAACAATCAATGTAA

SEQ	ATGCCCACCCGCACCATCAATCTGAAACTTGTTCTTGGGAAAAATCCTGAAAACGCAACA
ID	TTGCGACGCGCCCTATTTTCGACACACCGTTTGGTTAACCAAGCGACGAAACGTATTGAG
NO:	GAATTCTTGTTGCTGTGTCGTGGAGAAGCCTACAGAACAGTGGATAATGAGGGGAAGGAA
35	GCCGAGATTCCACGTCATGCAGTCCAAGAAGAAGCTCTTGCCTTTGCCAAAGCTGCTCAA
	CGCCACAACGGCTGTATATCCACCTATGAAGACCAAGAGATTCTTGATGTACTGCGGCAA
	CTGTACGAACGTCTTGTTCCTTCGGTCAACGAAAACAACGAGGCAGGCGATGCTCAAGCT
	GCTAACGCCTGGGTCAGTCCGCTCATGTCGGCAGAAAGCGAAGGAGGCTTGTCGGTCTAC
	GACAAGGTGCTTGATCCACCGCCGGTTTGGATGAAGCTTAAAGAAGAAAAGGCTCCAGGA
	TGGGAAGCCGCTTCTCAAATTTGGATTCAGAGTGATGAGGGACAGTCGTTACTTAATAAG
	CCAGGTAGCCCTCCCCGCTGGATTCGAAAACTGCGATCTGGGCAACCGTGGCAAGATGAT
	TTCGTCAGTGACCAAAAGAAAAAGCAAGATGAGCTGACCAAAGGGAACGCACCACTTATA
	AAACAACTCAAAGAAATGGGGTTGTTGCCTCTTGTTAACCCATTTTTTAGACATCTTCTT
	GACCCTGAAGGTAAAGGCGTGAGTCCATGGGACCGTCTTGCTGTACGCGCTGCAGTGGCT
	CACTTTATCTCCTGGGAAAGTTGGAATCATAGAACACGTGCAGAATACAATTCCTTGAAA
	CTACGGCGAGACGAGTTTGAGGCAGCATCCGACGAATTCAAAGACGATTTTACTTTGCTC
	CGACAATATGAAGCCAAACGCCATAGTACATTGAAAAGCATCGCGCTGGCCGACGATTCG
	AACCCTTACCGGATTGGAGTACGTTCTCTGCGTGCCTGGAACCGCGTTCGTGAAGAATGG
	ATAGACAAGGGTGCAACAGAAGAACAACGCGTGACCATATTGTCAAAGCTTCAAACACAA
	CTTCGGGGAAAATTCGGCGATCCCGATCTGTTCAACTGGCTAGCTCAGGATAGGCATGTC
	CATTTGTGGTCTCCTCGGGACAGCGTGACACCATTGGTTCGCATCAATGCGGTAGATAAA
	GTTCTGCGTCGACGAAAACCGTATGCATTGATGACCTTTGCCCATCCCCGCTTCCACCCT
	CGATGGATACTGTACGAGGCTCCAGGAGGAAGCAATCTCCGTCAATATGCATTGGATTGT
	ACAGAAAACGCTCTACACATCACGTTGCCTTTGCTTGTCGACGATGCGCACGGAACCTGG
	ATTGAAAAAAAGATCAGGGTGCCGCTGGCACCATCCGGACAAATTCAAGATTTAACTCTG
	GAAAAACTTGAGAAGAAAAAAAATCGTTTATACTACCGTTCCGGTTTTCAGCAGTTTGCC
	GGCTTGGCTGGCGGAGCTGAGGTTCTTTTCCACAGACCCTATATGGAACACGACGAACGC
	AGCGAGGAGTCTCTTTTGGAACGTCCGGGAGCCGTTTGGTTCAAATTGACCCTGGATGTG
	GCAACACAGGCTCCCCCGAACTGGCTTGATGGTAAGGGCCGTGTCCGTACACCGCCGGAG
	GTACATCATTTTAAAACCGCATTGTCGAATAAAAGCAAACATACACGTACGCTGCAGCCG
	GGTCTCCGTGTCTTGTCAGTAGACTTGGGCATGCGAACATTCGCCTCCTGCTCAGTATTT
	GAACTCATCGAGGGAAAGCCTGAGACAGGCCGTGCCTTCCCTGTTGCCGATGAGAGATCA
	ATGGACAGCCCGAATAAACTGTGGGCCAAGCATGAACGTAGTTTTAAACTGACGCTCCCC
	GGCGAAACCCCTTCTCGAAAGGAAGAGGAAGAGCGTAGCATAGCAAGAGCGGAAATTTAT
	GCACTGAAACGCGACATACAACGCCTCAAAAGCCTACTCCGCTTAGGTGAAGAAGATAAC
	GATAACCGTCGTGATGCATTGCTTGAACAGTTCTTTAAAGGATGGGGAGAAGAAGACGTT
	GTGCCTGGACAAGCGTTTCCACGCTCTCTTTTCCAAGGGTTGGGAGCTGCCCCGTTTCGC
	TCAACTCCAGAGTTATGGCGTCAGCATTGCCAAACATATTATGACAAAGCGGAAGCCTGT
	CTGGCTAAACATATCAGTGATTGGCGCAAGCGAACTCGTCCCCGTCCGACATCGCGGGAG
	ATGTGGTACAAAACACGTTCCTATCATGGCGGCAAGTCCATTTGGATGTTGGAATATCTT
	GATGCCGTTCGAAAACTGCTTCTCAGTTGGAGCTTACGTGGTCGTACTTACGGTGCCATT
	AATCGCCAGGATACAGCCCGGTTTGGTTCTTTGGCATCACGGCTGCTCCACCATATCAAT
	TCCCTAAAGGAAGACCGCATCAAAACAGGAGCCGACTCTATCGTTCAGGCTGCTCGCGGG
	TATATTCCTCTCCCTCATGGCAAGGGTTGGGAACAAAGATATGAGCCTTGTCAGCTCATA
	TTATTTGAAGACCTCGCACGATATCGCTTTCGCGTGGATCGACCTCGTCGAGAGAACAGC
	CAACTCATGCAGTGGAACCATCGAGCCATCGTGGCAGAAACAACGATGCAAGCCGAACTC
	TACGGACAAATTGTCGAAAATACTGCAGCGGGGTTCAGCAGTCGTTTTCACGCGGCGACA
	GGTGCCCCCGGTGTACGTTGTCGTTTTCTTCTAGAAAGAGACTTTGATAACGATTTGCCC
	AAACCGTACCTTCTCAGGGAACTTTCTTGGATGCTCGGCAATACAAAAGTCGAGTCTGAA
	GAAGAAAAGCTTCGATTGCTGTCTGAAAAAATCAGGCCAGGCAGTCTTGTTCCTTGGGAT
	GGAGGCGAACAGTTCGCTACCCTGCATCCCAAAAGACAAACACTTTGCGTCATTCATGCC
	GATATGAATGCTGCCCAAAATTTACAACGCCGGTTTTTCGGTCGATGCGGCGAGGCCTTT
	CGGCTTGTTTGTCAACCCCACGGTGACGACGTGTTACGACTCGCATCCACCCCAGGAGCT
	CGTCTTCTTGGAGCCCTGCAGCAGCTTGAAAATGGACAAGGAGCTTTCGAGTTGGTTCGA
	GACATGGGGTCAACAAGTCAAATGAACCGGTTCGTCATGAAGTCTTTGGGAAAAAAGAAA
	ATAAAACCCCTTCAGGACAACAATGGAGACGACGAGCTTGAAGACGTGTTGTCCGTACTC
	CCGGAGGAAGACGACACAGGACGTATCACAGTCTTCCGCGATTCATCAGGAATCTTTTTT
	CCTTGCAACGTCTGGATACCGGCCAAACAGTTTTGGCCAGCAGTACGCGCCATGATTTGG
	AAGGTCATGGCTTCCCATTCTTTGGGGTGA

SEQ	ATGACAAAGTTAAGACACCGACAGAAAAAATTAACACACGACTGGGCTGGCTCCAAAAAG
ID	AGGGAAGTATTAGGCTCAAATGGCAAGCTTCAGAATCCGTTGTTAATGCCGGTTAAAAAA
NO:	GGTCAGGTTACTGAGTTCCGGAAAGCGTTTTCTGCGTATGCTCGCGCAACGAAAGGAGAA
36	ATGACTGACGGCCGAAAGAATATGTTTACGCATAGTTTCGAGCCATTTAAGACAAAGCCC
	TCGCTTCATCAGTGTGAATTGGCAGATAAAGCATATCAATCTTTACATTCGTATCTGCCT
	GGTTCTCTTGCTCATTTTCTATTATCTGCTCACGCATTAGGTTTTCGTATTTTTTCAAAA
	TCTGGTGAAGCAACTGCATTCCAGGCATCCTCTAAAATTGAAGCTTACGAATCAAAATTG
	GCAAGCGAATTAGCTTGTGTAGATTTATCTATTCAAAACTTGACTATTTCAACGCTTTTT
	AATGCGCTTACAACGTCTGTAAGAGGGAAGGGCGAAGAAACTAGCGCTGACCCCTTAATT
	GCACGATTTTACACCTTACTTACTGGCAAGCCTCTGTCTCGAGACACTCAAGGGCCTGAA
	CGTGATTTAGCAGAAGTTATCTCGCGTAAGATAGCTAGTTCTTTTGGCACATGGAAAGAA
	ATGACGGCAAACCCTCTTCAGTCATTACAATTTTTTGAAGAGGAACTCCATGCGCTGGAT
	GCCAATGTCTCGCTCTCACCCGCCTTCGACGTTTTAATTAAAATGAATGATTTGCAGGGC
	GATTTAAAAAATCGAACCATTGTTTTTGATCCTGACGCCCCTGTTTTTGAATATAACGCA
	GAAGACCCTGCCGACATAATTATTAAACTTACAGCTCGTTACGCTAAAGAAGCTGTCATC
	AAAAATCAAAACGTAGGAAATTACGTTAAAAACGCTATTACTACCACAAATGCCAATGGT
	CTTGGTTGGCTTTTGAACAAAGGTTTGTCGTTACTCCCTGTCTCGACCGATGACGAATTG
	CTAGAGTTTATTGGCGTTGAACGATCTCATCCCTCATGCCATGCCTTAATTGAATTGATT
	GCACAATTAGAAGCCCCCGAGCTCTTTGAGAAGAACGTATTTTCAGATACTCGTTCTGAA
	GTTCAAGGTATGATTGATTCAGCTGTTTCTAATCATATTGCTCGTCTTTCCAGCTCTAGA
	AATAGCTTGTCAATGGATAGTGAAGAATTAGAACGTTTAATCAAAAGCTTTCAGATACAC
	ACACCTCATTGCTCACTTTTTATTGGCGCCCAATCACTTTCACAGCAGTTAGAATCTTTG
	CCTGAAGCCCTTCAATCGGGCGTTAATTCAGCCGATATTTTACTAGGCTCTACTCAATAT
	ATGCTCACCAATTCTTTGGTTGAAGAGTCAATTGCAACTTATCAAAGAACACTTAATCGC
	ATCAATTACTTGTCAGGTGTTGCAGGTCAGATTAACGGCGCAATAAAGCGAAAAGCGATA
	GATGGAGAAAAAATTCACTTGCCTGCAGCTTGGTCAGAGTTGATATCTTTACCATTTATA
	GGCCAGCCTGTTATAGATGTTGAAAGCGATTTAGCTCATCTAAAAAATCAATACCAAACA
	CTTTCAAATGAGTTTGATACTCTTATATCTGCTTTGCAAAAGAATTTTGATTTGAACTTT
	AATAAAGCGCTCCTTAATCGTACTCAGCATTTTGAAGCCATGTGTAGAAGCACTAAGAAA
	AACGCTTTATCCAAACCAGAGATCGTTTCCTATCGCGACCTGCTTGCTCGATTAACTTCT
	TGTTTGTATCGAGGCTCTTTAGTTTTGCGTCGTGCCGGCATTGAAGTGTTAAAAAAACAT
	AAAATATTTGAGTCAAACAGCGAACTTCGTGAACATGTTCATGAAAGAAAGCATTTCGTG
	TTTGTTAGTCCTCTAGATCGCAAAGCCAAGAAACTCCTTCGATTAACTGATTCGCGTCCA
	GACTTGTTACATGTTATTGATGAAATATTGCAGCACGATAATCTTGAAAACAAAGACCGC
	GAGTCACTTTGGCTAGTTCGCTCTGGTTATTTGCTTGCAGGACTTCCAGATCAACTTTCT
	TCATCTTTTATTAACTTGCCTATCATTACTCAAAAAGGAGATAGACGCCTTATAGACCTG
	ATTCAGTATGATCAAATTAATCGTGATGCTTTTGTTATGTTAGTGACCTCTGCATTCAAG
	TCTAATTTGTCTGGTCTGCAGTATCGTGCCAATAAGCAATCGTTCGTTGTTACTCGCACG
	CTAAGCCCTTATCTCGGCTCAAAACTTGTCTACGTACCCAAGGATAAAGATTGGTTAGTT
	CCTTCTCAAATGTTTGAAGGACGATTTGCTGACATTCTTCAATCAGATTATATGGTCTGG
	AAAGATGCCGGTCGTCTTTGTGTTATTGATACTGCAAAACACCTTTCTAATATAAAGAAG
	TCTGTATTTTCATCCGAAGAAGTTCTCGCTTTTTTAAGAGAACTCCCTCACCGCACATTT
	ATCCAGACCGAAGTTCGCGGCCTTGGCGTTAATGTCGATGGAATTGCATTTAATAATGGT
	GATATTCCGTCATTAAAAACCTTTTCAAATTGCGTTCAGGTAAAAGTTTCTCGGACTAAT
	ACATCCCTAGTTCAAACACTTAATCGTTGGTTTGAAGGAGGAAAAGTTTCTCCTCCGAGC
	ATTCAATTTGAACGGGCGTATTATAAAAAAGACGATCAAATTCATGAAGACGCAGCGAAA
	AGAAAGATACGATTCCAGATGCCCGCAACTGAGTTGGTTCATGCTTCTGACGATGCGGGG
	TGGACACCAAGTTATTTGCTCGGCATTGATCCTGGCGAGTATGGAATGGGTCTTTCATTG
	GTTTCGATTAATAACGGAGAAGTCTTAGATTCAGGCTTTATTCATATTAATTCTCTGATC
	AATTTTGCCTCTAAAAAGAGCAACCATCAAACTAAGGTTGTTCCGCGTCAGCAGTACAAA
	TCTCCTTATGCAAATTATTTAGAACAATCTAAAGATTCTGCTGCTGGTGATATTGCGCAT
	ATACTCGATCGACTTATATACAAATTAAATGCGTTGCCTGTTTTTGAGGCTCTTTCAGGT
	AATTCTCAGAGTGCTGCTGATCAAGTTTGGACGAAAGTCTTATCGTTTTACACTTGGGGT
	GATAATGACGCTCAGAATTCTATTAGAAAGCAGCATTGGTTTGGAGCCAGTCATTGGGAT
	ATCAAAGGTATGTTAAGGCAACCCCCTACGGAGAAGAAGCCTAAACCGTATATTGCTTTT
	CCTGGCTCTCAGGTTTCTTCGTATGGTAATTCCCAACGTTGCTCTTGCTGCGGTCGCAAT
	CCTATTGAACAACTTCGAGAAATGGCAAAGGATACCTCTATTAAAGAGCTAAAAATTCGC
	AATTCTGAGATACAGCTTTTTGACGGAACCATTAAATTATTTAATCCAGACCCATCCACT
	GTGATAGAGAGAAGGCGACATAATCTTGGTCCATCAAGAATTCCTGTTGCTGACCGTACT
	TTCAAAAACATCAGTCCATCAAGTCTAGAATTTAAAGAATTGATTACTATCGTGTCTCGA
	TCTATCCGTCATTCACCTGAGTTTATCGCTAAAAAACGCGGCATAGGGTCTGAGTATTTT
	TGCGCTTATTCCGATTGCAACTCATCCTTAAATTCTGAAGCTAACGCAGCTGCTAACGTA
	GCGCAAAAATTTCAAAAACAGTTATTTTTTGAGTTATAA

SEQ	ATGAAGAGAATTCTGAACAGTCTGAAAGTTGCTGCCTTGAGACTTCTGTTTCGAGGCAAA
ID	GGTTCTGAATTAGTGAAGACAGTCAAATATCCATTGGTTTCCCCGGTTCAAGGCGCGGTT
NO:	GAAGAACTTGCTGAAGCAATTCGGCACGACAACCTGCACCTTTTTGGGCAGAAGGAAATA
37	GTGGATCTTATGGAGAAAGACGAAGGAACCCAGGTGTATTCGGTTGTGGATTTTTGGTTG
	GATACCCTGCGTTTAGGGATGTTTTTCTCACCATCAGCGAATGCGTTGAAAATCACGCTG
	GGAAAATTCAATTCTGATCAGGTTTCACCTTTTCGTAAGGTTTTGGAGCAGTCACCTTTT
	TTTCTTGCGGGTCGCTTGAAGGTTGAACCTGCGGAAAGGATACTTTCTGTTGAAATCAGA
	AAGATTGGTAAAAGAGAAAACAGAGTTGAGAACTATGCCGCCGATGTGGAGACATGCTTC
	ATTGGTCAGCTTTCTTCAGATGAGAAACAGAGTATCCAGAAGCTGGCAAATGATATCTGG
	GATAGCAAGGATCATGAGGAACAGAGAATGTTGAAGGCGGATTTTTTTGCTATACCTCTT
	ATAAAAGACCCCAAAGCTGTCACAGAAGAAGATCCTGAAAATGAAACGGCGGGAAAACAG
	AAACCGCTTGAATTATGTGTTTGTCTTGTTCCTGAGTTGTATACCCGAGGTTTCGGCTCC
	ATTGCTGATTTTCTGGTTCAGCGACTTACCTTGCTGCGTGACAAAATGAGTACCGACACG
	GCGGAAGATTGCCTCGAGTATGTTGGCATTGAGGAAGAAAAAGGCAATGGAATGAATTCC
	TTGCTCGGCACTTTTTTGAAGAACCTGCAGGGTGATGGTTTTGAACAGATTTTTCAGTTT
	ATGCTTGGGTCTTATGTTGGCTGGCAGGGGAAGGAAGATGTACTGCGCGAACGATTGGAT
	TTGCTGGCCGAAAAAGTCAAAAGATTACCAAAGCCAAAATTTGCCGGAGAATGGAGTGGT
	CATCGTATGTTTCTCCATGGTCAGCTGAAAAGCTGGTCGTCGAATTTCTTCCGTCTTTTT
	AATGAGACGCGGGAACTTCTGGAAAGTATCAAGAGTGATATTCAACATGCCACCATGCTC
	ATTAGCTATGTGGAAGAGAAAGGAGGCTATCATCCACAGCTGTTGAGTCAGTATCGGAAG
	TTAATGGAACAATTACCGGCGTTGCGGACTAAGGTTTTGGATCCTGAGATTGAGATGACG
	CATATGTCCGAGGCTGTTCGAAGTTACATTATGATACACAAGTCTGTAGCGGGATTTCTG
	CCGGATTTACTCGAGTCTTTGGATCGAGATAAGGATAGGGAATTTTTGCTTTCCATCTTT
	CCTCGTATTCCAAAGATAGATAAGAAGACGAAAGAGATCGTTGCATGGGAGCTACCGGGC
	GAGCCAGAGGAAGGCTATTTGTTCACAGCAAACAACCTTTTCCGGAATTTTCTTGAGAAT
	CCGAAACATGTGCCACGATTTATGGCAGAGAGGATTCCCGAGGATTGGACGCGTTTGCGC
	TCGGCCCCTGTGTGGTTTGATGGGATGGTGAAGCAATGGCAGAAGGTGGTGAATCAGTTG
	GTTGAATCTCCAGGCGCCCTTTATCAGTTCAATGAAAGTTTTTTGCGTCAAAGACTGCAA
	GCAATGCTTACGGTCTATAAGCGGGATCTCCAGACTGAGAAGTTTCTGAAGCTGCTGGCT
	GATGTCTGTCGTCCACTCGTTGATTTTTTCGGACTTGGAGGAAATGATATTATCTTCAAG
	TCATGTCAGGATCCAAGAAAGCAATGGCAGACTGTTATTCCACTCAGTGTCCCAGCGGAT
	GTTTATACAGCATGTGAAGGCTTGGCTATTCGTCTCCGCGAAACTCTTGGATTCGAATGG
	AAAAATCTGAAAGGACACGAGCGGGAAGATTTTTTACGGCTGCATCAGTTGCTGGGAAAT
	CTGCTGTTCTGGATCAGGGATGCGAAACTTGTCGTGAAGCTGGAAGACTGGATGAACAAT
	CCTTGTGTTCAGGAGTATGTGGAAGCACGAAAAGCCATTGATCTTCCCTTGGAGATTTTC
	GGATTTGAGGTGCCGATTTTTCTCAATGGCTATCTCTTTTCGGAACTGCGCCAGCTGGAA
	TTGTTGCTGAGGCGTAAGTCGGTGATGACGTCTTACAGCGTCAAAACGACAGGCTCGCCA
	AATAGGCTCTTCCAGTTGGTTTACCTACCTCTAAACCCTTCAGATCCGGAAAAGAAAAAT
	TCCAACAACTTTCAGGAGCGCCTCGATACACCTACCGGTTTGTCGCGTCGTTTTCTGGAT
	CTTACGCTGGATGCATTTGCTGGCAAACTCTTGACGGATCCGGTAACTCAGGAACTGAAG
	ACGATGGCCGGTTTTTACGATCATCTCTTTGGCTTCAAGTTGCCGTGTAAACTGGCGGCG
	ATGAGTAACCATCCAGGATCCTCTTCCAAAATGGTGGTTCTGGCAAAACCAAAGAAGGGT
	GTTGCTAGTAACATCGGCTTTGAACCTATTCCCGATCCTGCTCATCCTGTGTTCCGGGTG
	AGAAGTTCCTGGCCGGAGTTGAAGTACCTGGAGGGGTTGTTGTATCTTCCCGAAGATACA
	CCACTGACCATTGAACTGGCGGAAACGTCGGTCAGTTGTCAGTCTGTGAGTTCAGTCGCT
	TTCGATTTGAAGAATCTGACGACTATCTTGGGTCGTGTTGGTGAATTCAGGGTGACGGCA
	GATCAACCTTTCAAGCTGACGCCCATTATTCCTGAGAAAGAGGAATCCTTCATCGGGAAG
	ACCTACCTCGGTCTTGATGCTGGAGAGCGATCTGGCGTTGGTTTCGCGATTGTGACGGTT
	GACGGCGATGGGTATGAGGTGCAGAGGTTGGGTGTGCATGAAGATACTCAGCTTATGGCG
	CTTCAGCAAGTCGCCAGCAAGTCTCTTAAGGAGCCGGTTTTCCAGCCACTCCGTAAGGGC
	ACATTTCGTCAGCAGGAGCGCATTCGCAAAAGCCTCCGCGGTTGCTACTGGAATTTCTAT
	CATGCATTGATGATCAAGTACCGAGCTAAAGTTGTGCATGAGGAATCGGTGGGTTCATCC
	GGTCTGGTGGGGCAGTGGCTGCGTGCATTTCAGAAGGATCTCAAAAAGGCTGATGTTCTG
	CCCAAGAAGGGTGGAAAAAATGGTGTAGACAAAAAAAAGAGAGAAAGCAGCGCTCAGGAT
	ACCTTATGGGGAGGAGCTTTCTCGAAGAAGGAAGAGCAGCAGATAGCCTTTGAGGTTCAG
	GCAGCTGGATCAAGCCAGTTTTGTCTGAAGTGTGGTTGGTGGTTTCAGTTGGGGATGCGG
	GAAGTAAATCGTGTGCAGGAGAGTGGCGTGGTGCTGGACTGGAACCGGTCCATTGTAACC
	TTCCTCATCGAATCCTCAGGAGAAAAGGTATATGGTTTCAGTCCTCAGCAACTGGAAAAA
	GGCTTTCGTCCTGACATCGAAACGTTCAAAAAAATGGTAAGGGATTTTATGAGACCCCCC
	ATGTTTGATCGCAAAGGTCGGCCGGCCGCGGCGTATGAAAGATTCGTACTGGGACGTCGT
	CACCGTCGTTATCGCTTTGATAAAGTTTTTGAAGAGAGATTTGGTCGCAGTGCTCTTTTC
	ATCTGCCCGCGGGTCGGGTGTGGGAATTTCGATCACTCCAGTGAGCAGTCAGCCGTTGTC
	CTTGCCCTTATTGGTTACATTGCTGATAAGGAAGGGATGAGTGGTAAGAAGCTTGTTTAT
	GTGAGGCTGGCTGAACTTATGGCTGAGTGGAAGCTGAAGAAACTGGAGAGATCAAGGGTG
	GAAGAACAGAGCTCGGCACAATAA

SEQ	ATGGCAGAAAGCAAGCAGATGCAATGCCGCAAGTGCGGCGCAAGCATGAAGTATGAAGTA
ID	ATTGGATTGGGCAAGAAGTCATGCAGATATATGTGCCCAGATTGCGGCAATCACACCAGC
NO:	GCGCGCAAGATTCAGAACAAGAAAAAGCGCGACAAAAAGTATGGATCCGCAAGCAAAGCG
38	CAGAGCCAGAGGATAGCTGTGGCTGGCGCGCTTTATCCAGACAAAAAAGTGCAGACCATA
	AAGACCTACAAATACCCAGCGGATCTTAATGGCGAAGTTCATGACAGCGGCGTCGCAGAG
	AAGATTGCGCAGGCGATTCAGGAAGATGAGATCGGCCTGCTTGGCCCGTCCAGCGAATAC
	GCTTGCTGGATTGCTTCACAAAAACAGAGCGAGCCGTATTCAGTTGTAGATTTTTGGTTT
	GACGCGGTGTGCGCAGGCGGAGTATTCGCGTATTCTGGCGCGCGCCTGCTTTCCACAGTC
	CTCCAGTTGAGTGGCGAGGAAAGCGTTTTGCGCGCTGCTTTAGCATCTAGCCCGTTTGTA
	GATGACATTAATTTGGCGCAAGCGGAAAAGTTCCTAGCCGTTAGCCGGCGCACAGGCCAA
	GATAAGCTAGGCAAGCGCATTGGAGAATGTTTTGCGGAAGGCCGGCTTGAAGCGCTTGGC
	ATCAAAGATCGCATGCGCGAATTCGTGCAAGCGATTGATGTGGCCCAAACCGCGGGCCAG
	CGGTTCGCGGCCAAGCTAAAGATATTCGGCATCAGTCAGATGCCTGAAGCCAAGCAATGG
	AACAATGATTCCGGGCTCACTGTATGTATTTTGCCGGATTATTATGTCCCGGAAGAAAAC
	CGCGCGGACCAGCTGGTTGTTTTGCTTCGGCGCTTACGCGAGATCGCGTATTGCATGGGA
	ATTGAGGATGAAGCAGGATTTGAGCATCTAGGCATTGACCCTGGTGCTCTTTCCAATTTT
	TCCAATGGCAATCCAAAGCGAGGATTTCTCGGCCGCCTGCTCAATAATGACATTATAGCG
	CTGGCAAACAACATGTCAGCCATGACGCCGTATTGGGAAGGCAGAAAAGGCGAGTTGATT
	GAGCGCCTTGCATGGCTTAAACATCGCGCTGAAGGATTGTATTTGAAAGAGCCACATTTC
	GGCAACTCCTGGGCAGACCACCGCAGCAGGATTTTCAGTCGCATTGCGGGCTGGCTTTCC
	GGATGCGCGGGCAAGCTCAAGATTGCCAAGGATCAGATTTCAGGCGTGCGTACGGATTTG
	TTTCTGCTCAAGCGCCTTCTGGATGCGGTACCGCAAAGCGCGCCGTCGCCGGACTTTATT
	GCTTCCATCAGCGCGCTGGATCGGTTTTTGGAAGCGGCAGAAAGCAGCCAGGATCCGGCA
	GAACAGGTACGCGCTTTGTACGCGTTTCATCTGAACGCGCCTGCGGTCCGATCCATCGCC
	AACAAGGCGGTACAGAGGTCTGATTCCCAGGAGTGGCTTATCAAGGAACTGGATGCTGTA
	GATCACCTTGAATTCAACAAAGCATTTCCGTTTTTTTCGGATACAGGAAAGAAAAAGAAG
	AAAGGAGCGAATAGCAACGGAGCGCCTTCTGAAGAAGAATACACGGAAACAGAATCCATT
	CAACAACCAGAAGATGCAGAGCAGGAAGTGAATGGTCAAGAAGGAAATGGCGCTTCAAAG
	AACCAGAAAAAGTTTCAGCGCATTCCTCGATTTTTCGGGGAAGGGTCAAGGAGTGAGTAT
	CGAATTTTAACAGAAGCGCCGCAATATTTTGACATGTTCTGCAATAATATGCGCGCGATC
	TTTATGCAGCTAGAGAGTCAGCCGCGCAAGGCGCCTCGTGATTTCAAATGCTTTCTGCAG
	AATCGTTTGCAGAAGCTTTACAAGCAAACCTTTCTCAATGCTCGCAGTAATAAATGCCGC
	GCGCTTCTGGAATCCGTCCTTATTTCATGGGGAGAATTTTATACTTATGGCGCGAATGAA
	AAGAAGTTTCGTCTGCGCCATGAAGCGAGCGAGCGCAGCTCGGATCCGGACTATGTGGTT
	CAGCAGGCATTGGAAATCGCGCGCCGGCTTTTCTTGTTCGGATTTGAGTGGCGCGATTGC
	TCTGCTGGAGAGCGCGTGGATTTGGTTGAAATCCACAAAAAAGCAATCTCATTTTTGCTT
	GCAATCACTCAGGCCGAGGTTTCAGTTGGTTCCTATAACTGGCTTGGGAATAGCACCGTG
	AGCCGGTATCTTTCGGTTGCTGGCACAGACACATTGTACGGCACTCAACTGGAGGAGTTT
	TTGAACGCCACAGTGCTTTCACAGATGCGTGGGCTGGCGATTCGGCTTTCATCTCAGGAG
	TTAAAAGACGGATTTGATGTTCAGTTGGAGAGTTCGTGCCAGGACAATCTCCAGCATCTG
	CTGGTGTATCGCGCTTCGCGCGACTTGGCTGCGTGCAAACGCGCTACATGCCCGGCTGAA
	TTGGATCCGAAAATTCTTGTTCTGCCGGTTGGTGCGTTTATCGCGAGCGTAATGAAAATG
	ATTGAGCGTGGCGATGAACCATTAGCAGGCGCGTATTTGCGTCATCGGCCGCATTCATTC
	GGCTGGCAGATACGGGTTCGTGGAGTGGCGGAAGTAGGCATGGATCAGGGCACAGCGCTA
	GCATTCCAGAAGCCGACTGAATCAGAGCCGTTTAAAATAAAGCCGTTTTCCGCTCAATAC
	GGCCCAGTACTTTGGCTTAATTCTTCATCCTATAGCCAGAGCCAGTATCTGGATGGATTT
	TTAAGCCAGCCAAAGAATTGGTCTATGCGGGTGCTACCTCAAGCCGGATCAGTGCGCGTG
	GAACAGCGCGTTGCTCTGATATGGAATTTGCAGGCAGGCAAGATGCGGCTGGAGCGCTCT
	GGAGCGCGCGCGTTTTTCATGCCAGTGCCATTCAGCTTCAGGCCGTCTGGTTCAGGAGAT
	GAAGCAGTATTGGCGCCGAATCGGTACTTGGGACTTTTTCCGCATTCCGGAGGAATAGAA
	TACGCGGTGGTGGATGTATTAGATTCCGCGGGTTTCAAAATTCTTGAGCGCGGTACGATT
	GCGGTAAATGGCTTTTCCCAGAAGCGCGGCGAACGCCAAGAGGAGGCACACAGAGAAAAA
	CAGAGACGCGGAATTTCTGATATAGGCCGCAAGAAGCCGGTGCAAGCTGAAGTTGACGCA
	GCCAATGAATTGCACCGCAAATACACCGATGTTGCCACTCGTTTAGGGTGCAGAATTGTG
	GTTCAGTGGGCGCCCCAGCCAAAGCCGGGCACAGCGCCGACCGCGCAAACAGTATACGCG
	CGCGCAGTGCGGACCGAAGCGCCGCGATCTGGAAATCAAGAGGATCATGCTCGTATGAAA
	TCCTCTTGGGGATATACCTGGGGCACCTATTGGGAGAAGCGCAAACCAGAGGATATTTTG
	GGCATCTCAACCCAAGTATACTGGACCGGCGGTATAGGCGAGTCATGTCCCGCAGTCGCG
	GTTGCGCTTTTGGGGCACATTAGGGCAACATCCACTCAAACTGAATGGGAAAAAGAGGAG
	GTTGTATTCGGTCGACTGAAGAAGTTCTTTCCAAGCTAG

SEQ	ATGGAAAAGAGAATAAACAAGATACGAAAGAAACTATCGGCCGATAATGCCACAAAGCCT
ID	GTGAGCAGGAGCGGCCCCATGAAAACACTCCTTGTCCGGGTCATGACGGACGACTTGAAA
NO:	AAAAGACTGGAGAAGCGTCGGAAAAAGCCGGAAGTTATGCCGCAGGTTATTTCAAATAAC
39	GCAGCAAACAATCTTAGAATGCTCCTTGATGACTATACAAAGATGAAGGAGGCGATACTA
	CAAGTTTACTGGCAGGAATTTAAGGACGACCATGTGGGCTTGATGTGCAAATTTGCCCAG
	CCTGCTTCCAAAAAAATTGACCAGAACAAACTAAAACCGGAAATGGATGAAAAAGGAAAT
	CTAACAACTGCCGGTTTTGCATGTTCTCAATGCGGTCAGCCGCTATTTGTTTATAAGCTT
	GAACAGGTGAGTGAAAAAGGCAAGGCTTATACAAATTACTTCGGCCGGTGTAATGTGGCC
	GAGCATGAGAAATTGATTCTTCTTGCTCAATTAAAACCTGAAAAAGACAGTGACGAAGCA
	GTGACATACTCCCTTGGCAAATTCGGCCAGAGGGCATTGGACTTTTATTCAATCCACGTA
	ACAAAAGAATCCACCCATCCAGTAAAGCCCCTGGCACAGATTGCGGGCAACCGCTATGCA
	AGCGGACCTGTTGGCAAGGCCCTTTCCGATGCCTGTATGGGCACTATAGCCAGTTTTCTT
	TCGAAATATCAAGACATCATCATAGAACATCAAAAGGTTGTGAAGGGTAATCAAAAGAGG
	TTAGAGAGTCTCAGGGAATTGGCAGGGAAAGAAAATCTTGAGTACCCATCGGTTACACTG
	CCGCCGCAGCCGCATACGAAAGAAGGGGTTGACGCTTATAACGAAGTTATTGCAAGGGTA
	CGTATGTGGGTTAATCTTAATCTGTGGCAAAAGCTGAAGCTCAGCCGTGATGACGCAAAA
	CCGCTACTGCGGCTAAAAGGATTCCCATCTTTCCCTGTTGTGGAGCGGCGTGAAAACGAA
	GTTGACTGGTGGAATACGATTAATGAAGTAAAAAAACTGATTGACGCTAAACGAGATATG
	GGACGGGTATTCTGGAGCGGCGTTACCGCAGAAAAGAGAAATACCATCCTTGAAGGATAC
	AACTATCTGCCAAATGAGAATGACCATAAAAAGAGAGAGGGCAGTTTGGAAAACCCTAAG
	AAGCCTGCCAAACGCCAGTTTGGAGACCTCTTGCTGTATCTTGAAAAGAAATATGCCGGA
	GACTGGGGAAAGGTCTTCGATGAGGCATGGGAGAGGATAGATAAGAAAATAGCCGGACTC
	ACAAGCCATATAGAGCGCGAAGAAGCAAGAAACGCGGAAGACGCTCAATCCAAAGCCGTA
	CTTACAGACTGGCTAAGGGCAAAGGCATCATTTGTTCTTGAAAGACTGAAGGAAATGGAT
	GAAAAGGAATTCTATGCGTGTGAAATCCAACTTCAAAAATGGTATGGCGATCTTCGAGGC
	AACCCGTTTGCCGTTGAAGCTGAGAATAGAGTTGTTGATATAAGCGGGTTTTCTATCGGA
	AGCGATGGCCATTCAATCCAATACAGAAATCTCCTTGCCTGGAAATATCTGGAGAACGGC
	AAGCGTGAATTCTATCTGTTAATGAATTATGGCAAGAAAGGGCGCATCAGATTTACAGAT
	GGAACAGATATTAAAAAGAGCGGCAAATGGCAGGGACTATTATATGGCGGTGGCAAGGCA
	AAGGTTATTGATCTGACTTTCGACCCCGATGATGAACAGTTGATAATCCTGCCGCTGGCC
	TTTGGCACAAGGCAAGGCCGCGAGTTTATCTGGAACGATTTGCTGAGTCTTGAAACAGGC
	CTGATAAAGCTCGCAAACGGAAGAGTTATCGAAAAAACAATCTATAACAAAAAAATAGGG
	CGGGATGAACCGGCTCTATTCGTTGCCTTAACATTTGAGCGCCGGGAAGTTGTTGATCCA
	TCAAATATAAAGCCTGTAAACCTTATAGGCGTTGACCGCGGCGAAAACATCCCGGCGGTT
	ATTGCATTGACAGACCCTGAAGGTTGTCCTTTACCGGAATTCAAGGATTCATCAGGGGGC
	CCAACAGACATCCTGCGAATAGGAGAAGGATATAAGGAAAAGCAGAGGGCTATTCAGGCA
	GCAAAGGAGGTAGAGCAAAGGCGGGCTGGCGGTTATTCACGGAAGTTTGCATCCAAGTCG
	AGGAACCTGGCGGACGACATGGTGAGAAATTCAGCGCGAGACCTTTTTTACCATGCCGTT
	ACCCACGATGCCGTCCTTGTCTTTGAAAACCTGAGCAGGGGTTTTGGAAGGCAGGGCAAA
	AGGACCTTCATGACGGAAAGACAATATACAAAGATGGAAGACTGGCTGACAGCGAAGCTC
	GCATACGAAGGTCTTACGTCAAAAACCTACCTTTCAAAGACGCTGGCGCAATATACGTCA
	AAAACATGCTCCAACTGCGGGTTTACTATAACGACTGCCGATTATGACGGGATGTTGGTA
	AGGCTTAAAAAGACTTCTGATGGATGGGCAACTACCCTCAACAACAAAGAATTAAAAGCC
	GAAGGCCAGATAACGTATTATAACCGGTATAAAAGGCAAACCGTGGAAAAAGAACTCTCC
	GCAGAGCTTGACAGGCTTTCAGAAGAGTCGGGCAATAATGATATTTCTAAGTGGACCAAG
	GGTCGCCGGGACGAGGCATTATTTTTGTTAAAGAAAAGATTCAGCCATCGGCCTGTTCAG
	GAACAGTTTGTTTGCCTCGATTGCGGCCATGAAGTCCACGCCGATGAACAGGCAGCCTTG
	AATATTGCAAGGTCATGGCTTTTTCTAAACTCAAATTCAACAGAATTCAAAAGTTATAAA
	TCGGGTAAACAGCCCTTCGTTGGTGCTTGGCAGGCCTTTTACAAAAGGAGGCTTAAAGAG
	GTATGGAAGCCCAACGCC

SEQ	ATGAAAAGGATAAATAAAATACGAAGGAGATTGGTAAAGGATAGCAACACGAAAAAAGCC
ID	GGCAAAACCGGCCCTATGAAAACCTTGCTCGTTCGGGTTATGACACCTGACCTGAGAGAA
NO:	AGGTTAGAGAATCTTCGCAAAAAGCCGGAAAACATTCCTCAGCCCATTTCAAATACTTCA
40	CGTGCAAATTTAAATAAACTCCTCACTGACTATACGGAAATGAAGAAAGCAATCCTGCAT
	GTTTATTGGGAAGAGTTCCAAAAAGACCCTGTCGGATTGATGAGCAGGGTTGCACAACCA
	GCGCCCAAGAATATTGATCAGAGAAAATTGATTCCGGTGAAGGACGGAAATGAGAGACTA
	ACAAGTTCTGGATTTGCCTGTTCTCAGTGCTGTCAACCCCTCTATGTTTATAAGCTTGAA
	CAAGTGAATGACAAGGGTAAGCCCCATACAAATTACTTTGGCCGTTGTAATGTCTCCGAG
	CATGAACGTTTGATATTGCTCTCGCCGCATAAACCGGAGGCAAATGACGAGCTAGTAACG
	TATTCGTTGGGGAAGTTCGGTCAAAGGGCATTGGACTTTTATTCAATCCACGTAACAAGA
	GAATCGAACCATCCTGTAAAGCCGCTAGAACAGATCGGTGGCAATAGCTGCGCAAGTGGT
	CCCGTTGGTAAGGCTTTATCTGATGCCTGTATGGGAGCAGTAGCCAGTTTCCTTACAAAG
	TACCAGGACATCATCCTCGAACACCAAAAGGTTATAAAAAAAAACGAAAAGAGATTGGCA
	AATCTAAAGGATATAGCAAGTGCAAACGGGCTTGCATTTCCTAAAATCACTCTTCCACCG
	CAACCGCATACAAAAGAAGGGATTGAAGCTTATAACAATGTTGTTGCTCAGATAGTGATC
	TGGGTAAACCTGAATCTTTGGCAGAAACTCAAAATTGGCAGGGATGAGGCAAAGCCCTTA
	CAGCGGCTTAAGGGTTTTCCGTCCTTCCCTCTTGTTGAACGCCAGGCGAATGAGGTTGAT
	TGGTGGGATATGGTCTGTAATGTCAAAAAGTTGATTAACGAAAAGAAAGAGGACGGGAAG
	GTCTTCTGGCAAAATCTTGCTGGATATAAAAGGCAGGAAGCCTTGCTTCCATATCTTTCG
	TCTGAAGAAGACCGTAAAAAAGGAAAAAAGTTTGCGCGTTATCAGTTTGGTGACCTTTTG
	CTTCACCTTGAAAAGAAACACGGTGAAGATTGGGGCAAAGTTTATGATGAGGCATGGGAA
	AGAATAGATAAAAAAGTTGAAGGTCTGAGTAAGCACATAAAGTTGGAGGAAGAAAGAAGG
	TCTGAAGATGCTCAATCAAAGGCTGCCCTCACTGATTGGCTCAGGGCAAAGGCCTCTTTT
	GTTATTGAAGGGCTCAAAGAAGCTGATAAGGATGAGTTTTGCAGGTGTGAGTTAAAGCTT
	CAAAAGTGGTATGGAGATTTGAGAGGAAAACCATTTGCTATAGAAGCAGAGAACAGCATT
	TTAGATATAAGCGGATTTTCTAAACAGTATAATTGTGCATTTATATGGCAGAAAGACGGC
	GTAAAGAAGTTAAATCTTTATTTAATAATAAATTACTTCAAAGGTGGTAAGCTACGCTTC
	AAAAAAATCAAGCCAGAAGCTTTTGAAGCAAATAGGTTTTATACAGTAATTAATAAAAAA
	AGCGGTGAGATTGTGCCTATGGAGGTCAACTTCAATTTTGATGACCCGAATTTGATAATT
	CTGCCTTTGGCCTTTGGAAAAAGGCAGGGGAGGGAGTTTATCTGGAACGACCTATTGAGC
	CTTGAGACGGGTTCATTGAAACTCGCCAATGGCAGGGTTATTGAAAAAACGCTCTATAAC
	AGAAGGACGAGACAGGATGAACCAGCACTTTTTGTTGCCCTGACATTTGAAAGAAGAGAG
	GTGCTTGACTCATCGAATATAAAACCGATGAATCTGATAGGAATAGACCGGGGAGAAAAT
	ATCCCGGCAGTCATAGCATTAACAGACCCGGAAGGATGCCCCTTGTCAAGATTCAAAGAT
	TCATTGGGCAATCCAACGCATATTTTGCGAATAGGAGAAAGTTATAAGGAAAAACAACGG
	ACTATTCAGGCTGCTAAAGAAGTTGAACAAAGGCGGGCAGGCGGATATTCGAGAAAATAT
	GCATCAAAGGCGAAGAATCTGGCGGACGATATGGTAAGAAATACAGCTCGTGACCTCTTA
	TATTATGCTGTTACTCAAGATGCAATGCTCATTTTTGAAAATCTTTCCCGCGGTTTTGGT
	AGACAAGGCAAGAGGACTTTTATGGCGGAAAGGCAGTACACGAGGATGGAAGACTGGCTG
	ACTGCAAAGCTTGCCTATGAAGGTCTGCCATCAAAAACCTATCTTTCAAAGACTCTGGCA
	CAGTATACCTCAAAGACATGTTCTAATTGTGGTTTTACAATCACAAGTGCAGATTATGAC
	AGGGTGCTCGAAAAGCTCAAGAAGACGGCTACTGGATGGATGACTACAATCAATGGAAAA
	GAGTTAAAAGTTGAAGGACAGATAACATACTATAACCGGTATAAAAGGCAGAATGTGGTA
	AAAGACCTCTCTGTAGAGCTGGATAGACTTTCGGAAGAGTCGGTAAATAATGATATTTCT
	AGTTGGACAAAAGGCCGCAGTGGTGAAGCTTTATCTCTGCTAAAAAAGAGATTTAGTCAC
	AGGCCGGTGCAGGAAAAGTTTGTTTGCCTGAACTGTGGTTTTGAAACCCATGCAGACGAA
	CAAGCAGCACTGAATATTGCAAGGTCGTGGCTCTTTCTCCGTTCTCAAGAATATAAGAAG
	TATCAAACCAATAAAACGACCGGAAATACTGACAAAAGGGCATTTGTTGAAACATGGCAA
	TCCTTTTACAGAAAGAAGCTCAAAGAAGTATGGAAACCA

SEQ	ATGGGTAAAATGTATTACCTTGGTTTAGACATTGGCACGAATTCCGTGGGCTACGCGGTG
ID	ACCGACCCCTCATACCACCTGCTGAAGTTTAAGGGGGAACCAATGTGGGGTGCGCACGTA
NO:	TTTGCCGCCGGTAATCAGAGCGCGGAACGACGCTCGTTCCGCACATCGCGTCGTCGTTTG
41	GACCGACGCCAACAGCGCGTTAAACTGGTACAGGAGATTTTTGCCCCGGTGATTAGTCCG
	ATCGACCCACGCTTCTTCATTCGTCTGCATGAATCCGCCCTGTGGCGCGATGACGTCGCG
	GAGACGGATAAACATATCTTTTTCAATGATCCTACCTATACCGATAAGGAATATTATAGC
	GATTACCCGACTATCCATCACCTGATCGTTGATCTGATGGAAAGCTCTGAGAAACACGAT
	CCGCGGCTGGTGTACCTTGCAGTGGCGTGGTTAGTGGCACACCGTGGTCATTTTCTGAAC
	GAGGTGGACAAGGATAATATTGGAGATGTGTTGTCGTTCGACGCATTTTATCCGGAGTTT
	CTCGCGTTCCTGTCGGACAACGGTGTATCACCGTGGGTGTGCGAAAGCAAAGCGCTGCAG
	GCGACCTTGCTGAGCCGTAACTCAGTGAACGACAAATATAAAGCCCTTAAGTCTCTGATC
	TTCGGATCCCAGAAACCTGAAGATAACTTCGATGCCAATATTTCGGAAGATGGACTCATT
	CAACTGCTGGCCGGCAAAAAGGTAAAAGTTAACAAACTGTTCCCTCAGGAATCGAACGAT
	GCATCCTTCACATTGAATGATAAAGAAGACGCGATAGAAGAAATCCTGGGTACGCTTACA
	CCAGATGAATGTGAATGGATTGCGCATATACGCCGCCTTTTTGACTGGGCTATCATGAAA
	CATGCTCTGAAAGATGGCAGGACTATTAGCGAGTCAAAAGTCAAACTGTATGAGCAGCAC
	CATCACGATCTGACCCAACTTAAATACTTCGTGAAAACCTACCTTGCAAAAGAATACGAC
	GATATTTTCCGCAACGTGGATAGCGAAACAACGAAAAACTATGTAGCGTATTCCTATCAT
	GTGAAAGAGGTGAAAGGCACTCTGCCTAAAAATAAGGCAACGCAAGAAGAGTTTTGTAAG
	TATGTCCTGGGCAAGGTTAAAAACATTGAATGCTCTGAAGCAGACAAGGTTGACTTTGAT
	GAGATGATTCAGCGTCTTACCGACAACTCTTTTATGCCTAAGCAGGTTTCGGGCGAAAAC
	CGCGTTATTCCTTATCAGTTATATTATTATGAACTGAAGACAATTCTGAATAAAGCAGCC
	TCGTACCTGCCTTTCCTGACGCAGTGTGGAAAAGATGCAATTTCGAACCAGGACAAACTA
	CTGTCGATCATGACGTTCCGTATTCCTTACTTCGTCGGACCCTTGCGAAAAGATAATTCG
	GAACATGCATGGCTCGAACGAAAGGCCGGTAAGATTTATCCGTGGAACTTTAACGACAAA
	GTGGACTTGGATAAATCAGAAGAAGCGTTCATTCGCCGAATGACCAATACCTGTACCTAT
	TATCCCGGCGAAGATGTTTTACCGTTGGATTCGCTGATCTATGAGAAATTTATGATTTTA
	AATGAAATCAATAATATTCGTATTGACGGCTACCCGATTAGTGTTGACGTTAAACAGCAG
	GTTTTTGGCTTGTTCGAAAAAAAACGACGCGTAACCGTGAAAGATATTCAGAACCTGCTG
	CTGTCTCTCGGAGCTCTGGACAAACACGGGAAGCTGACAGGCATCGATACCACTATCCAC
	TCAAACTATAATACGTATCACCATTTTAAATCTCTCATGGAACGCGGCGTCCTGACCCGG
	GATGACGTGGAACGCATCGTTGAAAGGATGACCTACAGCGACGATACTAAGCGTGTGCGT
	CTGTGGCTGAATAACAACTATGGTACTTTAACCGCCGACGATGTGAAACACATTTCGCGT
	CTGCGCAAACACGATTTTGGCCGTTTATCCAAAATGTTCTTAACAGGTCTGAAGGGTGTC
	CATAAGGAGACCGGTGAACGTGCCTCCATACTGGATTTCATGTGGAACACGAACGATAAC
	CTGATGCAGCTCCTTTCCGAATGCTACACGTTCAGTGATGAAATCACAAAGCTGCAAGAG
	GCGTATTATGCAAAAGCCCAGTTGTCTTTAAACGATTTTTTAGACTCGATGTACATCTCT
	AACGCGGTGAAACGTCCGATTTACAGAACTCTGGCAGTGGTGAACGATATTCGAAAAGCA
	TGTGGGACGGCCCCTAAACGCATTTTCATCGAAATGGCTCGTGATGGTGAATCAAAAAAA
	AAGAGAAGTGTTACACGTCGCGAGCAGATCAAAAACCTGTACCGCTCGATTCGTAAAGAT
	TTCCAGCAGGAAGTTGATTTTCTGGAAAAGATCCTGGAAAATAAATCTGATGGTCAACTT
	CAGTCAGATGCTTTGTATCTTTACTTTGCACAATTAGGGCGCGATATGTACACGGGCGAT
	CCAATAAAGCTGGAGCACATCAAAGATCAGAGTTTCTATAACATAGACCATATTTACCCG
	CAGTCTATGGTGAAAGACGATTCCCTAGATAACAAAGTGCTGGTGCAAAGCGAAATTAAC
	GGCGAGAAAAGCTCGCGATACCCTTTGGACGCCGCGATCCGCAATAAAATGAAGCCCCTT
	TGGGACGCTTACTATAATCATGGCCTGATCTCCTTAAAGAAATACCAGCGTCTAACGCGC
	TCGACCCCGTTTACCGATGATGAAAAATGGGACTTTATTAATCGCCAGTTAGTGGAAACC
	CGTCAATCTACCAAAGCGCTGGCCATTTTGTTGAAGCGTAAGTTTCCAGACACCGAAATT
	GTGTATTCGAAGGCGGGGTTATCGTCCGACTTCAGACATGAATTCGGCCTTGTAAAAAGT
	CGCAATATTAATGATTTGCACCACGCTAAAGACGCATTCTTGGCTATCGTTACCGGCAAT
	GTGTACCATGAAAGATTCAATCGCAGATGGTTTATGGTGAACCAGCCGTACTCAGTTAAA
	ACTAAAACTCTTTTTACCCACAGCATAAAGAATGGCAACTTCGTTGCCTGGAACGGCGAA
	GAAGATCTCGGTCGTATTGTAAAAATGCTGAAGCAAAACAAAAATACCATTCACTTCACG
	CGCTTCTCCTTCGATCGCAAAGAAGGATTATTTGATATCCAACCTCTGAAAGCCAGCACC
	GGCTTAGTCCCACGAAAAGCCGGTCTGGATGTCGTTAAATACGGCGGATATGACAAATCT
	ACCGCGGCCTATTACCTGCTGGTGAGGTTCACGCTCGAGGACAAGAAAACCCAGCACAAG
	CTGATGATGATTCCTGTAGAAGGCCTGTACAAGGCTCGCATTGATCATGACAAGGAATTT
	CTTACCGATTATGCGCAAACGACTATAAGCGAAATCCTACAGAAAGATAAACAGAAAGTG
	ATCAATATTATGTTTCCAATGGGTACGAGGCATATAAAACTCAATTCAATGATTAGTATC
	GATGGCTTCTATCTTAGTATCGGCGGAAAGTCCTCTAAAGGTAAGTCAGTTCTATGTCAC
	GCAATGGTTCCACTGATCGTCCCTCACAAAATCGAATGTTACATTAAAGCAATGGAAAGC
	TTCGCCCGGAAGTTTAAAGAAAACAACAAGCTGCGCATCGTAGAAAAATTCGATAAAATC
	ACCGTTGAAGACAACCTGAATCTCTACGAGCTCTTTCTCCAAAAACTGCAGCATAATCCC
	TATAATAAGTTTTTTTCGACACAGTTTGACGTACTGACGAACGGCCGTTCTACTTTCACA
	AAACTGTCGCCGGAGGAACAGGTACAGACGCTCTTGAACATTTTAAGTATCTTTAAAACA
	TGCCGCAGTTCGGGTTGCGACCTGAAATCCATCAACGGCAGTGCCCAGGCAGCGCGCATC
	ATGATTAGCGCTGACTTAACTGGACTGTCGAAAAAATATTCAGATATTAGGTTGGTTGAA
	CAGTCAGCTTCTGGTTTGTTCGTATCCAAAAGTCAGAACTTACTGGAGTATCTCTAA

SEQ	ATGTCATCGCTCACGAAATTCACTAACAAATACTCTAAACAGCTCACCATTAAGAATGAA
ID	CTCATCCCAGTTGGCAAAACACTGGAGAACATCAAAGAGAATGGTCTGATAGATGGCGAC
NO:	GAACAGCTGAATGAGAATTATCAGAAGGCGAAAATTATTGTGGATGATTTTCTGCGGGAC
42	TTCATTAATAAAGCACTGAATAATACGCAGATCGGGAACTGGCGCGAACTGGCGGATGCC
	CTTAATAAAGAGGATGAAGATAACATCGAGAAATTGCAGGATAAAATTCGGGGAATCATT
	GTATCCAAATTTGAAACGTTTGATCTGTTTAGCAGCTATTCTATTAAGAAAGATGAAAAG
	ATTATTGACGACGACAATGATGTTGAAGAAGAGGAACTGGATCTGGGCAAGAAGACCAGC
	TCATTTAAATACATATTTAAAAAAAACCTGTTTAAGTTAGTGTTGCCATCCTACCTGAAA
	ACCACAAACCAGGACAAGCTGAAGATTATTAGCTCGTTTGATAATTTTTCAACGTACTTC
	CGCGGGTTCTTTGAAAACCGGAAAAACATTTTTACCAAGAAACCGATCTCCACAAGTATT
	GCGTATCGCATTGTTCATGATAACTTCCCGAAATTCCTTGATAACATTCGTTGTTTTAAT
	GTGTGGCAGACGGAATGCCCGCAACTAATCGTGAAAGCAGATAACTATCTGAAAAGCAAA
	AATGTTATAGCGAAAGATAAAAGTTTGGCAAACTATTTTACCGTGGGCGCGTATGACTAT
	TTCCTGTCTCAGAATGGTATAGATTTTTACAACAATATTATAGGTGGACTGCCAGCGTTC
	GCCGGCCATGAGAAAATCCAAGGTCTCAATGAATTCATCAATCAAGAGTGCCAAAAAGAC
	AGCGAGCTGAAAAGTAAGCTGAAAAACCGTCACGCGTTCAAAATGGCGGTACTGTTCAAA
	CAGATACTCAGCGATCGTGAAAAAAGTTTTGTAATTGATGAGTTCGAGTCGGATGCTCAA
	GTTATTGACGCCGTTAAAAACTTTTACGCCGAACAGTGCAAAGATAACAATGTTATTTTT
	AACTTATTAAATCTTATCAAGAATATCGCTTTCTTAAGTGATGACGAACTGGACGGCATA
	TTCATTGAAGGGAAATACCTGTCGAGCGTTAGTCAAAAACTCTATAGCGATTGGTCAAAA
	TTACGTAACGACATTGAGGATTCGGCTAACTCTAAACAAGGCAATAAAGAGCTGGCCAAG
	AAGATCAAAACCAACAAAGGGGATGTAGAAAAAGCGATCTCGAAATATGAGTTCTCGCTG
	TCGGAACTGAACTCGATTGTACATGATAACACCAAGTTTTCTGACCTCCTTAGTTGTACA
	CTGCATAAGGTGGCTTCTGAGAAACTGGTGAAGGTCAATGAAGGCGACTGGCCGAAACAT
	CTCAAGAATAATGAAGAGAAACAAAAAATCAAAGAGCCGCTTGATGCTCTGCTGGAGATC
	TATAATACACTTCTGATTTTTAACTGCAAAAGCTTCAATAAAAACGGCAACTTCTATGTC
	GACTATGATCGTTGCATCAATGAACTGAGTTCGGTCGTGTATCTGTATAATAAAACACGT
	AACTATTGCACTAAAAAACCCTATAACACGGACAAGTTCAAACTCAATTTTAACAGTCCG
	CAGCTCGGTGAAGGCTTTTCCAAGTCGAAAGAAAATGACTGTCTGACTCTTTTGTTTAAA
	AAAGACGACAACTATTATGTAGGCATTATCCGCAAAGGTGCAAAAATCAATTTTGATGAT
	ACACAAGCAATCGCCGATAACACCGACAATTGCATCTTTAAAATGAATTATTTCCTACTT
	AAAGACGCAAAAAAATTTATCCCGAAATGTAGCATTCAGCTGAAAGAAGTCAAGGCCCAT
	TTTAAGAAATCTGAAGATGATTACATTTTGTCTGATAAAGAGAAATTTGCTAGCCCGCTG
	GTCATTAAAAAGAGCACATTTTTGCTGGCAACTGCACATGTGAAAGGGAAAAAAGGCAAT
	ATCAAGAAATTTCAGAAAGAATATTCGAAAGAAAACCCCACTGAGTATCGCAATTCTTTA
	AACGAATGGATTGCTTTTTGTAAAGAGTTCTTAAAAACTTATAAAGCGGCTACCATTTTT
	GATATAACCACATTGAAAAAGGCAGAGGAATATGCTGATATTGTAGAATTCTACAAGGAT
	GTCGATAATCTGTGCTACAAACTGGAGTTCTGCCCGATTAAAACCTCGTTTATAGAAAAC
	CTGATAGATAACGGCGACCTGTATCTGTTTCGCATCAATAACAAAGACTTCAGCAGTAAA
	TCGACCGGCACCAAGAACCTTCATACGTTATATTTACAAGCTATATTCGATGAACGTAAT
	CTGAACAATCCGACAATTATGCTGAATGGGGGAGCAGAACTGTTCTATCGTAAAGAAAGT
	ATTGAGCAGAAAAACCGTATCACACACAAAGCCGGTTCAATTCTCGTGAATAAGGTGTGT
	AAAGACGGTACAAGCCTGGATGATAAGATACGTAATGAAATTTATCAATATGAGAATAAA
	TTTATTGATACCCTGTCTGATGAAGCTAAAAAGGTGTTACCGAATGTCATTAAAAAGGAA
	GCTACCCATGACATTACAAAAGATAAACGTTTCACTAGTGACAAATTCTTCTTTCACTGC
	CCCCTGACAATTAATTATAAGGAAGGCGATACCAAGCAGTTCAATAACGAAGTGCTGAGT
	TTTCTGCGTGGAAATCCTGACATCAACATTATCGGCATTGACCGCGGAGAGCGTAATTTA
	ATCTATGTAACGGTTATAAACCAGAAAGGCGAGATTCTGGATTCGGTTTCATTCAATACC
	GTGACCAACAAGAGTTCAAAAATCGAGCAGACAGTCGATTATGAAGAGAAATTGGCAGTC
	CGCGAGAAAGAGAGGATTGAAGCAAAACGTTCCTGGGACTCTATCTCAAAAATTGCGACA
	CTAAAGGAAGGTTATCTGAGCGCAATAGTTCACGAGATCTGTCTGTTAATGATTAAACAC
	AACGCGATCGTTGTCTTAGAGAATCTTAATGCAGGCTTTAAGCGTATTCGTGGCGGTTTA
	TCAGAAAAAAGTGTTTATCAAAAATTCGAAAAAATGTTGATTAACAAACTGAACTATTTT
	GTCAGCAAGAAGGAATCCGACTGGAATAAACCGTCTGGTCTGCTGAATGGACTGCAGCTT
	TCGGATCAGTTTGAAAGCTTCGAAAAACTGGGTATTCAGTCTGGTTTTATTTTTTACGTG
	CCGGCTGCATATACCTCAAAGATTGATCCGACCACGGGCTTCGCCAATGTTCTGAATCTG
	TCGAAGGTACGCAATGTTGATGCGATCAAAAGCTTTTTTTCTAACTTCAACGAAATTAGT
	TATAGCAAGAAAGAAGCCCTTTTCAAATTCTCATTCGATCTGGATTCACTGAGTAAGAAA
	GGCTTTAGTAGCTTTGTGAAATTTAGTAAGAGTAAATGGAACGTCTACACCTTTGGAGAA
	CGTATCATAAAGCCAAAGAATAAGCAAGGTTATCGGGAGGACAAAAGAATCAACTTGACC
	TTCGAGATGAAGAAGTTACTTAACGAGTATAAGGTTTCTTTTGATCTTGAAAATAACTTG
	ATTCCGAATCTCACGAGTGCCAACCTGAAGGATACTTTTTGGAAAGAGCTATTCTTTATC
	TTCAAGACTACGCTGCAGCTCCGTAACAGCGTTACTAACGGTAAAGAAGATGTGCTCATC
	TCTCCGGTCAAAAATGCGAAGGGTGAATTCTTCGTTTCGGGAACGCATAACAAGACTCTT
	CCGCAAGATTGCGATGCGAACGGTGCATACCATATTGCGTTGAAAGGTCTGATGATACTC
	GAACGTAACAACCTTGTACGTGAGGAGAAAGATACGAAAAAGATTATGGCGATTTCAAAC
	GTGGATTGGTTCGAGTACGTGCAGAAACGTAGAGGCGTTCTGTAA

SEQ	ATGAACAACTACGACGAATTCACCAAACTGTACCCGATCCAGAAAACCATCCGTTTCGAA
ID	CTGAAACCGCAGGGTCGTACCATGGAACACCTGGAAACCTTCAACTTCTTCGAAGAAGAC
NO:	CGTGACCGTGCGGAAAAATACAAAATCCTGAAAGAAGCGATCGACGAATACCACAAAAAA
43	TTCATCGACGAACACCTGACCAACATGTCTCTGGACTGGAACTCTCTGAAACAGATCTCT
	GAAAAATACTACAAATCTCGTGAAGAAAAAGACAAAAAAGTTTTCCTGTCTGAACAGAAA
	CGTATGCGTCAGGAAATCGTTTCTGAATTCAAAAAAGACGACCGTTTCAAAGACCTGTTC
	TCTAAAAAACTGTTCTCTGAACTGCTGAAAGAAGAAATCTACAAAAAAGGTAACCACCAG
	GAAATCGACGCGCTGAAATCTTTCGACAAATTCTCTGGTTACTTCATCGGTCTGCACGAA
	AACCGTAAAAACATGTACTCTGACGGTGACGAAATCACCGCGATCTCTAACCGTATCGTT
	AACGAAAACTTCCCGAAATTCCTGGACAACCTGCAGAAATACCAGGAAGCGCGTAAAAAA
	TACCCGGAATGGATCATCAAAGCGGAATCTGCGCTGGTTGCGCACAACATCAAAATGGAC
	GAAGTTTTCTCTCTGGAATACTTCAACAAAGTTCTGAACCAGGAAGGTATCCAGCGTTAC
	AACCTGGCGCTGGGTGGTTACGTTACCAAATCTGGTGAAAAAATGATGGGTCTGAACGAC
	GCGCTGAACCTGGCGCACCAGTCTGAAAAATCTTCTAAAGGTCGTATCCACATGACCCCG
	CTGTTCAAACAGATCCTGTCTGAAAAAGAATCTTTCTCTTACATCCCGGACGTTTTCACC
	GAAGACTCTCAGCTGCTGCCGTCTATCGGTGGTTTCTTCGCGCAGATCGAAAACGACAAA
	GACGGTAACATCTTCGACCGTGCGCTGGAACTGATCTCTTCTTACGCGGAATACGACACC
	GAACGTATCTACATCCGTCAGGCGGACATCAACCGTGTTTCTAACGTTATCTTCGGTGAA
	TGGGGTACCCTGGGTGGTCTGATGCGTGAATACAAAGCGGACTCTATCAACGACATCAAC
	CTGGAACGTACCTGCAAAAAAGTTGACAAATGGCTGGACTCTAAAGAATTCGCGCTGTCT
	GACGTTCTGGAAGCGATCAAACGTACCGGTAACAACGACGCGTTCAACGAATACATCTCT
	AAAATGCGTACCGCGCGTGAAAAAATCGACGCGGCGCGTAAAGAAATGAAATTCATCTCT
	GAAAAAATCTCTGGTGACGAAGAATCTATCCACATCATCAAAACCCTGCTGGACTCTGTT
	CAGCAGTTCCTGCACTTCTTCAACCTGTTCAAAGCGCGTCAGGACATCCCGCTGGACGGT
	GCGTTCTACGCGGAATTCGACGAAGTTCACTCTAAACTGTTCGCGATCGTTCCGCTGTAC
	AACAAAGTTCGTAACTACCTGACCAAAAACAACCTGAACACCAAAAAAATCAAACTGAAC
	TTCAAAAACCCGACCCTGGCGAACGGTTGGGACCAGAACAAAGTTTACGACTACGCGTCT
	CTGATCTTCCTGCGTGACGGTAACTACTACCTGGGTATCATCAACCCGAAACGTAAAAAA
	AACATCAAATTCGAACAGGGTTCTGGTAACGGTCCGTTCTACCGTAAAATGGTTTACAAA
	CAGATCCCGGGTCCGAACAAAAACCTGCCGCGTGTTTTCCTGACCTCTACCAAAGGTAAA
	AAAGAATACAAACCGTCTAAAGAAATCATCGAAGGTTACGAAGCGGACAAACACATCCGT
	GGTGACAAATTCGACCTGGACTTCTGCCACAAACTGATCGACTTCTTCAAAGAATCTATC
	GAAAAACACAAAGACTGGTCTAAATTCAACTTCTACTTCTCTCCGACCGAATCTTACGGT
	GACATCTCTGAATTCTACCTGGACGTTGAAAAACAGGGTTACCGTATGCACTTCGAAAAC
	ATCTCTGCGGAAACCATCGACGAATACGTTGAAAAAGGTGACCTGTTCCTGTTCCAGATC
	TACAACAAAGACTTCGTTAAAGCGGCGACCGGTAAAAAAGACATGCACACCATCTACTGG
	AACGCGGCGTTCTCTCCGGAAAACCTGCAGGACGTTGTTGTTAAACTGAACGGTGAAGCG
	GAACTGTTCTACCGTGACAAATCTGACATCAAAGAAATCGTTCACCGTGAAGGTGAAATC
	CTGGTTAACCGTACCTACAACGGTCGTACCCCGGTTCCGGACAAAATCCACAAAAAACTG
	ACCGACTACCACAACGGTCGTACCAAAGACCTGGGTGAAGCGAAAGAATACCTGGACAAA
	GTTCGTTACTTCAAAGCGCACTACGACATCACCAAAGACCGTCGTTACCTGAACGACAAA
	ATCTACTTCCACGTTCCGCTGACCCTGAACTTCAAAGCGAACGGTAAAAAAAACCTGAAC
	AAAATGGTTATCGAAAAATTCCTGTCTGACGAAAAAGCGCACATCATCGGTATCGACCGT
	GGTGAACGTAACCTGCTGTACTACTCTATCATCGACCGTTCTGGTAAAATCATCGACCAG
	CAGTCTCTGAACGTTATCGACGGTTTCGACTACCGTGAAAAACTGAACCAGCGTGAAATC
	GAAATGAAAGACGCGCGTCAGTCTTGGAACGCGATCGGTAAAATCAAAGACCTGAAAGAA
	GGTTACCTGTCTAAAGCGGTTCACGAAATCACCAAAATGGCGATCCAGTACAACGCGATC
	GTTGTTATGGAAGAACTGAACTACGGTTTCAAACGTGGTCGTTTCAAAGTTGAAAAACAG
	ATCTACCAGAAATTCGAAAACATGCTGATCGACAAAATGAACTACCTGGTTTTCAAAGAC
	GCGCCGGACGAATCTCCGGGTGGTGTTCTGAACGCGTACCAGCTGACCAACCCGCTGGAA
	TCTTTCGCGAAACTGGGTAAACAGACCGGTATCCTGTTCTACGTTCCGGCGGCGTACACC
	TCTAAAATCGACCCGACCACCGGTTTCGTTAACCTGTTCAACACCTCTTCTAAAACCAAC
	GCGCAGGAACGTAAAGAATTCCTGCAGAAATTCGAATCTATCTCTTACTCTGCGAAAGAC
	GGTGGTATCTTCGCGTTCGCGTTCGACTACCGTAAATTCGGTACCTCTAAAACCGACCAC
	AAAAACGTTTGGACCGCGTACACCAACGGTGAACGTATGCGTTACATCAAAGAAAAAAAA
	CGTAACGAACTGTTCGACCCGTCTAAAGAAATCAAAGAAGCGCTGACCTCTTCTGGTATC
	AAATACGACGGTGGTCAGAACATCCTGCCGGACATCCTGCGTTCTAACAACAACGGTCTG
	ATCTACACCATGTACTCTTCTTTCATCGCGGCGATCCAGATGCGTGTTTACGACGGTAAA
	GAAGACTACATCATCTCTCCGATCAAAAACTCTAAAGGTGAATTCTTCCGTACCGACCCG
	AAACGTCGTGAACTGCCGATCGACGCGGACGCGAACGGTGCGTACAACATCGCGCTGCGT
	GGTGAACTGACCATGCGTGCGATCGCGGAAAAATTCGACCCGGACTCTGAAAAAATGGCG
	AAACTGGAACTGAAACACAAAGACTGGTTCGAATTCATGCAGACCCGTGGTGACTAA

SEQ	ATGACTAAAACATTTGATTCAGAGTTTTTTAATTTGTACTCGCTGCAAAAAACGGTACGC
ID	TTTGAGTTAAAACCCGTGGGAGAAACCGCGTCATTTGTGGAAGACTTTAAAAACGAGGGC
NO:	TTGAAACGTGTTGTGAGCGAAGATGAAAGGCGAGCCGTCGATTACCAGAAAGTTAAGGAA
44	ATAATTGACGATTACCATCGGGATTTCATTGAAGAAAGTTTAAATTATTTTCCGGAACAG
	GTGAGTAAAGATGCTCTTGAGCAGGCGTTTCATCTTTATCAGAAACTGAAGGCAGCAAAA
	GTTGAGGAAAGGGAAAAAGCGCTGAAAGAATGGGAAGCGCTGCAGAAAAAGCTACGTGAA
	AAAGTGGTGAAATGCTTCTCGGACTCGAATAAAGCCCGCTTCTCAAGGATTGATAAAAAG
	GAACTGATTAAGGAAGACCTGATAAATTGGTTGGTCGCCCAGAATCGCGAGGATGATATC
	CCTACGGTCGAAACGTTTAACAACTTCACCACATATTTTACCGGCTTCCATGAGAATCGT
	AAAAATATTTACTCCAAAGATGATCACGCCACCGCTATTAGCTTTCGCCTTATTCATGAA
	AATCTTCCAAAGTTTTTTGACAACGTGATTAGCTTCAATAAGTTGAAAGAGGGTTTCCCT
	GAATTAAAATTTGATAAAGTGAAAGAGGATTTAGAAGTAGATTATGATCTGAAGCATGCG
	TTTGAAATAGAATATTTCGTTAACTTCGTGACCCAAGCGGGCATAGATCAGTATAATTAT
	CTGTTAGGAGGGAAAACCCTGGAGGACGGGACGAAAAAACAAGGGATGAATGAGCAAATT
	AATCTGTTCAAACAACAGCAAACGCGAGATAAAGCGCGTCAGATTCCCAAACTGATCCCC
	CTGTTCAAACAGATTCTTAGCGAAAGGACTGAAAGCCAGTCCTTTATTCCTAAACAATTT
	GAAAGTGATCAGGAGTTGTTCGATTCACTGCAGAAGTTACATAATAACTGCCAGGATAAA
	TTCACCGTGCTGCAACAAGCCATTCTCGGTCTGGCAGAGGCGGATCTTAAGAAGGTCTTC
	ATCAAAACCTCTGATTTAAATGCCTTATCTAACACCATTTTCGGGAATTACAGCGTCTTT
	TCCGATGCACTGAACCTGTATAAAGAAAGCCTGAAAACGAAAAAAGCGCAGGAGGCTTTT
	GAGAAACTACCGGCCCATTCTATTCACGACCTCATTCAATACTTGGAACAGTTCAATTCC
	AGCCTGGACGCGGAAAAACAACAGAGCACCGACACCGTCCTGAACTACTTCATCAAGACC
	GATGAATTATATTCTCGCTTCATTAAATCCACTAGCGAGGCTTTCACTCAGGTGCAGCCT
	TTGTTCGAACTGGAAGCCCTGTCATCTAAGCGCCGCCCACCGGAATCGGAAGATGAAGGG
	GCAAAAGGGCAGGAAGGCTTCGAGCAGATCAAGCGTATTAAAGCTTACCTGGATACGCTT
	ATGGAAGCGGTACACTTTGCAAAGCCGTTGTATCTTGTTAAGGGTCGTAAAATGATCGAA
	GGGCTCGATAAAGACCAGTCCTTTTATGAAGCGTTTGAAATGGCGTACCAAGAACTTGAA
	TCGTTAATCATTCCTATCTATAACAAAGCGCGGAGCTATCTGTCGCGGAAACCTTTCAAG
	GCCGATAAATTCAAGATTAATTTTGACAACAACACGCTACTGAGCGGATGGGATGCGAAC
	AAGGAAACTGCTAACGCGTCCATTCTGTTTAAGAAAGACGGGTTATATTACCTTGGAATT
	ATGCCGAAAGGTAAGACCTTTCTCTTTGACTACTTTGTATCGAGCGAGGATTCAGAGAAA
	CTGAAACAGCGTCGCCAGAAGACCGCCGAAGAAGCTCTGGCGCAGGATGGTGAAAGTTAC
	TTCGAAAAAATTCGTTATAAACTGTTACCAGGGGCTTCAAAGATGTTACCGAAAGTCTTT
	TTTAGCAACAAAAATATTGGCTTTTACAACCCGTCGGATGACATTTTACGCATTCGCAAC
	ACAGCCTCTCACACCAAAAACGGGACCCCTCAGAAAGGCCACTCAAAAGTTGAGTTTAAC
	CTGAATGATTGTCATAAGATGATTGATTTCTTCAAATCATCAATTCAGAAACACCCGGAA
	TGGGGGTCTTTTGGCTTTACGTTTTCTGATACCAGTGATTTTGAAGACATGAGTGCCTTC
	TACCGGGAAGTAGAAAACCAGGGTTACGTAATTAGCTTTGACAAAATCAAAGAGACCTAT
	ATACAGAGCCAGGTGGAACAGGGTAATCTCTACTTATTCCAGATTTATAACAAGGATTTC
	TCGCCCTACAGCAAAGGCAAACCAAACCTGCATACTCTGTACTGGAAAGCCCTGTTTGAA
	GAAGCGAACCTGAATAACGTAGTGGCGAAGTTGAACGGTGAAGCGGAAATCTTCTTCCGT
	CGTCACTCCATTAAGGCCTCTGATAAAGTTGTCCATCCGGCAAATCAGGCCATTGATAAT
	AAGAATCCACACACGGAAAAAACGCAGTCAACCTTTGAATATGACCTCGTTAAAGACAAA
	CGCTACACGCAAGATAAGTTCTTTTTCCACGTCCCAATCAGCCTCAACTTTAAAGCACAA
	GGGGTTTCAAAGTTTAATGATAAAGTCAATGGGTTCCTCAAGGGCAACCCGGATGTCAAC
	ATTATAGGTATAGACAGGGGCGAACGCCATCTGCTTTACTTTACCGTAGTGAATCAGAAA
	GGTGAAATACTGGTTCAGGAATCATTAAATACCTTGATGTCGGACAAAGGGCACGTTAAT
	GATTACCAGCAGAAACTGGATAAAAAAGAACAGGAACGTGATGCTGCGCGTAAATCGTGG
	ACCACGGTTGAGAACATTAAAGAGCTGAAAGAGGGGTATCTAAGCCATGTGGTACACAAA
	CTGGCGCACCTCATCATTAAATATAACGCAATAGTCTGCCTAGAAGACTTGAATTTTGGC
	TTTAAACGCGGCCGCTTCAAAGTGGAAAAACAAGTTTATCAAAAATTTGAAAAGGCGCTT
	ATAGATAAACTGAATTATCTGGTTTTTAAAGAAAAGGAACTTGGTGAGGTAGGGCACTAC
	TTGACAGCTTATCAACTGACGGCCCCGTTCGAATCATTCAAAAAACTGGGCAAACAGTCT
	GGCATTCTGTTTTACGTGCCGGCAGATTATACTTCAAAAATCGATCCAACAACTGGCTTT
	GTGAACTTCCTGGACCTGAGATATCAGTCTGTAGAAAAAGCTAAACAACTTCTTAGCGAT
	TTTAATGCCATTCGTTTTAACAGCGTTCAGAATTACTTTGAATTCGAAATTGACTATAAA
	AAACTTACTCCGAAACGTAAAGTCGGAACCCAAAGTAAATGGGTAATTTGTACGTATGGC
	GATGTCAGGTATCAGAACCGTCGGAATCAAAAAGGTCATTGGGAGACCGAAGAAGTGAAC
	GTGACCGAAAAGCTGAAGGCTCTGTTCGCCAGCGATTCAAAAACTACAACTGTGATCGAT
	TACGCAAATGATGATAACCTGATAGATGTGATTTTAGAGCAGGATAAAGCCAGCTTTTTT
	AAAGAACTGTTGTGGCTCCTGAAACTTACGATGACCTTACGACATTCCAAGATCAAATCG
	GAAGATGATTTTATTCTGTCACCGGTCAAGAATGAGCAGGGTGAATTCTATGATAGTAGG
	AAAGCCGGCGAAGTGTGGCCGAAAGACGCCGACGCCAATGGCGCCTATCATATCGCGCTC
	AAAGGGCTTTGGAATTTGCAGCAGATTAACCAGTGGGAAAAAGGTAAAACCCTGAATCTG
	GCTATCAAAAACCAGGATTGGTTTAGCTTTATCCAAGAGAAACCGTATCAGGAATGA

SEQ	ATGCATACAGGCGGTCTTCTTAGTATGGACGCGAAAGAGTTCACAGGTCAGTATCCGTTG
ID	TCGAAAACATTACGATTCGAACTTCGGCCCATCGGCCGCACGTGGGATAACCTGGAGGCC
NO:	TCAGGCTACTTAGCGGAAGACCGCCATCGTGCCGAATGTTATCCTCGTGCGAAAGAGTTA
45	TTGGATGACAACCATCGTGCCTTCCTGAATCGTGTGTTGCCACAAATCGATATGGATTGG
	CACCCGATTGCGGAGGCCTTTTGTAAGGTACATAAAAACCCTGGTAATAAAGAACTTGCC
	CAGGATTACAACCTTCAGTTGTCAAAGCGCCGTAAGGAGATCAGCGCATATCTTCAGGAT
	GCAGATGGCTATAAAGGCCTGTTCGCGAAGCCCGCCTTAGACGAAGCTATGAAAATTGCG
	AAAGAAAACGGGAACGAAAGTGATATTGAGGTTCTCGAAGCGTTTAACGGTTTTAGCGTA
	TACTTCACCGGTTATCATGAGTCACGCGAGAACATTTATAGCGATGAGGATATGGTGAGC
	GTAGCCTACCGAATTACTGAGGATAATTTCCCGCGCTTTGTCTCAAACGCTTTGATCTTT
	GATAAATTAAACGAAAGCCATCCGGATATTATCTCTGAAGTATCGGGCAATCTTGGAGTT
	GATGACATTGGTAAGTACTTTGACGTGTCGAACTATAACAATTTTCTTTCCCAGGCCGGT
	ATAGATGACTACAATCACATTATTGGCGGCCATACAACCGAAGACGGACTGATACAAGCG
	TTTAATGTCGTATTGAACTTACGTCACCAAAAAGACCCTGGCTTTGAAAAAATTCAGTTC
	AAACAGCTCTACAAACAAATCCTGAGCGTGCGTACCAGCAAAAGCTACATCCCGAAACAG
	TTTGACAACTCTAAGGAGATGGTTGACTGCATTTGCGATTATGTCAGCAAAATAGAGAAA
	TCCGAAACAGTAGAACGGGCCCTGAAACTAGTCCGTAATATCAGTTCTTTCGACTTGCGC
	GGGATCTTTGTCAATAAAAAGAACTTGCGCATACTGAGCAACAAACTGATAGGAGATTGG
	GACGCGATCGAAACCGCATTGATGCATAGTTCTTCATCAGAAAACGATAAGAAAAGCGTA
	TATGATAGCGCGGAGGCTTTTACGTTGGATGACATCTTTTCAAGCGTGAAAAAATTTTCT
	GATGCCTCTGCCGAAGATATTGGCAACAGGGCGGAAGACATCTGTAGAGTGATAAGTGAG
	ACGGCCCCTTTTATCAACGATCTGCGAGCGGTGGACCTGGATAGCCTGAACGACGATGGT
	TATGAAGCGGCCGTCTCAAAAATTCGGGAGTCGCTGGAGCCTTATATGGATCTTTTCCAT
	GAACTGGAAATTTTCTCGGTTGGCGATGAGTTCCCAAAATGCGCAGCATTTTACAGCGAA
	CTGGAGGAAGTCAGCGAACAGCTGATCGAAATTATTCCGTTATTCAACAAGGCGCGTTCG
	TTCTGCACCCGGAAACGCTATAGCACCGATAAGATTAAAGTGAACTTAAAATTCCCGACC
	TTGGCGGACGGGTGGGACCTGAACAAAGAGAGAGACAACAAAGCCGCGATTCTGCGGAAA
	GACGGTAAGTATTATCTGGCAATTCTGGATATGAAGAAAGATCTGTCAAGCATTAGGACC
	AGCGACGAAGATGAATCCAGCTTCGAAAAGATGGAGTATAAACTGTTACCGAGTCCAGTA
	AAAATGCTGCCAAAGATATTCGTAAAATCGAAAGCCGCTAAGGAAAAATATGGCCTGACA
	GATCGTATGCTTGAATGCTACGATAAAGGTATGCATAAGTCGGGTAGTGCGTTTGATCTT
	GGCTTTTGCCATGAACTCATTGATTATTACAAGCGTTGTATCGCGGAGTACCCAGGCTGG
	GATGTGTTCGATTTCAAGTTTCGCGAAACTTCCGATTATGGGTCCATGAAAGAGTTCAAT
	GAAGATGTGGCCGGAGCCGGTTACTATATGAGTCTGAGAAAAATTCCGTGCAGCGAAGTG
	TACCGTCTGTTAGACGAGAAATCGATTTATCTATTTCAAATTTATAACAAAGATTACTCT
	GAAAATGCACATGGTAATAAGAACATGCATACCATGTACTGGGAGGGTCTCTTTTCCCCG
	CAAAACCTGGAGTCGCCCGTTTTCAAGTTGTCGGGTGGGGCAGAACTTTTCTTTCGAAAA
	TCCTCAATCCCTAACGATGCCAAAACAGTACACCCGAAAGGCTCAGTGCTGGTTCCACGT
	AATGATGTTAACGGTCGGCGTATTCCAGATTCAATCTACCGCGAACTGACACGCTATTTT
	AACCGTGGCGATTGCCGAATCAGTGACGAAGCCAAAAGTTATCTTGACAAGGTTAAGACT
	AAAAAAGCGGACCATGACATTGTGAAAGATCGCCGCTTTACCGTGGATAAAATGATGTTC
	CACGTCCCGATTGCGATGAACTTTAAGGCGATCAGTAAACCGAACTTAAACAAAAAAGTC
	ATTGATGGCATCATTGATGATCAGGATCTGAAAATCATTGGTATTGATCGTGGCGAGCGG
	AACTTAATTTACGTCACGATGGTTGACAGAAAAGGGAATATCTTATATCAGGATTCTCTT
	AACATCCTCAATGGCTACGACTATCGTAAAGCTCTGGATGTGCGCGAATATGACAACAAG
	GAAGCGCGTCGTAACTGGACTAAAGTGGAGGGCATTCGCAAAATGAAGGAAGGCTATCTG
	TCATTAGCGGTCTCGAAATTAGCGGATATGATTATCGAAAATAACGCCATCATCGTTATG
	GAGGACCTGAACCACGGATTCAAAGCGGGCCGCTCAAAGATTGAAAAACAAGTTTATCAG
	AAATTTGAGAGTATGCTGATTAACAAACTGGGCTATATGGTGTTAAAAGACAAGTCAATT
	GACCAATCAGGTGGCGCGCTGCATGGATACCAGCTGGCGAACCATGTTACCACCTTAGCA
	TCAGTTGGAAAGCAGTGTGGGGTTATCTTTTATATACCGGCAGCGTTCACTAGTAAAATA
	GATCCGACCACTGGTTTCGCCGATCTCTTTGCCCTGAGTAACGTTAAAAACGTAGCGAGC
	ATGCGTGAATTCTTTTCCAAAATGAAATCTGTCATTTATGATAAAGCTGAAGGCAAATTC
	GCATTCACCTTTGATTACTTGGATTACAACGTGAAGAGCGAATGTGGTCGTACGCTGTGG
	ACCGTTTACACCGTTGGTGAGCGCTTCACCTATTCCCGTGTGAACCGCGAATATGTACGT
	AAAGTCCCCACCGATATTATCTATGATGCCCTCCAGAAAGCAGGCATTAGCGTCGAAGGA
	GACTTAAGGGACAGAATTGCCGAAAGCGATGGCGATACGCTGAAGTCTATTTTTTACGCA
	TTCAAATACGCGCTAGATATGCGCGTTGAGAATCGCGAGGAAGACTACATTCAATCACCT
	GTGAAAAATGCCTCTGGGGAATTTTTTTGTTCAAAAAATGCTGGTAAAAGCCTCCCACAA
	GATAGCGATGCAAACGGTGCATATAACATTGCCCTGAAAGGTATTCTTCAATTACGCATG
	CTGTCTGAGCAGTACGACCCCAACGCGGAATCTATTAGACTTCCGCTGATAACCAATAAA
	GCCTGGCTGACATTCATGCAGTCTGGCATGAAGACCTGGAAAAATTAG

SEQ	ATGGATAGTTTAAAAGATTTTACGAATCTATATCCCGTAAGCAAAACTCTTCGTTTTGAA
ID	CTGAAACCTGTTGGAAAAACGTTGGAGAATATCGAGAAAGCGGGCATCCTGAAAGAAGAC
NO:	GAGCACCGTGCCGAAAGCTACAGGCGTGTCAAAAAGATTATCGATACTTATCACAAAGTG
46	TTCATTGATAGCAGTCTGGAGAACATGGCAAAAATGGGCATAGAAAATGAAATCAAAGCA
	ATGCTGCAGAGCTTTTGCGAGCTCTACAAGAAAGATCACCGAACGGAAGGTGAAGATAAA
	GCACTGGACAAAATTCGCGCCGTTCTTCGCGGTCTGATTGTTGGCGCGTTCACCGGCGTG
	TGCGGCCGCCGTGAAAACACCGTGCAGAACGAAAAGTACGAGTCGCTGTTCAAAGAAAAA
	CTGATAAAAGAAATTTTGCCTGACTTTGTGCTTTCGACCGAAGCGGAATCCCTGCCATTT
	TCTGTCGAAGAAGCGACCCGCAGCCTGAAAGAATTTGACTCATTCACAAGTTACTTTGCA
	GGCTTCTACGAAAACCGTAAAAACATCTACAGCACGAAGCCACAGAGCACGGCTATTGCT
	TATCGCCTGATTCATGAGAACCTGCCGAAGTTCATCGATAACATCCTTGTTTTTCAAAAA
	ATTAAAGAGCCGATTGCGAAAGAGTTAGAACATATTCGAGCTGACTTTTCTGCGGGTGGG
	TACATTAAAAAAGATGAGCGGCTGGAAGACATCTTCAGTCTAAACTATTATATCCACGTT
	CTGTCGCAGGCAGGCATTGAGAAATATAATGCGCTGATTGGTAAGATTGTCACAGAAGGC
	GATGGTGAGATGAAAGGTCTTAATGAACATATCAATCTGTATAACCAGCAGCGTGGTCGC
	GAAGACCGTCTTCCACTGTTCCGCCCACTGTATAAACAGATCCTGTCTGACCGGGAACAG
	CTGTCCTACCTGCCGGAAAGCTTTGAAAAGGATGAAGAGCTACTTCGCGCATTAAAGGAG
	TTTTACGACCATATTGCGGAAGACATTTTGGGTAGAACGCAGCAACTGATGACGTCAATT
	TCTGAATACGATCTGAGTAGAATCTACGTTAGGAATGATAGCCAGCTGACCGATATTAGC
	AAAAAAATGCTGGGCGACTGGAACGCTATCTATATGGCACGTGAACGTGCATATGATCAT
	GAACAAGCACCGAAACGTATAACCGCGAAATATGAGCGTGATCGCATTAAGGCGCTAAAG
	GGAGAAGAAAGCATCTCACTCGCAAACCTGAACTCCTGTATCGCTTTCTTAGATAACGTG
	CGCGATTGTCGCGTCGACACGTATCTGTCAACCCTTGGGCAGAAAGAGGGTCCACATGGT
	CTGTCTAACCTGGTGGAAAATGTCTTTGCGAGTTACCATGAAGCGGAACAACTGCTGTCT
	TTTCCATACCCCGAAGAAAACAATCTAATACAGGATAAAGATAACGTGGTGTTAATCAAA
	AACCTGCTGGACAACATCAGCGATCTGCAACGTTTCCTGAAACCTTTGTGGGGTATGGGT
	GACGAGCCAGACAAAGACGAACGTTTTTATGGTGAGTATAATTATATACGTGGCGCCCTT
	GACCAAGTTATTCCGCTGTATAACAAAGTACGGAACTATCTGACCCGTAAGCCATATTCT
	ACCCGTAAAGTGAAACTGAACTTTGGCAACTCGCAACTGCTGTCGGGTTGGGATCGTAAC
	AAAGAAAAAGATAATAGTTGTGTTATCCTGCGTAAGGGACAAAATTTTTACCTCGCGATT
	ATGAACAACAGACACAAGCGTTCATTTGAAAATAAGGTTCTGCCGGAGTATAAAGAGGGC
	GAACCGTACTTCGAGAAAATGGATTATAAGTTCTTACCAGACCCTAATAAGATGTTACCG
	AAAGTCTTTCTTTCGAAAAAAGGCATAGAAATCTATAAGCCGTCCCCGAAATTACTCGAA
	CAGTATGGGCACGGGACCCACAAGAAAGGGGATACTTTTAGCATGGACGATCTGCACGAA
	CTGATCGATTTTTTTAAACACTCCATCGAAGCCCATGAAGACTGGAAACAGTTTGGGTTC
	AAGTTCTCTGATACAGCCACATACGAGAATGTGTCTAGTTTTTATCGGGAAGTGGAGGAT
	CAGGGCTACAAACTTAGTTTTCGTAAAGTTTCAGAGAGTTATGTTTATAGTTTAATTGAT
	CAGGGAAAACTTTACCTGTTCCAGATCTACAACAAAGATTTCTCGCCATGTAGTAAGGGT
	ACCCCGAATCTGCATACACTCTATTGGAGAATGTTATTCGATGAGCGTAACTTAGCGGAT
	GTCATTTATAAATTGGACGGGAAAGCAGAGATCTTTTTTCGTGAAAAATCACTGAAGAAT
	GACCACCCGACTCATCCGGCCGGGAAACCGATCAAAAAAAAATCCCGCCAGAAAAAAGGA
	GAAGAGTCTCTGTTTGAATATGATCTGGTGAAAGACCGTCATTACACTATGGATAAATTT
	CAATTTCATGTTCCAATTACAATGAACTTCAAATGTTCGGCGGGTTCCAAAGTAAATGAT
	ATGGTAAACGCCCATATTCGCGAAGCGAAAGATATGCATGTTATTGGCATCGATAGAGGC
	GAAAGAAACCTGCTTTATATTTGCGTAATTGACAGCCGTGGTACCATTCTGGACCAGATC
	TCTTTAAACACCATCAATGACATCGATTATCACGACCTGTTGGAGTCTCGGGACAAGGAC
	CGCCAGCAGGAGCGCCGTAATTGGCAGACAATTGAAGGCATAAAAGAATTAAAACAGGGT
	TACCTTTCCCAGGCCGTACACCGCATAGCGGAACTGATGGTGGCCTACAAAGCCGTAGTT
	GCCCTGGAAGACTTGAATATGGGGTTTAAACGTGGCCGTCAAAAAGTCGAGAGCAGCGTG
	TATCAGCAATTTGAAAAACAGTTGATTGACAAGTTGAATTATTTGGTTGATAAAAAGAAA
	CGTCCAGAAGATATTGGTGGCTTACTGCGTGCATACCAGTTTACGGCACCTTTTAAGTCC
	TTCAAAGAAATGGGTAAACAGAACGGGTTTCTGTTTTACATCCCGGCCTGGAATACATCC
	AACATCGATCCTACCACCGGGTTTGTCAACCTGTTTCATGCACAATATGAAAACGTGGAT
	AAAGCGAAGAGTTTTTTCCAAAAATTCGATAGTATTTCGTATAACCCAAAAAAAGATTGG
	TTTGAGTTTGCGTTCGATTATAAAAATTTTACTAAAAAGGCTGAGGGATCCCGCAGTATG
	TGGATCCTCTGCACCCATGGCAGTCGTATTAAAAATTTTCGTAATTCGCAAAAGAATGGC
	CAGTGGGACTCGGAAGAGTTTGCCCTGACCGAAGCGTTCAAATCGCTGTTTGTACGCTAC
	GAAATTGACTACACAGCAGATCTGAAAACAGCCATCGTCGATGAAAAACAGAAAGATTTT
	TTTGTAGATCTCCTAAAACTGTTCAAACTGACTGTTCAGATGCGCAATTCCTGGAAAGAG
	AAAGACCTGGATTATCTGATTAGCCCGGTAGCCGGTGCTGATGGACGATTTTTCGATACT
	CGTGAAGGTAACAAAAGTCTCCCGAAAGATGCTGATGCCAATGGTGCATACAATATTGCA
	TTAAAGGGGCTATGGGCCTTGCGACAGATCCGCCAGACCAGCGAAGGCGGCAAGCTGAAA
	TTGGCCATATCGAATAAGGAATGGTTACAATTTGTTCAGGAACGTAGCTATGAAAAAGAT
	TGA

SEQ	ATGAACAACGGCACAAATAATTTTCAGAACTTCATCGGGATCTCAAGTTTGCAGAAAACG
ID	CTGCGCAATGCTCTGATCCCCACGGAAACCACGCAACAGTTCATCGTCAAGAACGGAATA
NO:	ATTAAAGAAGATGAGTTACGTGGCGAGAACCGCCAGATTCTGAAAGATATCATGGATGAC
47	TACTACCGCGGATTCATCTCTGAGACTCTGAGTTCTATTGATGACATAGATTGGACTAGC
	CTGTTCGAAAAAATGGAAATTCAGCTGAAAAATGGTGATAATAAAGATACCTTAATTAAG
	GAACAGACAGAGTATCGGAAAGCAATCCATAAAAAATTTGCGAACGACGATCGGTTTAAG
	AACATGTTTAGCGCCAAACTGATTAGTGACATATTACCTGAATTTGTCATCCACAACAAT
	AATTATTCGGCATCAGAGAAAGAGGAAAAAACCCAGGTGATAAAATTGTTTTCGCGCTTT
	GCGACTAGCTTTAAAGATTACTTCAAGAACCGTGCAAATTGCTTTTCAGCGGACGATATT
	TCATCAAGCAGCTGCCATCGCATCGTCAACGACAATGCAGAGATATTCTTTTCAAATGCG
	CTGGTCTACCGCCGGATCGTAAAATCGCTGAGCAATGACGATATCAACAAAATTTCGGGC
	GATATGAAAGATTCATTAAAAGAAATGAGTCTGGAAGAAATATATTCTTACGAGAAGTAT
	GGGGAATTTATTACCCAGGAAGGCATTAGCTTCTATAATGATATCTGTGGGAAAGTGAAT
	TCTTTTATGAACCTGTATTGTCAGAAAAATAAAGAAAACAAAAATTTATACAAACTTCAG
	AAACTTCACAAACAGATTCTATGCATTGCGGACACTAGCTATGAGGTCCCGTATAAATTT
	GAAAGTGACGAGGAAGTGTACCAATCAGTTAACGGCTTCCTTGATAACATTAGCAGCAAA
	CATATAGTCGAAAGATTACGCAAAATCGGCGATAACTATAACGGCTACAACCTGGATAAA
	ATTTATATCGTGTCCAAATTTTACGAGAGCGTTAGCCAAAAAACCTACCGCGACTGGGAA
	ACAATTAATACCGCCCTCGAAATTCATTACAATAATATCTTGCCGGGTAACGGTAAAAGT
	AAAGCCGACAAAGTAAAAAAAGCGGTTAAGAATGATTTACAGAAATCCATCACCGAAATA
	AATGAACTAGTGTCAAACTATAAGCTGTGCAGTGACGACAACATCAAAGCGGAGACTTAT
	ATACATGAGATTAGCCATATCTTGAATAACTTTGAAGCACAGGAATTGAAATACAATCCG
	GAAATTCACCTAGTTGAATCCGAGCTCAAAGCGAGTGAGCTTAAAAACGTGCTGGACGTG
	ATCATGAATGCGTTTCATTGGTGTTCGGTTTTTATGACTGAGGAACTTGTTGATAAAGAC
	AACAATTTTTATGCGGAACTGGAGGAGATTTACGATGAAATTTATCCAGTAATTAGTCTG
	TACAACCTGGTTCGTAACTACGTTACCCAGAAACCGTACAGCACGAAAAAGATTAAATTG
	AACTTTGGAATACCGACGTTAGCAGACGGTTGGTCAAAGTCCAAAGAGTATTCTAATAAC
	GCTATCATACTGATGCGCGACAATCTGTATTATCTGGGCATCTTTAATGCGAAGAATAAA
	CCGGACAAGAAGATTATCGAGGGTAATACGTCAGAAAATAAGGGTGACTACAAAAAGATG
	ATTTATAATTTGCTCCCGGGTCCCAACAAAATGATCCCGAAAGTTTTCTTGAGCAGCAAG
	ACGGGGGTGGAAACGTATAAACCGAGCGCCTATATCCTAGAGGGGTATAAACAGAATAAA
	CATATCAAGTCTTCAAAAGACTTTGATATCACTTTCTGTCATGATCTGATCGACTACTTC
	AAAAACTGTATTGCAATTCATCCCGAGTGGAAAAACTTCGGTTTTGATTTTAGCGACACC
	AGTACTTATGAAGACATTTCCGGGTTTTATCGTGAGGTAGAGTTACAAGGTTACAAGATT
	GATTGGACATACATTAGCGAAAAAGACATTGATCTGCTGCAGGAAAAAGGTCAACTGTAT
	CTGTTCCAGATATATAACAAAGATTTTTCGAAAAAATCAACCGGGAATGACAACCTTCAC
	ACCATGTACCTGAAAAATCTTTTCTCAGAAGAAAATCTTAAGGATATCGTCCTGAAACTT
	AACGGCGAAGCGGAAATCTTCTTCAGGAAGAGCAGCATAAAGAACCCAATCATTCATAAA
	AAAGGCTCGATTTTAGTCAACCGTACCTACGAAGCAGAAGAAAAAGACCAGTTTGGCAAC
	ATTCAAATTGTGCGTAAAAATATTCCGGAAAACATTTATCAGGAGCTGTACAAATACTTC
	AACGATAAAAGCGACAAAGAGCTGTCTGATGAAGCAGCCAAACTGAAGAATGTAGTGGGA
	CACCACGAGGCAGCGACGAATATAGTCAAGGACTATCGCTACACGTATGATAAATACTTC
	CTTCATATGCCTATTACGATCAATTTCAAAGCCAATAAAACGGGTTTTATTAATGATAGG
	ATCTTACAGTATATCGCTAAAGAAAAAGACTTACATGTGATCGGCATTGATCGGGGCGAG
	CGTAACCTGATCTACGTGTCCGTGATTGATACTTGTGGTAATATAGTTGAACAGAAAAGC
	TTTAACATTGTAAACGGCTACGACTATCAGATAAAACTGAAACAACAGGAGGGCGCTAGA
	CAGATTGCGCGGAAAGAATGGAAAGAAATTGGTAAAATTAAAGAGATCAAAGAGGGCTAC
	CTGAGCTTAGTAATCCACGAGATCTCTAAAATGGTAATCAAATACAATGCAATTATAGCG
	ATGGAGGATTTGTCTTATGGTTTTAAAAAAGGGCGCTTTAAGGTCGAACGGCAAGTTTAC
	CAGAAATTTGAAACCATGCTCATCAATAAACTCAACTATCTGGTATTTAAAGATATTTCG
	ATTACCGAGAATGGCGGTCTCCTGAAAGGTTATCAGCTGACATACATTCCTGATAAACTT
	AAAAACGTGGGTCATCAGTGCGGCTGCATTTTTTATGTGCCTGCTGCATACACGAGCAAA
	ATTGATCCGACCACCGGCTTTGTGAATATCTTTAAATTTAAAGACCTGACAGTGGACGCA
	AAACGTGAATTCATTAAAAAATTTGACTCAATTCGTTATGACAGTGAAAAAAATCTGTTC
	TGCTTTACATTTGACTACAATAACTTTATTACGCAAAACACGGTCATGAGCAAATCATCG
	TGGAGTGTGTATACATACGGCGTGCGCATCAAACGTCGCTTTGTGAACGGCCGCTTCTCA
	AACGAAAGTGATACCATTGACATAACCAAAGATATGGAGAAAACGTTGGAAATGACGGAC
	ATTAACTGGCGCGATGGCCACGATCTTCGTCAAGACATTATAGATTATGAAATTGTTCAG
	CACATATTCGAAATTTTCCGTTTAACAGTGCAAATGCGTAACTCCTTGTCTGAACTGGAG
	GACCGTGATTACGATCGTCTCATTTCACCTGTACTGAACGAAAATAACATTTTTTATGAC
	AGCGCGAAAGCGGGGGATGCACTTCCTAAGGATGCCGATGCAAATGGTGCGTATTGTATT
	GCATTAAAAGGGTTATATGAAATTAAACAAATTACCGAAAATTGGAAAGAAGATGGTAAA
	TTTTCGCGCGATAAACTCAAAATCAGCAATAAAGATTGGTTCGACTTTATCCAGAATAAG
	CGCTATCTCTAA

SEQ	ATGACCAATAAATTCACTAACCAGTATTCTCTCTCTAAGACCCTGCGCTTTGAACTGATT
ID	CCGCAGGGGAAAACCTTGGAGTTCATTCAAGAAAAAGGCCTCTTGTCTCAGGATAAACAG
NO:	AGGGCTGAATCTTACCAAGAAATGAAGAAAACTATTGATAAGTTTCATAAATATTTCATT
48	GATTTAGCCTTGTCTAACGCCAAATTAACTCACTTGGAAACGTATCTGGAGTTATACAAC
	AAATCTGCCGAAACTAAGAAAGAACAGAAATTTAAAGACGATTTGAAAAAAGTACAGGAC
	AATCTGCGTAAAGAAATTGTCAAATCCTTCAGTGACGGCGATGCTAAAAGCATTTTTGCC
	ATTCTGGACAAAAAAGAGTTGATTACTGTGGAATTAGAAAAGTGGTTTGAAAACAATGAG
	CAGAAAGACATCTACTTCGATGAGAAATTCAAAACTTTCACCACCTATTTTACAGGATTT
	CATCAAAACCGGAAGAACATGTACTCAGTAGAACCGAACTCCACGGCCATTGCGTATCGT
	TTGATCCATGAGAATCTGCCTAAATTTCTGGAGAATGCGAAAGCCTTTGAAAAGATTAAG
	CAGGTCGAATCGCTGCAAGTGAATTTTCGTGAACTCATGGGCGAATTTGGTGACGAAGGT
	CTAATCTTCGTTAACGAACTGGAAGAAATGTTTCAGATTAATTACTACAATGACGTGCTA
	TCGCAGAACGGTATCACAATCTACAATAGTATTATCTCAGGGTTCACAAAAAACGATATA
	AAATACAAAGGCCTGAACGAGTATATCAATAACTACAACCAAACAAAGGACAAAAAGGAT
	AGGCTTCCGAAACTGAAGCAGTTATACAAACAGATTTTATCTGACAGAATCTCCCTGAGC
	TTTCTGCCGGATGCTTTCACTGATGGGAAGCAGGTTCTGAAAGCGATTTTCGATTTTTAT
	AAGATTAACTTACTGAGCTACACGATTGAAGGTCAAGAAGAATCTCAAAACTTACTGCTC
	TTGATCCGTCAAACCATTGAAAATCTATCATCGTTCGATACGCAGAAAATCTACCTCAAA
	AACGATACTCACCTGACTACGATCTCTCAGCAGGTTTTCGGGGATTTTAGTGTATTTTCA
	ACAGCTCTGAACTACTGGTATGAAACCAAAGTCAATCCGAAATTCGAGACGGAATATTCT
	AAGGCCAACGAAAAAAAACGTGAGATTCTTGATAAAGCTAAAGCCGTATTTACTAAACAG
	GATTACTTTTCTATTGCTTTCCTGCAGGAAGTTTTATCGGAGTATATCCTGACCCTGGAT
	CATACATCTGATATCGTTAAAAAACACAGCAGCAATTGCATCGCTGACTATTTCAAAAAC
	CACTTTGTCGCCAAAAAAGAAAACGAAACAGACAAGACTTTCGATTTCATTGCTAACATC
	ACCGCAAAATACCAGTGTATTCAGGGTATCTTGGAAAACGCCGACCAATACGAAGACGAA
	CTGAAACAAGATCAGAAGCTGATCGATAATTTAAAATTCTTCTTAGATGCAATCCTGGAG
	CTGCTGCACTTCATCAAACCGCTTCATTTAAAGAGCGAGTCCATTACCGAAAAGGACACC
	GCCTTCTATGACGTTTTTGAAAATTATTATGAAGCCCTCTCCTTGCTGACTCCGCTGTAT
	AATATGGTACGCAATTACGTAACCCAGAAACCATATTCTACCGAAAAAATTAAACTGAAC
	TTTGAAAACGCACAGCTGCTCAACGGTTGGGACGCGAATAAAGAAGGTGACTACCTCACC
	ACCATCCTGAAAAAAGATGGTAACTATTTTCTGGCAATTATGGATAAGAAACATAATAAA
	GCATTCCAGAAATTTCCTGAAGGGAAAGAAAATTACGAAAAGATGGTGTACAAACTCTTA
	CCTGGAGTTAACAAAATGTTGCCGAAAGTATTTTTTAGTAATAAGAACATCGCGTACTTT
	AACCCGTCCAAAGAACTGCTGGAAAATTATAAAAAGGAGACGCATAAGAAAGGGGATACC
	TTTAACCTGGAACATTGCCATACCTTAATAGACTTCTTCAAGGATTCCCTGAATAAACAC
	GAGGATTGGAAATATTTCGATTTTCAGTTTAGTGAGACCAAGTCATACCAGGATCTTAGC
	GGCTTTTATCGCGAAGTAGAACACCAAGGCTATAAAATTAACTTCAAAAACATCGACAGC
	GAATACATCGACGGTTTAGTTAACGAGGGCAAACTGTTTCTGTTCCAGATCTATTCAAAG
	GATTTTAGCCCGTTCTCTAAAGGCAAACCAAATATGCATACGTTGTACTGGAAAGCACTG
	TTTGAAGAGCAAAACCTGCAGAATGTGATTTATAAACTGAACGGCCAAGCTGAGATTTTT
	TTCCGTAAAGCCTCGATTAAACCGAAAAATATCATCCTTCATAAGAAGAAAATAAAGATC
	GCTAAAAAACACTTCATAGATAAAAAAACCAAAACCTCCGAAATAGTGCCTGTTCAAACA
	ATTAAGAACTTGAATATGTACTACCAGGGCAAGATATCGGAAAAGGAGTTGACTCAAGAC
	GATCTTCGCTATATCGATAACTTTTCGATTTTTAACGAAAAAAACAAGACGATCGACATC
	ATCAAAGATAAACGCTTCACTGTAGATAAGTTCCAGTTTCATGTGCCGATTACTATGAAC
	TTCAAAGCTACCGGGGGTAGCTATATCAACCAAACGGTGTTGGAATACCTGCAGAATAAC
	CCGGAAGTCAAAATCATTGGGCTGGACCGCGGAGAACGTCACCTTGTGTACTTGACCTTA
	ATCGATCAGCAAGGCAACATCTTAAAACAAGAATCGCTGAATACCATTACGGATTCAAAG
	ATTAGCACCCCGTATCATAAGCTGCTCGATAACAAGGAGAATGAGCGCGACCTGGCCCGT
	AAAAACTGGGGCACGGTGGAAAACATTAAGGAGTTAAAGGAGGGTTATATTTCCCAGGTA
	GTGCATAAGATCGCCACTCTCATGCTCGAGGAAAATGCGATCGTTGTCATGGAAGACTTA
	AACTTCGGATTTAAACGTGGGCGATTTAAAGTAGAGAAACAAATCTACCAGAAGTTAGAA
	AAAATGCTGATTGACAAATTAAATTACTTGGTCCTAAAAGACAAACAGCCGCAAGAATTG
	GGTGGATTATACAACGCCCTCCAACTTACCAATAAATTCGAAAGTTTTCAGAAAATGGGT
	AAACAGTCAGGCTTTCTTTTTTATGTTCCTGCGTGGAACACATCCAAAATCGACCCTACA
	ACCGGCTTCGTCAATTACTTCTATACTAAATATGAAAACGTCGACAAAGCAAAAGCATTC
	TTTGAAAAGTTCGAAGCAATACGTTTTAACGCTGAGAAAAAATATTTCGAGTTCGAAGTC
	AAGAAATACTCAGACTTTAACCCCAAAGCTGAGGGCACACAGCAAGCGTGGACAATCTGC
	ACCTACGGCGAGCGCATCGAAACGAAGCGTCAAAAAGATCAGAATAACAAATTTGTTTCA
	ACACCTATCAACCTGACCGAGAAGATTGAAGACTTCTTAGGTAAAAATCAGATTGTTTAT
	GGCGACGGTAACTGTATAAAATCTCAAATAGCCTCAAAGGATGATAAAGCATTTTTCGAA
	ACATTATTATATTGGTTCAAAATGACACTGCAGATGCGCAATAGTGAGACGCGTACAGAT
	ATTGATTATCTTATCAGCCCGGTCATGAACGACAACGGTACTTTTTACAACTCCAGAGAC
	TATGAAAAACTTGAGAATCCAACTCTCCCCAAAGATGCTGATGCGAACGGTGCTTATCAC
	ATCGCGAAAAAAGGTCTGATGCTGCTGAACAAAATCGACCAAGCCGATCTGACTAAGAAA
	GTTGACCTAAGCATTTCAAATCGGGACTGGTTACAGTTTGTTCAAAAGAACAAATGA

SEQ	ATGGAACAGGAATATTATCTGGGCTTGGACATGGGCACCGGTTCCGTCGGCTGGGCTGTT
ID	ACTGACAGTGAATATCACGTTCTAAGAAAGCATGGTAAGGCATTGTGGGGTGTAAGACTT
NO:	TTCGAATCTGCTTCCACTGCTGAAGAGCGTAGAATGTTTAGAACGAGTCGACGTAGGCTA
49	GACAGGCGCAATTGGAGAATCGAAATTTTACAAGAAATTTTTGCGGAAGAGATATCTAAG
	AAAGACCCAGGCTTTTTCCTGAGAATGAAGGAATCTAAGTATTACCCTGAGGATAAAAGA
	GATATAAATGGTAACTGTCCCGAATTGCCTTACGCATTATTTGTGGACGATGATTTTACC
	GATAAGGATTACCATAAAAAGTTCCCAACTATCTACCATTTACGCAAAATGTTAATGAAT
	ACAGAGGAAACCCCAGACATAAGACTAGTTTATCTGGCAATACACCATATGATGAAACAT
	AGAGGCCATTTCTTACTTTCCGGGGATATCAACGAAATCAAAGAGTTTGGTACCACATTT
	AGTAAGTTACTGGAAAACATAAAGAATGAAGAATTGGATTGGAACTTAGAACTCGGAAAA
	GAAGAATACGCGGTTGTCGAATCTATCCTGAAGGATAATATGCTGAATAGGTCGACCAAA
	AAAACTAGGCTGATCAAAGCACTGAAAGCCAAATCTATCTGCGAAAAAGCTGTTTTAAAT
	TTACTTGCTGGTGGCACTGTTAAGTTATCAGACATTTTTGGTTTGGAAGAATTGAACGAA
	ACCGAGCGTCCAAAAATTAGTTTCGCTGATAATGGCTACGATGATTACATTGGTGAGGTG
	GAAAACGAGTTGGGCGAACAATTTTATATTATAGAGACAGCTAAGGCAGTCTATGACTGG
	GCTGTTTTAGTAGAAATCCTTGGTAAATACACATCTATCTCCGAAGCGAAAGTTGCTACT
	TACGAAAAGCACAAGTCCGATCTCCAGTTTTTGAAGAAAATTGTCAGGAAATATCTGACT
	AAGGAAGAATATAAAGATATTTTCGTTAGTACCTCTGACAAACTGAAAAATTACTCCGCT
	TACATCGGGATGACCAAGATTAATGGCAAAAAAGTTGATCTGCAAAGCAAAAGGTGTTCG
	AAGGAAGAATTTTATGATTTCATTAAAAAGAATGTCTTAAAAAAATTAGAAGGTCAGCCA
	GAATACGAATATTTGAAAGAAGAACTGGAAAGAGAGACATTCTTACCAAAACAAGTCAAC
	AGAGATAATGGGGTAATTCCATATCAAATTCACCTCTACGAATTAAAAAAAATTTTAGGC
	AATTTACGCGATAAAATTGACCTTATCAAAGAAAATGAGGATAAGCTGGTTCAACTCTTT
	GAATTCAGAATACCCTATTATGTGGGCCCACTGAACAAGATTGATGACGGCAAAGAAGGT
	AAATTCACATGGGCCGTCCGCAAATCCAATGAAAAAATTTACCCATGGAACTTTGAAAAT
	GTAGTAGATATTGAAGCGTCTGCGGAGAAATTTATTCGAAGAATGACTAATAAATGCACT
	TACTTGATGGGAGAGGATGTTCTGCCTAAAGACAGCTTATTATACAGCAAGTACATGGTT
	CTAAACGAACTTAACAACGTTAAGTTGGACGGTGAGAAATTAAGTGTAGAATTGAAACAA
	AGATTGTATACTGACGTCTTCTGCAAGTACAGAAAAGTGACAGTTAAAAAAATTAAGAAT
	TACTTGAAGTGCGAAGGTATAATTTCTGGAAACGTAGAGATTACTGGTATTGATGGTGAT
	TTCAAAGCATCCCTAACAGCTTACCACGATTTCAAGGAAATCCTGACAGGAACTGAACTC
	GCAAAAAAAGATAAAGAAAACATTATTACTAATATTGTTCTTTTCGGTGATGACAAGAAA
	TTGTTGAAGAAAAGACTGAATAGACTTTACCCCCAGATTACTCCCAATCAACTTAAGAAA
	ATTTGTGCTTTGTCTTACACAGGATGGGGTCGTTTTTCAAAAAAGTTCTTAGAAGAGATT
	ACCGCACCTGATCCAGAAACAGGCGAAGTATGGAATATAATTACCGCCTTATGGGAATCG
	AACAATAATCTTATGCAACTTCTGAGCAATGAATATCGTTTCATGGAAGAAGTTGAGACT
	TACAACATGGGCAAACAGACGAAGACTTTATCCTATGAAACTGTGGAAAATATGTATGTA
	TCACCTTCTGTCAAGAGACAAATTTGGCAAACCTTAAAAATTGTCAAAGAATTAGAAAAG
	GTAATGAAGGAGTCTCCTAAACGTGTGTTTATTGAAATGGCTAGAGAAAAACAAGAGTCA
	AAAAGAACCGAGTCAAGAAAGAAGCAGTTAATCGATTTATATAAGGCTTGTAAAAACGAA
	GAGAAAGATTGGGTTAAAGAATTGGGGGACCAAGAGGAACAAAAACTACGGTCGGATAAG
	TTGTATTTATACTATACGCAAAAGGGACGATGTATGTATTCCGGCGAGGTAATAGAATTG
	AAGGATTTATGGGACAATACAAAATATGACATAGACCATATATATCCCCAATCAAAAACG
	ATGGACGATAGCTTGAACAATAGAGTACTCGTGAAAAAAAAATATAATGCGACCAAATCT
	GATAAGTATCCTCTGAATGAAAATATCAGACATGAAAGAAAGGGGTTCTGGAAGTCCTTG
	TTAGATGGTGGGTTTATAAGCAAAGAAAAGTACGAGCGTCTAATAAGAAACACGGAGTTA
	TCGCCAGAAGAACTCGCTGGTTTTATTGAGAGGCAAATCGTGGAAACGAGACAATCTACC
	AAAGCCGTTGCTGAGATCCTAAAGCAAGTTTTCCCAGAGTCGGAGATTGTCTATGTCAAA
	GCTGGCACAGTGAGCAGGTTTAGGAAAGACTTCGAACTATTAAAGGTAAGAGAAGTGAAC
	GATTTACATCACGCAAAGGACGCTTACCTAAATATCGTTGTAGGTAACTCATATTATGTT
	AAATTTACCAAGAACGCCTCTTGGTTTATAAAGGAGAACCCAGGTAGAACATATAACCTG
	AAAAAGATGTTCACCTCTGGTTGGAATATTGAGAGAAACGGAGAAGTCGCATGGGAAGTT
	GGTAAGAAAGGGACTATAGTGACAGTAAAGCAAATTATGAACAAAAATAATATCCTCGTT
	ACAAGGCAGGTTCATGAAGCAAAGGGCGGCCTTTTTGACCAACAAATTATGAAGAAAGGG
	AAAGGTCAAATTGCAATAAAAGAAACCGATGAGAGACTAGCGTCAATAGAAAAGTATGGT
	GGCTATAATAAAGCTGCGGGTGCATACTTTATGCTTGTTGAATCAAAAGACAAGAAAGGT
	AAGACTATTAGAACTATAGAATTTATACCCCTGTACCTTAAAAACAAAATTGAATCGGAT
	GAGTCAATCGCGTTAAATTTTCTAGAGAAAGGAAGGGGTTTAAAAGAACCAAAGATCCTG
	TTAAAAAAGATTAAGATTGACACCTTGTTCGATGTAGATGGATTTAAAATGTGGTTATCT
	GGCAGAACAGGCGATAGACTTTTGTTTAAGTGCGCTAATCAATTAATTTTGGATGAGAAA
	ATCATTGTCACAATGAAAAAAATAGTTAAGTTTATTCAGAGAAGACAAGAAAACAGGGAG
	TTGAAATTATCTGATAAAGATGGTATCGACAATGAAGTTTTAATGGAAATCTACAATACA
	TTCGTTGATAAACTTGAAAATACCGTATATCGAATCAGGTTAAGTGAACAAGCCAAAACA
	TTAATTGATAAACAAAAAGAATTTGAAAGGCTATCACTGGAAGACAAATCCTCCACCCTA
	TTTGAAATTTTGCATATATTCCAGTGCCAATCTTCAGCAGCTAATTTAAAAATGATTGGC
	GGACCTGGGAAAGCCGGCATCCTAGTGATGAACAATAATATCTCCAAGTGTAACAAAATA
	TCAATTATTAACCAATCTCCGACAGGTATTTTTGAAAATGAAATAGACTTGCTTAAGATA
	TAA

SEQ	ATGTCTTTCGACTCTTTCACCAACCTGTACTCTCTGTCTAAAACCCTGAAATTCGAAATG
ID	CGTCCGGTTGGTAACACCCAGAAAATGCTGGACAACGCGGGTGTTTTCGAAAAAGACAAA
NO:	CTGATCCAGAAAAAATACGGTAAAACCAAACCGTACTTCGACCGTCTGCACCGTGAATTC
50	ATCGAAGAAGCGCTGACCGGTGTTGAACTGATCGGTCTGGACGAAAACTTCCGTACCCTG
	GTTGACTGGCAGAAAGACAAAAAAAACAACGTTGCGATGAAAGCGTACGAAAACTCTCTG
	CAGCGTCTGCGTACCGAAATCGGTAAAATCTTCAACCTGAAAGCGGAAGACTGGGTTAAA
	AACAAATACCCGATCCTGGGTCTGAAAAACAAAAACACCGACATCCTGTTCGAAGAAGCG
	GTTTTCGGTATCCTGAAAGCGCGTTACGGTGAAGAAAAAGACACCTTCATCGAAGTTGAA
	GAAATCGACAAAACCGGTAAATCTAAAATCAACCAGATCTCTATCTTCGACTCTTGGAAA
	GGTTTCACCGGTTACTTCAAAAAATTCTTCGAAACCCGTAAAAACTTCTACAAAAACGAC
	GGTACCTCTACCGCGATCGCGACCCGTATCATCGACCAGAACCTGAAACGTTTCATCGAC
	AACCTGTCTATCGTTGAATCTGTTCGTCAGAAAGTTGACCTGGCGGAAACCGAAAAATCT
	TTCTCTATCTCTCTGTCTCAGTTCTTCTCTATCGACTTCTACAACAAATGCCTGCTGCAG
	GACGGTATCGACTACTACAACAAAATCATCGGTGGTGAAACCCTGAAAAACGGTGAAAAA
	CTGATCGGTCTGAACGAACTGATCAACCAGTACCGTCAGAACAACAAAGACCAGAAAATC
	CCGTTCTTCAAACTGCTGGACAAACAGATCCTGTCTGAAAAAATCCTGTTCCTGGACGAA
	ATCAAAAACGACACCGAACTGATCGAAGCGCTGTCTCAGTTCGCGAAAACCGCGGAAGAA
	AAAACCAAAATCGTTAAAAAACTGTTCGCGGACTTCGTTGAAAACAACTCTAAATACGAC
	CTGGCGCAGATCTACATCTCTCAGGAAGCGTTCAACACCATCTCTAACAAATGGACCTCT
	GAAACCGAAACCTTCGCGAAATACCTGTTCGAAGCGATGAAATCTGGTAAACTGGCGAAA
	TACGAAAAAAAAGACAACTCTTACAAATTCCCGGACTTCATCGCGCTGTCTCAGATGAAA
	TCTGCGCTGCTGTCTATCTCTCTGGAAGGTCACTTCTGGAAAGAAAAATACTACAAAATC
	TCTAAATTCCAGGAAAAAACCAACTGGGAACAGTTCCTGGCGATCTTCCTGTACGAATTC
	AACTCTCTGTTCTCTGACAAAATCAACACCAAAGACGGTGAAACCAAACAGGTTGGTTAC
	TACCTGTTCGCGAAAGACCTGCACAACCTGATCCTGTCTGAACAGATCGACATCCCGAAA
	GACTCTAAAGTTACCATCAAAGACTTCGCGGACTCTGTTCTGACCATCTACCAGATGGCG
	AAATACTTCGCGGTTGAAAAAAAACGTGCGTGGCTGGCGGAATACGAACTGGACTCTTTC
	TACACCCAGCCGGACACCGGTTACCTGCAGTTCTACGACAACGCGTACGAAGACATCGTT
	CAGGTTTACAACAAACTGCGTAACTACCTGACCAAAAAACCGTACTCTGAAGAAAAATGG
	AAACTGAACTTCGAAAACTCTACCCTGGCGAACGGTTGGGACAAAAACAAAGAATCTGAC
	AACTCTGCGGTTATCCTGCAGAAAGGTGGTAAATACTACCTGGGTCTGATCACCAAAGGT
	CACAACAAAATCTTCGACGACCGTTTCCAGGAAAAATTCATCGTTGGTATCGAAGGTGGT
	AAATACGAAAAAATCGTTTACAAATTCTTCCCGGACCAGGCGAAAATGTTCCCGAAAGTT
	TGCTTCTCTGCGAAAGGTCTGGAATTCTTCCGTCCGTCTGAAGAAATCCTGCGTATCTAC
	AACAACGCGGAATTCAAAAAAGGTGAAACCTACTCTATCGACTCTATGCAGAAACTGATC
	GACTTCTACAAAGACTGCCTGACCAAATACGAAGGTTGGGCGTGCTACACCTTCCGTCAC
	CTGAAACCGACCGAAGAATACCAGAACAACATCGGTGAATTCTTCCGTGACGTTGCGGAA
	GACGGTTACCGTATCGACTTCCAGGGTATCTCTGACCAGTACATCCACGAAAAAAACGAA
	AAAGGTGAACTGCACCTGTTCGAAATCCACAACAAAGACTGGAACCTGGACAAAGCGCGT
	GACGGTAAATCTAAAACCACCCAGAAAAACCTGCACACCCTGTACTTCGAATCTCTGTTC
	TCTAACGACAACGTTGTTCAGAACTTCCCGATCAAACTGAACGGTCAGGCGGAAATCTTC
	TACCGTCCGAAAACCGAAAAAGACAAACTGGAATCTAAAAAAGACAAAAAAGGTAACAAA
	GTTATCGACCACAAACGTTACTCTGAAAACAAAATCTTCTTCCACGTTCCGCTGACCCTG
	AACCGTACCAAAAACGACTCTTACCGTTTCAACGCGCAGATCAACAACTTCCTGGCGAAC
	AACAAAGACATCAACATCATCGGTGTTGACCGTGGTGAAAAACACCTGGTTTACTACTCT
	GTTATCACCCAGGCGTCTGACATCCTGGAATCTGGTTCTCTGAACGAACTGAACGGTGTT
	AACTACGCGGAAAAACTGGGTAAAAAAGCGGAAAACCGTGAACAGGCGCGTCGTGACTGG
	CAGGACGTTCAGGGTATCAAAGACCTGAAAAAAGGTTACATCTCTCAGGTTGTTCGTAAA
	CTGGCGGACCTGGCGATCAAACACAACGCGATCATCATCCTGGAAGACCTGAACATGCGT
	TTCAAACAGGTTCGTGGTGGTATCGAAAAATCTATCTACCAGCAGCTGGAAAAAGCGCTG
	ATCGACAAACTGTCTTTCCTGGTTGACAAAGGTGAAAAAAACCCGGAACAGGCGGGTCAC
	CTGCTGAAAGCGTACCAGCTGTCTGCGCCGTTCGAAACCTTCCAGAAAATGGGTAAACAG
	ACCGGTATCATCTTCTACACCCAGGCGTCTTACACCTCTAAATCTGACCCGGTTACCGGT
	TGGCGTCCGCACCTGTACCTGAAATACTTCTCTGCGAAAAAAGCGAAAGACGACATCGCG
	AAATTCACCAAAATCGAATTCGTTAACGACCGTTTCGAACTGACCTACGACATCAAAGAC
	TTCCAGCAGGCGAAAGAATACCCGAACAAAACCGTTTGGAAAGTTTGCTCTAACGTTGAA
	CGTTTCCGTTGGGACAAAAACCTGAACCAGAACAAAGGTGGTTACACCCACTACACCAAC
	ATCACCGAAAACATCCAGGAACTGTTCACCAAATACGGTATCGACATCACCAAAGACCTG
	CTGACCCAGATCTCTACCATCGACGAAAAACAGAACACCTCTTTCTTCCGTGACTTCATC
	TTCTACTTCAACCTGATCTGCCAGATCCGTAACACCGACGACTCTGAAATCGCGAAAAAA
	AACGGTAAAGACGACTTCATCCTGTCTCCGGTTGAACCGTTCTTCGACTCTCGTAAAGAC
	AACGGTAACAAACTGCCGGAAAACGGTGACGACAACGGTGCGTACAACATCGCGCGTAAA
	GGTATCGTTATCCTGAACAAAATCTCTCAGTACTCTGAAAAAAACGAAAACTGCGAAAAA
	ATGAAATGGGGTGACCTGTACGTTTCTAACATCGACTGGGACAACTTCGTT

SEQ	ATGGAAAACTTTAAAAACTTATACCCAATAAACAAAACGTTACGTTTTGAACTGCGTCCA
ID	TATGGTAAAACACTGGAAAACTTTAAAAAAAGCGGTTTGTTGGAGAAGGATGCATTTAAA
NO:	GCGAACTCTCGCAGATCCATGCAGGCCATCATTGATGAAAAATTTAAAGAGACGATCGAA
51	GAACGTCTGAAATACACGGAATTTAGTGAGTGTGACTTAGGTAATATGACTTCTAAAGAT
	AAGAAAATCACCGATAAGGCGGCGACCAACCTGAAGAAGCAAGTCATTTTATCTTTTGAT
	GATGAAATCTTTAACAACTATTTGAAACCGGACAAAAACATCGATGCCTTATTTAAAAAT
	GACCCTTCGAACCCGGTGATTAGCACATTTAAGGGCTTCACAACGTATTTTGTCAATTTT
	TTTGAAATTCGTAAACATATCTTCAAAGGAGAATCAAGCGGCTCTATGGCTTATCGCATT
	ATTGATGAAAACCTGACGACCTATTTGAATAACATTGAAAAAATCAAAAAACTGCCAGAG
	GAATTAAAGTCTCAGTTAGAAGGCATCGACCAGATCGACAAACTCAACAACTATAACGAA
	TTTATTACGCAGTCTGGTATCACCCACTATAATGAAATTATTGGAGGTATCAGTAAATCA
	GAAAATGTGAAAATCCAAGGGATTAATGAAGGCATTAACCTCTATTGCCAGAAAAATAAA
	GTGAAACTGCCGAGGCTGACTCCACTCTACAAAATGATCCTGTCTGACCGCGTCTCGAAT
	AGCTTTGTCCTGGACACAATTGAAAACGATACGGAATTGATTGAGATGATAAGCGATCTG
	ATTAACAAAACCGAAATTTCACAGGATGTAATCATGAGTGATATACAAAACATCTTTATT
	AAATATAAACAGCTTGGTAATCTGCCTGGAATTAGCTATTCGTCAATAGTGAACGCAATC
	TGTTCTGATTATGATAACAATTTTGGCGACGGTAAGCGTAAAAAGAGTTATGAAAACGAT
	AGGAAAAAACACCTGGAAACTAACGTGTATTCTATCAACTATATCAGCGAACTGCTTACG
	GACACCGATGTGAGTTCAAACATTAAGATGCGGTATAAGGAGCTTGAACAGAACTACCAG
	GTCTGTAAGGAAAACTTCAACGCAACCAACTGGATGAACATTAAAAATATCAAACAATCC
	GAGAAGACCAACTTAATCAAAGATCTGCTGGATATTTTGAAGAGCATTCAACGTTTTTAT
	GATCTGTTCGATATCGTTGATGAAGACAAGAATCCTAGTGCGGAATTTTATACATGGCTG
	TCTAAAAATGCGGAGAAATTGGATTTCGAATTCAATTCTGTTTATAATAAATCACGCAAC
	TATTTGACCCGCAAACAATACAGCGACAAAAAGATAAAACTAAACTTCGACAGTCCGACA
	TTGGCAAAGGGCTGGGACGCAAATAAGGAAATCGATAACTCTACGATAATTATGCGTAAG
	TTCAATAATGATCGAGGTGATTATGATTATTTCTTAGGCATTTGGAACAAAAGCACCCCG
	GCCAACGAAAAGATAATTCCACTGGAGGATAACGGTCTGTTCGAAAAAATGCAGTACAAA
	TTATATCCGGATCCAAGCAAGATGCTTCCAAAGCAGTTTCTGTCTAAAATTTGGAAAGCT
	AAGCATCCGACCACCCCAGAATTTGACAAGAAATATAAGGAAGGCCGCCATAAGAAAGGT
	CCCGATTTTGAAAAAGAATTCTTGCACGAACTGATTGATTGCTTTAAACATGGCTTAGTC
	AATCACGATGAAAAGTATCAAGATGTTTTTGGATTCAATTTGAGAAACACAGAAGACTAC
	AATTCCTACACTGAGTTTCTCGAAGATGTGGAACGATGTAATTATAATCTGAGCTTTAAC
	AAAATCGCGGACACCTCGAATCTGATTAACGATGGTAAACTTTATGTTTTCCAGATCTGG
	AGCAAGGATTTCTCTATTGACAGCAAAGGCACCAAAAACCTGAACACCATTTACTTTGAA
	AGTCTCTTCAGCGAAGAAAATATGATTGAGAAAATGTTTAAACTTAGCGGTGAAGCTGAA
	ATATTCTATCGCCCGGCAAGCCTGAACTATTGCGAAGACATTATCAAAAAGGGTCATCAC
	CACGCTGAACTGAAAGATAAATTTGATTATCCTATCATAAAAGATAAACGCTATAGCCAG
	GATAAATTTTTTTTTCATGTTCCTATGGTCATTAACTACAAATCAGAAAAACTGAACTCT
	AAAAGCCTCAATAATCGAACCAATGAAAACCTTGGGCAGTTTACCCATATAATTGGAATT
	GATCGCGGAGAGCGTCATTTAATCTACCTGACCGTAGTCGATGTATCGACCGGCGAGATC
	GTCGAGCAGAAGCACTTAGACGAGATTATCAACACTGATACCAAAGGTGTTGAGCATAAG
	ACGCACTATCTAAACAAGCTGGAGGAAAAATCGAAAACCCGTGATAATGAACGTAAGAGT
	TGGGAGGCAATTGAAACGATTAAAGAACTGAAGGAGGGTTATATCAGCCACGTAATCAAT
	GAAATTCAAAAACTGCAGGAAAAATACAACGCCCTGATCGTTATGGAAAATCTGAATTAC
	GGTTTCAAAAATTCTCGCATCAAAGTGGAAAAACAGGTATATCAGAAGTTCGAGACGGCA
	TTAATTAAAAAGTTTAATTACATCATTGACAAAAAAGATCCGGAAACTTATATTCATGGC
	TATCAGCTGACGAACCCGATCACCACACTGGATAAAATTGGTAACCAGTCTGGTATCGTG
	CTTTACATCCCTGCCTGGAATACCAGTAAAATCGATCCGGTAACGGGATTCGTCAACCTT
	CTATATGCAGATGACCTCAAATATAAGAATCAGGAACAGGCCAAGTCTTTTATTCAGAAA
	ATCGATAACATTTACTTTGAGAATGGGGAATTCAAATTTGATATTGATTTTTCTAAATGG
	AACAATCGTTATAGTATATCTAAGACGAAATGGACGCTCACCTCGTACGGAACCCGAATC
	CAGACATTCCGCAATCCGCAGAAGAACAATAAATGGGACAGCGCCGAGTATGATCTCACT
	GAAGAATTCAAATTGATTCTGAACATTGACGGTACCCTGAAAAGCCAGGATGTCGAAACC
	TATAAAAAATTTATGTCTCTGTTCAAGCTGATGCTGCAACTTAGGAACTCTGTTACCGGC
	ACTGATATCGATTATATGATCTCCCCTGTCACTGATAAAACAGGTACGCATTTCGATTCG
	CGCGAAAATATCAAAAATCTGCCCGCAGATGCCGACGCCAATGGGGCGTACAATATTGCA
	CGCAAGGGTATCATGGCGATCGAAAACATTATGAATGGTATCAGCGACCCGCTGAAAATC
	TCAAACGAAGATTATTTGAAATATATCCAAAACCAGCAGGAATAA

SEQ	ATGACCCAGTTCGAAGGTTTCACCAACCTGTACCAGGTTTCTAAAACCCTGCGTTTCGAA
ID	CTGATCCCGCAGGGTAAAACCCTGAAACACATCCAGGAACAGGGTTTCATCGAAGAAGAC
NO:	AAAGCGCGTAACGACCACTACAAAGAACTGAAACCGATCATCGACCGTATCTACAAAACC
52	TACGCGGACCAGTGCCTGCAGCTGGTTCAGCTGGACTGGGAAAACCTGTCTGCGGCGATC
	GACTCTTACCGTAAAGAAAAAACCGAAGAAACCCGTAACGCGCTGATCGAAGAACAGGCG
	ACCTACCGTAACGCGATCCACGACTACTTCATCGGTCGTACCGACAACCTGACCGACGCG
	ATCAACAAACGTCACGCGGAAATCTACAAAGGTCTGTTCAAAGCGGAACTGTTCAACGGT
	AAAGTTCTGAAACAGCTGGGTACCGTTACCACCACCGAACACGAAAACGCGCTGCTGCGT
	TCTTTCGACAAATTCACCACCTACTTCTCTGGTTTCTACGAAAACCGTAAAAACGTTTTC
	TCTGCGGAAGACATCTCTACCGCGATCCCGCACCGTATCGTTCAGGACAACTTCCCGAAA
	TTCAAAGAAAACTGCCACATCTTCACCCGTCTGATCACCGCGGTTCCGTCTCTGCGTGAA
	CACTTCGAAAACGTTAAAAAAGCGATCGGTATCTTCGTTTCTACCTCTATCGAAGAAGTT
	TTCTCTTTCCCGTTCTACAACCAGCTGCTGACCCAGACCCAGATCGACCTGTACAACCAG
	CTGCTGGGTGGTATCTCTCGTGAAGCGGGTACCGAAAAAATCAAAGGTCTGAACGAAGTT
	CTGAACCTGGCGATCCAGAAAAACGACGAAACCGCGCACATCATCGCGTCTCTGCCGCAC
	CGTTTCATCCCGCTGTTCAAACAGATCCTGTCTGACCGTAACACCCTGTCTTTCATCCTG
	GAAGAATTCAAATCTGACGAAGAAGTTATCCAGTCTTTCTGCAAATACAAAACCCTGCTG
	CGTAACGAAAACGTTCTGGAAACCGCGGAAGCGCTGTTCAACGAACTGAACTCTATCGAC
	CTGACCCACATCTTCATCTCTCACAAAAAACTGGAAACCATCTCTTCTGCGCTGTGCGAC
	CACTGGGACACCCTGCGTAACGCGCTGTACGAACGTCGTATCTCTGAACTGACCGGTAAA
	ATCACCAAATCTGCGAAAGAAAAAGTTCAGCGTTCTCTGAAACACGAAGACATCAACCTG
	CAGGAAATCATCTCTGCGGCGGGTAAAGAACTGTCTGAAGCGTTCAAACAGAAAACCTCT
	GAAATCCTGTCTCACGCGCACGCGGCGCTGGACCAGCCGCTGCCGACCACCCTGAAAAAA
	CAGGAAGAAAAAGAAATCCTGAAATCTCAGCTGGACTCTCTGCTGGGTCTGTACCACCTG
	CTGGACTGGTTCGCGGTTGACGAATCTAACGAAGTTGACCCGGAATTCTCTGCGCGTCTG
	ACCGGTATCAAACTGGAAATGGAACCGTCTCTGTCTTTCTACAACAAAGCGCGTAACTAC
	GCGACCAAAAAACCGTACTCTGTTGAAAAATTCAAACTGAACTTCCAGATGCCGACCCTG
	GCGTCTGGTTGGGACGTTAACAAAGAAAAAAACAACGGTGCGATCCTGTTCGTTAAAAAC
	GGTCTGTACTACCTGGGTATCATGCCGAAACAGAAAGGTCGTTACAAAGCGCTGTCTTTC
	GAACCGACCGAAAAAACCTCTGAAGGTTTCGACAAAATGTACTACGACTACTTCCCGGAC
	GCGGCGAAAATGATCCCGAAATGCTCTACCCAGCTGAAAGCGGTTACCGCGCACTTCCAG
	ACCCACACCACCCCGATCCTGCTGTCTAACAACTTCATCGAACCGCTGGAAATCACCAAA
	GAAATCTACGACCTGAACAACCCGGAAAAAGAACCGAAAAAATTCCAGACCGCGTACGCG
	AAAAAAACCGGTGACCAGAAAGGTTACCGTGAAGCGCTGTGCAAATGGATCGACTTCACC
	CGTGACTTCCTGTCTAAATACACCAAAACCACCTCTATCGACCTGTCTTCTCTGCGTCCG
	TCTTCTCAGTACAAAGACCTGGGTGAATACTACGCGGAACTGAACCCGCTGCTGTACCAC
	ATCTCTTTCCAGCGTATCGCGGAAAAAGAAATCATGGACGCGGTTGAAACCGGTAAACTG
	TACCTGTTCCAGATCTACAACAAAGACTTCGCGAAAGGTCACCACGGTAAACCGAACCTG
	CACACCCTGTACTGGACCGGTCTGTTCTCTCCGGAAAACCTGGCGAAAACCTCTATCAAA
	CTGAACGGTCAGGCGGAACTGTTCTACCGTCCGAAATCTCGTATGAAACGTATGGCGCAC
	CGTCTGGGTGAAAAAATGCTGAACAAAAAACTGAAAGACCAGAAAACCCCGATCCCGGAC
	ACCCTGTACCAGGAACTGTACGACTACGTTAACCACCGTCTGTCTCACGACCTGTCTGAC
	GAAGCGCGTGCGCTGCTGCCGAACGTTATCACCAAAGAAGTTTCTCACGAAATCATCAAA
	GACCGTCGTTTCACCTCTGACAAATTCTTCTTCCACGTTCCGATCACCCTGAACTACCAG
	GCGGCGAACTCTCCGTCTAAATTCAACCAGCGTGTTAACGCGTACCTGAAAGAACACCCG
	GAAACCCCGATCATCGGTATCGACCGTGGTGAACGTAACCTGATCTACATCACCGTTATC
	GACTCTACCGGTAAAATCCTGGAACAGCGTTCTCTGAACACCATCCAGCAGTTCGACTAC
	CAGAAAAAACTGGACAACCGTGAAAAAGAACGTGTTGCGGCGCGTCAGGCGTGGTCTGTT
	GTTGGTACCATCAAAGACCTGAAACAGGGTTACCTGTCTCAGGTTATCCACGAAATCGTT
	GACCTGATGATCCACTACCAGGCGGTTGTTGTTCTGGAAAACCTGAACTTCGGTTTCAAA
	TCTAAACGTACCGGTATCGCGGAAAAAGCGGTTTACCAGCAGTTCGAAAAAATGCTGATC
	GACAAACTGAACTGCCTGGTTCTGAAAGACTACCCGGCGGAAAAAGTTGGTGGTGTTCTG
	AACCCGTACCAGCTGACCGACCAGTTCACCTCTTTCGCGAAAATGGGTACCCAGTCTGGT
	TTCCTGTTCTACGTTCCGGCGCCGTACACCTCTAAAATCGACCCGCTGACCGGTTTCGTT
	GACCCGTTCGTTTGGAAAACCATCAAAAACCACGAATCTCGTAAACACTTCCTGGAAGGT
	TTCGACTTCCTGCACTACGACGTTAAAACCGGTGACTTCATCCTGCACTTCAAAATGAAC
	CGTAACCTGTCTTTCCAGCGTGGTCTGCCGGGTTTCATGCCGGCGTGGGACATCGTTTTC
	GAAAAAAACGAAACCCAGTTCGACGCGAAAGGTACCCCGTTCATCGCGGGTAAACGTATC
	GTTCCGGTTATCGAAAACCACCGTTTCACCGGTCGTTACCGTGACCTGTACCCGGCGAAC
	GAACTGATCGCGCTGCTGGAAGAAAAAGGTATCGTTTTCCGTGACGGTTCTAACATCCTG
	CCGAAACTGCTGGAAAACGACGACTCTCACGCGATCGACACCATGGTTGCGCTGATCCGT
	TCTGTTCTGCAGATGCGTAACTCTAACGCGGCGACCGGTGAAGACTACATCAACTCTCCG
	GTTCGTGACCTGAACGGTGTTTGCTTCGACTCTCGTTTCCAGAACCCGGAATGGCCGATG
	GACGCGGACGCGAACGGTGCGTACCACATCGCGCTGAAAGGTCAGCTGCTGCTGAACCAC
	CTGAAAGAATCTAAAGACCTGAAACTGCAGAACGGTATCTCTAACCAGGACTGGCTGGCG
	TACATCCAGGAACTGCGTAACTA

SEQ	ATGGCGGTTAAATCTATCAAAGTTAAACTGCGTCTGGACGACATGCCGGAAATCCGTGCG
ID	GGTCTGTGGAAACTGCACAAAGAAGTTAACGCGGGTGTTCGTTACTACACCGAATGGCTG
NO:	TCTCTGCTGCGTCAGGAAAACCTGTACCGTCGTTCTCCGAACGGTGACGGTGAACAGGAA
53	TGCGACAAAACCGCGGAAGAATGCAAAGCGGAACTGCTGGAACGTCTGCGTGCGCGTCAG
	GTTGAAAACGGTCACCGTGGTCCGGCGGGTTCTGACGACGAACTGCTGCAGCTGGCGCGT
	CAGCTGTACGAACTGCTGGTTCCGCAGGCGATCGGTGCGAAAGGTGACGCGCAGCAGATC
	GCGCGTAAATTCCTGTCTCCGCTGGCGGACAAAGACGCGGTTGGTGGTCTGGGTATCGCG
	AAAGCGGGTAACAAACCGCGTTGGGTTCGTATGCGTGAAGCGGGTGAACCGGGTTGGGAA
	GAAGAAAAAGAAAAAGCGGAAACCCGTAAATCTGCGGACCGTACCGCGGACGTTCTGCGT
	GCGCTGGCGGACTTCGGTCTGAAACCGCTGATGCGTGTTTACACCGACTCTGAAATGTCT
	TCTGTTGAATGGAAACCGCTGCGTAAAGGTCAGGCGGTTCGTACCTGGGACCGTGACATG
	TTCCAGCAGGCGATCGAACGTATGATGTCTTGGGAATCTTGGAACCAGCGTGTTGGTCAG
	GAATACGCGAAACTGGTTGAACAGAAAAACCGTTTCGAACAGAAAAACTTCGTTGGTCAG
	GAACACCTGGTTCACCTGGTTAACCAGCTGCAGCAGGACATGAAAGAAGCGTCTCCGGGT
	CTGGAATCTAAAGAACAGACCGCGCACTACGTTACCGGTCGTGCGCTGCGTGGTTCTGAC
	AAAGTTTTCGAAAAATGGGGTAAACTGGCGCCGGACGCGCCGTTCGACCTGTACGACGCG
	GAAATCAAAAACGTTCAGCGTCGTAACACCCGTCGTTTCGGTTCTCACGACCTGTTCGCG
	AAACTGGCGGAACCGGAATACCAGGCGCTGTGGCGTGAAGACGCGTCTTTCCTGACCCGT
	TACGCGGTTTACAACTCTATCCTGCGTAAACTGAACCACGCGAAAATGTTCGCGACCTTC
	ACCCTGCCGGACGCGACCGCGCACCCGATCTGGACCCGTTTCGACAAACTGGGTGGTAAC
	CTGCACCAGTACACCTTCCTGTTCAACGAATTCGGTGAACGTCGTCACGCGATCCGTTTC
	CACAAACTGCTGAAAGTTGAAAACGGTGTTGCGCGTGAAGTTGACGACGTTACCGTTCCG
	ATCTCTATGTCTGAACAGCTGGACAACCTGCTGCCGCGTGACCCGAACGAACCGATCGCG
	CTGTACTTCCGTGACTACGGTGCGGAACAGCACTTCACCGGTGAATTCGGTGGTGCGAAA
	ATCCAGTGCCGTCGTGACCAGCTGGCGCACATGCACCGTCGTCGTGGTGCGCGTGACGTT
	TACCTGAACGTTTCTGTTCGTGTTCAGTCTCAGTCTGAAGCGCGTGGTGAACGTCGTCCG
	CCGTACGCGGCGGTTTTCCGTCTGGTTGGTGACAACCACCGTGCGTTCGTTCACTTCGAC
	AAACTGTCTGACTACCTGGCGGAACACCCGGACGACGGTAAACTGGGTTCTGAAGGTCTG
	CTGTCTGGTCTGCGTGTTATGTCTGTTGACCTGGGTCTGCGTACCTCTGCGTCTATCTCT
	GTTTTCCGTGTTGCGCGTAAAGACGAACTGAAACCGAACTCTAAAGGTCGTGTTCCGTTC
	TTCTTCCCGATCAAAGGTAACGACAACCTGGTTGCGGTTCACGAACGTTCTCAGCTGCTG
	AAACTGCCGGGTGAAACCGAATCTAAAGACCTGCGTGCGATCCGTGAAGAACGTCAGCGT
	ACCCTGCGTCAGCTGCGTACCCAGCTGGCGTACCTGCGTCTGCTGGTTCGTTGCGGTTCT
	GAAGACGTTGGTCGTCGTGAACGTTCTTGGGCGAAACTGATCGAACAGCCGGTTGACGCG
	GCGAACCACATGACCCCGGACTGGCGTGAAGCGTTCGAAAACGAACTGCAGAAACTGAAA
	TCTCTGCACGGTATCTGCTCTGACAAAGAATGGATGGACGCGGTTTACGAATCTGTTCGT
	CGTGTTTGGCGTCACATGGGTAAACAGGTTCGTGACTGGCGTAAAGACGTTCGTTCTGGT
	GAACGTCCGAAAATCCGTGGTTACGCGAAAGACGTTGTTGGTGGTAACTCTATCGAACAG
	ATCGAATACCTGGAACGTCAGTACAAATTCCTGAAATCTTGGTCTTTCTTCGGTAAAGTT
	TCTGGTCAGGTTATCCGTGCGGAAAAAGGTTCTCGTTTCGCGATCACCCTGCGTGAACAC
	ATCGACCACGCGAAAGAAGACCGTCTGAAAAAACTGGCGGACCGTATCATCATGGAAGCG
	CTGGGTTACGTTTACGCGCTGGACGAACGTGGTAAAGGTAAATGGGTTGCGAAATACCCG
	CCGTGCCAGCTGATCCTGCTGGAAGAACTGTCTGAATACCAGTTCAACAACGACCGTCCG
	CCGTCTGAAAACAACCAGCTGATGCAGTGGTCTCACCGTGGTGTTTTCCAGGAACTGATC
	AACCAGGCGCAGGTTCACGACCTGCTGGTTGGTACCATGTACGCGGCGTTCTCTTCTCGT
	TTCGACGCGCGTACCGGTGCGCCGGGTATCCGTTGCCGTCGTGTTCCGGCGCGTTGCACC
	CAGGAACACAACCCGGAACCGTTCCCGTGGTGGCTGAACAAATTCGTTGTTGAACACACC
	CTGGACGCGTGCCCGCTGCGTGCGGACGACCTGATCCCGACCGGTGAAGGTGAAATCTTC
	GTTTCTCCGTTCTCTGCGGAAGAAGGTGACTTCCACCAGATCCACGCGGACCTGAACGCG
	GCGCAGAACCTGCAGCAGCGTCTGTGGTCTGACTTCGACATCTCTCAGATCCGTCTGCGT
	TGCGACTGGGGTGAAGTTGACGGTGAACTGGTTCTGATCCCGCGTCTGACCGGTAAACGT
	ACCGCGGACTCTTACTCTAACAAAGTTTTCTACACCAACACCGGTGTTACCTACTACGAA
	CGTGAACGTGGTAAAAAACGTCGTAAAGTTTTCGCGCAGGAAAAACTGTCTGAAGAAGAA
	GCGGAACTGCTGGTTGAAGCGGACGAAGCGCGTGAAAAATCTGTTGTTCTGATGCGTGAC
	CCGTCTGGTATCATCAACCGTGGTAACTGGACCCGTCAGAAAGAATTCTGGTCTATGGTT
	AACCAGCGTATCGAAGGTTACCTGGTTAAACAGATCCGTTCTCGTGTTCCGCTGCAGGAC
	TCTGCGTGCGAAAACACCGGTGACATCTAA

SEQ	ATGGCGACCCGTTCTTTCATCCTGAAAATCGAACCGAACGAAGAAGTTAAAAAAGGTCTG
ID	TGGAAAACCCACGAAGTTCTGAACCACGGTATCGCGTACTACATGAACATCCTGAAACTG
NO:	ATCCGTCAGGAAGCGATCTACGAACACCACGAACAGGACCCGAAAAACCCGAAAAAAGTT
54	TCTAAAGCGGAAATCCAGGCGGAACTGTGGGACTTCGTTCTGAAAATGCAGAAATGCAAC
	TCTTTCACCCACGAAGTTGACAAAGACGTTGTTTTCAACATCCTGCGTGAACTGTACGAA
	GAACTGGTTCCGTCTTCTGTTGAAAAAAAAGGTGAAGCGAACCAGCTGTCTAACAAATTC
	CTGTACCCGCTGGTTGACCCGAACTCTCAGTCTGGTAAAGGTACCGCGTCTTCTGGTCGT
	AAACCGCGTTGGTACAACCTGAAAATCGCGGGTGACCCGTCTTGGGAAGAAGAAAAAAAA
	AAATGGGAAGAAGACAAAAAAAAAGACCCGCTGGCGAAAATCCTGGGTAAACTGGCGGAA
	TACGGTCTGATCCCGCTGTTCATCCCGTTCACCGACTCTAACGAACCGATCGTTAAAGAA
	ATCAAATGGATGGAAAAATCTCGTAACCAGTCTGTTCGTCGTCTGGACAAAGACATGTTC
	ATCCAGGCGCTGGAACGTTTCCTGTCTTGGGAATCTTGGAACCTGAAAGTTAAAGAAGAA
	TACGAAAAAGTTGAAAAAGAACACAAAACCCTGGAAGAACGTATCAAAGAAGACATCCAG
	GCGTTCAAATCTCTGGAACAGTACGAAAAAGAACGTCAGGAACAGCTGCTGCGTGACACC
	CTGAACACCAACGAATACCGTCTGTCTAAACGTGGTCTGCGTGGTTGGCGTGAAATCATC
	CAGAAATGGCTGAAAATGGACGAAAACGAACCGTCTGAAAAATACCTGGAAGTTTTCAAA
	GACTACCAGCGTAAACACCCGCGTGAAGCGGGTGACTACTCTGTTTACGAATTCCTGTCT
	AAAAAAGAAAACCACTTCATCTGGCGTAACCACCCGGAATACCCGTACCTGTACGCGACC
	TTCTGCGAAATCGACAAAAAAAAAAAAGACGCGAAACAGCAGGCGACCTTCACCCTGGCG
	GACCCGATCAACCACCCGCTGTGGGTTCGTTTCGAAGAACGTTCTGGTTCTAACCTGAAC
	AAATACCGTATCCTGACCGAACAGCTGCACACCGAAAAACTGAAAAAAAAACTGACCGTT
	CAGCTGGACCGTCTGATCTACCCGACCGAATCTGGTGGTTGGGAAGAAAAAGGTAAAGTT
	GACATCGTTCTGCTGCCGTCTCGTCAGTTCTACAACCAGATCTTCCTGGACATCGAAGAA
	AAAGGTAAACACGCGTTCACCTACAAAGACGAATCTATCAAATTCCCGCTGAAAGGTACC
	CTGGGTGGTGCGCGTGTTCAGTTCGACCGTGACCACCTGCGTCGTTACCCGCACAAAGTT
	GAATCTGGTAACGTTGGTCGTATCTACTTCAACATGACCGTTAACATCGAACCGACCGAA
	TCTCCGGTTTCTAAATCTCTGAAAATCCACCGTGACGACTTCCCGAAATTCGTTAACTTC
	AAACCGAAAGAACTGACCGAATGGATCAAAGACTCTAAAGGTAAAAAACTGAAATCTGGT
	ATCGAATCTCTGGAAATCGGTCTGCGTGTTATGTCTATCGACCTGGGTCAGCGTCAGGCG
	GCGGCGGCGTCTATCTTCGAAGTTGTTGACCAGAAACCGGACATCGAAGGTAAACTGTTC
	TTCCCGATCAAAGGTACCGAACTGTACGCGGTTCACCGTGCGTCTTTCAACATCAAACTG
	CCGGGTGAAACCCTGGTTAAATCTCGTGAAGTTCTGCGTAAAGCGCGTGAAGACAACCTG
	AAACTGATGAACCAGAAACTGAACTTCCTGCGTAACGTTCTGCACTTCCAGCAGTTCGAA
	GACATCACCGAACGTGAAAAACGTGTTACCAAATGGATCTCTCGTCAGGAAAACTCTGAC
	GTTCCGCTGGTTTACCAGGACGAACTGATCCAGATCCGTGAACTGATGTACAAACCGTAC
	AAAGACTGGGTTGCGTTCCTGAAACAGCTGCACAAACGTCTGGAAGTTGAAATCGGTAAA
	GAAGTTAAACACTGGCGTAAATCTCTGTCTGACGGTCGTAAAGGTCTGTACGGTATCTCT
	CTGAAAAACATCGACGAAATCGACCGTACCCGTAAATTCCTGCTGCGTTGGTCTCTGCGT
	CCGACCGAACCGGGTGAAGTTCGTCGTCTGGAACCGGGTCAGCGTTTCGCGATCGACCAG
	CTGAACCACCTGAACGCGCTGAAAGAAGACCGTCTGAAAAAAATGGCGAACACCATCATC
	ATGCACGCGCTGGGTTACTGCTACGACGTTCGTAAAAAAAAATGGCAGGCGAAAAACCCG
	GCGTGCCAGATCATCCTGTTCGAAGACCTGTCTAACTACAACCCGTACGAAGAACGTTCT
	CGTTTCGAAAACTCTAAACTGATGAAATGGTCTCGTCGTGAAATCCCGCGTCAGGTTGCG
	CTGCAGGGTGAAATCTACGGTCTGCAGGTTGGTGAAGTTGGTGCGCAGTTCTCTTCTCGT
	TTCCACGCGAAAACCGGTTCTCCGGGTATCCGTTGCTCTGTTGTTACCAAAGAAAAACTG
	CAGGACAACCGTTTCTTCAAAAACCTGCAGCGTGAAGGTCGTCTGACCCTGGACAAAATC
	GCGGTTCTGAAAGAAGGTGACCTGTACCCGGACAAAGGTGGTGAAAAATTCATCTCTCTG
	TCTAAAGACCGTAAACTGGTTACCACCCACGCGGACATCAACGCGGCGCAGAACCTGCAG
	AAACGTTTCTGGACCCGTACCCACGGTTTCTACAAAGTTTACTGCAAAGCGTACCAGGTT
	GACGGTCAGACCGTTTACATCCCGGAATCTAAAGACCAGAAACAGAAAATCATCGAAGAA
	TTCGGTGAAGGTTACTTCATCCTGAAAGACGGTGTTTACGAATGGGGTAACGCGGGTAAA
	CTGAAAATCAAAAAAGGTTCTTCTAAACAGTCTTCTTCTGAACTGGTTGACTCTGACATC
	CTGAAAGACTCTTTCGACCTGGCGTCTGAACTGAAAGGTGAAAAACTGATGCTGTACCGT
	GACCCGTCTGGTAACGTTTTCCCGTCTGACAAATGGATGGCGGCGGGTGTTTTCTTCGGT
	AAACTGGAACGTATCCTGATCTCTAAACTGACCAACCAGTACTCTATCTCTACCATCGAA
	GACGACTCTTCTAAACAGTCTATGTAA

SEQ	ATGCCGACCCGTACCATCAACCTGAAACTGGTTCTGGGTAAAAACCCGGAAAACGCGACC
ID	CTGCGTCGTGCGCTGTTCTCTACCCACCGTCTGGTTAACCAGGCGACCAAACGTATCGAA
NO:	GAATTCCTGCTGCTGTGCCGTGGTGAAGCGTACCGTACCGTTGACAACGAAGGTAAAGAA
55	GCGGAAATCCCGCGTCACGCGGTTCAGGAAGAAGCGCTGGCGTTCGCGAAAGCGGCGCAG
	CGTCACAACGGTTGCATCTCTACCTACGAAGACCAGGAAATCCTGGACGTTCTGCGTCAG
	CTGTACGAACGTCTGGTTCCGTCTGTTAACGAAAACAACGAAGCGGGTGACGCGCAGGCG
	GCGAACGCGTGGGTTTCTCCGCTGATGTCTGCGGAATCTGAAGGTGGTCTGTCTGTTTAC
	GACAAAGTTCTGGACCCGCCGCCGGTTTGGATGAAACTGAAAGAAGAAAAAGCGCCGGGT
	TGGGAAGCGGCGTCTCAGATCTGGATCCAGTCTGACGAAGGTCAGTCTCTGCTGAACAAA
	CCGGGTTCTCCGCCGCGTTGGATCCGTAAACTGCGTTCTGGTCAGCCGTGGCAGGACGAC
	TTCGTTTCTGACCAGAAAAAAAAACAGGACGAACTGACCAAAGGTAACGCGCCGCTGATC
	AAACAGCTGAAAGAAATGGGTCTGCTGCCGCTGGTTAACCCGTTCTTCCGTCACCTGCTG
	GACCCGGAAGGTAAAGGTGTTTCTCCGTGGGACCGTCTGGCGGTTCGTGCGGCGGTTGCG
	CACTTCATCTCTTGGGAATCTTGGAACCACCGTACCCGTGCGGAATACAACTCTCTGAAA
	CTGCGTCGTGACGAATTCGAAGCGGCGTCTGACGAATTCAAAGACGACTTCACCCTGCTG
	CGTCAGTACGAAGCGAAACGTCACTCTACCCTGAAATCTATCGCGCTGGCGGACGACTCT
	AACCCGTACCGTATCGGTGTTCGTTCTCTGCGTGCGTGGAACCGTGTTCGTGAAGAATGG
	ATCGACAAAGGTGCGACCGAAGAACAGCGTGTTACCATCCTGTCTAAACTGCAGACCCAG
	CTGCGTGGTAAATTCGGTGACCCGGACCTGTTCAACTGGCTGGCGCAGGACCGTCACGTT
	CACCTGTGGTCTCCGCGTGACTCTGTTACCCCGCTGGTTCGTATCAACGCGGTTGACAAA
	GTTCTGCGTCGTCGTAAACCGTACGCGCTGATGACCTTCGCGCACCCGCGTTTCCACCCG
	CGTTGGATCCTGTACGAAGCGCCGGGTGGTTCTAACCTGCGTCAGTACGCGCTGGACTGC
	ACCGAAAACGCGCTGCACATCACCCTGCCGCTGCTGGTTGACGACGCGCACGGTACCTGG
	ATCGAAAAAAAAATCCGTGTTCCGCTGGCGCCGTCTGGTCAGATCCAGGACCTGACCCTG
	GAAAAACTGGAAAAAAAAAAAAACCGTCTGTACTACCGTTCTGGTTTCCAGCAGTTCGCG
	GGTCTGGCGGGTGGTGCGGAAGTTCTGTTCCACCGTCCGTACATGGAACACGACGAACGT
	TCTGAAGAATCTCTGCTGGAACGTCCGGGTGCGGTTTGGTTCAAACTGACCCTGGACGTT
	GCGACCCAGGCGCCGCCGAACTGGCTGGACGGTAAAGGTCGTGTTCGTACCCCGCCGGAA
	GTTCACCACTTCAAAACCGCGCTGTCTAACAAATCTAAACACACCCGTACCCTGCAGCCG
	GGTCTGCGTGTTCTGTCTGTTGACCTGGGTATGCGTACCTTCGCGTCTTGCTCTGTTTTC
	GAACTGATCGAAGGTAAACCGGAAACCGGTCGTGCGTTCCCGGTTGCGGACGAACGTTCT
	ATGGACTCTCCGAACAAACTGTGGGCGAAACACGAACGTTCTTTCAAACTGACCCTGCCG
	GGTGAAACCCCGTCTCGTAAAGAAGAAGAAGAACGTTCTATCGCGCGTGCGGAAATCTAC
	GCGCTGAAACGTGACATCCAGCGTCTGAAATCTCTGCTGCGTCTGGGTGAAGAAGACAAC
	GACAACCGTCGTGACGCGCTGCTGGAACAGTTCTTCAAAGGTTGGGGTGAAGAAGACGTT
	GTTCCGGGTCAGGCGTTCCCGCGTTCTCTGTTCCAGGGTCTGGGTGCGGCGCCGTTCCGT
	TCTACCCCGGAACTGTGGCGTCAGCACTGCCAGACCTACTACGACAAAGCGGAAGCGTGC
	CTGGCGAAACACATCTCTGACTGGCGTAAACGTACCCGTCCGCGTCCGACCTCTCGTGAA
	ATGTGGTACAAAACCCGTTCTTACCACGGTGGTAAATCTATCTGGATGCTGGAATACCTG
	GACGCGGTTCGTAAACTGCTGCTGTCTTGGTCTCTGCGTGGTCGTACCTACGGTGCGATC
	AACCGTCAGGACACCGCGCGTTTCGGTTCTCTGGCGTCTCGTCTGCTGCACCACATCAAC
	TCTCTGAAAGAAGACCGTATCAAAACCGGTGCGGACTCTATCGTTCAGGCGGCGCGTGGT
	TACATCCCGCTGCCGCACGGTAAAGGTTGGGAACAGCGTTACGAACCGTGCCAGCTGATC
	CTGTTCGAAGACCTGGCGCGTTACCGTTTCCGTGTTGACCGTCCGCGTCGTGAAAACTCT
	CAGCTGATGCAGTGGAACCACCGTGCGATCGTTGCGGAAACCACCATGCAGGCGGAACTG
	TACGGTCAGATCGTTGAAAACACCGCGGCGGGTTTCTCTTCTCGTTTCCACGCGGCGACC
	GGTGCGCCGGGTGTTCGTTGCCGTTTCCTGCTGGAACGTGACTTCGACAACGACCTGCCG
	AAACCGTACCTGCTGCGTGAACTGTCTTGGATGCTGGGTAACACCAAAGTTGAATCTGAA
	GAAGAAAAACTGCGTCTGCTGTCTGAAAAAATCCGTCCGGGTTCTCTGGTTCCGTGGGAC
	GGTGGTGAACAGTTCGCGACCCTGCACCCGAAACGTCAGACCCTGTGCGTTATCCACGCG
	GACATGAACGCGGCGCAGAACCTGCAGCGTCGTTTCTTCGGTCGTTGCGGTGAAGCGTTC
	CGTCTGGTTTGCCAGCCGCACGGTGACGACGTTCTGCGTCTGGCGTCTACCCCGGGTGCG
	CGTCTGCTGGGTGCGCTGCAGCAGCTGGAAAACGGTCAGGGTGCGTTCGAACTGGTTCGT
	GACATGGGTTCTACCTCTCAGATGAACCGTTTCGTTATGAAATCTCTGGGTAAAAAAAAA
	ATCAAACCGCTGCAGGACAACAACGGTGACGACGAACTGGAAGACGTTCTGTCTGTTCTG
	CCGGAAGAAGACGACACCGGTCGTATCACCGTTTTCCGTGACTCTTCTGGTATCTTCTTC
	CCGTGCAACGTTTGGATCCCGGCGAAACAGTTCTGGCCGGCGGTTCGTGCGATGATCTGG
	AAAGTTATGGCGTCTCACTCTCTGGGTTAA

SEQ	ATGACCAAACTGCGTCACCGTCAGAAAAAACTGACCCACGACTGGGCGGGTTCTAAAAAA
ID	CGTGAAGTTCTGGGTTCTAACGGTAAACTGCAGAACCCGCTGCTGATGCCGGTTAAAAAA
NO:	GGTCAGGTTACCGAATTCCGTAAAGCGTTCTCTGCGTACGCGCGTGCGACCAAAGGTGAA
56	ATGACCGACGGTCGTAAAAACATGTTCACCCACTCTTTCGAACCGTTCAAAACCAAACCG
	TCTCTGCACCAGTGCGAACTGGCGGACAAAGCGTACCAGTCTCTGCACTCTTACCTGCCG
	GGTTCTCTGGCGCACTTCCTGCTGTCTGCGCACGCGCTGGGTTTCCGTATCTTCTCTAAA
	TCTGGTGAAGCGACCGCGTTCCAGGCGTCTTCTAAAATCGAAGCGTACGAATCTAAACTG
	GCGTCTGAACTGGCGTGCGTTGACCTGTCTATCCAGAACCTGACCATCTCTACCCTGTTC
	AACGCGCTGACCACCTCTGTTCGTGGTAAAGGTGAAGAAACCTCTGCGGACCCGCTGATC
	GCGCGTTTCTACACCCTGCTGACCGGTAAACCGCTGTCTCGTGACACCCAGGGTCCGGAA
	CGTGACCTGGCGGAAGTTATCTCTCGTAAAATCGCGTCTTCTTTCGGTACCTGGAAAGAA
	ATGACCGCGAACCCGCTGCAGTCTCTGCAGTTCTTCGAAGAAGAACTGCACGCGCTGGAC
	GCGAACGTTTCTCTGTCTCCGGCGTTCGACGTTCTGATCAAAATGAACGACCTGCAGGGT
	GACCTGAAAAACCGTACCATCGTTTTCGACCCGGACGCGCCGGTTTTCGAATACAACGCG
	GAAGACCCGGCGGACATCATCATCAAACTGACCGCGCGTTACGCGAAAGAAGCGGTTATC
	AAAAACCAGAACGTTGGTAACTACGTTAAAAACGCGATCACCACCACCAACGCGAACGGT
	CTGGGTTGGCTGCTGAACAAAGGTCTGTCTCTGCTGCCGGTTTCTACCGACGACGAACTG
	CTGGAATTCATCGGTGTTGAACGTTCTCACCCGTCTTGCCACGCGCTGATCGAACTGATC
	GCGCAGCTGGAAGCGCCGGAACTGTTCGAAAAAAACGTTTTCTCTGACACCCGTTCTGAA
	GTTCAGGGTATGATCGACTCTGCGGTTTCTAACCACATCGCGCGTCTGTCTTCTTCTCGT
	AACTCTCTGTCTATGGACTCTGAAGAACTGGAACGTCTGATCAAATCTTTCCAGATCCAC
	ACCCCGCACTGCTCTCTGTTCATCGGTGCGCAGTCTCTGTCTCAGCAGCTGGAATCTCTG
	CCGGAAGCGCTGCAGTCTGGTGTTAACTCTGCGGACATCCTGCTGGGTTCTACCCAGTAC
	ATGCTGACCAACTCTCTGGTTGAAGAATCTATCGCGACCTACCAGCGTACCCTGAACCGT
	ATCAACTACCTGTCTGGTGTTGCGGGTCAGATCAACGGTGCGATCAAACGTAAAGCGATC
	GACGGTGAAAAAATCCACCTGCCGGCGGCGTGGTCTGAACTGATCTCTCTGCCGTTCATC
	GGTCAGCCGGTTATCGACGTTGAATCTGACCTGGCGCACCTGAAAAACCAGTACCAGACC
	CTGTCTAACGAATTCGACACCCTGATCTCTGCGCTGCAGAAAAACTTCGACCTGAACTTC
	AACAAAGCGCTGCTGAACCGTACCCAGCACTTCGAAGCGATGTGCCGTTCTACCAAAAAA
	AACGCGCTGTCTAAACCGGAAATCGTTTCTTACCGTGACCTGCTGGCGCGTCTGACCTCT
	TGCCTGTACCGTGGTTCTCTGGTTCTGCGTCGTGCGGGTATCGAAGTTCTGAAAAAACAC
	AAAATCTTCGAATCTAACTCTGAACTGCGTGAACACGTTCACGAACGTAAACACTTCGTT
	TTCGTTTCTCCGCTGGACCGTAAAGCGAAAAAACTGCTGCGTCTGACCGACTCTCGTCCG
	GACCTGCTGCACGTTATCGACGAAATCCTGCAGCACGACAACCTGGAAAACAAAGACCGT
	GAATCTCTGTGGCTGGTTCGTTCTGGTTACCTGCTGGCGGGTCTGCCGGACCAGCTGTCT
	TCTTCTTTCATCAACCTGCCGATCATCACCCAGAAAGGTGACCGTCGTCTGATCGACCTG
	ATCCAGTACGACCAGATCAACCGTGACGCGTTCGTTATGCTGGTTACCTCTGCGTTCAAA
	TCTAACCTGTCTGGTCTGCAGTACCGTGCGAACAAACAGTCTTTCGTTGTTACCCGTACC
	CTGTCTCCGTACCTGGGTTCTAAACTGGTTTACGTTCCGAAAGACAAAGACTGGCTGGTT
	CCGTCTCAGATGTTCGAAGGTCGTTTCGCGGACATCCTGCAGTCTGACTACATGGTTTGG
	AAAGACGCGGGTCGTCTGTGCGTTATCGACACCGCGAAACACCTGTCTAACATCAAAAAA
	TCTGTTTTCTCTTCTGAAGAAGTTCTGGCGTTCCTGCGTGAACTGCCGCACCGTACCTTC
	ATCCAGACCGAAGTTCGTGGTCTGGGTGTTAACGTTGACGGTATCGCGTTCAACAACGGT
	GACATCCCGTCTCTGAAAACCTTCTCTAACTGCGTTCAGGTTAAAGTTTCTCGTACCAAC
	ACCTCTCTGGTTCAGACCCTGAACCGTTGGTTCGAAGGTGGTAAAGTTTCTCCGCCGTCT
	ATCCAGTTCGAACGTGCGTACTACAAAAAAGACGACCAGATCCACGAAGACGCGGCGAAA
	CGTAAAATCCGTTTCCAGATGCCGGCGACCGAACTGGTTCACGCGTCTGACGACGCGGGT
	TGGACCCCGTCTTACCTGCTGGGTATCGACCCGGGTGAATACGGTATGGGTCTGTCTCTG
	GTTTCTATCAACAACGGTGAAGTTCTGGACTCTGGTTTCATCCACATCAACTCTCTGATC
	AACTTCGCGTCTAAAAAATCTAACCACCAGACCAAAGTTGTTCCGCGTCAGCAGTACAAA
	TCTCCGTACGCGAACTACCTGGAACAGTCTAAAGACTCTGCGGCGGGTGACATCGCGCAC
	ATCCTGGACCGTCTGATCTACAAACTGAACGCGCTGCCGGTTTTCGAAGCGCTGTCTGGT
	AACTCTCAGTCTGCGGCGGACCAGGTTTGGACCAAAGTTCTGTCTTTCTACACCTGGGGT
	GACAACGACGCGCAGAACTCTATCCGTAAACAGCACTGGTTCGGTGCGTCTCACTGGGAC
	ATCAAAGGTATGCTGCGTCAGCCGCCGACCGAAAAAAAACCGAAACCGTACATCGCGTTC
	CCGGGTTCTCAGGTTTCTTCTTACGGTAACTCTCAGCGTTGCTCTTGCTGCGGTCGTAAC
	CCGATCGAACAGCTGCGTGAAATGGCGAAAGACACCTCTATCAAAGAACTGAAAATCCGT
	AACTCTGAAATCCAGCTGTTCGACGGTACCATCAAACTGTTCAACCCGGACCCGTCTACC
	GTTATCGAACGTCGTCGTCACAACCTGGGTCCGTCTCGTATCCCGGTTGCGGACCGTACC
	TTCAAAAACATCTCTCCGTCTTCTCTGGAATTCAAAGAACTGATCACCATCGTTTCTCGT
	TCTATCCGTCACTCTCCGGAATTCATCGCGAAAAAACGTGGTATCGGTTCTGAATACTTC
	TGCGCGTACTCTGACTGCAACTCTTCTCTGAACTCTGAAGCGAACGCGGCGGCGAACGTT
	GCGCAGAAATTCCAGAAACAGCTGTTCTTCGAACTGTAA

SEQ	ATGAAACGTATCCTGAACTCTCTGAAAGTTGCGGCGCTGCGTCTGCTGTTCCGTGGTAAA
ID	GGTTCTGAACTGGTTAAAACCGTTAAATACCCGCTGGTTTCTCCGGTTCAGGGTGCGGTT
NO:	GAAGAACTGGCGGAAGCGATCCGTCACGACAACCTGCACCTGTTCGGTCAGAAAGAAATC
57	GTTGACCTGATGGAAAAAGACGAAGGTACCCAGGTTTACTCTGTTGTTGACTTCTGGCTG
	GACACCCTGCGTCTGGGTATGTTCTTCTCTCCGTCTGCGAACGCGCTGAAAATCACCCTG
	GGTAAATTCAACTCTGACCAGGTTTCTCCGTTCCGTAAAGTTCTGGAACAGTCTCCGTTC
	TTCCTGGCGGGTCGTCTGAAAGTTGAACCGGCGGAACGTATCCTGTCTGTTGAAATCCGT
	AAAATCGGTAAACGTGAAAACCGTGTTGAAAACTACGCGGCGGACGTTGAAACCTGCTTC
	ATCGGTCAGCTGTCTTCTGACGAAAAACAGTCTATCCAGAAACTGGCGAACGACATCTGG
	GACTCTAAAGACCACGAAGAACAGCGTATGCTGAAAGCGGACTTCTTCGCGATCCCGCTG
	ATCAAAGACCCGAAAGCGGTTACCGAAGAAGACCCGGAAAACGAAACCGCGGGTAAACAG
	AAACCGCTGGAACTGTGCGTTTGCCTGGTTCCGGAACTGTACACCCGTGGTTTCGGTTCT
	ATCGCGGACTTCCTGGTTCAGCGTCTGACCCTGCTGCGTGACAAAATGTCTACCGACACC
	GCGGAAGACTGCCTGGAATACGTTGGTATCGAAGAAGAAAAAGGTAACGGTATGAACTCT
	CTGCTGGGTACCTTCCTGAAAAACCTGCAGGGTGACGGTTTCGAACAGATCTTCCAGTTC
	ATGCTGGGTTCTTACGTTGGTTGGCAGGGTAAAGAAGACGTTCTGCGTGAACGTCTGGAC
	CTGCTGGCGGAAAAAGTTAAACGTCTGCCGAAACCGAAATTCGCGGGTGAATGGTCTGGT
	CACCGTATGTTCCTGCACGGTCAGCTGAAATCTTGGTCTTCTAACTTCTTCCGTCTGTTC
	AACGAAACCCGTGAACTGCTGGAATCTATCAAATCTGACATCCAGCACGCGACCATGCTG
	ATCTCTTACGTTGAAGAAAAAGGTGGTTACCACCCGCAGCTGCTGTCTCAGTACCGTAAA
	CTGATGGAACAGCTGCCGGCGCTGCGTACCAAAGTTCTGGACCCGGAAATCGAAATGACC
	CACATGTCTGAAGCGGTTCGTTCTTACATCATGATCCACAAATCTGTTGCGGGTTTCCTG
	CCGGACCTGCTGGAATCTCTGGACCGTGACAAAGACCGTGAATTCCTGCTGTCTATCTTC
	CCGCGTATCCCGAAAATCGACAAAAAAACCAAAGAAATCGTTGCGTGGGAACTGCCGGGT
	GAACCGGAAGAAGGTTACCTGTTCACCGCGAACAACCTGTTCCGTAACTTCCTGGAAAAC
	CCGAAACACGTTCCGCGTTTCATGGCGGAACGTATCCCGGAAGACTGGACCCGTCTGCGT
	TCTGCGCCGGTTTGGTTCGACGGTATGGTTAAACAGTGGCAGAAAGTTGTTAACCAGCTG
	GTTGAATCTCCGGGTGCGCTGTACCAGTTCAACGAATCTTTCCTGCGTCAGCGTCTGCAG
	GCGATGCTGACCGTTTACAAACGTGACCTGCAGACCGAAAAATTCCTGAAACTGCTGGCG
	GACGTTTGCCGTCCGCTGGTTGACTTCTTCGGTCTGGGTGGTAACGACATCATCTTCAAA
	TCTTGCCAGGACCCGCGTAAACAGTGGCAGACCGTTATCCCGCTGTCTGTTCCGGCGGAC
	GTTTACACCGCGTGCGAAGGTCTGGCGATCCGTCTGCGTGAAACCCTGGGTTTCGAATGG
	AAAAACCTGAAAGGTCACGAACGTGAAGACTTCCTGCGTCTGCACCAGCTGCTGGGTAAC
	CTGCTGTTCTGGATCCGTGACGCGAAACTGGTTGTTAAACTGGAAGACTGGATGAACAAC
	CCGTGCGTTCAGGAATACGTTGAAGCGCGTAAAGCGATCGACCTGCCGCTGGAAATCTTC
	GGTTTCGAAGTTCCGATCTTCCTGAACGGTTACCTGTTCTCTGAACTGCGTCAGCTGGAA
	CTGCTGCTGCGTCGTAAATCTGTTATGACCTCTTACTCTGTTAAAACCACCGGTTCTCCG
	AACCGTCTGTTCCAGCTGGTTTACCTGCCGCTGAACCCGTCTGACCCGGAAAAAAAAAAC
	TCTAACAACTTCCAGGAACGTCTGGACACCCCGACCGGTCTGTCTCGTCGTTTCCTGGAC
	CTGACCCTGGACGCGTTCGCGGGTAAACTGCTGACCGACCCGGTTACCCAGGAACTGAAA
	ACCATGGCGGGTTTCTACGACCACCTGTTCGGTTTCAAACTGCCGTGCAAACTGGCGGCG
	ATGTCTAACCACCCGGGTTCTTCTTCTAAAATGGTTGTTCTGGCGAAACCGAAAAAAGGT
	GTTGCGTCTAACATCGGTTTCGAACCGATCCCGGACCCGGCGCACCCGGTTTTCCGTGTT
	CGTTCTTCTTGGCCGGAACTGAAATACCTGGAAGGTCTGCTGTACCTGCCGGAAGACACC
	CCGCTGACCATCGAACTGGCGGAAACCTCTGTTTCTTGCCAGTCTGTTTCTTCTGTTGCG
	TTCGACCTGAAAAACCTGACCACCATCCTGGGTCGTGTTGGTGAATTCCGTGTTACCGCG
	GACCAGCCGTTCAAACTGACCCCGATCATCCCGGAAAAAGAAGAATCTTTCATCGGTAAA
	ACCTACCTGGGTCTGGACGCGGGTGAACGTTCTGGTGTTGGTTTCGCGATCGTTACCGTT
	GACGGTGACGGTTACGAAGTTCAGCGTCTGGGTGTTCACGAAGACACCCAGCTGATGGCG
	CTGCAGCAGGTTGCGTCTAAATCTCTGAAAGAACCGGTTTTCCAGCCGCTGCGTAAAGGT
	ACCTTCCGTCAGCAGGAACGTATCCGTAAATCTCTGCGTGGTTGCTACTGGAACTTCTAC
	CACGCGCTGATGATCAAATACCGTGCGAAAGTTGTTCACGAAGAATCTGTTGGTTCTTCT
	GGTCTGGTTGGTCAGTGGCTGCGTGCGTTCCAGAAAGACCTGAAAAAAGCGGACGTTCTG
	CCGAAAAAAGGTGGTAAAAACGGTGTTGACAAAAAAAAACGTGAATCTTCTGCGCAGGAC
	ACCCTGTGGGGTGGTGCGTTCTCTAAAAAAGAAGAACAGCAGATCGCGTTCGAAGTTCAG
	GCGGCGGGTTCTTCTCAGTTCTGCCTGAAATGCGGTTGGTGGTTCCAGCTGGGTATGCGT
	GAAGTTAACCGTGTTCAGGAATCTGGTGTTGTTCTGGACTGGAACCGTTCTATCGTTACC
	TTCCTGATCGAATCTTCTGGTGAAAAAGTTTACGGTTTCTCTCCGCAGCAGCTGGAAAAA
	GGTTTCCGTCCGGACATCGAAACCTTCAAAAAAATGGTTCGTGACTTCATGCGTCCGCCG
	ATGTTCGACCGTAAAGGTCGTCCGGCGGCGGCGTACGAACGTTTCGTTCTGGGTCGTCGT
	CACCGTCGTTACCGTTTCGACAAAGTTTTCGAAGAACGTTTCGGTCGTTCTGCGCTGTTC
	ATCTGCCCGCGTGTTGGTTGCGGTAACTTCGACCACTCTTCTGAACAGTCTGCGGTTGTT
	CTGGCGCTGATCGGTTACATCGCGGACAAAGAAGGTATGTCTGGTAAAAAACTGGTTTAC
	GTTCGTCTGGCGGAACTGATGGCGGAATGGAAACTGAAAAAACTGGAACGTTCTCGTGTT
	GAAGAACAGTCTTCTGCGCAGTAA

SEQ	ATGGCGGAATCTAAACAGATGCAGTGCCGTAAATGCGGTGCGTCTATGAAATACGAAGTT
ID	ATCGGTCTGGGTAAAAAATCTTGCCGTTACATGTGCCCGGACTGCGGTAACCACACCTCT
NO:	GCGCGTAAAATCCAGAACAAAAAAAAACGTGACAAAAAATACGGTTCTGCGTCTAAAGCG
58	CAGTCTCAGCGTATCGCGGTTGCGGGTGCGCTGTACCCGGACAAAAAAGTTCAGACCATC
	AAAACCTACAAATACCCGGCGGACCTGAACGGTGAAGTTCACGACTCTGGTGTTGCGGAA
	AAAATCGCGCAGGCGATCCAGGAAGACGAAATCGGTCTGCTGGGTCCGTCTTCTGAATAC
	GCGTGCTGGATCGCGTCTCAGAAACAGTCTGAACCGTACTCTGTTGTTGACTTCTGGTTC
	GACGCGGTTTGCGCGGGTGGTGTTTTCGCGTACTCTGGTGCGCGTCTGCTGTCTACCGTT
	CTGCAGCTGTCTGGTGAAGAATCTGTTCTGCGTGCGGCGCTGGCGTCTTCTCCGTTCGTT
	GACGACATCAACCTGGCGCAGGCGGAAAAATTCCTGGCGGTTTCTCGTCGTACCGGTCAG
	GACAAACTGGGTAAACGTATCGGTGAATGCTTCGCGGAAGGTCGTCTGGAAGCGCTGGGT
	ATCAAAGACCGTATGCGTGAATTCGTTCAGGCGATCGACGTTGCGCAGACCGCGGGTCAG
	CGTTTCGCGGCGAAACTGAAAATCTTCGGTATCTCTCAGATGCCGGAAGCGAAACAGTGG
	AACAACGACTCTGGTCTGACCGTTTGCATCCTGCCGGACTACTACGTTCCGGAAGAAAAC
	CGTGCGGACCAGCTGGTTGTTCTGCTGCGTCGTCTGCGTGAAATCGCGTACTGCATGGGT
	ATCGAAGACGAAGCGGGTTTCGAACACCTGGGTATCGACCCGGGTGCGCTGTCTAACTTC
	TCTAACGGTAACCCGAAACGTGGTTTCCTGGGTCGTCTGCTGAACAACGACATCATCGCG
	CTGGCGAACAACATGTCTGCGATGACCCCGTACTGGGAAGGTCGTAAAGGTGAACTGATC
	GAACGTCTGGCGTGGCTGAAACACCGTGCGGAAGGTCTGTACCTGAAAGAACCGCACTTC
	GGTAACTCTTGGGCGGACCACCGTTCTCGTATCTTCTCTCGTATCGCGGGTTGGCTGTCT
	GGTTGCGCGGGTAAACTGAAAATCGCGAAAGACCAGATCTCTGGTGTTCGTACCGACCTG
	TTCCTGCTGAAACGTCTGCTGGACGCGGTTCCGCAGTCTGCGCCGTCTCCGGACTTCATC
	GCGTCTATCTCTGCGCTGGACCGTTTCCTGGAAGCGGCGGAATCTTCTCAGGACCCGGCG
	GAACAGGTTCGTGCGCTGTACGCGTTCCACCTGAACGCGCCGGCGGTTCGTTCTATCGCG
	AACAAAGCGGTTCAGCGTTCTGACTCTCAGGAATGGCTGATCAAAGAACTGGACGCGGTT
	GACCACCTGGAATTCAACAAAGCGTTCCCGTTCTTCTCTGACACCGGTAAAAAAAAAAAA
	AAAGGTGCGAACTCTAACGGTGCGCCGTCTGAAGAAGAATACACCGAAACCGAATCTATC
	CAGCAGCCGGAAGACGCGGAACAGGAAGTTAACGGTCAGGAAGGTAACGGTGCGTCTAAA
	AACCAGAAAAAATTCCAGCGTATCCCGCGTTTCTTCGGTGAAGGTTCTCGTTCTGAATAC
	CGTATCCTGACCGAAGCGCCGCAGTACTTCGACATGTTCTGCAACAACATGCGTGCGATC
	TTCATGCAGCTGGAATCTCAGCCGCGTAAAGCGCCGCGTGACTTCAAATGCTTCCTGCAG
	AACCGTCTGCAGAAACTGTACAAACAGACCTTCCTGAACGCGCGTTCTAACAAATGCCGT
	GCGCTGCTGGAATCTGTTCTGATCTCTTGGGGTGAATTCTACACCTACGGTGCGAACGAA
	AAAAAATTCCGTCTGCGTCACGAAGCGTCTGAACGTTCTTCTGACCCGGACTACGTTGTT
	CAGCAGGCGCTGGAAATCGCGCGTCGTCTGTTCCTGTTCGGTTTCGAATGGCGTGACTGC
	TCTGCGGGTGAACGTGTTGACCTGGTTGAAATCCACAAAAAAGCGATCTCTTTCCTGCTG
	GCGATCACCCAGGCGGAAGTTTCTGTTGGTTCTTACAACTGGCTGGGTAACTCTACCGTT
	TCTCGTTACCTGTCTGTTGCGGGTACCGACACCCTGTACGGTACCCAGCTGGAAGAATTC
	CTGAACGCGACCGTTCTGTCTCAGATGCGTGGTCTGGCGATCCGTCTGTCTTCTCAGGAA
	CTGAAAGACGGTTTCGACGTTCAGCTGGAATCTTCTTGCCAGGACAACCTGCAGCACCTG
	CTGGTTTACCGTGCGTCTCGTGACCTGGCGGCGTGCAAACGTGCGACCTGCCCGGCGGAA
	CTGGACCCGAAAATCCTGGTTCTGCCGGTTGGTGCGTTCATCGCGTCTGTTATGAAAATG
	ATCGAACGTGGTGACGAACCGCTGGCGGGTGCGTACCTGCGTCACCGTCCGCACTCTTTC
	GGTTGGCAGATCCGTGTTCGTGGTGTTGCGGAAGTTGGTATGGACCAGGGTACCGCGCTG
	GCGTTCCAGAAACCGACCGAATCTGAACCGTTCAAAATCAAACCGTTCTCTGCGCAGTAC
	GGTCCGGTTCTGTGGCTGAACTCTTCTTCTTACTCTCAGTCTCAGTACCTGGACGGTTTC
	CTGTCTCAGCCGAAAAACTGGTCTATGCGTGTTCTGCCGCAGGCGGGTTCTGTTCGTGTT
	GAACAGCGTGTTGCGCTGATCTGGAACCTGCAGGCGGGTAAAATGCGTCTGGAACGTTCT
	GGTGCGCGTGCGTTCTTCATGCCGGTTCCGTTCTCTTTCCGTCCGTCTGGTTCTGGTGAC
	GAAGCGGTTCTGGCGCCGAACCGTTACCTGGGTCTGTTCCCGCACTCTGGTGGTATCGAA
	TACGCGGTTGTTGACGTTCTGGACTCTGCGGGTTTCAAAATCCTGGAACGTGGTACCATC
	GCGGTTAACGGTTTCTCTCAGAAACGTGGTGAACGTCAGGAAGAAGCGCACCGTGAAAAA
	CAGCGTCGTGGTATCTCTGACATCGGTCGTAAAAAACCGGTTCAGGCGGAAGTTGACGCG
	GCGAACGAACTGCACCGTAAATACACCGACGTTGCGACCCGTCTGGGTTGCCGTATCGTT
	GTTCAGTGGGCGCCGCAGCCGAAACCGGGTACCGCGCCGACCGCGCAGACCGTTTACGCG
	CGTGCGGTTCGTACCGAAGCGCCGCGTTCTGGTAACCAGGAAGACCACGCGCGTATGAAA
	TCTTCTTGGGGTTACACCTGGGGTACCTACTGGGAAAAACGTAAACCGGAAGACATCCTG
	GGTATCTCTACCCAGGTTTACTGGACCGGTGGTATCGGTGAATCTTGCCCGGCGGTTGCG
	GTTGCGCTGCTGGGTCACATCCGTGCGACCTCTACCCAGACCGAATGGGAAAAAGAAGAA
	GTTGTTTTCGGTCGTCTGAAAAAATTCTTCCCGTCTTAA

SEQ	ATGGAAAAACGTATCAACAAAATCCGTAAAAAACTGTCTGCGGACAACGCGACCAAACCG
ID	GTTTCTCGTTCTGGTCCGATGAAAACCCTGCTGGTTCGTGTTATGACCGACGACCTGAAA
NO:	AAACGTCTGGAAAAACGTCGTAAAAAACCGGAAGTTATGCCGCAGGTTATCTCTAACAAC
59	GCGGCGAACAACCTGCGTATGCTGCTGGACGACTACACCAAAATGAAAGAAGCGATCCTG
	CAGGTTTACTGGCAGGAATTCAAAGACGACCACGTTGGTCTGATGTGCAAATTCGCGCAG
	CCGGCGTCTAAAAAAATCGACCAGAACAAACTGAAACCGGAAATGGACGAAAAAGGTAAC
	CTGACCACCGCGGGTTTCGCGTGCTCTCAGTGCGGTCAGCCGCTGTTCGTTTACAAACTG
	GAACAGGTTTCTGAAAAAGGTAAAGCGTACACCAACTACTTCGGTCGTTGCAACGTTGCG
	GAACACGAAAAACTGATCCTGCTGGCGCAGCTGAAACCGGAAAAAGACTCTGACGAAGCG
	GTTACCTACTCTCTGGGTAAATTCGGTCAGCGTGCGCTGGACTTCTACTCTATCCACGTT
	ACCAAAGAATCTACCCACCCGGTTAAACCGCTGGCGCAGATCGCGGGTAACCGTTACGCG
	TCTGGTCCGGTTGGTAAAGCGCTGTCTGACGCGTGCATGGGTACCATCGCGTCTTTCCTG
	TCTAAATACCAGGACATCATCATCGAACACCAGAAAGTTGTTAAAGGTAACCAGAAACGT
	CTGGAATCTCTGCGTGAACTGGCGGGTAAAGAAAACCTGGAATACCCGTCTGTTACCCTG
	CCGCCGCAGCCGCACACCAAAGAAGGTGTTGACGCGTACAACGAAGTTATCGCGCGTGTT
	CGTATGTGGGTTAACCTGAACCTGTGGCAGAAACTGAAACTGTCTCGTGACGACGCGAAA
	CCGCTGCTGCGTCTGAAAGGTTTCCCGTCTTTCCCGGTTGTTGAACGTCGTGAAAACGAA
	GTTGACTGGTGGAACACCATCAACGAAGTTAAAAAACTGATCGACGCGAAACGTGACATG
	GGTCGTGTTTTCTGGTCTGGTGTTACCGCGGAAAAACGTAACACCATCCTGGAAGGTTAC
	AACTACCTGCCGAACGAAAACGACCACAAAAAACGTGAAGGTTCTCTGGAAAACCCGAAA
	AAACCGGCGAAACGTCAGTTCGGTGACCTGCTGCTGTACCTGGAAAAAAAATACGCGGGT
	GACTGGGGTAAAGTTTTCGACGAAGCGTGGGAACGTATCGACAAAAAAATCGCGGGTCTG
	ACCTCTCACATCGAACGTGAAGAAGCGCGTAACGCGGAAGACGCGCAGTCTAAAGCGGTT
	CTGACCGACTGGCTGCGTGCGAAAGCGTCTTTCGTTCTGGAACGTCTGAAAGAAATGGAC
	GAAAAAGAATTCTACGCGTGCGAAATCCAGCTGCAGAAATGGTACGGTGACCTGCGTGGT
	AACCCGTTCGCGGTTGAAGCGGAAAACCGTGTTGTTGACATCTCTGGTTTCTCTATCGGT
	TCTGACGGTCACTCTATCCAGTACCGTAACCTGCTGGCGTGGAAATACCTGGAAAACGGT
	AAACGTGAATTCTACCTGCTGATGAACTACGGTAAAAAAGGTCGTATCCGTTTCACCGAC
	GGTACCGACATCAAAAAATCTGGTAAATGGCAGGGTCTGCTGTACGGTGGTGGTAAAGCG
	AAAGTTATCGACCTGACCTTCGACCCGGACGACGAACAGCTGATCATCCTGCCGCTGGCG
	TTCGGTACCCGTCAGGGTCGTGAATTCATCTGGAACGACCTGCTGTCTCTGGAAACCGGT
	CTGATCAAACTGGCGAACGGTCGTGTTATCGAAAAAACCATCTACAACAAAAAAATCGGT
	CGTGACGAACCGGCGCTGTTCGTTGCGCTGACCTTCGAACGTCGTGAAGTTGTTGACCCG
	TCTAACATCAAACCGGTTAACCTGATCGGTGTTGACCGTGGTGAAAACATCCCGGCGGTT
	ATCGCGCTGACCGACCCGGAAGGTTGCCCGCTGCCGGAATTCAAAGACTCTTCTGGTGGT
	CCGACCGACATCCTGCGTATCGGTGAAGGTTACAAAGAAAAACAGCGTGCGATCCAGGCG
	GCGAAAGAAGTTGAACAGCGTCGTGCGGGTGGTTACTCTCGTAAATTCGCGTCTAAATCT
	CGTAACCTGGCGGACGACATGGTTCGTAACTCTGCGCGTGACCTGTTCTACCACGCGGTT
	ACCCACGACGCGGTTCTGGTTTTCGAAAACCTGTCTCGTGGTTTCGGTCGTCAGGGTAAA
	CGTACCTTCATGACCGAACGTCAGTACACCAAAATGGAAGACTGGCTGACCGCGAAACTG
	GCGTACGAAGGTCTGACCTCTAAAACCTACCTGTCTAAAACCCTGGCGCAGTACACCTCT
	AAAACCTGCTCTAACTGCGGTTTCACCATCACCACCGCGGACTACGACGGTATGCTGGTT
	CGTCTGAAAAAAACCTCTGACGGTTGGGCGACCACCCTGAACAACAAAGAACTGAAAGCG
	GAAGGTCAGATCACCTACTACAACCGTTACAAACGTCAGACCGTTGAAAAAGAACTGTCT
	GCGGAACTGGACCGTCTGTCTGAAGAATCTGGTAACAACGACATCTCTAAATGGACCAAA
	GGTCGTCGTGACGAAGCGCTGTTCCTGCTGAAAAAACGTTTCTCTCACCGTCCGGTTCAG
	GAACAGTTCGTTTGCCTGGACTGCGGTCACGAAGTTCACGCGGACGAACAGGCGGCGCTG
	AACATCGCGCGTTCTTGGCTGTTCCTGAACTCTAACTCTACCGAATTCAAATCTTACAAA
	TCTGGTAAACAGCCGTTCGTTGGTGCGTGGCAGGCGTTCTACAAACGTCGTCTGAAAGAA
	GTTTGGAAACCGAACGCG

SEQ	ATGAAACGTATCAACAAAATCCGTCGTCGTCTGGTTAAAGACTCTAACACCAAAAAAGCG
ID	GGTAAAACCGGTCCGATGAAAACCCTGCTGGTTCGTGTTATGACCCCGGACCTGCGTGAA
NO:	CGTCTGGAAAACCTGCGTAAAAAACCGGAAAACATCCCGCAGCCGATCTCTAACACCTCT
60	CGTGCGAACCTGAACAAACTGCTGACCGACTACACCGAAATGAAAAAAGCGATCCTGCAC
	GTTTACTGGGAAGAATTCCAGAAAGACCCGGTTGGTCTGATGTCTCGTGTTGCGCAGCCG
	GCGCCGAAAAACATCGACCAGCGTAAACTGATCCCGGTTAAAGACGGTAACGAACGTCTG
	ACCTCTTCTGGTTTCGCGTGCTCTCAGTGCTGCCAGCCGCTGTACGTTTACAAACTGGAA
	CAGGTTAACGACAAAGGTAAACCGCACACCAACTACTTCGGTCGTTGCAACGTTTCTGAA
	CACGAACGTCTGATCCTGCTGTCTCCGCACAAACCGGAAGCGAACGACGAACTGGTTACC
	TACTCTCTGGGTAAATTCGGTCAGCGTGCGCTGGACTTCTACTCTATCCACGTTACCCGT
	GAATCTAACCACCCGGTTAAACCGCTGGAACAGATCGGTGGTAACTCTTGCGCGTCTGGT
	CCGGTTGGTAAAGCGCTGTCTGACGCGTGCATGGGTGCGGTTGCGTCTTTCCTGACCAAA
	TACCAGGACATCATCCTGGAACACCAGAAAGTTATCAAAAAAAACGAAAAACGTCTGGCG
	AACCTGAAAGACATCGCGTCTGCGAACGGTCTGGCGTTCCCGAAAATCACCCTGCCGCCG
	CAGCCGCACACCAAAGAAGGTATCGAAGCGTACAACAACGTTGTTGCGCAGATCGTTATC
	TGGGTTAACCTGAACCTGTGGCAGAAACTGAAAATCGGTCGTGACGAAGCGAAACCGCTG
	CAGCGTCTGAAAGGTTTCCCGTCTTTCCCGCTGGTTGAACGTCAGGCGAACGAAGTTGAC
	TGGTGGGACATGGTTTGCAACGTTAAAAAACTGATCAACGAAAAAAAAGAAGACGGTAAA
	GTTTTCTGGCAGAACCTGGCGGGTTACAAACGTCAGGAAGCGCTGCTGCCGTACCTGTCT
	TCTGAAGAAGACCGTAAAAAAGGTAAAAAATTCGCGCGTTACCAGTTCGGTGACCTGCTG
	CTGCACCTGGAAAAAAAACACGGTGAAGACTGGGGTAAAGTTTACGACGAAGCGTGGGAA
	CGTATCGACAAAAAAGTTGAAGGTCTGTCTAAACACATCAAACTGGAAGAAGAACGTCGT
	TCTGAAGACGCGCAGTCTAAAGCGGCGCTGACCGACTGGCTGCGTGCGAAAGCGTCTTTC
	GTTATCGAAGGTCTGAAAGAAGCGGACAAAGACGAATTCTGCCGTTGCGAACTGAAACTG
	CAGAAATGGTACGGTGACCTGCGTGGTAAACCGTTCGCGATCGAAGCGGAAAACTCTATC
	CTGGACATCTCTGGTTTCTCTAAACAGTACAACTGCGCGTTCATCTGGCAGAAAGACGGT
	GTTAAAAAACTGAACCTGTACCTGATCATCAACTACTTCAAAGGTGGTAAACTGCGTTTC
	AAAAAAATCAAACCGGAAGCGTTCGAAGCGAACCGTTTCTACACCGTTATCAACAAAAAA
	TCTGGTGAAATCGTTCCGATGGAAGTTAACTTCAACTTCGACGACCCGAACCTGATCATC
	CTGCCGCTGGCGTTCGGTAAACGTCAGGGTCGTGAATTCATCTGGAACGACCTGCTGTCT
	CTGGAAACCGGTTCTCTGAAACTGGCGAACGGTCGTGTTATCGAAAAAACCCTGTACAAC
	CGTCGTACCCGTCAGGACGAACCGGCGCTGTTCGTTGCGCTGACCTTCGAACGTCGTGAA
	GTTCTGGACTCTTCTAACATCAAACCGATGAACCTGATCGGTATCGACCGTGGTGAAAAC
	ATCCCGGCGGTTATCGCGCTGACCGACCCGGAAGGTTGCCCGCTGTCTCGTTTCAAAGAC
	TCTCTGGGTAACCCGACCCACATCCTGCGTATCGGTGAATCTTACAAAGAAAAACAGCGT
	ACCATCCAGGCGGCGAAAGAAGTTGAACAGCGTCGTGCGGGTGGTTACTCTCGTAAATAC
	GCGTCTAAAGCGAAAAACCTGGCGGACGACATGGTTCGTAACACCGCGCGTGACCTGCTG
	TACTACGCGGTTACCCAGGACGCGATGCTGATCTTCGAAAACCTGTCTCGTGGTTTCGGT
	CGTCAGGGTAAACGTACCTTCATGGCGGAACGTCAGTACACCCGTATGGAAGACTGGCTG
	ACCGCGAAACTGGCGTACGAAGGTCTGCCGTCTAAAACCTACCTGTCTAAAACCCTGGCG
	CAGTACACCTCTAAAACCTGCTCTAACTGCGGTTTCACCATCACCTCTGCGGACTACGAC
	CGTGTTCTGGAAAAACTGAAAAAAACCGCGACCGGTTGGATGACCACCATCAACGGTAAA
	GAACTGAAAGTTGAAGGTCAGATCACCTACTACAACCGTTACAAACGTCAGAACGTTGTT
	AAAGACCTGTCTGTTGAACTGGACCGTCTGTCTGAAGAATCTGTTAACAACGACATCTCT
	TCTTGGACCAAAGGTCGTTCTGGTGAAGCGCTGTCTCTGCTGAAAAAACGTTTCTCTCAC
	CGTCCGGTTCAGGAAAAATTCGTTTGCCTGAACTGCGGTTTCGAAACCCACGCGGACGAA
	CAGGCGGCGCTGAACATCGCGCGTTCTTGGCTGTTCCTGCGTTCTCAGGAATACAAAAAA
	TACCAGACCAACAAAACCACCGGTAACACCGACAAACGTGCGTTCGTTGAAACCTGGCAG
	TCTTTCTACCGTAAAAAACTGAAAGAAGTTTGGAAACCG

SEQ	AAAATTCcatGCAAAATGCTCCGGTTTCATGTCATCAAAATGATGACGTAATTAAGCATT
ID	GATAATTGAGATCCCTCTCCCTGACAGGATGATTACATAAATAATAGTGACAAAAATAAA
NO:	TTATTTATTTATCCAGAAAATGAATTGGAAAATCAGGAGAGCGTTTTCAATCCTACCTCT
61	GGCGCAGTTGATATGTcaaaCAGGTtgccgtcactgcgtcttttactggctcttctcgct
	aaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccat
	gacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
	gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctac
	ctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcac
	cgcctatctcgtgtgagataggcggagatacgaactttaagAAGGAGatataccATGGGT
	AAAATGTATTACCTTGGTTTAGACATTGGCACGAATTCCGTGGGCTACGCGGTGACCGAC
	CCCTCATACCACCTGCTGAAGTTTAAGGGGGAACCAATGTGGGGTGCGCACGTATTTGCC
	GCCGGTAATCAGAGCGCGGAACGACGCTCGTTCCGCACATCGCGTCGTCGTTTGGACCGA
	CGCCAACAGCGCGTTAAACTGGTACAGGAGATTTTTGCCCCGGTGATTAGTCCGATCGAC
	CCACGCTTCTTCATTCGTCTGCATGAATCCGCCCTGTGGCGCGATGACGTCGCGGAGACG
	GATAAACATATCTTTTTCAATGATCCTACCTATACCGATAAGGAATATTATAGCGATTAC
	CCGACTATCCATCACCTGATCGTTGATCTGATGGAAAGCTCTGAGAAACACGATCCGCGG
	CTGGTGTACCTTGCAGTGGCGTGGTTAGTGGCACACCGTGGTCATTTTCTGAACGAGGTG
	GACAAGGATAATATTGGAGATGTGTTGTCGTTCGACGCATTTTATCCGGAGTTTCTCGCG
	TTCCTGTCGGACAACGGTGTATCACCGTGGGTGTGCGAAAGCAAAGCGCTGCAGGCGACC
	TTGCTGAGCCGTAACTCAGTGAACGACAAATATAAAGCCCTTAAGTCTCTGATCTTCGGA
	TCCCAGAAACCTGAAGATAACTTCGATGCCAATATTTCGGAAGATGGACTCATTCAACTG
	CTGGCCGGCAAAAAGGTAAAAGTTAACAAACTGTTCCCTCAGGAATCGAACGATGCATCC
	TTCACATTGAATGATAAAGAAGACGCGATAGAAGAAATCCTGGGTACGCTTACACCAGAT
	GAATGTGAATGGATTGCGCATATACGCCGCCTTTTTGACTGGGCTATCATGAAACATGCT
	CTGAAAGATGGCAGGACTATTAGCGAGTCAAAAGTCAAACTGTATGAGCAGCACCATCAC
	GATCTGACCCAACTTAAATACTTCGTGAAAACCTACCTTGCAAAAGAATACGACGATATT
	TTCCGCAACGTGGATAGCGAAACAACGAAAAACTATGTAGCGTATTCCTATCATGTGAAA
	GAGGTGAAAGGCACTCTGCCTAAAAATAAGGCAACGCAAGAAGAGTTTTGTAAGTATGTC
	CTGGGCAAGGTTAAAAACATTGAATGCTCTGAAGCAGACAAGGTTGACTTTGATGAGATG
	ATTCAGCGTCTTACCGACAACTCTTTTATGCCTAAGCAGGTTTCGGGCGAAAACCGCGTT
	ATTCCTTATCAGTTATATTATTATGAACTGAAGACAATTCTGAATAAAGCAGCCTCGTAC
	CTGCCTTTCCTGACGCAGTGTGGAAAAGATGCAATTTCGAACCAGGACAAACTACTGTCG
	ATCATGACGTTCCGTATTCCTTACTTCGTCGGACCCTTGCGAAAAGATAATTCGGAACAT
	GCATGGCTCGAACGAAAGGCCGGTAAGATTTATCCGTGGAACTTTAACGACAAAGTGGAC
	TTGGATAAATCAGAAGAAGCGTTCATTCGCCGAATGACCAATACCTGTACCTATTATCCC
	GGCGAAGATGTTTTACCGTTGGATTCGCTGATCTATGAGAAATTTATGATTTTAAATGAA
	ATCAATAATATTCGTATTGACGGCTACCCGATTAGTGTTGACGTTAAACAGCAGGTTTTT
	GGCTTGTTCGAAAAAAAACGACGCGTAACCGTGAAAGATATTCAGAACCTGCTGCTGTCT
	CTCGGAGCTCTGGACAAACACGGGAAGCTGACAGGCATCGATACCACTATCCACTCAAAC
	TATAATACGTATCACCATTTTAAATCTCTCATGGAACGCGGCGTCCTGACCCGGGATGAC
	GTGGAACGCATCGTTGAAAGGATGACCTACAGCGACGATACTAAGCGTGTGCGTCTGTGG
	CTGAATAACAACTATGGTACTTTAACCGCCGACGATGTGAAACACATTTCGCGTCTGCGC
	AAACACGATTTTGGCCGTTTATCCAAAATGTTCTTAACAGGTCTGAAGGGTGTCCATAAG
	GAGACCGGTGAACGTGCCTCCATACTGGATTTCATGTGGAACACGAACGATAACCTGATG
	CAGCTCCTTTCCGAATGCTACACGTTCAGTGATGAAATCACAAAGCTGCAAGAGGCGTAT
	TATGCAAAAGCCCAGTTGTCTTTAAACGATTTTTTAGACTCGATGTACATCTCTAACGCG
	GTGAAACGTCCGATTTACAGAACTCTGGCAGTGGTGAACGATATTCGAAAAGCATGTGGG
	ACGGCCCCTAAACGCATTTTCATCGAAATGGCTCGTGATGGTGAATCAAAAAAAAAGAGA
	AGTGTTACACGTCGCGAGCAGATCAAAAACCTGTACCGCTCGATTCGTAAAGATTTCCAG
	CAGGAAGTTGATTTTCTGGAAAAGATCCTGGAAAATAAATCTGATGGTCAACTTCAGTCA
	GATGCTTTGTATCTTTACTTTGCACAATTAGGGCGCGATATGTACACGGGCGATCCAATA
	AAGCTGGAGCACATCAAAGATCAGAGTTTCTATAACATAGACCATATTTACCCGCAGTCT
	ATGGTGAAAGACGATTCCCTAGATAACAAAGTGCTGGTGCAAAGCGAAATTAACGGCGAG
	AAAAGCTCGCGATACCCTTTGGACGCCGCGATCCGCAATAAAATGAAGCCCCTTTGGGAC
	GCTTACTATAATCATGGCCTGATCTCCTTAAAGAAATACCAGCGTCTAACGCGCTCGACC
	CCGTTTACCGATGATGAAAAATGGGACTTTATTAATCGCCAGTTAGTGGAAACCCGTCAA
	TCTACCAAAGCGCTGGCCATTTTGTTGAAGCGTAAGTTTCCAGACACCGAAATTGTGTAT
	TCGAAGGCGGGGTTATCGTCCGACTTCAGACATGAATTCGGCCTTGTAAAAAGTCGCAAT
	ATTAATGATTTGCACCACGCTAAAGACGCATTCTTGGCTATCGTTACCGGCAATGTGTAC
	CATGAAAGATTCAATCGCAGATGGTTTATGGTGAACCAGCCGTACTCAGTTAAAACTAAA
	ACTCTTTTTACCCACAGCATAAAGAATGGCAACTTCGTTGCCTGGAACGGCGAAGAAGAT
	CTCGGTCGTATTGTAAAAATGCTGAAGCAAAACAAAAATACCATTCACTTCACGCGCTTC
	TCCTTCGATCGCAAAGAAGGATTATTTGATATCCAACCTCTGAAAGCCAGCACCGGCTTA
	GTCCCACGAAAAGCCGGTCTGGATGTCGTTAAATACGGCGGATATGACAAATCTACCGCG
	GCCTATTACCTGCTGGTGAGGTTCACGCTCGAGGACAAGAAAACCCAGCACAAGCTGATG
	ATGATTCCTGTAGAAGGCCTGTACAAGGCTCGCATTGATCATGACAAGGAATTTCTTACC
	GATTATGCGCAAACGACTATAAGCGAAATCCTACAGAAAGATAAACAGAAAGTGATCAAT
	ATTATGTTTCCAATGGGTACGAGGCATATAAAACTCAATTCAATGATTAGTATCGATGGC
	TTCTATCTTAGTATCGGCGGAAAGTCCTCTAAAGGTAAGTCAGTTCTATGTCACGCAATG
	GTTCCACTGATCGTCCCTCACAAAATCGAATGTTACATTAAAGCAATGGAAAGCTTCGCC
	CGGAAGTTTAAAGAAAACAACAAGCTGCGCATCGTAGAAAAATTCGATAAAATCACCGTT
	GAAGACAACCTGAATCTCTACGAGCTCTTTCTCCAAAAACTGCAGCATAATCCCTATAAT
	AAGTTTTTTTCGACACAGTTTGACGTACTGACGAACGGCCGTTCTACTTTCACAAAACTG
	TCGCCGGAGGAACAGGTACAGACGCTCTTGAACATTTTAAGTATCTTTAAAACATGCCGC
	AGTTCGGGTTGCGACCTGAAATCCATCAACGGCAGTGCCCAGGCAGCGCGCATCATGATT
	AGCGCTGACTTAACTGGACTGTCGAAAAAATATTCAGATATTAGGTTGGTTGAACAGTCA
	GCTTCTGGTTTGTTCGTATCCAAAAGTCAGAACTTACTGGAGTATCTCTAAGAAATCATC
	CTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTTATTATATCGCGTTGATTATTGAT
	GCTGTTTTTAGTTTTAACGGCAATTAATATATGTGTTATTAATTGAATGAATTTTATCAT
	TCATAATAAGTATGTGTAGGATCAAGCTCAGGTTAAATATTCACTCAGGAAGTTATTACT
	CAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAGTGTTAAGGGATGTTATTTCC

SEQ	AAAATTCcatGCAAAATGCTCCGGTTTCATGTCATCAAAATGATGACGTAATTAAGCATT
ID	GATAATTGAGATCCCTCTCCCTGACAGGATGATTACATAAATAATAGTGACAAAAATAAA
NO:	TTATTTATTTATCCAGAAAATGAATTGGAAAATCAGGAGAGCGTTTTCAATCCTACCTCT
62	GGCGCAGTTGATATGTcaaaCAGGTtgccgtcactgcgtcttttactggctcttctcgct
	aaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccat
	gacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
	gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctac
	ctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcac
	cgcctatctcgtgtgagataggcggagatacgaactttaagAAGGAGatataccATGTCA
	TCGCTCACGAAATTCACTAACAAATACTCTAAACAGCTCACCATTAAGAATGAACTCATC
	CCAGTTGGCAAAACACTGGAGAACATCAAAGAGAATGGTCTGATAGATGGCGACGAACAG
	CTGAATGAGAATTATCAGAAGGCGAAAATTATTGTGGATGATTTTCTGCGGGACTTCATT
	AATAAAGCACTGAATAATACGCAGATCGGGAACTGGCGCGAACTGGCGGATGCCCTTAAT
	AAAGAGGATGAAGATAACATCGAGAAATTGCAGGATAAAATTCGGGGAATCATTGTATCC
	AAATTTGAAACGTTTGATCTGTTTAGCAGCTATTCTATTAAGAAAGATGAAAAGATTATT
	GACGACGACAATGATGTTGAAGAAGAGGAACTGGATCTGGGCAAGAAGACCAGCTCATTT
	AAATACATATTTAAAAAAAACCTGTTTAAGTTAGTGTTGCCATCCTACCTGAAAACCACA
	AACCAGGACAAGCTGAAGATTATTAGCTCGTTTGATAATTTTTCAACGTACTTCCGCGGG
	TTCTTTGAAAACCGGAAAAACATTTTTACCAAGAAACCGATCTCCACAAGTATTGCGTAT
	CGCATTGTTCATGATAACTTCCCGAAATTCCTTGATAACATTCGTTGTTTTAATGTGTGG
	CAGACGGAATGCCCGCAACTAATCGTGAAAGCAGATAACTATCTGAAAAGCAAAAATGTT
	ATAGCGAAAGATAAAAGTTTGGCAAACTATTTTACCGTGGGCGCGTATGACTATTTCCTG
	TCTCAGAATGGTATAGATTTTTACAACAATATTATAGGTGGACTGCCAGCGTTCGCCGGC
	CATGAGAAAATCCAAGGTCTCAATGAATTCATCAATCAAGAGTGCCAAAAAGACAGCGAG
	CTGAAAAGTAAGCTGAAAAACCGTCACGCGTTCAAAATGGCGGTACTGTTCAAACAGATA
	CTCAGCGATCGTGAAAAAAGTTTTGTAATTGATGAGTTCGAGTCGGATGCTCAAGTTATT
	GACGCCGTTAAAAACTTTTACGCCGAACAGTGCAAAGATAACAATGTTATTTTTAACTTA
	TTAAATCTTATCAAGAATATCGCTTTCTTAAGTGATGACGAACTGGACGGCATATTCATT
	GAAGGGAAATACCTGTCGAGCGTTAGTCAAAAACTCTATAGCGATTGGTCAAAATTACGT
	AACGACATTGAGGATTCGGCTAACTCTAAACAAGGCAATAAAGAGCTGGCCAAGAAGATC
	AAAACCAACAAAGGGGATGTAGAAAAAGCGATCTCGAAATATGAGTTCTCGCTGTCGGAA
	CTGAACTCGATTGTACATGATAACACCAAGTTTTCTGACCTCCTTAGTTGTACACTGCAT
	AAGGTGGCTTCTGAGAAACTGGTGAAGGTCAATGAAGGCGACTGGCCGAAACATCTCAAG
	AATAATGAAGAGAAACAAAAAATCAAAGAGCCGCTTGATGCTCTGCTGGAGATCTATAAT
	ACACTTCTGATTTTTAACTGCAAAAGCTTCAATAAAAACGGCAACTTCTATGTCGACTAT
	GATCGTTGCATCAATGAACTGAGTTCGGTCGTGTATCTGTATAATAAAACACGTAACTAT
	TGCACTAAAAAACCCTATAACACGGACAAGTTCAAACTCAATTTTAACAGTCCGCAGCTC
	GGTGAAGGCTTTTCCAAGTCGAAAGAAAATGACTGTCTGACTCTTTTGTTTAAAAAAGAC
	GACAACTATTATGTAGGCATTATCCGCAAAGGTGCAAAAATCAATTTTGATGATACACAA
	GCAATCGCCGATAACACCGACAATTGCATCTTTAAAATGAATTATTTCCTACTTAAAGAC
	GCAAAAAAATTTATCCCGAAATGTAGCATTCAGCTGAAAGAAGTCAAGGCCCATTTTAAG
	AAATCTGAAGATGATTACATTTTGTCTGATAAAGAGAAATTTGCTAGCCCGCTGGTCATT
	AAAAAGAGCACATTTTTGCTGGCAACTGCACATGTGAAAGGGAAAAAAGGCAATATCAAG
	AAATTTCAGAAAGAATATTCGAAAGAAAACCCCACTGAGTATCGCAATTCTTTAAACGAA
	TGGATTGCTTTTTGTAAAGAGTTCTTAAAAACTTATAAAGCGGCTACCATTTTTGATATA
	ACCACATTGAAAAAGGCAGAGGAATATGCTGATATTGTAGAATTCTACAAGGATGTCGAT
	AATCTGTGCTACAAACTGGAGTTCTGCCCGATTAAAACCTCGTTTATAGAAAACCTGATA
	GATAACGGCGACCTGTATCTGTTTCGCATCAATAACAAAGACTTCAGCAGTAAATCGACC
	GGCACCAAGAACCTTCATACGTTATATTTACAAGCTATATTCGATGAACGTAATCTGAAC
	AATCCGACAATTATGCTGAATGGGGGAGCAGAACTGTTCTATCGTAAAGAAAGTATTGAG
	CAGAAAAACCGTATCACACACAAAGCCGGTTCAATTCTCGTGAATAAGGTGTGTAAAGAC
	GGTACAAGCCTGGATGATAAGATACGTAATGAAATTTATCAATATGAGAATAAATTTATT
	GATACCCTGTCTGATGAAGCTAAAAAGGTGTTACCGAATGTCATTAAAAAGGAAGCTACC
	CATGACATTACAAAAGATAAACGTTTCACTAGTGACAAATTCTTCTTTCACTGCCCCCTG
	ACAATTAATTATAAGGAAGGCGATACCAAGCAGTTCAATAACGAAGTGCTGAGTTTTCTG
	CGTGGAAATCCTGACATCAACATTATCGGCATTGACCGCGGAGAGCGTAATTTAATCTAT
	GTAACGGTTATAAACCAGAAAGGCGAGATTCTGGATTCGGTTTCATTCAATACCGTGACC
	AACAAGAGTTCAAAAATCGAGCAGACAGTCGATTATGAAGAGAAATTGGCAGTCCGCGAG
	AAAGAGAGGATTGAAGCAAAACGTTCCTGGGACTCTATCTCAAAAATTGCGACACTAAAG
	GAAGGTTATCTGAGCGCAATAGTTCACGAGATCTGTCTGTTAATGATTAAACACAACGCG
	ATCGTTGTCTTAGAGAATCTTAATGCAGGCTTTAAGCGTATTCGTGGCGGTTTATCAGAA
	AAAAGTGTTTATCAAAAATTCGAAAAAATGTTGATTAACAAACTGAACTATTTTGTCAGC
	AAGAAGGAATCCGACTGGAATAAACCGTCTGGTCTGCTGAATGGACTGCAGCTTTCGGAT
	CAGTTTGAAAGCTTCGAAAAACTGGGTATTCAGTCTGGTTTTATTTTTTACGTGCCGGCT
	GCATATACCTCAAAGATTGATCCGACCACGGGCTTCGCCAATGTTCTGAATCTGTCGAAG
	GTACGCAATGTTGATGCGATCAAAAGCTTTTTTTCTAACTTCAACGAAATTAGTTATAGC
	AAGAAAGAAGCCCTTTTCAAATTCTCATTCGATCTGGATTCACTGAGTAAGAAAGGCTTT
	AGTAGCTTTGTGAAATTTAGTAAGAGTAAATGGAACGTCTACACCTTTGGAGAACGTATC
	ATAAAGCCAAAGAATAAGCAAGGTTATCGGGAGGACAAAAGAATCAACTTGACCTTCGAG
	ATGAAGAAGTTACTTAACGAGTATAAGGTTTCTTTTGATCTTGAAAATAACTTGATTCCG
	AATCTCACGAGTGCCAACCTGAAGGATACTTTTTGGAAAGAGCTATTCTTTATCTTCAAG
	ACTACGCTGCAGCTCCGTAACAGCGTTACTAACGGTAAAGAAGATGTGCTCATCTCTCCG
	GTCAAAAATGCGAAGGGTGAATTCTTCGTTTCGGGAACGCATAACAAGACTCTTCCGCAA
	GATTGCGATGCGAACGGTGCATACCATATTGCGTTGAAAGGTCTGATGATACTCGAACGT
	AACAACCTTGTACGTGAGGAGAAAGATACGAAAAAGATTATGGCGATTTCAAACGTGGAT
	TGGTTCGAGTACGTGCAGAAACGTAGAGGCGTTCTGTAAGAAATCATCCTTAGCGAAAGC
	TAAGGATTTTTTTTATCTGAAATTTATTATATCGCGTTGATTATTGATGCTGTTTTTAGT
	TTTAACGGCAATTAATATATGTGTTATTAATTGAATGAATTTTATCATTCATAATAAGTA
	TGTGTAGGATCAAGCTCAGGTTAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAG
	AGGATTACAGAATTATCTCATAACAAGTGTTAAGGGATGTTATTTCC

SEQ	AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID	TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO:	CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
63	TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
	ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
	TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
	GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
	ACCATAACAACTACGACGAATTCACCAAACTGTACCCGATCCAGAAAACCATCCGTTTCG
	AACTGAAACCGCAGGGTCGTACCATGGAACACCTGGAAACCTTCAACTTCTTCGAAGAAG
	ACCGTGACCGTGCGGAAAAATACAAAATCCTGAAAGAAGCGATCGACGAATACCACAAAA
	AATTCATCGACGAACACCTGACCAACATGTCTCTGGACTGGAACTCTCTGAAACAGATCT
	CTGAAAAATACTACAAATCTCGTGAAGAAAAAGACAAAAAAGTTTTCCTGTCTGAACAGA
	AACGTATGCGTCAGGAAATCGTTTCTGAATTCAAAAAAGACGACCGTTTCAAAGACCTGT
	TCTCTAAAAAACTGTTCTCTGAACTGCTGAAAGAAGAAATCTACAAAAAAGGTAACCACC
	AGGAAATCGACGCGCTGAAATCTTTCGACAAATTCTCTGGTTACTTCATCGGTCTGCACG
	AAAACCGTAAAAACATGTACTCTGACGGTGACGAAATCACCGCGATCTCTAACCGTATCG
	TTAACGAAAACTTCCCGAAATTCCTGGACAACCTGCAGAAATACCAGGAAGCGCGTAAAA
	AATACCCGGAATGGATCATCAAAGCGGAATCTGCGCTGGTTGCGCACAACATCAAAATGG
	ACGAAGTTTTCTCTCTGGAATACTTCAACAAAGTTCTGAACCAGGAAGGTATCCAGCGTT
	ACAACCTGGCGCTGGGTGGTTACGTTACCAAATCTGGTGAAAAAATGATGGGTCTGAACG
	ACGCGCTGAACCTGGCGCACCAGTCTGAAAAATCTTCTAAAGGTCGTATCCACATGACCC
	CGCTGTTCAAACAGATCCTGTCTGAAAAAGAATCTTTCTCTTACATCCCGGACGTTTTCA
	CCGAAGACTCTCAGCTGCTGCCGTCTATCGGTGGTTTCTTCGCGCAGATCGAAAACGACA
	AAGACGGTAACATCTTCGACCGTGCGCTGGAACTGATCTCTTCTTACGCGGAATACGACA
	CCGAACGTATCTACATCCGTCAGGCGGACATCAACCGTGTTTCTAACGTTATCTTCGGTG
	AATGGGGTACCCTGGGTGGTCTGATGCGTGAATACAAAGCGGACTCTATCAACGACATCA
	ACCTGGAACGTACCTGCAAAAAAGTTGACAAATGGCTGGACTCTAAAGAATTCGCGCTGT
	CTGACGTTCTGGAAGCGATCAAACGTACCGGTAACAACGACGCGTTCAACGAATACATCT
	CTAAAATGCGTACCGCGCGTGAAAAAATCGACGCGGCGCGTAAAGAAATGAAATTCATCT
	CTGAAAAAATCTCTGGTGACGAAGAATCTATCCACATCATCAAAACCCTGCTGGACTCTG
	TTCAGCAGTTCCTGCACTTCTTCAACCTGTTCAAAGCGCGTCAGGACATCCCGCTGGACG
	GTGCGTTCTACGCGGAATTCGACGAAGTTCACTCTAAACTGTTCGCGATCGTTCCGCTGT
	ACAACAAAGTTCGTAACTACCTGACCAAAAACAACCTGAACACCAAAAAAATCAAACTGA
	ACTTCAAAAACCCGACCCTGGCGAACGGTTGGGACCAGAACAAAGTTTACGACTACGCGT
	CTCTGATCTTCCTGCGTGACGGTAACTACTACCTGGGTATCATCAACCCGAAACGTAAAA
	AAAACATCAAATTCGAACAGGGTTCTGGTAACGGTCCGTTCTACCGTAAAATGGTTTACA
	AACAGATCCCGGGTCCGAACAAAAACCTGCCGCGTGTTTTCCTGACCTCTACCAAAGGTA
	AAAAAGAATACAAACCGTCTAAAGAAATCATCGAAGGTTACGAAGCGGACAAACACATCC
	GTGGTGACAAATTCGACCTGGACTTCTGCCACAAACTGATCGACTTCTTCAAAGAATCTA
	TCGAAAAACACAAAGACTGGTCTAAATTCAACTTCTACTTCTCTCCGACCGAATCTTACG
	GTGACATCTCTGAATTCTACCTGGACGTTGAAAAACAGGGTTACCGTATGCACTTCGAAA
	ACATCTCTGCGGAAACCATCGACGAATACGTTGAAAAAGGTGACCTGTTCCTGTTCCAGA
	TCTACAACAAAGACTTCGTTAAAGCGGCGACCGGTAAAAAAGACATGCACACCATCTACT
	GGAACGCGGCGTTCTCTCCGGAAAACCTGCAGGACGTTGTTGTTAAACTGAACGGTGAAG
	CGGAACTGTTCTACCGTGACAAATCTGACATCAAAGAAATCGTTCACCGTGAAGGTGAAA
	TCCTGGTTAACCGTACCTACAACGGTCGTACCCCGGTTCCGGACAAAATCCACAAAAAAC
	TGACCGACTACCACAACGGTCGTACCAAAGACCTGGGTGAAGCGAAAGAATACCTGGACA
	AAGTTCGTTACTTCAAAGCGCACTACGACATCACCAAAGACCGTCGTTACCTGAACGACA
	AAATCTACTTCCACGTTCCGCTGACCCTGAACTTCAAAGCGAACGGTAAAAAAAACCTGA
	ACAAAATGGTTATCGAAAAATTCCTGTCTGACGAAAAAGCGCACATCATCGGTATCGACC
	GTGGTGAACGTAACCTGCTGTACTACTCTATCATCGACCGTTCTGGTAAAATCATCGACC
	AGCAGTCTCTGAACGTTATCGACGGTTTCGACTACCGTGAAAAACTGAACCAGCGTGAAA
	TCGAAATGAAAGACGCGCGTCAGTCTTGGAACGCGATCGGTAAAATCAAAGACCTGAAAG
	AAGGTTACCTGTCTAAAGCGGTTCACGAAATCACCAAAATGGCGATCCAGTACAACGCGA
	TCGTTGTTATGGAAGAACTGAACTACGGTTTCAAACGTGGTCGTTTCAAAGTTGAAAAAC
	AGATCTACCAGAAATTCGAAAACATGCTGATCGACAAAATGAACTACCTGGTTTTCAAAG
	ACGCGCCGGACGAATCTCCGGGTGGTGTTCTGAACGCGTACCAGCTGACCAACCCGCTGG
	AATCTTTCGCGAAACTGGGTAAACAGACCGGTATCCTGTTCTACGTTCCGGCGGCGTACA
	CCTCTAAAATCGACCCGACCACCGGTTTCGTTAACCTGTTCAACACCTCTTCTAAAACCA
	ACGCGCAGGAACGTAAAGAATTCCTGCAGAAATTCGAATCTATCTCTTACTCTGCGAAAG
	ACGGTGGTATCTTCGCGTTCGCGTTCGACTACCGTAAATTCGGTACCTCTAAAACCGACC
	ACAAAAACGTTTGGACCGCGTACACCAACGGTGAACGTATGCGTTACATCAAAGAAAAAA
	AACGTAACGAACTGTTCGACCCGTCTAAAGAAATCAAAGAAGCGCTGACCTCTTCTGGTA
	TCAAATACGACGGTGGTCAGAACATCCTGCCGGACATCCTGCGTTCTAACAACAACGGTC
	TGATCTACACCATGTACTCTTCTTTCATCGCGGCGATCCAGATGCGTGTTTACGACGGTA
	AAGAAGACTACATCATCTCTCCGATCAAAAACTCTAAAGGTGAATTCTTCCGTACCGACC
	CGAAACGTCGTGAACTGCCGATCGACGCGGACGCGAACGGTGCGTACAACATCGCGCTGC
	GTGGTGAACTGACCATGCGTGCGATCGCGGAAAAATTCGACCCGGACTCTGAAAAAATGG
	CGAAACTGGAACTGAAACACAAAGACTGGTTCGAATTCATGCAGACCCGTGGTGACTAAG
	AAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATGTAGGGAGACCCTCAGG
	TTAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACA

SEQ	AAAATTCcatGCAAAATGCTCCGGTTTCATGTCATCAAAATGATGACGTAATTAAGCATT
ID	GATAATTGAGATCCCTCTCCCTGACAGGATGATTACATAAATAATAGTGACAAAAATAAA
NO:	TTATTTATTTATCCAGAAAATGAATTGGAAAATCAGGAGAGCGTTTTCAATCCTACCTCT
64	GGCGCAGTTGATATGTcaaaCAGGTtgccgtcactgcgtcttttactggctcttctcgct
	aaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccat
	gacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
	gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctac
	ctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcac
	cgcctatctcgtgtgagataggcggagatacgaactttaagAAGGAGatataccATGACT
	AAAACATTTGATTCAGAGTTTTTTAATTTGTACTCGCTGCAAAAAACGGTACGCTTTGAG
	TTAAAACCCGTGGGAGAAACCGCGTCATTTGTGGAAGACTTTAAAAACGAGGGCTTGAAA
	CGTGTTGTGAGCGAAGATGAAAGGCGAGCCGTCGATTACCAGAAAGTTAAGGAAATAATT
	GACGATTACCATCGGGATTTCATTGAAGAAAGTTTAAATTATTTTCCGGAACAGGTGAGT
	AAAGATGCTCTTGAGCAGGCGTTTCATCTTTATCAGAAACTGAAGGCAGCAAAAGTTGAG
	GAAAGGGAAAAAGCGCTGAAAGAATGGGAAGCGCTGCAGAAAAAGCTACGTGAAAAAGTG
	GTGAAATGCTTCTCGGACTCGAATAAAGCCCGCTTCTCAAGGATTGATAAAAAGGAACTG
	ATTAAGGAAGACCTGATAAATTGGTTGGTCGCCCAGAATCGCGAGGATGATATCCCTACG
	GTCGAAACGTTTAACAACTTCACCACATATTTTACCGGCTTCCATGAGAATCGTAAAAAT
	ATTTACTCCAAAGATGATCACGCCACCGCTATTAGCTTTCGCCTTATTCATGAAAATCTT
	CCAAAGTTTTTTGACAACGTGATTAGCTTCAATAAGTTGAAAGAGGGTTTCCCTGAATTA
	AAATTTGATAAAGTGAAAGAGGATTTAGAAGTAGATTATGATCTGAAGCATGCGTTTGAA
	ATAGAATATTTCGTTAACTTCGTGACCCAAGCGGGCATAGATCAGTATAATTATCTGTTA
	GGAGGGAAAACCCTGGAGGACGGGACGAAAAAACAAGGGATGAATGAGCAAATTAATCTG
	TTCAAACAACAGCAAACGCGAGATAAAGCGCGTCAGATTCCCAAACTGATCCCCCTGTTC
	AAACAGATTCTTAGCGAAAGGACTGAAAGCCAGTCCTTTATTCCTAAACAATTTGAAAGT
	GATCAGGAGTTGTTCGATTCACTGCAGAAGTTACATAATAACTGCCAGGATAAATTCACC
	GTGCTGCAACAAGCCATTCTCGGTCTGGCAGAGGCGGATCTTAAGAAGGTCTTCATCAAA
	ACCTCTGATTTAAATGCCTTATCTAACACCATTTTCGGGAATTACAGCGTCTTTTCCGAT
	GCACTGAACCTGTATAAAGAAAGCCTGAAAACGAAAAAAGCGCAGGAGGCTTTTGAGAAA
	CTACCGGCCCATTCTATTCACGACCTCATTCAATACTTGGAACAGTTCAATTCCAGCCTG
	GACGCGGAAAAACAACAGAGCACCGACACCGTCCTGAACTACTTCATCAAGACCGATGAA
	TTATATTCTCGCTTCATTAAATCCACTAGCGAGGCTTTCACTCAGGTGCAGCCTTTGTTC
	GAACTGGAAGCCCTGTCATCTAAGCGCCGCCCACCGGAATCGGAAGATGAAGGGGCAAAA
	GGGCAGGAAGGCTTCGAGCAGATCAAGCGTATTAAAGCTTACCTGGATACGCTTATGGAA
	GCGGTACACTTTGCAAAGCCGTTGTATCTTGTTAAGGGTCGTAAAATGATCGAAGGGCTC
	GATAAAGACCAGTCCTTTTATGAAGCGTTTGAAATGGCGTACCAAGAACTTGAATCGTTA
	ATCATTCCTATCTATAACAAAGCGCGGAGCTATCTGTCGCGGAAACCTTTCAAGGCCGAT
	AAATTCAAGATTAATTTTGACAACAACACGCTACTGAGCGGATGGGATGCGAACAAGGAA
	ACTGCTAACGCGTCCATTCTGTTTAAGAAAGACGGGTTATATTACCTTGGAATTATGCCG
	AAAGGTAAGACCTTTCTCTTTGACTACTTTGTATCGAGCGAGGATTCAGAGAAACTGAAA
	CAGCGTCGCCAGAAGACCGCCGAAGAAGCTCTGGCGCAGGATGGTGAAAGTTACTTCGAA
	AAAATTCGTTATAAACTGTTACCAGGGGCTTCAAAGATGTTACCGAAAGTCTTTTTTAGC
	AACAAAAATATTGGCTTTTACAACCCGTCGGATGACATTTTACGCATTCGCAACACAGCC
	TCTCACACCAAAAACGGGACCCCTCAGAAAGGCCACTCAAAAGTTGAGTTTAACCTGAAT
	GATTGTCATAAGATGATTGATTTCTTCAAATCATCAATTCAGAAACACCCGGAATGGGGG
	TCTTTTGGCTTTACGTTTTCTGATACCAGTGATTTTGAAGACATGAGTGCCTTCTACCGG
	GAAGTAGAAAACCAGGGTTACGTAATTAGCTTTGACAAAATCAAAGAGACCTATATACAG
	AGCCAGGTGGAACAGGGTAATCTCTACTTATTCCAGATTTATAACAAGGATTTCTCGCCC
	TACAGCAAAGGCAAACCAAACCTGCATACTCTGTACTGGAAAGCCCTGTTTGAAGAAGCG
	AACCTGAATAACGTAGTGGCGAAGTTGAACGGTGAAGCGGAAATCTTCTTCCGTCGTCAC
	TCCATTAAGGCCTCTGATAAAGTTGTCCATCCGGCAAATCAGGCCATTGATAATAAGAAT
	CCACACACGGAAAAAACGCAGTCAACCTTTGAATATGACCTCGTTAAAGACAAACGCTAC
	ACGCAAGATAAGTTCTTTTTCCACGTCCCAATCAGCCTCAACTTTAAAGCACAAGGGGTT
	TCAAAGTTTAATGATAAAGTCAATGGGTTCCTCAAGGGCAACCCGGATGTCAACATTATA
	GGTATAGACAGGGGCGAACGCCATCTGCTTTACTTTACCGTAGTGAATCAGAAAGGTGAA
	ATACTGGTTCAGGAATCATTAAATACCTTGATGTCGGACAAAGGGCACGTTAATGATTAC
	CAGCAGAAACTGGATAAAAAAGAACAGGAACGTGATGCTGCGCGTAAATCGTGGACCACG
	GTTGAGAACATTAAAGAGCTGAAAGAGGGGTATCTAAGCCATGTGGTACACAAACTGGCG
	CACCTCATCATTAAATATAACGCAATAGTCTGCCTAGAAGACTTGAATTTTGGCTTTAAA
	CGCGGCCGCTTCAAAGTGGAAAAACAAGTTTATCAAAAATTTGAAAAGGCGCTTATAGAT
	AAACTGAATTATCTGGTTTTTAAAGAAAAGGAACTTGGTGAGGTAGGGCACTACTTGACA
	GCTTATCAACTGACGGCCCCGTTCGAATCATTCAAAAAACTGGGCAAACAGTCTGGCATT
	CTGTTTTACGTGCCGGCAGATTATACTTCAAAAATCGATCCAACAACTGGCTTTGTGAAC
	TTCCTGGACCTGAGATATCAGTCTGTAGAAAAAGCTAAACAACTTCTTAGCGATTTTAAT
	GCCATTCGTTTTAACAGCGTTCAGAATTACTTTGAATTCGAAATTGACTATAAAAAACTT
	ACTCCGAAACGTAAAGTCGGAACCCAAAGTAAATGGGTAATTTGTACGTATGGCGATGTC
	AGGTATCAGAACCGTCGGAATCAAAAAGGTCATTGGGAGACCGAAGAAGTGAACGTGACC
	GAAAAGCTGAAGGCTCTGTTCGCCAGCGATTCAAAAACTACAACTGTGATCGATTACGCA
	AATGATGATAACCTGATAGATGTGATTTTAGAGCAGGATAAAGCCAGCTTTTTTAAAGAA
	CTGTTGTGGCTCCTGAAACTTACGATGACCTTACGACATTCCAAGATCAAATCGGAAGAT
	GATTTTATTCTGTCACCGGTCAAGAATGAGCAGGGTGAATTCTATGATAGTAGGAAAGCC
	GGCGAAGTGTGGCCGAAAGACGCCGACGCCAATGGCGCCTATCATATCGCGCTCAAAGGG
	CTTTGGAATTTGCAGCAGATTAACCAGTGGGAAAAAGGTAAAACCCTGAATCTGGCTATC
	AAAAACCAGGATTGGTTTAGCTTTATCCAAGAGAAACCGTATCAGGAATGAGAAATCATC
	CTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTTATTATATCGCGTTGATTATTGAT
	GCTGTTTTTAGTTTTAACGGCAATTAATATATGTGTTATTAATTGAATGAATTTTATCAT
	TCATAATAAGTATGTGTAGGATCAAGCTCAGGTTAAATATTCACTCAGGAAGTTATTACT
	CAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAGTGTTAAGGGATGTTATTTCC

SEQ	AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID	TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO:	CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
65	TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
	ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
	TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
	GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
	ACCATCATACAGGCGGTCTTCTTAGTATGGACGCGAAAGAGTTCACAGGTCAGTATCCGT
	TGTCGAAAACATTACGATTCGAACTTCGGCCCATCGGCCGCACGTGGGATAACCTGGAGG
	CCTCAGGCTACTTAGCGGAAGACCGCCATCGTGCCGAATGTTATCCTCGTGCGAAAGAGT
	TATTGGATGACAACCATCGTGCCTTCCTGAATCGTGTGTTGCCACAAATCGATATGGATT
	GGCACCCGATTGCGGAGGCCTTTTGTAAGGTACATAAAAACCCTGGTAATAAAGAACTTG
	CCCAGGATTACAACCTTCAGTTGTCAAAGCGCCGTAAGGAGATCAGCGCATATCTTCAGG
	ATGCAGATGGCTATAAAGGCCTGTTCGCGAAGCCCGCCTTAGACGAAGCTATGAAAATTG
	CGAAAGAAAACGGGAACGAAAGTGATATTGAGGTTCTCGAAGCGTTTAACGGTTTTAGCG
	TATACTTCACCGGTTATCATGAGTCACGCGAGAACATTTATAGCGATGAGGATATGGTGA
	GCGTAGCCTACCGAATTACTGAGGATAATTTCCCGCGCTTTGTCTCAAACGCTTTGATCT
	TTGATAAATTAAACGAAAGCCATCCGGATATTATCTCTGAAGTATCGGGCAATCTTGGAG
	TTGATGACATTGGTAAGTACTTTGACGTGTCGAACTATAACAATTTTCTTTCCCAGGCCG
	GTATAGATGACTACAATCACATTATTGGCGGCCATACAACCGAAGACGGACTGATACAAG
	CGTTTAATGTCGTATTGAACTTACGTCACCAAAAAGACCCTGGCTTTGAAAAAATTCAGT
	TCAAACAGCTCTACAAACAAATCCTGAGCGTGCGTACCAGCAAAAGCTACATCCCGAAAC
	AGTTTGACAACTCTAAGGAGATGGTTGACTGCATTTGCGATTATGTCAGCAAAATAGAGA
	AATCCGAAACAGTAGAACGGGCCCTGAAACTAGTCCGTAATATCAGTTCTTTCGACTTGC
	GCGGGATCTTTGTCAATAAAAAGAACTTGCGCATACTGAGCAACAAACTGATAGGAGATT
	GGGACGCGATCGAAACCGCATTGATGCATAGTTCTTCATCAGAAAACGATAAGAAAAGCG
	TATATGATAGCGCGGAGGCTTTTACGTTGGATGACATCTTTTCAAGCGTGAAAAAATTTT
	CTGATGCCTCTGCCGAAGATATTGGCAACAGGGCGGAAGACATCTGTAGAGTGATAAGTG
	AGACGGCCCCTTTTATCAACGATCTGCGAGCGGTGGACCTGGATAGCCTGAACGACGATG
	GTTATGAAGCGGCCGTCTCAAAAATTCGGGAGTCGCTGGAGCCTTATATGGATCTTTTCC
	ATGAACTGGAAATTTTCTCGGTTGGCGATGAGTTCCCAAAATGCGCAGCATTTTACAGCG
	AACTGGAGGAAGTCAGCGAACAGCTGATCGAAATTATTCCGTTATTCAACAAGGCGCGTT
	CGTTCTGCACCCGGAAACGCTATAGCACCGATAAGATTAAAGTGAACTTAAAATTCCCGA
	CCTTGGCGGACGGGTGGGACCTGAACAAAGAGAGAGACAACAAAGCCGCGATTCTGCGGA
	AAGACGGTAAGTATTATCTGGCAATTCTGGATATGAAGAAAGATCTGTCAAGCATTAGGA
	CCAGCGACGAAGATGAATCCAGCTTCGAAAAGATGGAGTATAAACTGTTACCGAGTCCAG
	TAAAAATGCTGCCAAAGATATTCGTAAAATCGAAAGCCGCTAAGGAAAAATATGGCCTGA
	CAGATCGTATGCTTGAATGCTACGATAAAGGTATGCATAAGTCGGGTAGTGCGTTTGATC
	TTGGCTTTTGCCATGAACTCATTGATTATTACAAGCGTTGTATCGCGGAGTACCCAGGCT
	GGGATGTGTTCGATTTCAAGTTTCGCGAAACTTCCGATTATGGGTCCATGAAAGAGTTCA
	ATGAAGATGTGGCCGGAGCCGGTTACTATATGAGTCTGAGAAAAATTCCGTGCAGCGAAG
	TGTACCGTCTGTTAGACGAGAAATCGATTTATCTATTTCAAATTTATAACAAAGATTACT
	CTGAAAATGCACATGGTAATAAGAACATGCATACCATGTACTGGGAGGGTCTCTTTTCCC
	CGCAAAACCTGGAGTCGCCCGTTTTCAAGTTGTCGGGTGGGGCAGAACTTTTCTTTCGAA
	AATCCTCAATCCCTAACGATGCCAAAACAGTACACCCGAAAGGCTCAGTGCTGGTTCCAC
	GTAATGATGTTAACGGTCGGCGTATTCCAGATTCAATCTACCGCGAACTGACACGCTATT
	TTAACCGTGGCGATTGCCGAATCAGTGACGAAGCCAAAAGTTATCTTGACAAGGTTAAGA
	CTAAAAAAGCGGACCATGACATTGTGAAAGATCGCCGCTTTACCGTGGATAAAATGATGT
	TCCACGTCCCGATTGCGATGAACTTTAAGGCGATCAGTAAACCGAACTTAAACAAAAAAG
	TCATTGATGGCATCATTGATGATCAGGATCTGAAAATCATTGGTATTGATCGTGGCGAGC
	GGAACTTAATTTACGTCACGATGGTTGACAGAAAAGGGAATATCTTATATCAGGATTCTC
	TTAACATCCTCAATGGCTACGACTATCGTAAAGCTCTGGATGTGCGCGAATATGACAACA
	AGGAAGCGCGTCGTAACTGGACTAAAGTGGAGGGCATTCGCAAAATGAAGGAAGGCTATC
	TGTCATTAGCGGTCTCGAAATTAGCGGATATGATTATCGAAAATAACGCCATCATCGTTA
	TGGAGGACCTGAACCACGGATTCAAAGCGGGCCGCTCAAAGATTGAAAAACAAGTTTATC
	AGAAATTTGAGAGTATGCTGATTAACAAACTGGGCTATATGGTGTTAAAAGACAAGTCAA
	TTGACCAATCAGGTGGCGCGCTGCATGGATACCAGCTGGCGAACCATGTTACCACCTTAG
	CATCAGTTGGAAAGCAGTGTGGGGTTATCTTTTATATACCGGCAGCGTTCACTAGTAAAA
	TAGATCCGACCACTGGTTTCGCCGATCTCTTTGCCCTGAGTAACGTTAAAAACGTAGCGA
	GCATGCGTGAATTCTTTTCCAAAATGAAATCTGTCATTTATGATAAAGCTGAAGGCAAAT
	TCGCATTCACCTTTGATTACTTGGATTACAACGTGAAGAGCGAATGTGGTCGTACGCTGT
	GGACCGTTTACACCGTTGGTGAGCGCTTCACCTATTCCCGTGTGAACCGCGAATATGTAC
	GTAAAGTCCCCACCGATATTATCTATGATGCCCTCCAGAAAGCAGGCATTAGCGTCGAAG
	GAGACTTAAGGGACAGAATTGCCGAAAGCGATGGCGATACGCTGAAGTCTATTTTTTACG
	CATTCAAATACGCGCTAGATATGCGCGTTGAGAATCGCGAGGAAGACTACATTCAATCAC
	CTGTGAAAAATGCCTCTGGGGAATTTTTTTGTTCAAAAAATGCTGGTAAAAGCCTCCCAC
	AAGATAGCGATGCAAACGGTGCATATAACATTGCCCTGAAAGGTATTCTTCAATTACGCA
	TGCTGTCTGAGCAGTACGACCCCAACGCGGAATCTATTAGACTTCCGCTGATAACCAATA
	AAGCCTGGCTGACATTCATGCAGTCTGGCATGAAGACCTGGAAAAATTAGGAAATCATCC
	TTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATGTAGGGAGACCCTCAGGTTAAATATT
	CACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACA

SEQ	AAAATTCcatGCAAAATGCTCCGGTTTCATGTCATCAAAATGATGACGTAATTAAGCATT
ID	GATAATTGAGATCCCTCTCCCTGACAGGATGATTACATAAATAATAGTGACAAAAATAAA
NO:	TTATTTATTTATCCAGAAAATGAATTGGAAAATCAGGAGAGCGTTTTCAATCCTACCTCT
66	GGCGCAGTTGATATGTcaaaCAGGTtgccgtcactgcgtcttttactggctcttctcgct
	aaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccat
	gacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
	gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctac
	ctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcac
	cgcctatctcgtgtgagataggcggagatacgaactttaagAAGGAGatataccatgGAT
	AGTTTGAAAGATTTCACCAATCTGTACCCTGTCAGTAAGACATTGAGATTTGAATTAAAG
	CCCGTTGGAAAGACTTTAGAAAATATCGAGAAAGCAGGTATTTTGAAAGAGGATGAGCAT
	CGTGCAGAAAGTTATCGGAGGGTGAAGAAAATAATTGATACTTATCATAAGGTATTTATC
	GATTCTTCTCTTGAAAATATGGCTAAAATGGGTATTGAGAATGAAATAAAAGCAATGCTC
	CAAAGTTTCTGCGAATTGTATAAAAAAGATCATCGCACTGAGGGTGAAGACAAGGCATTA
	GATAAAATTCGAGCAGTACTTCGTGGCCTGATTGTTGGGGCTTTCACTGGTGTTTGCGGA
	AGACGGGAAAATACAGTCCAAAACGAGAAGTACGAGAGTTTGTTCAAAGAAAAGTTGATA
	AAAGAAATTTTACCTGATTTTGTGCTCTCTACTGAGGCTGAAAGCTTGCCTTTCTCTGTT
	GAAGAAGCTACGAGGTCACTGAAGGAGTTTGATAGCTTTACATCCTACTTTGCTGGTTTT
	TACGAGAATAGAAAGAATATATACTCGACGAAACCTCAATCCACTGCCATTGCTTATCGT
	CTTATTCATGAGAACTTGCCGAAGTTCATTGATAATATTCTTGTTTTTCAGAAGATCAAA
	GAGCCTATAGCCAAAGAGCTGGAACATATTCGTGCGGACTTTTCTGCCGGGGGGTACATA
	AAAAAGGATGAGAGATTGGAGGATATTTTTTCGTTGAACTATTATATCCACGTGTTATCT
	CAGGCTGGGATCGAAAAATATAACGCATTGATTGGGAAGATTGTGACAGAAGGAGATGGA
	GAGATGAAAGGGCTCAATGAACACATCAACCTTTACAACCAACAAAGAGGCAGAGAGGAT
	CGGCTCCCTCTTTTTAGGCCTCTTTATAAACAGATATTGAGTGACAGAGAGCAATTATCA
	TACTTGCCTGAGAGTtttGAAAAAGAtGAGGAGctcctcAGGGctctAAAAGAGttctAt
	GAtcAtAtcGcAGAAGACATTCTCGGACGTACTCAACAGTTGATGACTTCTATTTCAGAA
	TATGATTTATCTCGGATATACGTAAGGAACGATAGCCAATTGACTGATATATCAAAAAAA
	ATGTTGGGAGATTGGAATGCTATCTACATGGCTAGAGAACGAGCATATGACCACGAGCAG
	GCTCCCAAAAGAATCACGGCGAAATACGAGAGGGACAGGATTAAAGCTCTTAAAGGAGAA
	GAGAGTATAAGTCTGGCAAATCTTAATAGTTGTATTGCCTTTCTGGACAATGTTAGAGAT
	TGCCGTGTAGATACTTATCTTTCCACACTGGGCCAGAAGGAAGGACCACATGGTCTATCT
	AATCTCGTTGAGAACGTTTTTGCCTCATACCATGAAGCAGAGCAATTGTTGAGCTTTCCA
	TACCCCGAAGAGAATAATCTGATTCAGGACAAGGACAATGTGGTGTTAATTAAGAATCTT
	CTCGACAATATCAGTGATCTGCAGAGGTTCTTGAAACCTCTTTGGGGTATGGGAGACGAA
	CCCGATAAAGATGAAAGATTTTATGGAGAGTATAATTATATCCGAGGAGCTCTAGATCAG
	GTGATCCCTCTGTACAATAAGGTAAGGAACTACCTCACTCGGAAGCCTTATTCGACCAGA
	AAAGTAAAACTCAATTTTGGGAATTCTCAATTGCTTAGTGGTTGGGATAGAAATAAGGAA
	AAGGATAATAGCTGTGTGATTTTGCGTAAGGGGCAGAACTTCTATTTGGCTATTATGAAC
	AATAGGCACAAAAGAAGTTTCGAAAACAAGGTGTTGCCCGAGTATAAGGAGGGAGAACCT
	TACTTCGAAAAGATGGATTATAAATTTTTGCCTGATCCTAATAAAATGCTTCCTAAGGTT
	TTTCTTTCGAAAAAAGGAATAGAGATATACAAACCAAGTCCGAAGCTTTTAGAACAATAT
	GGACATGGAACTCACAAAAAGGGAGATACCTTTAGTATGGATGATTTGCACGAACTGATC
	GATTTCTTCAAACACTCAATCGAGGCTCATGAAGATTGGAAGCAATTCGGATTCAAATTT
	TCTGATACGGCTACTTATGAGAATGTATCTAGTTTCTATAGAGAAGTTGAGGATCAGGGG
	TATAAGCTCTCTTTCCGAAAAGTTTCGGAATCTTATGTCTATTCATTAATAGATCAAGGC
	AAGTTGTATTTATTTCAGATATACAACAAGGACTTTTCTCCCTGCAGCAAAGGGACACCT
	AATCTGCATACCTTGTATTGGAGAATGCTTTTTGACGAGCGCAATTTGGCAGATGTCATA
	TACAAACTGGATGGGAAGGCTGAAATCTTTTTCCGAGAGAAGAGTTTGAAAAATGATCAT
	CCCACGCATCCGGCTGGTAAGCCTATCAAAAAGAAAAGTCGACAAAAAAAAGGAGAGGAG
	AGTCTGTTTGAGTATGATTTAGTCAAGGATAGGCACTATACGATGGATAAGTTCCAGTTT
	CATGTGCCTATTACTATGAATTTTAAATGTTCTGCAGGAAGCAAAGTCAATGATATGGTT
	AATGCTCATATTCGAGAGGCAAAGGATATGCATGTCATTGGAATTGATCGTGGAGAACGC
	AATCTGCTGTATATATGCGTGATAGATAGTCGAGGGACGATTTTGGATCAAATTTCTCTG
	AATACGATTAACGATATAGACTATCATGATTTATTGGAGAGTCGAGACAAAGACCGTCAG
	CAGGAGCGCCGAAACTGGCAAACTATCGAAGGGATCAAGGAGCTAAAACAAGGCTACCTT
	AGTCAGGCGGTTCATCGGATAGCCGAACTGATGGTGGCTTATAAGGCTGTAGTTGCTTTG
	GAGGATTTGAATATGGGGTTCAAACGTGGGCGGCAGAAAGTAGAAAGTTCTGTTTATCAG
	CAGTTTGAGAAACAGCTGATAGATAAGCTCAACTATCTTGTGGACAAGAAGAAAAGGCCT
	GAAGATATTGGAGGATTGTTGAGAGCCTATCAATTTACGGCCCCATTTAAGAGTTTTAAG
	GAAATGGGAAAGCAAAACGGCTTCTTGTTTTATATCCCGGCTTGGAACACGAGCAACATA
	GATCCGACTACTGGATTTGTTAATTTATTTCATGCCCAGTATGAAAATGTAGATAAAGCG
	AAGAGCTTCTTTCAAAAGTTTGATTCAATTAGTTACAACCCGAAGAAAGACTGGTTTGAG
	TTTGCATTCGATTATAAAAACTTTACTAAAAAGGCTGAAGGAAGTCGTTCTATGTGGATA
	TTATGCACACATGGTTCCCGAATAAAGAATTTTAGAAATTCCCAGAAGAATGGTCAATGG
	GATTCCGAAGAATTCGCCTTGACGGAGGCTTTTAAGTCTCTTTTTGTGCGATATGAGATA
	GATTATACCGCTGATTTGAAAACAGCTATTGTGGACGAAAAGCAAAAAGACTTCTTCGTG
	GATCTTCTGAAGCTATTCAAATTGACAGTACAGATGCGCAACAGCTGGAAAGAGAAGGAT
	TTGGATTATCTAATCTCTCCTGTAGCAGGGGCTGATGGCCGTTTCTTCGATACAAGAGAG
	GGAAATAAAAGTCTGCCTAAGGATGCAGATGCCAATGGAGCTTATAATATTGCCCTAAAA
	GGACTTTGGGCTCTACGCCAGATTCGGCAAACTTCAGAAGGCGGTAAACTCAAATTGGCG
	ATTTCCAATAAGGAATGGCTACAGTTTGTGCAAGAGAGATCTTACGAGAAAGACtgaGAA
	ATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTTATTATATCGCGTTGATT
	ATTGATGCTGTTTTTAGTTTTAACGGCAATTAATATATGTGTTATTAATTGAATGAATTT
	TATCATTCATAATAAGTATGTGTAGGATCAAGCTCAGGTTAAATATTCACTCAGGAAGTT
	ATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAGTGTTAAGGGATGTTA
	TTTCC

SEQ	AAAATTCcatGCAAAATGCTCCGGTTTCATGTCATCAAAATGATGACGTAATTAAGCATT
ID	GATAATTGAGATCCCTCTCCCTGACAGGATGATTACATAAATAATAGTGACAAAAATAAA
NO:	TTATTTATTTATCCAGAAAATGAATTGGAAAATCAGGAGAGCGTTTTCAATCCTACCTCT
67	GGCGCAGTTGATATGTcaaaCAGGTtgccgtcactgcgtcttttactggctcttctcgct
	aaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccat
	gacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
	gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctac
	ctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcac
	cgcctatctcgtgtgagataggcggagatacgaactttaagAAGGAGatataccATGAAC
	AACGGCACAAATAATTTTCAGAACTTCATCGGGATCTCAAGTTTGCAGAAAACGCTGCGC
	AATGCTCTGATCCCCACGGAAACCACGCAACAGTTCATCGTCAAGAACGGAATAATTAAA
	GAAGATGAGTTACGTGGCGAGAACCGCCAGATTCTGAAAGATATCATGGATGACTACTAC
	CGCGGATTCATCTCTGAGACTCTGAGTTCTATTGATGACATAGATTGGACTAGCCTGTTC
	GAAAAAATGGAAATTCAGCTGAAAAATGGTGATAATAAAGATACCTTAATTAAGGAACAG
	ACAGAGTATCGGAAAGCAATCCATAAAAAATTTGCGAACGACGATCGGTTTAAGAACATG
	TTTAGCGCCAAACTGATTAGTGACATATTACCTGAATTTGTCATCCACAACAATAATTAT
	TCGGCATCAGAGAAAGAGGAAAAAACCCAGGTGATAAAATTGTTTTCGCGCTTTGCGACT
	AGCTTTAAAGATTACTTCAAGAACCGTGCAAATTGCTTTTCAGCGGACGATATTTCATCA
	AGCAGCTGCCATCGCATCGTCAACGACAATGCAGAGATATTCTTTTCAAATGCGCTGGTC
	TACCGCCGGATCGTAAAATCGCTGAGCAATGACGATATCAACAAAATTTCGGGCGATATG
	AAAGATTCATTAAAAGAAATGAGTCTGGAAGAAATATATTCTTACGAGAAGTATGGGGAA
	TTTATTACCCAGGAAGGCATTAGCTTCTATAATGATATCTGTGGGAAAGTGAATTCTTTT
	ATGAACCTGTATTGTCAGAAAAATAAAGAAAACAAAAATTTATACAAACTTCAGAAACTT
	CACAAACAGATTCTATGCATTGCGGACACTAGCTATGAGGTCCCGTATAAATTTGAAAGT
	GACGAGGAAGTGTACCAATCAGTTAACGGCTTCCTTGATAACATTAGCAGCAAACATATA
	GTCGAAAGATTACGCAAAATCGGCGATAACTATAACGGCTACAACCTGGATAAAATTTAT
	ATCGTGTCCAAATTTTACGAGAGCGTTAGCCAAAAAACCTACCGCGACTGGGAAACAATT
	AATACCGCCCTCGAAATTCATTACAATAATATCTTGCCGGGTAACGGTAAAAGTAAAGCC
	GACAAAGTAAAAAAAGCGGTTAAGAATGATTTACAGAAATCCATCACCGAAATAAATGAA
	CTAGTGTCAAACTATAAGCTGTGCAGTGACGACAACATCAAAGCGGAGACTTATATACAT
	GAGATTAGCCATATCTTGAATAACTTTGAAGCACAGGAATTGAAATACAATCCGGAAATT
	CACCTAGTTGAATCCGAGCTCAAAGCGAGTGAGCTTAAAAACGTGCTGGACGTGATCATG
	AATGCGTTTCATTGGTGTTCGGTTTTTATGACTGAGGAACTTGTTGATAAAGACAACAAT
	TTTTATGCGGAACTGGAGGAGATTTACGATGAAATTTATCCAGTAATTAGTCTGTACAAC
	CTGGTTCGTAACTACGTTACCCAGAAACCGTACAGCACGAAAAAGATTAAATTGAACTTT
	GGAATACCGACGTTAGCAGACGGTTGGTCAAAGTCCAAAGAGTATTCTAATAACGCTATC
	ATACTGATGCGCGACAATCTGTATTATCTGGGCATCTTTAATGCGAAGAATAAACCGGAC
	AAGAAGATTATCGAGGGTAATACGTCAGAAAATAAGGGTGACTACAAAAAGATGATTTAT
	AATTTGCTCCCGGGTCCCAACAAAATGATCCCGAAAGTTTTCTTGAGCAGCAAGACGGGG
	GTGGAAACGTATAAACCGAGCGCCTATATCCTAGAGGGGTATAAACAGAATAAACATATC
	AAGTCTTCAAAAGACTTTGATATCACTTTCTGTCATGATCTGATCGACTACTTCAAAAAC
	TGTATTGCAATTCATCCCGAGTGGAAAAACTTCGGTTTTGATTTTAGCGACACCAGTACT
	TATGAAGACATTTCCGGGTTTTATCGTGAGGTAGAGTTACAAGGTTACAAGATTGATTGG
	ACATACATTAGCGAAAAAGACATTGATCTGCTGCAGGAAAAAGGTCAACTGTATCTGTTC
	CAGATATATAACAAAGATTTTTCGAAAAAATCAACCGGGAATGACAACCTTCACACCATG
	TACCTGAAAAATCTTTTCTCAGAAGAAAATCTTAAGGATATCGTCCTGAAACTTAACGGC
	GAAGCGGAAATCTTCTTCAGGAAGAGCAGCATAAAGAACCCAATCATTCATAAAAAAGGC
	TCGATTTTAGTCAACCGTACCTACGAAGCAGAAGAAAAAGACCAGTTTGGCAACATTCAA
	ATTGTGCGTAAAAATATTCCGGAAAACATTTATCAGGAGCTGTACAAATACTTCAACGAT
	AAAAGCGACAAAGAGCTGTCTGATGAAGCAGCCAAACTGAAGAATGTAGTGGGACACCAC
	GAGGCAGCGACGAATATAGTCAAGGACTATCGCTACACGTATGATAAATACTTCCTTCAT
	ATGCCTATTACGATCAATTTCAAAGCCAATAAAACGGGTTTTATTAATGATAGGATCTTA
	CAGTATATCGCTAAAGAAAAAGACTTACATGTGATCGGCATTGATCGGGGCGAGCGTAAC
	CTGATCTACGTGTCCGTGATTGATACTTGTGGTAATATAGTTGAACAGAAAAGCTTTAAC
	ATTGTAAACGGCTACGACTATCAGATAAAACTGAAACAACAGGAGGGCGCTAGACAGATT
	GCGCGGAAAGAATGGAAAGAAATTGGTAAAATTAAAGAGATCAAAGAGGGCTACCTGAGC
	TTAGTAATCCACGAGATCTCTAAAATGGTAATCAAATACAATGCAATTATAGCGATGGAG
	GATTTGTCTTATGGTTTTAAAAAAGGGCGCTTTAAGGTCGAACGGCAAGTTTACCAGAAA
	TTTGAAACCATGCTCATCAATAAACTCAACTATCTGGTATTTAAAGATATTTCGATTACC
	GAGAATGGCGGTCTCCTGAAAGGTTATCAGCTGACATACATTCCTGATAAACTTAAAAAC
	GTGGGTCATCAGTGCGGCTGCATTTTTTATGTGCCTGCTGCATACACGAGCAAAATTGAT
	CCGACCACCGGCTTTGTGAATATCTTTAAATTTAAAGACCTGACAGTGGACGCAAAACGT
	GAATTCATTAAAAAATTTGACTCAATTCGTTATGACAGTGAAAAAAATCTGTTCTGCTTT
	ACATTTGACTACAATAACTTTATTACGCAAAACACGGTCATGAGCAAATCATCGTGGAGT
	GTGTATACATACGGCGTGCGCATCAAACGTCGCTTTGTGAACGGCCGCTTCTCAAACGAA
	AGTGATACCATTGACATAACCAAAGATATGGAGAAAACGTTGGAAATGACGGACATTAAC
	TGGCGCGATGGCCACGATCTTCGTCAAGACATTATAGATTATGAAATTGTTCAGCACATA
	TTCGAAATTTTCCGTTTAACAGTGCAAATGCGTAACTCCTTGTCTGAACTGGAGGACCGT
	GATTACGATCGTCTCATTTCACCTGTACTGAACGAAAATAACATTTTTTATGACAGCGCG
	AAAGCGGGGGATGCACTTCCTAAGGATGCCGATGCAAATGGTGCGTATTGTATTGCATTA
	AAAGGGTTATATGAAATTAAACAAATTACCGAAAATTGGAAAGAAGATGGTAAATTTTCG
	CGCGATAAACTCAAAATCAGCAATAAAGATTGGTTCGACTTTATCCAGAATAAGCGCTAT
	CTCTAAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTTATTATATC
	GCGTTGATTATTGATGCTGTTTTTAGTTTTAACGGCAATTAATATATGTGTTATTAATTG
	AATGAATTTTATCATTCATAATAAGTATGTGTAGGATCAAGCTCAGGTTAAATATTCACT
	CAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAGTGTTAA
	GGGATGTTATTTCC

SEQ	AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID	TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO:	CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
68	TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
	ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
	TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
	GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
	ACCATACCAATAAATTCACTAACCAGTATTCTCTCTCTAAGACCCTGCGCTTTGAACTGA
	TTCCGCAGGGGAAAACCTTGGAGTTCATTCAAGAAAAAGGCCTCTTGTCTCAGGATAAAC
	AGAGGGCTGAATCTTACCAAGAAATGAAGAAAACTATTGATAAGTTTCATAAATATTTCA
	TTGATTTAGCCTTGTCTAACGCCAAATTAACTCACTTGGAAACGTATCTGGAGTTATACA
	ACAAATCTGCCGAAACTAAGAAAGAACAGAAATTTAAAGACGATTTGAAAAAAGTACAGG
	ACAATCTGCGTAAAGAAATTGTCAAATCCTTCAGTGACGGCGATGCTAAAAGCATTTTTG
	CCATTCTGGACAAAAAAGAGTTGATTACTGTGGAATTAGAAAAGTGGTTTGAAAACAATG
	AGCAGAAAGACATCTACTTCGATGAGAAATTCAAAACTTTCACCACCTATTTTACAGGAT
	TTCATCAAAACCGGAAGAACATGTACTCAGTAGAACCGAACTCCACGGCCATTGCGTATC
	GTTTGATCCATGAGAATCTGCCTAAATTTCTGGAGAATGCGAAAGCCTTTGAAAAGATTA
	AGCAGGTCGAATCGCTGCAAGTGAATTTTCGTGAACTCATGGGCGAATTTGGTGACGAAG
	GTCTAATCTTCGTTAACGAACTGGAAGAAATGTTTCAGATTAATTACTACAATGACGTGC
	TATCGCAGAACGGTATCACAATCTACAATAGTATTATCTCAGGGTTCACAAAAAACGATA
	TAAAATACAAAGGCCTGAACGAGTATATCAATAACTACAACCAAACAAAGGACAAAAAGG
	ATAGGCTTCCGAAACTGAAGCAGTTATACAAACAGATTTTATCTGACAGAATCTCCCTGA
	GCTTTCTGCCGGATGCTTTCACTGATGGGAAGCAGGTTCTGAAAGCGATTTTCGATTTTT
	ATAAGATTAACTTACTGAGCTACACGATTGAAGGTCAAGAAGAATCTCAAAACTTACTGC
	TCTTGATCCGTCAAACCATTGAAAATCTATCATCGTTCGATACGCAGAAAATCTACCTCA
	AAAACGATACTCACCTGACTACGATCTCTCAGCAGGTTTTCGGGGATTTTAGTGTATTTT
	CAACAGCTCTGAACTACTGGTATGAAACCAAAGTCAATCCGAAATTCGAGACGGAATATT
	CTAAGGCCAACGAAAAAAAACGTGAGATTCTTGATAAAGCTAAAGCCGTATTTACTAAAC
	AGGATTACTTTTCTATTGCTTTCCTGCAGGAAGTTTTATCGGAGTATATCCTGACCCTGG
	ATCATACATCTGATATCGTTAAAAAACACAGCAGCAATTGCATCGCTGACTATTTCAAAA
	ACCACTTTGTCGCCAAAAAAGAAAACGAAACAGACAAGACTTTCGATTTCATTGCTAACA
	TCACCGCAAAATACCAGTGTATTCAGGGTATCTTGGAAAACGCCGACCAATACGAAGACG
	AACTGAAACAAGATCAGAAGCTGATCGATAATTTAAAATTCTTCTTAGATGCAATCCTGG
	AGCTGCTGCACTTCATCAAACCGCTTCATTTAAAGAGCGAGTCCATTACCGAAAAGGACA
	CCGCCTTCTATGACGTTTTTGAAAATTATTATGAAGCCCTCTCCTTGCTGACTCCGCTGT
	ATAATATGGTACGCAATTACGTAACCCAGAAACCATATTCTACCGAAAAAATTAAACTGA
	ACTTTGAAAACGCACAGCTGCTCAACGGTTGGGACGCGAATAAAGAAGGTGACTACCTCA
	CCACCATCCTGAAAAAAGATGGTAACTATTTTCTGGCAATTATGGATAAGAAACATAATA
	AAGCATTCCAGAAATTTCCTGAAGGGAAAGAAAATTACGAAAAGATGGTGTACAAACTCT
	TACCTGGAGTTAACAAAATGTTGCCGAAAGTATTTTTTAGTAATAAGAACATCGCGTACT
	TTAACCCGTCCAAAGAACTGCTGGAAAATTATAAAAAGGAGACGCATAAGAAAGGGGATA
	CCTTTAACCTGGAACATTGCCATACCTTAATAGACTTCTTCAAGGATTCCCTGAATAAAC
	ACGAGGATTGGAAATATTTCGATTTTCAGTTTAGTGAGACCAAGTCATACCAGGATCTTA
	GCGGCTTTTATCGCGAAGTAGAACACCAAGGCTATAAAATTAACTTCAAAAACATCGACA
	GCGAATACATCGACGGTTTAGTTAACGAGGGCAAACTGTTTCTGTTCCAGATCTATTCAA
	AGGATTTTAGCCCGTTCTCTAAAGGCAAACCAAATATGCATACGTTGTACTGGAAAGCAC
	TGTTTGAAGAGCAAAACCTGCAGAATGTGATTTATAAACTGAACGGCCAAGCTGAGATTT
	TTTTCCGTAAAGCCTCGATTAAACCGAAAAATATCATCCTTCATAAGAAGAAAATAAAGA
	TCGCTAAAAAACACTTCATAGATAAAAAAACCAAAACCTCCGAAATAGTGCCTGTTCAAA
	CAATTAAGAACTTGAATATGTACTACCAGGGCAAGATATCGGAAAAGGAGTTGACTCAAG
	ACGATCTTCGCTATATCGATAACTTTTCGATTTTTAACGAAAAAAACAAGACGATCGACA
	TCATCAAAGATAAACGCTTCACTGTAGATAAGTTCCAGTTTCATGTGCCGATTACTATGA
	ACTTCAAAGCTACCGGGGGTAGCTATATCAACCAAACGGTGTTGGAATACCTGCAGAATA
	ACCCGGAAGTCAAAATCATTGGGCTGGACCGCGGAGAACGTCACCTTGTGTACTTGACCT
	TAATCGATCAGCAAGGCAACATCTTAAAACAAGAATCGCTGAATACCATTACGGATTCAA
	AGATTAGCACCCCGTATCATAAGCTGCTCGATAACAAGGAGAATGAGCGCGACCTGGCCC
	GTAAAAACTGGGGCACGGTGGAAAACATTAAGGAGTTAAAGGAGGGTTATATTTCCCAGG
	TAGTGCATAAGATCGCCACTCTCATGCTCGAGGAAAATGCGATCGTTGTCATGGAAGACT
	TAAACTTCGGATTTAAACGTGGGCGATTTAAAGTAGAGAAACAAATCTACCAGAAGTTAG
	AAAAAATGCTGATTGACAAATTAAATTACTTGGTCCTAAAAGACAAACAGCCGCAAGAAT
	TGGGTGGATTATACAACGCCCTCCAACTTACCAATAAATTCGAAAGTTTTCAGAAAATGG
	GTAAACAGTCAGGCTTTCTTTTTTATGTTCCTGCGTGGAACACATCCAAAATCGACCCTA
	CAACCGGCTTCGTCAATTACTTCTATACTAAATATGAAAACGTCGACAAAGCAAAAGCAT
	TCTTTGAAAAGTTCGAAGCAATACGTTTTAACGCTGAGAAAAAATATTTCGAGTTCGAAG
	TCAAGAAATACTCAGACTTTAACCCCAAAGCTGAGGGCACACAGCAAGCGTGGACAATCT
	GCACCTACGGCGAGCGCATCGAAACGAAGCGTCAAAAAGATCAGAATAACAAATTTGTTT
	CAACACCTATCAACCTGACCGAGAAGATTGAAGACTTCTTAGGTAAAAATCAGATTGTTT
	ATGGCGACGGTAACTGTATAAAATCTCAAATAGCCTCAAAGGATGATAAAGCATTTTTCG
	AAACATTATTATATTGGTTCAAAATGACACTGCAGATGCGCAATAGTGAGACGCGTACAG
	ATATTGATTATCTTATCAGCCCGGTCATGAACGACAACGGTACTTTTTACAACTCCAGAG
	ACTATGAAAAACTTGAGAATCCAACTCTCCCCAAAGATGCTGATGCGAACGGTGCTTATC
	ACATCGCGAAAAAAGGTCTGATGCTGCTGAACAAAATCGACCAAGCCGATCTGACTAAGA
	AAGTTGACCTAAGCATTTCAAATCGGGACTGGTTACAGTTTGTTCAAAAGAACAAATGAG
	AAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATGTAGGGAGACCCTCAGG
	TTAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACA

SEQ	AAAATTCcatGCAAAATGCTCCGGTTTCATGTCATCAAAATGATGACGTAATTAAGCATT
ID	GATAATTGAGATCCCTCTCCCTGACAGGATGATTACATAAATAATAGTGACAAAAATAAA
NO:	TTATTTATTTATCCAGAAAATGAATTGGAAAATCAGGAGAGCGTTTTCAATCCTACCTCT
69	GGCGCAGTTGATATGTcaaaCAGGTtgccgtcactgcgtcttttactggctcttctcgct
	aaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccat
	gacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
	gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctac
	ctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcac
	cgcctatctcgtgtgagataggcggagatacgaactttaagAAGGAGatataccATGGAA
	CAGGAATATTATCTGGGCTTGGACATGGGCACCGGTTCCGTCGGCTGGGCTGTTACTGAC
	AGTGAATATCACGTTCTAAGAAAGCATGGTAAGGCATTGTGGGGTGTAAGACTTTTCGAA
	TCTGCTTCCACTGCTGAAGAGCGTAGAATGTTTAGAACGAGTCGACGTAGGCTAGACAGG
	CGCAATTGGAGAATCGAAATTTTACAAGAAATTTTTGCGGAAGAGATATCTAAGAAAGAC
	CCAGGCTTTTTCCTGAGAATGAAGGAATCTAAGTATTACCCTGAGGATAAAAGAGATATA
	AATGGTAACTGTCCCGAATTGCCTTACGCATTATTTGTGGACGATGATTTTACCGATAAG
	GATTACCATAAAAAGTTCCCAACTATCTACCATTTACGCAAAATGTTAATGAATACAGAG
	GAAACCCCAGACATAAGACTAGTTTATCTGGCAATACACCATATGATGAAACATAGAGGC
	CATTTCTTACTTTCCGGGGATATCAACGAAATCAAAGAGTTTGGTACCACATTTAGTAAG
	TTACTGGAAAACATAAAGAATGAAGAATTGGATTGGAACTTAGAACTCGGAAAAGAAGAA
	TACGCGGTTGTCGAATCTATCCTGAAGGATAATATGCTGAATAGGTCGACCAAAAAAACT
	AGGCTGATCAAAGCACTGAAAGCCAAATCTATCTGCGAAAAAGCTGTTTTAAATTTACTT
	GCTGGTGGCACTGTTAAGTTATCAGACATTTTTGGTTTGGAAGAATTGAACGAAACCGAG
	CGTCCAAAAATTAGTTTCGCTGATAATGGCTACGATGATTACATTGGTGAGGTGGAAAAC
	GAGTTGGGCGAACAATTTTATATTATAGAGACAGCTAAGGCAGTCTATGACTGGGCTGTT
	TTAGTAGAAATCCTTGGTAAATACACATCTATCTCCGAAGCGAAAGTTGCTACTTACGAA
	AAGCACAAGTCCGATCTCCAGTTTTTGAAGAAAATTGTCAGGAAATATCTGACTAAGGAA
	GAATATAAAGATATTTTCGTTAGTACCTCTGACAAACTGAAAAATTACTCCGCTTACATC
	GGGATGACCAAGATTAATGGCAAAAAAGTTGATCTGCAAAGCAAAAGGTGTTCGAAGGAA
	GAATTTTATGATTTCATTAAAAAGAATGTCTTAAAAAAATTAGAAGGTCAGCCAGAATAC
	GAATATTTGAAAGAAGAACTGGAAAGAGAGACATTCTTACCAAAACAAGTCAACAGAGAT
	AATGGGGTAATTCCATATCAAATTCACCTCTACGAATTAAAAAAAATTTTAGGCAATTTA
	CGCGATAAAATTGACCTTATCAAAGAAAATGAGGATAAGCTGGTTCAACTCTTTGAATTC
	AGAATACCCTATTATGTGGGCCCACTGAACAAGATTGATGACGGCAAAGAAGGTAAATTC
	ACATGGGCCGTCCGCAAATCCAATGAAAAAATTTACCCATGGAACTTTGAAAATGTAGTA
	GATATTGAAGCGTCTGCGGAGAAATTTATTCGAAGAATGACTAATAAATGCACTTACTTG
	ATGGGAGAGGATGTTCTGCCTAAAGACAGCTTATTATACAGCAAGTACATGGTTCTAAAC
	GAACTTAACAACGTTAAGTTGGACGGTGAGAAATTAAGTGTAGAATTGAAACAAAGATTG
	TATACTGACGTCTTCTGCAAGTACAGAAAAGTGACAGTTAAAAAAATTAAGAATTACTTG
	AAGTGCGAAGGTATAATTTCTGGAAACGTAGAGATTACTGGTATTGATGGTGATTTCAAA
	GCATCCCTAACAGCTTACCACGATTTCAAGGAAATCCTGACAGGAACTGAACTCGCAAAA
	AAAGATAAAGAAAACATTATTACTAATATTGTTCTTTTCGGTGATGACAAGAAATTGTTG
	AAGAAAAGACTGAATAGACTTTACCCCCAGATTACTCCCAATCAACTTAAGAAAATTTGT
	GCTTTGTCTTACACAGGATGGGGTCGTTTTTCAAAAAAGTTCTTAGAAGAGATTACCGCA
	CCTGATCCAGAAACAGGCGAAGTATGGAATATAATTACCGCCTTATGGGAATCGAACAAT
	AATCTTATGCAACTTCTGAGCAATGAATATCGTTTCATGGAAGAAGTTGAGACTTACAAC
	ATGGGCAAACAGACGAAGACTTTATCCTATGAAACTGTGGAAAATATGTATGTATCACCT
	TCTGTCAAGAGACAAATTTGGCAAACCTTAAAAATTGTCAAAGAATTAGAAAAGGTAATG
	AAGGAGTCTCCTAAACGTGTGTTTATTGAAATGGCTAGAGAAAAACAAGAGTCAAAAAGA
	ACCGAGTCAAGAAAGAAGCAGTTAATCGATTTATATAAGGCTTGTAAAAACGAAGAGAAA
	GATTGGGTTAAAGAATTGGGGGACCAAGAGGAACAAAAACTACGGTCGGATAAGTTGTAT
	TTATACTATACGCAAAAGGGACGATGTATGTATTCCGGCGAGGTAATAGAATTGAAGGAT
	TTATGGGACAATACAAAATATGACATAGACCATATATATCCCCAATCAAAAACGATGGAC
	GATAGCTTGAACAATAGAGTACTCGTGAAAAAAAAATATAATGCGACCAAATCTGATAAG
	TATCCTCTGAATGAAAATATCAGACATGAAAGAAAGGGGTTCTGGAAGTCCTTGTTAGAT
	GGTGGGTTTATAAGCAAAGAAAAGTACGAGCGTCTAATAAGAAACACGGAGTTATCGCCA
	GAAGAACTCGCTGGTTTTATTGAGAGGCAAATCGTGGAAACGAGACAATCTACCAAAGCC
	GTTGCTGAGATCCTAAAGCAAGTTTTCCCAGAGTCGGAGATTGTCTATGTCAAAGCTGGC
	ACAGTGAGCAGGTTTAGGAAAGACTTCGAACTATTAAAGGTAAGAGAAGTGAACGATTTA
	CATCACGCAAAGGACGCTTACCTAAATATCGTTGTAGGTAACTCATATTATGTTAAATTT
	ACCAAGAACGCCTCTTGGTTTATAAAGGAGAACCCAGGTAGAACATATAACCTGAAAAAG
	ATGTTCACCTCTGGTTGGAATATTGAGAGAAACGGAGAAGTCGCATGGGAAGTTGGTAAG
	AAAGGGACTATAGTGACAGTAAAGCAAATTATGAACAAAAATAATATCCTCGTTACAAGG
	CAGGTTCATGAAGCAAAGGGCGGCCTTTTTGACCAACAAATTATGAAGAAAGGGAAAGGT
	CAAATTGCAATAAAAGAAACCGATGAGAGACTAGCGTCAATAGAAAAGTATGGTGGCTAT
	AATAAAGCTGCGGGTGCATACTTTATGCTTGTTGAATCAAAAGACAAGAAAGGTAAGACT
	ATTAGAACTATAGAATTTATACCCCTGTACCTTAAAAACAAAATTGAATCGGATGAGTCA
	ATCGCGTTAAATTTTCTAGAGAAAGGAAGGGGTTTAAAAGAACCAAAGATCCTGTTAAAA
	AAGATTAAGATTGACACCTTGTTCGATGTAGATGGATTTAAAATGTGGTTATCTGGCAGA
	ACAGGCGATAGACTTTTGTTTAAGTGCGCTAATCAATTAATTTTGGATGAGAAAATCATT
	GTCACAATGAAAAAAATAGTTAAGTTTATTCAGAGAAGACAAGAAAACAGGGAGTTGAAA
	TTATCTGATAAAGATGGTATCGACAATGAAGTTTTAATGGAAATCTACAATACATTCGTT
	GATAAACTTGAAAATACCGTATATCGAATCAGGTTAAGTGAACAAGCCAAAACATTAATT
	GATAAACAAAAAGAATTTGAAAGGCTATCACTGGAAGACAAATCCTCCACCCTATTTGAA
	ATTTTGCATATATTCCAGTGCCAATCTTCAGCAGCTAATTTAAAAATGATTGGCGGACCT
	GGGAAAGCCGGCATCCTAGTGATGAACAATAATATCTCCAAGTGTAACAAAATATCAATT
	ATTAACCAATCTCCGACAGGTATTTTTGAAAATGAAATAGACTTGCTTAAGATATAAGAA
	ATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTTATTATATCGCGTTGATT
	ATTGATGCTGTTTTTAGTTTTAACGGCAATTAATATATGTGTTATTAATTGAATGAATTT
	TATCATTCATAATAAGTATGTGTAGGATCAAGCTCAGGTTAAATATTCACTCAGGAAGTT
	ATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAGTGTTAAGGGATGTTA
	TTTCC

SEQ	AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID	TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO:	CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
70	TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
	ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
	TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
	GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
	ACCATTCTTTCGACTCTTTCACCAACCTGTACTCTCTGTCTAAAACCCTGAAATTCGAAA
	TGCGTCCGGTTGGTAACACCCAGAAAATGCTGGACAACGCGGGTGTTTTCGAAAAAGACA
	AACTGATCCAGAAAAAATACGGTAAAACCAAACCGTACTTCGACCGTCTGCACCGTGAAT
	TCATCGAAGAAGCGCTGACCGGTGTTGAACTGATCGGTCTGGACGAAAACTTCCGTACCC
	TGGTTGACTGGCAGAAAGACAAAAAAAACAACGTTGCGATGAAAGCGTACGAAAACTCTC
	TGCAGCGTCTGCGTACCGAAATCGGTAAAATCTTCAACCTGAAAGCGGAAGACTGGGTTA
	AAAACAAATACCCGATCCTGGGTCTGAAAAACAAAAACACCGACATCCTGTTCGAAGAAG
	CGGTTTTCGGTATCCTGAAAGCGCGTTACGGTGAAGAAAAAGACACCTTCATCGAAGTTG
	AAGAAATCGACAAAACCGGTAAATCTAAAATCAACCAGATCTCTATCTTCGACTCTTGGA
	AAGGTTTCACCGGTTACTTCAAAAAATTCTTCGAAACCCGTAAAAACTTCTACAAAAACG
	ACGGTACCTCTACCGCGATCGCGACCCGTATCATCGACCAGAACCTGAAACGTTTCATCG
	ACAACCTGTCTATCGTTGAATCTGTTCGTCAGAAAGTTGACCTGGCGGAAACCGAAAAAT
	CTTTCTCTATCTCTCTGTCTCAGTTCTTCTCTATCGACTTCTACAACAAATGCCTGCTGC
	AGGACGGTATCGACTACTACAACAAAATCATCGGTGGTGAAACCCTGAAAAACGGTGAAA
	AACTGATCGGTCTGAACGAACTGATCAACCAGTACCGTCAGAACAACAAAGACCAGAAAA
	TCCCGTTCTTCAAACTGCTGGACAAACAGATCCTGTCTGAAAAAATCCTGTTCCTGGACG
	AAATCAAAAACGACACCGAACTGATCGAAGCGCTGTCTCAGTTCGCGAAAACCGCGGAAG
	AAAAAACCAAAATCGTTAAAAAACTGTTCGCGGACTTCGTTGAAAACAACTCTAAATACG
	ACCTGGCGCAGATCTACATCTCTCAGGAAGCGTTCAACACCATCTCTAACAAATGGACCT
	CTGAAACCGAAACCTTCGCGAAATACCTGTTCGAAGCGATGAAATCTGGTAAACTGGCGA
	AATACGAAAAAAAAGACAACTCTTACAAATTCCCGGACTTCATCGCGCTGTCTCAGATGA
	AATCTGCGCTGCTGTCTATCTCTCTGGAAGGTCACTTCTGGAAAGAAAAATACTACAAAA
	TCTCTAAATTCCAGGAAAAAACCAACTGGGAACAGTTCCTGGCGATCTTCCTGTACGAAT
	TCAACTCTCTGTTCTCTGACAAAATCAACACCAAAGACGGTGAAACCAAACAGGTTGGTT
	ACTACCTGTTCGCGAAAGACCTGCACAACCTGATCCTGTCTGAACAGATCGACATCCCGA
	AAGACTCTAAAGTTACCATCAAAGACTTCGCGGACTCTGTTCTGACCATCTACCAGATGG
	CGAAATACTTCGCGGTTGAAAAAAAACGTGCGTGGCTGGCGGAATACGAACTGGACTCTT
	TCTACACCCAGCCGGACACCGGTTACCTGCAGTTCTACGACAACGCGTACGAAGACATCG
	TTCAGGTTTACAACAAACTGCGTAACTACCTGACCAAAAAACCGTACTCTGAAGAAAAAT
	GGAAACTGAACTTCGAAAACTCTACCCTGGCGAACGGTTGGGACAAAAACAAAGAATCTG
	ACAACTCTGCGGTTATCCTGCAGAAAGGTGGTAAATACTACCTGGGTCTGATCACCAAAG
	GTCACAACAAAATCTTCGACGACCGTTTCCAGGAAAAATTCATCGTTGGTATCGAAGGTG
	GTAAATACGAAAAAATCGTTTACAAATTCTTCCCGGACCAGGCGAAAATGTTCCCGAAAG
	TTTGCTTCTCTGCGAAAGGTCTGGAATTCTTCCGTCCGTCTGAAGAAATCCTGCGTATCT
	ACAACAACGCGGAATTCAAAAAAGGTGAAACCTACTCTATCGACTCTATGCAGAAACTGA
	TCGACTTCTACAAAGACTGCCTGACCAAATACGAAGGTTGGGCGTGCTACACCTTCCGTC
	ACCTGAAACCGACCGAAGAATACCAGAACAACATCGGTGAATTCTTCCGTGACGTTGCGG
	AAGACGGTTACCGTATCGACTTCCAGGGTATCTCTGACCAGTACATCCACGAAAAAAACG
	AAAAAGGTGAACTGCACCTGTTCGAAATCCACAACAAAGACTGGAACCTGGACAAAGCGC
	GTGACGGTAAATCTAAAACCACCCAGAAAAACCTGCACACCCTGTACTTCGAATCTCTGT
	TCTCTAACGACAACGTTGTTCAGAACTTCCCGATCAAACTGAACGGTCAGGCGGAAATCT
	TCTACCGTCCGAAAACCGAAAAAGACAAACTGGAATCTAAAAAAGACAAAAAAGGTAACA
	AAGTTATCGACCACAAACGTTACTCTGAAAACAAAATCTTCTTCCACGTTCCGCTGACCC
	TGAACCGTACCAAAAACGACTCTTACCGTTTCAACGCGCAGATCAACAACTTCCTGGCGA
	ACAACAAAGACATCAACATCATCGGTGTTGACCGTGGTGAAAAACACCTGGTTTACTACT
	CTGTTATCACCCAGGCGTCTGACATCCTGGAATCTGGTTCTCTGAACGAACTGAACGGTG
	TTAACTACGCGGAAAAACTGGGTAAAAAAGCGGAAAACCGTGAACAGGCGCGTCGTGACT
	GGCAGGACGTTCAGGGTATCAAAGACCTGAAAAAAGGTTACATCTCTCAGGTTGTTCGTA
	AACTGGCGGACCTGGCGATCAAACACAACGCGATCATCATCCTGGAAGACCTGAACATGC
	GTTTCAAACAGGTTCGTGGTGGTATCGAAAAATCTATCTACCAGCAGCTGGAAAAAGCGC
	TGATCGACAAACTGTCTTTCCTGGTTGACAAAGGTGAAAAAAACCCGGAACAGGCGGGTC
	ACCTGCTGAAAGCGTACCAGCTGTCTGCGCCGTTCGAAACCTTCCAGAAAATGGGTAAAC
	AGACCGGTATCATCTTCTACACCCAGGCGTCTTACACCTCTAAATCTGACCCGGTTACCG
	GTTGGCGTCCGCACCTGTACCTGAAATACTTCTCTGCGAAAAAAGCGAAAGACGACATCG
	CGAAATTCACCAAAATCGAATTCGTTAACGACCGTTTCGAACTGACCTACGACATCAAAG
	ACTTCCAGCAGGCGAAAGAATACCCGAACAAAACCGTTTGGAAAGTTTGCTCTAACGTTG
	AACGTTTCCGTTGGGACAAAAACCTGAACCAGAACAAAGGTGGTTACACCCACTACACCA
	ACATCACCGAAAACATCCAGGAACTGTTCACCAAATACGGTATCGACATCACCAAAGACC
	TGCTGACCCAGATCTCTACCATCGACGAAAAACAGAACACCTCTTTCTTCCGTGACTTCA
	TCTTCTACTTCAACCTGATCTGCCAGATCCGTAACACCGACGACTCTGAAATCGCGAAAA
	AAAACGGTAAAGACGACTTCATCCTGTCTCCGGTTGAACCGTTCTTCGACTCTCGTAAAG
	ACAACGGTAACAAACTGCCGGAAAACGGTGACGACAACGGTGCGTACAACATCGCGCGTA
	AAGGTATCGTTATCCTGAACAAAATCTCTCAGTACTCTGAAAAAAACGAAAACTGCGAAA
	AAATGAAATGGGGTGACCTGTACGTTTCTAACATCGACTGGGACAACTTCGTTGAAATCA
	TCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATGTAGGGAGACCCTCAGGTTAAAT
	ATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACA

SEQ	AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID	TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO:	CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
71	TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
	ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
	TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
	GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
	ACCATAACAAATTCGAAAACTTCACCGGTCTGTACCCGATCTCTAAAACCCTGCGTTTCG
	AACTGATCCCGCAGGGTAAAACCCTGGAATACATCGAAAAATCTGAAATCCTGGAAAACG
	ACAACTACCGTGCGGAAAAATACGAAGAAGTTAAAGACATCATCGACGGTTACCACAAAT
	GGTTCATCAACGAAACCCTGCACGACCTGCACATCAACTGGTCTGAACTGAAAGTTGCGC
	TGGAAAACAACCGTATCGAAAAATCTGACGCGTCTAAAAAAGAACTGCAGCGTGTTCAGA
	AAATCAAACGTGAAGAAATCTACAACGCGTTCATCGAACACGAAGCGTTCCAGTACCTGT
	TCAAAGAAAACCTGCTGTCTGACCTGCTGCCGATCCAGATCGAACAGTCTGAAGACCTGG
	ACGCGGAAAAAAAAAAACAGGCGGTTGAAACCTTCAACCGTTTCTCTACCTACTTCACCG
	GTTTCCACGAAAACCGTAAAAACATCTACTCTAAAGAAGGTATCTCTACCTCTGTTACCT
	ACCGTATCGTTCACGACAACTTCCCGAAATTCCTGGAAAACATGAAAGTTTTCGAAATCC
	TGCGTAACGAATGCCCGGAAGTTATCTCTGACACCGCGAACGAACTGGCGCCGTTCATCG
	ACGGTGTTCGTATCGAAGACATCTTCCTGATCGACTTCTTCAACTCTACCTTCTCTCAGA
	ACGGTATCGACTACTACAACCGTATCCTGGGTGGTGTTACCACCGAAACCGGTGAAAAAT
	ACCGTGGTATCAACGAATTCACCAACCTGTACCGTCAGCAGCACCCGGAATTCGGTAAAT
	CTAAAAAAGCGACCAAAATGGTTGTTCTGTTCAAACAGATCCTGTCTGACCGTGACACCC
	TGTCTTTCATCCCGGAAATGTTCGGTAACGACAAACAGGTTCAGAACTCTATCCAGCTGT
	TCTACAACCGTGAAATCTCTCAGTTCGAAAACGAAGGTGTTAAAACCGACGTTTGCACCG
	CGCTGGCGACCCTGACCTCTAAAATCGCGGAATTCGACACCGAAAAAATCTACATCCAGC
	AGCCGGAACTGCCGAACGTTTCTCAGCGTCTGTTCGGTTCTTGGAACGAACTGAACGCGT
	GCCTGTTCAAATACGCGGAACTGAAATTCGGTACCGCGGAAAAAGTTGCGAACCGTAAAA
	AAATCGACAAATGGCTGAAATCTGACCTGTTCTCTTTCACCGAACTGAACAAAGCGCTGG
	AATTCTCTGGTAAAGACGAACGTATCGAAAACTACTTCTCTGAAACCGGTATCTTCGCGC
	AGCTGGTTAAAACCGGTTTCGACGAAGCGCAGTCTATCCTGGAAACCGAATACACCTCTG
	AAGTTCACCTGAAAGACCAGCAGACCGACATCGAAAAAATCAAAACCTTCCTGGACGCGC
	TGCAGAACCTGATGCACCTGCTGAAATCTCTGTGCGTTTCTGAAGAAGCGGACCGTGACG
	CGGCGTTCTACAACGAATTCGACATGCTGTACAACCAGCTGAAACTGGTTGTTCCGCTGT
	ACAACAAAGTTCGTAACTACATCACCCAGAAACTGTTCCGTTCTGACAAAATCAAAATCT
	ACTTCGAAAACAAAGGTCAGTTCCTGGGTGGTTGGGTTGACTCTCAGACCGAAAACTCTG
	ACAACGGTACCCAGGCGGGTGGTTACATCTTCCGTAAAGAAAACGTTATCAACGAATACG
	ACTACTACCTGGGTATCTGCTCTGACCCGAAACTGTTCCGTCGTACCACCATCGTTTCTG
	AAAACGACCGTTCTTCTTTCGAACGTCTGGACTACTACCAGCTGAAAACCGCGTCTGTTT
	ACGGTAACTCTTACTGCGGTAAACACCCGTACACCGAAGACAAAAACGAACTGGTTAACT
	CTATCGACCGTTTCGTTCACCTGTCTGGTAACAACATCCTGATCGAAAAAATCGCGAAAG
	ACAAAGTTAAATCTAACCCGACCACCAACACCCCGTCTGGTTACCTGAACTTCATCCACC
	GTGAAGCGCCGAACACCTACGAATGCCTGCTGCAGGACGAAAACTTCGTTTCTCTGAACC
	AGCGTGTTGTTTCTGCGCTGAAAGCGACCCTGGCGACCCTGGTTCGTGTTCCGAAAGCGC
	TGGTTTACGCGAAAAAAGACTACCACCTGTTCTCTGAAATCATCAACGACATCGACGAAC
	TGTCTTACGAAAAAGCGTTCTCTTACTTCCCGGTTTCTCAGACCGAATTCGAAAACTCTT
	CTAACCGTACCATCAAACCGCTGCTGCTGTTCAAAATCTCTAACAAAGACCTGTCTTTCG
	CGGAAAACTTCGAAAAAGGTAACCGTCAGAAAATCGGTAAAAAAAACCTGCACACCCTGT
	ACTTCGAAGCGCTGATGAAAGGTAACCAGGACACCATCGACATCGGTACCGGTATGGTTT
	TCCACCGTGTTAAATCTCTGAACTACAACGAAAAAACCCTGAAATACGGTCACCACTCTA
	CCCAGCTGAACGAAAAATTCTCTTACCCGATCATCAAAGACAAACGTTTCGCGTCTGACA
	AATTCCTGTTCCACCTGTCTACCGAAATCAACTACAAAGAAAAACGTAAACCGCTGAACA
	ACTCTATCATCGAATTCCTGACCAACAACCCGGACATCAACATCATCGGTCTGGACCGTG
	GTGAACGTCACCTGATCTACCTGACCCTGATCAACCAGAAAGGTGAAATCCTGCGTCAGA
	AAACCTTCAACATCGTTGGTAACACCAACTACCACGAAAAACTGAACCAGCGTGAAAAAG
	AACGTGACAACGCGCGTAAATCTTGGGCGACCATCGGTAAAATCAAAGAACTGAAAGAAG
	GTTTCCTGTCTCTGGTTATCCACGAAATCGCGAAAATCATGGTTGAAAACAACGCGATCG
	TTGTTCTGGAAGACCTGAACTTCGGTTTCAAACGTGGTCGTTTCAAAGTTGAAAAACAGA
	TCTACCAGAAATTCGAAAAAATGCTGATCGACAAACTGAACTACCTGGTTTTCAAAGACA
	AAAAAGCGAACGAAGCGGGTGGTGTTCTGAAAGGTTACCAGCTGGCGGAAAAATTCGAAT
	CTTTCCAGAAAATGGGTAAACAGTCTGGTTTCCTGTTCTACGTTCCGGCGGCGTACACCT
	CTAAAATCGACCCGACCACCGGTTTCGTTAACATGCTGAACCTGAACTACACCAACATGA
	AAGACGCGCAGACCCTGCTGTCTGGTATGGACAAAATCTCTTTCAACGCGGACGCGAACT
	ACTTCGAATTCGAACTGGACTACGAAAAATTCAAAACCAACCAGACCGACCACACCAACA
	AATGGACCATCTGCACCGTTGGTGAAAAACGTTTCACCTACAACTCTGCGACCAAAGAAA
	CCACCACCGTTAACGTTACCGAAGACCTGAAAAAACTGCTGGACAAATTCGAAGTTAAAT
	ACTCTAACGGTGACAACATCAAAGACGAAATCTGCCGTCAGACCGACGCGAAATTCTTCG
	AAATCATCCTGTGGCTGCTGAAACTGACCATGCAGATGCGTAACTCTAACACCAAAACCG
	AAGAAGACTTCATCCTGTCTCCGGTTAAAAACTCTAACGGTGAATTCTTCCGTTCTAACG
	ACGACGCGAACGGTATCTGGCCGGCGGACGCGGACGCGAACGGTGCGTACCACATCGCGC
	TGAAAGGTCTGTACCTGGTTAAAGAATGCTTCAACAAAAACGAAAAATCTCTGAAAATCG
	AACACAAAAACTGGTTCAAATTCGCGCAGACCCGTTTCAACGGTTCTCTGACCAAAAACG
	GTTAAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATGTAGGGAGACC
	CTCAGGTTAAATATTCACTCAGGAAGTTATTACTCAGGAAGCAAAGAGGATTACA

SEQ	AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID	TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO:	CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
72	TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
	ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
	TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
	GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
	ACCATACCCAGTTCGAAGGTTTCACCAACCTGTACCAGGTTTCTAAAACCCTGCGTTTCG
	AACTGATCCCGCAGGGTAAAACCCTGAAACACATCCAGGAACAGGGTTTCATCGAAGAAG
	ACAAAGCGCGTAACGACCACTACAAAGAACTGAAACCGATCATCGACCGTATCTACAAAA
	CCTACGCGGACCAGTGCCTGCAGCTGGTTCAGCTGGACTGGGAAAACCTGTCTGCGGCGA
	TCGACTCTTACCGTAAAGAAAAAACCGAAGAAACCCGTAACGCGCTGATCGAAGAACAGG
	CGACCTACCGTAACGCGATCCACGACTACTTCATCGGTCGTACCGACAACCTGACCGACG
	CGATCAACAAACGTCACGCGGAAATCTACAAAGGTCTGTTCAAAGCGGAACTGTTCAACG
	GTAAAGTTCTGAAACAGCTGGGTACCGTTACCACCACCGAACACGAAAACGCGCTGCTGC
	GTTCTTTCGACAAATTCACCACCTACTTCTCTGGTTTCTACGAAAACCGTAAAAACGTTT
	TCTCTGCGGAAGACATCTCTACCGCGATCCCGCACCGTATCGTTCAGGACAACTTCCCGA
	AATTCAAAGAAAACTGCCACATCTTCACCCGTCTGATCACCGCGGTTCCGTCTCTGCGTG
	AACACTTCGAAAACGTTAAAAAAGCGATCGGTATCTTCGTTTCTACCTCTATCGAAGAAG
	TTTTCTCTTTCCCGTTCTACAACCAGCTGCTGACCCAGACCCAGATCGACCTGTACAACC
	AGCTGCTGGGTGGTATCTCTCGTGAAGCGGGTACCGAAAAAATCAAAGGTCTGAACGAAG
	TTCTGAACCTGGCGATCCAGAAAAACGACGAAACCGCGCACATCATCGCGTCTCTGCCGC
	ACCGTTTCATCCCGCTGTTCAAACAGATCCTGTCTGACCGTAACACCCTGTCTTTCATCC
	TGGAAGAATTCAAATCTGACGAAGAAGTTATCCAGTCTTTCTGCAAATACAAAACCCTGC
	TGCGTAACGAAAACGTTCTGGAAACCGCGGAAGCGCTGTTCAACGAACTGAACTCTATCG
	ACCTGACCCACATCTTCATCTCTCACAAAAAACTGGAAACCATCTCTTCTGCGCTGTGCG
	ACCACTGGGACACCCTGCGTAACGCGCTGTACGAACGTCGTATCTCTGAACTGACCGGTA
	AAATCACCAAATCTGCGAAAGAAAAAGTTCAGCGTTCTCTGAAACACGAAGACATCAACC
	TGCAGGAAATCATCTCTGCGGCGGGTAAAGAACTGTCTGAAGCGTTCAAACAGAAAACCT
	CTGAAATCCTGTCTCACGCGCACGCGGCGCTGGACCAGCCGCTGCCGACCACCCTGAAAA
	AACAGGAAGAAAAAGAAATCCTGAAATCTCAGCTGGACTCTCTGCTGGGTCTGTACCACC
	TGCTGGACTGGTTCGCGGTTGACGAATCTAACGAAGTTGACCCGGAATTCTCTGCGCGTC
	TGACCGGTATCAAACTGGAAATGGAACCGTCTCTGTCTTTCTACAACAAAGCGCGTAACT
	ACGCGACCAAAAAACCGTACTCTGTTGAAAAATTCAAACTGAACTTCCAGATGCCGACCC
	TGGCGTCTGGTTGGGACGTTAACAAAGAAAAAAACAACGGTGCGATCCTGTTCGTTAAAA
	ACGGTCTGTACTACCTGGGTATCATGCCGAAACAGAAAGGTCGTTACAAAGCGCTGTCTT
	TCGAACCGACCGAAAAAACCTCTGAAGGTTTCGACAAAATGTACTACGACTACTTCCCGG
	ACGCGGCGAAAATGATCCCGAAATGCTCTACCCAGCTGAAAGCGGTTACCGCGCACTTCC
	AGACCCACACCACCCCGATCCTGCTGTCTAACAACTTCATCGAACCGCTGGAAATCACCA
	AAGAAATCTACGACCTGAACAACCCGGAAAAAGAACCGAAAAAATTCCAGACCGCGTACG
	CGAAAAAAACCGGTGACCAGAAAGGTTACCGTGAAGCGCTGTGCAAATGGATCGACTTCA
	CCCGTGACTTCCTGTCTAAATACACCAAAACCACCTCTATCGACCTGTCTTCTCTGCGTC
	CGTCTTCTCAGTACAAAGACCTGGGTGAATACTACGCGGAACTGAACCCGCTGCTGTACC
	ACATCTCTTTCCAGCGTATCGCGGAAAAAGAAATCATGGACGCGGTTGAAACCGGTAAAC
	TGTACCTGTTCCAGATCTACAACAAAGACTTCGCGAAAGGTCACCACGGTAAACCGAACC
	TGCACACCCTGTACTGGACCGGTCTGTTCTCTCCGGAAAACCTGGCGAAAACCTCTATCA
	AACTGAACGGTCAGGCGGAACTGTTCTACCGTCCGAAATCTCGTATGAAACGTATGGCGC
	ACCGTCTGGGTGAAAAAATGCTGAACAAAAAACTGAAAGACCAGAAAACCCCGATCCCGG
	ACACCCTGTACCAGGAACTGTACGACTACGTTAACCACCGTCTGTCTCACGACCTGTCTG
	ACGAAGCGCGTGCGCTGCTGCCGAACGTTATCACCAAAGAAGTTTCTCACGAAATCATCA
	AAGACCGTCGTTTCACCTCTGACAAATTCTTCTTCCACGTTCCGATCACCCTGAACTACC
	AGGCGGCGAACTCTCCGTCTAAATTCAACCAGCGTGTTAACGCGTACCTGAAAGAACACC
	CGGAAACCCCGATCATCGGTATCGACCGTGGTGAACGTAACCTGATCTACATCACCGTTA
	TCGACTCTACCGGTAAAATCCTGGAACAGCGTTCTCTGAACACCATCCAGCAGTTCGACT
	ACCAGAAAAAACTGGACAACCGTGAAAAAGAACGTGTTGCGGCGCGTCAGGCGTGGTCTG
	TTGTTGGTACCATCAAAGACCTGAAACAGGGTTACCTGTCTCAGGTTATCCACGAAATCG
	TTGACCTGATGATCCACTACCAGGCGGTTGTTGTTCTGGAAAACCTGAACTTCGGTTTCA
	AATCTAAACGTACCGGTATCGCGGAAAAAGCGGTTTACCAGCAGTTCGAAAAAATGCTGA
	TCGACAAACTGAACTGCCTGGTTCTGAAAGACTACCCGGCGGAAAAAGTTGGTGGTGTTC
	TGAACCCGTACCAGCTGACCGACCAGTTCACCTCTTTCGCGAAAATGGGTACCCAGTCTG
	GTTTCCTGTTCTACGTTCCGGCGCCGTACACCTCTAAAATCGACCCGCTGACCGGTTTCG
	TTGACCCGTTCGTTTGGAAAACCATCAAAAACCACGAATCTCGTAAACACTTCCTGGAAG
	GTTTCGACTTCCTGCACTACGACGTTAAAACCGGTGACTTCATCCTGCACTTCAAAATGA
	ACCGTAACCTGTCTTTCCAGCGTGGTCTGCCGGGTTTCATGCCGGCGTGGGACATCGTTT
	TCGAAAAAAACGAAACCCAGTTCGACGCGAAAGGTACCCCGTTCATCGCGGGTAAACGTA
	TCGTTCCGGTTATCGAAAACCACCGTTTCACCGGTCGTTACCGTGACCTGTACCCGGCGA
	ACGAACTGATCGCGCTGCTGGAAGAAAAAGGTATCGTTTTCCGTGACGGTTCTAACATCC
	TGCCGAAACTGCTGGAAAACGACGACTCTCACGCGATCGACACCATGGTTGCGCTGATCC
	GTTCTGTTCTGCAGATGCGTAACTCTAACGCGGCGACCGGTGAAGACTACATCAACTCTC
	CGGTTCGTGACCTGAACGGTGTTTGCTTCGACTCTCGTTTCCAGAACCCGGAATGGCCGA
	TGGACGCGGACGCGAACGGTGCGTACCACATCGCGCTGAAAGGTCAGCTGCTGCTGAACC
	ACCTGAAAGAATCTAAAGACCTGAAACTGCAGAACGGTATCTCTAACCAGGACTGGCTGG
	CGTACATCCAGGAACTGCGTAACTAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTT
	ATCTGAAATGTAGGGAGACCCTCAGGTTAAATATTCACTCAGGAAGTTATTACTCAGGAA
	GCAAAGAGGATTACA

SEQ	AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID	TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO:	CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
73	TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
	ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
	TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
	GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
	ACCATGCGGTTAAATCTATCAAAGTTAAACTGCGTCTGGACGACATGCCGGAAATCCGTG
	CGGGTCTGTGGAAACTGCACAAAGAAGTTAACGCGGGTGTTCGTTACTACACCGAATGGC
	TGTCTCTGCTGCGTCAGGAAAACCTGTACCGTCGTTCTCCGAACGGTGACGGTGAACAGG
	AATGCGACAAAACCGCGGAAGAATGCAAAGCGGAACTGCTGGAACGTCTGCGTGCGCGTC
	AGGTTGAAAACGGTCACCGTGGTCCGGCGGGTTCTGACGACGAACTGCTGCAGCTGGCGC
	GTCAGCTGTACGAACTGCTGGTTCCGCAGGCGATCGGTGCGAAAGGTGACGCGCAGCAGA
	TCGCGCGTAAATTCCTGTCTCCGCTGGCGGACAAAGACGCGGTTGGTGGTCTGGGTATCG
	CGAAAGCGGGTAACAAACCGCGTTGGGTTCGTATGCGTGAAGCGGGTGAACCGGGTTGGG
	AAGAAGAAAAAGAAAAAGCGGAAACCCGTAAATCTGCGGACCGTACCGCGGACGTTCTGC
	GTGCGCTGGCGGACTTCGGTCTGAAACCGCTGATGCGTGTTTACACCGACTCTGAAATGT
	CTTCTGTTGAATGGAAACCGCTGCGTAAAGGTCAGGCGGTTCGTACCTGGGACCGTGACA
	TGTTCCAGCAGGCGATCGAACGTATGATGTCTTGGGAATCTTGGAACCAGCGTGTTGGTC
	AGGAATACGCGAAACTGGTTGAACAGAAAAACCGTTTCGAACAGAAAAACTTCGTTGGTC
	AGGAACACCTGGTTCACCTGGTTAACCAGCTGCAGCAGGACATGAAAGAAGCGTCTCCGG
	GTCTGGAATCTAAAGAACAGACCGCGCACTACGTTACCGGTCGTGCGCTGCGTGGTTCTG
	ACAAAGTTTTCGAAAAATGGGGTAAACTGGCGCCGGACGCGCCGTTCGACCTGTACGACG
	CGGAAATCAAAAACGTTCAGCGTCGTAACACCCGTCGTTTCGGTTCTCACGACCTGTTCG
	CGAAACTGGCGGAACCGGAATACCAGGCGCTGTGGCGTGAAGACGCGTCTTTCCTGACCC
	GTTACGCGGTTTACAACTCTATCCTGCGTAAACTGAACCACGCGAAAATGTTCGCGACCT
	TCACCCTGCCGGACGCGACCGCGCACCCGATCTGGACCCGTTTCGACAAACTGGGTGGTA
	ACCTGCACCAGTACACCTTCCTGTTCAACGAATTCGGTGAACGTCGTCACGCGATCCGTT
	TCCACAAACTGCTGAAAGTTGAAAACGGTGTTGCGCGTGAAGTTGACGACGTTACCGTTC
	CGATCTCTATGTCTGAACAGCTGGACAACCTGCTGCCGCGTGACCCGAACGAACCGATCG
	CGCTGTACTTCCGTGACTACGGTGCGGAACAGCACTTCACCGGTGAATTCGGTGGTGCGA
	AAATCCAGTGCCGTCGTGACCAGCTGGCGCACATGCACCGTCGTCGTGGTGCGCGTGACG
	TTTACCTGAACGTTTCTGTTCGTGTTCAGTCTCAGTCTGAAGCGCGTGGTGAACGTCGTC
	CGCCGTACGCGGCGGTTTTCCGTCTGGTTGGTGACAACCACCGTGCGTTCGTTCACTTCG
	ACAAACTGTCTGACTACCTGGCGGAACACCCGGACGACGGTAAACTGGGTTCTGAAGGTC
	TGCTGTCTGGTCTGCGTGTTATGTCTGTTGACCTGGGTCTGCGTACCTCTGCGTCTATCT
	CTGTTTTCCGTGTTGCGCGTAAAGACGAACTGAAACCGAACTCTAAAGGTCGTGTTCCGT
	TCTTCTTCCCGATCAAAGGTAACGACAACCTGGTTGCGGTTCACGAACGTTCTCAGCTGC
	TGAAACTGCCGGGTGAAACCGAATCTAAAGACCTGCGTGCGATCCGTGAAGAACGTCAGC
	GTACCCTGCGTCAGCTGCGTACCCAGCTGGCGTACCTGCGTCTGCTGGTTCGTTGCGGTT
	CTGAAGACGTTGGTCGTCGTGAACGTTCTTGGGCGAAACTGATCGAACAGCCGGTTGACG
	CGGCGAACCACATGACCCCGGACTGGCGTGAAGCGTTCGAAAACGAACTGCAGAAACTGA
	AATCTCTGCACGGTATCTGCTCTGACAAAGAATGGATGGACGCGGTTTACGAATCTGTTC
	GTCGTGTTTGGCGTCACATGGGTAAACAGGTTCGTGACTGGCGTAAAGACGTTCGTTCTG
	GTGAACGTCCGAAAATCCGTGGTTACGCGAAAGACGTTGTTGGTGGTAACTCTATCGAAC
	AGATCGAATACCTGGAACGTCAGTACAAATTCCTGAAATCTTGGTCTTTCTTCGGTAAAG
	TTTCTGGTCAGGTTATCCGTGCGGAAAAAGGTTCTCGTTTCGCGATCACCCTGCGTGAAC
	ACATCGACCACGCGAAAGAAGACCGTCTGAAAAAACTGGCGGACCGTATCATCATGGAAG
	CGCTGGGTTACGTTTACGCGCTGGACGAACGTGGTAAAGGTAAATGGGTTGCGAAATACC
	CGCCGTGCCAGCTGATCCTGCTGGAAGAACTGTCTGAATACCAGTTCAACAACGACCGTC
	CGCCGTCTGAAAACAACCAGCTGATGCAGTGGTCTCACCGTGGTGTTTTCCAGGAACTGA
	TCAACCAGGCGCAGGTTCACGACCTGCTGGTTGGTACCATGTACGCGGCGTTCTCTTCTC
	GTTTCGACGCGCGTACCGGTGCGCCGGGTATCCGTTGCCGTCGTGTTCCGGCGCGTTGCA
	CCCAGGAACACAACCCGGAACCGTTCCCGTGGTGGCTGAACAAATTCGTTGTTGAACACA
	CCCTGGACGCGTGCCCGCTGCGTGCGGACGACCTGATCCCGACCGGTGAAGGTGAAATCT
	TCGTTTCTCCGTTCTCTGCGGAAGAAGGTGACTTCCACCAGATCCACGCGGACCTGAACG
	CGGCGCAGAACCTGCAGCAGCGTCTGTGGTCTGACTTCGACATCTCTCAGATCCGTCTGC
	GTTGCGACTGGGGTGAAGTTGACGGTGAACTGGTTCTGATCCCGCGTCTGACCGGTAAAC
	GTACCGCGGACTCTTACTCTAACAAAGTTTTCTACACCAACACCGGTGTTACCTACTACG
	AACGTGAACGTGGTAAAAAACGTCGTAAAGTTTTCGCGCAGGAAAAACTGTCTGAAGAAG
	AAGCGGAACTGCTGGTTGAAGCGGACGAAGCGCGTGAAAAATCTGTTGTTCTGATGCGTG
	ACCCGTCTGGTATCATCAACCGTGGTAACTGGACCCGTCAGAAAGAATTCTGGTCTATGG
	TTAACCAGCGTATCGAAGGTTACCTGGTTAAACAGATCCGTTCTCGTGTTCCGCTGCAGG
	ACTCTGCGTGCGAAAACACCGGTGACATCTAAGAAATCATCCTTAGCGAAAGCTAAGGAT
	TTTTTTTATCTGAAATGTAGGGAGACCCTCAGGTTAAATATTCACTCAGGAAGTTATTAC
	TCAGGAAGCAAAGAGGATTACA

SEQ	AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID	TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO:	CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
74	TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
	ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
	TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
	GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
	ACCATGCGACCCGTTCTTTCATCCTGAAAATCGAACCGAACGAAGAAGTTAAAAAAGGTC
	TGTGGAAAACCCACGAAGTTCTGAACCACGGTATCGCGTACTACATGAACATCCTGAAAC
	TGATCCGTCAGGAAGCGATCTACGAACACCACGAACAGGACCCGAAAAACCCGAAAAAAG
	TTTCTAAAGCGGAAATCCAGGCGGAACTGTGGGACTTCGTTCTGAAAATGCAGAAATGCA
	ACTCTTTCACCCACGAAGTTGACAAAGACGTTGTTTTCAACATCCTGCGTGAACTGTACG
	AAGAACTGGTTCCGTCTTCTGTTGAAAAAAAAGGTGAAGCGAACCAGCTGTCTAACAAAT
	TCCTGTACCCGCTGGTTGACCCGAACTCTCAGTCTGGTAAAGGTACCGCGTCTTCTGGTC
	GTAAACCGCGTTGGTACAACCTGAAAATCGCGGGTGACCCGTCTTGGGAAGAAGAAAAAA
	AAAAATGGGAAGAAGACAAAAAAAAAGACCCGCTGGCGAAAATCCTGGGTAAACTGGCGG
	AATACGGTCTGATCCCGCTGTTCATCCCGTTCACCGACTCTAACGAACCGATCGTTAAAG
	AAATCAAATGGATGGAAAAATCTCGTAACCAGTCTGTTCGTCGTCTGGACAAAGACATGT
	TCATCCAGGCGCTGGAACGTTTCCTGTCTTGGGAATCTTGGAACCTGAAAGTTAAAGAAG
	AATACGAAAAAGTTGAAAAAGAACACAAAACCCTGGAAGAACGTATCAAAGAAGACATCC
	AGGCGTTCAAATCTCTGGAACAGTACGAAAAAGAACGTCAGGAACAGCTGCTGCGTGACA
	CCCTGAACACCAACGAATACCGTCTGTCTAAACGTGGTCTGCGTGGTTGGCGTGAAATCA
	TCCAGAAATGGCTGAAAATGGACGAAAACGAACCGTCTGAAAAATACCTGGAAGTTTTCA
	AAGACTACCAGCGTAAACACCCGCGTGAAGCGGGTGACTACTCTGTTTACGAATTCCTGT
	CTAAAAAAGAAAACCACTTCATCTGGCGTAACCACCCGGAATACCCGTACCTGTACGCGA
	CCTTCTGCGAAATCGACAAAAAAAAAAAAGACGCGAAACAGCAGGCGACCTTCACCCTGG
	CGGACCCGATCAACCACCCGCTGTGGGTTCGTTTCGAAGAACGTTCTGGTTCTAACCTGA
	ACAAATACCGTATCCTGACCGAACAGCTGCACACCGAAAAACTGAAAAAAAAACTGACCG
	TTCAGCTGGACCGTCTGATCTACCCGACCGAATCTGGTGGTTGGGAAGAAAAAGGTAAAG
	TTGACATCGTTCTGCTGCCGTCTCGTCAGTTCTACAACCAGATCTTCCTGGACATCGAAG
	AAAAAGGTAAACACGCGTTCACCTACAAAGACGAATCTATCAAATTCCCGCTGAAAGGTA
	CCCTGGGTGGTGCGCGTGTTCAGTTCGACCGTGACCACCTGCGTCGTTACCCGCACAAAG
	TTGAATCTGGTAACGTTGGTCGTATCTACTTCAACATGACCGTTAACATCGAACCGACCG
	AATCTCCGGTTTCTAAATCTCTGAAAATCCACCGTGACGACTTCCCGAAATTCGTTAACT
	TCAAACCGAAAGAACTGACCGAATGGATCAAAGACTCTAAAGGTAAAAAACTGAAATCTG
	GTATCGAATCTCTGGAAATCGGTCTGCGTGTTATGTCTATCGACCTGGGTCAGCGTCAGG
	CGGCGGCGGCGTCTATCTTCGAAGTTGTTGACCAGAAACCGGACATCGAAGGTAAACTGT
	TCTTCCCGATCAAAGGTACCGAACTGTACGCGGTTCACCGTGCGTCTTTCAACATCAAAC
	TGCCGGGTGAAACCCTGGTTAAATCTCGTGAAGTTCTGCGTAAAGCGCGTGAAGACAACC
	TGAAACTGATGAACCAGAAACTGAACTTCCTGCGTAACGTTCTGCACTTCCAGCAGTTCG
	AAGACATCACCGAACGTGAAAAACGTGTTACCAAATGGATCTCTCGTCAGGAAAACTCTG
	ACGTTCCGCTGGTTTACCAGGACGAACTGATCCAGATCCGTGAACTGATGTACAAACCGT
	ACAAAGACTGGGTTGCGTTCCTGAAACAGCTGCACAAACGTCTGGAAGTTGAAATCGGTA
	AAGAAGTTAAACACTGGCGTAAATCTCTGTCTGACGGTCGTAAAGGTCTGTACGGTATCT
	CTCTGAAAAACATCGACGAAATCGACCGTACCCGTAAATTCCTGCTGCGTTGGTCTCTGC
	GTCCGACCGAACCGGGTGAAGTTCGTCGTCTGGAACCGGGTCAGCGTTTCGCGATCGACC
	AGCTGAACCACCTGAACGCGCTGAAAGAAGACCGTCTGAAAAAAATGGCGAACACCATCA
	TCATGCACGCGCTGGGTTACTGCTACGACGTTCGTAAAAAAAAATGGCAGGCGAAAAACC
	CGGCGTGCCAGATCATCCTGTTCGAAGACCTGTCTAACTACAACCCGTACGAAGAACGTT
	CTCGTTTCGAAAACTCTAAACTGATGAAATGGTCTCGTCGTGAAATCCCGCGTCAGGTTG
	CGCTGCAGGGTGAAATCTACGGTCTGCAGGTTGGTGAAGTTGGTGCGCAGTTCTCTTCTC
	GTTTCCACGCGAAAACCGGTTCTCCGGGTATCCGTTGCTCTGTTGTTACCAAAGAAAAAC
	TGCAGGACAACCGTTTCTTCAAAAACCTGCAGCGTGAAGGTCGTCTGACCCTGGACAAAA
	TCGCGGTTCTGAAAGAAGGTGACCTGTACCCGGACAAAGGTGGTGAAAAATTCATCTCTC
	TGTCTAAAGACCGTAAACTGGTTACCACCCACGCGGACATCAACGCGGCGCAGAACCTGC
	AGAAACGTTTCTGGACCCGTACCCACGGTTTCTACAAAGTTTACTGCAAAGCGTACCAGG
	TTGACGGTCAGACCGTTTACATCCCGGAATCTAAAGACCAGAAACAGAAAATCATCGAAG
	AATTCGGTGAAGGTTACTTCATCCTGAAAGACGGTGTTTACGAATGGGGTAACGCGGGTA
	AACTGAAAATCAAAAAAGGTTCTTCTAAACAGTCTTCTTCTGAACTGGTTGACTCTGACA
	TCCTGAAAGACTCTTTCGACCTGGCGTCTGAACTGAAAGGTGAAAAACTGATGCTGTACC
	GTGACCCGTCTGGTAACGTTTTCCCGTCTGACAAATGGATGGCGGCGGGTGTTTTCTTCG
	GTAAACTGGAACGTATCCTGATCTCTAAACTGACCAACCAGTACTCTATCTCTACCATCG
	AAGACGACTCTTCTAAACAGTCTATGTAAGAAATCATCCTTAGCGAAAGCTAAGGATTTT
	TTTTATCTGAAATGTAGGGAGACCCTCAGGTTAAATATTCACTCAGGAAGTTATTACTCA
	GGAAGCAAAGAGGATTACA

SEQ	AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID	TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO:	CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
75	TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
	ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
	TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
	GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
	ACCATCCGACCCGTACCATCAACCTGAAACTGGTTCTGGGTAAAAACCCGGAAAACGCGA
	CCCTGCGTCGTGCGCTGTTCTCTACCCACCGTCTGGTTAACCAGGCGACCAAACGTATCG
	AAGAATTCCTGCTGCTGTGCCGTGGTGAAGCGTACCGTACCGTTGACAACGAAGGTAAAG
	AAGCGGAAATCCCGCGTCACGCGGTTCAGGAAGAAGCGCTGGCGTTCGCGAAAGCGGCGC
	AGCGTCACAACGGTTGCATCTCTACCTACGAAGACCAGGAAATCCTGGACGTTCTGCGTC
	AGCTGTACGAACGTCTGGTTCCGTCTGTTAACGAAAACAACGAAGCGGGTGACGCGCAGG
	CGGCGAACGCGTGGGTTTCTCCGCTGATGTCTGCGGAATCTGAAGGTGGTCTGTCTGTTT
	ACGACAAAGTTCTGGACCCGCCGCCGGTTTGGATGAAACTGAAAGAAGAAAAAGCGCCGG
	GTTGGGAAGCGGCGTCTCAGATCTGGATCCAGTCTGACGAAGGTCAGTCTCTGCTGAACA
	AACCGGGTTCTCCGCCGCGTTGGATCCGTAAACTGCGTTCTGGTCAGCCGTGGCAGGACG
	ACTTCGTTTCTGACCAGAAAAAAAAACAGGACGAACTGACCAAAGGTAACGCGCCGCTGA
	TCAAACAGCTGAAAGAAATGGGTCTGCTGCCGCTGGTTAACCCGTTCTTCCGTCACCTGC
	TGGACCCGGAAGGTAAAGGTGTTTCTCCGTGGGACCGTCTGGCGGTTCGTGCGGCGGTTG
	CGCACTTCATCTCTTGGGAATCTTGGAACCACCGTACCCGTGCGGAATACAACTCTCTGA
	AACTGCGTCGTGACGAATTCGAAGCGGCGTCTGACGAATTCAAAGACGACTTCACCCTGC
	TGCGTCAGTACGAAGCGAAACGTCACTCTACCCTGAAATCTATCGCGCTGGCGGACGACT
	CTAACCCGTACCGTATCGGTGTTCGTTCTCTGCGTGCGTGGAACCGTGTTCGTGAAGAAT
	GGATCGACAAAGGTGCGACCGAAGAACAGCGTGTTACCATCCTGTCTAAACTGCAGACCC
	AGCTGCGTGGTAAATTCGGTGACCCGGACCTGTTCAACTGGCTGGCGCAGGACCGTCACG
	TTCACCTGTGGTCTCCGCGTGACTCTGTTACCCCGCTGGTTCGTATCAACGCGGTTGACA
	AAGTTCTGCGTCGTCGTAAACCGTACGCGCTGATGACCTTCGCGCACCCGCGTTTCCACC
	CGCGTTGGATCCTGTACGAAGCGCCGGGTGGTTCTAACCTGCGTCAGTACGCGCTGGACT
	GCACCGAAAACGCGCTGCACATCACCCTGCCGCTGCTGGTTGACGACGCGCACGGTACCT
	GGATCGAAAAAAAAATCCGTGTTCCGCTGGCGCCGTCTGGTCAGATCCAGGACCTGACCC
	TGGAAAAACTGGAAAAAAAAAAAAACCGTCTGTACTACCGTTCTGGTTTCCAGCAGTTCG
	CGGGTCTGGCGGGTGGTGCGGAAGTTCTGTTCCACCGTCCGTACATGGAACACGACGAAC
	GTTCTGAAGAATCTCTGCTGGAACGTCCGGGTGCGGTTTGGTTCAAACTGACCCTGGACG
	TTGCGACCCAGGCGCCGCCGAACTGGCTGGACGGTAAAGGTCGTGTTCGTACCCCGCCGG
	AAGTTCACCACTTCAAAACCGCGCTGTCTAACAAATCTAAACACACCCGTACCCTGCAGC
	CGGGTCTGCGTGTTCTGTCTGTTGACCTGGGTATGCGTACCTTCGCGTCTTGCTCTGTTT
	TCGAACTGATCGAAGGTAAACCGGAAACCGGTCGTGCGTTCCCGGTTGCGGACGAACGTT
	CTATGGACTCTCCGAACAAACTGTGGGCGAAACACGAACGTTCTTTCAAACTGACCCTGC
	CGGGTGAAACCCCGTCTCGTAAAGAAGAAGAAGAACGTTCTATCGCGCGTGCGGAAATCT
	ACGCGCTGAAACGTGACATCCAGCGTCTGAAATCTCTGCTGCGTCTGGGTGAAGAAGACA
	ACGACAACCGTCGTGACGCGCTGCTGGAACAGTTCTTCAAAGGTTGGGGTGAAGAAGACG
	TTGTTCCGGGTCAGGCGTTCCCGCGTTCTCTGTTCCAGGGTCTGGGTGCGGCGCCGTTCC
	GTTCTACCCCGGAACTGTGGCGTCAGCACTGCCAGACCTACTACGACAAAGCGGAAGCGT
	GCCTGGCGAAACACATCTCTGACTGGCGTAAACGTACCCGTCCGCGTCCGACCTCTCGTG
	AAATGTGGTACAAAACCCGTTCTTACCACGGTGGTAAATCTATCTGGATGCTGGAATACC
	TGGACGCGGTTCGTAAACTGCTGCTGTCTTGGTCTCTGCGTGGTCGTACCTACGGTGCGA
	TCAACCGTCAGGACACCGCGCGTTTCGGTTCTCTGGCGTCTCGTCTGCTGCACCACATCA
	ACTCTCTGAAAGAAGACCGTATCAAAACCGGTGCGGACTCTATCGTTCAGGCGGCGCGTG
	GTT
	ACATCCCGCTGCCGCACGGTAAAGGTTGGGAACAGCGTTACGAACCGTGCCAGCTGATCC
	TGTTCGAAGACCTGGCGCGTTACCGTTTCCGTGTTGACCGTCCGCGTCGTGAAAACTCTC
	AGCTGATGCAGTGGAACCACCGTGCGATCGTTGCGGAAACCACCATGCAGGCGGAACTGT
	ACGGTCAGATCGTTGAAAACACCGCGGCGGGTTTCTCTTCTCGTTTCCACGCGGCGACCG
	GTGCGCCGGGTGTTCGTTGCCGTTTCCTGCTGGAACGTGACTTCGACAACGACCTGCCGA
	AACCGTACCTGCTGCGTGAACTGTCTTGGATGCTGGGTAACACCAAAGTTGAATCTGAAG
	AAGAAAAACTGCGTCTGCTGTCTGAAAAAATCCGTCCGGGTTCTCTGGTTCCGTGGGACG
	GTGGTGAACAGTTCGCGACCCTGCACCCGAAACGTCAGACCCTGTGCGTTATCCACGCGG
	ACATGAACGCGGCGCAGAACCTGCAGCGTCGTTTCTTCGGTCGTTGCGGTGAAGCGTTCC
	GTCTGGTTTGCCAGCCGCACGGTGACGACGTTCTGCGTCTGGCGTCTACCCCGGGTGCGC
	GTCTGCTGGGTGCGCTGCAGCAGCTGGAAAACGGTCAGGGTGCGTTCGAACTGGTTCGTG
	ACATGGGTTCTACCTCTCAGATGAACCGTTTCGTTATGAAATCTCTGGGTAAAAAAAAAA
	TCAAACCGCTGCAGGACAACAACGGTGACGACGAACTGGAAGACGTTCTGTCTGTTCTGC
	CGGAAGAAGACGACACCGGTCGTATCACCGTTTTCCGTGACTCTTCTGGTATCTTCTTCC
	CGTGCAACGTTTGGATCCCGGCGAAACAGTTCTGGCCGGCGGTTCGTGCGATGATCTGGA
	AAGTTATGGCGTCTCACTCTCTGGGTTAAGAAATCATCCTTAGCGAAAGCTAAGGATTTT
	TTTTATCTGAAATGTAGGGAGACCCTCAGGTTAAATATTCACTCAGGAAGTTATTACTCA
	GGAAGCAAAGAGGATTACA

SEQ	AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID	TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO:	CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
76	TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
	ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
	TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
	GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
	ACCATACCAAACTGCGTCACCGTCAGAAAAAACTGACCCACGACTGGGCGGGTTCTAAAA
	AACGTGAAGTTCTGGGTTCTAACGGTAAACTGCAGAACCCGCTGCTGATGCCGGTTAAAA
	AAGGTCAGGTTACCGAATTCCGTAAAGCGTTCTCTGCGTACGCGCGTGCGACCAAAGGTG
	AAATGACCGACGGTCGTAAAAACATGTTCACCCACTCTTTCGAACCGTTCAAAACCAAAC
	CGTCTCTGCACCAGTGCGAACTGGCGGACAAAGCGTACCAGTCTCTGCACTCTTACCTGC
	CGGGTTCTCTGGCGCACTTCCTGCTGTCTGCGCACGCGCTGGGTTTCCGTATCTTCTCTA
	AATCTGGTGAAGCGACCGCGTTCCAGGCGTCTTCTAAAATCGAAGCGTACGAATCTAAAC
	TGGCGTCTGAACTGGCGTGCGTTGACCTGTCTATCCAGAACCTGACCATCTCTACCCTGT
	TCAACGCGCTGACCACCTCTGTTCGTGGTAAAGGTGAAGAAACCTCTGCGGACCCGCTGA
	TCGCGCGTTTCTACACCCTGCTGACCGGTAAACCGCTGTCTCGTGACACCCAGGGTCCGG
	AACGTGACCTGGCGGAAGTTATCTCTCGTAAAATCGCGTCTTCTTTCGGTACCTGGAAAG
	AAATGACCGCGAACCCGCTGCAGTCTCTGCAGTTCTTCGAAGAAGAACTGCACGCGCTGG
	ACGCGAACGTTTCTCTGTCTCCGGCGTTCGACGTTCTGATCAAAATGAACGACCTGCAGG
	GTGACCTGAAAAACCGTACCATCGTTTTCGACCCGGACGCGCCGGTTTTCGAATACAACG
	CGGAAGACCCGGCGGACATCATCATCAAACTGACCGCGCGTTACGCGAAAGAAGCGGTTA
	TCAAAAACCAGAACGTTGGTAACTACGTTAAAAACGCGATCACCACCACCAACGCGAACG
	GTCTGGGTTGGCTGCTGAACAAAGGTCTGTCTCTGCTGCCGGTTTCTACCGACGACGAAC
	TGCTGGAATTCATCGGTGTTGAACGTTCTCACCCGTCTTGCCACGCGCTGATCGAACTGA
	TCGCGCAGCTGGAAGCGCCGGAACTGTTCGAAAAAAACGTTTTCTCTGACACCCGTTCTG
	AAGTTCAGGGTATGATCGACTCTGCGGTTTCTAACCACATCGCGCGTCTGTCTTCTTCTC
	GTAACTCTCTGTCTATGGACTCTGAAGAACTGGAACGTCTGATCAAATCTTTCCAGATCC
	ACACCCCGCACTGCTCTCTGTTCATCGGTGCGCAGTCTCTGTCTCAGCAGCTGGAATCTC
	TGCCGGAAGCGCTGCAGTCTGGTGTTAACTCTGCGGACATCCTGCTGGGTTCTACCCAGT
	ACATGCTGACCAACTCTCTGGTTGAAGAATCTATCGCGACCTACCAGCGTACCCTGAACC
	GTATCAACTACCTGTCTGGTGTTGCGGGTCAGATCAACGGTGCGATCAAACGTAAAGCGA
	TCGACGGTGAAAAAATCCACCTGCCGGCGGCGTGGTCTGAACTGATCTCTCTGCCGTTCA
	TCGGTCAGCCGGTTATCGACGTTGAATCTGACCTGGCGCACCTGAAAAACCAGTACCAGA
	CCCTGTCTAACGAATTCGACACCCTGATCTCTGCGCTGCAGAAAAACTTCGACCTGAACT
	TCAACAAAGCGCTGCTGAACCGTACCCAGCACTTCGAAGCGATGTGCCGTTCTACCAAAA
	AAAACGCGCTGTCTAAACCGGAAATCGTTTCTTACCGTGACCTGCTGGCGCGTCTGACCT
	CTTGCCTGTACCGTGGTTCTCTGGTTCTGCGTCGTGCGGGTATCGAAGTTCTGAAAAAAC
	ACAAAATCTTCGAATCTAACTCTGAACTGCGTGAACACGTTCACGAACGTAAACACTTCG
	TTTTCGTTTCTCCGCTGGACCGTAAAGCGAAAAAACTGCTGCGTCTGACCGACTCTCGTC
	CGGACCTGCTGCACGTTATCGACGAAATCCTGCAGCACGACAACCTGGAAAACAAAGACC
	GTGAATCTCTGTGGCTGGTTCGTTCTGGTTACCTGCTGGCGGGTCTGCCGGACCAGCTGT
	CTTCTTCTTTCATCAACCTGCCGATCATCACCCAGAAAGGTGACCGTCGTCTGATCGACC
	TGATCCAGTACGACCAGATCAACCGTGACGCGTTCGTTATGCTGGTTACCTCTGCGTTCA
	AATCTAACCTGTCTGGTCTGCAGTACCGTGCGAACAAACAGTCTTTCGTTGTTACCCGTA
	CCCTGTCTCCGTACCTGGGTTCTAAACTGGTTTACGTTCCGAAAGACAAAGACTGGCTGG
	TTCCGTCTCAGATGTTCGAAGGTCGTTTCGCGGACATCCTGCAGTCTGACTACATGGTTT
	GGAAAGACGCGGGTCGTCTGTGCGTTATCGACACCGCGAAACACCTGTCTAACATCAAAA
	AATCTGTTTTCTCTTCTGAAGAAGTTCTGGCGTTCCTGCGTGAACTGCCGCACCGTACCT
	TCATCCAGACCGAAGTTCGTGGTCTGGGTGTTAACGTTGACGGTATCGCGTTCAACAACG
	GTGACATCCCGTCTCTGAAAACCTTCTCTAACTGCGTTCAGGTTAAAGTTTCTCGTACCA
	ACACCTCTCTGGTTCAGACCCTGAACCGTTGGTTCGAAGGTGGTAAAGTTTCTCCGCCGT
	CTATCCAGTTCGAACGTGCGTACTACAAAAAAGACGACCAGATCCACGAAGACGCGGCGA
	AACGTAAAATCCGTTTCCAGATGCCGGCGACCGAACTGGTTCACGCGTCTGACGACGCGG
	GTTGGACCCCGTCTTACCTGCTGGGTATCGACCCGGGTGAATACGGTATGGGTCTGTCTC
	TGGTTTCTATCAACAACGGTGAAGTTCTGGACTCTGGTTTCATCCACATCAACTCTCTGA
	TCAACTTCGCGTCTAAAAAATCTAACCACCAGACCAAAGTTGTTCCGCGTCAGCAGTACA
	AATCTCCGTACGCGAACTACCTGGAACAGTCTAAAGACTCTGCGGCGGGTGACATCGCGC
	ACATCCTGGACCGTCTGATCTACAAACTGAACGCGCTGCCGGTTTTCGAAGCGCTGTCTG
	GTAACTCTCAGTCTGCGGCGGACCAGGTTTGGACCAAAGTTCTGTCTTTCTACACCTGGG
	GTGACAACGACGCGCAGAACTCTATCCGTAAACAGCACTGGTTCGGTGCGTCTCACTGGG
	ACATCAAAGGTATGCTGCGTCAGCCGCCGACCGAAAAAAAACCGAAACCGTACATCGCGT
	TCCCGGGTTCTCAGGTTTCTTCTTACGGTAACTCTCAGCGTTGCTCTTGCTGCGGTCGTA
	ACCCGATCGAACAGCTGCGTGAAATGGCGAAAGACACCTCTATCAAAGAACTGAAAATCC
	GTAACTCTGAAATCCAGCTGTTCGACGGTACCATCAAACTGTTCAACCCGGACCCGTCTA
	CCGTTATCGAACGTCGTCGTCACAACCTGGGTCCGTCTCGTATCCCGGTTGCGGACCGTA
	CCTTCAAAAACATCTCTCCGTCTTCTCTGGAATTCAAAGAACTGATCACCATCGTTTCTC
	GTTCTATCCGTCACTCTCCGGAATTCATCGCGAAAAAACGTGGTATCGGTTCTGAATACT
	TCTGCGCGTACTCTGACTGCAACTCTTCTCTGAACTCTGAAGCGAACGCGGCGGCGAACG
	TTGCGCAGAAATTCCAGAAACAGCTGTTCTTCGAACTGTAAGAAATCATCCTTAGCGAAA
	GCTAAGGATTTTTTTTATCTGAAATGTAGGGAGACCCTCAGGTTAAATATTCACTCAGGA
	AGTTATTACTCAGGAAGCAAAGAGGATTACA

SEQ	AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID	TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO:	CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
77	TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
	ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
	TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
	GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
	ACCATAAACGTATCCTGAACTCTCTGAAAGTTGCGGCGCTGCGTCTGCTGTTCCGTGGTA
	AAGGTTCTGAACTGGTTAAAACCGTTAAATACCCGCTGGTTTCTCCGGTTCAGGGTGCGG
	TTGAAGAACTGGCGGAAGCGATCCGTCACGACAACCTGCACCTGTTCGGTCAGAAAGAAA
	TCGTTGACCTGATGGAAAAAGACGAAGGTACCCAGGTTTACTCTGTTGTTGACTTCTGGC
	TGGACACCCTGCGTCTGGGTATGTTCTTCTCTCCGTCTGCGAACGCGCTGAAAATCACCC
	TGGGTAAATTCAACTCTGACCAGGTTTCTCCGTTCCGTAAAGTTCTGGAACAGTCTCCGT
	TCTTCCTGGCGGGTCGTCTGAAAGTTGAACCGGCGGAACGTATCCTGTCTGTTGAAATCC
	GTAAAATCGGTAAACGTGAAAACCGTGTTGAAAACTACGCGGCGGACGTTGAAACCTGCT
	TCATCGGTCAGCTGTCTTCTGACGAAAAACAGTCTATCCAGAAACTGGCGAACGACATCT
	GGGACTCTAAAGACCACGAAGAACAGCGTATGCTGAAAGCGGACTTCTTCGCGATCCCGC
	TGATCAAAGACCCGAAAGCGGTTACCGAAGAAGACCCGGAAAACGAAACCGCGGGTAAAC
	AGAAACCGCTGGAACTGTGCGTTTGCCTGGTTCCGGAACTGTACACCCGTGGTTTCGGTT
	CTATCGCGGACTTCCTGGTTCAGCGTCTGACCCTGCTGCGTGACAAAATGTCTACCGACA
	CCGCGGAAGACTGCCTGGAATACGTTGGTATCGAAGAAGAAAAAGGTAACGGTATGAACT
	CTCTGCTGGGTACCTTCCTGAAAAACCTGCAGGGTGACGGTTTCGAACAGATCTTCCAGT
	TCATGCTGGGTTCTTACGTTGGTTGGCAGGGTAAAGAAGACGTTCTGCGTGAACGTCTGG
	ACCTGCTGGCGGAAAAAGTTAAACGTCTGCCGAAACCGAAATTCGCGGGTGAATGGTCTG
	GTCACCGTATGTTCCTGCACGGTCAGCTGAAATCTTGGTCTTCTAACTTCTTCCGTCTGT
	TCAACGAAACCCGTGAACTGCTGGAATCTATCAAATCTGACATCCAGCACGCGACCATGC
	TGATCTCTTACGTTGAAGAAAAAGGTGGTTACCACCCGCAGCTGCTGTCTCAGTACCGTA
	AACTGATGGAACAGCTGCCGGCGCTGCGTACCAAAGTTCTGGACCCGGAAATCGAAATGA
	CCCACATGTCTGAAGCGGTTCGTTCTTACATCATGATCCACAAATCTGTTGCGGGTTTCC
	TGCCGGACCTGCTGGAATCTCTGGACCGTGACAAAGACCGTGAATTCCTGCTGTCTATCT
	TCCCGCGTATCCCGAAAATCGACAAAAAAACCAAAGAAATCGTTGCGTGGGAACTGCCGG
	GTGAACCGGAAGAAGGTTACCTGTTCACCGCGAACAACCTGTTCCGTAACTTCCTGGAAA
	ACCCGAAACACGTTCCGCGTTTCATGGCGGAACGTATCCCGGAAGACTGGACCCGTCTGC
	GTTCTGCGCCGGTTTGGTTCGACGGTATGGTTAAACAGTGGCAGAAAGTTGTTAACCAGC
	TGGTTGAATCTCCGGGTGCGCTGTACCAGTTCAACGAATCTTTCCTGCGTCAGCGTCTGC
	AGGCGATGCTGACCGTTTACAAACGTGACCTGCAGACCGAAAAATTCCTGAAACTGCTGG
	CGGACGTTTGCCGTCCGCTGGTTGACTTCTTCGGTCTGGGTGGTAACGACATCATCTTCA
	AATCTTGCCAGGACCCGCGTAAACAGTGGCAGACCGTTATCCCGCTGTCTGTTCCGGCGG
	ACGTTTACACCGCGTGCGAAGGTCTGGCGATCCGTCTGCGTGAAACCCTGGGTTTCGAAT
	GGAAAAACCTGAAAGGTCACGAACGTGAAGACTTCCTGCGTCTGCACCAGCTGCTGGGTA
	ACCTGCTGTTCTGGATCCGTGACGCGAAACTGGTTGTTAAACTGGAAGACTGGATGAACA
	ACCCGTGCGTTCAGGAATACGTTGAAGCGCGTAAAGCGATCGACCTGCCGCTGGAAATCT
	TCGGTTTCGAAGTTCCGATCTTCCTGAACGGTTACCTGTTCTCTGAACTGCGTCAGCTGG
	AACTGCTGCTGCGTCGTAAATCTGTTATGACCTCTTACTCTGTTAAAACCACCGGTTCTC
	CGAACCGTCTGTTCCAGCTGGTTTACCTGCCGCTGAACCCGTCTGACCCGGAAAAAAAAA
	ACTCTAACAACTTCCAGGAACGTCTGGACACCCCGACCGGTCTGTCTCGTCGTTTCCTGG
	ACCTGACCCTGGACGCGTTCGCGGGTAAACTGCTGACCGACCCGGTTACCCAGGAACTGA
	AAACCATGGCGGGTTTCTACGACCACCTGTTCGGTTTCAAACTGCCGTGCAAACTGGCGG
	CGATGTCTAACCACCCGGGTTCTTCTTCTAAAATGGTTGTTCTGGCGAAACCGAAAAAAG
	GTGTTGCGTCTAACATCGGTTTCGAACCGATCCCGGACCCGGCGCACCCGGTTTTCCGTG
	TTCGTTCTTCTTGGCCGGAACTGAAATACCTGGAAGGTCTGCTGTACCTGCCGGAAGACA
	CCCCGCTGACCATCGAACTGGCGGAAACCTCTGTTTCTTGCCAGTCTGTTTCTTCTGTTG
	CGTTCGACCTGAAAAACCTGACCACCATCCTGGGTCGTGTTGGTGAATTCCGTGTTACCG
	CGGACCAGCCGTTCAAACTGACCCCGATCATCCCGGAAAAAGAAGAATCTTTCATCGGTA
	AAACCTACCTGGGTCTGGACGCGGGTGAACGTTCTGGTGTTGGTTTCGCGATCGTTACCG
	TTGACGGTGACGGTTACGAAGTTCAGCGTCTGGGTGTTCACGAAGACACCCAGCTGATGG
	CGCTGCAGCAGGTTGCGTCTAAATCTCTGAAAGAACCGGTTTTCCAGCCGCTGCGTAAAG
	GTACCTTCCGTCAGCAGGAACGTATCCGTAAATCTCTGCGTGGTTGCTACTGGAACTTCT
	ACCACGCGCTGATGATCAAATACCGTGCGAAAGTTGTTCACGAAGAATCTGTTGGTTCTT
	CTGGTCTGGTTGGTCAGTGGCTGCGTGCGTTCCAGAAAGACCTGAAAAAAGCGGACGTTC
	TGCCGAAAAAAGGTGGTAAAAACGGTGTTGACAAAAAAAAACGTGAATCTTCTGCGCAGG
	ACACCCTGTGGGGTGGTGCGTTCTCTAAAAAAGAAGAACAGCAGATCGCGTTCGAAGTTC
	AGGCGGCGGGTTCTTCTCAGTTCTGCCTGAAATGCGGTTGGTGGTTCCAGCTGGGTATGC
	GTGAAGTTAACCGTGTTCAGGAATCTGGTGTTGTTCTGGACTGGAACCGTTCTATCGTTA
	CCTTCCTGATCGAATCTTCTGGTGAAAAAGTTTACGGTTTCTCTCCGCAGCAGCTGGAAA
	AAGGTTTCCGTCCGGACATCGAAACCTTCAAAAAAATGGTTCGTGACTTCATGCGTCCGC
	CGATGTTCGACCGTAAAGGTCGTCCGGCGGCGGCGTACGAACGTTTCGTTCTGGGTCGTC
	GTCACCGTCGTTACCGTTTCGACAAAGTTTTCGAAGAACGTTTCGGTCGTTCTGCGCTGT
	TCATCTGCCCGCGTGTTGGTTGCGGTAACTTCGACCACTCTTCTGAACAGTCTGCGGTTG
	TTCTGGCGCTGATCGGTTACATCGCGGACAAAGAAGGTATGTCTGGTAAAAAACTGGTTT
	ACGTTCGTCTGGCGGAACTGATGGCGGAATGGAAACTGAAAAAACTGGAACGTTCTCGTG
	TTGAAGAACAGTCTTCTGCGCAGTAAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTT
	TATCTGAAATGTAGGGAGACCCTCAGGTTAAATATTCACTCAGGAAGTTATTACTCAGGA
	AGCAAAGAGGATTACA

SEQ	AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID	TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO:	CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
78	TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
	ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
	TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
	GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
	ACCATGCGGAATCTAAACAGATGCAGTGCCGTAAATGCGGTGCGTCTATGAAATACGAAG
	TTATCGGTCTGGGTAAAAAATCTTGCCGTTACATGTGCCCGGACTGCGGTAACCACACCT
	CTGCGCGTAAAATCCAGAACAAAAAAAAACGTGACAAAAAATACGGTTCTGCGTCTAAAG
	CGCAGTCTCAGCGTATCGCGGTTGCGGGTGCGCTGTACCCGGACAAAAAAGTTCAGACCA
	TCAAAACCTACAAATACCCGGCGGACCTGAACGGTGAAGTTCACGACTCTGGTGTTGCGG
	AAAAAATCGCGCAGGCGATCCAGGAAGACGAAATCGGTCTGCTGGGTCCGTCTTCTGAAT
	ACGCGTGCTGGATCGCGTCTCAGAAACAGTCTGAACCGTACTCTGTTGTTGACTTCTGGT
	TCGACGCGGTTTGCGCGGGTGGTGTTTTCGCGTACTCTGGTGCGCGTCTGCTGTCTACCG
	TTCTGCAGCTGTCTGGTGAAGAATCTGTTCTGCGTGCGGCGCTGGCGTCTTCTCCGTTCG
	TTGACGACATCAACCTGGCGCAGGCGGAAAAATTCCTGGCGGTTTCTCGTCGTACCGGTC
	AGGACAAACTGGGTAAACGTATCGGTGAATGCTTCGCGGAAGGTCGTCTGGAAGCGCTGG
	GTATCAAAGACCGTATGCGTGAATTCGTTCAGGCGATCGACGTTGCGCAGACCGCGGGTC
	AGCGTTTCGCGGCGAAACTGAAAATCTTCGGTATCTCTCAGATGCCGGAAGCGAAACAGT
	GGAACAACGACTCTGGTCTGACCGTTTGCATCCTGCCGGACTACTACGTTCCGGAAGAAA
	ACCGTGCGGACCAGCTGGTTGTTCTGCTGCGTCGTCTGCGTGAAATCGCGTACTGCATGG
	GTATCGAAGACGAAGCGGGTTTCGAACACCTGGGTATCGACCCGGGTGCGCTGTCTAACT
	TCTCTAACGGTAACCCGAAACGTGGTTTCCTGGGTCGTCTGCTGAACAACGACATCATCG
	CGCTGGCGAACAACATGTCTGCGATGACCCCGTACTGGGAAGGTCGTAAAGGTGAACTGA
	TCGAACGTCTGGCGTGGCTGAAACACCGTGCGGAAGGTCTGTACCTGAAAGAACCGCACT
	TCGGTAACTCTTGGGCGGACCACCGTTCTCGTATCTTCTCTCGTATCGCGGGTTGGCTGT
	CTGGTTGCGCGGGTAAACTGAAAATCGCGAAAGACCAGATCTCTGGTGTTCGTACCGACC
	TGTTCCTGCTGAAACGTCTGCTGGACGCGGTTCCGCAGTCTGCGCCGTCTCCGGACTTCA
	TCGCGTCTATCTCTGCGCTGGACCGTTTCCTGGAAGCGGCGGAATCTTCTCAGGACCCGG
	CGGAACAGGTTCGTGCGCTGTACGCGTTCCACCTGAACGCGCCGGCGGTTCGTTCTATCG
	CGAACAAAGCGGTTCAGCGTTCTGACTCTCAGGAATGGCTGATCAAAGAACTGGACGCGG
	TTGACCACCTGGAATTCAACAAAGCGTTCCCGTTCTTCTCTGACACCGGTAAAAAAAAAA
	AAAAAGGTGCGAACTCTAACGGTGCGCCGTCTGAAGAAGAATACACCGAAACCGAATCTA
	TCCAGCAGCCGGAAGACGCGGAACAGGAAGTTAACGGTCAGGAAGGTAACGGTGCGTCTA
	AAAACCAGAAAAAATTCCAGCGTATCCCGCGTTTCTTCGGTGAAGGTTCTCGTTCTGAAT
	ACCGTATCCTGACCGAAGCGCCGCAGTACTTCGACATGTTCTGCAACAACATGCGTGCGA
	TCTTCATGCAGCTGGAATCTCAGCCGCGTAAAGCGCCGCGTGACTTCAAATGCTTCCTGC
	AGAACCGTCTGCAGAAACTGTACAAACAGACCTTCCTGAACGCGCGTTCTAACAAATGCC
	GTGCGCTGCTGGAATCTGTTCTGATCTCTTGGGGTGAATTCTACACCTACGGTGCGAACG
	AAAAAAAATTCCGTCTGCGTCACGAAGCGTCTGAACGTTCTTCTGACCCGGACTACGTTG
	TTCAGCAGGCGCTGGAAATCGCGCGTCGTCTGTTCCTGTTCGGTTTCGAATGGCGTGACT
	GCTCTGCGGGTGAACGTGTTGACCTGGTTGAAATCCACAAAAAAGCGATCTCTTTCCTGC
	TGGCGATCACCCAGGCGGAAGTTTCTGTTGGTTCTTACAACTGGCTGGGTAACTCTACCG
	TTTCTCGTTACCTGTCTGTTGCGGGTACCGACACCCTGTACGGTACCCAGCTGGAAGAAT
	TCCTGAACGCGACCGTTCTGTCTCAGATGCGTGGTCTGGCGATCCGTCTGTCTTCTCAGG
	AACTGAAAGACGGTTTCGACGTTCAGCTGGAATCTTCTTGCCAGGACAACCTGCAGCACC
	TGCTGGTTTACCGTGCGTCTCGTGACCTGGCGGCGTGCAAACGTGCGACCTGCCCGGCGG
	AACTGGACCCGAAAATCCTGGTTCTGCCGGTTGGTGCGTTCATCGCGTCTGTTATGAAAA
	TGATCGAACGTGGTGACGAACCGCTGGCGGGTGCGTACCTGCGTCACCGTCCGCACTCTT
	TCGGTTGGCAGATCCGTGTTCGTGGTGTTGCGGAAGTTGGTATGGACCAGGGTACCGCGC
	TGGCGTTCCAGAAACCGACCGAATCTGAACCGTTCAAAATCAAACCGTTCTCTGCGCAGT
	ACGGTCCGGTTCTGTGGCTGAACTCTTCTTCTTACTCTCAGTCTCAGTACCTGGACGGTT
	TCCTGTCTCAGCCGAAAAACTGGTCTATGCGTGTTCTGCCGCAGGCGGGTTCTGTTCGTG
	TTGAACAGCGTGTTGCGCTGATCTGGAACCTGCAGGCGGGTAAAATGCGTCTGGAACGTT
	CTGGTGCGCGTGCGTTCTTCATGCCGGTTCCGTTCTCTTTCCGTCCGTCTGGTTCTGGTG
	ACGAAGCGGTTCTGGCGCCGAACCGTTACCTGGGTCTGTTCCCGCACTCTGGTGGTATCG
	AATACGCGGTTGTTGACGTTCTGGACTCTGCGGGTTTCAAAATCCTGGAACGTGGTACCA
	TCGCGGTTAACGGTTTCTCTCAGAAACGTGGTGAACGTCAGGAAGAAGCGCACCGTGAAA
	AACAGCGTCGTGGTATCTCTGACATCGGTCGTAAAAAACCGGTTCAGGCGGAAGTTGACG
	CGGCGAACGAACTGCACCGTAAATACACCGACGTTGCGACCCGTCTGGGTTGCCGTATCG
	TTGTTCAGTGGGCGCCGCAGCCGAAACCGGGTACCGCGCCGACCGCGCAGACCGTTTACG
	CGCGTGCGGTTCGTACCGAAGCGCCGCGTTCTGGTAACCAGGAAGACCACGCGCGTATGA
	AATCTTCTTGGGGTTACACCTGGGGTACCTACTGGGAAAAACGTAAACCGGAAGACATCC
	TGGGTATCTCTACCCAGGTTTACTGGACCGGTGGTATCGGTGAATCTTGCCCGGCGGTTG
	CGGTTGCGCTGCTGGGTCACATCCGTGCGACCTCTACCCAGACCGAATGGGAAAAAGAAG
	AAGTTGTTTTCGGTCGTCTGAAAAAATTCTTCCCGTCTTAAGAAATCATCCTTAGCGAAA
	GCTAAGGATTTTTTTTATCTGAAATGTAGGGAGACCCTCAGGTTAAATATTCACTCAGGA
	AGTTATTACTCAGGAAGCAAAGAGGATTACA

SEQ	AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID	TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO:	CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
79	TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
	ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
	TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
	GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
	ACCATGAAAAACGTATCAACAAAATCCGTAAAAAACTGTCTGCGGACAACGCGACCAAAC
	CGGTTTCTCGTTCTGGTCCGATGAAAACCCTGCTGGTTCGTGTTATGACCGACGACCTGA
	AAAAACGTCTGGAAAAACGTCGTAAAAAACCGGAAGTTATGCCGCAGGTTATCTCTAACA
	ACGCGGCGAACAACCTGCGTATGCTGCTGGACGACTACACCAAAATGAAAGAAGCGATCC
	TGCAGGTTTACTGGCAGGAATTCAAAGACGACCACGTTGGTCTGATGTGCAAATTCGCGC
	AGCCGGCGTCTAAAAAAATCGACCAGAACAAACTGAAACCGGAAATGGACGAAAAAGGTA
	ACCTGACCACCGCGGGTTTCGCGTGCTCTCAGTGCGGTCAGCCGCTGTTCGTTTACAAAC
	TGGAACAGGTTTCTGAAAAAGGTAAAGCGTACACCAACTACTTCGGTCGTTGCAACGTTG
	CGGAACACGAAAAACTGATCCTGCTGGCGCAGCTGAAACCGGAAAAAGACTCTGACGAAG
	CGGTTACCTACTCTCTGGGTAAATTCGGTCAGCGTGCGCTGGACTTCTACTCTATCCACG
	TTACCAAAGAATCTACCCACCCGGTTAAACCGCTGGCGCAGATCGCGGGTAACCGTTACG
	CGTCTGGTCCGGTTGGTAAAGCGCTGTCTGACGCGTGCATGGGTACCATCGCGTCTTTCC
	TGTCTAAATACCAGGACATCATCATCGAACACCAGAAAGTTGTTAAAGGTAACCAGAAAC
	GTCTGGAATCTCTGCGTGAACTGGCGGGTAAAGAAAACCTGGAATACCCGTCTGTTACCC
	TGCCGCCGCAGCCGCACACCAAAGAAGGTGTTGACGCGTACAACGAAGTTATCGCGCGTG
	TTCGTATGTGGGTTAACCTGAACCTGTGGCAGAAACTGAAACTGTCTCGTGACGACGCGA
	AACCGCTGCTGCGTCTGAAAGGTTTCCCGTCTTTCCCGGTTGTTGAACGTCGTGAAAACG
	AAGTTGACTGGTGGAACACCATCAACGAAGTTAAAAAACTGATCGACGCGAAACGTGACA
	TGGGTCGTGTTTTCTGGTCTGGTGTTACCGCGGAAAAACGTAACACCATCCTGGAAGGTT
	ACAACTACCTGCCGAACGAAAACGACCACAAAAAACGTGAAGGTTCTCTGGAAAACCCGA
	AAAAACCGGCGAAACGTCAGTTCGGTGACCTGCTGCTGTACCTGGAAAAAAAATACGCGG
	GTGACTGGGGTAAAGTTTTCGACGAAGCGTGGGAACGTATCGACAAAAAAATCGCGGGTC
	TGACCTCTCACATCGAACGTGAAGAAGCGCGTAACGCGGAAGACGCGCAGTCTAAAGCGG
	TTCTGACCGACTGGCTGCGTGCGAAAGCGTCTTTCGTTCTGGAACGTCTGAAAGAAATGG
	ACGAAAAAGAATTCTACGCGTGCGAAATCCAGCTGCAGAAATGGTACGGTGACCTGCGTG
	GTAACCCGTTCGCGGTTGAAGCGGAAAACCGTGTTGTTGACATCTCTGGTTTCTCTATCG
	GTTCTGACGGTCACTCTATCCAGTACCGTAACCTGCTGGCGTGGAAATACCTGGAAAACG
	GTAAACGTGAATTCTACCTGCTGATGAACTACGGTAAAAAAGGTCGTATCCGTTTCACCG
	ACGGTACCGACATCAAAAAATCTGGTAAATGGCAGGGTCTGCTGTACGGTGGTGGTAAAG
	CGAAAGTTATCGACCTGACCTTCGACCCGGACGACGAACAGCTGATCATCCTGCCGCTGG
	CGTTCGGTACCCGTCAGGGTCGTGAATTCATCTGGAACGACCTGCTGTCTCTGGAAACCG
	GTCTGATCAAACTGGCGAACGGTCGTGTTATCGAAAAAACCATCTACAACAAAAAAATCG
	GTCGTGACGAACCGGCGCTGTTCGTTGCGCTGACCTTCGAACGTCGTGAAGTTGTTGACC
	CGTCTAACATCAAACCGGTTAACCTGATCGGTGTTGACCGTGGTGAAAACATCCCGGCGG
	TTATCGCGCTGACCGACCCGGAAGGTTGCCCGCTGCCGGAATTCAAAGACTCTTCTGGTG
	GTCCGACCGACATCCTGCGTATCGGTGAAGGTTACAAAGAAAAACAGCGTGCGATCCAGG
	CGGCGAAAGAAGTTGAACAGCGTCGTGCGGGTGGTTACTCTCGTAAATTCGCGTCTAAAT
	CTCGTAACCTGGCGGACGACATGGTTCGTAACTCTGCGCGTGACCTGTTCTACCACGCGG
	TTACCCACGACGCGGTTCTGGTTTTCGAAAACCTGTCTCGTGGTTTCGGTCGTCAGGGTA
	AACGTACCTTCATGACCGAACGTCAGTACACCAAAATGGAAGACTGGCTGACCGCGAAAC
	TGGCGTACGAAGGTCTGACCTCTAAAACCTACCTGTCTAAAACCCTGGCGCAGTACACCT
	CTAAAACCTGCTCTAACTGCGGTTTCACCATCACCACCGCGGACTACGACGGTATGCTGG
	TTCGTCTGAAAAAAACCTCTGACGGTTGGGCGACCACCCTGAACAACAAAGAACTGAAAG
	CGGAAGGTCAGATCACCTACTACAACCGTTACAAACGTCAGACCGTTGAAAAAGAACTGT
	CTGCGGAACTGGACCGTCTGTCTGAAGAATCTGGTAACAACGACATCTCTAAATGGACCA
	AAGGTCGTCGTGACGAAGCGCTGTTCCTGCTGAAAAAACGTTTCTCTCACCGTCCGGTTC
	AGGAACAGTTCGTTTGCCTGGACTGCGGTCACGAAGTTCACGCGGACGAACAGGCGGCGC
	TGAACATCGCGCGTTCTTGGCTGTTCCTGAACTCTAACTCTACCGAATTCAAATCTTACA
	AATCTGGTAAACAGCCGTTCGTTGGTGCGTGGCAGGCGTTCTACAAACGTCGTCTGAAAG
	AAGTTTGGAAACCGAACGCGTAAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAT
	CTGAAATGTAGGGAGACCCTCAGGTTAAATATTCACTCAGGAAGTTATTACTCAGGAAGC
	AAAGAGGATTACA

SEQ	AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTGCCG
ID	TCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAG
NO:	CATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAA
80	TCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC
	ATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGT
	TTCTCCATACCCGTTTTTTTGGGCTAGCAGTAATACGACTCACTATAGGGGTCTCATCTC
	GTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCATGCACCATCATCATC
	ACCATAAACGTATCAACAAAATCCGTCGTCGTCTGGTTAAAGACTCTAACACCAAAAAAG
	CGGGTAAAACCGGTCCGATGAAAACCCTGCTGGTTCGTGTTATGACCCCGGACCTGCGTG
	AACGTCTGGAAAACCTGCGTAAAAAACCGGAAAACATCCCGCAGCCGATCTCTAACACCT
	CTCGTGCGAACCTGAACAAACTGCTGACCGACTACACCGAAATGAAAAAAGCGATCCTGC
	ACGTTTACTGGGAAGAATTCCAGAAAGACCCGGTTGGTCTGATGTCTCGTGTTGCGCAGC
	CGGCGCCGAAAAACATCGACCAGCGTAAACTGATCCCGGTTAAAGACGGTAACGAACGTC
	TGACCTCTTCTGGTTTCGCGTGCTCTCAGTGCTGCCAGCCGCTGTACGTTTACAAACTGG
	AACAGGTTAACGACAAAGGTAAACCGCACACCAACTACTTCGGTCGTTGCAACGTTTCTG
	AACACGAACGTCTGATCCTGCTGTCTCCGCACAAACCGGAAGCGAACGACGAACTGGTTA
	CCTACTCTCTGGGTAAATTCGGTCAGCGTGCGCTGGACTTCTACTCTATCCACGTTACCC
	GTGAATCTAACCACCCGGTTAAACCGCTGGAACAGATCGGTGGTAACTCTTGCGCGTCTG
	GTCCGGTTGGTAAAGCGCTGTCTGACGCGTGCATGGGTGCGGTTGCGTCTTTCCTGACCA
	AATACCAGGACATCATCCTGGAACACCAGAAAGTTATCAAAAAAAACGAAAAACGTCTGG
	CGAACCTGAAAGACATCGCGTCTGCGAACGGTCTGGCGTTCCCGAAAATCACCCTGCCGC
	CGCAGCCGCACACCAAAGAAGGTATCGAAGCGTACAACAACGTTGTTGCGCAGATCGTTA
	TCTGGGTTAACCTGAACCTGTGGCAGAAACTGAAAATCGGTCGTGACGAAGCGAAACCGC
	TGCAGCGTCTGAAAGGTTTCCCGTCTTTCCCGCTGGTTGAACGTCAGGCGAACGAAGTTG
	ACTGGTGGGACATGGTTTGCAACGTTAAAAAACTGATCAACGAAAAAAAAGAAGACGGTA
	AAGTTTTCTGGCAGAACCTGGCGGGTTACAAACGTCAGGAAGCGCTGCTGCCGTACCTGT
	CTTCTGAAGAAGACCGTAAAAAAGGTAAAAAATTCGCGCGTTACCAGTTCGGTGACCTGC
	TGCTGCACCTGGAAAAAAAACACGGTGAAGACTGGGGTAAAGTTTACGACGAAGCGTGGG
	AACGTATCGACAAAAAAGTTGAAGGTCTGTCTAAACACATCAAACTGGAAGAAGAACGTC
	GTTCTGAAGACGCGCAGTCTAAAGCGGCGCTGACCGACTGGCTGCGTGCGAAAGCGTCTT
	TCGTTATCGAAGGTCTGAAAGAAGCGGACAAAGACGAATTCTGCCGTTGCGAACTGAAAC
	TGCAGAAATGGTACGGTGACCTGCGTGGTAAACCGTTCGCGATCGAAGCGGAAAACTCTA
	TCCTGGACATCTCTGGTTTCTCTAAACAGTACAACTGCGCGTTCATCTGGCAGAAAGACG
	GTGTTAAAAAACTGAACCTGTACCTGATCATCAACTACTTCAAAGGTGGTAAACTGCGTT
	TCAAAAAAATCAAACCGGAAGCGTTCGAAGCGAACCGTTTCTACACCGTTATCAACAAAA
	AATCTGGTGAAATCGTTCCGATGGAAGTTAACTTCAACTTCGACGACCCGAACCTGATCA
	TCCTGCCGCTGGCGTTCGGTAAACGTCAGGGTCGTGAATTCATCTGGAACGACCTGCTGT
	CTCTGGAAACCGGTTCTCTGAAACTGGCGAACGGTCGTGTTATCGAAAAAACCCTGTACA
	ACCGTCGTACCCGTCAGGACGAACCGGCGCTGTTCGTTGCGCTGACCTTCGAACGTCGTG
	AAGTTCTGGACTCTTCTAACATCAAACCGATGAACCTGATCGGTATCGACCGTGGTGAAA
	ACATCCCGGCGGTTATCGCGCTGACCGACCCGGAAGGTTGCCCGCTGTCTCGTTTCAAAG
	ACTCTCTGGGTAACCCGACCCACATCCTGCGTATCGGTGAATCTTACAAAGAAAAACAGC
	GTACCATCCAGGCGGCGAAAGAAGTTGAACAGCGTCGTGCGGGTGGTTACTCTCGTAAAT
	ACGCGTCTAAAGCGAAAAACCTGGCGGACGACATGGTTCGTAACACCGCGCGTGACCTGC
	TGTACTACGCGGTTACCCAGGACGCGATGCTGATCTTCGAAAACCTGTCTCGTGGTTTCG
	GTCGTCAGGGTAAACGTACCTTCATGGCGGAACGTCAGTACACCCGTATGGAAGACTGGC
	TGACCGCGAAACTGGCGTACGAAGGTCTGCCGTCTAAAACCTACCTGTCTAAAACCCTGG
	CGCAGTACACCTCTAAAACCTGCTCTAACTGCGGTTTCACCATCACCTCTGCGGACTACG
	ACCGTGTTCTGGAAAAACTGAAAAAAACCGCGACCGGTTGGATGACCACCATCAACGGTA
	AAGAACTGAAAGTTGAAGGTCAGATCACCTACTACAACCGTTACAAACGTCAGAACGTTG
	TTAAAGACCTGTCTGTTGAACTGGACCGTCTGTCTGAAGAATCTGTTAACAACGACATCT
	CTTCTTGGACCAAAGGTCGTTCTGGTGAAGCGCTGTCTCTGCTGAAAAAACGTTTCTCTC
	ACCGTCCGGTTCAGGAAAAATTCGTTTGCCTGAACTGCGGTTTCGAAACCCACGCGGACG
	AACAGGCGGCGCTGAACATCGCGCGTTCTTGGCTGTTCCTGCGTTCTCAGGAATACAAAA
	AATACCAGACCAACAAAACCACCGGTAACACCGACAAACGTGCGTTCGTTGAAACCTGGC
	AGTCTTTCTACCGTAAAAAACTGAAAGAAGTTTGGAAACCGGAAATCATCCTTAGCGAAA
	GCTAAGGATTTTTTTTATCTGAAATGTAGGGAGACCCTCAGGTTAAATATTCACTCAGGA
	AGTTATTACTCAGGAAGCAAAGAGGATTACA

SEQ	tgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttatt
ID	aaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtc
NO:	tataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgcc
81	atagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctct
	actgtttctccatacccgtttttttgggctagcaccgcctatctcgtgtgagataggcgg
	agatacgaactttaagAAGGAGatatacc

SEQ	TGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATT
ID	AAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTC
NO:	TATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCC
82	ATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCT
	ACTGTTTCTCCATACCCGTTTTTTTGGGTAGCGGATCCTACCTGAC

SEQ	AATCAGGAGAGCGTTTTCAATCCTACCTCTGGCGCAGTTGATATGTCAAACAGGTTTCTA
ID	GAGCACAGCTAACACCACGTCGTCCCTATCTGCTGCCCTAGGTCTATGAGTGGTTGCTGG
NO:	ATAACTTTACGGGCATGCATAAGGCTCGTAATATATATTCAGGGAGACCACAACGGTTTC
83	CCTCTACAAATAATTTTGTTTAACTTTTACTAGAGCTAGCAGTAATACGACTCACTATAG
	GGGTCTCATCTCGTGTGAGATAGGCGGAGATACGAACTTTAAGAGGAGGATATACCA

SEQ	GTTTGAGAGATATGTAAATTCAAAGGATAATCAAAC
ID
NO:
84

SEQ	actacattttttaagacctaattttgagt
ID
NO:
85

SEQ	ctcaaaactcattcgaatctctactctttgtagat
ID
NO:
86

SEQ	CTCTAGCAGGCCTGGCAAATTTCTACTGTTGTAGAT
ID
NO:
87

SEQ	CCGTCTAAAACTCATTCAGAATTTCTACTAGTGTAGAT
ID
NO:
88

SEQ	GTCTAGGTACTCTCTTTAATTTCTACTATTGT
ID
NO:
89

SEQ	gttaagttatatagaataatttctactgttgtaga
ID
NO:
90

SEQ	gtttaaaaccactttaaaatttctactattgta
ID
NO:
91

SEQ	GTTTGAGAATGATGTAAAAATGTATGGTACACAGAAATGTTTTAATACCATATTTTTACA
ID	TCACTCTCAAACATACATCTCTTGTTACTGTTTATCGTATCCAGATTAAATTTCACGTTT
NO:	TT
92

SEQ	CTCTACAACTGATAAAGAATTTCTACTTTTGTAGAT
ID
NO:93

SEQ	GTCTGGCCCCAAATTTTAATTTCTACTGTTGTAGAT
ID
NO:
94

SEQ	GTCAAAAGACCTTTTTAATTTCTACTCTTGTAGAT
ID
NO:
95

SEQ	GTCTAGAGGACAGAATTTTTCAACGGGTGTGCCAATGGCCACTTTCCAGGTGGCAAAGCC
ID	CGTTGAGCTTCTACGGAAGTGGCAC
NO:
96

SEQ	CGAGGTTCTGTCTTTTGGTCAGGACAACCGTCTAGCTATAAGTGCTGCAGGGGTGTGAGA
ID	AACTCCTATTGCTGGACGATGTCTCTTTTAACGAGGCATTAGCAC
NO:
97

SEQ	GAACGAGGGACGTTTTGTCTCCAATGATTTTGCTATGACGACCTCGAACTGTGCCTTCAA
ID	GTCTGAGGCGAAAAAGAAATGGAAAAAAGTGTCTCATCGCTCTACCTCGTAGTTAGAGG
NO:
98

SEQ	AATTACTGATGTTGTGATGAAGG
ID
NO:
99

SEQ	TATACCATAAGGATTTAAAGACT
ID
NO:
100

SEQ	GTCTTTACTCTCACCTTTCCACCTG
ID
NO:
101

SEQ	ATTTGAAGGTATCTCCGATAAGTAAAACGCATCAAAG
ID
NO:
102

SEQ	GTTTGAAGATATCTCCGATAAATAAGAAGCATCAAAG
ID
NO:
103

SEQ	TTGTTTTAATACCATATTTTTACATCACTCTCAAAC
ID
NO:
104

SEQ	AAAGAACGCTCGCTCAGTGTTCTGACCTTTCGAGCGCCTGTTCAGGGCGAAAACCCTGGG
ID	AGGCGCTCGAATCATAGGTGGGACAAGGGATTCGCGGCGAAAA
NO:
105

SEQ	GTTTGAGAATGATGTAAAAATGTATGGTACACAGAAATGTTTTAATACCATATTTTTACA
ID	TCACTCTCAAACATACATCTCTTGTTACTGTTTATCGTATCCAGATTAAATTTCACGTTT
NO:	TT
106

SEQ	GTCTAGAGGACAGAATTTTTCAACGGGTGTGCCAATGGCCACTTTCCAGGTGGCAAAGCC
ID	CGTTGAGCTTCTACGGAAGTGGCAC
NO:
107

SEQ	MSIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAKDYKKAKQIIDKYHQF
ID	FIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAKDTIKKQISEYIKDSEKFK
NO:	NLFNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDIDEALEIIKSFKGWTTYFK
108	GFHENRKNVYSSNDIPTSIIYRIVDDNLPKFLENKAKYESLKDKAPEAINYEQIKKDLAE
	ELTFDIDYKTSEVNQRVFSLDEVFEIANFNNYLNQSGITKFNTIIGGKFVNGENTKRKGI
	NEYINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVIDKLEDDSDVVTTMQSFYEQIA
	AFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSLTDLSQQVFDDYSVIGTAVLEY
	ITQQIAPKNLDNPSKKEQELIAKKTEKAKYLSLETIKLALEEFNKHRDIDKQCRFEEILA
	NFAAIPMIFDEIAQNKDNLAQISIKYQNQGKKDLLQASAEDDVKAIKDLLDQTNNLLHKL
	KIFHISQSEDKANILDKDEHFYLVFEECYFELANIVPLYNKIRNYITQKPYSDEKFKLNF
	ENSTLANGWDKNKEPDNTAILFIKDDKYYLGVMNKKNNKIFDDKAIKENKGEGYKKIVYK
	LLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNHSTHTKNGSPQKGYEKFEFNIEDCRKF
	IDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEFYREVENQGYKLTFENISESYIDSVVNQ
	GKLYLFQIYNKDFSAYSKGRPNLHTLYWKALFDERNLQDVVYKLNGEAELFYRKQSIPKK
	ITHPAKEAIANKNKDNPKKESVFEYDLIKDKRFTEDKFFFHCPITINFKSSGANKFNDEI
	NLLLKEKANDVHILSIDRGERHLAYYTLVDGKGNIIKQDTFNIIGNDRMKTNYHDKLAAI
	EKDRDSARKDWKKINNIKEMKEGYLSQVVHEIAKLVIEYNAIVVFEDLNFGFKRGRFKVE
	KQVYQKLEKMLIEKLNYLVFKDNEFDKTGGVLRAYQLTAPFETFKKMGKQTGIIYYVPAG
	FTSKICPVTGFVNQLYPKYESVSKSQEFFSKFDKICYNLDKGYFEFSFDYKNFGDKAAKG
	KWTIASFGSRLINFRNSDKNHNWDTREVYPTKELEKLLKDYSIEYGHGECIKAAICGESD
	KKFFAKLTSVLNTILQMRNSKTGTELDYLISPVADVNGNFFDSRQAPKNMPQDADANGAY
	HIGLKGLMLLGRIKNNQEGKKLNLVIKNEEYFEFVQNRNN

SEQ	MSIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAKDYKKAKQIIDKYHQF
ID	FIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAKDTIKKQISEYIKDSEKFK
NO:	NLFNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDIDEALEIIKSFKGWTTYFK
109	GFHENRKNVYSSDDIPTSIIYRIVDDNLPKFLENKAKYESLKDKAPEAINYEQIKKDLAE
	ELTFDIDYKTSEVNQRVFSLDEVFEIANFNNYLNQSGITKFNTIIGGKFVNGENTKRKGI
	NEYINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVIDKLEDDSDVVTTMQSFYEQIA
	AFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSLTDLSQQVFDDYSVIGTAVLEY
	ITQQVAPKNLDNPSKKEQDLIAKKTEKAKYLSLETIKLALEEFNKHRDIDKQCRFEEILA
	NFAAIPMIFDEIAQNKDNLAQISLKYQNQGKKDLLQASAEEDVKAIKDLLDQTNNLLHRL
	KIFHISQSEDKANILDKDEHFYLVFEECYFELANIVPLYNKIRNYITQKPYSDEKFKLNF
	ENSTLANGWDKNKEPDNTAILFIKDDKYYLGVMNKKNNKIFDDKAIKENKGEGYKKIVYK
	LLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNHSTHTKNGNPQKGYEKFEFNIEDCRKF
	IDFYKESISKHPEWKDFGFRFSDTQRYNSIDEFYREVENQGYKLTFENISESYIDSVVNQ
	GKLYLFQIYNKDFSAYSKGRPNLHTLYWKALFDERNLQDVVYKLNGEAELFYRKQSIPKK
	ITHPAKEAIANKNKDNPKKESVFEYDLIKDKRFTEDKFFFHCPITINFKSSGANKFNDEI
	NLLLKEKANDVHILSIDRGERHLAYYTLVDGKGNIIKQDTFNIIGNDRMKTNYHDKLAAI
	EKDRDSARKDWKKINNIKEMKEGYLSQVVHEIAKLVIEHNAIVVFEDLNFGFKRGRFKVE
	KQVYQKLEKMLIEKLNYLVFKDNEFDKTGGVLRAYQLTAPFETFKKMGKQTGIIYYVPAG
	FTSKICPVTGFVNQLYPKYESVSKSQEFFSKFDKICYNLDKGYFEFSFDYKNFGDKAAKG
	KWTIASFGSRLINFRNSDKNHNWDTREVYPTKELEKLLKDYSIEYGHGECIKAAICGESD
	KKFFAKLTSVLNTILQMRNSKTGTELDYLISPVADVNGNFFDSRQAPKNMPQDADANGAY
	HIGLKGLMLLDRIKNNQEGKKLNLVIKNEEYFEFVQNRNN

SEQ	MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAE
ID	ATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFG
NO:	NIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSD
110	VDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGN
	LIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAI
	LLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYA
	GYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELH
	AILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEE
	VVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFL
	SGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKI
	IKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWG
	RLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSL
	HEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRER
	MKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDH
	IVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNL
	TKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKS
	KLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRK
	MIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDF
	ATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVA
	YSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPK
	YSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVE
	QHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
	PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD

SEQ	PKKKRKV
ID
NO:
111

SEQ	KRPAATKKAGQAKKKK
ID
NO:
112

SEQ	PAAKRVKLD
ID
NO:
113

SEQ	RQRRNELKRSP
ID
NO:
114

SEQ	NQSSNFGPMKGGNFGGRSSGPYGGGGQYFAKPRNQGGY
ID
NO:
115

SEQ	RMRIZFKNKGKDTAELRRRRVEVSVELRKAKKDEQILKRRNV
ID
NO:
116

SEQ	VSRKRPRP
ID
NO:
117

SEQ	PPKKARED
ID
NO:
118

SEQ	PQPKKKPL
ID
NO:
119

SEQ	SALIKKKKKMAP
ID
NO:
120

SEQ	DRLRR
ID
NO:
121

SEQ	PKQKKRK
ID
NO:
122

SEQ	RKLKKKIKKL
ID
NO:
123

SEQ	REKKKFLKRR
ID
NO:
124

SEQ	KRKGDEVDGVDEVAKKKSKK
ID
NO:
125

SEQ	RKCLQAGMNLEARKTKK
ID
NO:
126

SEQ	ATGGGTAAGATGTATTATCTGGGTTTGGATATAGGCACTAACTCTGTGGGATATGCAGTA
ID	ACTGATCCCTCGTATCACTTGTTAAAGTTCAAAGGCGAACCCATGTGGGGAGCACATGTA
NO:	TTTGCTGCGGGTAATCAGAGTGCCGAAAGGCGATCTTTCAGAACATCCAGGAGGCGATTA
127	GATAGGAGACAGCAAAGAGTAAAGCTTGTGCAAGAGATCTTTGCTCCTGTCATTTCACCT
	ATAGACCCTCGTTTTTTTATAAGATTGCACGAATCGGCTCTATGGAGAGACGATGTTGCC
	GAAACAGATAAACATATCTTTTTCAATGATCCCACTTATACAGACAAGGAATACTACTCC
	GACTACCCGACAATTCATCATTTGATCGTCGATCTTATGGAGAGCTCTGAAAAGCATGAC
	CCCCGACTTGTCTATTTGGCTGTAGCTTGGTTAGTTGCTCATAGAGGTCATTTCTTGAAT
	GAAGTAGATAAAGACAATATAGGTGATGTACTTTCTTTTGATGCTTTCTACCCGGAATTT
	TTGGCCTTTTTGTCAGACAATGGCGTCAGTCCCTGGGTCTGTGAGTCGAAGGCCCTTCAA
	GCTACTCTGCTGTCTAGGAATAGCGTGAACGACAAATATAAAGCATTAAAATCGCTGATA
	TTCGGATCGCAAAAACCGGAAGATAACTTTGACGCTAACATCTCTGAAGATGGTTTAATC
	CAATTGCTGGCGGGTAAGAAAGTTAAAGTAAACAAACTATTCCCACAAGAGTCCAACGAT
	GCTAGCTTTACGTTGAATGATAAAGAAGACGCTATTGAAGAAATTCTAGGTACTTTAACG
	CCTGACGAGTGCGAATGGATCGCTCATATTCGCAGATTGTTCGATTGGGCCATCATGAAA
	CACGCGCTAAAGGATGGCAGGACGATATCTGAATGAAAAGTGAAGCTATACGAGCAGCAT
	CATCATGACTTGACTCAGTTAAAGTACTTTGTGAAGACCTACCTAGCTAAAGAGTATGAT
	GATATCTTCAGAAACGTAGACTCCGAGACAACTAAAAATTATGTAGCTTATTCTTACCAT
	GTGAAGGAAGTGAAAGGCACATTACCAAAAAATAAAGCAACGCAAGAAGAATTTTGTAAA
	TACGTCCTTGGCAAAGTCAAAAACATTGAATGTTCCGAAGCAGACAAGGTTGATTttGAt
	GAAAtGAtAcAAcGActtAcGGAcAAttcttttAtGccAAAGcAAGtctcAGGtGAAAAT
	AGAGTAATACCATACCAGTTGTACTACTATGAATTAAAGACAATTTTAAACAAAGCCGCC
	TCATATCTACCTTTTTTGACACAATGCGGTAAAGATGCTATTTCTAACCAAGACAAATTA
	CTGTCTATAATGACATTTCGCATACCATATTTCGTCGGCCCTTTAAGGAAAGATAATTCA
	GAACATGCCTGGTTGGAACGTAAAGCGGGTAAAATTTACCCGTGGAACTTTAATGATAAA
	GTAGATCTTGATAAATCGGAGGAAGCCTTTATCCGTAGGATGACCAATACTTGCACGTAT
	TACCCAGGAGAAGACGTGTTACCATTAGATTCACTTATCTATGAAAAGTTTATGATCTTG
	AATGAGATAAACAATATTAGGATTGACGGATACCCCATTTCTGTTGATGTGAAACAACAA
	GTATTTGGTTTATTTGAGAAGAAAAGGCGAGTAACAGTTAAGGATATTCAAAATCTACTA
	TTATCTCTTGGAGCGTTGGATAAACACGGTAAGCTGACTGGTATTGACACGACAATACAC
	TCTAATTATAACACTTATCATCATTTTAAATCTCTTATGGAGCGGGGAGTATTGACCAGA
	GATGATGTGGAAAGAATAGTGGAAAGAATGACATATTCTGACGATACTAAGAGGGTCAGA
	CTGTGGTTAAATAATAATTATGGAACTCTAACAGCTGACGATGTTAAGCATATCTCAAGA
	CTCAGAAAACACGATTTCGGCCGTTTGTCTAAAATGTTTTTGACAGGATTGAAAGGTGTT
	CATAAGGAGACAGGCGAGAGAGCAAGTATACTGGATTTTATGTGGAATACTAACGACAAT
	TTAATGCAACTACTGTCCGAATGTTACACATTCTCGGATGAGATCACCAAATTACAAGAG
	GCCTACTACGCAAAAGCTCAATTATCGCTAAATGACTTCTTGGACTCTATGTATATATCA
	AACGCCGTTAAGAGACCTATTTATCGGACCTTAGCGGTAGTAAATGATATTAGAAAGGCA
	TGCGGGACGGCACCTAAAAGAATTTTCATCGAGATGGCGCGAGATGGAGAGTCTAAGAAG
	AAAAGATCTGTGACTCGTAGAGAGCAAATTAAAAATCTCTATAGATCAATTCGTAAAGAC
	TTTCAACAAGAAGTTGATTTTCTGGAAAAGATATTGGAAAATAAGAGTGACGGGCAGCTT
	CAGTCTGACGCTTTATATTTGTATTTTGCTCAATTAGGCAGAGACATGTACACAGGTGAT
	CCAATCAAATTAGAACATATTAAAGACCAATCTTTTTACAACATTGATCATATTTATCCT
	CAATCGATGGTGAAAGATGACAGTTTGGATAACAAGGTACTAGTCCAAAGCGAAATCAAT
	GGCGAAAAGAGTTCGCGCTATCCATTAGACGCAGCCATTAGAAACAAAATGAAGCCGTTG
	TGGGATGCCTACTATAATCATGGATTAATTTCTCTTAAGAAATACCAGCGTTTGACGAGA
	TCTACTCCATTTACGGACGACGAGAAGTGGGATTTTATCAATCGTCAGCTAGTTGAAACT
	AGGCAATCTACTAAAGCTTTAGCAATATTGTTAAAGCGTAAGTTTCCAGATACTGAAATA
	GTTTACTCAAAGGCTGGACTATCCAGCGATTTTAGACATGAATTCGGCCTGGTTAAGAGT
	AGGAATATTAATGATCTACACCATGCTAAAGATGCCTTTCTCGCAATAGTTACTGGGAAC
	GTTTATCATGAAAGATTTAATAGAAGATGGTTTATGGTTAACCAGCCATACTCTGTGAAA
	ACTAAGACATTGTTTACCCATTCAATTAAGAATGGCAACTTTGTCGCTTGGAATGGAGAA
	GAAGATCTTGGACGTATCGTAAAGATGTTGAAACAAAACAAGAACACAATCCACTTCACC
	AGGTTTTCCTTTGATAGGAAGGAGGGATTGTTCGATATTCAACCTCTCAAAGCTTCTACC
	GGATTGGTTCCACGAAAAGCAGGGTTGGATGTTGTTAAATATGGAGGATACGATAAAAGC
	ACTGCCGCGTATTATTTATTAGTACGTTTTACACTCGAGGATAAGAAGACTCAACACAAA
	TTGATGATGATTCCTGTTGAAGGTCTCTACAAAGCACGTATTGACCATGATAAAGAGTTT
	TTAACAGATTATGCTCAGACCACGATCAGCGAAATTCTTCAAAAGGACAAGCAGAAAGTG
	ATCAACATCATGTTCCCTATGGGCACGAGACATATCAAACTGAATTCGATGATTTCTATT
	GATGGATTCTATCTTTCTATTGGTGGGAAGAGTAGCAAAGGTAAGTCAGTACTATGTCAT
	GCTATGGTGCCATTAATCGTCCCACACAAGATAGAATGTTATATCAAGGCTATGGAATCG
	TTTGCAAGAAAATTCAAAGAAAATAATAAATTGAGGATCGTTGAAAAGTTTGATAAAATA
	ACTGTTGAAGATAACTTGAACTTATACGAGCTTTTTCTACAAAAGTTGCAACATAACCCA
	TATAATAAATTTTTCTCTACACAATTTGATGTGTTGACGAACGGTAGAAGTACATTCACC
	AAATTGTCTCCAGAGGAGCAAGTCCAGACTTTACTTAATATACTGAGTATATTTAAAACT
	TGTCGTTCTTCTGGGTGTGATTTAAAATCAATAAATGGTTCCGCTCAAGCGGCTAGAATT
	ATGATATCCGCTGATTTAACTGGCTTATCAAAAAAGTATTCAGATATTAGATTAGTTGAG
	CAAAGCGCATCAGGTCTATTTGTTTCAAAATCTCAAAATCTCTTGGAATACTTGCCAAAA
	AAGAAAAGGAAAGTTTAG

SEQ	ATGAGTAGTTTAACAAAGTTTACCAATAAATATAGTAAGCAACTAACTATAAAGAACGAA
ID	TTGATACCGGTCGGTAAGACTTTGGAAAACATAAAAGAAAATGGGTTGATTGATGGAGAC
NO:	GAGCAATTGAATGAGAATTATCAAAAAGCAAAGATAATAGTAGATGATTTTTTGAGAGAC
128	TTTATTAATAAAGCTCTAAATAACACTCAAATTGGTAACTGGAGAGAGCTAGCCGACGCC
	TTGAACAAGGAAGATGAGGATAATATTGAGAAATTACAAGATAAGATTAGAGGGATTATC
	GTGTCTAAGTTTGAGACTTTTGATCTGTTCAGTTCGTATTCGATTAAAAAGGACGAGAAA
	ATCATCGATGATGATAACGATGTGGAAGAAGAGGAGCTAGACCTTGGGAAGAAGACATCT
	AGCTTCAAATACATATTCAAGAAAAATTTGTTCAAACTTGTCCTTCCTTCATATTTAAAA
	ACAACAAATCAAGATAAGTTAAAAATCATTTCTTCCTTCGATAATTTTAGTACTTATTTT
	CGTGGTTTTTTCGAAAACAGGAAAAATATATTCACTAAAAAGCCTATATCTACCTCTATA
	GCTTATAGAATTGTTCACGATAATTTCCCAAAATTTCTAGATAATATCAGGTGTTTTAAT
	GTTTGGCAAACCGAGTGTCCTCAGTTAATAGTCAAGGCCGACAACTACCTTAAAAGCAAG
	AATGTGATTGCAAAAGATAAGTCTTTGGCTAACTATTTTACAGTCGGTGCCTATGATTAT
	TTTCTGAGTCAAAATGGTATCGATTTCTATAACAACATTATTGGCGGCTTACCAGCTTTT
	GCCGGGCATGAGAAGATTCAGGGTTTGAACGAATTTATCAATCAAGAATGTCAAAAGGAT
	TCTGAATTAAAGTCTAAGCTCAAGAATAGGCACGCTTTCAAAATGGCAGTCTTATTCAAA
	CAAATCCTTTCAGACAGAGAAAAGTCATTTGTGATTGACGAGTTCGAATCAGACGCTCAG
	GTAATTGATGCTGTTAAAAATTTTTACGCGGAACAATGCAAAGATAATAACGTCATATTT
	AATTTATTGAATCTGATCAAGAATATTGCTTTTTTGTCGGATGATGAGTTAGACGGCATT
	TTCATAGAGGGTAAATACCTGTCCTCTGTGTCTCAAAAATTGTATAGTGATTGGTCAAAG
	TTGAGAAATGATATTGAAGATTCGGCTAATTCTAAACAGGGTAACAAAGAATTAGCGAAG
	AAAATCAAAACTAACAAGGGTGATGTTGAAAAGGCTATAAGTAAGTACGAGTTCAGTTTA
	TCTGAACTAAATTCAATTGTTCATGATAACACAAAATTTTCCGATCTTTTATCATGCACA
	TTACATAAAGTTGCAAGTGAAAAATTAGTCAAAGTAAACGAAGGTGATTGGCCAAAACAT
	CTAAAAAACAACGAGGAAAAACAGAAGATAAAAGAACCTCTTGACGCTTTATTGGAAATA
	TACAATACTCTATTAATATTTAACTGTAAAAGTTTTAACAAAAATGGTAATTTCTATGTC
	GACTACGATCGCTGCATTAATGAGTTGTCCAGTGTTGTGTACTTGTATAATAAAACTCGT
	AATTATTGTACGAAAAAGCCGTACAACACTGACAAATTTAAGTTGAATTTCAACTCCCCA
	CAACTGGGTGAGGGCTTCTCTAAAAGTAAAGAGAATGATTGCCTTACATTATTATTTAAA
	AAAGATGATAATTATTATGTCGGAATCATAAGAAAGGGGGCAAAGATCAACTTCGATGAC
	ACTCAGGCCATAGCAGACAACACAGATAACTGTATATTCAAAATGAATTATTTTTTGCTG
	AAGGATGCTAAAAAATTTATCCCCAAATGTTCAATACAATTAAAAGAGGTTAAGGCCCAT
	TTCAAAAAGTCGGAAGATGACTATATTTTGTCCGATAAGGAAAAATTCGCTAGTCCGCTT
	GTTATTAAAAAATCCACATTTCTTCTCGCTACGGCTCATGTGAAAGGAAAGAAGGGCAAT
	ATTAAGAAATTTCAGAAAGAATACTCCAAAGAAAATCCTACGGAGTATAGAAATAGTCTG
	AACGAATGGATAGCATTCTGCAAAGAGTTCTTGAAGACCTATAAAGCTGCCACCATCTTT
	GATATTACAACTTTGAAAAAGGCCGAGGAATACGCTGACATTGTGGAATTCTATAAGGAT
	GTAGATAATCTTTGTTACAAGTTAGAATTTTGCCCTATCAAAACTTCTTTTATCGAAAAT
	CTTATAGATAATGGCGATTTATACCTGTTTAGAATTAATAACAAGGACTTTTCTTCAAAA
	AGTACAGGCACGAAAAACTTACACACATTATACTTGCAGGCTATATTTGACGAGCGAAAC
	TTAAACAACCCCACGATAATGTTGAATGGAGGTGCAGAGTTATTCTACAGAAAAGAATCT
	ATAGAACAGAAAAATCGGATCACGCACAAAGCCGGTAGTATCTTAGTGAATAAAGTGTGC
	AAAGATGGTACAAGTCTAGATGACAAAATCCGTAACGAAATTTACCAGTATGAAAACAAA
	TTCATTGATACTCTTTCGGACGAAGCTAAAAAGGTTCTGCCAAACGTTATTAAGAAAGAG
	GCTACGCATGATATAACAAAAGATAAACGTTTCACTAGCGACAAATTCTTCTTTCATTGT
	CCTTTAACAATCAACTACAAGGAAGGTGACACCAAACAATTTAATAATGAAGTGCTCTCA
	TTCCTTAGAGGTAACCCCGATATCAATATTATCGGCATTGATAGAGGAGAAAGAAACCTA
	ATCTATGTAACAGTCATTAACCAAAAAGGCGAAATATTGGATAGCGTCTCCTTCAATACT
	GTCACCAATAAGTCATCGAAGATAGAACAAACTGTTGATTACGAAGAAAAATTGGCCGTT
	AGAGAAAAGGAACGTATCGAAGCGAAGAGATCTTGGGATAGCATATCCAAGATTGCCACC
	TTGAAGGAGGGTTATCTAAGCGCGATCGTACATGAAATCTGCTTATTAATGATTAAGCAT
	AATGCTATTGTCGTGTTAGAAAACCTGAATGCCGGTTTTAAAAGGATTAGAGGTGGTTTG
	TCAGAAAAGTCAGTATATCAAAAGTTTGAAAAGATGCTTATTAATAAACTCAACTACTTC
	GTTAGCAAGAAAGAAAGTGATTGGAATAAACCGTCAGGTTTGCTCAATGGTCTTCAGTTA
	AGTGATCAATTTGAGTCTTTCGAAAAATTAGGAATTCAAAGTGGATTCATTTTTTATGTA
	CCAGCCGCGTACACTTCAAAAATTGACCCTACGACCGGATTTGCCAACGTCTTGAATTTG
	TCCAAGGTCAGAAATGTTGACGCCATCAAAAGTTTTTTTAGCAACTTCAATGAAATCTCT
	TATTCCAAAAAGGAAGCCCTTTTCAAGTTTTCTTTTGACCTAGACTCGTTATCGAAGAAA
	GGATTTTCATCTTTCGTAAAGTTTAGCAAGTCCAAGTGGAATGTATACACATTCGGCGAG
	AGAATTATCAAGCCCAAGAACAAACAGGGCTATAGAGAAGACAAGAGAATCAACTTGACT
	TTTGAGATGAAAAAATTACTCAACGAATACAAGGTTTCATTTGATTTGGAGAACAACTTG
	ATTCCCAATTTGACATCAGCTAACTTGAAGGATACGTTCTGGAAGGAGTTATTCTTTATA
	TTCAAAACGACATTACAACTGCGTAATAGTGTTACAAACGGTAAAGAAGATGTATTAATC
	TCACCTGTAAAGAATGCCAAAGGAGAATTTTTCGTATCCGGTACTCACAATAAGACACTA
	CCACAGGATTGCGACGCTAACGGTGCGTATCATATTGCGTTGAAAGGATTAATGATACTT
	GAAAGAAATAACCTTGTTCGCGAAGAAAAAGACACCAAGAAGATCATGGCTATTAGCAAT
	GTTGATTGGTTTGAATACGTGCAAAAGAGGAGAGGTGTTTTGTAA

SEQ	ATGAACAATTATGACGAGTTCACAAAGCTATACCCTATCCAAAAAACTATCAGGTTCGAA
ID	TTGAAACCACAAGGGAGAACAATGGAACATCTGGAGACATTCAACTTTTTTGAAGAGGAC
NO:	AGAGACAGAGCGGAGAAATACAAAATTTTAAAAGAGGCCATCGATGAATATCACAAAAAG
129	TTTATCGACGAGCATTTAACAAACATGTCTTTGGACTGGAATTCACTTAAACAAATTTCT
	GAGAAATATTATAAGTCTCGGGAGGAAAAAGACAAAAAGGTCTTTTTGTCCGAGCAAAAG
	AGAATGAGACAAGAAATTGTCTCGGAGTTTAAAAAAGATGATCGGTTCAAAGATTTGTTT
	AGCAAGAAATTGTTTTCTGAATTGTTGAAGGAGGAGATATACAAGAAAGGCAACCATCAA
	GAAATAGATGCTTTGAAATCGTTTGACAAGTTCAGCGGTTACTTCATTGGTTTACATGAA
	AATAGGAAGAACATGTATAGCGACGGCGATGAGATCACCGCTATATCGAATAGAATCGTT
	AACGAAAATTTTCCGAAATTTTTGGATAATTTGCAAAAATACCAGGAAGCTAGGAAAAAG
	TACCCTGAATGGATAATAAAGGCGGAATCAGCTTTGGTGGCTCACAACATAAAGATGGAT
	GAAGTCTTCTCGCTGGAATATTTTAACAAAGTATTAAATCAGGAAGGAATCCAAAGATAC
	AACTTAGCCTTGGGTGGATACGTAACCAAATCAGGTGAGAAAATGATGGGCTTAAATGAT
	GCACTTAATCTAGCTCACCAATCCGAAAAGTCCTCTAAAGGGAGGATACACATGACACCA
	TTGTTTAAGCAAATCCTTTCGGAGAAAGAATCTTTTTCATATATCCCCGATGTTTTCACT
	GAGGATAGTCAATTGTTGCCCAGCATTGGTGGATTTTTTGCACAAATAGAAAATGATAAA
	GATGGTAACATCTTCGATAGAGCCTTGGAATTGATAAGCTCCTATGCAGAATACGATACG
	GAACGAATATACATTAGACAAGCTGACATCAACAGAGTAAGCAATGTTATTTTTGGTGAG
	TGGGGAACTTTAGGTGGATTAATGCGGGAGTACAAAGCTGACTCAATCAATGATATTAAT
	TTGGAACGTACGTGCAAAAAAGTCGATAAGTGGCTTGATAGTAAGGAGTTTGCTCTGTCG
	GATGTACTAGAAGCAATTAAGAGAACAGGAAACAATGATGCATTTAATGAATATATTAGT
	AAAATGAGGACGGCTAGAGAAAAGATAGACGCCGCACGTAAGGAAATGAAGTTTATTTCC
	GAGAAAATATCTGGCGATGAAGAGTCGATTCACATCATCAAGACCCTACTCGATTCTGTT
	CAGCAATTTCTCCATTTTTTTAACCTCTTCAAAGCAAGACAAGACATTCCCTTAGATGGG
	GCTTTTTATGCCGAATTTGATGAAGTTCATTCAAAGTTGTTTGCTATTGTTCCTCTTTAC
	AATAAGGTCCGTAATTACCTTACTAAAAATAACTTGAACACCAAGAAAATAAAGTTAAAC
	TTCAAGAATCCGACTCTTGCCAACGGGTGGGATCAGAATAAAGTTTATGATTATGCTAGC
	TTAATATTTCTAAGAGATGGGAATTATTACTTAGGAATCATCAATCCAAAGCGTAAGAAA
	AACATTAAATTTGAACAAGGGTCAGGCAATGGCCCATTCTATAGAAAAATGGTGTATAAG
	CAAATACCAGGACCTAACAAGAACTTGCCTCGCGTATTTTTAACTTCAACAAAGGGTAAA
	AAAGAATATAAACCAAGCAAAGAAATTATTGAAGGTTACGAAGCAGATAAACACATCAGA
	GGTGATAAGTTCGATCTGGATTTCTGCCATAAATTGATTGACTTTTTTAAGGAATCTATA
	GAAAAACATAAGGACTGGTCCAAATTTAATTTCTACTTCTCACCTACAGAAAGTTATGGT
	GACATTTCAGAATTTTATTTAGACGTTGAGAAACAAGGATATAGGATGCATTTTGAAAAT
	ATTTCAGCGGAAACCATCGACGAATACGTTGAGAAGGGTGATTTATTCTTGTTCCAAATT
	TACAATAAAGACTTCGTTAAAGCTGCAACCGGAAAGAAGGATATGCATACCATATATTGG
	AACGCTGCATTCTCGCCAGAAAACTTACAAGATGTCGTTGTAAAGCTTAATGGAGAAGCT
	GAGCTGTTCTATAGAGACAAGAGTGATATAAAAGAGATTGTGCATCGGGAAGGTGAAATT
	CTGGTGAACAGAACTTACAATGGTCGTACACCCGTTCCAGACAAAATACATAAAAAACTG
	ACCGATTATCATAATGGTAGGACAAAGGACTTGGGCGAGGCCAAGGAGTACCTCGATAAA
	GTTAGATATTTCAAGGCACACTATGATATTACGAAAGACAGGAGATATTTAAACGATAAA
	ATTTACTTTCATGTCCCTTTGACCCTTAACTTTAAAGCTAATGGTAAAAAGAATTTGAAC
	AAAATGGTAATTGAGAAGTTTTTATCGGACGAAAAAGCTCACATAATCGGAATCGACCGC
	GGAGAGAGAAATTTACTGTATTATAGTATCATCGACAGAAGTGGAAAGATTATTGATCAG
	CAATCTTTGAACGTCATTGATGGGTTTGACTATCGGGAAAAGTTAAATCAAAGGGAAATT
	GAAATGAAGGATGCGAGACAATCATGGAATGCCATTGGTAAAATTAAAGATCTCAAGGAG
	GGGTACTTATCAAAAGCTGTACACGAGATAACTAAAATGGCTATCCAATATAATGCAATT
	GTTGTAATGGAAGAATTGAATTATGGTTTTAAACGCGGCAGGTTTAAAGTCGAAAAACAA
	ATATACCAAAAGTTTGAAAACATGTTAATTGATAAGATGAACTATCTTGTTTTCAAAGAT
	GCACCTGATGAGAGTCCTGGCGGTGTGCTGAACGCCTATCAATTAACAAACCCATTAGAG
	TCCTTTGCTAAACTGGGTAAACAAACTGGCATTCTATTTTATGTTCCAGCCGCTTACACC
	TCAAAGATCGATCCAACGACCGGTTTTGTAAACTTATTTAATACTTCTTCCAAAACAAAC
	GCGCAAGAACGCAAAGAATTCCTACAAAAATTTGAATCAATATCCTATAGCGCAAAAGAT
	GGAGGTATATTCGCTTTCGCTTTTGACTACAGAAAGTTTGGCACTTCCAAGACAGATCAT
	AAAAATGTGTGGACCGCTTATACCAACGGAGAAAGGATGCGTTATATTAAAGAAAAAAAG
	AGGAACGAACTATTTGATCCATCGAAAGAAATTAAAGAAGCTTTGACAAGCAGCGGAATC
	AAATATGATGGAGGTCAAAACATACTTCCAGATATTCTCAGATCTAATAATAACGGTCTT
	ATTTACACGATGTATTCATCTTTTATCGCTGCCATCCAAATGCGTGTGTATGATGGCAAG
	GAAGATTATATTATATCTCCTATTAAAAATTCAAAGGGTGAATTTTTTCGCACGGATCCA
	AAAAGAAGAGAGCTTCCAATTGACGCCGATGCTAACGGTGCTTACAATATTGCATTGCGT
	GGTGAACTTACTATGAGAGCCATCGCCGAAAAGTTTGATCCGGACAGTGAAAAAATGGCG
	AAATTGGAGCTAAAGCACAAGGATTGGTTTGAATTCATGCAGACCCGTGGCGATTGA

SEQ	ATGACTAAAACGTTCGACTCCGAGTTTTTTAATCTCTATTCCTTGCAAAAGACCGTTAGG
ID	TTTGAATTGAAACCAGTTGGTGAAACTGCCTCATTTGTCGAAGACTTTAAAAACGAGGGA
NO:	TTGAAAAGAGTGGTTAGTGAAGATGAAAGAAGGGCAGTAGACTATCAAAAGGTTAAAGAA
130	ATCATTGACGATTACCACAGAGATTTTATAGAAGAATCTCTGAACTATTTTCCAGAGCAG
	GTTTCAAAAGATGCTCTAGAGCAAGCGTTTCATTTGTATCAAAAGTTGAAAGCAGCGAAG
	GTGGAAGAAAGGGAAAAAGCTTTAAAAGAATGGGAAGCATTACAGAAAAAATTGCGAGAA
	AAAGTCGTCAAATGTTTCAGCGACTCTAATAAAGCTCGCTTTTCTAGAATCGATAAAAAA
	GAATTGATTAAGGAAGATTTAATAAATTGGCTGGTAGCACAAAACAGAGAGGATGATATT
	CCTACTGTTGAAACGTTCAATAATTTTACTACTTACTTCACTGGTTTCCATGAGAACAGG
	AAGAATATTTACTCTAAAGATGATCACGCTACTGCTATAAGTTTTAGGTTGATTCACGAA
	AACTTGCCTAAATTTTTTGACAATGTCATCAGTTTTAACAAGTTGAAAGAAGGTTTCCCG
	GAATTAAAATTCGACAAAGTTAAAGAAGATTTAGAAGTAGATTACGACTTGAAGCATGCG
	TTTGAAATTGAATATTTCGTTAATTTCGTCACACAAGCTGGTATCGACCAATATAATTAC
	CTGCTTGGAGGCAAAACTCTAGAAGACGGTACGAAGAAACAAGGAATGAATGAACAGATT
	AATTTATTTAAGCAACAACAAACTCGCGATAAAGCTAGACAGATTCCAAAACTGATTCCA
	CTTTTCAAACAGATTCTATCTGAGAGAACTGAATCTCAGAGTTTTATCCCTAAGCAGTTC
	GAGTCTGATCAGGAACTATTCGATTCCCTGCAGAAATTGCATAACAACTGTCAAGATAAG
	TTTACCGTTTTGCAACAGGCGATCTTGGGATTGGCTGAGGCAGATCTTAAAAAGGTCTTT
	ATTAAAACTAGTGATCTAAACGCATTGTCTAACACTATTTTTGGAAATTATTCTGTGTTC
	TCAGACGCGCTCAATTTATATAAAGAGTCGCTAAAAACTAAAAAGGCTCAAGAAGCTTTT
	GAAAAGTTGCCTGCACATAGTATTCATGATTTAATCCAATACTTAGAACAATTTAATTCG
	TCTCTCGATGCTGAAAAGCAACAGTCTACCGATACTGTATTAAACTACTTTATTAAAACC
	GACGAATTATATAGTCGTTTCATTAAATCCACCTCTGAGGCATTCACCCAAGTACAACCT
	CTCTTTGAACTGGAAGCTTTGAGCTCCAAAAGAAGACCCCCAGAAAGTGAAGATGAGGGG
	GCTAAAGGCCAAGAAGGTTTCGAACAAATTAAGAGAATCAAAGCTTATCTAGACACTCTA
	ATGGAGGCTGTCCACTTTGCTAAGCCTTTGTATCTTGTCAAGGGTAGAAAGATGATAGAG
	GGTCTAGACAAGGATCAAAGCTTCTACGAAGCGTTTGAAATGGCCTACCAGGAGTTGGAG
	TCTTTAATCATCCCCATTTACAATAAGGCCAGATCTTACCTGTCTAGGAAGCCATTTAAA
	GCGGATAAATTCAAAATTAATTTTGACAATAATACACTTCTATCTGGGTGGGATGCTAAC
	AAGGAGACGGCTAACGCCAGCATATTGTTTAAGAAGGATGGTTTATACTACCTGGGAATC
	ATGCCAAAAGGCAAAACTTTCTTGTTCGATTATTTCGTTAGTTCAGAAGATTCTGAAAAG
	TTGAAACAACGGAGACAGAAAACCGCAGAGGAAGCGCTCGCACAGGATGGAGAATCCTAT
	TTTGAAAAAATACGGTATAAACTCCTACCAGGTGCTAGTAAGATGTTGCCAAAGGTATTT
	TTTAGCAATAAAAATATTGGGTTTTACAATCCCTCAGATGATATTCTACGAATTCGGAAT
	ACGGCCTCTCATACTAAGAATGGTACTCCCCAGAAGGGTCATTCCAAGGTAGAATTTAAC
	TTGAATGACTGTCACAAAATGATTGATTTTTTTAAATCTTCCATACAGAAACATCCCGAG
	TGGGGATCCTTTGGTTTCACTTTTTCTGATACGTCGGACTTTGAAGATATGAGTGCTTTC
	TACCGAGAAGTTGAAAATCAAGGTTACGTTATAAGTTTTGATAAAATAAAAGAAACTTAC
	ATTCAGTCTCAAGTTGAGCAAGGTAACTTATATTTATTTCAAATTTACAACAAAGATTTT
	AGTCCGTATTCAAAGGGAAAGCCAAACCTGCACACTTTATACTGGAAAGCTCTGTTTGAA
	GAGGCTAATTTGAATAACGTAGTGGCTAAGCTAAACGGCGAAGCAGAAATCTTTTTCAGA
	AGACACAGTATCAAAGCATCTGATAAAGTGGTACATCCTGCTAATCAAGCTATAGATAAT
	AAGAATCCCCATACTGAGAAGACGCAGTCCACATTTGAATATGACTTGGTCAAAGACAAA
	AGATATACCCAAGACAAATTTTTTTTTCATGTACCGATATCTTTAAACTTTAAGGCTCAG
	GGCGTTTCAAAGTTTAATGATAAGGTAAATGGATTCTTAAAGGGCAATCCCGACGTTAAT
	ATAATCGGTATAGATCGAGGTGAGAGACATCTTTTATACTTTACCGTGGTGAATCAAAAA
	GGAGAAATATTAGTGCAAGAGTCCTTGAATACATTAATGTCTGACAAGGGTCATGTCAAC
	GATTATCAACAGAAATTGGACAAGAAGGAACAGGAAAGGGACGCTGCCAGGAAGTCCTGG
	ACGACAGTAGAAAATATTAAAGAATTAAAAGAAGGTTATTTATCACATGTGGTTCATAAA
	CTTGCACATTTAATCATCAAATATAACGCAATAGTGTGCTTGGAAGATCTTAATTTTGGC
	TTCAAGAGGGGTAGGTTCAAGGTCGAAAAACAGGTCTACCAGAAGTTCGAGAAAGCTCTG
	ATCGATAAATTGAATTATCTTGTTTTCAAAGAAAAAGAATTAGGAGAAGTTGGTCATTAT
	CTTACAGCATACCAACTCACTGCACCATTTGAAAGCTTCAAAAAGCTAGGCAAGCAATCT
	GGGATTTTGTTCTATGTTCCGGCTGATTATACATCAAAGATAGATCCTACCACAGGCTTT
	GTAAATTTTTTAGATCTTAGGTACCAATCCGTTGAAAAAGCTAAACAGTTGCTGTCCGAT
	TTTAATGCGATAAGATTTAATAGTGTTCAGAATTATTTTGAGTTCGAAATTGATTATAAA
	AAATTGACACCAAAACGTAAAGTAGGAACACAATCTAAATGGGTTATTTGTACCTATGGA
	GATGTTAGATACCAAAACAGAAGAAATCAGAAAGGTCACTGGGAAACTGAAGAAGTTAAC
	GTTACTGAAAAACTTAAAGCTCTATTTGCGAGCGATTCAAAAACGACGACGGTGATCGAT
	TATGCAAATGATGATAACCTTATTGATGTAATTCTGGAACAAGATAAGGCATCATTTTTT
	AAAGAACTACTATGGTTGTTAAAGCTAACCATGACCCTAAGGCACTCCAAGATAAAGTCA
	GAGGATGATTTTATCCTCTCTCCAGTGAAAAACGAACAAGGTGAGTTTTACGACTCAAGA
	AAGGCGGGTGAAGTCTGGCCTAAGGATGCTGATGCCAATGGAGCTTATCACATCGCTCTG
	AAGGGGCTATGGAACTTACAGCAAATTAACCAATGGGAAAAAGGTAAAACTTTAAACCTC
	GCCATAAAGAACCAGGATTGGTTCAGCTTTATCCAAGAAAAACCATATCAAGAATAA

SEQ	ATGCACACAGGAGGTCTACTCTCGATGGATGCTAAGGAATTTACCGGTCAATATCCGCTG
ID	TCCAAAACTTTGCGTTTTGAGCTTAGACCTATTGGCCGAACGTGGGATAACCTAGAGGCT
NO:	TCTGGTTATTTGGCGGAAGATAGACATAGAGCTGAGTGTTATCCCCGAGCTAAAGAATTG
131	CTGGATGATAACCACAGGGCGTTCCTGAATAGAGTTCTACCGCAAATCGATATGGATTGG
	CATCCAATTGCTGAAGCTTTCTGCAAGGTGCACAAAAATCCAGGTAATAAAGAATTGGCT
	CAGGATTATAATTTGCAGCTTAGTAAGAGAAGAAAAGAAATTTCCGCTTATTTGCAGGAT
	GCTGATGGATACAAGGGGTTGTTCGCGAAACCTGCCCTGGACGAAGCTATGAAAATAGCT
	AAGGAAAACGGCAATGAATCTGATATTGAAGTTTTGGAAGCCTTCAATGGATTTTCCGTT
	TATTTCACTGGTTATCATGAGAGTAGGGAGAATATATACTCAGACGAAGATATGGTATCC
	GTCGCCTATCGCATAACTGAAGATAATTTTCCAAGGTTCGTGTCGAACGCGTTAATTTTT
	GATAAACTAAATGAATCGCACCCGGATATTATTTCGGAAGTGTCCGGTAATCTGGGGGTA
	GACGATATTGGTAAATATTTTGATGTGTCCAACTACAATAATTTCCTTAGTCAAGCAGGA
	ATTGATGACTACAACCATATTATAGGAGGGCATACAACTGAAGACGGTCTCATTCAAGCT
	TTTAACGTAGTGTTAAACCTAAGGCACCAAAAAGACCCAGGTTTTGAGAAAATTCAATTT
	AAGCAACTCTACAAGCAGATACTGAGCGTTAGGACTAGTAAGTCATATATCCCAAAGCAA
	TTCGATAACTCAAAGGAAATGGTCGACTGTATATGCGACTACGTCTCAAAAATAGAAAAA
	TCTGAAACAGTAGAAAGAGCTCTGAAATTGGTAAGAAATATATCTTCTTTTGATTTAAGA
	GGTATTTTCGTAAATAAAAAAAACCTTCGAATTTTGTCTAATAAGTTAATTGGAGACTGG
	GACGCAATAGAGACAGCTTTGATGCACAGTTCCAGCAGTGAAAACGATAAGAAATCAGTG
	TATGACTCTGCAGAGGCATTCACCCTTGATGATATCTTCAGTTCTGTGAAAAAGTTCAGC
	GACGCCTCCGCTGAGGATATAGGAAACCGCGCTGAAGACATATGTCGTGTTATCTCAGAA
	ACAGCTCCTTTCATTAACGACTTAAGGGCTGTAGATTTGGATTCTTTAAATGATGACGGC
	TATGAAGCGGCCGTGTCTAAAATACGGGAATCTCTTGAACCCTACATGGATCTATTTCAC
	GAATTGGAGATCTTTAGCGTGGGTGATGAGTTTCCTAAATGTGCTGCCTTTTATAGCGAG
	TTGGAAGAGGTCTCAGAACAACTGATTGAAATCATTCCTTTATTTAACAAAGCAAGAAGT
	TTTTGCACAAGGAAAAGGTATTCAACCGACAAAATCAAAGTCAATTTAAAATTCCCTACT
	CTGGCAGATGGATGGGATCTAAATAAAGAAAGGGATAACAAAGCCGCAATTCTAAGAAAA
	GACGGTAAATACTACCTGGCAATTTTAGACATGAAGAAAGATCTCAGTAGTATTCGTACG
	AGCGATGAGGACGAGTCTTCTTTTGAAAAGATGGAATATAAATTGCTCCCTTCTCCTGTG
	AAAATGCTTCCAAAAATTTTTGTTAAATCGAAAGCCGCCAAAGAAAAGTACGGGTTGACC
	GATAGAATGTTAGAATGCTACGATAAAGGTATGCATAAGTCGGGTAGTGCTTTTGATTTG
	GGTTTTTGTCATGAATTGATCGATTACTATAAGCGCTGCATTGCCGAGTACCCAGGCTGG
	GATGTTTTCGACTTTAAATTTCGTGAGACAAGCGATTACGGATCCATGAAAGAATTTAAT
	GAAGACGTCGCTGGCGCAGGTTACTATATGTCACTTAGAAAGATTCCATGTTCCGAAGTT
	TATCGTTTACTGGACGAGAAGTCAATTTACTTGTTTCAAATATATAATAAGGATTATAGC
	GAAAACGCACATGGGAATAAGAATATGCATACGATGTATTGGGAGGGCTTGTTCTCACCA
	CAAAATTTGGAATCACCAGTCTTCAAATTGTCCGGAGGCGCAGAACTTTTTTTCAGAAAG
	TCATCTATTCCTAATGACGCTAAAACGGTACATCCGAAAGGTTCAGTTCTTGTTCCCAGA
	AACGACGTCAATGGTAGAAGAATACCAGACTCGATCTACAGAGAGTTGACAAGGTATTTT
	AACCGTGGGGATTGCAGGATCAGTGATGAAGCTAAGTCTTACCTGGACAAGGTCAAGACA
	AAAAAAGCGGACCATGACATTGTTAAGGATAGAAGATTTACTGTAGATAAGATGATGTTC
	CATGTTCCGATTGCCATGAATTTTAAAGCTATAAGTAAACCAAATCTTAATAAGAAAGTT
	ATTGATGGCATAATAGATGATCAAGATTTGAAAATCATCGGTATCGATCGTGGTGAGAGA
	AATCTTATTTATGTGACCATGGTCGATAGGAAGGGGAATATATTGTATCAAGACAGTCTT
	AATATTTTAAATGGATACGATTACCGCAAAGCTTTAGACGTGAGGGAATATGATAACAAA
	GAAGCTAGAAGGAATTGGACTAAAGTAGAAGGTATTAGAAAAATGAAAGAAGGTTATTTA
	TCTTTAGCTGTTAGTAAATTGGCCGATATGATCATCGAAAATAATGCTATAATCGTAATG
	GAAGATTTGAATCACGGGTTTAAGGCAGGTCGTTCCAAAATTGAAAAGCAGGTGTATCAA
	AAATTCGAATCAATGTTAATCAACAAGTTAGGATACATGGTGCTAAAAGACAAGTCCATT
	GACCAGTCTGGTGGAGCCCTTCATGGTTACCAATTAGCCAATCATGTTACGACCTTAGCT
	AGCGTGGGTAAACAATGTGGAGTAATTTTTTACATACCTGCAGCTTTTACTTCGAAGATT
	GATCCCACCACGGGCTTTGCTGATTTATTCGCTCTCTCTAATGTGAAGAATGTCGCTTCT
	ATGAGAGAGTTCTTCTCCAAAATGAAGTCAGTAATATATGACAAGGCGGAAGGCAAATTC
	GCCTTTACATTTGATTATTTGGATTATAACGTTAAAAGCGAATGTGGACGTACCTTATGG
	ACTGTGTATACAGTTGGTGAACGCTTCACCTACTCTAGAGTAAACCGAGAGTATGTTCGG
	AAAGTCCCAACAGATATCATCTATGATGCATTACAAAAAGCTGGTATTAGCGTCGAAGGT
	GACCTTAGAGATAGAATCGCGGAAAGCGACGGTGACACATTAAAGTCTATATTCTACGCT
	TTTAAATACGCGTTGGATATGAGAGTCGAAAACAGAGAGGAAGACTATATACAGTCACCT
	GTGAAGAATGCTTCTGGTGAGTTCTTTTGTTCAAAAAACGCCGGAAAGTCTTTGCCGCAG
	GATTCAGATGCAAATGGTGCCTATAATATAGCTCTGAAAGGGATCCTACAACTCAGAATG
	TTGAGCGAACAATACGATCCAAATGCAGAATCGATTAGATTGCCACTTATAACTAACAAG
	GCATGGTTAACTTTTATGCAATCCGGTATGAAAACTTGGAAGAATTAA

SEQ	ATGGATTCTCTTAAGGATTTCACTAATTTATATCCAGTCTCGAAAACATTGCGGTTCGAA
ID	TTGAAACCAGTTGGGAAAACTCTAGAAAACATTGAAAAAGCCGGTATATTGAAAGAAGAT
NO:	GAACACAGAGCGGAATCCTACCGCCGGGTAAAAAAGATAATTGACACATACCATAAAGTG
132	TTTATTGACAGCTCCTTAGAGAACATGGCTAAAATGGGGATAGAAAATGAAATCAAGGCT
	ATGCTGCAGTCTTTTTGTGAACTCTATAAGAAAGACCACAGGACAGAAGGAGAAGATAAA
	GCTCTTGATAAAATTAGAGCTGTTCTTAGAGGTTTAATCGTTGGGGCTTTCACTGGTGTA
	TGTGGAAGACGAGAAAACACAGTACAAAATGAAAAGTACGAGAGTTTGTTCAAAGAAAAA
	TTGATAAAGGAAATTTTGCCAGATTTCGTGTTGTCCACCGAGGCTGAGTCTCTTCCATTC
	AGCGTTGAAGAAGCAACAAGGAGCTTAAAAGAGTTTGACTCATTCACTTCTTATTTTGCT
	GGTTTTTACGAAAATAGAAAGAATATTTATTCCACGAAACCGCAAAGTACTGCGATAGCC
	TACAGATTAATTCATGAAAACTTGCCTAAATTTATAGATAATATTTTGGTCTTCCAGAAG
	ATTAAAGAACCAATCGCTAAAGAACTTGAGCACATAAGAGCAGATTTTAGCGCAGGCGGA
	TATATCAAAAAAGATGAACGGCTAGAAGACATATTCTCATTAAATTACTACATTCATGTC
	CTTTCTCAAGCTGGTATAGAAAAATATAATGCTTTAATCGGGAAGATAGTGACGGAAGGT
	GATGGTGAAATGAAAGGTCTTAATGAACATATTAACTTATATAACCAACAGAGGGGTCGA
	GAGGATAGGTTGCCCTTGTTTAGGCCTCTATACAAGCAAATCCTGTCCGATAGAGAGCAA
	TTGTCTTATTTACCTGAATCATTTGAAAAAGATGAAGAGCTGCTTAGAGCACTTAAGGAA
	TTTTACGATCACATCGCCGAAGACATCTTGGGTAGAACACAGCAATTGATGACTTCAATT
	TCTGAATACGACTTGTCCCGTATTTATGTCAGAAATGATTCTCAACTTACAGACATCTCG
	AAGAAAATGCTAGGAGATTGGAACGCCATTTATATGGCTAGAGAACGAGCCTACGACCAC
	GAACAGGCTCCTAAACGTATTACTGCTAAATACGAACGTGATAGAATCAAGGCCTTAAAA
	GGTGAAGAGTCAATTTCATTGGCGAATCTGAACAGCTGTATAGCTTTCTTGGACAATGTA
	AGGGATTGTCGAGTTGACACATACCTATCAACTTTGGGGCAGAAAGAGGGTCCTCATGGC
	TTAAGTAACTTGGTGGAAAACGTCTTCGCCTCATATCATGAAGCAGAACAGTTATTGTCG
	TTTCCTTACCCCGAAGAGAACAACCTTATTCAGGACAAAGACAATGTAGTTTTGATCAAA
	AACCTATTGGATAATATAAGTGATTTACAACGTTTCCTTAAACCTTTGTGGGGAATGGGC
	GATGAACCTGACAAAGACGAAAGGTTTTACGGTGAATACAACTATATTAGAGGAGCGCTT
	GACCAGGTAATACCTTTGTACAATAAAGTAAGGAACTACTTGACTCGTAAACCATATTCT
	ACTAGAAAAGTTAAATTGAACTTTGGTAATTCACAGCTGCTGAGTGGTTGGGATCGTAAT
	AAAGAAAAAGATAACTCCTGTGTTATCTTGCGAAAAGGACAAAACTTTTACTTGGCAATT
	ATGAACAACCGTCACAAAAGGTCCTTCGAGAACAAAGTTCTGCCTGAATACAAAGAAGGT
	GAACCATATTTTGAAAAAATGGACTATAAATTCCTGCCAGATCCTAATAAAATGTTGCCT
	AAGGTCTTCTTGTCTAAAAAAGGTATAGAAATATATAAACCATCCCCGAAGTTGCTGGAG
	CAATATGGTCATGGAACGCACAAAAAAGGTGACACTTTTAGTATGGATGACTTGCACGAG
	TTGATTGATTTTTTTAAACATTCCATTGAAGCGCACGAAGATTGGAAACAATTTGGTTTC
	AAGTTCTCTGACACAGCCACTTACGAAAATGTATCGTCCTTTTATAGAGAAGTGGAAGAT
	CAGGGTTATAAACTGTCATTCCGTAAGGTTAGTGAAAGCTATGTGTACTCGTTGATCGAT
	CAAGGGAAGCTTTATCTTTTTCAAATCTATAATAAAGATTTCTCTCCTTGTTCAAAGGGC
	ACACCTAATCTTCATACACTATACTGGAGAATGCTTTTCGATGAAAGAAATTTGGCTGAT
	GTGATCTATAAATTAGACGGTAAAGCTGAGATTTTTTTCAGAGAGAAATCCCTGAAAAAC
	GACCATCCAACTCATCCGGCAGGTAAACCGATTAAAAAGAAATCCCGGCAAAAAAAGGGC
	GAAGAGAGTTTATTCGAGTATGATTTAGTTAAGGACAGACATTATACAATGGACAAATTT
	CAATTTCATGTGCCCATTACTATGAACTTTAAGTGTAGTGCAGGGTCTAAGGTTAATGAT
	ATGGTAAACGCACATATTAGAGAAGCTAAAGATATGCACGTCATCGGTATTGATCGCGGA
	GAAAGAAATTTACTTTACATTTGCGTTATCGATTCTAGGGGCACCATCTTGGATCAAATC
	TCTTTGAACACTATAAATGATATTGACTATCATGATCTACTAGAGAGTCGGGATAAAGAC
	AGGCAACAAGAAAGAAGAAATTGGCAAACAATTGAAGGTATTAAAGAATTAAAGCAAGGC
	TATCTAAGCCAGGCTGTACACAGAATTGCCGAATTAATGGTAGCATATAAAGCTGTCGTA
	GCTCTAGAAGACTTGAACATGGGTTTCAAAAGAGGGCGCCAGAAGGTCGAAAGTAGTGTT
	TATCAACAATTTGAAAAACAGTTAATAGATAAGTTGAATTATCTAGTGGATAAAAAAAAG
	CGTCCTGAGGACATTGGCGGTTTATTAAGAGCCTACCAATTCACTGCGCCATTTAAATCG
	TTCAAAGAAATGGGTAAACAAAACGGTTTTCTATTCTACATCCCCGCATGGAATACCTCA
	AATATAGATCCAACTACCGGTTTCGTCAACTTATTTCATGCTCAATATGAGAATGTGGAC
	AAAGCAAAATCATTCTTTCAAAAATTTGATAGCATTAGCTACAATCCTAAAAAAGATTGG
	TTTGAATTTGCGTTCGATTATAAAAATTTCACCAAGAAGGCTGAAGGTTCCAGATCTATG
	TGGATATTGTGCACCCACGGAAGTAGAATTAAGAACTTCCGTAATTCACAGAAAAACGGC
	CAGTGGGACAGCGAAGAATTCGCCCTAACCGAAGCTTTCAAAAGTCTTTTCGTAAGATAC
	GAGATAGACTATACAGCTGATCTAAAGACAGCTATTGTGGATGAGAAGCAAAAAGACTTC
	TTTGTCGACCTTCTTAAGTTGTTCAAGTTAACTGTGCAGATGAGAAATAGTTGGAAGGAA
	AAAGACCTAGATTACTTGATTAGCCCAGTCGCTGGTGCAGATGGCAGATTTTTTGATACA
	CGTGAAGGCAATAAATCACTACCAAAAGACGCGGACGCTAATGGCGCATACAACATCGCA
	TTGAAGGGTTTGTGGGCTCTCAGGCAGATTAGGCAGACAAGTGAGGGTGGTAAGCTTAAG
	CTGGCGATTTCTAATAAGGAATGGTTACAGTTTGTTCAAGAAAGATCCTACGAAAAAGAT
	TAA

SEQ	ATGAACAATGGTACTAATAATTTTCAAAACTTCATAGGGATTTCTAGCCTTCAAAAGACA
ID	TTGAGAAATGCTTTAATTCCAACAGAAACGACTCAACAATTCATAGTGAAAAATGGTATT
NO:	ATAAAAGAAGACGAGTTGCGTGGCGAGAATAGACAAATTTTGAAAGATATCATGGATGAC
133	TACTACAGAGGGTTCATCTCCGAAACATTGTCTTCTATTGACGACATTGACTGGACCAGC
	TTATTCGAAAAAATGGAAATACAGCTGAAGAACGGAGATAACAAGGACACTCTTATAAAG
	GAGCAAACGGAATATAGAAAGGCTATACACAAAAAGTTTGCTAATGACGATAGATTTAAA
	AACATGTTTAGTGCGAAGTTAATTTCTGATATTCTACCCGAGTTTGTCATTCATAATAAT
	AACTACTCTGCATCTGAAAAAGAGGAGAAGACCCAGGTTATAAAGTTGTTTTCAAGATTT
	GCCACATCATTTAAAGACTACTTCAAGAACAGGGCGAATTGCTTCTCTGCTGATGATATT
	AGCTCTTCCAGCTGTCATAGAATTGTTAACGATAATGCCGAAATTTTTTTTAGTAATGCC
	TTGGTATATAGACGCATAGTCAAGTCACTAAGCAATGATGATATAAACAAGATTAGTGGT
	GATATGAAAGATAGCCTTAAAGAAATGAGCCTTGAAGAGATATATTCATATGAGAAGTAC
	GGTGAATTTATAACTCAAGAAGGAATTTCTTTTTATAACGATATTTGTGGTAAGGTTAAT
	TCTTTTATGAATTTGTATTGCCAGAAGAACAAGGAAAATAAGAATCTATATAAACTACAA
	AAGTTGCATAAACAGATTTTGTGTATAGCTGATACATCCTACGAAGTTCCGTATAAATTT
	GAATCTGATGAGGAAGTTTATCAATCGGTAAACGGTTTTCTTGACAACATTTCCAGCAAA
	CATATCGTTGAGAGACTACGTAAAATTGGAGACAACTATAATGGTTACAATCTAGATAAA
	ATATACATAGTGTCCAAGTTTTATGAGTCTGTCTCTCAAAAGACATATCGTGATTGGGAG
	ACCATTAATACTGCACTTGAAATTCATTATAACAACATATTGCCTGGTAACGGGAAGAGT
	AAAGCTGATAAGGTTAAAAAGGCCGTCAAAAACGACTTGCAAAAGTCTATTACCGAGATA
	AATGAATTAGTGTCAAACTACAAACTATGCTCAGATGATAATATTAAAGCGGAAACATAC
	ATCCACGAAATTTCCCACATACTGAATAACTTTGAAGCTCAGGAGCTTAAATATAACCCG
	GAAATACACTTGGTTGAGAGCGAGTTAAAAGCATCTGAGTTGAAAAATGTATTAGACGTC
	ATCATGAATGCGTTTCATTGGTGTTCAGTTTTCATGACTGAAGAATTAGTCGACAAAGAT
	AACAATTTTTATGCCGAATTAGAGGAAATATATGATGAAATTTATCCCGTAATTAGTTTA
	TACAATCTAGTTAGAAATTATGTTACACAAAAGCCGTATAGTACCAAGAAAATAAAGCTT
	AATTTCGGAATACCTACGCTTGCTGATGGTTGGTCAAAAAGTAAAGAATATAGCAATAAT
	GCAATAATTTTAATGAGAGATAACCTATATTATTTGGGTATTTTTAACGCTAAGAACAAA
	CCAGACAAGAAAATAATTGAAGGTAATACATCTGAAAACAAGGGCGACTATAAAAAGATG
	ATATACAATTTGCTCCCAGGTCCTAATAAAATGATTCCTAAGGTTTTCCTGAGTAGCAAG
	ACTGGCGTTGAAACTTACAAGCCTAGTGCGTATATCCTGGAGGGTTATAAACAGAACAAG
	CATATCAAATCCTCTAAGGACTTCGATATCACCTTTTGCCATGACTTAATCGATTATTTT
	AAAAATTGTATCGCAATTCATCCAGAATGGAAAAATTTCGGATTTGATTTTAGTGATACC
	AGCACTTACGAGGATATCTCTGGGTTCTACAGAGAAGTGGAGTTGCAGGGCTACAAAATC
	GATTGGACTTACATATCTGAAAAGGACATAGATTTGCTGCAGGAGAAAGGTCAGCTATAT
	TTGTTTCAAATCTACAACAAAGACTTTTCTAAAAAGTCTACCGGTAATGACAATCTGCAC
	ACAATGTACTTGAAGAACTTATTCTCCGAGGAGAACTTAAAGGACATTGTACTCAAGTTG
	AATGGAGAAGCCGAGATTTTTTTTAGAAAGAGCAGTATAAAGAATCCTATAATCCACAAG
	AAGGGCTCAATTCTCGTGAATAGGACGTATGAGGCAGAAGAAAAGGACCAATTTGGGAAT
	ATACAAATTGTAAGAAAAAACATCCCAGAAAATATCTACCAGGAATTATATAAGTATTTT
	AATGACAAATCTGATAAGGAACTGTCTGACGAAGCCGCTAAGCTCAAGAATGTTGTGGGC
	CACCATGAAGCTGCTACTAATATAGTGAAGGACTACAGATATACCTACGATAAATATTTC
	CTGCATATGCCAATTACTATAAACTTCAAAGCAAATAAAACAGGTTTTATAAATGATAGA
	ATCCTGCAGTATATTGCTAAAGAAAAGGATTTACATGTAATTGGGATTGATAGAGGTGAA
	CGCAATCTGATCTATGTCAGCGTAATAGATACTTGTGGTAATATTGTGGAACAAAAGTCC
	TTTAATATTGTGAACGGATATGATTACCAAATCAAGTTGAAACAACAAGAGGGAGCACGC
	CAAATTGCCCGTAAGGAATGGAAAGAGATAGGTAAGATCAAGGAAATTAAGGAAGGTTAT
	CTTTCATTAGTTATTCACGAAATTTCGAAGATGGTAATCAAATACAACGCAATAATTGCT
	ATGGAGGACCTGTCATATGGATTTAAGAAAGGTAGATTCAAGGTTGAGAGACAGGTATAC
	CAGAAATTTGAAACTATGTTGATCAACAAATTAAATTACTTAGTCTTTAAGGACATATCA
	ATAACGGAAAACGGCGGGCTTTTAAAAGGGTATCAACTTACATACATACCTGATAAGTTG
	AAAAATGTGGGTCATCAGTGTGGGTGCATCTTTTATGTTCCAGCCGCTTACACATCAAAA
	ATCGATCCTACTACTGGGTTCGTAAACATATTTAAATTTAAAGATCTAACCGTTGATGCA
	AAAAGAGAGTTTATCAAGAAATTTGATAGCATTAGGTACGATTCAGAAAAAAATCTATTC
	TGTTTTACTTTTGACTACAACAACTTTATAACGCAGAATACAGTGATGTCAAAATCGTCC
	TGGTCAGTGTATACTTATGGTGTTAGAATTAAGAGACGTTTCGTAAACGGTCGTTTTTCT
	AACGAGTCCGATACAATCGACATCACTAAAGATATGGAAAAAACTTTGGAAATGACAGAT
	ATAAACTGGAGAGATGGTCACGACCTTAGACAAGATATAATCGATTATGAAATCGTACAG
	CATATTTTTGAAATTTTTCGCTTAACAGTTCAGATGCGTAACTCTCTTAGTGAGCTAGAA
	GATAGAGATTATGATAGACTTATCTCGCCTGTTCTTAACGAAAATAATATCTTCTATGAC
	TCGGCAAAAGCCGGTGATGCACTTCCAAAAGATGCTGATGCAAATGGCGCGTACTGCATC
	GCATTGAAGGGGCTCTACGAGATTAAACAAATCACCGAAAACTGGAAAGAAGATGGTAAA
	TTTTCTAGGGATAAGTTGAAAATCAGTAATAAAGATTGGTTCGATTTTATACAAAATAAG
	CGATACTTATAG

SEQ	ATGACCAATAAGTTTACTAATCAATACTCATTGTCTAAAACGTTAAGATTCGAGTTAATT
ID	CCCCAGGGAAAGACACTAGAATTTATTCAAGAAAAAGGTCTTCTCTCTCAGGATAAACAA
NO:	AGAGCAGAATCATACCAGGAGATGAAAAAAACCATAGATAAATTTCATAAGTACTTCATC
134	GACTTGGCACTATCGAACGCCAAGCTAACACATTTGGAAACCTACCTGGAGTTGTATAAT
	AAATCGGCAGAGACGAAAAAGGAACAAAAATTCAAGGATGACCTGAAGAAGGTTCAAGAT
	AATCTGCGAAAGGAAATAGTGAAGTCGTTTAGTGATGGTGATGCAAAGTCAATCTTTGCT
	ATTTTAGACAAGAAGGAATTAATAACCGTGGAACTTGAAAAGTGGTTTGAAAATAACGAA
	CAGAAAGATATTTACTTCGACGAAAAATTTAAAACGTTTACTACGTACTTTACAGGGTTC
	CATCAGAACCGCAAAAACATGTACTCCGTTGAACCAAACTCTACTGCAATCGCCTACAGA
	TTAATACACGAAAATTTGCCTAAGTTTTTAGAAAATGCAAAGGCTTTTGAAAAGATAAAG
	CAAGTCGAATCGTTACAGGTAAACTTTCGCGAATTAATGGGCGAATTTGGAGATGAAGGT
	CTTATTTTTGTCAATGAATTAGAGGAAATGTTTCAAATTAATTATTATAACGATGTCTTG
	AGTCAGAACGGCATTACTATCTACAACTCAATTATCAGTGGTTTCACTAAGAATGATATA
	AAATATAAAGGTTTGAATGAATACATTAATAATTATAATCAAACTAAAGATAAGAAGGAC
	AGGCTTCCGAAATTGAAGCAATTGTACAAGCAGATTCTAAGTGATAGGATTAGTTTGTCT
	TTCTTGCCAGACGCATTTACTGATGGCAAGCAAGTCTTAAAGGCTATATTCGATTTCTAC
	AAGATTAACCTACTTTCGTACACAATTGAAGGTCAAGAAGAATCTCAAAATCTGCTGCTT
	TTGATTAGGCAAACTATAGAAAATTTGTCGTCCTTTGACACTCAAAAAATTTACCTGAAG
	AATGATACACACCTGACTACAATATCACAGCAGGTCTTTGGGGATTTTTCTGTCTTCTCC
	ACGGCCCTAAACTATTGGTATGAGACAAAAGTTAATCCAAAATTTGAAACAGAATATAGT
	AAGGCGAATGAAAAAAAGAGAGAAATTTTGGATAAAGCGAAGGCAGTATTCACAAAACAA
	GACTATTTTTCTATCGCATTTCTCCAAGAAGTCTTATCCGAATATATTTTGACACTCGAT
	CACACCTCTGATATAGTTAAGAAACATTCGTCCAACTGCATCGCAGATTACTTCAAGAAT
	CACTTCGTGGCTAAGAAAGAAAACGAAACGGATAAAACTTTTGACTTCATTGCTAACATA
	ACCGCTAAATACCAATGTATTCAGGGCATATTAGAAAATGCAGACCAGTACGAAGACGAG
	TTAAAACAGGACCAAAAGTTAATAGATAATCTAAAGTTTTTCTTAGATGCTATACTTGAG
	TTATTACATTTTATAAAGCCATTGCATCTAAAATCGGAAAGTATTACTGAAAAAGACACT
	GCGTTCTATGATGTGTTCGAAAATTATTATGAGGCTTTATCTTTATTGACCCCCCTTTAC
	AACATGGTCCGCAATTATGTTACTCAGAAGCCTTACTCTACTGAAAAGATCAAATTAAAC
	TTTGAAAATGCTCAGTTGCTGAATGGTTGGGATGCCAATAAGGAAGGTGACTACCTGACG
	ACTATTCTAAAAAAAGACGGTAATTATTTCTTAGCAATCATGGATAAAAAACATAACAAG
	GCATTTCAAAAATTTCCAGAAGGAAAAGAAAACTATGAAAAGATGGTTTATAAATTGTTG
	CCTGGAGTTAATAAAATGTTGCCAAAAGTTTTTTTTAGCAATAAGAACATAGCTTACTTT
	AATCCATCTAAGGAACTGCTCGAGAACTACAAGAAGGAAACACATAAAAAAGGTGATACA
	TTTAATTTGGAACATTGCCATACTCTGATTGATTTTTTTAAGGACTCTCTTAATAAACAT
	GAAGACTGGAAATATTTTGATTTTCAATTTTCGGAAACTAAATCATACCAAGATCTAAGT
	GGATTTTACAGAGAAGTTGAACACCAAGGTTATAAGATTAACTTCAAGAATATAGATTCT
	GAATACATTGATGGTCTTGTAAACGAGGGTAAACTATTCCTGTTCCAAATCTACTCTAAG
	GACTTCTCACCTTTTTCCAAAGGAAAACCTAATATGCATACGTTGTACTGGAAGGCTCTA
	TTTGAAGAACAAAATTTGCAAAATGTAATCTACAAACTGAACGGCCAAGCTGAAATATTC
	TTCAGAAAAGCCTCAATTAAGCCAAAAAACATTATTCTTCATAAAAAGAAGATCAAGATT
	GCGAAGAAACATTTTATTGATAAGAAGACCAAGACTTCCGAAATTGTACCAGTACAAACA
	ATCAAGAATCTCAATATGTATTATCAAGGCAAGATAAGTGAGAAAGAGTTAACCCAGGAT
	GATTTACGTTATATAGACAATTTCTCTATATTCAACGAGAAGAACAAAACAATAGACATT
	ATCAAAGATAAAAGGTTTACTGTTGACAAATTTCAATTTCATGTGCCTATCACAATGAAC
	TTTAAGGCCACAGGTGGTTCGTACATTAATCAAACTGTTTTAGAATATCTGCAAAATAAC
	CCAGAGGTCAAGATCATCGGTCTTGATAGGGGTGAGAGACATCTGGTGTATCTAACACTC
	ATTGATCAACAAGGCAACATCTTGAAGCAAGAATCATTGAACACTATCACAGACTCCAAG
	ATCTCGACTCCATATCACAAACTCCTTGACAATAAAGAAAACGAAAGGGATCTTGCCAGA
	AAAAATTGGGGTACAGTTGAAAATATTAAGGAACTAAAAGAAGGTTACATTTCGCAAGTA
	GTTCACAAGATTGCAACACTCATGTTGGAAGAAAACGCAATCGTTGTCATGGAAGATTTA
	AATTTCGGATTTAAGAGAGGAAGATTTAAAGTAGAAAAGCAAATCTACCAGAAGTTGGAG
	AAGATGTTAATTGACAAATTGAACTACTTAGTGCTGAAAGACAAACAGCCTCAAGAATTG
	GGCGGTCTATACAACGCTTTACAACTGACAAATAAATTTGAGTCATTCCAAAAGATGGGT
	AAGCAGAGTGGTTTTTTGTTTTATGTTCCGGCATGGAACACATCCAAAATCGATCCAACT
	ACAGGCTTCGTGAATTATTTCTACACTAAATATGAAAATGTGGATAAAGCAAAAGCTTTC
	TTTGAGAAGTTCGAGGCGATCCGTTTTAACGCTGAAAAGAAGTACTTCGAGTTCGAGGTC
	AAAAAGTATTCAGATTTTAACCCCAAGGCTGAAGGCACCCAGCAAGCATGGACTATTTGC
	ACGTACGGTGAGCGAATCGAAACTAAAAGGCAAAAGGATCAAAATAATAAGTTTGTAAGC
	ACACCCATTAACTTGACAGAAAAGATAGAAGATTTTCTTGGAAAAAACCAAATTGTATAT
	GGTGACGGTAACTGTATCAAGTCACAAATTGCTTCTAAAGACGATAAGGCCTTCTTCGAA
	ACTCTGCTATACTGGTTTAAAATGACGTTGCAAATGAGAAACAGTGAAACTAGAACTGAT
	ATCGACTATTTAATATCACCCGTGATGAACGATAATGGTACCTTTTACAATTCAAGAGAT
	TACGAGAAATTGGAGAACCCCACACTACCAAAAGACGCAGACGCTAATGGTGCCTACCAT
	ATTGCTAAAAAGGGACTGATGTTGTTGAACAAGATAGATCAAGCCGACTTAACTAAAAAA
	GTTGATTTGTCAATTTCGAATAGAGATTGGTTGCAATTCGTCCAGAAAAATAAGTAA

SEQ	ATGGAACAGGAATACTACTTGGGTTTGGATATGGGAACTGGTTCAGTCGGTTGGGCTGTT
ID	ACGGACTCCGAGTACCACGTGTTGAGAAAACACGGAAAGGCTTTATGGGGTGTCAGACTA
NO:	TTCGAATCAGCATCGACCGCGGAAGAGAGAAGAATGTTTAGAACTTCAAGAAGAAGGCTG
135	GATCGTAGGAATTGGCGGATAGAAATTTTACAAGAAATATTCGCCGAAGAAATCTCTAAA
	AAAGATCCAGGATTTTTTCTACGTATGAAGGAATCCAAATACTATCCGGAAGATAAACGT
	GATATTAATGGCAATTGTCCAGAGTTACCCTATGCTTTATTTGTGGACGACGATTTCACC
	GATAAAGATTACCATAAGAAGTTCCCAACAATTTACCATCTGAGAAAGATGTTAATGAAC
	ACTGAAGAAACCCCGGATATAAGACTGGTCTATCTAGCCATTCATCATATGATGAAACAC
	AGGGGACACTTCTTGCTATCAGGGGATATAAATGAAATTAAAGAATTTGGTACAACATTT
	TCTAAATTATTGGAAAATATTAAAAACGAAGAATTAGATTGGAATTTAGAATTAGGCAAG
	GAGGAATACGCAGTTGTCGAATCGATTCTGAAAGATAACATGTTGAACAGATCAACGAAA
	AAAACAAGGCTGATCAAGGCTTTAAAAGCGAAATCAATATGCGAAAAAGCAGTATTGAAT
	TTGTTAGCTGGGGGGACTGTCAAGTTGTCTGATATTTTCGGATTGGAAGAATTGAATGAA
	ACAGAGAGACCGAAGATATCCTTCGCCGATAATGGCTACGATGATTATATAGGCGAAGTC
	GAAAATGAGCTGGGCGAACAATTCTACATTATCGAGACTGCCAAGGCTGTTTATGATTGG
	GCGGTGTTAGTCGAAATCCTTGGCAAATACACTTCCATCTCCGAAGCTAAGGTGGCAACC
	TACGAAAAGCATAAAAGTGATTTGCAATTCCTTAAGAAAATTGTCCGAAAGTACTTGACC
	AAAGAAGAGTACAAGGATATTTTCGTATCAACATCGGACAAACTGAAGAATTATTCAGCT
	TATATTGGCATGACGAAAATTAATGGTAAGAAAGTTGATTTGCAATCCAAGAGATGTTCT
	AAAGAAGAATTTTACGATTTCATTAAAAAAAATGTCCTAAAAAAGTTGGAGGGACAACCT
	GAATATGAGTATTTAAAGGAAGAACTGGAAAGAGAAACTTTCCTACCAAAGCAAGTTAAT
	CGTGATAATGGCGTTATTCCATACCAAATACACTTGTACGAATTAAAGAAGATCTTGGGT
	AACTTGAGGGACAAAATTGATTTAATCAAGGAAAATGAAGACAAACTGGTACAATTATTT
	GAATTTAGAATACCTTACTACGTGGGCCCTTTAAACAAAATAGACGATGGTAAGGAAGGG
	AAGTTCACATGGGCAGTCAGAAAGTCCAATGAAAAAATTTACCCATGGAATTTCGAAAAC
	GTTGTAGATATTGAAGCTTCTGCTGAGAAATTTATTAGGAGAATGACAAATAAATGCACT
	TATCTTATGGGGGAAGACGTGTTGCCTAAAGATAGTTTATTATATTCAAAGTATATGGTC
	TTAAATGAATTAAACAATGTTAAATTAGATGGTGAAAAACTTTCCGTCGAATTGAAACAA
	AGATTGTATACAGATGTATTCTGCAAATATAGAAAAGTAACTGTAAAGAAGATTAAAAAC
	TACCTTAAATGTGAAGGCATTATCAGCGGAAATGTTGAGATCACTGGTATCGATGGTGAT
	TTTAAGGCATCTTTAACCGCATATCACGACTTTAAGGAAATATTGACGGGTACTGAGCTT
	GCTAAAAAAGACAAAGAGAACATTATCACCAATATCGTGCTCTTCGGAGACGACAAGAAA
	TTATTGAAAAAGAGATTGAACCGCCTATACCCTCAGATTACCCCTAACCAATTGAAGAAA
	ATCTGCGCTCTGTCTTATACTGGATGGGGTCGTTTTAGCAAGAAGTTTCTAGAAGAAATT
	ACTGCTCCGGATCCTGAAACTGGGGAAGTCTGGAATATAATTACCGCGCTATGGGAATCG
	AATAATAATTTAATGCAATTACTATCTAATGAATACAGATTTATGGAAGAAGTCGAAACT
	TACAATATGGGAAAACAAACAAAAACTTTGAGCTACGAAACAGTAGAGAATATGTATGTC
	TCACCATCTGTAAAGCGGCAGATCTGGCAAACCTTGAAGATAGTTAAAGAATTAGAAAAA
	GTGATGAAGGAAAGTCCAAAAAGGGTTTTTATTGAAATGGCCCGAGAAAAACAAGAATCT
	AAAAGGACGGAAAGTAGGAAAAAGCAACTTATAGATCTATATAAAGCCTGCAAAAATGAA
	GAAAAAGATTGGGTAAAGGAATTAGGTGACCAGGAAGAGCAAAAATTGAGATCTGACAAG
	CTGTACTTGTATTATACGCAAAAGGGCCGGTGTATGTATTCGGGTGAGGTAATAGAATTG
	AAAGATTTATGGGATAACACTAAGTATGACATTGACCATATTTACCCCCAGTCTAAGACA
	ATGGACGATTCATTAAATAACCGAGTTCTTGTCAAAAAGAAGTACAATGCCACAAAGAGC
	GATAAGTACCCATTGAACGAAAATATAAGACATGAACGAAAAGGTTTCTGGAAATCATTG
	TTGGACGGTGGATTTATTTCCAAAGAAAAATACGAGAGATTGATTAGAAACACTGAACTA
	TCTCCAGAGGAGTTAGCTGGCTTTATCGAAAGACAAATTGTTGAAACTAGACAGTCTACA
	AAAGCAGTTGCAGAAATCTTAAAACAAGTATTTCCAGAATCCGAAATTGTGTACGTCAAA
	GCCGGAACAGTAAGTAGATTTAGAAAAGACTTTGAATTATTGAAAGTACGAGAGGTTAAC
	GACCTACATCATGCTAAGGATGCTTATTTAAATATAGTCGTTGGTAATTCGTATTACGTG
	AAATTCACAAAAAACGCATCTTGGTTCATCAAGGAGAATCCTGGTAGGACATACAACTTG
	AAAAAGATGTTTACATCAGGATGGAATATCGAAAGAAATGGTGAGGTTGCGTGGGAGGTA
	GGCAAGAAGGGAACCATTGTTACTGTAAAGCAAATTATGAATAAAAACAATATACTTGTT
	ACGAGACAGGTGCACGAAGCCAAAGGAGGGTTGTTTGACCAGCAAATCATGAAGAAAGGT
	AAAGGTCAGATAGCAATAAAAGAGACTGATGAGCGTTTAGCTAGTATAGAAAAATATGGG
	GGCTACAATAAGGCAGCTGGTGCTTACTTCATGTTGGTCGAATCAAAGGATAAAAAAGGG
	AAGACGATCCGGACCATAGAGTTTATCCCTCTGTACTTGAAGAATAAGATTGAGTCTGAC
	GAAAGCATCGCATTGAATTTCTTGGAAAAGGGGCGCGGTCTAAAGGAGCCAAAAATATTG
	TTAAAGAAAATTAAAATAGACACCCTATTCGACGTCGATGGGTTTAAGATGTGGCTTAGT
	GGTCGTACTGGGGACAGATTATTATTCAAGTGTGCCAATCAGTTAATCCTTGACGAGAAA
	ATCATTGTTACAATGAAAAAAATTGTTAAGTTTATTCAAAGGCGACAAGAAAATAGAGAA
	CTAAAGTTGAGTGATAAGGATGGAATCGATAATGAAGTGTTAATGGAGATTTATAACACT
	TTTGTCGACAAATTGGAGAATACGGTGTACAGAATTAGGCTATCTGAACAGGCTAAAACC
	CTAATTGATAAACAGAAGGAGTTTGAGCGACTTTCTCTTGAAGACAAATCTTCAACTCTT
	TTCGAGATCCTACATATCTTTCAGTGTCAATCTTCTGCAGCTAATTTGAAAATGATTGGA
	GGTCCTGGTAAGGCTGGTATATTAGTCATGAACAACAACATATCTAAGTGTAATAAGATT
	AGTATAATTAACCAATCACCGACAGGTATCTTTGAAAATGAAATTGATTTACTTAAA

SEQ	ATGAAATCATTCGACTCGTTCACCAACTTGTACTCCCTGTCTAAAACATTGAAATTTGAA
ID	ATGCGACCTGTTGGTAACACCCAAAAGATGTTAGATAATGCAGGAGTTTTCGAAAAGGAT
NO:	AAACTGATCCAGAAAAAATACGGTAAAACGAAACCATATTTCGATAGGTTGCATCGGGAA
136	TTTATAGAAGAAGCTTTGACTGGTGTAGAATTAATTGGCTTAGATGAGAATTTCCGTACT
	CTAGTCGATTGGCAAAAAGATAAAAAGAACAATGTTGCCATGAAGGCATACGAAAATAGT
	CTACAAAGACTAAGAACAGAGATCGGGAAAATTTTCAATTTGAAGGCAGAAGACTGGGTG
	AAGAACAAATATCCAATATTGGGTCTTAAGAATAAGAATACTGATATATTGTTCGAGGAG
	GCCGTTTTCGGTATTCTTAAGGCAAGATATGGTGAAGAGAAAGACACGTTTATTGAAGTT
	GAGGAGATTGATAAAACCGGTAAGTCCAAAATCAACCAGATCTCTATCTTCGACAGTTGG
	AAGGGCTTCACTGGTTATTTTAAGAAGTTCTTCGAAACTAGGAAGAACTTCTATAAAAAC
	GATGGTACTTCCACGGCTATTGCTACAAGAATTATCGACCAAAACCTTAAGCGTTTTATT
	GATAACCTATCAATTGTTGAAAGTGTTCGACAGAAAGTAGATTTGGCTGAAACTGAAAAA
	TCTTTTAGTATCTCCTTATCCCAGTTTTTCTCTATAGATTTTTATAATAAATGTTTGCTG
	CAAGATGGCATTGACTACTATAATAAAATAATTGGTGGAGAGACATTGAAAAACGGAGAG
	AAGCTGATTGGCCTTAATGAGTTGATAAATCAATATAGACAAAATAATAAGGACCAGAAA
	ATCCCTTTCTTTAAATTGCTAGACAAACAGATTTTGTCTGAAAAGATCCTATTCTTGGAT
	GAAATAAAGAACGATACTGAATTGATTGAAGCTTTGTCCCAGTTTGCTAAAACAGCTGAA
	GAAAAGACAAAGATTGTGAAAAAATTGTTTGCTGATTTCGTAGAAAACAATTCTAAATAT
	GATCTAGCCCAGATTTATATAAGTCAAGAAGCTTTCAATACAATAAGTAATAAGTGGACA
	AGTGAAACAGAAACTTTTGCTAAGTATTTATTCGAAGCCATGAAGTCTGGTAAACTTGCC
	AAATACGAAAAAAAAGATAACAGTTATAAATTTCCAGACTTTATAGCCCTTTCACAGATG
	AAGTCTGCCTTATTGTCGATATCCTTAGAAGGTCATTTTTGGAAGGAAAAATATTATAAG
	ATAAGCAAGTTCCAAGAAAAGACTAATTGGGAACAATTTTTGGCTATATTTCTATATGAG
	TTCAATTCATTATTTTCCGATAAAATCAACACTAAGGATGGAGAGACTAAGCAAGTTGGC
	TACTATTTGTTCGCAAAAGATCTGCACAATTTGATTCTATCAGAACAAATAGATATACCA
	AAAGATTCAAAGGTAACTATAAAGGATTTCGCAGATTCCGTCCTCACCATTTATCAAATG
	GCTAAATATTTTGCCGTTGAAAAAAAGAGAGCGTGGTTAGCAGAATACGAGTTGGACTCG
	TTTTATACTCAGCCAGATACTGGATACTTGCAATTCTACGATAATGCATACGAAGACATT
	GTACAGGTATACAATAAACTTAGAAATTACTTAACCAAGAAGCCCTACAGTGAAGAAAAA
	TGGAAGCTGAACTTTGAAAATTCGACTTTGGCAAATGGTTGGGATAAAAATAAAGAAAGT
	GACAACTCCGCAGTGATTTTGCAAAAGGGTGGGAAATATTACTTGGGTTTAATCACAAAA
	GGCCACAATAAGATTTTTGATGATAGATTTCAAGAAAAATTCATAGTTGGTATAGAAGGT
	GGCAAATACGAGAAAATTGTCTATAAATTCTTCCCTGATCAAGCCAAAATGTTCCCAAAA
	GTTTGCTTTTCTGCTAAAGGATTGGAGTTTTTCCGGCCTAGCGAGGAGATCCTTCGTATC
	TACAACAATGCTGAATTCAAAAAAGGAGAAACCTATAGCATAGATTCTATGCAAAAACTG
	ATAGATTTTTATAAGGATTGTTTAACAAAGTACGAAGGCTGGGCCTGCTATACATTTAGA
	CATTTAAAGCCCACAGAAGAATACCAAAATAACATTGGTGAATTCTTTCGGGACGTTGCC
	GAAGACGGCTATAGGATCGATTTTCAAGGTATCTCAGATCAATATATCCACGAAAAGAAC
	GAGAAGGGTGAGCTGCACCTTTTCGAAATTCATAATAAGGACTGGAATTTGGATAAGGCG
	AGAGATGGTAAATCGAAGACCACTCAAAAGAACTTGCATACTTTATATTTTGAGTCCTTG
	TTTTCTAATGATAACGTCGTCCAAAATTTTCCAATAAAGTTGAATGGACAAGCGGAAATT
	TTCTATCGGCCTAAGACAGAGAAAGACAAATTAGAATCAAAGAAAGATAAAAAGGGAAAT
	AAAGTCATTGATCACAAACGATACTCTGAGAATAAAATATTTTTCCACGTACCATTGACA
	CTCAACAGGACTAAGAATGACTCTTATAGATTTAATGCTCAGATTAATAATTTTTTGGCA
	AATAACAAGGATATTAACATAATTGGGGTGGATAGAGGTGAAAAGCACTTGGTATATTAC
	TCTGTCATCACTCAGGCTTCTGATATATTGGAAAGCGGGTCTCTAAATGAATTGAACGGT
	GTTAACTACGCCGAAAAGCTAGGTAAAAAAGCTGAAAACAGAGAGCAGGCTCGGCGCGAT
	TGGCAAGATGTTCAAGGAATTAAAGACCTTAAAAAAGGCTACATTAGTCAAGTAGTTAGA
	AAGTTAGCCGATCTTGCTATTAAACATAACGCAATCATTATTCTGGAGGACCTAAATATG
	CGTTTTAAGCAAGTTAGGGGTGGCATAGAAAAAAGTATTTATCAGCAGCTTGAGAAGGCT
	TTGATAGATAAGTTATCGTTCCTAGTTGACAAAGGTGAAAAAAATCCTGAACAAGCTGGT
	CATCTGTTGAAAGCTTATCAGCTGAGCGCACCTTTTGAAACATTTCAAAAAATGGGAAAA
	CAAACAGGTATTATTTTCTATACTCAAGCGAGTTATACAAGTAAATCTGACCCAGTGACA
	GGATGGAGACCACACCTTTATCTAAAATATTTTTCTGCTAAAAAGGCCAAAGATGACATC
	GCTAAGTTTACAAAAATAGAATTTGTCAACGATAGATTTGAATTGACTTACGATATTAAA
	GATTTTCAGCAAGCAAAAGAATACCCAAATAAGACAGTGTGGAAAGTATGCTCCAATGTG
	GAGAGATTTAGATGGGATAAAAATCTCAATCAAAACAAGGGTGGTTACACACATTATACT
	AATATAACTGAAAATATTCAAGAATTGTTTACTAAGTACGGAATTGACATAACCAAAGAC
	TTACTAACTCAGATTTCAACTATTGACGAAAAACAAAATACCTCATTTTTCCGCGACTTT
	ATTTTTTATTTCAACTTGATCTGTCAAATTCGTAACACGGATGATTCCGAAATTGCCAAG
	AAGAACGGAAAAGATGATTTCATCCTATCTCCAGTGGAACCATTTTTTGACTCAAGAAAA
	GATAATGGTAATAAGTTGCCTGAGAACGGAGATGATAACGGCGCTTATAATATCGCTCGG
	AAGGGTATTGTAATTCTTAATAAAATATCTCAGTACTCTGAAAAGAACGAAAACTGCGAG
	AAAATGAAGTGGGGCGACTTGTATGTATCTAATATAGATTGGGATAATTTCGTTACTCAA
	GCCAACGCGAGACATTGA

SEQ	ATGGAAAATTTTAAAAACCTATATCCAATTAATAAGACACTTAGATTCGAGCTTAGGCCA
ID	TACGGCAAAACACTAGAAAATTTTAAGAAGTCAGGCCTATTAGAAAAAGACGCCTTTAAG
NO:	GCAAATTCCAGAAGATCAATGCAGGCAATTATTGATGAGAAATTTAAAGAGACTATCGAG
137	GAAAGGTTGAAATACACTGAATTCTCTGAGTGCGATCTGGGAAACATGACTTCCAAGGAT
	AAAAAGATTACCGATAAGGCTGCTACCAACCTCAAAAAGCAAGTCATCTTATCGTTTGAT
	GATGAAATTTTTAATAACTACTTAAAGCCGGACAAAAACATTGACGCCCTATTCAAAAAT
	GATCCGTCCAACCCCGTAATTTCAACTTTTAAGGGTTTTACCACGTACTTTGTAAATTTT
	TTTGAGATTCGTAAACATATCTTCAAAGGAGAATCGTCGGGTTCCATGGCCTATAGGATA
	ATTGATGAAAATCTTACGACTTACTTAAACAATATCGAAAAGATAAAAAAGTTACCAGAA
	GAATTAAAGTCTCAATTGGAAGGTATTGACCAAATAGACAAATTAAATAACTATAATGAG
	TTCATAACTCAAAGCGGTATCACACATTACAATGAAATTATCGGTGGTATATCTAAAAGT
	GAGAACGTAAAAATACAGGGAATAAACGAGGGGATCAATCTATACTGTCAGAAGAATAAA
	GTAAAATTACCAAGACTAACGCCATTATACAAAATGATTCTGTCTGATAGAGTTTCCAAC
	TCGTTCGTGCTTGATACTATAGAAAATGATACTGAATTAATTGAGATGATTAGCGACTTG
	ATTAATAAAACAGAAATATCTCAAGACGTAATAATGTCAGACATTCAGAACATTTTCATA
	AAATATAAACAGCTTGGTAATTTACCGGGGATAAGTTACTCTAGCATCGTGAATGCTATT
	TGCTCCGATTATGACAATAATTTTGGTGACGGAAAAAGAAAAAAATCATATGAGAACGAT
	AGGAAGAAACACCTTGAAACAAACGTATACTCAATTAACTATATATCGGAACTGTTAACA
	GACACCGATGTATCATCTAATATAAAAATGAGATATAAGGAACTTGAACAAAATTACCAG
	GTGTGTAAGGAGAATTTCAATGCTACCAACTGGATGAACATTAAGAATATTAAACAGAGT
	GAAAAGACAAACTTGATTAAAGATCTACTAGATATACTGAAATCAATACAGAGATTCTAC
	GATCTGTTTGATATAGTTGATGAAGACAAAAATCCTAGTGCTGAGTTTTACACGTGGCTA
	AGTAAAAATGCGGAAAAGTTAGATTTCGAGTTCAACTCTGTTTATAATAAATCTAGGAAT
	TATTTAACTAGAAAGCAGTATTCTGATAAAAAGATAAAATTGAACTTCGACTCCCCTACG
	TTGGCAAAGGGTTGGGATGCAAACAAAGAAATCGATAACTCCACCATAATAATGCGTAAG
	TTTAACAATGATAGGGGGGATTACGATTATTTTTTGGGAATTTGGAACAAATCTACCCCA
	GCGAATGAAAAAATTATTCCCCTTGAAGACAATGGTCTTTTTGAAAAAATGCAGTATAAA
	TTATATCCAGACCCATCCAAGATGCTTCCAAAGCAATTTCTGTCAAAAATTTGGAAGGCT
	AAACACCCTACTACTCCTGAATTTGATAAGAAGTATAAGGAGGGCCGACACAAAAAGGGT
	CCAGATTTTGAAAAAGAATTCCTGCATGAATTGATAGATTGTTTTAAGCATGGTTTGGTA
	AATCATGATGAAAAATATCAGGATGTCTTTGGATTCAATTTGAGAAATACAGAGGATTAC
	AACTCATATACAGAATTTCTCGAGGACGTCGAACGTTGCAATTATAATCTCAGTTTCAAC
	AAGATCGCAGACACTTCAAACTTAATTAACGACGGAAAATTGTACGTTTTTCAAATCTGG
	TCGAAAGACTTTAGTATTGATTCAAAGGGTACAAAAAACCTAAATACAATATATTTCGAA
	AGTCTATTCTCGGAAGAAAACATGATCGAAAAAATGTTCAAACTGTCAGGCGAAGCTGAA
	ATATTCTACCGTCCCGCAAGCCTTAATTATTGTGAGGATATCATTAAAAAAGGACATCAC
	CATGCAGAGTTAAAAGATAAATTCGATTACCCAATAATTAAAGATAAAAGATACTCCCAG
	GATAAGTTCTTTTTCCATGTACCTATGGTTATTAACTACAAGTCGGAAAAACTAAACTCG
	AAGTCATTAAATAATAGAACTAACGAGAACTTGGGACAATTCACACATATAATTGGTATT
	GATCGTGGCGAAAGACATTTAATATATCTGACTGTTGTTGATGTTTCAACAGGAGAAATT
	GTTGAACAGAAACATCTTGATGAAATTATAAACACAGATACAAAAGGCGTTGAGCATAAA
	ACTCATTATCTAAATAAATTGGAGGAAAAGTCGAAGACTCGCGATAACGAGAGAAAGAGT
	TGGGAAGCAATTGAAACCATAAAAGAGCTTAAAGAAGGTTACATTAGTCACGTCATCAAT
	GAAATACAAAAGTTACAAGAAAAGTATAACGCTTTGATTGTAATGGAAAATCTAAATTAT
	GGTTTTAAGAATTCAAGAATCAAAGTCGAAAAGCAGGTCTATCAGAAATTTGAAACGGCA
	CTTATTAAAAAGTTTAACTACATTATTGATAAAAAGGACCCAGAAACTTATATTCATGGT
	TACCAACTGACGAACCCAATCACAACATTGGACAAAATTGGAAACCAAAGTGGAATTGTT
	TTATACATTCCAGCTTGGAATACATCCAAAATAGACCCTGTCACGGGGTTTGTCAACTTG
	TTATATGCCGACGATTTAAAGTATAAAAACCAAGAACAAGCAAAGTCTTTTATTCAAAAG
	ATTGATAATATTTATTTCGAAAACGGTGAATTTAAATTCGACATAGATTTTTCTAAATGG
	AACAACCGTTATTCAATAAGTAAAACTAAATGGACACTCACCTCATACGGCACTCGTATC
	CAAACCTTTCGGAATCCCCAAAAAAATAACAAATGGGATTCTGCAGAATACGACTTGACC
	GAGGAATTTAAATTAATTCTTAATATAGACGGTACACTCAAAAGTCAAGACGTGGAGACA
	TACAAGAAGTTTATGTCGTTATTCAAGCTTATGCTTCAGTTGAGGAACTCCGTTACAGGC
	ACTGATATTGATTACATGATTTCACCAGTAACGGATAAGACTGGGACTCATTTCGATTCT
	AGGGAAAATATTAAAAATTTACCTGCTGACGCAGACGCAAACGGCGCATACAATATAGCA
	AGAAAAGGGATTATGGCCATTGAGAATATTATGAATGGCATATCAGATCCATTAAAGATA
	AGCAATGAAGACTACTTAAAATACATTCAGAATCAGCAAGAATAA

SEQ	ATGACCCAGTTTGAAGGTTTCACCAATTTGTACCAAGTAAGTAAAACCTTGAGGTTCGAA
ID	TTGATCCCACAGGGCAAGACATTGAAGCATATTCAAGAGCAAGGATTTATAGAAGAAGAT
NO:	AAAGCGAGAAACGATCACTATAAAGAGTTAAAACCCATTATTGACAGGATCTATAAAACA
138	TACGCCGATCAATGCCTTCAATTAGTGCAATTAGATTGGGAAAACTTGAGCGCTGCCATC
	GATTCCTACAGGAAGGAAAAAACAGAAGAAACAAGAAATGCCTTAATCGAGGAACAAGCA
	ACCTATAGAAACGCTATACACGATTACTTCATCGGTAGAACTGATAATCTAACAGATGCA
	ATAAATAAGAGACATGCTGAGATATATAAAGGACTATTTAAAGCAGAATTATTCAACGGA
	AAGGTGTTGAAACAGTTAGGTACCGTTACAACTACTGAGCATGAAAATGCCTTGCTGAGA
	AGCTTTGACAAGTTTACTACCTACTTTTCGGGTTTCTACGAAAATCGCAAAAATGTATTT
	TCTGCGGAAGATATTTCAACTGCAATCCCTCATAGGATTGTTCAAGATAATTTCCCTAAG
	TTTAAAGAGAACTGTCACATTTTTACAAGGTTAATTACTGCGGTTCCAAGTCTAAGAGAA
	CATTTTGAGAATGTAAAAAAAGCGATTGGTATATTTGTATCCACTAGCATTGAAGAGGTT
	TTCAGCTTCCCTTTTTATAACCAATTACTTACCCAAACACAGATCGACCTGTACAACCAA
	TTGTTAGGTGGTATATCGAGGGAGGCTGGTACGGAAAAGATTAAAGGATTAAATGAAGTT
	CTTAATTTGGCCATACAAAAAAATGATGAAACCGCGCACATTATCGCATCTTTACCACAT
	AGGTTTATACCGTTATTCAAGCAAATATTATCTGATCGTAATACCTTATCGTTCATATTA
	GAGGAGTTTAAATCTGACGAAGAAGTTATACAATCTTTTTGCAAGTATAAGACGCTATTG
	AGAAACGAAAACGTTCTGGAAACAGCCGAAGCACTGTTCAATGAATTAAACAGTATCGAC
	TTGACTCATATTTTTATATCGCATAAAAAGTTGGAGACAATTTCTTCAGCATTGTGCGAT
	CACTGGGACACTTTAAGGAACGCACTATATGAACGTAGGATCTCAGAATTGACAGGTAAG
	ATAACGAAGTCTGCTAAAGAGAAAGTGCAGAGATCCCTAAAACACGAGGATATAAATTTG
	CAGGAGATAATTTCAGCTGCAGGTAAAGAGTTGTCTGAAGCGTTCAAGCAAAAGACTTCC
	GAAATCTTGTCACACGCACACGCCGCATTAGATCAACCTTTACCCACTACTTTGAAAAAA
	CAAGAAGAGAAGGAGATATTAAAATCACAACTTGATTCTTTACTTGGCCTTTATCATCTT
	TTAGATTGGTTCGCTGTTGACGAGAGCAATGAAGTGGATCCAGAGTTTTCCGCAAGATTG
	ACCGGTATAAAGTTGGAAATGGAACCTTCGTTATCATTTTACAACAAAGCTAGGAACTAT
	GCTACAAAAAAACCTTATTCTGTCGAAAAATTTAAACTGAACTTCCAAATGCCTACTCTA
	GCAAGTGGCTGGGATGTTAATAAAGAAAAGAACAATGGCGCTATTTTGTTTGTAAAAAAT
	GGCCTATACTATCTTGGAATTATGCCTAAACAAAAAGGTCGCTACAAGGCTTTGTCATTT
	GAACCTACTGAAAAGACTAGCGAAGGTTTCGATAAGATGTATTACGATTATTTCCCGGAT
	GCCGCTAAAATGATCCCCAAGTGCTCTACTCAATTGAAGGCAGTAACTGCTCATTTCCAA
	ACGCATACCACGCCAATACTGCTTTCTAACAACTTTATAGAACCACTAGAAATAACGAAA
	GAAATTTACGACCTAAATAACCCAGAGAAAGAACCAAAAAAGTTCCAGACGGCCTACGCC
	AAAAAGACAGGGGACCAAAAAGGTTACCGCGAGGCGTTATGTAAATGGATTGATTTTACT
	AGGGACTTTTTATCAAAATACACTAAAACGACGTCTATTGATCTTAGCTCCTTACGCCCG
	TCCTCCCAATACAAGGATCTAGGTGAGTATTACGCAGAGTTGAACCCGCTATTATACCAT
	ATTTCCTTCCAAAGGATTGCTGAAAAGGAAATTATGGACGCTGTTGAAACTGGGAAATTG
	TACCTGTTTCAGATTTATAATAAGGACTTCGCAAAGGGTCACCATGGTAAGCCTAACCTT
	CACACTTTGTACTGGACCGGACTATTCTCGCCTGAAAATTTGGCTAAAACAAGTATCAAG
	TTAAACGGTCAGGCCGAGTTATTTTATAGACCCAAATCTAGAATGAAAAGAATGGCCCAT
	AGATTAGGCGAAAAGATGTTAAACAAGAAATTAAAGGACCAAAAAACCCCGATACCAGAC
	ACTCTATACCAAGAACTGTACGACTATGTGAATCACAGGCTTAGTCACGATTTATCAGAT
	GAAGCGAGGGCTTTATTGCCAAATGTCATCACCAAGGAAGTATCACATGAAATAATTAAG
	GATAGAAGGTTCACATCTGATAAATTCTTTTTTCATGTCCCAATTACATTGAATTATCAA
	GCAGCGAACTCACCATCTAAATTTAATCAGCGCGTCAACGCCTATTTGAAAGAACATCCC
	GAAACACCAATCATCGGCATAGATCGAGGTGAGAGAAACTTAATATATATAACTGTGATT
	GATTCTACAGGAAAAATCCTGGAGCAACGATCTTTAAATACCATACAACAGTTTGATTAT
	CAAAAAAAGTTGGATAACAGAGAAAAAGAACGTGTTGCCGCTAGGCAGGCTTGGTCTGTG
	GTAGGAACAATTAAGGACTTAAAGCAGGGCTATCTGTCCCAAGTTATTCATGAAATAGTC
	GATCTGATGATACATTATCAGGCAGTTGTCGTGTTGGAAAATTTGAATTTTGGCTTTAAA
	TCAAAAAGAACTGGCATAGCAGAAAAAGCTGTGTACCAGCAGTTTGAAAAGATGTTAATC
	GATAAGCTAAACTGCCTTGTTCTTAAAGATTACCCCGCAGAAAAAGTAGGTGGTGTTCTT
	AATCCATATCAGTTGACAGACCAATTTACATCCTTTGCGAAAATGGGTACGCAAAGCGGG
	TTCTTATTCTACGTACCGGCCCCCTATACTTCTAAGATCGACCCACTAACAGGTTTTGTG
	GACCCTTTTGTTTGGAAGACGATAAAGAACCACGAGTCACGCAAACATTTCTTAGAGGGC
	TTTGATTTCTTGCACTACGACGTGAAAACTGGTGATTTTATCTTACACTTTAAAATGAAC
	AGAAATCTCTCTTTCCAACGTGGACTGCCCGGATTCATGCCGGCTTGGGACATCGTTTTT
	GAAAAGAATGAAACGCAGTTTGACGCCAAAGGTACACCATTTATAGCGGGTAAGAGAATT
	GTGCCGGTCATAGAAAACCATAGATTTACAGGTAGATATAGGGATCTGTACCCTGCTAAT
	GAATTGATTGCATTACTCGAAGAGAAAGGAATTGTGTTTCGAGATGGATCGAATATTTTA
	CCTAAGTTGTTGGAAAATGATGATTCACACGCAATTGATACTATGGTTGCCCTCATAAGA
	TCGGTATTGCAAATGAGAAACTCAAATGCTGCTACGGGAGAGGATTATATAAACAGCCCC
	GTTCGCGATCTTAATGGTGTTTGTTTTGATTCACGTTTTCAGAACCCCGAATGGCCAATG
	GATGCCGACGCAAACGGAGCATATCATATTGCTCTTAAAGGCCAACTACTATTAAATCAC
	TTAAAGGAATCCAAAGACCTAAAATTGCAAAACGGGATATCTAATCAGGATTGGCTGGCT
	TACATACAAGAACTACGTAACTAG

SEQ	ATGGCCGTTAAGTCAATCAAAGTGAAACTTAGACTGGATGACATGCCAGAGATTCGTGCG
ID	GGGTTATGGAAACTTCATAAGGAAGTTAACGCAGGGGTAAGATATTATACCGAATGGTTA
NO:	TCATTACTTCGACAAGAGAATTTGTACAGAAGGTCCCCGAACGGCGACGGTGAGCAAGAA
139	TGCGATAAGACGGCTGAAGAATGTAAGGCAGAACTTTTGGAGCGCCTGAGAGCCCGTCAG
	GTTGAAAATGGCCATAGAGGTCCTGCGGGATCTGATGATGAGCTTTTACAGCTAGCTAGA
	CAATTGTATGAATTGTTGGTCCCTCAGGCTATTGGGGCTAAAGGAGACGCTCAACAAATC
	GCCAGAAAGTTCTTGTCACCTCTGGCTGACAAAGATGCCGTGGGAGGATTAGGTATCGCT
	AAAGCAGGTAATAAACCAAGATGGGTTAGAATGAGAGAAGCAGGCGAACCTGGTTGGGAA
	GAAGAGAAAGAAAAGGCCGAAACTAGAAAAAGCGCTGACAGAACCGCAGATGTTTTACGG
	GCCTTGGCTGATTTTGGACTGAAGCCTTTGATGAGAGTGTATACTGATTCAGAAATGTCT
	TCCGTTGAATGGAAGCCCCTAAGGAAGGGACAAGCGGTCAGAACCTGGGATAGGGATATG
	TTTCAACAGGCTATTGAAAGGATGATGTCATGGGAATCCTGGAATCAAAGAGTAGGTCAA
	GAATACGCTAAACTGGTCGAACAAAAGAATAGATTTGAACAAAAAAATTTTGTAGGTCAA
	GAACATTTAGTACATTTGGTTAATCAACTTCAACAAGATATGAAAGAGGCATCTCCTGGT
	TTGGAATCAAAAGAACAAACAGCACACTATGTTACCGGCCGAGCTTTGCGAGGTTCTGAC
	AAAGTATTTGAAAAGTGGGGGAAATTAGCTCCCGATGCCCCCTTTGATCTATATGATGCT
	GAAATTAAAAACGTTCAAAGAAGGAACACTAGACGTTTTGGATCCCATGATCTTTTTGCA
	AAGCTAGCTGAGCCAGAATACCAGGCTCTATGGCGTGAAGACGCCTCGTTTTTGACTAGA
	TACGCAGTATACAATTCAATACTCAGAAAACTAAACCATGCCAAGATGTTTGCTACATTC
	ACCCTGCCCGATGCTACCGCTCATCCTATTTGGACTAGATTTGACAAGTTGGGGGGGAAT
	CTACATCAGTACACATTTTTATTTAATGAATTCGGTGAAAGAAGACACGCTATTAGATTC
	CACAAGCTCCTAAAGGTTGAAAACGGCGTTGCGAGAGAAGTTGATGATGTAACAGTTCCC
	ATTTCTATGTCGGAGCAATTGGATAATCTATTGCCTAGAGACCCTAATGAACCAATTGCT
	TTGTACTTTCGTGACTACGGTGCAGAACAACACTTTACAGGTGAATTCGGCGGAGCCAAG
	ATTCAATGTAGACGTGATCAACTCGCACACATGCATAGAAGAAGAGGCGCTCGTGATGTT
	TATTTAAATGTGTCTGTTAGAGTTCAATCCCAATCGGAGGCTAGAGGTGAAAGAAGGCCA
	CCATACGCAGCAGTTTTTAGGTTAGTAGGTGATAATCATAGGGCATTTGTCCACTTCGAC
	AAATTAAGTGATTATTTAGCAGAGCACCCTGATGATGGAAAGTTGGGCAGTGAGGGATTA
	TTAAGTGGGTTGAGGGTAATGTCTGTAGATCTTGGTCTTCGTACTTCTGCGAGTATCTCT
	GTCTTTAGAGTAGCACGTAAGGATGAGTTGAAACCTAATAGCAAAGGAAGAGTCCCGTTT
	TTTTTTCCTATTAAGGGTAACGATAACCTGGTGGCCGTGCATGAAAGATCACAACTTTTG
	AAATTGCCAGGAGAAACGGAGTCCAAGGACTTGAGGGCAATTAGAGAGGAACGTCAGCGT
	ACATTGCGACAGCTGAGAACTCAATTGGCTTATTTGAGGTTGTTGGTTAGGTGTGGTTCC
	GAGGATGTTGGCAGAAGAGAAAGGTCTTGGGCCAAATTGATAGAACAACCAGTGGACGCC
	GCAAATCACATGACACCAGATTGGAGAGAAGCTTTCGAAAATGAACTCCAGAAATTAAAG
	AGCCTACATGGCATATGCTCTGATAAAGAGTGGATGGATGCCGTATACGAATCCGTTCGT
	AGAGTCTGGCGCCACATGGGTAAGCAAGTACGGGACTGGAGAAAGGATGTTCGTTCCGGC
	GAAAGACCGAAGATAAGGGGGTATGCAAAGGACGTTGTAGGCGGTAATTCTATTGAACAG
	ATTGAGTATTTGGAAAGGCAGTACAAATTTCTTAAATCCTGGAGCTTCTTCGGCAAAGTG
	TCAGGACAAGTCATCAGGGCTGAAAAAGGTTCCAGATTTGCTATTACGCTAAGGGAACAT
	ATTGATCATGCGAAAGAAGATAGACTGAAAAAACTAGCAGATAGAATAATTATGGAAGCA
	CTTGGTTACGTCTATGCACTTGATGAAAGAGGCAAGGGGAAATGGGTAGCTAAATACCCG
	CCTTGTCAACTTATTTTATTAGAAGAATTAAGCGAGTACCAATTTAACAACGATAGACCT
	CCATCCGAAAATAATCAGCTGATGCAATGGTCCCATAGGGGTGTTTTTCAAGAATTGATA
	AATCAAGCTCAAGTACACGATTTGCTGGTAGGTACTATGTACGCAGCGTTTTCGAGCCGT
	TTTGATGCAAGAACTGGTGCCCCAGGTATCAGATGTCGACGTGTTCCGGCCAGATGTACA
	CAGGAACATAACCCTGAGCCATTTCCGTGGTGGCTTAATAAGTTTGTTGTCGAGCACACA
	TTAGACGCATGCCCTCTGAGAGCAGATGACCTTATACCCACTGGAGAAGGCGAAATATTT
	GTTAGTCCATTCTCTGCAGAAGAAGGTGACTTTCACCAGATACATGCAGACTTAAATGCA
	GCACAGAATCTCCAACAAAGGTTGTGGTCGGATTTTGATATTTCGCAAATAAGACTAAGA
	TGCGATTGGGGAGAGGTTGATGGAGAATTGGTGCTGATTCCAAGATTAACCGGAAAGCGA
	ACTGCCGATTCCTATTCTAACAAGGTGTTTTACACAAATACTGGTGTTACCTATTACGAA
	AGAGAAAGGGGTAAGAAGAGACGTAAAGTATTTGCTCAAGAAAAATTGTCAGAAGAGGAG
	GCAGAACTGTTAGTAGAAGCAGACGAAGCCAGAGAAAAATCAGTTGTGCTTATGCGTGAC
	CCTTCCGGCATTATAAATCGTGGTAATTGGACACGACAAAAAGAATTTTGGTCTATGGTC
	AATCAACGTATCGAAGGCTACCTAGTTAAGCAAATCAGGTCTAGGGTTCCACTACAAGAT
	AGCGCATGTGAAAATACGGGTGATATATAA

SEQ	ATGGCTACTAGATCTTTCATTTTAAAAATTGAACCTAATGAAGAAGTGAAGAAGGGTCTC
ID	TGGAAAACTCACGAAGTACTTAATCATGGCATTGCCTATTATATGAATATCCTGAAGCTT
NO:	ATTCGTCAAGAAGCTATATACGAGCATCATGAGCAAGATCCTAAGAACCCTAAGAAAGTA
140	AGCAAAGCGGAAATTCAGGCTGAATTGTGGGACTTCGTCTTGAAGATGCAGAAGTGTAAC
	AGTTTTACGCACGAAGTTGATAAAGATGTGGTGTTTAATATTTTGAGGGAGCTATATGAG
	GAGTTGGTGCCCTCGAGTGTCGAAAAAAAAGGAGAAGCTAATCAGCTGTCAAATAAATTT
	TTATATCCTCTGGTGGATCCAAACTCTCAATCAGGTAAAGGCACTGCCAGTAGTGGTCGA
	AAACCGAGATGGTATAATTTGAAAATCGCAGGTGATCCATCGTGGGAAGAAGAAAAAAAA
	AAATGGGAAGAAGATAAAAAAAAAGATCCCCTTGCCAAAATACTAGGTAAGCTAGCCGAG
	TATGGACTTATACCATTATTCATTCCTTTCACGGACTCTAATGAACCAATTGTGAAGGAA
	ATCAAATGGATGGAAAAATCACGTAATCAGTCTGTTAGGAGGTTGGACAAAGATATGTTT
	ATACAGGCTCTTGAGAGGTTTTTGTCGTGGGAGTCCTGGAATTTGAAAGTGAAAGAAGAA
	TATGAAAAAGTGGAAAAGGAGCATAAGACGTTGGAAGAAAGGATTAAGGAAGATATTCAG
	GCCTTTAAGAGTCTGGAACAGTACGAAAAAGAAAGACAGGAACAGTTATTGAGAGATACT
	CTAAACACTAATGAATATAGGCTTTCCAAGAGGGGCTTGCGAGGATGGAGAGAGATAATT
	CAGAAATGGTTGAAAATGGATGAGAACGAGCCATCGGAGAAATATCTAGAGGTGTTTAAA
	GATTACCAAAGAAAGCACCCTCGCGAAGCTGGTGATTACTCTGTTTATGAATTCCTTTCG
	AAGAAGGAAAATCACTTCATCTGGCGAAATCATCCAGAGTACCCATATTTATATGCTACA
	TTTTGCGAAATTGACAAGAAAAAAAAAGATGCTAAACAGCAAGCGACATTCACCCTCGCT
	GATCCCATCAACCACCCATTATGGGTCAGGTTCGAAGAGAGATCAGGCTCGAACCTGAAT
	AAGTACAGGATCTTGACTGAGCAATTGCATACTGAGAAGTTAAAAAAGAAATTGACGGTC
	CAACTTGACAGATTGATTTATCCCACTGAATCTGGTGGATGGGAGGAGAAAGGTAAGGTT
	GATATTGTCCTATTGCCTTCTCGTCAATTTTACAACCAAATATTTCTGGACATCGAAGAG
	AAGGGTAAACATGCTTTTACCTATAAGGATGAGAGTATTAAATTTCCATTGAAGGGAACG
	CTTGGCGGCGCTAGAGTTCAGTTCGATAGAGATCATTTGAGAAGATACCCGCATAAAGTG
	GAATCTGGTAATGTAGGTCGGATCTACTTTAACATGACGGTAAATATTGAACCTACCGAG
	TCACCAGTCAGTAAGTCTTTAAAGATTCATAGGGATGATTTCCCTAAATTTGTCAACTTC
	AAGCCTAAGGAACTAACCGAGTGGATCAAAGACAGTAAAGGCAAAAAGTTAAAGAGCGGT
	ATTGAGTCCCTGGAGATAGGTCTTAGAGTCATGTCTATCGATTTGGGTCAAAGACAAGCA
	GCCGCAGCATCTATTTTCGAAGTTGTTGACCAAAAACCGGATATCGAGGGGAAATTATTT
	TTTCCAATAAAAGGAACTGAGCTATACGCTGTGCATCGCGCATCCTTCAATATAAAACTG
	CCAGGAGAAACACTAGTAAAATCTAGAGAGGTCTTGCGTAAAGCACGTGAGGACAATCTC
	AAATTAATGAATCAGAAGTTAAATTTCCTTAGGAACGTGTTGCATTTCCAACAGTTCGAG
	GACATAACTGAACGCGAGAAAAGAGTCACTAAGTGGATCTCAAGACAAGAAAATAGTGAT
	GTGCCATTAGTGTATCAAGACGAACTTATTCAAATAAGAGAGCTAATGTATAAACCATAT
	AAAGACTGGGTGGCATTCTTAAAACAATTACACAAGCGGCTTGAAGTAGAAATAGGAAAA
	GAAGTAAAGCATTGGAGGAAGAGTCTGTCCGATGGTCGCAAAGGCCTGTACGGGATATCA
	CTTAAAAATATTGATGAAATTGACAGAACACGAAAATTTTTGTTAAGATGGTCATTGAGA
	CCAACCGAACCAGGTGAGGTTAGAAGGTTGGAACCAGGCCAAAGGTTTGCCATCGATCAA
	TTAAACCATCTTAACGCACTGAAAGAAGATAGATTGAAGAAGATGGCGAACACTATTATT
	ATGCACGCTCTAGGTTATTGCTATGATGTGAGAAAGAAAAAATGGCAAGCCAAGAACCCT
	GCATGCCAAATTATTTTGTTTGAAGATCTTTCTAATTACAATCCATACGAAGAGCGTTCA
	CGTTTTGAAAACTCTAAATTGATGAAATGGTCTAGAAGAGAGATTCCGAGACAGGTCGCT
	CTACAAGGGGAGATTTACGGTCTTCAAGTCGGTGAGGTTGGTGCTCAATTTTCTTCCAGA
	TTTCATGCAAAAACTGGGTCTCCAGGCATTAGGTGTTCGGTCGTTACTAAGGAAAAGTTA
	CAGGACAACCGTTTCTTCAAAAATTTGCAACGTGAAGGCCGTTTAACACTTGATAAGATA
	GCTGTCCTTAAGGAAGGCGATCTGTACCCAGATAAAGGTGGTGAGAAATTCATATCTTTG
	AGTAAAGACAGGAAACTGGTTACAACACACGCCGACATTAACGCAGCTCAGAACTTGCAA
	AAGAGATTCTGGACAAGGACCCACGGCTTCTATAAGGTGTACTGTAAAGCTTATCAAGTA
	GATGGACAAACGGTTTATATTCCTGAATCAAAGGACCAGAAACAAAAAATTATAGAAGAA
	TTTGGTGAAGGATACTTTATCTTGAAGGATGGAGTTTATGAGTGGGGCAATGCAGGTAAG
	TTAAAGATAAAGAAAGGTTCATCAAAGCAATCAAGTAGCGAACTGGTCGATTCGGATATT
	TTAAAGGATAGCTTTGATCTAGCTAGTGAATTGAAGGGAGAAAAGTTAATGTTATACAGA
	GATCCCAGTGGGAATGTATTTCCATCTGATAAGTGGATGGCCGCCGGAGTGTTTTTTGGC
	AAATTAGAGAGAATCTTGATTTCTAAACTGACCAATCAATACTCAATTTCGACCATCGAA
	GACGACTCTTCAAAACAATCCATGTGA

SEQ	ATGCCTACTCGCACCATCAATCTGAAGTTAGTTTTGGGGAAGAACCCAGAAAATGCGACT
ID	CTAAGACGGGCACTATTCTCTACACATAGACTTGTCAACCAAGCGACTAAGAGAATTGAA
NO:	GAATTTTTACTGTTGTGTAGAGGAGAAGCTTATCGTACCGTAGATAATGAAGGTAAAGAA
141	GCTGAGATCCCACGCCATGCTGTTCAAGAAGAGGCGCTTGCTTTTGCAAAAGCTGCACAA
	CGACATAACGGCTGTATCTCCACATATGAGGACCAGGAAATCTTGGATGTGCTTAGACAA
	TTGTATGAAAGATTAGTACCTAGCGTCAATGAAAACAACGAGGCTGGGGATGCCCAAGCC
	GCTAACGCTTGGGTGAGTCCATTAATGAGTGCAGAGTCCGAAGGTGGACTATCGGTCTAT
	GATAAAGTGTTAGACCCGCCGCCAGTATGGATGAAACTCAAAGAAGAGAAAGCGCCTGGT
	TGGGAAGCTGCTTCTCAGATTTGGATACAGTCCGACGAAGGTCAATCGCTGCTAAATAAA
	CCGGGTAGCCCACCACGTTGGATTAGAAAACTTAGATCTGGTCAACCGTGGCAAGATGAC
	TTCGTTTCAGACCAAAAAAAAAAGCAAGATGAACTAACGAAAGGTAACGCACCACTCATA
	AAACAATTGAAAGAGATGGGCCTCTTGCCTTTAGTTAATCCCTTTTTTAGACATTTGTTG
	GATCCCGAGGGTAAGGGTGTATCCCCATGGGACAGATTGGCCGTAAGGGCCGCGGTGGCG
	CACTTCATCTCTTGGGAAAGTTGGAACCACAGAACAAGAGCTGAGTATAACAGTTTGAAA
	CTGCGAAGAGATGAATTTGAGGCCGCATCTGATGAATTCAAGGACGATTTTACATTGCTA
	CGACAATATGAGGCTAAGCGACATAGTACGCTTAAGTCAATTGCCTTAGCTGATGACTCT
	AACCCGTACCGAATTGGTGTAAGGTCCTTGAGAGCCTGGAATAGGGTTAGAGAAGAATGG
	ATTGACAAAGGCGCAACCGAGGAACAAAGGGTTACCATCCTTAGTAAGCTTCAAACACAA
	TTACGGGGTAAATTCGGTGATCCAGACCTATTTAATTGGCTAGCCCAAGATAGACACGTA
	CACCTGTGGTCCCCGAGAGATTCCGTCACGCCCCTCGTAAGGATTAATGCCGTCGACAAA
	GTGCTTAGAAGACGTAAGCCTTATGCACTGATGACTTTTGCACATCCGAGATTCCATCCA
	AGATGGATTCTATACGAAGCGCCTGGTGGTTCTAACTTGCGACAATACGCTTTAGATTGT
	ACTGAAAATGCTCTGCATATTACACTTCCATTACTCGTCGACGACGCCCATGGTACATGG
	ATTGAGAAAAAAATCCGCGTACCACTCGCTCCTAGTGGACAAATACAAGATTTAACTTTA
	GAAAAACTTGAAAAGAAAAAAAACAGATTATACTATAGATCAGGATTCCAACAATTTGCT
	GGATTAGCCGGTGGTGCTGAGGTGTTGTTTCATAGGCCGTATATGGAACATGATGAGAGA
	TCAGAAGAATCTCTGTTGGAAAGGCCAGGCGCTGTGTGGTTCAAATTAACCTTAGATGTT
	GCTACCCAAGCACCACCTAACTGGTTAGATGGTAAAGGCAGAGTTAGGACACCTCCAGAA
	GTTCATCATTTCAAAACCGCTCTGTCAAATAAATCTAAACATACGAGAACCTTGCAACCA
	GGATTGAGAGTCCTTTCTGTTGATTTGGGTATGAGAACATTTGCTTCTTGTTCTGTTTTC
	GAATTGATCGAAGGTAAACCTGAAACAGGTAGAGCATTCCCTGTTGCTGACGAAAGATCA
	ATGGATAGTCCAAATAAGTTATGGGCCAAGCACGAGAGAAGCTTTAAACTAACTCTGCCT
	GGAGAAACACCGAGCAGAAAGGAGGAAGAAGAGAGAAGCATTGCTAGGGCAGAGATTTAC
	GCGCTGAAAAGAGATATTCAAAGACTGAAATCACTCCTAAGATTAGGTGAGGAAGATAAT
	GATAATAGAAGAGATGCTTTGTTAGAGCAATTCTTTAAAGGATGGGGTGAAGAGGACGTA
	GTTCCTGGTCAAGCTTTCCCTAGAAGCCTCTTTCAGGGATTAGGCGCTGCACCCTTTAGG
	TCAACACCCGAATTGTGGAGACAGCACTGTCAGACGTATTACGACAAAGCGGAAGCTTGC
	CTGGCAAAGCATATTTCCGACTGGAGGAAGAGAACTAGACCTCGTCCGACTTCGAGAGAG
	ATGTGGTATAAGACAAGATCTTACCATGGTGGCAAAAGTATTTGGATGCTAGAATACTTA
	GATGCTGTCCGCAAATTACTACTTTCATGGTCGTTAAGAGGTCGTACTTACGGAGCTATT
	AATAGACAAGACACCGCTCGTTTTGGTTCCTTAGCTTCTAGATTGTTGCATCATATCAAC
	TCTTTAAAGGAAGACCGCATCAAAACCGGTGCAGATAGTATTGTGCAGGCCGCAAGGGGC
	TATATTCCTCTCCCACATGGCAAGGGTTGGGAACAGCGTTATGAACCCTGTCAGTTGATA
	TTATTTGAAGATCTAGCTAGGTACAGATTTCGTGTAGACAGACCTCGGAGAGAGAATTCG
	CAATTGATGCAGTGGAATCATCGAGCTATAGTAGCAGAAACGACGATGCAAGCTGAACTA
	TACGGTCAAATAGTCGAAAATACCGCTGCTGGTTTCTCCTCAAGATTTCATGCTGCAACT
	GGTGCTCCTGGTGTCAGATGTCGCTTTTTGTTAGAACGAGATTTCGATAATGACCTACCA
	AAGCCGTACTTACTGAGAGAACTAAGTTGGATGTTAGGTAACACAAAGGTTGAATCAGAG
	GAAGAAAAATTGCGTCTTCTAAGCGAGAAAATTAGACCAGGTTCATTAGTCCCTTGGGAT
	GGGGGTGAACAATTCGCGACATTACACCCGAAAAGACAAACTCTTTGTGTCATTCACGCA
	GATATGAACGCTGCTCAAAACCTGCAACGCAGATTTTTCGGAAGGTGTGGGGAAGCCTTT
	CGCCTTGTGTGTCAGCCACATGGTGATGATGTTTTGAGGCTAGCGTCTACACCAGGTGCA
	AGACTTTTGGGTGCATTACAACAACTGGAAAATGGTCAGGGAGCTTTCGAATTAGTTCGT
	GATATGGGTAGCACATCACAAATGAATCGTTTCGTCATGAAGTCGTTGGGCAAAAAAAAG
	ATCAAGCCATTACAAGACAATAACGGGGATGATGAACTAGAAGACGTGCTATCTGTTTTA
	CCTGAAGAAGATGATACCGGACGAATTACTGTATTTCGGGACTCTTCGGGTATATTCTTC
	CCTTGTAACGTTTGGATCCCGGCAAAACAGTTCTGGCCTGCGGTCCGTGCTATGATTTGG
	AAGGTTATGGCATCACATTCATTGGGTTAG

SEQ	ATGACAAAGTTAAGGCATAGACAGAAGAAGTTAACTCACGATTGGGCGGGGTCTAAAAAG
ID	AGAGAAGTTCTAGGGAGCAATGGTAAATTACAGAATCCATTGCTAATGCCCGTCAAAAAA
NO:	GGTCAGGTGACAGAATTTCGAAAAGCATTTTCCGCATACGCCCGAGCAACCAAAGGGGAA
142	ATGACGGATGGCAGAAAAAATATGTTTACTCACTCATTTGAACCATTCAAGACCAAGCCT
	TCGTTACATCAGTGCGAACTGGCTGACAAAGCCTACCAGAGCTTGCATTCATATTTACCG
	GGTTCTTTGGCGCATTTTCTTTTATCTGCCCATGCACTTGGTTTTAGGATTTTTAGCAAA
	TCAGGGGAAGCCACTGCATTCCAAGCGTCCTCAAAGATTGAAGCTTACGAAAGCAAGTTA
	GCTAGCGAGCTTGCTTGTGTTGATTTGTCTATTCAGAACTTGACTATTTCAACTTTGTTC
	AACGCATTAACGACTTCCGTAAGAGGTAAAGGTGAGGAGACATCGGCAGATCCACTGATA
	GCTAGATTTTACACCTTACTTACCGGTAAACCACTAAGCAGAGACACTCAGGGCCCAGAA
	CGAGATTTAGCCGAGGTGATAAGCAGAAAAATTGCAAGTTCTTTTGGAACTTGGAAGGAG
	ATGACTGCCAATCCACTTCAATCTCTTCAATTTTTTGAAGAGGAGTTGCATGCGCTAGAT
	GCAAATGTTAGTTTGTCACCTGCCTTCGATGTTCTGATTAAGATGAACGACCTGCAGGGT
	GACTTGAAGAACAGAACGATAGTTTTTGATCCAGATGCTCCTGTGTTTGAATATAATGCT
	GAGGATCCTGCTGACATCATCATTAAACTGACAGCTAGATATGCGAAAGAAGCAGTGATT
	AAAAATCAAAATGTCGGGAATTATGTTAAGAACGCTATTACGACAACTAACGCAAACGGA
	CTAGGTTGGTTGCTGAACAAAGGCCTTTCCTTATTGCCTGTCTCCACTGATGACGAACTA
	TTGGAGTTTATTGGGGTCGAGAGATCCCATCCTAGCTGTCATGCGTTGATAGAACTTATC
	GCTCAGTTAGAAGCACCTGAACTGTTCGAAAAAAATGTTTTTTCTGATACTCGTTCCGAG
	GTTCAAGGTATGATAGATTCAGCTGTAAGCAATCATATCGCCAGGCTGTCAAGCTCTCGT
	AATTCATTGAGCATGGACTCAGAGGAACTTGAGAGATTGATAAAATCTTTTCAAATTCAT
	ACACCACATTGTTCATTATTTATAGGGGCTCAATCCTTATCTCAACAATTGGAAAGCCTA
	CCCGAAGCATTGCAGTCAGGAGTGAACAGTGCTGATATTCTGCTCGGCTCAACCCAATAC
	ATGTTGACAAATTCTTTGGTCGAGGAGTCAATCGCTACGTATCAGAGAACCTTAAATAGA
	ATTAACTACCTGTCCGGCGTTGCAGGACAGATTAACGGTGCTATTAAGAGGAAAGCTATT
	GATGGTGAGAAGATACATTTACCCGCTGCTTGGTCAGAGTTAATTTCTTTACCCTTTATT
	GGGCAACCAGTGATTGATGTTGAATCAGATTTAGCCCACTTAAAGAACCAATACCAGACA
	TTGTCTAACGAATTTGATACGCTGATTTCCGCACTGCAAAAGAATTTCGACTTAAATTTT
	AATAAAGCCTTGCTTAATCGAACACAACATTTCGAGGCTATGTGTAGATCAACAAAAAAG
	AATGCCCTTTCTAAGCCTGAGATCGTTAGTTATAGAGATTTGCTAGCCAGGTTGACTTCT
	TGTCTTTATAGGGGCTCTCTAGTCTTGAGGAGGGCGGGTATAGAAGTACTGAAAAAGCAC
	AAGATATTTGAGTCCAACTCTGAATTAAGAGAGCACGTTCATGAAAGAAAACACTTCGTA
	TTTGTTTCTCCGCTCGATAGAAAAGCCAAGAAGCTCCTACGTTTGACTGACTCTAGGCCT
	GATTTATTGCACGTAATTGATGAAATACTACAACATGATAATTTAGAGAACAAGGATAGA
	GAATCTTTGTGGTTAGTTCGATCTGGTTATTTACTGGCCGGCCTACCAGACCAACTCTCC
	TCTTCCTTTATAAATCTTCCAATCATTACTCAAAAAGGCGATCGTCGCTTGATAGATCTC
	ATTCAATACGACCAAATTAATAGAGATGCTTTTGTGATGTTGGTAACTTCCGCTTTTAAG
	TCGAACTTAAGTGGGCTGCAGTACAGAGCAAACAAACAATCTTTTGTGGTTACGCGCACT
	TTGTCACCATATTTGGGATCTAAATTGGTTTATGTGCCCAAAGATAAAGATTGGCTGGTC
	CCTTCCCAAATGTTCGAGGGGAGATTTGCGGACATTTTGCAATCCGATTATATGGTGTGG
	AAGGACGCTGGAAGATTGTGTGTTATTGACACAGCTAAGCATTTGTCTAACATTAAAAAA
	TCTGTATTCTCAAGTGAAGAAGTCCTCGCGTTTTTAAGAGAATTGCCACACCGTACGTTT
	ATCCAAACTGAGGTCAGGGGTTTAGGGGTGAATGTGGACGGTATTGCATTTAATAACGGG
	GATATACCCTCTCTGAAGACGTTTAGCAATTGCGTGCAAGTCAAAGTGAGTCGGACAAAC
	ACTAGTCTGGTCCAAACATTAAATAGATGGTTTGAAGGCGGTAAGGTCTCGCCGCCTAGC
	ATCCAATTTGAGAGAGCATATTACAAAAAAGATGATCAAATCCACGAGGACGCTGCAAAA
	AGGAAGATAAGGTTTCAAATGCCAGCTACAGAGTTGGTACACGCGTCAGACGACGCAGGA
	TGGACCCCCTCCTATTTACTTGGTATCGATCCCGGTGAATATGGTATGGGTTTGTCATTG
	GTCTCAATAAATAATGGCGAAGTTTTAGATAGCGGATTTATACACATAAATTCATTGATA
	AATTTCGCTTCTAAGAAATCAAATCATCAAACCAAAGTTGTTCCGAGGCAGCAATACAAG
	TCACCATACGCCAACTATCTAGAACAATCTAAAGATTCTGCAGCAGGAGACATAGCTCAT
	ATTTTGGATAGACTTATCTACAAGTTGAACGCCCTACCCGTTTTCGAAGCTCTATCTGGC
	AATAGTCAAAGCGCAGCGGATCAGGTTTGGACAAAAGTCCTCAGCTTCTACACCTGGGGA
	GATAATGATGCACAAAATTCAATTCGTAAGCAACATTGGTTCGGTGCTTCACACTGGGAC
	ATTAAAGGCATGTTGAGGCAACCGCCAACAGAAAAAAAGCCCAAACCATACATTGCCTTT
	CCCGGTTCACAAGTTTCTTCTTATGGTAATTCTCAAAGGTGTTCATGTTGTGGACGTAAC
	CCAATTGAACAATTGCGCGAAATGGCGAAGGACACATCCATTAAGGAGTTGAAGATTAGA
	AATTCAGAAATTCAATTGTTCGACGGTACTATAAAGTTATTTAATCCAGACCCGTCAACG
	GTCATAGAAAGAAGAAGACATAATTTAGGGCCATCAAGAATTCCTGTAGCTGATAGAACT
	TTCAAAAATATAAGTCCAAGCTCACTAGAATTCAAAGAACTAATAACGATTGTGTCACGG
	TCTATACGTCATTCCCCAGAATTTATTGCTAAAAAAAGAGGTATAGGTAGTGAGTACTTT
	TGTGCTTATAGTGATTGTAATTCCTCCTTAAATTCAGAAGCAAATGCGGCTGCGAACGTT
	GCCCAAAAGTTCCAAAAGCAATTGTTTTTCGAATTATAG

SEQ	ATGAAAAGAATCTTGAACTCTTTAAAGGTTGCCGCCCTGCGTTTGTTATTTAGAGGTAAA
ID	GGATCTGAACTTGTCAAGACTGTTAAATACCCTTTGGTCTCGCCGGTTCAGGGTGCAGTT
NO:	GAGGAGTTAGCTGAGGCGATCCGCCATGATAACCTACATCTGTTTGGTCAAAAAGAAATT
143	GTTGACCTTATGGAAAAGGATGAAGGTACGCAAGTTTACTCAGTGGTTGATTTCTGGTTA
	GATACCCTTCGTTTGGGGATGTTTTTCAGTCCATCAGCAAACGCATTAAAAATCACGCTG
	GGTAAGTTTAATTCTGATCAGGTTAGCCCTTTTAGGAAAGTGTTAGAGCAGTCTCCATTC
	TTCTTGGCTGGTAGGCTGAAGGTTGAACCGGCAGAACGTATATTATCTGTCGAGATCCGT
	AAGATTGGGAAGAGGGAAAACAGAGTTGAGAACTATGCTGCTGACGTAGAAACGTGTTTT
	ATAGGCCAATTAAGTTCAGATGAGAAACAGTCAATACAAAAATTAGCTAATGATATCTGG
	GATAGTAAAGATCATGAAGAGCAAAGAATGTTAAAGGCAGATTTCTTCGCTATCCCTTTG
	ATTAAGGATCCAAAGGCTGTGACCGAAGAGGATCCTGAAAATGAAACTGCTGGTAAACAA
	AAACCCTTGGAGTTGTGTGTCTGCCTTGTCCCAGAACTTTACACAAGAGGATTCGGGTCA
	ATAGCCGATTTTTTGGTTCAACGCTTAACTCTTTTAAGGGATAAAATGTCTACAGATACT
	GCAGAAGATTGTTTAGAATATGTCGGGATTGAGGAGGAAAAAGGTAACGGCATGAACTCA
	TTGTTGGGAACGTTCTTAAAGAATTTGCAAGGCGATGGATTTGAGCAGATTTTCCAATTT
	ATGTTAGGGAGCTATGTCGGTTGGCAAGGGAAGGAAGATGTTTTAAGAGAGAGATTAGAC
	TTATTGGCTGAAAAAGTGAAGAGGTTACCGAAACCAAAATTTGCTGGCGAATGGTCTGGT
	CATAGGATGTTCTTGCATGGCCAATTGAAGTCTTGGTCTTCAAATTTTTTTAGACTATTT
	AACGAGACAAGGGAACTTCTAGAGTCTATTAAGTCAGATATACAGCATGCCACAATGCTA
	ATATCATATGTAGAAGAAAAAGGTGGTTATCATCCTCAATTACTTAGTCAATATAGAAAA
	CTTATGGAACAACTACCAGCTTTGCGTACCAAGGTATTGGACCCTGAGATTGAAATGACA
	CATATGTCCGAAGCAGTTCGCTCTTATATAATGATACATAAATCTGTTGCGGGTTTTTTA
	CCGGATTTATTAGAATCATTAGATAGAGACAAGGATCGTGAGTTTCTGCTTAGTATTTTT
	CCAAGAATCCCAAAAATTGATAAAAAAACCAAGGAAATTGTAGCTTGGGAACTGCCGGGA
	GAACCAGAAGAAGGTTATTTATTTACTGCTAATAACTTGTTCAGAAACTTCTTAGAGAAT
	CCGAAACATGTCCCGAGATTTATGGCCGAAAGGATCCCAGAAGATTGGACTCGATTACGC
	TCTGCTCCTGTCTGGTTCGATGGAATGGTAAAACAATGGCAAAAAGTCGTTAACCAGTTA
	GTAGAATCACCAGGTGCTTTATATCAATTTAACGAATCCTTCTTGAGACAAAGGTTACAG
	GCCATGTTAACTGTGTATAAGAGGGACTTACAAACTGAAAAATTTCTTAAACTTTTGGCG
	GATGTTTGTAGGCCTCTTGTAGATTTTTTTGGTTTGGGTGGAAATGATATTATTTTTAAG
	AGCTGTCAAGACCCAAGAAAACAATGGCAAACCGTTATTCCTCTCTCTGTTCCGGCAGAT
	GTCTATACTGCTTGCGAAGGTTTGGCGATTAGACTAAGGGAGACATTAGGATTCGAATGG
	AAGAATTTGAAAGGTCACGAGAGAGAAGATTTCTTAAGATTGCACCAGTTATTGGGCAAT
	TTACTTTTCTGGATTCGTGATGCTAAATTGGTAGTAAAATTAGAGGATTGGATGAACAAC
	CCATGTGTTCAGGAATATGTAGAAGCCCGGAAAGCTATCGATCTTCCACTAGAAATATTC
	GGTTTTGAAGTGCCTATCTTCCTGAATGGCTATCTATTTTCGGAGTTGAGACAATTAGAA
	CTTTTGCTTAGGAGAAAAAGTGTGATGACTAGCTACAGTGTAAAGACTACTGGATCTCCT
	AATAGGCTATTTCAGCTAGTTTATTTACCTCTAAACCCTAGTGACCCCGAAAAGAAGAAC
	TCAAATAACTTTCAAGAACGTTTGGATACCCCAACTGGTTTGTCCCGTCGTTTCCTAGAC
	CTAACCCTTGATGCATTCGCAGGTAAGTTACTTACCGATCCAGTTACACAAGAATTGAAG
	ACAATGGCAGGTTTTTACGATCATCTTTTTGGATTCAAATTGCCATGTAAACTCGCCGCC
	ATGTCGAATCATCCAGGTTCTTCTTCAAAGATGGTTGTGTTAGCGAAACCCAAAAAAGGT
	GTTGCTTCTAATATAGGGTTTGAACCGATCCCAGATCCCGCTCATCCCGTATTTAGGGTT
	AGATCCAGTTGGCCAGAGTTGAAGTACCTCGAGGGGCTATTGTATTTGCCAGAAGACACA
	CCTTTGACCATCGAATTAGCAGAGACCTCCGTATCGTGCCAAAGTGTCTCGTCAGTTGCA
	TTCGATTTGAAAAACTTGACAACGATCTTAGGTCGTGTGGGAGAATTTAGGGTCACAGCT
	GATCAACCCTTTAAACTAACGCCTATAATCCCGGAGAAAGAAGAATCTTTTATTGGTAAA
	ACTTATTTGGGTCTCGACGCGGGTGAAAGGAGCGGCGTCGGTTTCGCTATTGTTACAGTG
	GACGGAGATGGGTACGAAGTGCAAAGATTGGGGGTCCACGAGGATACACAGCTTATGGCC
	TTGCAGCAAGTTGCTAGTAAATCCTTAAAAGAGCCAGTATTTCAGCCTCTAAGAAAAGGC
	ACCTTTAGACAACAAGAAAGAATACGGAAATCCTTACGTGGTTGCTACTGGAATTTTTAT
	CATGCCTTGATGATAAAATATAGGGCCAAAGTAGTACATGAGGAATCTGTCGGAAGTAGT
	GGTCTTGTGGGTCAATGGTTGAGGGCTTTTCAGAAGGATTTGAAGAAAGCCGATGTTCTC
	CCCAAGAAGGGCGGTAAAAACGGTGTAGATAAGAAGAAGAGAGAGTCCTCAGCTCAAGAC
	ACTCTTTGGGGTGGTGCTTTCTCTAAAAAGGAGGAGCAACAGATTGCGTTTGAGGTGCAA
	GCTGCAGGTTCTTCGCAATTTTGTTTGAAGTGCGGATGGTGGTTCCAACTAGGCATGCGT
	GAAGTAAACAGGGTACAAGAATCGGGCGTCGTGTTAGATTGGAATAGAAGCATAGTTACC
	TTTTTAATAGAATCATCCGGCGAAAAAGTTTATGGTTTCTCCCCACAGCAATTAGAGAAG
	GGTTTCAGACCAGACATCGAAACTTTTAAAAAGATGGTAAGAGACTTTATGAGACCTCCT
	ATGTTTGATAGAAAAGGCAGACCGGCCGCAGCTTACGAGAGATTTGTTTTAGGAAGGAGA
	CATCGAAGGTACAGGTTTGATAAAGTATTTGAGGAAAGATTTGGGAGGTCTGCTCTTTTC
	ATTTGTCCTAGAGTAGGTTGTGGAAATTTTGACCACAGCTCCGAACAGTCCGCGGTTGTT
	TTGGCCTTGATCGGATATATTGCCGATAAGGAGGGAATGTCAGGTAAGAAGTTGGTTTAT
	GTACGGCTGGCCGAACTTATGGCCGAATGGAAACTAAAAAAATTAGAAAGATCCAGAGTT
	GAAGAACAATCATCCGCTCAATAA

SEQ	ATGGCAGAAAGCAAACAAATGCAGTGTAGGAAATGTGGAGCTAGTATGAAGTACGAAGTC
ID	ATCGGTTTGGGTAAAAAGTCATGTAGATACATGTGTCCCGATTGTGGCAACCATACCTCG
NO:	GCAAGAAAGATACAAAACAAAAAAAAAAGAGATAAAAAATATGGGTCAGCCAGTAAAGCC
144	CAATCTCAAAGAATTGCTGTAGCAGGTGCTCTTTACCCTGACAAAAAAGTACAAACTATC
	AAAACCTATAAATATCCAGCAGACTTGAATGGTGAGGTGCATGATAGCGGTGTTGCCGAG
	AAAATCGCACAAGCAATACAAGAGGACGAGATTGGACTTTTGGGACCAAGCTCAGAATAT
	GCATGCTGGATTGCATCTCAAAAACAGTCTGAGCCTTACAGTGTAGTCGATTTCTGGTTT
	GATGCAGTGTGCGCAGGGGGAGTCTTCGCCTACTCTGGCGCTAGATTATTGAGTACAGTT
	TTACAGTTATCCGGTGAGGAATCGGTGCTTAGAGCTGCCTTAGCCTCGTCTCCATTCGTT
	GACGATATAAACTTAGCGCAAGCCGAAAAGTTTTTGGCGGTTAGCAGGCGTACAGGTCAA
	GATAAGTTAGGTAAGAGAATTGGGGAGTGCTTTGCAGAAGGAAGATTGGAAGCTTTAGGG
	ATAAAAGATAGAATGAGGGAATTTGTTCAAGCTATCGATGTTGCACAGACCGCCGGACAA
	CGTTTCGCTGCCAAATTGAAGATATTCGGTATAAGTCAGATGCCAGAAGCTAAGCAATGG
	AATAACGATTCCGGACTGACTGTCTGTATACTACCTGATTATTATGTTCCCGAAGAGAAT
	CGCGCGGACCAACTTGTAGTGTTGTTAAGAAGACTTCGCGAGATTGCATATTGCATGGGT
	ATTGAAGATGAAGCGGGTTTCGAACATCTTGGAATAGATCCTGGTGCTCTTTCGAATTTT
	TCAAACGGTAACCCTAAGAGAGGATTTCTAGGGAGGCTGTTAAATAACGATATTATTGCG
	TTGGCAAACAATATGAGTGCGATGACTCCATATTGGGAAGGGCGTAAGGGTGAACTCATA
	GAAAGGCTTGCGTGGTTAAAGCACAGGGCAGAAGGGCTGTATCTTAAAGAACCTCATTTC
	GGTAACTCCTGGGCCGATCATAGGTCACGAATTTTCTCAAGGATCGCAGGCTGGTTATCT
	GGTTGCGCTGGCAAGTTGAAAATTGCGAAAGACCAAATTTCTGGAGTACGTACAGATCTA
	TTTCTGCTAAAAAGACTGCTGGACGCAGTTCCGCAATCGGCGCCATCCCCCGATTTTATT
	GCGTCAATTTCGGCACTTGACAGGTTTTTAGAAGCTGCAGAATCGAGCCAGGACCCTGCT
	GAACAAGTGAGGGCTCTCTACGCTTTTCACTTGAACGCACCTGCAGTCCGAAGTATAGCC
	AATAAAGCAGTGCAAAGGTCCGACAGCCAAGAATGGCTGATAAAAGAACTAGACGCTGTT
	GACCATTTAGAATTTAACAAAGCGTTCCCATTTTTCTCTGACACAGGAAAAAAAAAAAAA
	AAAGGTGCTAATAGCAACGGTGCTCCATCGGAAGAAGAGTACACTGAAACGGAATCAATA
	CAACAACCTGAGGACGCGGAACAGGAAGTAAACGGACAAGAAGGGAACGGAGCGTCTAAA
	AATCAAAAGAAATTTCAAAGAATACCTAGATTCTTCGGTGAAGGCTCCAGATCTGAATAC
	AGAATTTTAACGGAAGCTCCACAGTATTTCGATATGTTTTGTAATAACATGAGGGCTATA
	TTTATGCAGTTAGAAAGTCAACCCCGTAAAGCTCCCAGAGATTTTAAATGTTTCCTACAA
	AATCGATTACAAAAATTATACAAACAGACTTTCTTGAATGCACGAAGCAACAAGTGTCGC
	GCTCTGCTTGAGTCAGTTTTAATCTCTTGGGGAGAATTTTATACATACGGTGCCAACGAA
	AAGAAATTTAGATTAAGACATGAAGCTTCAGAACGCAGCAGTGACCCAGATTACGTAGTT
	CAGCAAGCCTTGGAAATCGCGCGTCGTCTATTCCTTTTTGGCTTCGAATGGAGAGATTGC
	TCCGCTGGTGAAAGAGTGGATTTGGTTGAAATTCACAAAAAGGCTATCAGTTTTTTGTTG
	GCTATTACTCAAGCTGAGGTCTCTGTTGGTTCATACAATTGGCTTGGCAACTCAACAGTA
	TCGAGATATTTATCCGTTGCGGGAACTGATACCTTATACGGTACCCAATTGGAAGAATTC
	CTGAACGCTACAGTGTTGAGTCAAATGCGTGGTCTGGCCATTAGATTGAGTTCTCAAGAA
	CTTAAGGACGGTTTTGATGTGCAGCTCGAGTCTTCCTGCCAGGACAATCTGCAACACCTA
	TTGGTGTATAGGGCTTCGAGAGATTTGGCGGCTTGCAAGCGCGCTACTTGTCCAGCCGAA
	CTCGATCCTAAGATTTTAGTTTTACCGGTAGGTGCATTCATCGCTTCCGTAATGAAAATG
	ATAGAAAGAGGTGACGAACCTTTAGCTGGTGCTTATTTACGGCATAGGCCACACTCTTTC
	GGATGGCAAATTAGGGTCCGCGGTGTTGCTGAGGTAGGGATGGATCAGGGTACAGCATTG
	GCCTTTCAAAAGCCAACAGAGTCAGAACCTTTTAAAATTAAGCCCTTCTCTGCACAGTAT
	GGACCAGTTCTGTGGTTGAACAGTAGTAGTTATTCTCAATCACAATATTTGGACGGTTTT
	CTATCTCAACCAAAAAATTGGAGTATGAGGGTGTTGCCTCAGGCGGGTTCAGTTCGCGTC
	GAACAACGAGTTGCTTTGATATGGAACTTACAAGCAGGCAAGATGAGACTAGAACGCTCC
	GGTGCGAGGGCCTTTTTCATGCCTGTACCGTTTTCATTTAGGCCATCCGGCAGTGGGGAC
	GAAGCAGTTTTGGCGCCCAACCGGTACTTGGGTCTGTTCCCTCATTCCGGAGGTATAGAA
	TACGCTGTAGTGGATGTCCTGGATTCTGCTGGATTTAAAATTCTTGAAAGAGGCACTATT
	GCTGTCAATGGTTTCTCTCAGAAAAGGGGAGAGCGCCAAGAAGAAGCCCATCGTGAAAAA
	CAAAGAAGGGGGATAAGTGATATAGGGCGAAAGAAGCCTGTGCAGGCAGAAGTCGATGCG
	GCGAACGAATTGCATAGAAAGTACACTGATGTTGCCACAAGATTAGGTTGTAGAATCGTC
	GTTCAATGGGCACCACAACCTAAACCAGGGACAGCACCGACAGCGCAAACTGTTTACGCG
	AGGGCTGTTAGGACAGAAGCTCCGAGGAGCGGCAACCAAGAAGATCATGCAAGAATGAAA
	AGTTCTTGGGGTTACACCTGGGGTACGTATTGGGAGAAACGAAAACCAGAAGATATTTTA
	GGGATTTCTACACAGGTGTATTGGACAGGAGGTATAGGCGAATCCTGTCCTGCTGTAGCA
	GTCGCTTTATTAGGTCATATTAGAGCAACTTCAACACAAACGGAGTGGGAAAAGGAAGAA
	GTTGTCTTTGGAAGACTGAAGAAGTTCTTTCCGAGTTAA

SEQ	ATGGAGAAGAGAATTAATAAGATACGGAAAAAATTATCTGCGGATAATGCAACAAAGCCA
ID	GTCTCTCGTTCAGGCCCCATGAAAACCCTGCTTGTAAGAGTAATGACGGATGATTTAAAA
NO:	AAGAGGTTGGAAAAGCGTAGAAAAAAACCAGAAGTGATGCCGCAAGTGATCTCAAATAAC
145	GCAGCTAATAATCTAAGGATGCTACTTGATGATTATACAAAAATGAAAGAAGCAATCCTG
	CAAGTTTACTGGCAGGAATTCAAGGATGACCATGTTGGACTAATGTGCAAATTCGCACAA
	CCAGCGTCTAAGAAAATTGACCAAAATAAATTGAAACCCGAAATGGACGAAAAAGGGAAT
	TTAACAACTGCCGGGTTTGCCTGCTCGCAATGTGGGCAACCATTATTTGTTTATAAATTA
	GAGCAGGTTTCGGAAAAAGGAAAGGCTTACACAAATTACTTCGGCAGATGTAATGTTGCC
	GAACACGAAAAACTCATATTGTTAGCTCAGTTGAAGCCTGAGAAAGACTCTGATGAGGCC
	GTTACTTACTCGTTGGGGAAGTTTGGTCAAAGAGCTCTCGATTTTTATTCTATTCATGTG
	ACAAAGGAGTCCACACATCCCGTCAAGCCCTTGGCACAAATTGCGGGTAATAGATACGCT
	TCGGGTCCAGTTGGGAAGGCCCTTTCTGATGCATGTATGGGCACAATTGCTAGCTTTCTT
	AGTAAATACCAGGATATCATAATAGAGCATCAAAAAGTTGTAAAGGGTAACCAAAAGAGA
	TTAGAATCGCTGCGTGAGTTGGCGGGTAAAGAAAACTTGGAATATCCATCTGTCACTCTG
	CCTCCTCAACCTCATACTAAGGAAGGTGTAGATGCGTACAATGAAGTTATCGCTAGAGTC
	CGTATGTGGGTGAATTTAAATTTGTGGCAAAAATTGAAGTTATCGCGTGATGATGCAAAA
	CCTCTTCTTAGACTAAAGGGCTTTCCTAGCTTCCCTGTAGTGGAAAGACGCGAAAATGAA
	GTCGATTGGTGGAATACAATTAACGAAGTCAAAAAACTGATCGATGCAAAGCGAGATATG
	GGTCGAGTTTTTTGGTCTGGTGTTACAGCTGAAAAAAGGAATACGATCTTAGAAGGTTAC
	AACTACTTGCCAAATGAGAACGATCATAAAAAAAGAGAAGGCAGTTTAGAAAATCCAAAA
	AAGCCAGCTAAGAGACAATTTGGTGATTTGCTACTTTACCTAGAAAAAAAGTACGCCGGA
	GATTGGGGGAAAGTCTTTGACGAAGCTTGGGAGAGAATAGATAAAAAAATAGCAGGATTG
	ACGTCACACATTGAAAGAGAAGAGGCGAGAAATGCAGAAGATGCTCAGTCCAAAGCTGTC
	CTCACCGACTGGTTGAGAGCCAAAGCGTCCTTTGTTCTCGAACGCCTAAAAGAAATGGAT
	GAGAAGGAATTTTATGCCTGCGAAATCCAGCTACAAAAATGGTACGGAGACTTGAGAGGT
	AACCCCTTTGCCGTGGAAGCAGAGAACCGTGTTGTAGATATCTCCGGTTTCTCAATCGGT
	AGCGATGGACACTCCATTCAGTATCGCAACTTGTTGGCCTGGAAATATTTGGAAAACGGT
	AAGAGGGAATTCTATTTACTTATGAATTATGGCAAGAAAGGTAGAATCAGGTTTACTGAC
	GGAACAGACATTAAAAAGAGTGGTAAGTGGCAAGGCCTTTTGTACGGTGGTGGCAAGGCC
	AAAGTAATAGACTTAACATTTGACCCCGACGACGAACAACTGATAATACTGCCTTTAGCT
	TTTGGTACTCGACAGGGGCGAGAGTTCATTTGGAATGATCTTTTGTCACTCGAGACTGGT
	TTGATAAAACTTGCAAATGGAAGAGTCATCGAGAAGACAATTTACAACAAAAAGATAGGT
	CGCGATGAGCCTGCACTATTTGTGGCCTTGACCTTTGAGAGAAGGGAAGTTGTCGACCCA
	TCCAATATTAAACCAGTCAACCTAATCGGTGTAGATAGAGGTGAAAACATCCCAGCTGTT
	ATCGCTCTGACAGACCCTGAAGGTTGCCCTTTGCCAGAATTTAAAGATTCGTCTGGTGGA
	CCAACAGATATATTACGTATTGGGGAAGGCTATAAAGAGAAACAACGTGCTATTCAGGCT
	GCAAAAGAAGTTGAACAGAGGAGAGCTGGAGGTTACAGTAGAAAATTCGCCAGTAAAAGT
	AGAAACTTAGCAGATGACATGGTTAGAAACTCTGCCCGGGATTTGTTCTATCATGCGGTT
	ACTCACGATGCAGTCTTAGTCTTTGAAAATCTATCGCGCGGTTTTGGTAGGCAAGGCAAG
	AGGACTTTTATGACAGAGAGACAATATACAAAAATGGAAGATTGGTTAACCGCGAAGCTC
	GCATATGAAGGTCTTACTTCGAAAACGTACCTCAGCAAAACGCTGGCTCAATATACTTCT
	AAAACTTGTTCAAATTGTGGTTTTACTATTACCACGGCAGACTACGACGGGATGTTGGTG
	AGATTGAAGAAGACGAGCGATGGTTGGGCAACAACATTGAATAATAAGGAATTAAAAGCA
	GAAGGACAGATTACGTATTACAATCGTTATAAACGCCAAACGGTTGAGAAAGAGTTGTCA
	GCCGAGTTGGATAGACTAAGTGAAGAGAGCGGTAACAATGATATCTCAAAGTGGACTAAA
	GGGAGGCGGGATGAAGCCCTCTTTTTACTAAAGAAGAGATTCTCACATAGACCTGTGCAA
	GAACAATTCGTTTGTTTAGATTGTGGCCATGAGGTTCATGCAGACGAACAGGCTGCGTTA
	AATATTGCGAGAAGCTGGCTATTTCTAAATTCTAATTCAACAGAGTTCAAGAGCTATAAA
	TCCGGAAAACAACCTTTCGTAGGCGCGTGGCAAGCCTTCTATAAAAGGAGATTAAAAGAG
	GTTTGGAAACCAAATGCA

SEQ	ATGAAAAGAATTAACAAAATTAGAAGGAGGCTGGTCAAAGATTCTAATACCAAGAAAGCT
ID	GGTAAGACTGGTCCGATGAAAACCCTATTAGTCAGAGTTATGACCCCAGATTTGAGAGAA
NO:	AGATTGGAGAACCTCAGGAAAAAGCCCGAAAACATCCCACAACCCATTAGTAACACATCA
146	AGAGCTAATTTAAACAAGTTATTAACTGACTACACTGAAATGAAAAAAGCAATATTGCAT
	GTTTACTGGGAAGAGTTCCAGAAAGATCCTGTTGGGTTGATGTCTAGAGTTGCTCAACCG
	GCCCCAAAGAATATAGATCAAAGGAAACTTATTCCTGTGAAGGACGGCAATGAAAGATTA
	ACCAGCTCCGGTTTCGCTTGCTCCCAGTGCTGCCAACCCCTGTATGTATACAAACTGGAA
	CAAGTAAATGATAAAGGTAAGCCACATACTAACTACTTTGGTAGGTGTAATGTATCCGAG
	CATGAAAGATTGATCTTGTTAAGTCCCCATAAACCAGAAGCTAATGATGAGTTAGTAACT
	TATAGTTTAGGTAAGTTCGGACAACGAGCTTTAGATTTCTATAGCATCCATGTTACAAGA
	GAAAGCAATCACCCCGTCAAACCACTGGAACAAATCGGTGGTAATAGTTGTGCGTCAGGT
	CCAGTAGGCAAAGCTTTATCAGACGCTTGCATGGGTGCCGTGGCTAGTTTTTTGACGAAA
	TACCAAGATATTATACTGGAACATCAAAAGGTAATTAAAAAGAATGAAAAGAGACTCGCT
	AACTTAAAAGATATTGCAAGTGCCAATGGTTTAGCTTTTCCTAAAATTACCTTGCCACCT
	CAGCCACATACAAAGGAGGGAATTGAAGCTTACAATAATGTAGTAGCCCAAATAGTTATT
	TGGGTGAACCTTAACCTATGGCAAAAGTTAAAAATTGGTAGAGACGAAGCCAAACCCCTG
	CAGAGGCTGAAGGGTTTTCCCTCCTTCCCCTTAGTAGAGAGACAAGCTAATGAAGTGGAC
	TGGTGGGATATGGTGTGCAATGTTAAAAAATTGATTAATGAGAAGAAAGAGGATGGTAAA
	GTGTTTTGGCAGAATCTTGCTGGCTACAAGAGACAGGAAGCTTTACTGCCTTATTTATCT
	TCTGAGGAAGATAGGAAAAAAGGTAAAAAATTTGCTAGATATCAATTCGGAGACCTACTT
	CTGCATTTAGAAAAAAAACATGGCGAAGATTGGGGTAAAGTTTATGATGAAGCCTGGGAA
	AGAATTGATAAGAAGGTAGAAGGTCTCTCCAAACATATTAAATTAGAGGAAGAACGTAGG
	TCCGAAGACGCTCAATCAAAGGCAGCATTAACTGATTGGTTGAGAGCAAAAGCCTCTTTC
	GTTATTGAAGGATTAAAAGAAGCCGACAAAGATGAATTTTGTAGATGTGAGTTAAAGTTG
	CAAAAGTGGTATGGAGACCTCCGTGGTAAACCTTTTGCTATTGAGGCTGAAAATTCTATA
	CTCGATATCTCTGGATTTTCAAAACAATATAACTGCGCATTTATATGGCAGAAAGATGGT
	GTTAAAAAGCTAAATCTATACTTAATTATCAATTACTTTAAAGGTGGTAAATTGCGTTTT
	AAGAAGATAAAGCCTGAAGCCTTTGAGGCAAACCGTTTTTACACTGTTATCAATAAAAAA
	TCTGGGGAAATCGTACCAATGGAAGTTAATTTCAATTTCGATGATCCTAATCTTATTATT
	TTACCTCTTGCTTTCGGCAAAAGGCAAGGTAGGGAGTTTATTTGGAATGATTTATTGTCG
	CTGGAAACGGGGTCTCTCAAACTCGCAAACGGTAGGGTGATAGAAAAAACATTATACAAC
	AGGAGAACTCGGCAGGATGAGCCAGCTCTTTTTGTGGCTCTGACATTCGAGAGAAGGGAA
	GTTTTAGATTCATCTAACATCAAACCAATGAATTTAATAGGTATTGACCGGGGTGAAAAT
	ATACCTGCAGTTATTGCTTTAACTGATCCTGAGGGATGTCCTCTTAGCAGATTCAAGGAC
	TCGTTGGGTAACCCTACTCACATCTTAAGGATTGGAGAAAGTTACAAGGAGAAACAAAGG
	ACAATACAAGCTGCTAAAGAAGTAGAACAAAGGAGGGCGGGTGGATATAGTCGGAAATAT
	GCCAGCAAGGCCAAGAATTTAGCTGACGACATGGTTAGGAATACAGCTAGAGACCTTTTA
	TACTATGCCGTCACCCAGGATGCCATGTTGATATTTGAAAATTTAAGTAGAGGCTTCGGT
	AGACAAGGTAAGCGCACCTTCATGGCAGAGAGACAATATACTAGAATGGAAGATTGGTTG
	ACTGCCAAATTGGCATACGAAGGTCTACCTAGTAAGACGTACTTATCTAAAACACTAGCG
	CAGTATACTTCCAAGACATGCAGTAATTGTGGTTTCACAATCACTTCTGCCGATTACGAT
	CGCGTCTTGGAAAAACTAAAAAAAACAGCGACAGGTTGGATGACTACTATTAATGGGAAA
	GAATTGAAGGTCGAAGGACAAATAACTTACTATAATAGATATAAACGGCAAAACGTTGTA
	AAAGACCTGTCAGTCGAACTCGATCGACTTAGTGAAGAATCTGTTAATAATGATATTAGT
	TCGTGGACAAAAGGTAGATCCGGTGAAGCTTTGAGCCTCCTGAAAAAACGTTTTAGCCAT
	AGGCCTGTCCAAGAAAAGTTTGTATGTTTAAACTGTGGTTTTGAGACCCATGCAGACGAG
	CAGGCCGCTCTTAATATTGCTAGATCATGGTTATTTTTAAGATCTCAGGAATACAAGAAG
	TACCAGACTAACAAGACAACAGGCAACACAGATAAGCGAGCATTCGTTGAGACTTGGCAA
	TCTTTTTATAGAAAGAAATTGAAGGAAGTCTGGAAACCA

SEQ	ATGGGAAAAATGTATTATCTAGGCCTGGACATAGGGACCAATTCAGTAGGCTACGCTGTC
ID	ACTGACCCCTCCTACCATTTGCTGAAGTTCAAGGGGGAACCCATGTGGGGAGCACACGTG
NO:	TTTGCGGCCGGCAACCAGAGCGCAGAGCGGAGAAGCTTCCGCACCTCCAGGAGAAGGCTG
147	GATCGCAGGCAGCAGCGTGTGAAGCTGGTCCAAGAGATATTTGCCCCAGTGATTTCCCCC
	ATCGATCCGCGCTTCTTTATTAGGCTCCACGAGTCCGCTCTCTGGCGCGACGACGTGGCC
	GAAACTGATAAACATATTTTCTTTAATGACCCAACATACACTGACAAGGAGTACTATTCA
	GATTACCCAACAATTCACCATTTGATCGTGGACCTTATGGAAAGTTCGGAGAAGCATGAT
	CCTCGACTTGTCTATTTGGCCGTGGCGTGGCTCGTGGCACATAGGGGCCACTTCTTGAAC
	GAGGTGGACAAGGATAACATCGGGGATGTGTTATCTTTCGACGCTTTCTATCCTGAATTC
	CTTGCTTTTCTGTCTGACAATGGCGTCAGCCCGTGGGTCTGCGAATCCAAGGCCCTCCAG
	GCTACGCTATTGTCAAGAAATAGCGTGAACGACAAGTACAAGGCTCTTAAGTCTTTGATT
	TTTGGAAGCCAGAAGCCCGAGGACAACTTTGATGCAAATATCTCGGAGGACGGGCTGATT
	CAGCTCCTCGCTGGGAAAAAGGTCAAGGTCAATAAGCTGTTTCCACAGGAGTCAAATGAC
	GCGAGCTTCACCCTTAACGACAAAGAGGATGCCATTGAAGAGATCCTGGGGACACTCACC
	CCAGACGAGTGCGAGTGGATAGCCCATATTAGGCGCCTCTTTGATTGGGCCATAATGAAA
	CATGCGCTTAAGGACGGGCGCACGATATCCGAAAGCAAGGTCAAATTGTACGAGCAGCAC
	CACCATGATCTGACCCAGCTAAAATATTTTGTAAAAACATATCTGGCCAAGGAGTACGAT
	GATATCTTCCGCAACGTGGATAGTGAGACCACCAAAAACTACGTCGCGTACTCATACCAC
	GTGAAAGAAGTTAAGGGCACGCTGCCTAAGAACAAGGCAACACAAGAGGAGTTCTGCAAG
	TACGTTCTCGGGAAAGTTAAAAATATAGAGTGCAGCGAGGCCGACAAAGTGGATTTTGAC
	GAGATGATTCAACGCCTGACCGACAATTCGTTTATGCCTAAACAGGTGAGTGGAGAGAAT
	CGCGTGATTCCATATCAGCTCTATTACTATGAACTCAAGACTATTCTGAATAAGGCCGCT
	AGCTATTTACCCTTCCTTACGCAGTGCGGGAAGGATGCCATTTCTAACCAGGATAAACTC
	TTGAGTATAATGACATTTCGAATTCCCTATTTCGTGGGTCCGCTTCGTAAGGATAACAGT
	GAGCACGCTTGGCTGGAGCGGAAGGCTGGCAAAATTTATCCATGGAATTTCAACGACAAG
	GTGGATCTGGACAAATCCGAAGAAGCCTTTATCCGCAGGATGACCAATACTTGCACATAC
	TATCCTGGGGAGGATGTCCTTCCACTGGACTCTCTGATCTACGAAAAGTTCATGATTTTG
	AATGAAATTAACAACATAAGGATCGATGGGTATCCTATTTCCGTCGACGTGAAGCAGCAG
	GTGTTCGGGCTCTTTGAGAAGAAGCGACGGGTGACCGTGAAGGATATTCAGAATCTTCTC
	TTATCGCTGGGAGCCCTGGATAAACACGGAAAACTGACCGGGATAGATACTACGATTCAT
	TCTAATTACAACACGTATCACCATTTTAAGTCACTGATGGAGAGGGGCGTCCTAACAAGA
	GATGACGTGGAGAGAATAGTGGAACGAATGACATATTCTGATGACACCAAGAGAGTGCGG
	CTTTGGCTGAATAACAACTACGGCACTCTGACGGCGGATGATGTAAAGCATATTTCCCGA
	CTCCGTAAGCATGACTTCGGGCGGCTGTCTAAGATGTTTCTAACAGGCCTCAAGGGTGTG
	CATAAGGAAACTGGGGAGCGCGCTAGCATCCTGGATTTTATGTGGAACACCAATGATAAC
	CTGATGCAGCTCCTGTCAGAATGCTACACATTTTCGGACGAAATCACCAAGCTGCAGGAG
	GCTTACTATGCCAAGGCCCAACTAAGCTTGAATGATTTCCTGGATTCTATGTACATCAGC
	AACGCCGTAAAACGACCAATTTATAGGACACTGGCAGTGGTTAACGACATTAGGAAAGCA
	TGCGGAACAGCTCCCAAGCGAATCTTTATCGAGATGGCCCGCGACGGCGAGAGTAAGAAG
	AAAAGGTCAGTGACTAGGCGGGAGCAGATCAAGAACCTTTACCGCTCTATCCGAAAAGAC
	TTCCAGCAAGAGGTTGATTTCCTTGAGAAGATCTTAGAGAACAAGTCAGATGGACAGCTC
	CAATCCGATGCTCTGTATCTGTACTTCGCTCAGCTGGGACGAGATATGTACACTGGCGAC
	CCCATTAAACTAGAACATATCAAGGACCAATCGTTTTATAATATCGACCACATCTACCCT
	CAGTCCATGGTGAAAGACGATAGTCTGGACAATAAGGTGCTCGTCCAAAGTGAGATTAAC
	GGAGAAAAGTCGAGCAGATATCCTTTGGACGCTGCGATCCGCAACAAGATGAAGCCCCTG
	TGGGATGCTTACTACAATCATGGACTGATCAGCCTGAAGAAGTATCAGAGACTGACCCGG
	AGTACCCCTTTCACAGACGATGAGAAGTGGGATTTTATCAATAGACAACTGGTGGAAACC
	AGGCAGTCCACGAAAGCTCTGGCCATTCTTCTGAAGAGAAAGTTTCCAGACACAGAGATC
	GTCTATTCAAAGGCCGGCCTCAGTTCCGACTTTAGACATGAGTTCGGACTCGTTAAATCA
	CGAAATATAAACGATCTCCACCATGCAAAGGACGCATTCCTCGCGATTGTGACTGGAAAT
	GTCTATCACGAAAGATTTAATAGGCGGTGGTTCATGGTTAACCAGCCATACTCAGTGAAG
	ACCAAGACCCTTTTCACTCACTCTATTAAAAATGGCAACTTCGTGGCTTGGAATGGTGAG
	GAGGATCTTGGAAGAATTGTGAAGATGTTAAAACAGAATAAGAATACCATCCACTTTACT
	AGATTCAGCTTTGACCGAAAAGAGGGGCTATTCGATATTCAACCGTTAAAGGCTTCAACA
	GGTCTCGTTCCACGAAAGGCCGGACTGGACGTAGTGAAATACGGCGGCTATGATAAGAGC
	ACCGCAGCTTACTACCTCCTTGTGCGATTTACGCTCGAGGATAAGAAGACCCAACACAAG
	CTGATGATGATTCCCGTGGAGGGACTGTACAAAGCTCGAATTGACCATGATAAAGAGTTT
	CTCACAGATTACGCACAAACCACCATCTCTGAGATTCTCCAGAAAGACAAACAAAAAGTT
	ATAAACATAATGTTTCCAATGGGTACAAGGCATATTAAACTGAACAGCATGATCTCCATT
	GATGGCTTTTATTTGTCCATTGGAGGAAAGTCTAGTAAAGGCAAGTCTGTCCTCTGCCAT
	GCCATGGTACCCCTAATCGTCCCACACAAGATTGAATGCTACATCAAGGCTATGGAGAGT
	TTTGCTCGGAAATTTAAAGAGAATAATAAGCTGCGTATTGTGGAAAAATTCGACAAGATA
	ACCGTTGAAGACAATCTGAATCTGTACGAGCTCTTTCTGCAGAAGCTGCAGCATAACCCC
	TATAATAAGTTCTTCTCCACACAGTTCGATGTACTGACCAACGGGCGATCAACTTTCACA
	AAGCTAAGTCCTGAGGAACAGGTGCAAACACTCCTAAACATTCTTTCCATTTTTAAGACC
	TGCAGATCTTCAGGATGCGACTTGAAGAGCATTAACGGGAGCGCACAGGCAGCTAGGATC
	ATGATCTCAGCTGACCTGACAGGGCTGAGTAAAAAATACTCCGACATTCGGCTTGTAGAG
	CAAAGCGCCAGTGGGTTGTTCGTTAGTAAGTCGCAGAACCTGCTGGAATACCTGTAA

SEQ	ATGTCTTCTTTGACGAAGTTTACAAACAAATACTCTAAGCAGCTTACAATTAAGAACGAA
ID	CTGATTCCCGTAGGAAAGACTCTGGAAAACATCAAAGAGAATGGGCTGATAGACGGCGAC
NO:	GAACAACTGAATGAGAACTATCAGAAGGCCAAAATTATCGTGGATGACTTCCTGAGGGAT
148	TTTATTAACAAGGCCCTGAATAATACCCAGATCGGCAATTGGCGGGAACTGGCCGACGCT
	CTGAACAAAGAAGATGAGGACAATATCGAAAAATTACAAGACAAAATCAGGGGCATTATT
	GTCAGTAAGTTCGAGACATTCGATCTGTTCTCTTCGTACTCCATTAAGAAGGACGAGAAA
	ATCATCGATGATGACAATGACGTTGAGGAAGAAGAACTGGACTTGGGTAAAAAGACCTCA
	TCCTTCAAGTATATTTTTAAAAAAAATCTGTTTAAATTAGTGCTCCCCAGTTATTTAAAG
	ACAACTAACCAGGACAAGCTTAAGATTATCTCCTCTTTTGACAACTTTAGCACCTATTTT
	AGAGGCTTCTTTGAAAATCGCAAGAATATTTTCACTAAGAAGCCCATAAGCACCTCTATT
	GCCTACAGAATCGTACATGATAACTTCCCAAAATTTTTGGATAACATTAGATGTTTTAAT
	GTATGGCAGACCGAATGTCCTCAGTTAATTGTGAAGGCGGATAACTACCTCAAATCCAAG
	AATGTGATCGCCAAAGATAAGTCTCTTGCTAACTACTTTACGGTCGGAGCCTACGATTAC
	TTCTTATCTCAAAACGGTATTGACTTTTACAATAACATTATCGGGGGATTGCCTGCCTTC
	GCCGGCCATGAGAAAATTCAGGGCTTAAACGAGTTCATAAATCAGGAATGTCAAAAGGAC
	TCAGAGCTGAAATCAAAGCTTAAGAATCGACACGCATTTAAAATGGCGGTCTTGTTCAAA
	CAGATCCTCAGCGATAGAGAGAAAAGCTTCGTTATTGATGAATTCGAGAGCGACGCACAG
	GTGATTGATGCCGTGAAGAACTTCTATGCGGAACAGTGTAAAGACAATAATGTTATTTTC
	AACCTATTAAACTTGATTAAGAATATCGCGTTTTTAAGTGACGATGAACTCGACGGTATC
	TTTATAGAAGGCAAGTACCTGTCCTCTGTCAGCCAAAAACTCTACTCAGATTGGTCCAAG
	CTAAGAAATGACATCGAGGACAGTGCTAACAGCAAACAGGGCAATAAAGAGCTGGCAAAG
	AAAATCAAGACTAATAAAGGGGATGTGGAGAAGGCGATATCTAAATATGAGTTCTCCCTC
	TCCGAACTGAACTCCATCGTCCACGATAATACCAAGTTTAGTGATCTGTTGTCGTGTACA
	CTGCACAAAGTGGCCAGTGAAAAACTCGTCAAGGTGAACGAAGGCGATTGGCCCAAACAC
	CTGAAAAATAATGAGGAGAAACAGAAGATCAAAGAACCTTTGGATGCGTTGCTCGAAATA
	TATAACACACTGTTGATCTTCAACTGTAAAAGCTTCAACAAGAACGGGAACTTTTATGTA
	GACTACGATCGATGTATAAATGAACTGAGCAGCGTCGTTTACCTGTACAACAAGACTCGC
	AATTATTGTACGAAAAAACCATATAACACCGATAAGTTCAAGCTTAATTTCAACAGTCCC
	CAGCTGGGAGAAGGGTTCAGCAAATCAAAAGAAAACGATTGCCTGACATTACTCTTTAAA
	AAGGATGATAATTATTATGTTGGGATTATTAGGAAAGGCGCTAAGATCAACTTTGACGAC
	ACACAGGCCATAGCTGACAACACTGATAACTGCATCTTTAAAATGAATTACTTTCTGTTG
	AAGGACGCCAAAAAATTCATTCCAAAATGCTCTATTCAGCTCAAGGAGGTTAAGGCCCAT
	TTCAAGAAGTCTGAAGATGACTACATCCTCTCTGACAAGGAAAAATTCGCTAGTCCTCTG
	GTTATCAAAAAAAGTACCTTCTTGCTGGCTACAGCTCACGTGAAAGGCAAGAAAGGGAAC
	ATTAAGAAGTTCCAAAAGGAATACAGCAAAGAGAATCCAACCGAGTACAGAAATTCTCTG
	AACGAATGGATCGCATTCTGTAAAGAATTTCTAAAGACGTACAAGGCCGCTACCATTTTC
	GATATTACCACCTTGAAAAAAGCCGAGGAGTACGCCGACATCGTCGAATTCTATAAAGAC
	GTGGATAACCTGTGTTACAAATTGGAATTCTGCCCAATTAAGACCTCTTTCATTGAAAAC
	CTCATCGACAATGGGGACCTCTACTTATTTAGAATTAACAATAAGGATTTTTCTTCGAAA
	TCTACCGGAACTAAAAATCTGCACACACTGTATCTGCAAGCAATCTTCGATGAACGTAAT
	CTCAACAACCCTACAATAATGCTGAACGGCGGTGCTGAACTGTTCTACCGTAAAGAGAGT
	ATTGAACAGAAGAATCGAATCACACACAAAGCGGGCAGTATTCTCGTCAATAAGGTGTGC
	AAAGACGGGACCAGCCTGGACGATAAGATCAGGAATGAAATATATCAGTATGAGAACAAG
	TTTATCGACACCTTGTCGGATGAGGCAAAGAAGGTGCTACCTAACGTTATCAAGAAGGAA
	GCTACCCATGACATAACCAAGGATAAGCGGTTCACTTCTGACAAGTTCTTCTTCCACTGT
	CCTCTGACCATTAACTACAAGGAAGGAGACACTAAACAATTCAATAATGAAGTACTTAGC
	TTTTTGCGGGGTAATCCCGATATTAACATAATTGGTATCGACCGGGGAGAACGGAACCTG
	ATATACGTGACAGTAATTAATCAGAAAGGAGAAATCCTGGATTCCGTATCCTTCAATACC
	GTGACTAATAAATCTAGTAAAATCGAGCAGACGGTCGACTACGAGGAAAAGTTAGCAGTC
	AGAGAGAAGGAGAGAATCGAGGCCAAACGTTCCTGGGATAGTATCAGCAAGATTGCTACT
	CTGAAAGAAGGATATCTGTCCGCTATCGTCCATGAGATCTGTTTGTTGATGATCAAGCAC
	AATGCTATAGTGGTTCTGGAGAACCTGAACGCAGGCTTCAAGCGAATTAGAGGGGGCCTG
	TCGGAAAAAAGCGTTTACCAGAAGTTTGAAAAGATGCTAATCAATAAGTTAAATTACTTT
	GTAAGTAAAAAAGAAAGCGATTGGAATAAGCCATCAGGACTTTTAAACGGGCTGCAACTG
	AGCGACCAGTTTGAGTCATTCGAAAAACTGGGTATTCAGAGTGGTTTCATATTCTACGTA
	CCTGCCGCTTACACTTCAAAGATCGATCCTACAACTGGTTTTGCGAATGTCCTGAATCTG
	TCTAAGGTGAGGAATGTGGACGCAATCAAGTCTTTCTTCAGCAACTTCAACGAGATATCT
	TACAGCAAGAAAGAGGCTCTGTTTAAATTCAGTTTTGATCTGGATAGCCTGAGCAAGAAA
	GGATTCTCTTCTTTCGTAAAGTTTTCTAAGTCCAAATGGAACGTCTACACGTTCGGAGAG
	AGAATCATTAAACCAAAGAACAAGCAGGGGTATCGGGAAGACAAAAGGATCAATCTGACT
	TTCGAAATGAAGAAACTATTGAATGAGTACAAAGTCTCATTCGATTTGGAGAACAATCTG
	ATCCCCAATCTGACCAGCGCTAACCTCAAAGACACATTCTGGAAGGAGCTGTTTTTCATC
	TTTAAGACCACCCTGCAGCTACGGAATAGTGTCACAAATGGGAAAGAGGATGTACTGATC
	TCACCTGTGAAAAACGCCAAGGGGGAGTTCTTTGTGTCCGGCACCCATAACAAAACCCTG
	CCTCAGGACTGTGACGCGAACGGGGCCTACCACATCGCGCTAAAGGGGTTAATGATTCTC
	GAACGTAATAATCTGGTGCGCGAAGAAAAAGACACAAAGAAAATTATGGCCATCAGCAAC
	GTTGACTGGTTTGAGTACGTGCAGAAGCGTCGAGGAGTTTTGTAA

SEQ	ATGAACAACTATGACGAGTTCACTAAACTTTACCCCATTCAGAAAACCATCAGATTTGAA
ID	CTGAAGCCTCAGGGTCGTACCATGGAACACTTGGAAACTTTCAACTTTTTCGAGGAGGAC
NO:	AGGGATAGAGCTGAGAAATACAAGATCTTGAAAGAGGCCATCGACGAGTATCACAAAAAA
149	TTCATCGATGAGCATCTCACCAACATGTCGCTGGATTGGAACAGTCTCAAGCAGATTTCC
	GAGAAGTACTATAAATCTCGGGAGGAGAAAGATAAAAAGGTGTTTTTGAGCGAGCAAAAG
	CGAATGCGACAGGAGATAGTCTCTGAATTTAAGAAAGATGATCGGTTTAAAGACCTATTT
	TCCAAAAAGCTTTTTTCAGAGCTGCTGAAGGAAGAGATCTATAAAAAAGGCAATCACCAA
	GAAATTGATGCCCTGAAATCATTCGACAAATTCAGTGGGTATTTCATAGGACTGCATGAG
	AACCGGAAGAATATGTATAGTGATGGAGACGAGATCACAGCCATAAGCAATCGAATCGTT
	AACGAGAATTTCCCGAAGTTCCTGGATAACCTGCAGAAGTATCAAGAGGCTAGGAAAAAG
	TACCCTGAGTGGATCATCAAGGCTGAATCAGCTCTGGTGGCTCACAATATCAAGATGGAT
	GAAGTCTTTAGTCTTGAGTACTTTAATAAAGTCCTTAACCAGGAGGGCATCCAGCGCTAT
	AACCTGGCTCTCGGTGGCTACGTCACAAAAAGCGGAGAAAAGATGATGGGTCTCAACGAT
	GCACTGAATTTGGCTCATCAGTCGGAGAAGTCATCTAAGGGACGCATACACATGACACCA
	CTGTTTAAACAAATCCTGAGCGAAAAGGAATCATTTTCCTACATTCCCGACGTATTCACC
	GAGGACTCACAACTGCTGCCTAGTATAGGGGGGTTTTTCGCTCAGATAGAGAACGACAAA
	GATGGCAACATTTTTGACAGAGCCTTGGAGTTGATTTCATCTTACGCCGAGTACGATACG
	GAGCGCATTTATATTCGCCAGGCGGATATCAACAGGGTTTCCAATGTGATCTTTGGCGAG
	TGGGGAACGCTGGGCGGGCTGATGCGGGAATACAAAGCCGACTCGATCAATGACATCAAC
	CTGGAGAGAACATGCAAGAAGGTCGATAAATGGTTGGATAGCAAAGAGTTCGCCCTGAGT
	GACGTCTTGGAAGCTATCAAAAGAACCGGAAATAATGACGCGTTCAACGAGTATATCTCT
	AAAATGAGGACCGCGAGAGAAAAAATTGATGCAGCAAGGAAGGAGATGAAGTTTATATCT
	GAGAAGATCTCAGGCGATGAAGAGTCCATCCATATTATTAAAACTCTTCTGGACTCAGTG
	CAGCAATTCCTGCACTTTTTTAACCTCTTCAAGGCCAGGCAGGATATACCGTTAGACGGG
	GCTTTTTATGCCGAGTTTGATGAAGTTCATTCGAAACTTTTTGCTATAGTGCCTCTCTAT
	AATAAAGTTCGCAATTACCTGACAAAGAATAACTTAAACACAAAGAAAATCAAGCTCAAC
	TTCAAAAACCCAACACTGGCAAACGGATGGGATCAGAACAAGGTATATGATTACGCCTCA
	TTGATTTTCCTCCGGGACGGGAATTACTATCTGGGGATCATCAACCCTAAGCGCAAAAAG
	AACATTAAGTTCGAACAGGGATCTGGCAATGGTCCCTTCTATAGGAAAATGGTATACAAA
	CAGATTCCTGGCCCCAACAAGAATCTCCCACGCGTCTTTCTGACGTCCACTAAGGGAAAG
	AAGGAGTACAAGCCGTCTAAAGAAATTATCGAGGGCTATGAGGCAGACAAGCATATTAGG
	GGTGACAAGTTTGACCTAGACTTTTGTCATAAGCTTATCGACTTTTTCAAGGAGTCCATA
	GAGAAGCACAAAGATTGGTCAAAGTTTAATTTCTATTTTTCTCCAACAGAGTCCTACGGG
	GATATCTCTGAGTTCTATCTGGATGTTGAAAAGCAGGGGTACAGAATGCACTTCGAAAAT
	ATCTCAGCAGAAACTATCGATGAGTACGTAGAGAAAGGAGATCTGTTTCTTTTCCAAATC
	TACAATAAGGATTTTGTGAAGGCCGCCACTGGGAAGAAGGACATGCACACTATTTACTGG
	AACGCTGCATTTTCCCCTGAAAATCTGCAGGACGTAGTAGTGAAATTAAATGGTGAGGCA
	GAACTGTTTTACCGCGATAAATCAGACATCAAGGAAATAGTGCACCGGGAAGGCGAGATT
	CTTGTTAACCGAACATATAATGGCAGGACACCTGTCCCTGATAAAATTCATAAGAAACTG
	ACCGATTACCACAACGGTCGAACCAAGGATCTGGGCGAGGCCAAGGAATACCTCGATAAG
	GTGAGGTACTTCAAAGCCCATTATGACATCACCAAGGACCGAAGATACCTTAACGACAAA
	ATCTACTTCCATGTCCCACTCACCTTGAACTTCAAAGCTAACGGTAAGAAGAACCTCAAT
	AAAATGGTGATTGAAAAATTTCTGTCCGATGAGAAGGCCCATATCATCGGCATTGATCGC
	GGCGAGAGAAATCTCCTTTACTATTCTATCATTGATCGGTCGGGAAAGATTATCGACCAA
	CAATCACTGAATGTCATCGACGGATTCGACTATAGAGAGAAGCTGAACCAACGGGAAATC
	GAGATGAAGGACGCGCGCCAGTCCTGGAACGCTATCGGCAAAATTAAAGATTTGAAAGAA
	GGTTACCTCTCCAAAGCAGTGCACGAAATTACCAAAATGGCAATCCAGTACAATGCTATT
	GTGGTAATGGAGGAGTTAAATTACGGATTTAAGCGCGGGAGGTTCAAGGTTGAAAAGCAA
	ATTTACCAAAAATTTGAGAACATGTTGATTGATAAGATGAACTACCTGGTGTTCAAGGAC
	GCACCTGACGAGTCGCCAGGCGGCGTGTTAAATGCATATCAGCTGACAAATCCACTGGAG
	AGCTTTGCCAAGCTAGGAAAGCAGACTGGCATTCTCTTTTACGTCCCTGCAGCGTATACA
	TCCAAAATTGACCCCACCACTGGCTTCGTCAATCTGTTTAACACCTCCTCCAAAACCAAC
	GCACAAGAACGGAAAGAATTTTTGCAAAAGTTTGAGTCCATTAGCTACTCTGCCAAAGAC
	GGCGGGATCTTTGCTTTCGCATTCGACTACAGGAAATTCGGGACGAGTAAGACAGACCAC
	AAGAACGTCTGGACCGCGTACACTAATGGGGAACGCATGCGCTACATCAAAGAGAAAAAG
	AGGAATGAACTTTTTGACCCTTCAAAGGAAATCAAGGAAGCTCTCACCTCAAGCGGTATC
	AAATACGATGGCGGGCAGAATATTTTGCCAGATATCCTCAGATCGAACAATAATGGACTT
	ATCTATACTATGTACTCCTCCTTCATTGCAGCAATTCAAATGAGAGTGTACGATGGAAAG
	GAGGATTACATTATATCGCCAATTAAGAACTCCAAAGGCGAATTCTTCCGCACGGATCCT
	AAGCGAAGAGAACTCCCAATCGACGCTGATGCGAACGGCGCCTATAATATAGCCCTGCGG
	GGTGAATTAACAATGCGCGCTATTGCCGAGAAGTTCGACCCCGATTCAGAAAAAATGGCT
	AAGCTTGAGCTGAAACACAAAGATTGGTTCGAATTCATGCAGACAAGAGGCGACTAA

SEQ	ATGACTAAGACCTTCGATTCCGAGTTCTTCAACCTTTATTCCCTGCAGAAAACTGTAAGG
ID	TTTGAGCTGAAGCCGGTGGGCGAGACAGCCAGCTTCGTAGAGGATTTCAAGAATGAGGGT
NO:	CTCAAACGGGTAGTTAGTGAGGATGAGAGGAGAGCAGTGGACTATCAGAAGGTGAAAGAG
150	ATCATCGATGACTATCACCGGGATTTCATAGAGGAGTCGTTGAATTACTTCCCTGAGCAA
	GTATCCAAAGACGCGCTGGAACAGGCCTTTCATCTTTACCAGAAACTGAAGGCAGCGAAG
	GTTGAGGAGCGGGAAAAGGCCTTGAAAGAGTGGGAAGCCCTGCAGAAAAAGCTCAGAGAA
	AAGGTTGTCAAATGCTTCAGCGACAGCAACAAAGCCAGGTTCAGTAGGATCGATAAGAAA
	GAACTGATCAAAGAAGACTTGATCAATTGGCTGGTTGCACAGAACCGGGAAGATGATATT
	CCCACCGTAGAGACCTTCAACAACTTCACAACTTACTTCACCGGCTTCCATGAGAATCGT
	AAAAACATCTACAGTAAAGATGATCATGCAACCGCCATCTCCTTCCGGTTGATCCACGAG
	AATCTCCCCAAGTTCTTTGACAACGTGATAAGTTTCAATAAGTTGAAAGAGGGATTTCCC
	GAACTCAAGTTCGATAAAGTGAAGGAGGATCTGGAAGTGGATTATGACCTTAAGCACGCT
	TTCGAGATAGAGTACTTCGTGAACTTTGTGACTCAGGCCGGCATCGATCAGTATAACTAC
	CTCCTCGGGGGTAAGACGCTCGAGGACGGTACTAAGAAGCAAGGAATGAATGAGCAAATT
	AATCTATTTAAACAGCAGCAGACCAGGGATAAGGCTAGACAGATCCCCAAGCTTATTCCT
	CTTTTTAAACAGATCCTAAGTGAAAGGACAGAAAGTCAAAGCTTCATACCTAAGCAATTT
	GAAAGTGATCAGGAGCTGTTTGACTCCCTGCAAAAGCTGCACAACAATTGCCAGGACAAG
	TTTACCGTGCTGCAGCAGGCTATCCTCGGACTGGCTGAGGCGGATCTTAAGAAGGTATTC
	ATTAAGACTAGCGACCTCAATGCCCTTAGTAACACCATCTTTGGAAATTACTCCGTTTTC
	AGCGATGCCCTCAATCTATACAAAGAGAGCTTGAAGACTAAAAAAGCTCAGGAAGCTTTT
	GAAAAATTACCGGCACATTCTATACACGACCTTATACAATACTTAGAGCAGTTCAACAGC
	AGCCTCGACGCTGAGAAACAGCAATCCACAGACACCGTCCTGAATTACTTCATCAAAACC
	GATGAACTGTACTCCCGATTTATCAAGAGCACTTCAGAAGCCTTCACGCAAGTTCAGCCT
	CTGTTCGAGCTGGAGGCACTGTCCAGCAAGAGACGACCGCCAGAGTCTGAAGACGAGGGA
	GCCAAGGGTCAAGAGGGGTTTGAACAGATAAAGCGAATTAAGGCTTACTTGGATACTCTC
	ATGGAGGCGGTGCATTTCGCTAAGCCTTTGTACCTGGTTAAAGGCCGAAAAATGATTGAG
	GGGCTAGATAAGGATCAGTCTTTTTACGAGGCTTTTGAAATGGCCTACCAGGAATTGGAA
	TCCTTGATCATTCCAATCTATAATAAAGCCCGGAGTTATCTGAGCAGGAAGCCCTTCAAA
	GCCGACAAGTTCAAAATAAATTTTGACAATAATACGCTACTGTCTGGTTGGGACGCTAAC
	AAGGAAACAGCCAATGCTTCCATCCTGTTTAAGAAAGACGGCCTGTACTACCTGGGAATT
	ATGCCAAAAGGCAAAACTTTTTTGTTCGATTACTTTGTGTCATCAGAGGATAGCGAGAAG
	TTAAAGCAAAGACGGCAGAAGACCGCCGAAGAAGCCCTCGCACAAGACGGAGAATCATAT
	TTCGAGAAAATTCGATATAAGCTCCTGCCTGGCGCATCAAAGATGTTGCCAAAAGTCTTC
	TTTTCCAACAAAAACATCGGCTTTTATAACCCCAGCGATGATATCCTTCGCATCCGGAAC
	ACCGCCTCACATACCAAAAATGGAACTCCACAGAAGGGCCACTCGAAGGTTGAATTCAAC
	CTTAACGATTGTCACAAAATGATTGATTTTTTTAAGAGCTCCATTCAGAAACACCCCGAA
	TGGGGGTCCTTTGGCTTCACCTTTTCTGATACTTCAGACTTCGAGGACATGTCCGCCTTC
	TACAGGGAGGTGGAGAACCAGGGCTATGTCATCTCCTTCGACAAAATAAAAGAGACATAC
	ATTCAGAGCCAGGTCGAGCAGGGAAATCTGTACCTGTTTCAGATCTATAACAAGGATTTC
	AGTCCCTATAGCAAGGGCAAGCCCAATTTACATACCCTGTACTGGAAGGCCCTGTTCGAA
	GAGGCAAACCTTAACAATGTAGTTGCTAAGCTGAATGGGGAAGCAGAGATCTTCTTCCGA
	AGGCACAGCATCAAGGCAAGCGACAAAGTTGTACATCCTGCTAACCAGGCCATCGATAAC
	AAGAACCCGCATACAGAAAAGACACAGTCAACCTTTGAATACGACCTCGTGAAGGACAAG
	AGGTACACACAAGATAAATTCTTCTTCCACGTGCCCATCAGCTTGAATTTTAAAGCGCAG
	GGAGTGAGCAAATTTAACGACAAGGTCAACGGCTTCCTGAAGGGAAACCCCGACGTGAAT
	ATCATCGGAATTGATCGCGGTGAAAGACATCTCCTCTACTTTACTGTGGTGAACCAGAAG
	GGTGAGATCCTAGTACAGGAGAGCCTGAACACCCTTATGAGTGATAAGGGCCATGTGAAT
	GATTACCAGCAGAAGCTGGACAAGAAGGAACAGGAAAGGGACGCAGCGCGGAAGTCCTGG
	ACCACTGTTGAGAATATCAAAGAACTGAAGGAGGGATATCTTAGCCATGTGGTACACAAA
	CTTGCACATCTGATTATCAAGTATAATGCCATAGTCTGCCTGGAAGACTTGAACTTCGGT
	TTCAAGCGAGGAAGGTTTAAAGTGGAGAAGCAGGTGTACCAGAAGTTTGAGAAAGCCCTT
	ATTGATAAGCTAAACTACCTTGTCTTTAAGGAAAAAGAACTCGGCGAAGTTGGCCACTAT
	TTAACCGCCTACCAACTAACCGCCCCTTTCGAGTCTTTTAAGAAACTGGGAAAGCAGAGC
	GGAATACTCTTCTATGTGCCTGCAGACTACACCTCTAAGATCGACCCCACTACCGGCTTT
	GTAAACTTTCTAGATCTCCGCTATCAGTCAGTAGAAAAAGCCAAACAGCTCTTGTCAGAT
	TTTAACGCCATCCGATTTAATTCCGTCCAAAATTACTTCGAGTTCGAAATCGACTATAAA
	AAACTTACCCCCAAGAGAAAGGTTGGGACGCAGTCTAAGTGGGTAATCTGCACTTACGGT
	GACGTGAGATACCAGAACCGCCGAAACCAGAAAGGTCATTGGGAAACCGAGGAAGTGAAT
	GTGACTGAGAAGCTCAAGGCCCTCTTCGCTAGCGACAGTAAAACAACAACAGTTATCGAT
	TACGCCAATGACGATAATCTTATAGACGTGATCTTGGAACAAGACAAAGCCTCTTTTTTT
	AAGGAATTGTTGTGGTTGCTGAAACTTACAATGACCCTTAGGCACAGCAAGATCAAATCA
	GAGGATGACTTCATCCTCAGCCCGGTGAAGAATGAACAGGGAGAGTTCTACGATTCACGG
	AAGGCTGGAGAGGTGTGGCCCAAGGATGCCGACGCGAACGGGGCCTACCACATAGCTCTA
	AAAGGTCTGTGGAACCTGCAACAAATCAATCAATGGGAGAAAGGTAAGACACTGAACCTG
	GCCATCAAAAATCAAGATTGGTTCTCATTCATCCAGGAAAAGCCTTATCAAGAGTGA

SEQ	ATGCATACGGGAGGCCTTTTATCAATGGACGCAAAAGAGTTCACCGGGCAGTATCCATTA
ID	TCTAAGACACTCCGCTTCGAGCTGAGGCCCATTGGCAGGACCTGGGACAACCTGGAGGCG
NO:	TCGGGCTACCTGGCTGAGGACAGACATCGCGCAGAATGCTATCCGAGAGCTAAGGAGCTT
151	TTGGACGACAATCATCGCGCGTTCCTTAACCGGGTGCTCCCACAGATCGATATGGACTGG
	CACCCGATCGCTGAGGCTTTTTGCAAGGTCCATAAGAACCCTGGGAACAAAGAGCTCGCC
	CAGGACTACAACTTGCAGCTGAGCAAGCGACGGAAAGAGATTTCTGCCTACCTTCAAGAC
	GCCGATGGCTACAAAGGGCTCTTCGCAAAGCCCGCATTGGATGAGGCCATGAAAATCGCC
	AAGGAGAACGGGAATGAAAGTGACATCGAAGTTCTCGAAGCGTTTAACGGATTTAGCGTG
	TACTTTACCGGCTATCATGAGTCAAGGGAGAATATTTATAGCGATGAGGACATGGTCTCT
	GTGGCCTACCGGATTACCGAGGATAATTTCCCGAGGTTTGTTTCAAATGCACTAATATTC
	GACAAGTTAAATGAGAGCCACCCAGACATCATCTCGGAGGTCAGCGGCAACCTCGGAGTT
	GACGATATTGGCAAATACTTCGACGTGAGCAACTATAACAACTTCCTCTCACAGGCTGGC
	ATCGACGACTATAATCATATTATAGGCGGCCACACTACTGAGGATGGTCTCATTCAGGCA
	TTCAATGTAGTCTTGAATCTTAGGCACCAGAAGGACCCTGGGTTTGAAAAGATACAGTTC
	AAGCAGCTGTATAAGCAGATATTATCCGTGCGAACATCTAAAAGTTACATCCCCAAACAG
	TTTGATAACTCAAAGGAGATGGTGGATTGCATATGCGATTATGTGTCAAAAATTGAAAAG
	AGCGAGACTGTGGAGCGGGCTCTGAAGCTCGTCAGGAACATTAGCTCCTTTGACCTTAGA
	GGAATTTTCGTCAATAAAAAGAATCTGAGGATCCTGAGCAATAAGCTAATAGGAGATTGG
	GACGCCATAGAGACAGCATTGATGCATTCCAGCTCAAGCGAGAATGATAAGAAGTCTGTC
	TACGATAGCGCTGAAGCCTTCACGCTGGACGATATCTTCTCTTCCGTGAAAAAATTTAGT
	GATGCGTCCGCAGAAGATATCGGGAATCGAGCCGAAGATATCTGCAGGGTAATTTCAGAG
	ACCGCCCCTTTCATCAATGACCTGCGCGCCGTGGACCTGGATAGCCTGAATGACGATGGT
	TACGAAGCTGCAGTTTCTAAGATCAGGGAGTCTCTGGAGCCATATATGGACTTGTTTCAC
	GAACTTGAGATCTTTAGCGTGGGCGACGAGTTCCCGAAATGCGCAGCTTTCTATAGCGAG
	TTAGAGGAGGTCAGCGAGCAATTAATCGAGATCATACCCCTGTTTAATAAGGCACGGAGC
	TTTTGTACTCGCAAGCGCTACAGCACCGACAAGATTAAAGTTAATCTGAAATTTCCAACT
	CTCGCAGACGGGTGGGACCTAAACAAGGAACGCGATAATAAAGCCGCCATCCTTAGAAAG
	GACGGAAAGTACTATCTTGCCATCCTAGATATGAAAAAAGATCTGAGTTCCATTCGTACT
	AGCGATGAAGACGAATCTTCTTTCGAAAAAATGGAGTATAAGCTGCTCCCCTCGCCAGTC
	AAGATGCTACCCAAGATCTTTGTGAAGAGCAAAGCAGCCAAGGAAAAGTACGGGCTGACG
	GACAGGATGCTGGAGTGCTACGATAAGGGAATGCATAAATCAGGGTCAGCTTTTGACTTG
	GGCTTTTGCCATGAGCTAATCGATTACTACAAGCGCTGTATCGCCGAGTATCCAGGATGG
	GACGTTTTCGACTTTAAATTTCGGGAGACTTCTGATTATGGTTCAATGAAGGAGTTCAAC
	GAAGATGTCGCTGGTGCCGGTTACTACATGAGCCTTCGCAAGATTCCTTGTTCCGAAGTC
	TACCGGCTACTGGACGAGAAATCTATATATTTGTTCCAGATATATAACAAGGACTACAGT
	GAGAATGCACATGGGAATAAGAATATGCATACTATGTATTGGGAAGGTCTCTTTTCACCC
	CAAAATTTGGAGTCACCCGTGTTCAAACTTAGCGGTGGCGCAGAGCTGTTCTTTAGGAAA
	TCCAGTATACCCAATGACGCCAAGACAGTCCACCCAAAGGGTAGCGTCCTGGTGCCCAGA
	AACGATGTGAACGGCAGGAGAATCCCTGACAGCATTTACCGAGAACTTACCAGGTACTTC
	AACCGCGGCGACTGTAGAATCTCTGATGAGGCAAAGTCTTATCTGGATAAGGTGAAGACT
	AAGAAGGCAGATCATGACATTGTGAAAGACCGCCGCTTTACTGTCGACAAAATGATGTTT
	CACGTGCCTATCGCAATGAATTTTAAGGCAATCTCAAAACCGAATCTGAACAAGAAGGTG
	ATAGATGGCATTATCGATGACCAGGACCTCAAGATCATCGGAATCGACAGAGGTGAGCGA
	AACCTGATATACGTCACAATGGTAGATCGGAAGGGTAATATTCTGTACCAGGATTCACTA
	AACATCCTCAATGGATATGACTATCGAAAAGCTCTCGATGTCAGGGAATACGACAACAAG
	GAGGCGCGACGGAATTGGACAAAGGTGGAAGGCATACGGAAGATGAAGGAAGGCTATCTG
	TCACTAGCTGTCTCCAAATTGGCTGATATGATTATAGAGAACAACGCCATTATCGTGATG
	GAAGATCTCAACCATGGATTCAAGGCAGGAAGAAGTAAAATTGAGAAGCAGGTGTATCAG
	AAGTTCGAAAGCATGCTTATTAATAAGTTGGGTTATATGGTCTTAAAGGACAAGTCTATC
	GATCAGAGCGGCGGCGCACTCCATGGGTATCAGCTGGCTAACCATGTCACCACACTAGCA
	TCCGTAGGCAAACAGTGTGGCGTGATTTTCTACATTCCTGCTGCGTTCACTTCTAAGATC
	GATCCTACCACGGGATTCGCAGACCTGTTCGCACTGAGCAATGTTAAAAACGTGGCCTCC
	ATGAGGGAGTTCTTTAGCAAAATGAAAAGCGTGATTTATGACAAGGCCGAGGGCAAGTTC
	GCTTTCACATTTGACTACCTGGACTACAATGTGAAATCAGAGTGCGGGAGAACCCTGTGG
	ACCGTATACACGGTAGGGGAAAGATTCACTTACAGTCGAGTTAATCGGGAGTATGTCCGT
	AAAGTGCCAACTGACATCATCTACGATGCCCTTCAGAAGGCTGGCATAAGTGTTGAGGGG
	GATCTAAGGGACAGGATCGCTGAATCGGATGGCGATACTCTCAAATCAATCTTCTACGCC
	TTCAAGTATGCCCTCGACATGAGGGTAGAGAACCGGGAGGAGGACTATATACAGTCTCCC
	GTGAAGAATGCGTCGGGAGAGTTCTTCTGCTCAAAAAACGCCGGGAAATCTTTGCCGCAG
	GATTCTGATGCAAATGGGGCTTATAACATTGCTCTCAAAGGCATCCTGCAGCTGCGCATG
	CTATCTGAACAATATGACCCAAACGCTGAAAGCATTAGATTGCCATTGATCACCAATAAG
	GCTTGGCTGACTTTCATGCAGAGCGGTATGAAGACATGGAAAAACTAA

SEQ	ATGGATTCCCTTAAGGACTTCACAAATCTTTACCCCGTGAGTAAAACCCTGAGATTTGAA
ID	CTCAAGCCCGTGGGAAAGACTCTCGAGAATATCGAGAAGGCCGGGATTTTGAAGGAAGAC
NO:	GAGCATCGGGCGGAAAGTTACAGACGGGTGAAGAAGATTATAGATACTTATCACAAGGTC
152	TTTATAGACAGCTCTTTAGAGAACATGGCAAAGATGGGCATCGAGAACGAAATCAAGGCC
	ATGCTGCAGTCCTTCTGCGAGCTGTATAAAAAGGATCATCGGACCGAAGGCGAAGACAAG
	GCGCTGGATAAGATCAGGGCAGTGCTGCGCGGCCTCATTGTGGGTGCCTTCACTGGGGTG
	TGCGGGCGGAGAGAGAACACTGTGCAGAATGAGAAATACGAGAGTTTGTTCAAAGAGAAA
	CTCATCAAGGAAATCCTGCCCGACTTCGTCTTAAGCACAGAAGCCGAATCTCTCCCATTT
	TCTGTCGAGGAGGCCACGCGTTCCCTTAAAGAGTTCGACAGTTTCACTTCATACTTTGCC
	GGATTTTATGAAAACCGTAAAAATATATACTCCACTAAACCACAGTCAACTGCAATAGCT
	TACAGGTTAATCCACGAAAACCTGCCAAAATTCATCGACAATATACTCGTCTTTCAAAAA
	ATCAAGGAACCAATCGCGAAGGAACTTGAACACATCCGGGCTGACTTTAGTGCGGGAGGA
	TACATCAAAAAAGACGAGCGCCTGGAGGATATATTTTCACTAAATTATTATATTCATGTA
	CTGAGCCAGGCTGGCATAGAAAAGTACAACGCTCTAATTGGGAAAATCGTGACAGAAGGT
	GACGGGGAAATGAAAGGGCTAAACGAACATATTAACTTATATAACCAACAGCGGGGTCGA
	GAAGATCGTCTGCCCCTGTTCAGACCTCTGTATAAGCAAATACTCTCCGACAGAGAGCAG
	CTATCATATCTGCCCGAGTCCTTTGAGAAAGATGAAGAGCTGCTCCGGGCGCTCAAGGAG
	TTCTATGATCATATAGCCGAGGACATTTTGGGCAGAACTCAGCAACTCATGACGTCTATT
	TCTGAATATGATCTGTCTCGTATCTATGTCAGGAATGATAGCCAGCTGACCGATATATCC
	AAGAAGATGCTGGGGGACTGGAACGCCATTTATATGGCGAGGGAGCGAGCATACGATCAC
	GAGCAGGCACCCAAGAGAATCACAGCCAAATATGAGAGAGACCGCATTAAGGCGCTGAAG
	GGCGAAGAAAGTATCAGTCTGGCCAATCTGAACTCCTGCATAGCTTTCCTTGATAACGTG
	AGGGATTGCAGAGTTGATACTTACCTGAGTACCCTGGGCCAGAAGGAAGGGCCTCACGGC
	CTCTCTAATCTAGTGGAGAATGTATTTGCCTCCTACCACGAAGCTGAGCAGCTGCTGTCA
	TTTCCGTACCCAGAGGAAAATAATTTAATACAGGATAAGGACAACGTAGTGCTTATCAAA
	AATCTACTGGATAACATTTCCGACCTCCAGCGCTTTCTCAAACCACTTTGGGGGATGGGC
	GACGAGCCTGATAAGGATGAGCGCTTTTACGGCGAGTACAACTACATCAGGGGCGCCTTG
	GACCAGGTGATTCCCCTCTATAATAAAGTCAGGAATTACCTGACCCGAAAGCCATACAGT
	ACAAGAAAGGTGAAATTAAATTTCGGCAATAGTCAGCTGCTGTCTGGTTGGGACCGAAAT
	AAGGAGAAAGACAACAGCTGCGTAATTCTCAGAAAAGGACAGAACTTTTATTTGGCCATC
	ATGAATAACAGACACAAGAGATCTTTCGAGAACAAAGTGCTCCCTGAGTATAAGGAGGGG
	GAACCCTACTTCGAGAAGATGGACTATAAATTCCTTCCTGATCCAAATAAAATGCTGCCT
	AAAGTATTTCTGTCAAAAAAAGGTATAGAAATCTACAAACCTTCACCTAAGCTACTTGAA
	CAGTATGGCCACGGCACCCATAAAAAAGGGGACACGTTCAGCATGGACGACCTACACGAA
	CTGATTGACTTCTTTAAGCACAGCATAGAAGCTCATGAGGACTGGAAACAGTTCGGATTC
	AAATTCTCAGATACCGCGACCTACGAAAACGTGTCTAGTTTTTACCGGGAAGTCGAGGAC
	CAGGGCTACAAGCTCAGCTTCAGAAAAGTTAGCGAATCTTACGTCTACTCCCTTATAGAT
	CAAGGTAAGCTGTATCTCTTTCAAATCTACAACAAGGACTTTTCCCCATGTAGCAAGGGC
	ACCCCCAATCTGCACACTCTCTACTGGCGGATGCTGTTCGACGAGCGTAACCTGGCAGAC
	GTGATCTACAAATTAGATGGTAAAGCTGAGATCTTCTTTCGTGAAAAGAGCCTAAAGAAC
	GATCACCCCACTCACCCCGCCGGAAAGCCCATTAAGAAGAAAAGTAGGCAGAAGAAAGGA
	GAAGAATCGCTATTTGAGTACGACCTCGTCAAGGATCGGCATTATACAATGGATAAGTTC
	CAGTTCCATGTGCCAATAACTATGAATTTCAAGTGCAGTGCTGGCAGTAAGGTGAATGAC
	ATGGTAAACGCTCATATCCGGGAGGCAAAGGACATGCATGTTATTGGAATTGATAGGGGT
	GAGCGTAATCTCCTCTACATCTGTGTTATTGACTCCCGCGGCACAATCCTCGATCAGATT
	TCCTTGAATACAATTAATGATATAGACTACCATGACTTGCTTGAGTCTCGCGACAAAGAT
	AGACAGCAGGAGAGAAGAAATTGGCAGACCATCGAAGGCATCAAGGAACTCAAGCAAGGC
	TACCTTTCTCAGGCAGTGCATCGAATAGCCGAGCTGATGGTGGCTTATAAAGCCGTCGTG
	GCACTAGAAGACCTAAATATGGGATTTAAACGAGGCAGGCAGAAGGTGGAATCATCCGTA
	TACCAGCAGTTCGAAAAACAGTTGATAGACAAACTCAATTACCTTGTAGACAAGAAGAAG
	CGGCCTGAGGACATAGGGGGCCTGCTTAGAGCGTATCAATTTACAGCCCCATTCAAGTCT
	TTCAAAGAAATGGGTAAACAGAACGGTTTTCTGTTTTACATCCCAGCGTGGAACACCAGC
	AATATAGATCCAACCACTGGCTTCGTCAATCTGTTTCATGCTCAGTATGAAAATGTGGAC
	AAGGCCAAATCCTTCTTTCAGAAATTTGACAGCATCTCCTATAACCCAAAGAAAGACTGG
	TTTGAATTCGCCTTTGACTATAAGAATTTCACTAAGAAGGCCGAGGGATCAAGAAGCATG
	TGGATATTGTGCACGCATGGCTCACGTATAAAGAACTTTAGAAACTCGCAAAAAAACGGG
	CAGTGGGACTCAGAAGAATTCGCACTCACCGAGGCTTTCAAATCCCTCTTCGTCCGGTAT
	GAGATCGATTACACCGCCGATCTGAAGACGGCAATCGTCGACGAGAAACAGAAAGACTTC
	TTTGTAGATCTACTTAAGCTCTTTAAGCTAACCGTTCAGATGCGAAACAGTTGGAAAGAA
	AAGGATCTCGACTATCTCATTAGTCCAGTGGCTGGCGCGGATGGTAGATTTTTCGATACC
	CGGGAAGGTAACAAGTCCCTTCCCAAAGACGCCGACGCGAATGGTGCCTACAATATTGCA
	CTAAAGGGGCTCTGGGCGCTGCGGCAAATTAGACAGACATCTGAAGGGGGCAAGCTTAAG
	CTGGCTATTTCTAATAAAGAGTGGTTGCAGTTTGTGCAGGAAAGGAGTTATGAGAAGGAC
	TAG

SEQ	ATGAACAACGGCACCAACAACTTCCAGAACTTCATCGGCATATCGTCTCTGCAGAAAACA
ID	CTTAGGAATGCCCTGATTCCAACTGAGACAACACAGCAGTTTATTGTGAAGAATGGGATC
NO:	ATCAAAGAGGACGAATTGCGCGGGGAGAATAGGCAGATCCTGAAGGACATCATGGACGAT
153	TACTACAGGGGTTTTATCTCCGAAACGCTGAGCTCGATTGACGATATTGACTGGACGTCC
	CTCTTTGAGAAGATGGAAATCCAACTTAAAAATGGCGATAATAAAGATACCCTGATAAAG
	GAACAAACCGAATATAGAAAGGCTATACACAAAAAATTCGCAAATGACGACCGCTTTAAG
	AACATGTTTTCTGCAAAACTGATTAGCGATATTCTGCCCGAGTTTGTGATTCACAATAAT
	AACTATTCCGCTTCGGAGAAGGAGGAAAAGACTCAGGTGATTAAACTGTTTTCTCGGTTC
	GCCACTTCTTTCAAAGATTATTTCAAAAATCGCGCCAACTGTTTTTCCGCTGACGACATC
	TCCTCCTCTTCCTGCCACCGGATCGTAAACGACAATGCCGAGATCTTTTTTAGTAACGCC
	CTTGTGTATCGGAGGATAGTGAAGAGCCTGTCCAATGATGACATAAACAAAATTTCTGGC
	GATATGAAGGATAGCCTCAAAGAGATGAGCCTTGAAGAAATTTACTCCTACGAGAAGTAT
	GGGGAGTTCATCACCCAGGAGGGGATTTCCTTCTATAATGACATCTGTGGCAAGGTGAAC
	AGCTTCATGAACCTGTACTGCCAGAAGAATAAGGAAAACAAAAATCTGTACAAGCTTCAG
	AAGTTACATAAGCAGATCCTGTGTATCGCGGATACCTCATATGAGGTTCCTTATAAGTTC
	GAGAGTGATGAAGAAGTGTACCAGTCTGTAAATGGATTCTTAGACAATATTTCGTCCAAA
	CATATAGTGGAGAGACTGAGAAAGATCGGGGACAATTACAATGGGTACAATCTCGACAAG
	ATTTATATCGTGTCGAAGTTTTACGAATCTGTGAGCCAGAAAACATACAGGGATTGGGAA
	ACCATTAATACCGCGCTTGAAATTCACTACAATAATATTCTGCCTGGCAACGGAAAAAGC
	AAGGCCGATAAGGTAAAAAAGGCAGTCAAAAATGACCTTCAGAAAAGTATCACCGAAATC
	AATGAGTTGGTGAGCAACTACAAATTGTGTTCAGACGATAATATTAAAGCGGAAACGTAC
	ATACATGAAATTAGCCATATTCTGAATAACTTTGAGGCGCAGGAACTTAAGTACAACCCT
	GAAATTCATCTCGTCGAAAGCGAATTGAAGGCCTCTGAATTGAAAAACGTTCTTGACGTG
	ATAATGAACGCTTTCCATTGGTGCTCTGTGTTTATGACTGAAGAGCTGGTTGATAAGGAC
	AACAACTTTTATGCTGAACTTGAGGAAATCTACGACGAGATCTACCCTGTGATTAGCTTG
	TATAACCTCGTCAGAAACTACGTTACCCAGAAGCCGTACAGCACGAAAAAAATAAAGCTG
	AACTTTGGTATTCCGACTCTCGCCGATGGATGGAGCAAGTCGAAGGAATATTCCAACAAT
	GCCATCATTCTTATGCGAGACAATCTGTATTACCTCGGCATCTTTAACGCCAAAAACAAG
	CCGGATAAGAAAATCATTGAAGGGAATACGAGCGAGAATAAGGGCGACTATAAGAAAATG
	ATCTACAACTTACTGCCAGGTCCCAATAAAATGATTCCTAAGGTGTTTCTGTCATCGAAA
	ACAGGTGTAGAAACATATAAGCCCAGCGCATACATCCTGGAAGGCTACAAGCAAAACAAA
	CACATCAAAAGCAGCAAGGACTTTGATATCACATTCTGCCACGATCTAATCGACTACTTC
	AAAAATTGCATCGCCATTCACCCTGAGTGGAAGAACTTCGGCTTTGACTTCTCCGACACC
	AGTACCTACGAAGACATTTCTGGATTCTACCGTGAGGTTGAGCTGCAGGGTTATAAAATT
	GACTGGACATACATCAGTGAAAAAGACATCGATCTACTGCAGGAGAAGGGGCAGCTCTAT
	CTCTTCCAGATTTATAATAAGGATTTCAGCAAGAAGTCCACTGGAAACGACAATCTGCAT
	ACAATGTATCTTAAGAACTTGTTTAGCGAAGAGAATTTGAAAGATATCGTTCTAAAGTTA
	AACGGGGAAGCCGAGATTTTCTTTCGAAAGTCTTCCATTAAGAATCCAATTATTCACAAG
	AAGGGCAGTATCCTGGTCAACAGAACCTATGAGGCCGAGGAAAAGGACCAGTTCGGTAAT
	ATACAAATTGTGCGCAAGAACATCCCCGAGAACATTTACCAGGAGCTCTATAAATACTTC
	AACGACAAAAGCGATAAGGAGCTTTCCGACGAGGCTGCCAAGCTGAAAAACGTGGTGGGA
	CACCATGAAGCAGCCACCAACATCGTCAAAGATTATCGTTATACATATGACAAATATTTT
	CTGCACATGCCTATTACAATAAACTTTAAGGCAAACAAGACCGGGTTCATCAATGACCGG
	ATACTCCAGTACATCGCAAAAGAGAAGGACCTGCATGTGATCGGCATCGACCGCGGTGAA
	AGAAATCTCATTTACGTCAGCGTTATCGACACTTGTGGAAACATTGTGGAGCAGAAGTCC
	TTCAACATTGTTAACGGCTATGACTATCAGATCAAGCTCAAACAGCAGGAAGGTGCTCGT
	CAGATTGCGAGGAAAGAATGGAAAGAGATCGGCAAGATCAAGGAGATCAAAGAAGGGTAT
	CTGAGCTTGGTCATTCACGAGATCTCCAAAATGGTCATCAAGTACAACGCTATTATCGCG
	ATGGAAGACCTCTCTTACGGCTTTAAGAAGGGGCGCTTTAAAGTGGAGCGCCAGGTCTAT
	CAGAAGTTCGAGACTATGCTTATCAATAAGCTGAATTACTTGGTCTTTAAGGATATCAGT
	ATCACCGAGAACGGAGGACTGCTGAAAGGTTACCAGCTCACATATATTCCCGATAAGCTC
	AAGAATGTGGGCCACCAATGCGGTTGTATTTTTTACGTTCCAGCTGCCTACACATCTAAG
	ATCGATCCTACCACCGGATTCGTCAATATATTTAAATTTAAAGATCTAACCGTTGATGCC
	AAGCGTGAGTTTATTAAGAAATTTGATTCAATCAGGTACGACAGCGAAAAGAACCTCTTC
	TGTTTCACTTTCGACTACAACAACTTCATCACACAAAATACTGTGATGAGCAAGTCATCA
	TGGAGCGTTTATACTTATGGTGTAAGGATAAAAAGGCGCTTTGTTAATGGAAGGTTTTCC
	AATGAAAGCGATACAATAGACATCACAAAAGACATGGAGAAGACACTGGAGATGACAGAT
	ATTAATTGGAGGGACGGGCATGACCTTAGACAGGACATCATCGACTACGAAATCGTCCAA
	CACATTTTTGAGATATTCAGACTCACTGTCCAGATGCGAAACAGCCTGTCGGAACTCGAA
	GACCGGGACTACGATAGACTGATCTCCCCGGTGTTAAACGAAAATAATATTTTCTACGAT
	TCTGCTAAGGCAGGAGACGCTCTTCCTAAAGATGCGGACGCCAATGGCGCTTACTGTATA
	GCGTTGAAGGGATTGTATGAGATTAAACAGATCACTGAGAATTGGAAAGAAGACGGTAAA
	TTCTCCAGAGACAAGCTGAAAATCTCCAACAAAGACTGGTTTGATTTTATTCAAAATAAG
	CGCTACCTGTAA

SEQ	ATGACAAACAAATTTACTAATCAGTACAGCCTGTCAAAGACCCTCCGCTTCGAACTGATT
ID	CCACAAGGGAAGACCCTTGAATTCATCCAGGAAAAGGGTTTATTATCCCAGGATAAACAA
NO:	CGCGCAGAAAGCTATCAAGAGATGAAGAAGACGATCGATAAATTTCATAAGTATTTCATA
154	GATTTAGCCCTGAGCAACGCTAAATTGACCCACCTGGAAACCTATTTGGAGCTGTACAAC
	AAGTCAGCCGAGACAAAGAAAGAGCAGAAGTTTAAGGACGACCTGAAAAAAGTACAGGAC
	AATTTGCGAAAAGAGATCGTCAAGTCTTTTTCCGACGGAGACGCCAAGTCAATATTTGCC
	ATCCTGGACAAAAAGGAACTCATCACTGTGGAGTTGGAGAAGTGGTTTGAGAATAATGAG
	CAGAAGGACATCTATTTTGACGAAAAGTTCAAGACATTTACTACTTACTTCACCGGATTT
	CACCAAAACCGGAAGAACATGTACTCTGTTGAGCCGAACTCAACCGCCATCGCCTACCGC
	CTTATTCACGAAAATCTGCCAAAGTTTCTCGAGAATGCTAAAGCCTTTGAGAAAATTAAG
	CAGGTCGAGTCGCTCCAGGTGAACTTTCGAGAGCTGATGGGTGAATTCGGGGACGAGGGC
	CTGATTTTCGTGAATGAACTCGAAGAGATGTTTCAGATCAACTACTATAATGATGTACTC
	TCACAGAACGGGATCACTATCTACAACAGCATTATCTCTGGATTCACTAAGAACGATATC
	AAGTATAAAGGGCTGAATGAATACATCAACAATTATAATCAGACTAAGGACAAAAAGGAC
	AGGCTGCCTAAATTGAAACAGCTGTATAAGCAGATCCTCAGTGATAGAATTAGCTTGTCA
	TTTCTCCCAGATGCCTTCACTGACGGAAAGCAGGTGCTTAAGGCGATATTCGATTTCTAT
	AAGATCAACCTCCTCTCTTATACAATCGAGGGCCAGGAGGAGTCACAGAACCTCCTGCTC
	CTGATTCGACAAACTATTGAAAATCTGTCCTCTTTCGATACGCAGAAGATATACCTGAAA
	AATGACACCCATCTCACTACAATATCCCAACAGGTATTCGGAGATTTCTCCGTCTTCAGT
	ACAGCCCTGAATTACTGGTACGAGACAAAGGTGAACCCTAAGTTCGAAACAGAGTACAGC
	AAGGCGAACGAAAAGAAGAGGGAGATCCTGGACAAAGCCAAAGCCGTTTTCACCAAGCAA
	GATTACTTTAGCATCGCATTTCTGCAGGAAGTCCTGTCTGAGTACATACTGACACTCGAT
	CACACAAGCGACATAGTTAAGAAGCACTCTTCCAATTGTATCGCGGACTACTTCAAAAAT
	CATTTTGTCGCGAAAAAGGAGAACGAGACAGATAAGACCTTCGATTTTATCGCGAATATT
	ACCGCAAAGTATCAATGCATTCAGGGTATCTTGGAGAACGCCGACCAGTACGAAGACGAG
	CTTAAACAGGATCAGAAGCTCATCGACAACCTAAAGTTCTTTTTGGACGCTATACTGGAA
	CTCCTTCATTTTATTAAGCCACTACATCTGAAGAGTGAGTCTATCACTGAGAAGGACACT
	GCTTTTTACGACGTTTTCGAGAATTACTACGAAGCACTGTCTCTGCTAACCCCTCTGTAT
	AACATGGTGAGAAACTATGTGACACAGAAACCTTATAGTACCGAGAAGATTAAGTTGAAC
	TTCGAGAACGCACAATTGCTGAATGGGTGGGATGCAAACAAAGAGGGTGATTACCTCACA
	ACAATCCTCAAGAAAGATGGCAATTACTTCCTGGCCATTATGGATAAAAAACATAACAAG
	GCATTTCAGAAATTTCCCGAGGGGAAGGAAAATTATGAAAAGATGGTATACAAGTTGCTG
	CCCGGGGTGAACAAAATGCTCCCGAAGGTGTTTTTCTCGAATAAGAATATCGCGTACTTT
	AACCCGTCCAAGGAACTGTTGGAAAATTATAAAAAGGAAACACACAAGAAGGGGGACACT
	TTTAATTTGGAGCACTGCCACACACTCATTGACTTCTTTAAAGATAGTCTCAACAAACAT
	GAGGATTGGAAATATTTTGACTTTCAGTTTAGCGAGACCAAGTCTTATCAGGATCTGTCG
	GGATTTTATAGGGAAGTTGAGCACCAGGGTTACAAGATAAATTTCAAGAACATCGATAGC
	GAGTACATTGACGGACTGGTGAACGAAGGGAAGCTGTTCCTGTTTCAGATTTACAGCAAA
	GATTTCTCTCCTTTCTCAAAAGGCAAGCCGAACATGCATACCCTGTATTGGAAGGCCCTG
	TTCGAGGAGCAAAACCTTCAGAATGTGATTTACAAGCTGAACGGTCAGGCCGAGATTTTT
	TTTAGGAAGGCCTCTATCAAGCCCAAAAACATCATTCTGCACAAGAAAAAGATAAAGATC
	GCCAAAAAACACTTCATTGATAAAAAGACAAAGACTTCTGAGATCGTACCTGTTCAGACA
	ATCAAGAATCTCAACATGTATTATCAGGGGAAGATTAGCGAGAAAGAGCTGACACAGGAC
	GATTTGAGGTACATCGACAACTTCTCTATCTTTAACGAGAAGAACAAGACAATCGATATC
	ATCAAGGACAAGCGGTTTACCGTCGATAAATTCCAGTTCCATGTGCCTATCACGATGAAT
	TTCAAGGCCACCGGTGGGAGTTATATCAACCAGACTGTGCTGGAGTATCTGCAGAACAAC
	CCCGAAGTAAAAATTATTGGCCTGGACAGAGGAGAGCGGCATCTGGTGTACTTGACCCTC
	ATCGATCAGCAGGGAAATATCCTGAAACAAGAATCTCTGAATACTATTACGGACTCCAAA
	ATCAGCACACCTTACCACAAGCTGCTTGATAATAAAGAGAATGAGAGGGACTTGGCCCGC
	AAAAATTGGGGCACCGTCGAGAATATTAAGGAATTGAAAGAAGGATACATCTCACAGGTG
	GTTCACAAAATCGCAACCCTGATGTTAGAAGAGAACGCTATTGTGGTGATGGAGGACTTA
	AACTTCGGATTTAAAAGAGGAAGATTTAAAGTCGAGAAACAGATTTATCAGAAACTGGAA
	AAAATGCTCATTGACAAATTAAATTACCTGGTGCTGAAAGATAAACAGCCACAGGAGCTG
	GGTGGCCTGTATAATGCTCTGCAGCTGACCAACAAGTTCGAGTCGTTTCAGAAAATGGGC
	AAGCAGTCAGGCTTCCTTTTTTACGTGCCCGCTTGGAACACCTCAAAAATCGACCCTACA
	ACAGGCTTTGTGAATTATTTCTATACCAAGTATGAAAACGTGGACAAGGCAAAGGCCTTT
	TTCGAGAAGTTTGAAGCAATCAGGTTCAATGCCGAGAAAAAATACTTTGAGTTCGAGGTC
	AAAAAATATAGCGACTTCAACCCTAAGGCCGAAGGCACGCAACAAGCCTGGACAATATGC
	ACGTATGGGGAGAGAATTGAGACTAAGCGGCAGAAGGATCAGAATAACAAATTCGTGAGC
	ACACCGATTAACCTGACAGAGAAGATAGAGGACTTCCTCGGCAAGAATCAGATCGTGTAC
	GGCGACGGCAATTGCATCAAGTCACAAATTGCATCTAAAGATGACAAAGCATTCTTCGAA
	ACACTGCTGTATTGGTTCAAGATGACACTCCAGATGCGAAATAGCGAAACAAGAACAGAT
	ATTGACTACCTCATCAGCCCTGTGATGAATGATAACGGCACGTTTTACAATTCCCGGGAC
	TATGAAAAATTAGAGAACCCGACACTGCCAAAAGACGCCGACGCAAATGGTGCATATCAC
	ATCGCAAAGAAAGGTTTGATGCTGTTGAACAAAATTGATCAGGCTGATCTGACAAAAAAG
	GTCGATCTGAGTATCAGTAACCGCGACTGGTTGCAGTTTGTCCAGAAGAACAAATAA

SEQ	ATGGAACAAGAGTACTATCTGGGCCTGGACATGGGCACCGGGAGTGTCGGATGGGCAGTC
ID	ACCGACTCAGAGTACCACGTCCTCAGAAAGCACGGTAAGGCACTTTGGGGAGTGCGACTC
NO:	TTCGAGTCCGCTAGTACTGCTGAAGAGAGGAGGATGTTTCGAACTTCCAGGCGCAGGCTG
155	GATCGGCGAAACTGGAGAATAGAGATTCTCCAGGAGATATTTGCTGAAGAGATTTCAAAG
	AAGGATCCTGGTTTTTTCCTGCGCATGAAAGAATCTAAGTATTACCCCGAAGATAAACGC
	GACATCAACGGCAATTGTCCTGAACTGCCCTATGCTCTGTTTGTCGACGACGATTTCACC
	GACAAAGATTACCACAAGAAATTCCCCACCATATACCACCTGAGAAAGATGTTGATGAAC
	ACCGAGGAGACACCCGACATACGTCTGGTTTACCTGGCTATCCATCATATGATGAAGCAC
	CGCGGGCATTTCCTGCTGTCTGGAGACATCAATGAGATAAAGGAATTTGGTACTACGTTC
	TCCAAGTTGTTAGAAAACATTAAGAATGAAGAGTTGGACTGGAATCTTGAACTGGGAAAG
	GAAGAGTATGCAGTTGTAGAGTCGATTTTGAAAGATAACATGTTAAACCGGTCAACTAAG
	AAAACCAGGTTAATTAAGGCACTAAAGGCCAAATCGATATGCGAGAAGGCTGTGCTAAAT
	CTGCTGGCTGGAGGCACCGTGAAACTGTCTGATATTTTCGGCCTGGAAGAGCTCAATGAA
	ACCGAGCGGCCTAAAATTTCTTTCGCCGATAACGGATACGATGACTATATTGGGGAGGTG
	GAAAACGAGCTCGGAGAACAATTCTACATTATTGAAACCGCTAAGGCAGTCTATGACTGG
	GCCGTGCTCGTCGAGATTTTAGGCAAGTACACCAGCATTAGCGAAGCAAAGGTGGCTACC
	TATGAAAAGCACAAATCTGACCTCCAGTTTCTGAAAAAGATTGTGCGCAAATACTTAACA
	AAAGAAGAGTACAAGGACATCTTTGTGAGCACATCAGATAAGCTCAAGAATTACTCAGCA
	TACATTGGAATGACAAAGATTAACGGGAAGAAGGTGGATCTCCAAAGCAAACGTTGTTCA
	AAGGAGGAGTTTTACGATTTCATAAAGAAGAACGTGCTGAAGAAACTGGAGGGACAACCG
	GAGTACGAGTATTTAAAGGAGGAGCTCGAGCGAGAAACTTTCCTGCCCAAGCAAGTGAAC
	AGAGACAATGGTGTCATTCCTTACCAGATTCACTTATATGAGCTGAAGAAAATCCTGGGG
	AACTTGAGAGACAAGATAGACCTCATCAAGGAAAATGAAGATAAGTTGGTCCAGTTGTTC
	GAATTCAGAATCCCATATTACGTCGGCCCGCTCAATAAGATCGACGACGGCAAGGAAGGC
	AAATTCACTTGGGCGGTGCGAAAAAGCAACGAAAAAATATACCCATGGAACTTTGAGAAC
	GTCGTTGACATCGAGGCCAGCGCCGAGAAATTTATAAGACGCATGACTAATAAGTGTACT
	TACCTCATGGGCGAGGATGTTCTGCCCAAGGACAGCCTGCTGTATTCCAAGTACATGGTG
	CTTAACGAGCTGAATAATGTAAAGTTAGATGGTGAGAAGCTCAGCGTGGAGCTTAAACAG
	AGGCTGTACACTGATGTGTTTTGCAAGTATCGGAAAGTTACCGTTAAGAAGATAAAGAAT
	TACCTGAAATGCGAAGGGATCATTTCCGGCAACGTGGAAATTACCGGAATCGACGGCGAT
	TTTAAGGCGTCGTTGACCGCTTATCATGATTTCAAGGAGATTTTAACCGGCACGGAGCTC
	GCGAAGAAAGACAAGGAGAACATAATCACGAATATAGTTCTGTTTGGGGACGATAAAAAA
	CTTCTTAAAAAACGACTCAATCGACTGTATCCGCAGATTACCCCCAACCAGCTGAAGAAG
	ATTTGCGCTCTGAGCTATACCGGGTGGGGCCGGTTCTCTAAGAAATTCCTCGAGGAGATC
	ACAGCACCAGACCCAGAGACTGGTGAGGTGTGGAATATTATTACAGCTCTGTGGGAATCC
	AATAATAACCTTATGCAATTGTTGAGCAATGAATATAGGTTCATGGAGGAAGTGGAAACC
	TACAATATGGGCAAGCAGACAAAGACCCTATCTTACGAGACCGTTGAGAATATGTATGTC
	TCCCCTTCAGTGAAACGGCAAATCTGGCAAACTTTGAAGATCGTGAAGGAGCTCGAAAAG
	GTGATGAAAGAGAGCCCGAAGAGGGTTTTTATTGAAATGGCCAGAGAGAAACAGGAGAGC
	AAGAGAACAGAGTCTAGGAAGAAGCAGCTAATCGATTTGTATAAAGCCTGCAAGAACGAG
	GAAAAAGACTGGGTCAAGGAGCTAGGCGATCAGGAAGAACAGAAGTTGCGCTCTGATAAG
	CTGTACTTATATTATACCCAGAAAGGACGGTGCATGTACTCAGGTGAGGTCATTGAGCTG
	AAAGATCTGTGGGACAATACTAAGTATGATATTGATCACATCTACCCTCAGTCAAAAACT
	ATGGACGACTCCCTCAACAACAGGGTGTTGGTTAAGAAGAAATACAATGCTACAAAGTCC
	GATAAATACCCTCTTAACGAAAACATCCGGCACGAAAGAAAGGGCTTCTGGAAGTCCCTG
	CTGGATGGGGGTTTTATCAGTAAAGAAAAGTATGAGAGGCTGATCCGAAATACCGAGCTC
	TCCCCCGAGGAACTGGCTGGCTTTATCGAAAGGCAGATCGTAGAGACTAGGCAATCTACA
	AAGGCAGTCGCTGAGATCCTGAAGCAAGTGTTTCCTGAGTCAGAAATCGTGTACGTCAAA
	GCTGGCACAGTGTCACGGTTCCGAAAGGACTTTGAGTTGTTAAAAGTTCGGGAGGTGAAT
	GACCTGCACCACGCTAAAGACGCCTATCTGAATATCGTTGTGGGGAACTCCTATTATGTT
	AAGTTTACTAAGAATGCGTCCTGGTTTATTAAGGAGAACCCGGGGCGCACCTATAACCTG
	AAGAAGATGTTCACCTCCGGCTGGAACATAGAACGGAACGGAGAAGTCGCGTGGGAGGTG
	GGTAAGAAAGGGACCATTGTGACCGTCAAACAGATTATGAACAAAAACAACATATTGGTA
	ACTCGCCAGGTGCATGAGGCCAAAGGGGGCCTCTTTGATCAGCAGATTATGAAAAAGGGC
	AAAGGACAGATCGCAATCAAGGAAACCGACGAGCGCCTGGCATCCATTGAGAAGTACGGA
	GGCTACAACAAGGCGGCAGGTGCGTACTTCATGCTCGTCGAGTCCAAAGATAAGAAAGGC
	AAAACTATTAGAACAATCGAGTTCATCCCTCTATATTTGAAAAATAAGATCGAAAGTGAC
	GAAAGCATCGCCCTTAACTTCTTGGAGAAGGGCCGGGGCTTAAAGGAACCAAAGATTCTG
	CTCAAGAAGATCAAGATCGACACACTCTTCGATGTGGATGGTTTTAAGATGTGGCTGTCA
	GGCAGGACAGGGGATCGCTTGCTGTTCAAATGCGCAAATCAGTTGATTCTGGACGAAAAG
	ATCATTGTGACGATGAAGAAGATCGTTAAATTCATTCAGCGGAGACAGGAAAACAGAGAA
	CTGAAACTCTCCGATAAGGATGGAATTGACAATGAAGTCCTCATGGAGATTTACAATACC
	TTTGTGGACAAGCTTGAGAACACAGTCTATCGGATCCGACTGTCCGAACAGGCAAAGACT
	CTGATCGACAAACAGAAAGAATTCGAAAGACTAAGCTTAGAGGACAAAAGTTCAACTCTC
	TTTGAAATTCTCCACATCTTCCAATGTCAAAGTAGTGCAGCCAACTTGAAGATGATCGGG
	GGTCCCGGCAAGGCTGGAATCTTAGTCATGAACAACAACATCTCCAAATGTAACAAAATC
	TCCATCATAAACCAGTCTCCCACCGGCATTTTCGAGAACGAAATTGATTTACTCAAG

SEQ	ATGAAATCTTTCGATTCTTTCACCAACCTCTACTCCCTTAGCAAAACCCTTAAGTTTGAA
ID	ATGAGGCCGGTGGGGAATACACAGAAGATGCTTGACAATGCTGGCGTCTTTGAAAAGGAC
NO:	AAATTAATCCAGAAGAAGTATGGTAAAACAAAGCCATATTTTGACCGATTGCATCGGGAA
156	TTCATTGAAGAGGCTCTTACAGGAGTAGAATTGATCGGACTGGACGAGAACTTCCGTACC
	TTAGTAGACTGGCAGAAGGACAAGAAGAACAACGTGGCAATGAAGGCCTATGAGAACTCA
	CTCCAGCGCCTTAGAACCGAGATCGGAAAGATCTTTAATCTTAAGGCGGAAGATTGGGTA
	AAAAATAAGTACCCGATCCTGGGACTGAAAAACAAAAACACAGACATCCTGTTTGAAGAA
	GCCGTCTTTGGTATCTTGAAGGCCAGGTATGGAGAGGAGAAAGACACGTTTATAGAGGTA
	GAGGAGATTGATAAAACAGGCAAGAGTAAGATTAATCAGATCAGTATCTTTGATTCTTGG
	AAGGGGTTCACAGGCTACTTTAAGAAGTTTTTCGAAACCAGGAAAAATTTCTATAAGAAC
	GATGGCACCTCCACAGCTATCGCGACACGCATCATAGATCAGAATCTGAAACGGTTCATT
	GATAATCTGAGCATTGTTGAATCCGTGCGCCAGAAGGTCGACCTAGCTGAGACTGAGAAG
	TCTTTCTCTATATCACTCTCCCAGTTCTTCTCAATAGATTTTTATAATAAGTGCCTTCTG
	CAAGATGGCATAGACTACTATAACAAGATCATCGGCGGCGAAACTCTCAAAAACGGTGAA
	AAGCTCATTGGCCTGAATGAGCTCATCAACCAATATAGACAAAATAACAAGGATCAGAAA
	ATCCCATTCTTTAAGCTGCTAGATAAACAGATCCTATCAGAAAAAATCCTGTTCCTCGAC
	GAAATCAAAAACGACACCGAACTCATCGAGGCTCTCTCGCAGTTTGCCAAGACGGCTGAG
	GAGAAGACGAAGATTGTGAAAAAGCTGTTTGCAGACTTTGTGGAGAACAACTCTAAATAC
	GATTTGGCTCAGATTTATATCTCCCAGGAAGCATTTAACACAATCTCCAATAAGTGGACT
	AGCGAGACTGAAACCTTCGCCAAATACCTGTTCGAGGCCATGAAAAGCGGCAAGCTCGCC
	AAATACGAGAAGAAGGACAATTCCTATAAGTTTCCCGATTTCATCGCATTATCTCAGATG
	AAGTCCGCGCTACTTAGCATTAGCCTGGAAGGCCATTTTTGGAAGGAGAAATACTATAAG
	ATTTCCAAATTCCAAGAAAAGACCAATTGGGAGCAGTTCTTGGCTATTTTTCTATACGAG
	TTCAACTCTTTGTTCAGTGACAAGATCAACACTAAGGACGGTGAGACCAAACAAGTGGGG
	TACTACCTCTTCGCCAAAGATCTTCATAACCTGATACTGTCCGAACAGATCGACATACCC
	AAGGATTCAAAGGTGACCATCAAGGATTTTGCGGATTCGGTATTGACGATCTATCAGATG
	GCGAAGTATTTCGCTGTCGAGAAAAAGCGGGCATGGCTGGCCGAATACGAGTTGGACTCC
	TTCTATACTCAACCCGATACAGGGTACCTGCAGTTTTACGATAATGCATACGAGGATATA
	GTCCAGGTGTACAATAAACTCAGGAACTACCTCACTAAGAAACCATACTCCGAAGAAAAA
	TGGAAACTTAATTTTGAGAATAGTACACTGGCCAATGGATGGGACAAGAACAAGGAATCA
	GACAACTCCGCTGTAATTCTCCAGAAGGGTGGCAAGTATTATCTGGGACTGATAACAAAG
	GGCCATAACAAGATTTTCGATGACCGTTTTCAGGAGAAGTTTATAGTGGGCATAGAGGGT
	GGCAAGTATGAAAAAATAGTCTACAAGTTCTTTCCCGATCAGGCGAAGATGTTCCCCAAA
	GTATGCTTCAGTGCTAAAGGCCTCGAGTTTTTCCGGCCATCTGAAGAGATACTCCGCATC
	TATAATAACGCAGAGTTTAAAAAGGGAGAGACGTACTCAATCGACTCGATGCAGAAACTC
	ATTGACTTCTACAAAGATTGTCTCACAAAATACGAGGGCTGGGCTTGCTACACGTTTCGG
	CACTTGAAGCCAACCGAGGAATATCAAAACAACATCGGGGAGTTCTTCCGTGACGTCGCC
	GAAGACGGCTATAGAATTGACTTTCAGGGCATAAGTGATCAGTATATTCACGAGAAGAAT
	GAGAAAGGTGAGTTGCATCTTTTCGAAATCCACAATAAAGACTGGAATCTTGACAAGGCT
	CGCGATGGAAAATCAAAGACTACCCAGAAGAATCTTCATACACTTTACTTCGAGTCCCTC
	TTTTCCAACGACAACGTCGTACAGAATTTCCCAATAAAACTGAACGGCCAGGCCGAAATT
	TTTTACAGGCCCAAAACCGAAAAAGATAAACTGGAATCCAAGAAAGACAAGAAGGGAAAT
	AAGGTGATAGATCACAAAAGGTATTCCGAGAACAAGATTTTTTTCCACGTACCTCTTACC
	CTGAACAGAACGAAGAACGACTCTTATAGATTCAATGCCCAGATAAACAACTTTCTCGCA
	AACAACAAAGATATCAATATTATCGGCGTCGATAGAGGTGAGAAGCACTTGGTATATTAT
	TCTGTGATCACGCAAGCATCCGATATCTTGGAGTCCGGTTCTTTGAACGAACTGAATGGT
	GTCAACTACGCCGAGAAACTCGGTAAGAAAGCTGAGAATCGGGAGCAGGCTAGAAGGGAC
	TGGCAGGACGTTCAGGGTATCAAGGACCTGAAGAAGGGCTACATTTCTCAGGTGGTTCGA
	AAACTGGCTGATTTGGCCATTAAGCACAATGCAATCATCATTTTAGAAGATTTGAACATG
	CGGTTTAAACAAGTCAGGGGGGGGATAGAGAAATCAATTTACCAACAGCTGGAAAAAGCT
	CTGATTGATAAACTCTCTTTTTTGGTTGATAAGGGCGAAAAGAACCCCGAGCAAGCAGGA
	CATCTCCTTAAAGCCTATCAACTGAGCGCACCTTTCGAGACATTCCAGAAGATGGGAAAG
	CAAACCGGCATCATTTTCTATACCCAGGCTTCCTATACATCCAAGTCTGATCCAGTGACT
	GGGTGGAGACCCCATCTCTACCTCAAGTACTTTTCTGCCAAAAAAGCTAAGGACGACATT
	GCTAAGTTCACAAAAATCGAGTTCGTGAACGACAGGTTCGAGCTGACTTATGACATAAAA
	GATTTCCAGCAGGCCAAGGAGTACCCAAACAAGACAGTTTGGAAAGTGTGTTCCAATGTG
	GAGAGGTTTCGGTGGGACAAGAATCTGAATCAGAATAAAGGGGGATATACTCACTACACC
	AACATTACCGAGAACATCCAAGAGTTGTTCACCAAATACGGCATCGACATTACTAAAGAT
	CTGCTGACACAGATCTCCACCATCGATGAGAAGCAGAACACATCTTTCTTCCGGGATTTC
	ATCTTTTATTTTAACTTGATCTGTCAGATTAGAAATACCGACGACAGTGAGATAGCTAAA
	AAAAACGGGAAAGACGATTTCATTCTCTCTCCCGTGGAGCCGTTTTTTGACTCCCGCAAA
	GACAATGGCAATAAGCTTCCGGAAAACGGGGACGATAACGGCGCCTACAACATCGCTCGT
	AAGGGAATCGTTATCCTCAATAAAATAAGCCAGTATTCCGAGAAGAACGAGAATTGTGAA
	AAAATGAAGTGGGGGGACCTTTACGTCAGCAACATCGATTGGGATAACTTTGTGACACAA
	GCCAATGCGAGACACTAG

SEQ	ATGGAAAACTTCAAAAACCTCTACCCCATCAACAAGACCTTGAGGTTTGAGCTCCGGCCA
ID	TATGGGAAGACACTGGAGAACTTCAAAAAGTCCGGTCTGCTGGAAAAGGATGCTTTTAAG
NO:	GCTAACTCTAGGAGGTCTATGCAGGCCATTATCGATGAGAAATTCAAGGAGACCATAGAG
157	GAGCGTCTGAAATATACTGAGTTTTCCGAGTGTGACCTAGGAAATATGACCAGTAAGGAC
	AAAAAGATCACCGACAAGGCAGCGACAAACCTGAAGAAACAGGTGATTTTAAGCTTTGAT
	GATGAGATTTTCAATAACTACTTGAAGCCGGACAAAAACATCGACGCTCTGTTCAAGAAT
	GATCCAAGCAACCCGGTCATCTCTACTTTCAAGGGCTTCACCACATACTTTGTAAATTTC
	TTCGAAATACGGAAACACATCTTCAAGGGAGAGTCTTCCGGTAGCATGGCTTACAGAATA
	ATCGATGAGAACCTAACTACATATCTAAACAATATCGAGAAGATCAAGAAATTGCCTGAA
	GAACTGAAATCTCAGCTTGAGGGAATCGATCAAATTGACAAACTGAACAACTATAACGAG
	TTCATCACCCAGTCCGGCATTACTCATTATAACGAAATTATTGGAGGGATTTCGAAGTCT
	GAAAATGTCAAAATTCAAGGCATTAACGAAGGGATTAATCTTTACTGTCAAAAGAATAAA
	GTGAAGCTACCACGCTTAACTCCTCTGTATAAGATGATTCTCTCTGATCGGGTCTCTAAT
	TCCTTTGTGCTGGATACCATTGAAAATGATACCGAGTTAATTGAAATGATCTCTGATCTG
	ATAAATAAGACAGAGATAAGTCAGGATGTTATTATGTCCGACATCCAAAATATTTTCATC
	AAATATAAACAACTCGGCAACTTGCCGGGGATTAGCTACTCATCTATAGTGAATGCTATC
	TGTTCGGATTACGACAATAACTTTGGTGACGGCAAACGTAAAAAAAGCTATGAGAATGAT
	CGCAAAAAACACCTCGAGACTAACGTGTATAGCATTAACTATATCTCAGAGTTACTGACA
	GACACCGACGTCTCCAGCAACATAAAGATGCGGTACAAAGAGCTGGAGCAGAATTATCAG
	GTATGCAAGGAAAATTTCAACGCCACTAACTGGATGAACATCAAAAACATTAAGCAGTCT
	GAGAAAACCAATCTGATCAAGGACCTTCTTGACATCCTCAAGAGCATCCAGCGGTTTTAT
	GATTTGTTTGACATCGTGGATGAAGACAAAAATCCTAGTGCTGAGTTCTATACCTGGCTG
	TCTAAAAACGCGGAGAAACTGGACTTCGAGTTTAATTCAGTGTACAACAAGAGCAGGAAC
	TACCTCACGAGAAAGCAGTACTCCGATAAAAAGATTAAGTTGAACTTCGATAGTCCTACT
	CTCGCCAAGGGGTGGGATGCGAACAAAGAAATTGATAATAGCACAATTATCATGAGGAAG
	TTCAACAACGACCGGGGCGATTACGATTACTTCTTGGGGATCTGGAATAAGAGCACACCT
	GCCAACGAAAAGATCATCCCATTAGAGGATAATGGACTGTTTGAAAAAATGCAATATAAG
	CTGTATCCCGATCCTAGTAAAATGCTGCCAAAGCAATTCCTTTCTAAGATCTGGAAAGCT
	AAACATCCAACTACACCCGAGTTTGATAAGAAGTACAAAGAAGGTCGGCACAAGAAGGGG
	CCTGATTTTGAGAAAGAGTTTCTGCACGAGTTGATCGATTGCTTTAAGCATGGATTGGTA
	AACCACGACGAAAAATATCAGGATGTGTTCGGGTTCAATCTGCGCAACACGGAAGACTAC
	AACTCTTATACAGAGTTTCTGGAGGACGTCGAAAGGTGCAACTATAATCTTAGTTTCAAT
	AAAATCGCTGACACGTCTAACTTGATAAATGATGGGAAACTCTATGTTTTTCAGATCTGG
	AGCAAGGATTTCAGCATAGATAGCAAGGGAACAAAAAACTTGAACACAATATACTTTGAA
	TCCCTCTTCTCGGAGGAAAATATGATCGAGAAGATGTTCAAGCTCTCAGGGGAAGCCGAA
	ATATTCTATCGTCCAGCAAGTTTGAATTATTGTGAAGATATTATCAAGAAGGGACACCAC
	CACGCCGAACTGAAGGACAAATTCGACTATCCCATCATCAAGGACAAGCGATATAGCCAG
	GACAAATTTTTTTTTCATGTCCCCATGGTTATCAACTACAAAAGCGAGAAGTTAAACTCC
	AAATCACTTAACAATAGGACGAACGAAAATTTAGGCCAATTCACGCACATCATCGGTATC
	GACCGCGGAGAGCGACATCTCATCTACCTGACCGTGGTGGATGTGTCCACCGGTGAGATC
	GTTGAGCAAAAGCACCTGGATGAAATTATAAATACAGATACAAAAGGCGTCGAGCATAAA
	ACTCATTATCTCAATAAATTAGAAGAGAAGTCCAAGACGCGGGATAATGAAAGAAAGTCC
	TGGGAAGCAATCGAGACGATTAAGGAGCTGAAAGAAGGCTATATTAGCCACGTGATCAAT
	GAAATCCAGAAATTGCAGGAAAAGTATAACGCACTGATAGTGATGGAGAACCTCAATTAT
	GGGTTTAAGAACTCGCGTATCAAAGTGGAAAAGCAGGTCTACCAGAAATTCGAGACCGCC
	CTGATTAAAAAGTTTAATTACATCATTGACAAGAAAGATCCTGAAACCTACATTCATGGA
	TACCAACTGACGAATCCAATCACTACACTCGATAAAATTGGTAACCAGAGCGGTATTGTG
	TTGTACATTCCGGCTTGGAATACAAGCAAGATTGATCCAGTCACTGGTTTCGTTAACCTC
	CTGTATGCAGACGATTTGAAATACAAGAACCAGGAGCAGGCTAAAAGCTTTATCCAGAAA
	ATCGATAATATCTACTTCGAAAATGGTGAGTTTAAATTTGATATAGATTTCAGCAAATGG
	AACAACCGCTACTCAATTAGCAAGACGAAATGGACACTGACAAGCTACGGAACCCGGATA
	CAGACGTTCCGAAACCCCCAGAAAAATAACAAGTGGGACAGCGCCGAGTATGACCTGACC
	GAAGAGTTTAAATTAATCCTGAACATCGATGGTACTCTGAAATCTCAGGATGTGGAAACC
	TATAAGAAATTCATGTCTTTATTCAAGCTGATGTTGCAGCTGCGAAACTCCGTTACTGGA
	ACAGACATTGACTACATGATTAGCCCTGTGACAGATAAAACTGGAACCCACTTTGATTCA
	CGGGAGAATATCAAGAACCTGCCCGCCGATGCTGATGCGAACGGAGCTTACAACATTGCT
	AGGAAGGGCATCATGGCAATCGAGAATATTATGAACGGCATTAGCGACCCTCTGAAGATC
	AGTAATGAGGACTACCTGAAGTACATTCAGAACCAACAAGAGTAA

SEQ	ATGACCCAGTTTGAGGGTTTCACCAATCTTTATCAGGTGTCAAAAACACTCAGATTTGAG
ID	CTCATCCCACAGGGTAAAACTTTAAAGCATATTCAAGAGCAGGGCTTTATAGAGGAAGAC
NO:	AAAGCCAGAAACGACCATTATAAGGAACTAAAACCGATCATTGACCGCATCTACAAAACC
158	TATGCCGACCAATGCCTTCAGCTCGTCCAACTCGATTGGGAGAATCTGAGCGCCGCTATT
	GACAGCTACAGGAAGGAGAAGACCGAGGAGACTAGAAACGCCCTGATCGAGGAGCAGGCG
	ACCTATAGAAACGCTATTCACGATTATTTTATCGGCCGCACCGACAATTTGACAGATGCC
	ATCAACAAGCGGCACGCCGAAATTTATAAGGGGTTATTTAAGGCCGAGCTGTTCAATGGA
	AAAGTACTGAAACAGCTGGGCACCGTAACAACCACCGAACACGAGAATGCTCTGTTGAGG
	TCCTTCGACAAGTTTACTACCTACTTTAGCGGCTTCTACGAAAACCGTAAAAACGTGTTT
	TCCGCGGAGGATATTTCAACAGCCATTCCTCATAGGATCGTGCAGGATAATTTCCCCAAG
	TTTAAGGAGAACTGCCATATCTTTACCAGACTTATCACTGCTGTGCCAAGTTTACGAGAA
	CACTTCGAGAATGTTAAGAAGGCTATAGGCATATTCGTTTCCACCTCCATCGAAGAAGTA
	TTCAGTTTTCCATTCTACAATCAGTTACTCACGCAGACCCAGATAGATCTCTACAATCAG
	CTGCTCGGAGGCATTTCTAGAGAAGCAGGCACGGAAAAGATCAAGGGCTTAAATGAAGTA
	CTCAATCTTGCAATTCAGAAGAACGATGAGACAGCACACATTATTGCATCTCTCCCTCAC
	AGATTCATTCCCCTGTTCAAACAGATCCTGTCCGATCGCAACACACTAAGCTTTATACTT
	GAGGAGTTTAAGTCAGATGAGGAAGTGATCCAGAGCTTCTGTAAGTATAAGACTTTGCTC
	CGTAATGAAAACGTGCTTGAGACAGCAGAGGCTCTCTTTAACGAGTTGAATTCCATCGAC
	CTGACACACATTTTTATCAGCCATAAAAAGCTGGAAACGATTAGCTCTGCCTTGTGCGAC
	CACTGGGACACCCTGCGTAACGCCCTCTATGAAAGGCGCATTTCCGAGCTCACCGGGAAG
	ATCACAAAAAGTGCCAAGGAAAAAGTCCAGAGGTCCCTTAAACATGAAGACATCAACCTA
	CAAGAGATCATCTCTGCGGCTGGGAAAGAGCTGTCAGAAGCATTTAAACAGAAGACTTCC
	GAGATCCTGAGCCACGCACACGCCGCATTAGACCAGCCCCTGCCTACAACTCTTAAAAAA
	CAGGAGGAGAAGGAGATTTTAAAGAGCCAGCTGGACTCATTACTCGGCCTGTATCATCTC
	CTGGACTGGTTCGCCGTGGACGAATCCAACGAGGTGGACCCAGAATTTAGCGCCAGGCTG
	ACAGGAATTAAACTGGAAATGGAGCCAAGTTTGAGCTTTTACAACAAGGCTCGGAACTAT
	GCCACTAAAAAGCCCTACAGCGTGGAAAAGTTCAAGCTGAATTTTCAGATGCCGACCCTG
	GCTTCCGGGTGGGATGTTAATAAGGAAAAGAATAATGGGGCTATACTGTTCGTCAAAAAT
	GGTCTCTACTACCTGGGAATCATGCCCAAACAGAAGGGCAGGTACAAAGCCCTTTCGTTT
	GAGCCGACCGAAAAAACCAGCGAAGGCTTTGATAAGATGTATTACGACTATTTCCCAGAT
	GCAGCCAAGATGATCCCAAAATGTAGCACTCAGTTGAAGGCGGTAACCGCTCACTTTCAG
	ACACACACCACTCCTATCTTGCTCTCCAACAACTTTATTGAGCCGCTGGAGATCACGAAG
	GAAATCTACGACCTTAACAACCCAGAGAAGGAACCCAAGAAATTCCAAACAGCTTATGCT
	AAGAAGACTGGGGATCAAAAGGGCTATCGAGAGGCTTTGTGTAAGTGGATTGACTTTACA
	CGGGATTTCCTGAGTAAGTATACCAAGACCACATCTATTGACCTGTCCTCACTGAGACCT
	TCCTCACAATATAAGGATCTCGGAGAGTATTATGCCGAACTCAACCCTCTACTCTATCAC
	ATCTCTTTCCAGAGGATCGCCGAAAAGGAAATTATGGACGCCGTCGAGACAGGCAAGCTG
	TACCTCTTCCAGATTTACAACAAGGATTTCGCAAAGGGCCACCACGGAAAACCCAATTTG
	CACACTTTGTACTGGACAGGGCTCTTCTCTCCCGAAAATTTGGCCAAAACTTCAATAAAA
	CTGAACGGGCAAGCCGAGCTGTTCTATCGGCCCAAGTCACGTATGAAGCGGATGGCCCAC
	CGGCTGGGCGAGAAGATGCTCAACAAGAAACTGAAGGATCAGAAGACGCCCATACCAGAC
	ACTCTTTACCAAGAGCTGTATGACTACGTGAATCACAGACTGAGTCACGACCTGTCTGAT
	GAAGCCCGGGCTCTTCTTCCAAATGTGATTACCAAAGAAGTTTCCCACGAAATTATCAAG
	GACCGGCGCTTCACCTCTGACAAATTCTTTTTCCACGTCCCAATCACCCTCAACTACCAG
	GCAGCCAATTCCCCTTCAAAGTTTAACCAGCGTGTGAATGCCTACCTGAAAGAGCATCCG
	GAGACCCCCATCATAGGGATAGACAGAGGAGAGCGGAATCTTATCTACATTACTGTGATT
	GACAGCACAGGTAAGATCTTGGAGCAGAGATCTTTAAATACAATCCAGCAGTTTGACTAC
	CAGAAGAAACTGGATAACCGAGAGAAGGAAAGGGTTGCTGCAAGACAGGCCTGGTCAGTG
	GTCGGCACCATCAAAGACCTGAAGCAGGGCTACTTATCCCAAGTAATTCACGAAATTGTC
	GATCTTATGATTCATTATCAAGCCGTTGTTGTGCTGGAGAACCTGAATTTTGGCTTCAAA
	AGCAAACGAACAGGTATCGCCGAGAAAGCCGTGTATCAGCAGTTCGAAAAGATGCTCATA
	GACAAGCTGAACTGCTTAGTGCTGAAGGATTATCCTGCTGAGAAGGTCGGCGGCGTACTT
	AACCCATACCAGCTGACCGATCAGTTCACTAGTTTCGCCAAGATGGGAACGCAAAGTGGC
	TTCCTTTTCTACGTGCCCGCTCCCTACACGAGTAAGATCGACCCTCTGACCGGCTTCGTC
	GACCCATTCGTCTGGAAGACCATCAAGAATCACGAATCACGGAAACACTTCTTAGAGGGG
	TTTGACTTCCTGCACTACGACGTGAAGACAGGGGACTTCATCTTACACTTTAAGATGAAT
	CGAAACCTCTCCTTCCAGCGGGGCCTGCCTGGTTTCATGCCCGCATGGGACATCGTGTTT
	GAGAAAAACGAGACACAGTTTGACGCTAAGGGAACCCCCTTTATTGCGGGGAAGCGGATT
	GTCCCAGTCATCGAAAACCATCGGTTCACCGGGCGATACCGGGATCTGTACCCGGCCAAC
	GAGCTCATCGCGCTGCTGGAGGAGAAGGGTATTGTGTTTAGGGATGGATCCAACATTCTG
	CCTAAGTTGCTGGAAAATGATGATTCGCACGCCATTGATACCATGGTTGCACTGATTAGA
	TCCGTACTGCAGATGAGGAATAGCAATGCTGCAACCGGGGAGGATTATATTAATTCCCCA
	GTGCGAGATCTGAATGGTGTCTGTTTTGACTCGCGCTTTCAGAATCCAGAATGGCCAATG
	GATGCAGACGCTAACGGGGCGTACCACATTGCTCTGAAAGGCCAGCTACTCCTGAACCAC
	CTCAAGGAGAGCAAAGATCTGAAGCTGCAGAACGGCATTTCCAACCAAGACTGGCTCGCC
	TACATACAAGAACTGCGCAATTAA

SEQ	ATGGCTGTCAAATCCATCAAGGTTAAATTACGGCTTGATGACATGCCCGAGATCCGCGCC
ID	GGGCTCTGGAAACTCCATAAAGAAGTGAATGCTGGCGTTAGATACTACACAGAATGGCTC
NO:	TCCCTGCTGCGCCAGGAAAATTTGTACCGCCGGTCACCTAATGGAGATGGAGAGCAGGAA
159	TGCGATAAAACAGCAGAAGAGTGCAAAGCCGAATTGCTGGAGCGACTGCGGGCACGGCAG
	GTTGAGAATGGACACCGAGGTCCGGCGGGATCGGACGACGAGCTGCTCCAGCTCGCCAGA
	CAATTATATGAACTGCTGGTGCCTCAGGCTATTGGGGCAAAGGGTGACGCACAGCAGATT
	GCTAGAAAATTTCTGTCTCCCCTCGCCGACAAAGACGCTGTCGGCGGCCTTGGGATAGCC
	AAAGCCGGCAACAAACCCCGATGGGTGCGCATGAGGGAGGCTGGTGAGCCTGGCTGGGAG
	GAAGAAAAGGAAAAGGCCGAAACCAGAAAGTCCGCCGACAGGACCGCGGACGTACTCCGA
	GCATTGGCCGATTTTGGGCTGAAGCCCTTAATGCGAGTCTACACCGATAGTGAAATGTCT
	AGCGTGGAGTGGAAGCCATTACGCAAAGGGCAGGCAGTGCGGACGTGGGACCGTGACATG
	TTCCAGCAAGCCATCGAGCGAATGATGAGCTGGGAGAGCTGGAACCAGAGAGTGGGGCAG
	GAGTATGCCAAGCTGGTCGAGCAGAAAAACCGGTTTGAGCAAAAAAATTTTGTAGGTCAG
	GAACACCTGGTGCATCTCGTTAACCAGCTCCAGCAAGATATGAAGGAAGCTTCGCCTGGA
	TTAGAGAGCAAAGAGCAGACTGCACACTATGTAACCGGAAGAGCACTGAGGGGCAGTGAC
	AAAGTGTTCGAAAAATGGGGAAAACTGGCTCCCGATGCCCCCTTTGACCTGTACGACGCA
	GAAATAAAAAACGTGCAGCGGCGAAACACCAGGCGATTTGGTAGCCATGATCTGTTCGCC
	AAATTGGCAGAGCCGGAATATCAGGCTCTTTGGCGAGAAGACGCATCATTTCTCACTAGG
	TACGCGGTCTATAACTCCATTTTGAGGAAATTGAACCACGCAAAAATGTTTGCCACCTTC
	ACGTTGCCTGACGCCACCGCTCATCCCATTTGGACACGGTTTGATAAGCTGGGCGGCAAT
	CTGCATCAGTATACATTCCTGTTTAACGAGTTTGGAGAGCGAAGACATGCGATACGATTC
	CACAAGCTACTGAAGGTCGAAAATGGCGTGGCACGTGAGGTGGACGATGTCACCGTGCCC
	ATCAGCATGAGCGAACAGCTGGATAATTTGTTGCCGCGGGACCCAAATGAACCTATAGCC
	CTTTATTTTAGGGACTACGGGGCGGAGCAACATTTCACTGGGGAGTTTGGCGGCGCAAAA
	ATTCAGTGCCGACGCGACCAGCTCGCCCACATGCATAGAAGACGCGGGGCCCGGGACGTA
	TACCTTAACGTCTCTGTGAGGGTGCAGTCCCAGTCAGAGGCAAGAGGGGAACGCAGACCA
	CCTTACGCAGCAGTATTCAGGCTGGTAGGCGATAACCACCGGGCGTTTGTACACTTTGAT
	AAACTTTCTGACTACCTGGCCGAACACCCGGATGACGGCAAATTAGGATCGGAGGGGCTG
	CTTAGCGGCCTGCGTGTGATGAGCGTCGATCTGGGGCTACGGACCTCTGCTTCCATCTCT
	GTGTTCCGTGTGGCCCGAAAGGACGAGTTGAAACCTAATTCGAAGGGCCGTGTACCATTC
	TTTTTCCCTATTAAGGGAAATGATAATCTCGTCGCGGTGCACGAGCGTTCCCAACTGCTG
	AAACTGCCTGGCGAGACCGAGTCCAAAGATCTCAGAGCAATCCGGGAGGAGCGACAACGT
	ACACTTAGGCAACTCCGCACCCAGCTGGCCTATCTGCGCTTGCTGGTGCGGTGCGGCTCC
	GAGGATGTAGGGAGAAGAGAGCGAAGCTGGGCAAAGCTGATAGAGCAACCAGTTGACGCC
	GCGAATCACATGACCCCCGACTGGCGCGAAGCGTTTGAAAATGAGCTGCAGAAGTTGAAA
	TCTCTGCATGGGATTTGCTCAGATAAGGAGTGGATGGACGCCGTATACGAGTCTGTTCGC
	CGGGTATGGCGGCACATGGGGAAGCAGGTGAGAGATTGGAGAAAGGACGTTCGCTCTGGG
	GAACGGCCGAAAATTCGGGGATACGCAAAGGATGTCGTGGGCGGCAATAGCATTGAGCAG
	ATCGAGTACCTGGAAAGGCAATACAAATTTCTGAAATCTTGGTCTTTCTTTGGGAAGGTA
	AGCGGACAAGTTATCAGAGCCGAAAAGGGATCTCGCTTTGCTATCACATTGAGGGAACAC
	ATTGATCACGCCAAAGAAGACAGGTTGAAAAAGTTGGCTGATCGCATTATCATGGAAGCA
	CTCGGTTACGTCTACGCCCTTGATGAGCGCGGTAAAGGGAAGTGGGTAGCCAAGTATCCC
	CCATGTCAGCTGATCCTGCTCGAGGAACTTTCTGAGTATCAGTTCAATAACGACCGTCCT
	CCCTCCGAAAATAATCAGCTCATGCAATGGTCCCACCGGGGTGTGTTCCAAGAACTGATC
	AATCAGGCTCAGGTGCACGACCTCCTCGTAGGCACTATGTATGCAGCCTTTAGCTCCCGT
	TTTGACGCGCGCACAGGCGCCCCTGGAATACGATGTAGGCGAGTTCCCGCACGGTGCACT
	CAAGAACATAACCCGGAGCCTTTCCCATGGTGGCTCAATAAGTTTGTTGTGGAGCATACC
	CTCGACGCTTGCCCATTGAGGGCGGATGACTTGATTCCCACAGGCGAGGGGGAGATCTTC
	GTGAGCCCATTTTCTGCCGAAGAAGGGGATTTCCACCAAATACATGCCGACTTGAATGCT
	GCCCAAAATCTGCAGCAAAGGCTGTGGTCAGACTTCGACATCTCGCAAATCAGACTGCGG
	TGTGACTGGGGCGAAGTAGACGGCGAGCTGGTGCTGATACCTAGACTGACGGGTAAGCGT
	ACCGCCGATAGCTATAGTAATAAGGTTTTTTATACGAATACGGGGGTGACATATTACGAG
	CGTGAGAGAGGCAAGAAGCGTCGGAAGGTGTTCGCGCAGGAGAAGCTGAGCGAAGAGGAG
	GCGGAGCTACTGGTAGAGGCAGATGAGGCAAGAGAAAAGTCCGTCGTCCTGATGCGGGAT
	CCTAGCGGGATTATTAACAGAGGTAATTGGACACGGCAGAAAGAATTCTGGAGCATGGTG
	AATCAAAGAATCGAGGGTTACCTGGTGAAGCAAATTCGAAGCCGGGTGCCCCTTCAAGAC
	AGCGCATGTGAAAACACTGGGGACATCTAG

SEQ	ATGGCTACTCGGTCCTTCATCCTGAAAATCGAGCCAAATGAAGAGGTGAAAAAGGGCCTG
ID	TGGAAGACCCATGAGGTACTTAACCACGGCATAGCATACTATATGAATATCCTAAAACTT
NO:	ATACGGCAGGAGGCTATCTACGAGCATCACGAGCAAGATCCTAAAAATCCAAAGAAGGTT
160	AGTAAGGCTGAAATCCAGGCTGAATTGTGGGACTTCGTGCTGAAGATGCAGAAATGCAAC
	AGTTTCACGCATGAAGTTGATAAGGACGTCGTGTTTAATATACTCCGGGAGCTGTACGAA
	GAACTGGTACCAAGCTCTGTGGAAAAGAAAGGAGAGGCCAACCAGCTAAGTAATAAGTTC
	CTCTATCCTCTCGTGGACCCCAATTCACAGAGCGGCAAAGGTACCGCATCTTCTGGGAGG
	AAACCACGCTGGTACAACTTGAAGATCGCTGGCGATCCCAGCTGGGAGGAGGAAAAGAAG
	AAATGGGAAGAGGATAAAAAGAAAGACCCCCTGGCCAAAATCTTAGGCAAGCTCGCCGAG
	TACGGTCTGATTCCACTTTTCATCCCGTTCACAGATAGCAATGAGCCGATCGTCAAGGAG
	ATTAAGTGGATGGAAAAGAGCCGCAATCAGAGTGTGCGGAGGCTGGACAAAGACATGTTT
	ATTCAGGCCCTGGAACGCTTCCTTAGCTGGGAAAGCTGGAACCTGAAGGTTAAGGAAGAG
	TACGAAAAAGTCGAGAAGGAGCATAAGACTTTGGAGGAGCGCATCAAAGAAGACATCCAG
	GCCTTTAAGTCTCTAGAACAGTATGAGAAAGAACGGCAGGAACAGCTGCTGCGTGATACA
	CTGAACACAAACGAATATCGCCTGAGCAAGAGGGGACTCAGAGGCTGGAGAGAAATCATT
	CAAAAGTGGCTCAAAATGGATGAAAATGAGCCGTCTGAAAAATACCTTGAAGTTTTCAAG
	GACTACCAGCGGAAGCACCCTAGAGAAGCCGGCGACTATAGTGTTTACGAATTCTTGAGC
	AAGAAGGAGAATCATTTTATATGGAGGAATCACCCGGAGTACCCATATCTGTACGCAACC
	TTCTGCGAAATCGACAAGAAAAAAAAAGACGCCAAGCAACAGGCTACATTTACTCTGGCC
	GACCCTATCAATCACCCTCTATGGGTCCGGTTTGAGGAGCGCTCCGGAAGCAATCTGAAT
	AAATATCGTATTCTGACTGAACAGTTACACACAGAGAAGCTCAAGAAGAAACTTACGGTG
	CAGCTGGACCGCCTGATATACCCAACAGAGTCCGGAGGATGGGAAGAGAAAGGAAAGGTT
	GACATCGTACTGCTTCCATCTCGTCAGTTTTACAACCAGATATTCCTGGACATCGAGGAG
	AAGGGGAAACACGCCTTCACATACAAGGACGAGTCCATAAAGTTCCCACTGAAGGGTACT
	TTAGGCGGTGCTAGGGTGCAGTTCGACCGCGATCACCTGAGACGGTACCCCCACAAGGTG
	GAGAGCGGGAACGTGGGACGAATCTACTTTAATATGACAGTGAACATTGAACCCACAGAG
	AGTCCAGTTAGTAAATCCCTGAAAATTCACCGTGACGACTTTCCGAAATTTGTGAATTTC
	AAGCCAAAGGAGCTTACGGAGTGGATCAAGGATTCAAAGGGAAAGAAGCTGAAATCTGGT
	ATCGAATCTCTCGAGATCGGTCTCCGTGTCATGAGCATCGATCTGGGACAGCGCCAGGCA
	GCTGCCGCCAGTATATTCGAGGTGGTAGACCAAAAGCCTGACATCGAGGGAAAGCTCTTC
	TTCCCAATCAAAGGCACAGAGCTGTATGCGGTGCACCGGGCGTCCTTTAATATAAAGCTG
	CCCGGTGAAACCCTGGTGAAGTCACGGGAGGTGCTTAGAAAAGCGCGAGAGGATAACCTC
	AAACTGATGAACCAAAAACTGAACTTTCTGAGGAACGTCCTGCACTTTCAGCAGTTCGAA
	GATATTACCGAACGCGAAAAGAGAGTAACCAAGTGGATATCTCGTCAAGAGAACAGCGAC
	GTCCCGTTAGTCTATCAGGACGAACTCATCCAAATACGGGAGTTGATGTATAAGCCCTAC
	AAGGATTGGGTCGCCTTTCTTAAGCAGCTTCACAAACGCCTAGAGGTCGAAATAGGTAAA
	GAGGTGAAACATTGGCGGAAGTCGCTCAGCGACGGGAGGAAGGGACTTTATGGCATCTCT
	TTGAAGAACATTGACGAAATCGATAGAACCAGAAAATTTTTGTTGAGATGGTCCCTCCGA
	CCCACCGAGCCTGGAGAGGTGAGGCGGTTAGAACCAGGACAGAGGTTCGCTATCGATCAG
	CTGAATCACCTCAATGCTCTGAAGGAGGACCGCCTCAAGAAAATGGCCAATACAATCATA
	ATGCACGCCCTTGGCTACTGCTACGACGTCCGAAAGAAGAAGTGGCAGGCCAAGAATCCC
	GCCTGTCAAATTATCCTTTTTGAGGATCTTAGCAATTACAACCCCTATGAAGAGCGGTCC
	AGATTCGAAAATAGTAAGCTCATGAAGTGGAGCCGCAGGGAGATCCCGCGCCAAGTGGCC
	CTTCAGGGGGAAATTTATGGGCTGCAGGTAGGCGAGGTCGGGGCCCAATTCTCCTCGCGC
	TTTCATGCGAAAACTGGAAGTCCTGGAATCCGGTGCTCAGTGGTGACAAAGGAGAAGTTG
	CAAGACAATCGGTTTTTTAAAAACTTACAGCGGGAGGGAAGGCTGACCCTGGATAAGATA
	GCCGTACTTAAGGAAGGAGATCTGTACCCTGACAAAGGCGGTGAAAAGTTCATTAGCTTG
	AGCAAGGACCGAAAACTTGTGACCACCCACGCTGACATCAATGCGGCACAGAACCTGCAG
	AAGAGATTTTGGACTCGCACCCACGGATTCTACAAAGTTTACTGCAAAGCATATCAAGTA
	GACGGACAGACCGTATACATCCCCGAGTCCAAAGATCAGAAGCAGAAAATTATTGAAGAG
	TTTGGGGAAGGGTACTTTATCCTGAAGGATGGTGTCTACGAATGGGGCAACGCTGGTAAA
	CTTAAAATTAAGAAGGGCAGCTCTAAACAGTCCTCCAGCGAGTTAGTTGATTCTGATATT
	CTGAAAGACAGTTTCGACCTGGCCAGCGAACTTAAAGGGGAAAAATTAATGCTGTACCGG
	GACCCCAGCGGAAACGTCTTTCCATCCGATAAGTGGATGGCCGCTGGAGTGTTCTTTGGC
	AAGTTAGAGAGGATTCTCATAAGTAAGCTGACCAACCAATACTCAATCTCCACAATCGAG
	GATGACTCATCCAAGCAGTCTATGTGA

SEQ	ATGCCTACACGCACTATCAACCTGAAACTGGTTCTTGGCAAGAATCCAGAGAATGCTACC
ID	CTTCGTCGGGCACTATTTTCAACGCATAGACTGGTGAATCAGGCTACCAAACGGATTGAA
NO:	GAGTTCCTCTTGCTTTGTCGGGGGGAAGCATATAGGACGGTGGATAATGAGGGGAAAGAG
161	GCTGAAATTCCGAGACACGCCGTGCAGGAGGAAGCTCTTGCGTTTGCAAAGGCCGCTCAA
	CGGCACAATGGTTGCATCTCTACTTATGAAGACCAGGAAATCCTGGATGTGCTCCGGCAA
	CTGTATGAAAGGCTGGTGCCTTCTGTGAATGAAAATAATGAAGCAGGGGACGCTCAAGCC
	GCAAACGCGTGGGTGTCGCCACTGATGTCCGCCGAGTCCGAGGGAGGGCTCAGCGTTTAC
	GACAAGGTGCTGGACCCACCCCCAGTGTGGATGAAACTCAAAGAGGAAAAAGCTCCGGGC
	TGGGAGGCTGCTTCCCAGATCTGGATCCAGTCCGACGAAGGGCAGTCCCTTCTTAACAAG
	CCTGGTTCGCCCCCGCGGTGGATTAGGAAACTGAGGTCAGGCCAGCCTTGGCAGGACGAT
	TTTGTTAGCGACCAGAAAAAGAAGCAGGACGAGCTGACAAAGGGGAATGCGCCACTGATC
	AAACAATTAAAGGAAATGGGCTTATTGCCTCTTGTGAATCCCTTTTTTAGACATCTGCTT
	GACCCGGAGGGGAAGGGGGTGTCACCTTGGGACAGACTCGCTGTTAGGGCCGCTGTCGCT
	CATTTCATATCATGGGAATCATGGAACCACCGGACACGCGCCGAATACAATAGTTTGAAG
	CTGCGGAGGGATGAGTTCGAAGCAGCTTCCGACGAATTCAAGGACGACTTCACGCTGCTT
	CGGCAGTACGAGGCTAAGAGGCACTCCACACTGAAGAGTATAGCTTTAGCCGATGATTCA
	AACCCTTATAGGATCGGCGTACGCTCCCTCCGCGCTTGGAACCGCGTCCGCGAGGAGTGG
	ATCGACAAGGGAGCGACCGAGGAGCAGCGGGTCACCATTCTCAGCAAGTTGCAGACCCAA
	CTAAGGGGCAAATTTGGAGATCCTGACTTGTTCAACTGGCTGGCGCAGGACCGGCACGTG
	CACCTCTGGAGCCCTAGAGATAGTGTTACCCCACTGGTTAGGATCAACGCTGTTGACAAA
	GTATTGCGACGGAGAAAACCGTACGCCTTGATGACTTTTGCCCACCCAAGATTCCACCCT
	CGGTGGATACTTTACGAAGCCCCAGGGGGCAGCAATCTCCGCCAGTATGCACTGGATTGT
	ACCGAAAATGCTCTGCACATTACACTGCCTCTGCTGGTTGACGATGCACATGGCACATGG
	ATTGAGAAAAAAATTAGGGTTCCTCTTGCCCCCAGCGGCCAGATTCAGGACCTGACACTA
	GAAAAGCTCGAGAAGAAGAAAAATCGTCTCTACTACCGTTCTGGGTTCCAGCAGTTTGCC
	GGCCTGGCCGGAGGTGCCGAGGTGCTTTTCCATCGACCATACATGGAGCACGATGAGAGG
	AGCGAGGAGAGCTTATTAGAACGCCCTGGTGCTGTTTGGTTCAAACTCACCTTGGACGTG
	GCAACCCAGGCCCCTCCAAACTGGTTGGACGGAAAGGGCCGCGTCCGAACGCCCCCCGAG
	GTTCACCACTTCAAGACAGCCCTCAGTAACAAGTCTAAGCACACACGGACCCTCCAGCCC
	GGACTCAGAGTGTTATCCGTGGATCTGGGAATGCGCACCTTCGCCTCTTGCTCCGTATTT
	GAGCTGATCGAGGGCAAACCAGAGACTGGCAGAGCGTTCCCTGTGGCCGACGAACGTTCC
	ATGGATTCACCAAACAAGCTGTGGGCCAAGCACGAAAGATCCTTTAAACTCACGCTCCCC
	GGCGAAACCCCCAGTCGGAAAGAAGAGGAGGAACGGAGCATTGCAAGAGCCGAAATCTAT
	GCGTTGAAAAGAGATATTCAGAGATTAAAAAGTCTTCTGCGCCTGGGGGAAGAGGATAAC
	GATAATAGACGCGATGCACTTCTTGAGCAATTTTTCAAGGGCTGGGGCGAGGAAGACGTG
	GTTCCAGGTCAGGCCTTTCCCCGGAGTCTGTTCCAGGGGCTGGGGGCCGCCCCATTCAGA
	TCCACCCCTGAGTTGTGGAGACAACACTGTCAAACCTATTATGATAAAGCAGAGGCGTGC
	CTGGCTAAACACATCAGCGATTGGCGCAAGAGAACCAGGCCTAGGCCTACCTCACGTGAG
	ATGTGGTACAAGACACGCTCTTATCACGGCGGAAAGTCAATCTGGATGCTGGAATACCTC
	GACGCTGTGAGGAAACTGCTCTTATCCTGGAGCCTCAGAGGCCGGACCTACGGGGCTATC
	AACAGACAGGACACAGCAAGGTTCGGGAGCTTAGCCAGCCGGCTCCTTCACCACATTAAC
	TCACTCAAAGAGGATCGAATAAAGACCGGAGCCGACTCGATCGTGCAGGCAGCCCGAGGG
	TACATCCCCCTGCCTCATGGGAAGGGCTGGGAGCAGCGATATGAACCCTGCCAGCTGATC
	TTGTTTGAGGACCTTGCCCGTTATAGATTTCGCGTTGATAGACCTCGCCGTGAGAATTCT
	CAGCTGATGCAGTGGAACCACAGAGCGATCGTGGCTGAGACCACTATGCAGGCCGAGCTG
	TATGGACAGATCGTGGAGAACACCGCCGCAGGGTTCAGTTCTCGGTTTCATGCTGCCACC
	GGAGCTCCCGGCGTCCGGTGCCGCTTCCTCTTAGAGCGTGATTTTGACAATGACCTCCCA
	AAGCCCTATCTGCTGAGGGAACTGAGCTGGATGCTGGGGAACACAAAAGTAGAATCGGAG
	GAGGAGAAGCTACGGCTCCTCTCCGAAAAGATACGTCCAGGCTCTCTGGTACCATGGGAC
	GGAGGAGAGCAGTTCGCGACACTGCATCCTAAGAGACAGACGTTATGTGTGATTCACGCC
	GATATGAACGCCGCTCAGAATCTGCAGCGAAGATTCTTTGGCCGCTGCGGCGAAGCCTTC
	AGGCTGGTATGTCAGCCCCACGGGGATGATGTGCTGCGGCTGGCCTCAACCCCTGGGGCT
	AGACTCTTGGGGGCACTCCAGCAGCTGGAAAATGGCCAAGGGGCTTTCGAACTCGTTCGG
	GACATGGGCAGCACAAGCCAGATGAACAGATTCGTCATGAAGAGCCTGGGAAAGAAAAAG
	ATCAAACCCTTACAGGACAATAATGGCGACGACGAACTGGAGGACGTGTTGTCCGTGCTG
	CCAGAGGAAGACGACACAGGCCGCATCACTGTCTTCCGCGACTCAAGTGGGATATTCTTT
	CCTTGCAACGTGTGGATTCCGGCCAAACAGTTCTGGCCTGCCGTCAGAGCCATGATTTGG
	AAAGTGATGGCTAGTCATTCATTGGGATGA

SEQ	ATGACAAAGCTGAGGCACAGACAAAAGAAGCTTACACACGACTGGGCAGGGAGCAAGAAA
ID	CGTGAGGTCCTTGGGTCAAATGGAAAACTGCAGAACCCCTTGCTCATGCCTGTAAAGAAG
NO:	GGGCAGGTAACAGAATTTAGAAAAGCATTCTCCGCGTACGCTCGGGCAACTAAGGGGGAA
162	ATGACCGATGGACGGAAGAACATGTTCACCCATTCTTTCGAGCCATTCAAAACAAAGCCG
	TCATTGCACCAATGCGAGCTGGCCGATAAGGCTTACCAGTCTTTGCATAGTTACCTCCCC
	GGTTCCCTGGCCCATTTCTTGCTTTCCGCACACGCACTGGGCTTTCGTATTTTCTCTAAA
	TCTGGGGAGGCAACTGCCTTCCAGGCCAGCTCAAAAATCGAGGCCTATGAGTCCAAGCTC
	GCTTCGGAGCTAGCCTGTGTCGATTTGAGTATCCAGAATTTGACGATTAGTACTCTTTTC
	AACGCTCTCACAACTTCAGTTCGGGGCAAGGGGGAGGAAACTTCAGCAGATCCCCTTATC
	GCACGGTTCTACACTCTCCTGACGGGCAAGCCCCTGAGCCGAGACACACAGGGCCCAGAA
	CGGGACTTGGCAGAGGTCATCTCCAGAAAGATCGCCTCGTCCTTCGGCACATGGAAGGAA
	ATGACTGCCAACCCTCTGCAGAGCCTCCAGTTCTTCGAAGAAGAGCTTCATGCACTAGAT
	GCCAACGTGTCTTTATCTCCAGCTTTTGATGTGTTAATCAAGATGAATGATCTCCAAGGT
	GATCTGAAGAACCGTACTATAGTGTTCGACCCAGATGCACCCGTGTTCGAGTACAACGCT
	GAGGATCCAGCCGATATCATCATAAAGCTGACAGCTCGGTATGCGAAGGAGGCCGTCATC
	AAGAATCAGAACGTGGGCAATTATGTGAAAAACGCCATTACCACCACTAATGCCAATGGG
	CTGGGGTGGCTCCTCAATAAAGGGCTTTCACTACTGCCAGTTTCTACTGACGATGAGCTG
	CTCGAATTCATTGGGGTGGAGAGAAGCCATCCCAGCTGTCACGCGCTGATAGAGCTGATT
	GCCCAGCTAGAGGCGCCGGAACTGTTTGAGAAGAATGTGTTTAGTGACACCCGTTCCGAG
	GTTCAGGGTATGATCGACAGTGCAGTGTCGAACCACATTGCTCGGCTGTCCAGCAGCCGA
	AACTCCCTGAGCATGGACAGCGAGGAATTGGAACGCTTGATTAAATCTTTCCAGATTCAT
	ACTCCCCATTGTTCTCTGTTCATAGGCGCTCAGTCCTTATCTCAGCAGCTGGAGAGCTTA
	CCTGAGGCGCTGCAGTCCGGAGTGAACAGCGCTGATATCTTATTAGGCAGCACACAGTAT
	ATGCTGACCAACTCTCTCGTTGAAGAGTCAATTGCAACATATCAAAGGACATTAAATAGG
	ATCAATTACCTGAGTGGGGTGGCTGGGCAGATTAACGGTGCTATCAAAAGAAAGGCAATC
	GACGGCGAAAAAATACACCTGCCTGCCGCCTGGAGTGAGCTCATCTCCTTACCTTTCATT
	GGACAGCCGGTGATTGATGTGGAGAGCGACCTGGCACACTTAAAAAACCAGTACCAGACC
	CTGTCCAATGAATTTGACACCCTCATTTCGGCCCTGCAGAAGAACTTCGATTTGAATTTC
	AACAAAGCACTCCTTAACCGCACGCAGCATTTCGAGGCAATGTGCCGGAGCACAAAAAAA
	AATGCTTTATCTAAGCCCGAGATCGTGTCCTACAGAGATCTGCTGGCGCGGCTGACCAGT
	TGCCTTTATCGAGGCTCGCTGGTTCTCAGAAGGGCGGGAATCGAAGTTCTGAAAAAGCAC
	AAAATCTTTGAGTCGAATAGTGAGCTGAGAGAACACGTCCACGAGCGAAAGCACTTCGTG
	TTCGTTAGTCCATTGGACAGAAAGGCAAAAAAACTGTTGCGCCTGACCGATTCCCGCCCT
	GACTTGCTCCATGTGATCGATGAGATCCTGCAACATGACAATCTGGAGAATAAGGACAGA
	GAGTCCCTTTGGCTGGTCCGGTCTGGGTACCTCCTTGCTGGTCTGCCGGACCAGCTGAGT
	TCTTCGTTTATCAATCTCCCCATAATCACGCAAAAGGGCGATCGCCGGCTGATTGACCTG
	ATTCAGTATGACCAGATCAATCGCGATGCTTTCGTAATGTTGGTGACAAGTGCTTTCAAA
	AGCAATCTCTCTGGGTTGCAGTACCGCGCTAACAAGCAGTCTTTCGTGGTCACCCGCACC
	CTGTCTCCTTACCTGGGTAGTAAGCTCGTATACGTCCCTAAAGACAAAGATTGGCTGGTC
	CCATCCCAGATGTTTGAGGGAAGATTCGCCGATATTCTGCAGAGTGACTACATGGTCTGG
	AAGGATGCCGGACGCCTGTGCGTGATCGACACTGCCAAACATCTCTCTAACATTAAAAAA
	AGCGTGTTTAGTAGCGAAGAAGTCCTTGCTTTTCTTCGAGAGCTGCCTCACCGGACCTTC
	ATCCAGACCGAGGTACGGGGGTTAGGAGTGAACGTCGATGGAATCGCATTTAATAACGGG
	GATATCCCGAGCTTGAAGACATTCTCGAATTGTGTGCAGGTGAAGGTGAGTAGGACTAAT
	ACTAGTCTCGTGCAGACTCTAAACAGGTGGTTCGAGGGTGGCAAAGTGTCACCTCCCTCT
	ATTCAGTTCGAAAGAGCTTACTACAAAAAAGACGATCAGATTCACGAGGACGCAGCCAAG
	AGAAAGATACGCTTCCAGATGCCAGCAACGGAATTAGTGCACGCCAGCGATGACGCTGGT
	TGGACCCCCAGCTACCTGCTGGGCATCGACCCCGGTGAGTACGGAATGGGTCTCAGTTTG
	GTGTCCATCAACAATGGAGAGGTCCTGGATTCTGGATTCATCCACATTAATTCCCTGATC
	AATTTCGCGTCCAAAAAAAGCAATCACCAGACCAAAGTAGTCCCCCGCCAGCAGTACAAG
	TCCCCCTACGCGAATTATCTCGAGCAGTCAAAGGATTCAGCAGCAGGGGATATAGCTCAC
	ATTCTGGATCGGCTAATCTACAAATTGAACGCCTTGCCTGTGTTCGAGGCGCTGTCTGGC
	AACAGTCAGAGTGCTGCTGATCAGGTATGGACCAAAGTTCTATCCTTCTATACATGGGGA
	GACAACGACGCACAGAACAGTATACGGAAGCAGCACTGGTTCGGTGCCTCACACTGGGAT
	ATTAAGGGGATGCTGCGCCAACCCCCAACCGAAAAAAAACCCAAACCATATATAGCCTTT
	CCCGGGAGTCAAGTGTCATCCTATGGAAATAGTCAAAGGTGTAGTTGTTGCGGCCGCAAT
	CCCATTGAGCAGTTGCGTGAGATGGCAAAGGACACGAGTATCAAGGAGCTGAAAATCCGA
	AATAGTGAGATCCAACTATTCGATGGTACAATCAAGCTGTTTAACCCCGACCCTTCCACC
	GTCATCGAGAGGCGGCGGCATAACCTAGGACCCTCACGCATTCCTGTGGCAGACCGAACT
	TTCAAGAATATTAGCCCTTCTTCGTTAGAGTTCAAGGAGCTCATTACTATCGTTTCTCGA
	AGCATCCGCCATAGCCCCGAATTTATTGCTAAGAAACGGGGTATCGGGTCTGAGTACTTT
	TGTGCTTATTCTGACTGCAACTCCTCACTGAACTCAGAGGCCAATGCCGCGGCCAATGTG
	GCACAGAAGTTTCAGAAGCAACTCTTTTTCGAACTCTGA

SEQ	ATGAAACGTATTCTGAACTCTCTGAAAGTCGCCGCACTGAGGCTGCTGTTTCGAGGAAAG
ID	GGCTCAGAGCTGGTGAAGACCGTCAAGTACCCTCTGGTTTCGCCCGTCCAGGGTGCTGTG
NO:	GAAGAACTCGCCGAAGCAATACGCCACGACAACCTACATTTATTTGGGCAGAAGGAAATC
163	GTAGATCTGATGGAGAAGGACGAGGGCACCCAGGTCTACTCGGTGGTGGACTTTTGGCTC
	GACACACTCCGTCTAGGGATGTTCTTCAGTCCAAGTGCTAATGCCCTTAAGATCACTCTG
	GGGAAGTTTAACAGCGACCAAGTTTCCCCTTTCAGGAAGGTTCTGGAGCAGTCCCCTTTC
	TTTCTCGCGGGTAGACTCAAAGTGGAGCCCGCTGAACGTATCCTCAGCGTGGAGATCCGC
	AAGATCGGTAAGAGGGAGAATAGAGTGGAGAACTACGCCGCAGATGTAGAGACTTGTTTT
	ATCGGTCAGCTGTCTAGTGATGAAAAGCAGTCTATCCAGAAGCTCGCTAACGATATCTGG
	GACTCTAAGGATCACGAAGAGCAAAGGATGCTTAAGGCGGATTTCTTTGCCATTCCCCTC
	ATCAAAGACCCAAAGGCAGTGACCGAGGAAGATCCCGAGAATGAAACCGCAGGCAAACAG
	AAGCCTCTCGAATTATGTGTGTGCTTAGTGCCCGAGTTGTACACCCGCGGGTTCGGTTCA
	ATAGCGGACTTCCTGGTCCAGCGTCTGACACTATTAAGAGACAAAATGAGCACAGACACA
	GCAGAAGACTGCCTTGAGTATGTCGGCATAGAGGAGGAGAAGGGTAATGGGATGAACTCG
	CTGCTGGGGACGTTCCTCAAGAACCTGCAGGGAGACGGGTTCGAACAGATCTTCCAATTT
	ATGCTCGGCAGTTACGTGGGATGGCAAGGTAAGGAAGACGTCCTACGCGAACGGCTTGAT
	TTGCTAGCGGAGAAGGTTAAAAGACTGCCGAAACCTAAGTTTGCCGGCGAGTGGTCCGGC
	CATCGGATGTTCCTGCATGGTCAATTGAAGAGCTGGTCCTCTAACTTTTTCCGCCTGTTT
	AACGAGACTAGGGAGCTCCTCGAAAGCATAAAATCCGACATCCAACACGCGACCATGTTA
	ATCAGCTACGTCGAAGAGAAAGGGGGATACCACCCACAACTCTTGTCACAGTACAGGAAA
	CTAATGGAGCAGCTGCCAGCTCTCAGAACAAAGGTGTTAGATCCAGAGATAGAAATGACT
	CACATGAGCGAGGCGGTAAGGTCGTACATTATGATCCACAAGTCGGTAGCAGGATTTCTG
	CCTGACTTACTCGAGTCCCTCGATAGGGACAAGGACAGGGAATTCCTGCTGAGTATATTT
	CCAAGGATCCCCAAAATTGACAAAAAAACTAAGGAAATCGTGGCCTGGGAGCTCCCAGGC
	GAGCCCGAAGAAGGATACCTGTTCACTGCCAATAATCTTTTTCGCAACTTTCTGGAGAAT
	CCTAAACATGTTCCACGTTTCATGGCAGAAAGGATCCCGGAAGATTGGACGCGCCTGCGG
	TCCGCTCCCGTATGGTTTGACGGCATGGTGAAACAATGGCAGAAAGTGGTAAACCAGCTG
	GTGGAGTCACCTGGAGCATTGTATCAGTTCAATGAAAGCTTTCTCCGACAACGTTTACAG
	GCAATGCTGACAGTGTATAAGAGAGACCTGCAGACAGAGAAATTCCTTAAGTTGTTGGCT
	GATGTCTGCAGGCCTCTGGTGGACTTCTTTGGGCTGGGGGGAAACGATATCATCTTCAAA
	AGCTGCCAGGACCCGAGGAAACAATGGCAAACTGTCATTCCCTTGAGTGTCCCCGCTGAT
	GTGTACACCGCGTGTGAGGGGCTGGCAATCCGGCTTCGTGAGACATTGGGATTTGAGTGG
	AAGAACCTTAAGGGCCATGAAAGGGAGGACTTTCTAAGACTGCACCAGCTTTTAGGGAAT
	CTGCTTTTCTGGATTCGAGATGCCAAACTGGTGGTGAAATTGGAAGATTGGATGAATAAT
	CCCTGTGTTCAGGAGTACGTTGAGGCTCGTAAGGCCATTGATCTCCCACTGGAGATCTTC
	GGCTTTGAGGTCCCCATCTTCCTGAACGGATATCTGTTTAGTGAACTGAGGCAGTTAGAA
	CTGCTGCTCCGCCGTAAGTCGGTTATGACCAGCTATTCGGTTAAGACAACTGGCAGTCCA
	AACAGGCTTTTCCAGTTAGTCTACCTGCCATTAAATCCTTCCGACCCTGAGAAAAAAAAT
	TCTAATAACTTTCAGGAACGCCTGGACACCCCCACTGGCTTATCACGTCGCTTCCTGGAC
	CTTACTCTGGACGCCTTCGCCGGCAAGTTGCTGACAGACCCCGTGACTCAAGAGCTTAAA
	ACTATGGCTGGGTTCTACGATCACCTGTTTGGTTTCAAGCTCCCATGTAAGCTGGCAGCC
	ATGTCTAACCACCCTGGCTCTAGCAGCAAGATGGTCGTGTTGGCCAAACCTAAAAAAGGG
	GTTGCATCTAATATAGGATTCGAACCAATCCCTGATCCCGCGCACCCCGTATTCCGGGTG
	AGATCATCATGGCCAGAGCTGAAGTATCTGGAGGGGTTACTGTATCTTCCAGAAGACACT
	CCACTGACAATAGAGCTCGCAGAGACAAGTGTTAGTTGTCAGAGCGTCAGTAGCGTGGCA
	TTCGATCTGAAAAATCTGACTACTATCCTTGGACGCGTGGGTGAGTTCCGTGTGACCGCA
	GACCAGCCTTTTAAGTTGACCCCCATCATCCCTGAGAAGGAGGAGTCCTTCATAGGAAAA
	ACATATCTAGGCCTTGATGCCGGGGAACGCTCAGGCGTAGGGTTCGCTATCGTCACAGTC
	GACGGGGATGGGTACGAGGTACAGCGCCTGGGGGTGCATGAAGATACACAGCTGATGGCC
	CTACAGCAGGTGGCCTCTAAAAGCTTGAAGGAGCCGGTGTTCCAGCCGCTCAGAAAGGGT
	ACTTTTCGGCAGCAGGAACGTATTAGAAAATCTCTCAGAGGATGTTATTGGAACTTCTAT
	CACGCTCTGATGATTAAGTACCGCGCCAAGGTAGTGCACGAAGAGAGCGTGGGCAGTTCC
	GGCCTGGTTGGGCAGTGGTTACGAGCATTCCAGAAGGACCTCAAGAAAGCCGATGTGTTG
	CCAAAAAAGGGAGGCAAAAACGGAGTCGATAAGAAAAAGAGAGAGTCTTCTGCACAAGAC
	ACATTGTGGGGAGGGGCTTTTAGCAAGAAGGAAGAACAGCAGATAGCTTTCGAAGTCCAA
	GCTGCTGGTTCTAGCCAGTTCTGCCTGAAGTGCGGATGGTGGTTCCAACTCGGAATGCGT
	GAGGTTAATCGCGTGCAGGAATCCGGCGTCGTGCTGGATTGGAATCGGAGTATTGTCACA
	TTCCTGATTGAGAGCTCTGGCGAGAAAGTGTATGGGTTCTCCCCTCAGCAACTCGAAAAG
	GGGTTCAGACCAGACATTGAAACCTTCAAGAAGATGGTTCGGGATTTCATGCGCCCGCCT
	ATGTTTGACCGGAAGGGTCGCCCAGCAGCTGCCTACGAAAGGTTTGTCTTGGGACGCCGG
	CATCGGCGGTATAGATTCGACAAGGTTTTTGAAGAACGATTCGGACGATCCGCGCTATTC
	ATTTGCCCGAGGGTTGGCTGTGGCAACTTTGACCACAGCAGCGAGCAGTCAGCCGTAGTG
	CTGGCTCTAATCGGATATATTGCCGACAAAGAGGGGATGAGCGGAAAAAAGCTAGTCTAC
	GTGCGTCTGGCAGAACTAATGGCGGAATGGAAATTGAAGAAACTGGAGAGGAGTAGAGTT
	GAGGAGCAAAGCTCCGCTCAGTGA

SEQ	ATGGCGGAGTCGAAGCAAATGCAGTGCAGGAAGTGTGGAGCCTCTATGAAGTACGAAGTG
ID	ATCGGCCTCGGGAAGAAAAGCTGCAGATATATGTGTCCCGACTGCGGGAATCACACATCT
NO:	GCAAGAAAGATTCAGAATAAGAAGAAAAGGGACAAGAAGTATGGATCTGCCAGTAAAGCA
164	CAAAGCCAACGAATCGCAGTTGCAGGGGCCTTATACCCGGATAAAAAGGTTCAGACCATC
	AAGACTTATAAGTATCCAGCCGACCTGAATGGTGAGGTCCATGACTCAGGGGTGGCCGAA
	AAAATAGCCCAAGCAATCCAGGAGGATGAAATAGGGCTCCTCGGCCCCTCTTCCGAGTAC
	GCCTGTTGGATCGCTAGCCAGAAACAGAGCGAGCCCTACAGTGTTGTAGACTTTTGGTTT
	GACGCTGTGTGCGCCGGAGGCGTGTTCGCCTATTCTGGGGCTAGATTGCTGTCTACCGTC
	CTGCAGCTATCTGGGGAGGAGAGCGTCCTACGCGCAGCCCTGGCATCCTCCCCTTTTGTC
	GACGATATCAATCTGGCACAGGCCGAAAAATTTCTGGCGGTGTCCAGGCGAACCGGCCAA
	GATAAGCTGGGGAAGCGCATTGGAGAGTGCTTCGCAGAGGGCCGACTTGAGGCCCTAGGC
	ATCAAGGACCGGATGCGTGAATTTGTCCAGGCTATCGATGTCGCTCAGACCGCTGGGCAG
	CGTTTTGCCGCGAAACTGAAAATCTTTGGGATTTCTCAGATGCCCGAGGCAAAGCAGTGG
	AACAATGACAGCGGACTCACCGTGTGCATCCTGCCCGACTATTACGTCCCAGAAGAAAAT
	CGCGCAGATCAGTTGGTCGTCCTGCTAAGACGACTGAGAGAGATAGCATACTGTATGGGG
	ATCGAAGATGAGGCCGGTTTTGAACATCTTGGAATTGATCCTGGCGCACTATCAAATTTT
	TCCAATGGCAATCCTAAACGCGGATTTTTGGGCCGCCTGCTGAACAATGATATTATTGCC
	TTAGCGAACAACATGTCCGCCATGACGCCTTACTGGGAGGGCAGGAAGGGAGAACTGATT
	GAAAGATTGGCTTGGCTGAAGCACCGTGCAGAGGGGCTTTATCTGAAGGAACCGCATTTT
	GGAAATAGTTGGGCCGACCATAGGTCTAGAATTTTTTCCAGAATAGCCGGGTGGCTTTCT
	GGGTGCGCTGGGAAGCTAAAGATCGCCAAAGACCAGATCAGCGGAGTGCGTACTGATCTG
	TTCCTTCTGAAGAGACTGCTGGATGCGGTCCCGCAGTCCGCCCCTTCTCCCGACTTCATA
	GCCTCTATCTCTGCCTTGGATCGCTTCCTGGAGGCCGCAGAATCTAGTCAGGATCCTGCC
	GAACAGGTGAGGGCCCTATACGCCTTTCATCTGAACGCACCCGCGGTGCGAAGCATCGCC
	AACAAGGCAGTCCAGCGATCCGACAGCCAAGAATGGCTTATAAAGGAACTGGACGCTGTG
	GACCACCTGGAGTTTAACAAGGCCTTTCCCTTCTTCTCTGATACGGGAAAGAAGAAAAAG
	AAAGGGGCTAACTCGAATGGCGCTCCGTCCGAGGAGGAGTACACCGAGACTGAGAGCATC
	CAGCAGCCCGAGGACGCTGAGCAAGAGGTTAATGGTCAGGAAGGCAACGGGGCCTCGAAG
	AACCAGAAGAAGTTTCAGAGAATCCCCCGATTCTTCGGCGAGGGGAGTCGCAGCGAGTAT
	CGCATCCTCACTGAAGCCCCGCAGTACTTCGACATGTTCTGTAACAACATGCGGGCCATC
	TTTATGCAATTAGAATCCCAACCGCGTAAAGCTCCCAGGGATTTTAAGTGTTTCCTGCAG
	AATCGGCTGCAGAAATTGTATAAGCAGACATTCCTGAACGCTCGATCCAACAAGTGCCGG
	GCATTACTAGAGTCCGTATTGATTAGTTGGGGAGAGTTTTACACCTACGGGGCTAACGAG
	AAAAAATTTCGACTGCGTCATGAAGCTTCTGAGCGCTCCTCGGACCCAGATTACGTGGTG
	CAACAGGCGCTGGAGATCGCTCGGAGGCTGTTTCTCTTCGGCTTTGAGTGGAGGGACTGT
	AGCGCAGGTGAAAGAGTGGATCTGGTCGAAATACATAAGAAAGCCATATCTTTCCTGTTG
	GCCATCACTCAGGCTGAGGTGTCTGTGGGCAGCTATAACTGGCTGGGCAATTCTACCGTG
	AGTCGGTACCTGTCCGTGGCAGGGACTGATACCCTTTACGGCACCCAGCTGGAAGAATTC
	TTAAATGCAACCGTGTTATCTCAGATGCGGGGGCTGGCTATCAGGTTATCATCTCAGGAA
	CTGAAGGATGGATTTGACGTACAGCTGGAGTCTAGTTGCCAGGATAATCTGCAACACTTG
	CTCGTGTACAGGGCTTCACGAGACCTTGCCGCCTGCAAGCGCGCTACTTGTCCAGCTGAG
	TTGGATCCTAAGATTCTGGTACTGCCCGTGGGGGCCTTTATCGCTAGCGTGATGAAAATG
	ATTGAAAGAGGGGATGAGCCTTTAGCTGGAGCTTATCTGAGACACAGACCCCATAGTTTC
	GGGTGGCAGATCCGCGTTCGAGGTGTGGCAGAGGTGGGAATGGACCAAGGGACCGCCCTG
	GCGTTCCAGAAACCGACCGAGAGCGAACCCTTCAAGATAAAGCCGTTTTCCGCTCAATAC
	GGCCCCGTTCTATGGCTGAACAGCTCCAGTTATAGCCAGAGCCAGTACCTGGACGGGTTC
	CTATCACAGCCCAAGAACTGGAGTATGCGGGTGCTGCCACAGGCCGGCTCAGTGCGGGTA
	GAACAGCGCGTCGCCTTGATTTGGAATCTCCAGGCCGGAAAGATGAGGCTGGAACGGAGC
	GGAGCGCGGGCTTTCTTCATGCCCGTCCCATTCAGTTTCCGCCCCAGTGGCAGCGGCGAC
	GAGGCAGTCCTGGCTCCAAATAGGTACCTGGGACTCTTTCCACACAGCGGCGGCATAGAG
	TACGCTGTGGTCGATGTTCTTGACTCTGCCGGCTTCAAAATACTCGAGAGAGGAACAATA
	GCCGTCAATGGCTTCTCCCAGAAACGAGGAGAAAGACAAGAGGAAGCCCATCGCGAAAAA
	CAAAGACGCGGTATCTCCGATATTGGGCGCAAGAAGCCAGTCCAGGCCGAAGTCGATGCG
	GCCAACGAGCTCCATCGAAAATACACCGATGTTGCTACTCGGCTGGGGTGTCGAATTGTC
	GTTCAATGGGCACCCCAACCCAAACCAGGCACTGCGCCGACCGCTCAGACTGTGTACGCT
	AGGGCCGTGAGGACTGAAGCACCAAGATCCGGCAATCAGGAAGATCACGCCAGGATGAAA
	TCTTCCTGGGGATACACATGGGGTACGTATTGGGAAAAAAGGAAGCCCGAGGACATCCTC
	GGCATTAGTACCCAGGTGTATTGGACAGGCGGGATCGGCGAGTCCTGCCCGGCTGTCGCC
	GTCGCGCTATTGGGACACATCAGGGCCACCTCAACCCAGACTGAATGGGAGAAAGAGGAA
	GTCGTGTTTGGGCGATTGAAAAAGTTCTTCCCATCCTGA

SEQ	ATGGAGAAGCGCATCAATAAAATTCGCAAGAAGCTGTCTGCCGATAACGCCACAAAACCA
ID	GTTAGTCGAAGCGGCCCAATGAAGACCCTGCTAGTTCGAGTGATGACTGATGATCTGAAG
NO:	AAAAGGCTCGAAAAGCGACGCAAGAAGCCTGAGGTAATGCCTCAGGTTATAAGTAACAAT
165	GCAGCAAACAATCTGCGGATGCTGCTTGACGATTACACAAAGATGAAGGAAGCCATTCTC
	CAGGTGTATTGGCAGGAGTTCAAGGATGATCACGTAGGCCTGATGTGTAAATTCGCGCAA
	CCTGCAAGCAAGAAGATCGACCAAAACAAGCTGAAACCCGAGATGGATGAAAAAGGCAAT
	TTAACAACCGCCGGATTCGCTTGTTCCCAGTGTGGGCAGCCACTGTTCGTGTACAAGTTA
	GAACAGGTGTCGGAAAAAGGAAAGGCATACACTAACTACTTTGGACGGTGCAATGTTGCA
	GAACACGAAAAGCTGATACTGCTTGCCCAGCTTAAGCCCGAAAAAGACAGCGACGAAGCG
	GTGACCTACAGCCTGGGAAAATTCGGGCAGCGGGCACTGGACTTCTATTCTATCCACGTT
	ACCAAGGAGAGCACCCACCCAGTGAAGCCGTTGGCCCAAATCGCTGGAAACCGGTACGCC
	AGCGGACCAGTCGGCAAGGCCCTGTCCGATGCCTGTATGGGCACAATTGCTTCTTTCCTG
	TCCAAGTACCAGGACATCATAATCGAGCACCAAAAAGTTGTGAAAGGGAATCAGAAACGC
	CTGGAATCCCTTCGAGAACTGGCCGGCAAGGAGAACCTTGAGTACCCGTCCGTGACCCTG
	CCTCCACAGCCACATACCAAAGAGGGCGTAGACGCGTATAATGAGGTCATTGCCCGCGTT
	CGCATGTGGGTTAATTTAAACCTGTGGCAGAAATTAAAACTAAGCCGAGATGATGCTAAA
	CCGTTACTGAGATTGAAGGGATTCCCTAGCTTTCCTGTGGTGGAGAGAAGGGAAAACGAG
	GTTGATTGGTGGAATACTATTAATGAGGTGAAAAAGCTTATTGACGCCAAGAGGGATATG
	GGCAGGGTGTTCTGGAGCGGGGTGACTGCCGAAAAGAGAAATACCATCCTCGAGGGATAC
	AATTACCTCCCCAACGAGAATGATCATAAGAAAAGAGAGGGGAGCTTAGAGAATCCAAAG
	AAACCTGCAAAGAGGCAATTCGGTGATCTCCTGCTCTACCTCGAGAAGAAATACGCGGGG
	GACTGGGGAAAAGTTTTTGACGAAGCCTGGGAGCGCATTGACAAGAAGATCGCCGGGCTG
	ACGTCTCACATTGAACGGGAAGAGGCACGGAATGCAGAGGACGCCCAGTCTAAGGCCGTG
	CTGACTGACTGGCTGCGCGCAAAGGCCTCCTTCGTGCTCGAACGTCTGAAGGAAATGGAT
	GAGAAAGAGTTTTACGCGTGTGAAATACAGCTGCAGAAGTGGTACGGCGATCTAAGGGGA
	AATCCCTTCGCAGTGGAAGCCGAGAATAGGGTAGTTGACATCAGTGGGTTCTCCATCGGC
	AGTGATGGACATTCTATCCAGTATAGAAACCTGCTCGCCTGGAAGTACTTAGAGAACGGC
	AAGAGAGAGTTCTATCTGCTGATGAACTACGGGAAAAAAGGTAGAATTCGCTTTACAGAT
	GGCACCGACATAAAGAAGTCCGGAAAGTGGCAAGGCCTCTTATACGGAGGCGGCAAAGCA
	AAGGTGATAGACTTGACTTTTGACCCTGACGACGAACAGCTGATAATCTTGCCGCTGGCC
	TTTGGCACAAGACAAGGTAGGGAATTTATCTGGAATGATCTTCTTTCTCTCGAGACCGGA
	CTCATCAAGCTCGCAAACGGAAGGGTCATCGAGAAGACAATCTACAATAAAAAGATAGGC
	CGAGACGAGCCAGCCCTGTTTGTGGCTTTGACATTTGAGCGGAGAGAGGTCGTAGATCCC
	AGCAACATCAAACCCGTGAACCTGATCGGTGTTGACAGGGGCGAGAACATCCCGGCGGTT
	ATCGCACTGACGGATCCAGAAGGATGTCCTCTGCCCGAGTTCAAAGATTCATCGGGAGGG
	CCAACCGACATTTTGAGGATAGGGGAGGGGTACAAGGAGAAGCAGCGAGCTATCCAGGCG
	GCCAAAGAAGTGGAGCAACGAAGAGCTGGTGGTTATTCTCGCAAGTTCGCTTCCAAAAGT
	CGTAACCTGGCTGACGATATGGTGCGCAATTCTGCCCGTGACCTTTTCTACCACGCCGTT
	ACACACGACGCCGTGTTAGTGTTTGAAAATCTTAGTCGAGGCTTCGGGCGACAGGGGAAG
	CGGACCTTTATGACCGAGAGACAGTATACAAAAATGGAGGATTGGCTGACCGCCAAACTG
	GCGTATGAAGGACTCACATCCAAGACCTATCTCTCAAAAACTTTGGCCCAGTATACATCT
	AAGACGTGCAGTAACTGTGGCTTCACCATTACCACAGCTGACTACGATGGCATGCTGGTC
	CGCTTAAAAAAGACATCTGACGGCTGGGCTACTACCCTCAACAATAAAGAGCTCAAAGCC
	GAAGGACAAATTACCTATTATAACAGGTATAAAAGACAGACTGTCGAGAAGGAGTTGAGC
	GCGGAGCTGGACCGCCTATCAGAGGAGTCAGGGAACAACGATATCTCTAAGTGGACTAAG
	GGACGCCGAGACGAGGCGTTGTTCTTGCTGAAAAAGCGGTTCTCTCATCGACCCGTGCAG
	GAGCAGTTCGTGTGTCTGGACTGCGGCCACGAGGTTCATGCTGATGAGCAAGCTGCTCTA
	AATATTGCCCGTAGTTGGTTGTTCCTGAACAGCAATTCAACAGAGTTCAAGTCATACAAG
	AGCGGAAAGCAGCCGTTTGTGGGCGCATGGCAGGCATTTTACAAAAGACGCCTGAAGGAA
	GTGTGGAAGCCAAACGCC

SEQ	ATGAAAAGGATTAACAAAATCCGAAGGCGGCTTGTAAAGGATTCTAACACCAAAAAGGCT
ID	GGCAAGACGGGGCCCATGAAAACATTACTCGTTAGAGTTATGACCCCCGACCTCAGAGAG
NO:	CGACTGGAAAATTTACGCAAGAAGCCAGAGAACATACCTCAGCCAATTAGTAATACCTCT
166	CGGGCAAACCTAAACAAGTTGCTTACTGATTACACGGAGATGAAAAAGGCCATACTGCAT
	GTGTACTGGGAGGAGTTTCAAAAGGACCCTGTCGGGCTAATGAGCAGGGTGGCTCAGCCT
	GCACCTAAAAACATCGACCAGCGGAAACTCATCCCAGTTAAGGACGGAAATGAGAGATTG
	ACAAGTTCAGGTTTCGCCTGCTCACAGTGCTGTCAACCGCTGTACGTTTATAAGTTAGAA
	CAAGTGAATGACAAAGGAAAGCCTCACACAAATTATTTTGGCCGGTGTAATGTCTCTGAG
	CATGAGCGTCTGATTCTGTTGTCCCCGCATAAACCGGAAGCTAATGACGAGCTCGTAACC
	TACAGCTTGGGGAAGTTTGGCCAAAGAGCATTGGACTTCTATTCAATCCATGTGACCCGC
	GAATCCAATCATCCCGTCAAGCCCTTGGAGCAGATAGGGGGCAATAGTTGCGCTTCTGGC
	CCTGTGGGCAAAGCCCTGTCCGACGCCTGTATGGGAGCCGTGGCTTCATTCCTGACCAAA
	TATCAGGATATCATCTTGGAGCACCAGAAAGTGATCAAGAAAAATGAAAAAAGGTTAGCA
	AACCTCAAGGATATTGCAAGCGCTAACGGCTTGGCTTTTCCTAAAATCACACTTCCACCT
	CAGCCTCACACAAAGGAAGGCATCGAGGCATACAACAATGTGGTGGCCCAGATCGTCATC
	TGGGTTAACTTAAACCTGTGGCAGAAACTTAAAATTGGCAGGGATGAGGCAAAACCCTTA
	CAGCGCCTGAAAGGATTCCCCAGCTTTCCACTGGTGGAGCGCCAGGCTAACGAAGTGGAC
	TGGTGGGATATGGTGTGTAACGTCAAGAAGCTCATCAATGAAAAGAAAGAGGACGGTAAA
	GTCTTCTGGCAGAACCTCGCCGGTTACAAACGGCAGGAGGCGCTGTTACCTTATCTGTCG
	AGTGAAGAGGACCGGAAAAAAGGCAAGAAATTTGCTCGTTATCAGTTTGGTGATTTGCTC
	CTACATTTGGAGAAGAAGCACGGCGAGGACTGGGGAAAAGTATACGATGAGGCCTGGGAG
	AGGATTGACAAAAAGGTGGAGGGACTGTCAAAGCACATCAAGCTCGAAGAAGAGCGCAGA
	AGCGAGGACGCCCAATCCAAAGCAGCGCTGACTGACTGGCTGCGGGCGAAGGCCAGTTTT
	GTAATCGAAGGCCTTAAAGAAGCCGACAAGGATGAATTCTGCAGATGCGAATTAAAACTC
	CAGAAGTGGTACGGCGATCTCCGAGGTAAGCCTTTCGCAATCGAGGCCGAGAATTCCATA
	CTGGACATTAGTGGATTCAGTAAACAGTATAATTGTGCCTTTATATGGCAGAAGGATGGT
	GTCAAGAAACTCAACCTGTACCTTATTATTAATTATTTCAAAGGCGGGAAACTGAGATTT
	AAGAAGATAAAGCCTGAAGCCTTTGAGGCGAACCGATTCTACACAGTTATTAACAAGAAA
	TCTGGTGAAATTGTACCCATGGAGGTAAACTTCAACTTCGATGATCCCAATCTGATTATA
	TTGCCACTAGCTTTTGGCAAGCGGCAGGGTAGGGAATTCATTTGGAACGATTTGCTTTCA
	CTGGAAACAGGGTCCCTTAAGCTGGCAAACGGGAGAGTGATTGAAAAGACATTGTACAAT
	CGGAGGACACGTCAGGATGAACCTGCCCTTTTCGTGGCTCTGACATTCGAGCGCAGGGAG
	GTTCTGGACTCTAGCAATATCAAGCCAATGAACCTGATCGGCATAGACCGAGGAGAGAAT
	ATTCCGGCTGTGATCGCACTCACCGATCCCGAAGGATGTCCCCTTTCTCGGTTCAAGGAC
	TCCTTAGGCAATCCAACTCATATCCTGAGAATCGGCGAGTCATACAAGGAGAAGCAGCGA
	ACAATTCAGGCCGCCAAGGAAGTCGAGCAGAGGCGAGCTGGCGGCTACAGCCGTAAATAC
	GCTAGTAAAGCTAAGAACCTGGCCGACGATATGGTGCGCAATACTGCTAGAGACCTGCTG
	TACTATGCAGTGACGCAGGACGCAATGCTGATATTCGAGAATCTGTCCAGAGGATTCGGA
	AGGCAGGGCAAGCGGACGTTCATGGCCGAGCGCCAGTATACAAGGATGGAGGATTGGTTA
	ACGGCCAAGCTTGCCTATGAGGGGCTACCTAGTAAGACCTATCTGTCTAAGACGCTGGCT
	CAATACACCAGTAAGACCTGCTCAAACTGTGGCTTTACAATCACTTCTGCTGATTATGAT
	AGAGTGCTCGAGAAGCTAAAAAAAACTGCCACCGGCTGGATGACTACTATTAATGGGAAG
	GAACTGAAAGTGGAAGGACAGATTACCTATTATAATCGCTACAAGCGTCAAAACGTCGTC
	AAGGACCTGTCGGTGGAATTGGACAGACTCAGTGAAGAGTCCGTGAACAATGATATCAGC
	TCCTGGACAAAAGGGCGCAGTGGGGAGGCACTCAGCTTGCTTAAAAAGAGGTTTTCACAT
	CGGCCGGTCCAGGAGAAATTTGTCTGCCTGAACTGCGGATTCGAGACACACGCCGACGAG
	CAGGCAGCACTGAACATTGCCAGATCCTGGCTGTTCCTTAGGTCCCAGGAATATAAGAAG
	TACCAGACTAACAAAACCACGGGAAACACAGATAAAAGGGCCTTTGTCGAAACTTGGCAA
	TCCTTTTACCGGAAGAAGTTAAAGGAAGTGTGGAAGCCC

SEQ	ATGGATAAGAAATACTCAATAGGCTTAGCAATCGGCACAAATAGCGTCGGATGGGCGGTG
ID	ATCACTGATGAATATAAGGTTCCGTCTAAAAAGTTCAAGGTTCTGGGAAATACAGACCGC
NO:	CACAGTATCAAAAAAAATCTTATAGGGGCTCTTTTATTTGACAGTGGAGAGACAGCGGAA
167	GCGACTCGTCTCAAACGGACAGCTCGTAGAAGGTATACACGTCGGAAGAATCGTATTTGT
	TATCTACAGGAGATTTTTTCAAATGAGATGGCGAAAGTAGATGATAGTTTCTTTCATCGA
	CTTGAAGAGTCTTTTTTGGTGGAAGAAGACAAGAAGCATGAACGTCATCCTATTTTTGGA
	AATATAGTAGATGAAGTTGCTTATCATGAGAAATATCCAACTATCTATCATCTGCGAAAA
	AAATTGGTAGATTCTACTGATAAAGCGGATTTGCGCTTAATCTATTTGGCCTTAGCGCAT
	ATGATTAAGTTTCGTGGTCATTTTTTGATTGAGGGAGATTTAAATCCTGATAATAGTGAT
	GTGGACAAACTATTTATCCAGTTGGTACAAACCTACAATCAATTATTTGAAGAAAACCCT
	ATTAACGCAAGTGGAGTAGATGCTAAAGCGATTCTTTCTGCACGATTGAGTAAATCAAGA
	CGATTAGAAAATCTCATTGCTCAGCTCCCCGGTGAGAAGAAAAATGGCTTATTTGGGAAT
	CTCATTGCTTTGTCATTGGGTTTGACCCCTAATTTTAAATCAAATTTTGATTTGGCAGAA
	GATGCTAAATTACAGCTTTCAAAAGATACTTACGATGATGATTTAGATAATTTATTGGCG
	CAAATTGGAGATCAATATGCTGATTTGTTTTTGGCAGCTAAGAATTTATCAGATGCTATT
	TTACTTTCAGATATCCTAAGAGTAAATACTGAAATAACTAAGGCTCCCCTATCAGCTTCA
	ATGATTAAACGCTACGATGAACATCATCAAGACTTGACTCTTTTAAAAGCTTTAGTTCGA
	CAACAACTTCCAGAAAAGTATAAAGAAATCTTTTTTGATCAATCAAAAAACGGATATGCA
	GGTTATATTGATGGGGGAGCTAGCCAAGAAGAATTTTATAAATTTATCAAACCAATTTTA
	GAAAAAATGGATGGTACTGAGGAATTATTGGTGAAACTAAATCGTGAAGATTTGCTGCGC
	AAGCAACGGACCTTTGACAACGGCTCTATTCCCCATCAAATTCACTTGGGTGAGCTGCAT
	GCTATTTTGAGAAGACAAGAAGACTTTTATCCATTTTTAAAAGACAATCGTGAGAAGATT
	GAAAAAATCTTGACTTTTCGAATTCCTTATTATGTTGGTCCATTGGCGCGTGGCAATAGT
	CGTTTTGCATGGATGACTCGGAAGTCTGAAGAAACAATTACCCCATGGAATTTTGAAGAA
	GTTGTCGATAAAGGTGCTTCAGCTCAATCATTTATTGAACGCATGACAAACTTTGATAAA
	AATCTTCCAAATGAAAAAGTACTACCAAAACATAGTTTGCTTTATGAGTATTTTACGGTT
	TATAACGAATTGACAAAGGTCAAATATGTTACTGAAGGAATGCGAAAACCAGCATTTCTT
	TCAGGTGAACAGAAGAAAGCCATTGTTGATTTACTCTTCAAAACAAATCGAAAAGTAACC
	GTTAAGCAATTAAAAGAAGATTATTTCAAAAAAATAGAATGTTTTGATAGTGTTGAAATT
	TCAGGAGTTGAAGATAGATTTAATGCTTCATTAGGTACCTACCATGATTTGCTAAAAATT
	ATTAAAGATAAAGATTTTTTGGATAATGAAGAAAATGAAGATATCTTAGAGGATATTGTT
	TTAACATTGACCTTATTTGAAGATAGGGAGATGATTGAGGAAAGACTTAAAACATATGCT
	CACCTCTTTGATGATAAGGTGATGAAACAGCTTAAACGTCGCCGTTATACTGGTTGGGGA
	CGTTTGTCTCGAAAATTGATTAATGGTATTAGGGATAAGCAATCTGGCAAAACAATATTA
	GATTTTTTGAAATCAGATGGTTTTGCCAATCGCAATTTTATGCAGCTGATCCATGATGAT
	AGTTTGACATTTAAAGAAGACATTCAAAAAGCACAAGTGTCTGGACAAGGCGATAGTTTA
	CATGAACATATTGCAAATTTAGCTGGTAGCCCTGCTATTAAAAAAGGTATTTTACAGACT
	GTAAAAGTTGTTGATGAATTGGTCAAAGTAATGGGGCGGCATAAGCCAGAAAATATCGTT
	ATTGAAATGGCACGTGAAAATCAGACAACTCAAAAGGGCCAGAAAAATTCGCGAGAGCGT
	ATGAAACGAATCGAAGAAGGTATCAAAGAATTAGGAAGTCAGATTCTTAAAGAGCATCCT
	GTTGAAAATACTCAATTGCAAAATGAAAAGCTCTATCTCTATTATCTCCAAAATGGAAGA
	GACATGTATGTGGACCAAGAATTAGATATTAATCGTTTAAGTGATTATGATGTCGATCAC
	ATTGTTCCACAAAGTTTCCTTAAAGACGATTCAATAGACAATAAGGTCTTAACGCGTTCT
	GATAAAAATCGTGGTAAATCGGATAACGTTCCAAGTGAAGAAGTAGTCAAAAAGATGAAA
	AACTATTGGAGACAACTTCTAAACGCCAAGTTAATCACTCAACGTAAGTTTGATAATTTA
	ACGAAAGCTGAACGTGGAGGTTTGAGTGAACTTGATAAAGCTGGTTTTATCAAACGCCAA
	TTGGTTGAAACTCGCCAAATCACTAAGCATGTGGCACAAATTTTGGATAGTCGCATGAAT
	ACTAAATACGATGAAAATGATAAACTTATTCGAGAGGTTAAAGTGATTACCTTAAAATCT
	AAATTAGTTTCTGACTTCCGAAAAGATTTCCAATTCTATAAAGTACGTGAGATTAACAAT
	TACCATCATGCCCATGATGCGTATCTAAATGCCGTCGTTGGAACTGCTTTGATTAAGAAA
	TATCCAAAACTTGAATCGGAGTTTGTCTATGGTGATTATAAAGTTTATGATGTTCGTAAA
	ATGATTGCTAAGTCTGAGCAAGAAATAGGCAAAGCAACCGCAAAATATTTCTTTTACTCT
	AATATCATGAACTTCTTCAAAACAGAAATTACACTTGCAAATGGAGAGATTCGCAAACGC
	CCTCTAATCGAAACTAATGGGGAAACTGGAGAAATTGTCTGGGATAAAGGGCGAGATTTT
	GCCACAGTGCGCAAAGTATTGTCCATGCCCCAAGTCAATATTGTCAAGAAAACAGAAGTA
	CAGACAGGCGGATTCTCCAAGGAGTCAATTTTACCAAAAAGAAATTCGGACAAGCTTATT
	GCTCGTAAAAAAGACTGGGATCCAAAAAAATATGGTGGTTTTGATAGTCCAACGGTAGCT
	TATTCAGTCCTAGTGGTTGCTAAGGTGGAAAAAGGGAAATCGAAGAAGTTAAAATCCGTT
	AAAGAGTTACTAGGGATCACAATTATGGAAAGAAGTTCCTTTGAAAAAAATCCGATTGAC
	TTTTTAGAAGCTAAAGGATATAAGGAAGTTAAAAAAGACTTAATCATTAAACTACCTAAA
	TATAGTCTTTTTGAGTTAGAAAACGGTCGTAAACGGATGCTGGCTAGTGCCGGAGAATTA
	CAAAAAGGAAATGAGCTGGCTCTGCCAAGCAAATATGTGAATTTTTTATATTTAGCTAGT
	CATTATGAAAAGTTGAAGGGTAGTCCAGAAGATAACGAACAAAAACAATTGTTTGTGGAG
	CAGCATAAGCATTATTTAGATGAGATTATTGAGCAAATCAGTGAATTTTCTAAGCGTGTT
	ATTTTAGCAGATGCCAATTTAGATAAAGTTCTTAGTGCATATAACAAACATAGAGACAAA
	CCAATACGTGAACAAGCAGAAAATATTATTCATTTATTTACGTTGACGAATCTTGGAGCT
	CCCGCTGCTTTTAAATATTTTGATACAACAATTGATCGTAAACGATATACGTCTACAAAA
	GAAGTTTTAGATGCCACTCTTATCCATCAATCCATCACTGGTCTTTATGAAACACGCATT
	GATTTGAGTCAGCTAGGAGGTGACTGA

SEQ	ATGGATAAGAAGTATTCAATTGGACTTGCGATTGGCACTAACAGTGTGGGCTGGGCGGTG
ID	ATTACAGACGAGTATAAGGTGCCGTCAAAAAAGTTTAAAGTTCTGGGCAACACTGATCGC
NO:	CATTCCATCAAGAAAAACCTAATCGGGGCCCTTCTTTTTGATAGTGGCGAAACGGCCGAG
168	GCGACGCGTCTAAAACGTACCGCGCGGCGTCGCTACACCCGACGAAAAAACCGTATTTGT
	TACCTTCAGGAGATCTTCAGTAACGAAATGGCTAAGGTGGACGATTCATTCTTCCACCGT
	CTGGAGGAGTCCTTTTTAGTTGAAGAAGACAAGAAGCATGAGCGACACCCAATTTTTGGT
	AACATTGTCGACGAAGTCGCCTATCACGAAAAATATCCGACCATTTATCACCTGCGCAAA
	AAACTGGTCGATAGCACGGATAAAGCGGATCTGCGGCTTATTTACCTGGCGCTTGCCCAC
	ATGATCAAGTTCCGCGGCCACTTCCTGATAGAAGGAGACCTGAACCCGGATAATAGCGAT
	GTAGACAAACTGTTTATTCAGCTGGTCCAGACCTACAACCAGCTGTTTGAAGAAAATCCG
	ATTAATGCGTCAGGCGTGGATGCGAAAGCGATACTGAGTGCCCGCCTGTCGAAATCTCGC
	CGTCTCGAAAATCTGATTGCACAGCTGCCCGGCGAAAAAAAAAACGGTCTTTTTGGCAAT
	CTGATCGCGCTGTCACTGGGCCTGACACCAAATTTTAAGAGCAACTTCGACCTGGCAGAG
	GATGCGAAGCTTCAACTGTCGAAGGACACCTATGACGATGATCTGGATAATCTTCTGGCA
	CAAATCGGTGATCAGTATGCGGATTTATTCCTTGCAGCGAAAAACCTATCTGACGCAATT
	CTGTTGAGCGATATCCTCCGCGTCAACACCGAAATCACTAAAGCCCCCCTGTCAGCGTCG
	ATGATTAAACGTTATGATGAGCACCATCAGGATCTGACCTTGCTAAAGGCGCTGGTGCGA
	CAGCAGCTTCCCGAAAAATATAAAGAGATCTTTTTTGATCAATCGAAGAATGGTTATGCC
	GGATACATTGATGGCGGAGCCAGTCAGGAAGAATTTTACAAATTCATCAAACCGATCCTG
	GAAAAAATGGATGGCACAGAAGAACTGCTTGTGAAATTGAACCGGGAAGATTTACTGCGC
	AAACAGCGTACGTTCGACAACGGCTCCATACCCCATCAGATTCACTTAGGTGAGCTGCAT
	GCAATACTCCGTCGCCAGGAAGATTTTTATCCATTTTTAAAAGACAACCGTGAGAAGATT
	GAAAAAATTTTAACTTTTCGTATTCCATATTACGTCGGGCCTTTGGCCCGAGGTAACTCT
	CGATTCGCCTGGATGACGAGAAAAAGCGAGGAGACCATCACTCCGTGGAATTTTGAAGAG
	GTTGTTGATAAAGGCGCGAGCGCCCAGTCGTTTATCGAACGTATGACCAACTTTGATAAA
	AATCTGCCGAATGAAAAAGTGCTTCCGAAGCATTCTCTGTTGTATGAATATTTCACTGTG
	TACAATGAGTTAACGAAAGTGAAATATGTGACCGAAGGCATGCGGAAACCTGCTTTTCTG
	TCCGGAGAACAGAAAAAAGCAATTGTGGACCTGCTGTTCAAAACGAACCGGAAAGTAACT
	GTGAAGCAGCTGAAAGAGGACTACTTCAAAAAAATCGAATGCTTCGACTCAGTAGAGATC
	TCTGGTGTTGAAGATCGCTTCAACGCGAGTCTGGGAACGTACCATGATTTGTTGAAAATC
	ATCAAAGATAAAGACTTTCTGGATAACGAAGAGAATGAGGACATTCTTGAAGATATTGTT
	TTGACACTGACTCTGTTTGAGGATCGCGAAATGATTGAAGAGCGCCTGAAAACGTATGCC
	CATTTATTCGATGACAAAGTCATGAAGCAGCTGAAACGTCGCCGCTATACTGGGTGGGGC
	AGACTTTCACGTAAATTGATCAATGGTATAAGAGACAAACAGAGCGGCAAAACTATCTTA
	GATTTCCTGAAGAGTGATGGATTTGCCAACCGGAATTTTATGCAGCTTATACATGATGAC
	TCGCTAACGTTTAAAGAAGACATTCAGAAGGCGCAGGTCAGCGGCCAGGGTGATTCGCTG
	CATGAACACATTGCAAATCTTGCCGGATCGCCAGCGATCAAAAAAGGCATCCTTCAGACA
	GTAAAAGTTGTGGATGAACTGGTGAAAGTAATGGGTCGTCACAAGCCAGAAAATATTGTG
	ATCGAAATGGCCCGGGAAAATCAGACTACTCAAAAAGGTCAGAAAAATTCTCGCGAGCGT
	ATGAAACGTATTGAAGAAGGCATCAAAGAGCTAGGCAGCCAGATATTAAAGGAACATCCG
	GTTGAGAACACTCAGCTGCAGAATGAAAAACTGTATCTGTATTATCTTCAGAACGGCCGT
	GACATGTATGTTGATCAAGAACTGGATATCAATCGCTTGTCCGATTATGACGTGGATCAT
	ATTGTTCCGCAAAGCTTTCTGAAAGACGATTCTATTGACAATAAAGTACTGACACGTTCG
	GACAAAAACCGTGGTAAAAGCGATAACGTACCGTCGGAAGAAGTTGTTAAGAAAATGAAA
	AATTATTGGCGCCAACTCCTGAATGCTAAATTGATTACCCAGCGGAAATTTGATAACTTA
	ACCAAAGCCGAGCGGGGTGGCTTAAGTGAACTGGATAAAGCGGGTTTTATTAAACGCCAA
	CTGGTAGAAACCCGCCAGATAACGAAACATGTAGCTCAAATCCTCGATAGTCGCATGAAT
	ACGAAATATGACGAAAATGATAAATTGATCCGTGAAGTAAAAGTGATTACTCTTAAAAGC
	AAATTGGTATCTGATTTTCGGAAAGATTTCCAATTCTATAAGGTGAGAGAAATTAACAAT
	TACCATCATGCACATGATGCGTATTTAAATGCAGTTGTTGGCACCGCCTTAATCAAAAAA
	TATCCGAAATTAGAATCTGAGTTCGTGTATGGTGATTATAAAGTTTATGATGTTCGAAAA
	ATGATTGCTAAGTCTGAACAGGAAATCGGCAAAGCGACCGCAAAGTATTTTTTTTATAGC
	AATATTATGAATTTTTTTAAAACTGAGATTACCCTGGCGAATGGCGAAATTCGCAAACGT
	CCTCTGATTGAAACCAATGGCGAAACCGGCGAGATAGTATGGGACAAGGGCCGTGATTTT
	GCGACCGTCCGGAAAGTCCTGTCAATGCCGCAGGTGAATATTGTCAAGAAAACAGAAGTT
	CAGACAGGCGGTTTTAGTAAAGAGTCTATTCTGCCCAAACGTAATTCGGATAAATTGATT
	GCCCGCAAGAAAGATTGGGATCCGAAGAAATATGGTGGATTCGATTCTCCGACGGTCGCC
	TATAGCGTTCTAGTCGTCGCCAAGGTCGAAAAAGGTAAATCCAAAAAACTGAAATCTGTG
	AAAGAACTGTTAGGCATTACAATCATGGAACGTAGTAGTTTTGAAAAGAACCCGATCGAC
	TTCCTCGAGGCGAAAGGCTACAAAGAAGTCAAGAAGGATTTGATTATTAAACTCCCAAAA
	TATTCATTATTTGAGTTAGAAAACGGTAGGAAGCGTATGCTGGCGAGTGCTGGGGAATTA
	CAGAAAGGGAATGAGTTAGCACTGCCGTCAAAATATGTGAACTTTCTGTATCTGGCCTCC
	CATTACGAGAAACTGAAAGGTAGCCCGGAAGATAATGAACAGAAACAACTATTTGTCGAG
	CAACACAAACATTATCTGGATGAAATTATTGAACAGATTAGTGAATTCTCTAAACGTGTT
	ATTTTAGCGGATGCCAACCTTGACAAGGTGCTGAGCGCATATAATAAACACCGTGATAAA
	CCCATTCGTGAACAGGCTGAAAATATCATACATCTGTTCACGTTAACCAACTTGGGAGCT
	CCTGCCGCTTTTAAATATTTCGATACCACAATTGACCGCAAACGTTATACGTCTACAAAA
	GAGGTGCTCGATGCGACCCTGATCCACCAGTCTATTACAGGCCTGTATGAAACTCGTATC
	GACCTGTCACAACTGGGCGGCGACTGA

SEQ	ATGGACAAGAAATATTCAATCGGTTTAGCAATAGGAACTAACTCAGTAGGTTGGGCTGTA
ID	ATTACAGACGAATACAAGGTACCGTCCAAAAAGTTTAAGGTGTTGGGGAACACAGATAGA
NO:	CACTCTATAAAAAAAAATTTAATAGGCGCTTTACTTTTCGATTCAGGCGAAACTGCAGAA
169	GCGACACGTCTGAAGAGAACCGCTAGACGTAGATACACGAGGAGAAAGAACAGAATATGT
	TACCTACAAGAAATTTTTTCTAATGAGATGGCTAAGGTGGATGATTCGTTTTTTCATAGA
	CTCGAAGAATCTTTCTTAGTTGAAGAAGATAAAAAACACGAAAGGCATCCTATCTTTGGA
	AACATAGTTGATGAGGTGGCTTACCATGAAAAATATCCCACTATATATCACCTTAGAAAA
	AAGTTGGTTGATTCAACCGACAAAGCGGATCTAAGGTTAATTTACCTCGCGTTGGCTCAC
	ATGATAAAATTTAGAGGACATTTCTTGATCGAAGGTGATTTAAATCCCGATAACTCTGAT
	GTAGATAAACTGTTCATCCAGTTGGTTCAAACATATAATCAGTTGTTCGAAGAGAACCCC
	ATTAACGCATCAGGTGTTGATGCTAAAGCAATCTTATCAGCAAGGTTGAGCAAGAGCAGA
	CGTCTGGAAAACTTGATTGCCCAATTGCCAGGTGAAAAGAAGAACGGTCTTTTTGGAAAT
	TTAATTGCACTTTCACTTGGGTTGACACCGAATTTTAAAAGCAATTTCGACCTCGCTGAG
	GATGCTAAACTCCAGTTATCTAAGGATACATATGACGATGATTTGGATAATCTATTGGCC
	CAGATAGGTGATCAGTATGCAGATTTGTTTTTGGCAGCTAAGAATTTATCAGATGCAATT
	CTACTGAGCGATATTTTAAGGGTGAATACAGAAATAACTAAAGCACCTTTGTCTGCATCT
	ATGATAAAAAGATACGATGAACACCATCAAGATCTCACACTATTAAAAGCTTTAGTTAGA
	CAACAATTACCAGAAAAATATAAAGAAATCTTTTTCGATCAGTCCAAGAACGGATACGCC
	GGCTATATAGATGGCGGTGCCTCCCAAGAAGAATTTTACAAATTTATCAAACCCATTTTG
	GAAAAGATGGATGGTACTGAAGAATTATTGGTCAAATTAAACAGGGAAGATTTATTAAGA
	AAACAAAGGACCTTTGATAATGGTTCTATTCCACACCAAATCCATCTAGGGGAATTACAT
	GCGATTCTTAGAAGACAAGAAGATTTTTATCCATTCTTGAAAGATAACAGGGAAAAGATA
	GAGAAAATCTTAACTTTTAGAATTCCCTACTACGTCGGGCCCTTAGCTAGGGGGAATTCT
	AGATTCGCCTGGATGACACGCAAATCAGAAGAAACAATTACGCCTTGGAATTTTGAAGAA
	GTTGTTGATAAAGGAGCCTCTGCTCAATCTTTTATTGAACGAATGACCAATTTTGATAAG
	AATTTACCCAATGAAAAGGTCTTACCCAAACATTCACTCCTATACGAGTACTTTACTGTT
	TACAATGAGTTGACAAAAGTGAAGTATGTTACCGAGGGTATGCGAAAACCTGCTTTCTTG
	AGTGGTGAACAAAAGAAGGCCATTGTTGACTTGTTATTCAAAACTAACAGAAAGGTCACT
	GTGAAGCAGCTTAAAGAAGATTATTTCAAAAAGATCGAATGTTTCGACTCGGTAGAAATT
	AGTGGTGTGGAAGATAGATTTAATGCTTCTCTTGGAACATATCATGATCTACTAAAGATC
	ATCAAAGATAAAGATTTCTTGGACAATGAAGAAAATGAAGATATTCTTGAAGACATCGTG
	TTGACACTTACATTGTTTGAGGACAGAGAAATGATTGAAGAAAGGCTGAAGACCTACGCC
	CATTTGTTTGATGATAAAGTCATGAAACAGTTAAAGAGGAGAAGGTATACCGGATGGGGT
	AGGCTGTCTCGCAAATTGATTAATGGTATTCGTGATAAACAATCGGGTAAAACAATCCTA
	GATTTCCTGAAGTCCGATGGTTTCGCCAACAGGAATTTTATGCAATTGATTCATGACGAT
	TCTTTGACTTTTAAAGAGGATATTCAGAAAGCACAGGTCTCAGGACAGGGCGATTCACTC
	CATGAACATATAGCTAACCTGGCTGGCTCCCCTGCTATTAAGAAAGGTATCTTGCAAACC
	GTCAAAGTAGTAGACGAACTTGTTAAAGTTATGGGAAGACACAAACCTGAAAATATCGTT
	ATTGAAATGGCTCGCGAAAACCAGACAACACAAAAGGGTCAAAAGAATTCGAGAGAGAGA
	ATGAAGCGTATCGAAGAAGGTATTAAAGAACTTGGGTCCCAAATACTTAAAGAACATCCA
	GTAGAAAACACTCAGCTTCAAAATGAAAAATTATACTTATATTATCTTCAGAATGGCCGC
	GATATGTATGTTGACCAAGAGTTAGATATAAATAGGTTGTCTGATTACGACGTGGATCAT
	ATTGTACCTCAATCTTTTCTAAAAGATGATTCAATTGATAATAAGGTATTAACGAGAAGT
	GATAAAAATAGAGGTAAATCTGACAACGTGCCAAGCGAAGAGGTGGTGAAGAAAATGAAA
	AATTATTGGCGTCAACTGTTGAACGCCAAGTTAATTACGCAGAGAAAGTTTGATAATCTA
	ACAAAAGCTGAAAGAGGAGGCCTATCTGAGTTAGATAAGGCCGGTTTTATCAAACGTCAG
	TTAGTTGAAACCAGGCAAATCACGAAGCACGTTGCCCAAATTCTAGATTCAAGGATGAAT
	ACCAAATACGATGAAAACGATAAACTGATTCGGGAAGTCAAGGTTATAACTCTAAAAAGC
	AAACTAGTTTCAGATTTTCGCAAAGATTTTCAATTTTACAAAGTTCGAGAAATCAATAAT
	TATCATCATGCTCACGACGCGTACTTGAACGCGGTCGTTGGTACAGCTTTAATAAAGAAA
	TATCCTAAACTGGAATCGGAATTTGTATATGGGGATTACAAAGTATACGACGTGAGAAAG
	ATGATCGCTAAATCTGAACAAGAAATTGGGAAAGCAACTGCCAAATATTTTTTTTACAGC
	AACATAATGAATTTTTTTAAAACGGAAATTACATTGGCAAATGGCGAAATTAGAAAGCGC
	CCATTGATAGAGACCAATGGAGAGACTGGGGAAATCGTGTGGGATAAAGGACGTGATTTT
	GCCACAGTGAGGAAAGTGTTAAGTATGCCACAAGTTAATATTGTAAAAAAGACCGAGGTC
	CAAACGGGTGGATTTAGCAAAGAATCAATTTTACCTAAGAGAAATTCAGATAAATTAATT
	GCCCGCAAAAAGGATTGGGATCCTAAAAAATATGGTGGTTTTGATTCCCCAACAGTTGCT
	TACTCCGTCCTAGTTGTTGCTAAGGTTGAAAAAGGAAAGTCTAAGAAACTTAAATCCGTA
	AAAGAGTTACTGGGAATTACAATAATGGAAAGATCCTCTTTCGAAAAGAACCCTATTGAC
	TTCTTGGAGGCGAAAGGTTATAAAGAAGTCAAAAAAGATTTGATCATAAAACTACCAAAG
	TATTCTCTATTTGAATTGGAAAACGGCAGAAAAAGGATGTTGGCAAGCGCTGGTGAACTA
	CAAAAGGGTAACGAATTGGCATTGCCGAGTAAATACGTGAATTTTCTATATTTGGCATCA
	CATTACGAAAAGTTAAAGGGATCACCCGAGGATAACGAGCAGAAACAACTGTTTGTTGAA
	CAACACAAACATTATCTTGATGAAATTATAGAACAAATTAGTGAGTTCAGTAAGAGAGTT
	ATTTTAGCCGATGCAAATTTAGACAAAGTTTTATCTGCTTATAACAAACATAGAGATAAG
	CCTATAAGGGAACAAGCCGAAAATATTATTCATTTGTTTACGTTAACAAATTTAGGGGCA
	CCAGCAGCATTCAAGTACTTCGATACGACTATCGATCGTAAGCGTTACACATCTACCAAA
	GAAGTTCTTGATGCAACTTTGATTCATCAATCTATAACAGGCTTATATGAAACTAGAATC
	GATCTGTCACAACTTGGTGGTGACTAA

SEQ	ATGGACAAGAAGTACTCAATTGGGCTTGCTATCGGCACTAACAGCGTTGGCTGGGCGGTC
ID	ATCACAGACGAATATAAGGTCCCATCAAAGAAATTCAAAGTCCTTGGCAATACGGACCGA
NO:	CATTCAATCAAGAAGAACCTGATTGGAGCTCTGCTGTTTGATTCCGGTGAAACCGCCGAG
170	GCAACACGATTGAAACGTACCGCTCGTAGGAGGTATACGCGGCGGAAAAATAGGATCTGC
	TATCTGCAGGAAATATTTAGCAACGAAATGGCCAAGGTAGACGACAGCTTCTTCCACCGG
	CTCGAGGAATCTTTCCTCGTGGAAGAAGACAAAAAGCACGAGCGCCACCCCATTTTCGGC
	AATATCGTGGACGAGGTAGCTTACCATGAAAAGTATCCAACTATTTACCACTTACGTAAG
	AAGTTAGTGGACAGCACCGATAAAGCCGACCTTCGCCTGATTTACCTAGCACTTGCACAC
	ATGATTAAGTTCCGAGGCCACTTCTTGATAGAGGGAGACCTGAATCCTGACAATTCCGAT
	GTGGATAAATTGTTCATCCAGCTGGTACAGACATACAATCAGTTGTTTGAGGAAAATCCG
	ATTAATGCCAGTGGCGTGGACGCCAAGGCTATCCTGTCTGCTCGGCTTAGTAAGAGTAGA
	CGCCTGGAAAATCTAATCGCACAGCTGCCCGGCGAAAAGAAAAATGGACTGTTCGGTAAT
	TTGATCGCCCTGAGCCTGGGCCTCACCCCTAACTTTAAGTCTAACTTCGACCTGGCCGAA
	GATGCTAAGCTCCAGCTGTCCAAAGATACTTACGATGACGATCTCGATAATCTACTGGCT
	CAGATCGGGGACCAGTACGCTGACCTGTTTCTAGCTGCCAAGAACCTCAGTGACGCCATT
	CTCCTGTCCGATATTCTGAGGGTTAACACTGAAATTACAAAGGCCCCGCTGAGCGCGAGC
	ATGATCAAAAGGTACGACGAGCATCACCAGGACCTCACGCTGCTGAAGGCCTTAGTCAGA
	CAGCAACTGCCCGAAAAGTACAAAGAAATCTTTTTCGACCAATCCAAGAACGGGTACGCC
	GGCTACATTGATGGCGGGGCTTCACAAGAGGAGTTTTACAAGTTTATCAAGCCCATCCTG
	GAGAAAATGGACGGCACTGAAGAACTGCTTGTGAAACTCAATAGGGAAGACTTACTGAGG
	AAACAGCGCACATTCGATAATGGCTCCATACCCCACCAAATCCATCTGGGAGAGTTGCAT
	GCCATCTTGCGAAGGCAGGAGGACTTCTACCCCTTTCTTAAGGACAACAGGGAGAAAATC
	GAGAAAATTCTGACTTTCCGTATCCCCTACTACGTGGGCCCACTTGCTCGCGGAAACTCA
	CGATTCGCATGGATGACCAGAAAGTCCGAGGAAACAATTACACCCTGGAATTTTGAGGAG
	GTAGTAGACAAGGGAGCCAGCGCTCAATCTTTCATTGAGAGGATGACGAATTTCGACAAG
	AACCTTCCAAACGAGAAAGTGCTTCCTAAGCACAGCCTGCTGTATGAGTATTTCACGGTG
	TACAACGAACTTACGAAGGTCAAGTATGTGACAGAGGGTATGCGGAAACCTGCTTTTCTG
	TCTGGTGAACAGAAGAAAGCTATCGTCGATCTCCTGTTTAAAACCAACCGAAAGGTGACG
	GTGAAACAGTTGAAGGAGGATTACTTCAAGAAGATCGAGTGTTTTGATTCTGTTGAAATT
	TCTGGGGTCGAGGATAGATTCAACGCCAGCCTGGGCACCTACCATGATTTGCTGAAGATT
	ATCAAGGATAAGGATTTTCTGGATAATGAGGAGAATGAAGACATTTTGGAGGATATAGTG
	CTGACCCTCACCCTGTTCGAGGACCGGGAGATGATCGAGGAGAGACTGAAAACATACGCT
	CACCTGTTTGACGACAAGGTCATGAAGCAGCTTAAGAGACGCCGTTACACAGGCTGGGGA
	AGATTATCCCGCAAATTAATCAACGGGATACGCGATAAACAAAGTGGCAAGACCATACTC
	GACTTCCTAAAGAGCGATGGATTCGCAAATCGCAATTTCATGCAGTTGATCCACGACGAT
	AGCCTGACCTTCAAAGAGGACATTCAGAAAGCGCAGGTGAGTGGTCAAGGGGATTCCCTG
	CACGAACACATTGCTAACTTGGCTGGATCACCAGCCATTAAGAAAGGCATACTGCAGACC
	GTTAAAGTGGTAGATGAGCTTGTGAAAGTCATGGGAAGACATAAGCCAGAGAACATAGTG
	ATCGAAATGGCCAGGGAAAATCAGACCACGCAAAAGGGGCAGAAGAACTCAAGAGAGCGT
	ATGAAGAGGATCGAGGAGGGCATCAAGGAGCTGGGTAGCCAGATCCTTAAAGAGCACCCA
	GTTGAGAATACCCAGCTGCAGAATGAGAAACTTTATCTCTATTATCTCCAGAACGGAAGG
	GATATGTATGTCGACCAGGAACTGGACATCAATCGGCTGAGTGATTATGACGTCGACCAC
	ATTGTGCCTCAAAGCTTTCTGAAGGATGATTCCATCGACAATAAAGTTCTGACCCGGTCT
	GATAAAAATAGAGGCAAATCCGACAACGTACCTAGCGAAGAAGTCGTCAAAAAAATGAAG
	AACTATTGGAGGCAGTTGCTGAATGCCAAGCTGATTACACAACGCAAGTTTGACAATCTC
	ACCAAGGCAGAAAGGGGGGGCCTGTCAGAACTCGACAAAGCAGGTTTCATTAAAAGGCAG
	CTAGTTGAAACTAGGCAGATTACTAAGCACGTGGCCCAGATCCTCGACTCACGGATGAAT
	ACAAAGTATGATGAGAATGATAAGCTAATCCGGGAGGTGAAGGTGATTACTCTGAAATCT
	AAGCTGGTGTCAGATTTCAGAAAAGACTTCCAGTTCTACAAAGTCAGAGAGATCAACAAT
	TATCACCATGCCCACGATGCATATCTTAATGCAGTAGTGGGGACAGCTCTGATCAAAAAA
	TATCCTAAACTGGAGTCTGAATTCGTTTATGGTGACTATAAAGTCTATGACGTCAGAAAA
	ATGATCGCAAAGAGCGAGCAGGAGATAGGGAAGGCCACAGCAAAGTACTTCTTTTACAGT
	AATATCATGAACTTTTTCAAAACTGAGATTACATTGGCTAACGGCGAGATCCGCAAGCGG
	CCACTGATAGAGACTAACGGAGAGACAGGGGAGATTGTTTGGGATAAGGGCCGTGACTTC
	GCCACCGTTAGGAAAGTGCTGTCCATGCCCCAGGTGAACATTGTGAAGAAGACAGAAGTG
	CAGACGGGTGGGTTCTCAAAAGAGTCTATTCTGCCTAAGCGGAATAGTGACAAACTGATC
	GCACGTAAAAAGGACTGGGATCCAAAAAAGTACGGCGGATTCGACAGTCCTACCGTTGCA
	TATTCCGTGCTTGTGGTCGCTAAGGTGGAGAAGGGAAAAAGCAAGAAACTGAAGTCAGTC
	AAAGAACTACTGGGCATAACGATCATGGAGCGCTCCAGTTTCGAAAAAAACCCAATCGAT
	TTTCTTGAAGCCAAGGGATACAAGGAGGTAAAGAAAGACCTTATCATTAAGCTGCCTAAG
	TACAGTCTGTTCGAACTGGAGAATGGGAGGAAGCGCATGCTGGCATCAGCTGGAGAACTC
	CAAAAAGGGAACGAGTTGGCCCTCCCCTCAAAGTATGTCAATTTTCTCTACCTGGCTTCT
	CACTACGAGAAGTTAAAGGGGTCTCCAGAGGATAATGAGCAGAAACAGCTGTTTGTGGAA
	CAGCACAAGCACTATTTGGACGAAATCATCGAACAAATTTCCGAGTTCAGTAAGAGGGTG
	ATTCTGGCCGACGCAAACCTTGACAAAGTTCTGTCCGCATACAATAAGCACAGAGACAAA
	CCAATCCGCGAGCAAGCCGAGAATATAATTCACCTTTTCACTCTGACTAATCTGGGGGCC
	CCCGCAGCATTTAAATATTTCGATACAACAATCGACCGGAAGCGGTATACATCTACTAAG
	GAAGTCCTCGATGCGACACTGATCCACCAGTCAATTACAGGTTTATATGAAACAAGAATC
	GACCTGTCCCAGCTGGGCGGCGACTAG

SEQ	AAAATTCcatGCAAAATGCTCCGGTTTCATGTCATCAAAATGATGACGTAATTAAGCATT
ID	GATAATTGAGATCCCTCTCCCTGACAGGATGATTACATAAATAATAGTGACAAAAATAAA
NO:	TTATTTATTTATCCAGAAAATGAATTGGAAAATCAGGAGAGCGTTTTCAATCCTACCTCT
171	GGCGCAGTTGATATGTcaaaCAGGTtgccgtcactgcgtcttttactggctcttctcgct
	aaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccat
	gacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
	gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctac
	ctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcac
	cgcctatctcgtgtgagataggcggagatacgaactttaagAAGGAGatataccATGGAA
	CAGGAATATTATCTGGGCTTGGACATGGGCACCGGTTCCGTCGGCTGGGCTGTTACTGAC
	AGTGAATATCACGTTCTAAGAAAGCATGGTAAGGCATTGTGGGGTGTAAGACTTTTCGAA
	TCTGCTTCCACTGCTGAAGAGCGTAGAATGTTTAGAACGAGTCGACGTAGGCTAGACAGG
	CGCAATTGGAGAATCGAAATTTTACAAGAAATTTTTGCGGAAGAGATATCTAAGAAAGAC
	CCAGGCTTTTTCCTGAGAATGAAGGAATCTAAGTATTACCCTGAGGATAAAAGAGATATA
	AATGGTAACTGTCCCGAATTGCCTTACGCATTATTTGTGGACGATGATTTTACCGATAAG
	GATTACCATAAAAAGTTCCCAACTATCTACCATTTACGCAAAATGTTAATGAATACAGAG
	GAAACCCCAGACATAAGACTAGTTTATCTGGCAATACACCATATGATGAAACATAGAGGC
	CATTTCTTACTTTCCGGGGATATCAACGAAATCAAAGAGTTTGGTACCACATTTAGTAAG
	TTACTGGAAAACATAAAGAATGAAGAATTGGATTGGAACTTAGAACTCGGAAAAGAAGAA
	TACGCGGTTGTCGAATCTATCCTGAAGGATAATATGCTGAATAGGTCGACCAAAAAAACT
	AGGCTGATCAAAGCACTGAAAGCCAAATCTATCTGCGAAAAAGCTGTTTTAAATTTACTT
	GCTGGTGGCACTGTTAAGTTATCAGACATTTTTGGTTTGGAAGAATTGAACGAAACCGAG
	CGTCCAAAAATTAGTTTCGCTGATAATGGCTACGATGATTACATTGGTGAGGTGGAAAAC
	GAGTTGGGCGAACAATTTTATATTATAGAGACAGCTAAGGCAGTCTATGACTGGGCTGTT
	TTAGTAGAAATCCTTGGTAAATACACATCTATCTCCGAAGCGAAAGTTGCTACTTACGAA
	AAGCACAAGTCCGATCTCCAGTTTTTGAAGAAAATTGTCAGGAAATATCTGACTAAGGAA
	GAATATAAAGATATTTTCGTTAGTACCTCTGACAAACTGAAAAATTACTCCGCTTACATC
	GGGATGACCAAGATTAATGGCAAAAAAGTTGATCTGCAAAGCAAAAGGTGTTCGAAGGAA
	GAATTTTATGATTTCATTAAAAAGAATGTCTTAAAAAAATTAGAAGGTCAGCCAGAATAC
	GAATATTTGAAAGAAGAACTGGAAAGAGAGACATTCTTACCAAAACAAGTCAACAGAGAT
	AATGGGGTAATTCCATATCAAATTCACCTCTACGAATTAAAAAAAATTTTAGGCAATTTA
	CGCGATAAAATTGACCTTATCAAAGAAAATGAGGATAAGCTGGTTCAACTCTTTGAATTC
	AGAATACCCTATTATGTGGGCCCACTGAACAAGATTGATGACGGCAAAGAAGGTAAATTC
	ACATGGGCCGTCCGCAAATCCAATGAAAAAATTTACCCATGGAACTTTGAAAATGTAGTA
	GATATTGAAGCGTCTGCGGAGAAATTTATTCGAAGAATGACTAATAAATGCACTTACTTG
	ATGGGAGAGGATGTTCTGCCTAAAGACAGCTTATTATACAGCAAGTACATGGTTCTAAAC
	GAACTTAACAACGTTAAGTTGGACGGTGAGAAATTAAGTGTAGAATTGAAACAAAGATTG
	TATACTGACGTCTTCTGCAAGTACAGAAAAGTGACAGTTAAAAAAATTAAGAATTACTTG
	AAGTGCGAAGGTATAATTTCTGGAAACGTAGAGATTACTGGTATTGATGGTGATTTCAAA
	GCATCCCTAACAGCTTACCACGATTTCAAGGAAATCCTGACAGGAACTGAACTCGCAAAA
	AAAGATAAAGAAAACATTATTACTAATATTGTTCTTTTCGGTGATGACAAGAAATTGTTG
	AAGAAAAGACTGAATAGACTTTACCCCCAGATTACTCCCAATCAACTTAAGAAAATTTGT
	GCTTTGTCTTACACAGGATGGGGTCGTTTTTCAAAAAAGTTCTTAGAAGAGATTACCGCA
	CCTGATCCAGAAACAGGCGAAGTATGGAATATAATTACCGCCTTATGGGAATCGAACAAT
	AATCTTATGCAACTTCTGAGCAATGAATATCGTTTCATGGAAGAAGTTGAGACTTACAAC
	ATGGGCAAACAGACGAAGACTTTATCCTATGAAACTGTGGAAAATATGTATGTATCACCT
	TCTGTCAAGAGACAAATTTGGCAAACCTTAAAAATTGTCAAAGAATTAGAAAAGGTAATG
	AAGGAGTCTCCTAAACGTGTGTTTATTGAAATGGCTAGAGAAAAACAAGAGTCAAAAAGA
	ACCGAGTCAAGAAAGAAGCAGTTAATCGATTTATATAAGGCTTGTAAAAACGAAGAGAAA
	GATTGGGTTAAAGAATTGGGGGACCAAGAGGAACAAAAACTACGGTCGGATAAGTTGTAT
	TTATACTATACGCAAAAGGGACGATGTATGTATTCCGGCGAGGTAATAGAATTGAAGGAT
	TTATGGGACAATACAAAATATGACATAGACCATATATATCCCCAATCAAAAACGATGGAC
	GATAGCTTGAACAATAGAGTACTCGTGAAAAAAAAATATAATGCGACCAAATCTGATAAG
	TATCCTCTGAATGAAAATATCAGACATGAAAGAAAGGGGTTCTGGAAGTCCTTGTTAGAT
	GGTGGGTTTATAAGCAAAGAAAAGTACGAGCGTCTAATAAGAAACACGGAGTTATCGCCA
	GAAGAACTCGCTGGTTTTATTGAGAGGCAAATCGTGGAAACGAGACAATCTACCAAAGCC
	GTTGCTGAGATCCTAAAGCAAGTTTTCCCAGAGTCGGAGATTGTCTATGTCAAAGCTGGC
	ACAGTGAGCAGGTTTAGGAAAGACTTCGAACTATTAAAGGTAAGAGAAGTGAACGATTTA
	CATCACGCAAAGGACGCTTACCTAAATATCGTTGTAGGTAACTCATATTATGTTAAATTT
	ACCAAGAACGCCTCTTGGTTTATAAAGGAGAACCCAGGTAGAACATATAACCTGAAAAAG
	ATGTTCACCTCTGGTTGGAATATTGAGAGAAACGGAGAAGTCGCATGGGAAGTTGGTAAG
	AAAGGGACTATAGTGACAGTAAAGCAAATTATGAACAAAAATAATATCCTCGTTACAAGG
	CAGGTTCATGAAGCAAAGGGCGGCCTTTTTGACCAACAAATTATGAAGAAAGGGAAAGGT
	CAAATTGCAATAAAAGAAACCGATGAGAGACTAGCGTCAATAGAAAAGTATGGTGGCTAT
	AATAAAGCTGCGGGTGCATACTTTATGCTTGTTGAATCAAAAGACAAGAAAGGTAAGACT
	ATTAGAACTATAGAATTTATACCCCTGTACCTTAAAAACAAAATTGAATCGGATGAGTCA
	ATCGCGTTAAATTTTCTAGAGAAAGGAAGGGGTTTAAAAGAACCAAAGATCCTGTTAAAA
	AAGATTAAGATTGACACCTTGTTCGATGTAGATGGATTTAAAATGTGGTTATCTGGCAGA
	ACAGGCGATAGACTTTTGTTTAAGTGCGCTAATCAATTAATTTTGGATGAGAAAATCATT
	GTCACAATGAAAAAAATAGTTAAGTTTATTCAGAGAAGACAAGAAAACAGGGAGTTGAAA
	TTATCTGATAAAGATGGTATCGACAATGAAGTTTTAATGGAAATCTACAATACATTCGTT
	GATAAACTTGAAAATACCGTATATCGAATCAGGTTAAGTGAACAAGCCAAAACATTAATT
	GATAAACAAAAAGAATTTGAAAGGCTATCACTGGAAGACAAATCCTCCACCCTATTTGAA
	ATTTTGCATATATTCCAGTGCCAATCTTCAGCAGCTAATTTAAAAATGATTGGCGGACCT
	GGGAAAGCCGGCATCCTAGTGATGAACAATAATATCTCCAAGTGTAACAAAATATCAATT
	ATTAACCAATCTCCGACAGGTATTTTTGAAAATGAAATAGACTTGCTTAAGATATAAGAA
	ATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTTATTATATCGCGTTGATT
	ATTGATGCTGTTTTTAGTTTTAACGGCAATTAATATATGTGTTATTAATTGAATGAATTT
	TATCATTCATAATAAGTATGTGTAGGATCAAGCTCAGGTTAAATATTCACTCAGGAAGTT
	ATTACTCAGGAAGCAAAGAGGATTACAGAATTATCTCATAACAAGTGTTAAGGGATGTTA
	TTTCC

SEQ	AATTCAAAGGATAATCAAAC
ID
NO:
172

SEQ	AATCTCTACTCTTTGTAGAT
ID
NO:
173

SEQ	AATTTCTACTGTTGTAGAT
ID
NO:
174

SEQ	AATTTCTACTAGTGTAGAT
ID
NO:
175

SEQ	AATTTCTACTATTGT
ID
NO:
176

SEQ	AATTTCTACTGTTGTAGA
ID
NO:
177

SEQ	AATTTCTACTATTGTA
ID
NO:
178

SEQ	AATTTCTACTTTTGTAGAT
ID
NO:
179

SEQ	AATTTCTACTGTTGTAGAT
ID
NO:
180

SEQ	AATTTCTACTCTTGTAGAT
ID
NO:
181

Claims

What is claimed is:

1. A composition comprising:

i) a first donor nucleic acid comprising:

a) a modified first target nucleic acid sequence;

b) a first protospacer adjacent motif (PAM) mutation; and

c) a first guide nucleic acid sequence comprising a first spacer region complementary to a portion of the first target nucleic acid; and

ii) a second donor nucleic acid comprising:

a) a barcode corresponding to the modified first target nucleic acid sequence; and

b) a second guide nucleic acid sequence comprising a second spacer region complementary to a portion of a second target nucleic acid.

2. The composition of claim 1, wherein the modified first target nucleic acid sequence comprises at least one inserted, deleted, or substituted nucleic acid compared to a corresponding un-modified first target nucleic acid.

3. The composition of claim 1, wherein the first guide nucleic acid and second guide nucleic acid are compatible with a nucleic acid-guided nuclease.

4. The composition of claim 3, wherein the nucleic acid-guided nuclease is a Type II or Type V Cas protein.

5. The composition of claim 3, wherein the nucleic acid-guided nuclease is a Cas9 homologue or a Cpf1 homologue.

6. The composition of claim 1, wherein the second donor nucleic acid comprises a second PAM mutation.

7. The composition of claim 1, wherein the second donor nucleic acid sequence comprises a regulatory sequence or a mutation to turn a screenable or selectable marker on or off.

8. The composition of claim 1, wherein the second donor nucleic acid sequence targets a unique landing site.

9. A method of genome engineering, the method comprising:

a) contacting a population of cells with a polynucleotide, wherein each cell comprises a first target nucleic acid, a second target nucleic acid, and a nucleic acid-guided nuclease,

wherein the polynucleotide comprises

1) an editing cassette comprising:

i) a modified first target nucleic acid sequence;

ii) a first protospacer adjacent motif (PAM) mutation;

iii) a first guide nucleic acid sequence comprising a spacer region complementary to a portion of the first target nucleic acid and compatible with the nucleic acid-guided nuclease; and

2) a recorder cassette comprising

i) a barcode corresponding to the modified first target nucleic acid sequence; and

ii) a second guide nucleic acid sequence comprising a second spacer region complementary to a portion of the second target nucleic acid and compatible with the nucleic acid-guided nuclease;

b) allowing the first guide nucleic acid sequence, the second guide nucleic acid sequence, and the nucleic acid-guided nuclease to create a genome edit within the first target nucleic acid and the second target nucleic acid.

10. The method of claim 9, further comprising c) sequencing a portion of the barcode, thereby identifying the modified first target nucleic acid that was inserted within the first target nucleic acid in step a).

11. The method of claim 9, wherein the nucleic acid-guided nuclease is a CRISPR nuclease.

12. The method of claim 9, wherein the PAM mutation is not recognized by the nucleic acid-guided nuclease.

13. The method of claim 9, wherein the nucleic acid-guided nuclease is a Type II or Type V Cas protein.

14. The method of claim 9, wherein the nucleic acid-guided nuclease is a Cas9 homologue or a Cpf1 homologue.

15. The method of claim 9, wherein the recorder cassette further comprises a second PAM mutation that is not recognized by the nucleic acid-guided nuclease.

16. A method of selectable recursive genetic engineering comprising

a) contacting cells comprising a nucleic acid-guided nuclease with a polynucleotide comprising a recorder cassette, said recorder cassette comprising

i) a nucleic acid sequence that recombines into a unique landing site incorporated during a previous round of engineering, wherein the nucleic acid sequence comprises a unique barcode; and

ii) a guide RNA compatible with the nucleic acid-guided nuclease that targets the unique landing site; and

b) allowing the nucleic acid-guided nuclease to edit the unique landing site, thereby incorporating the unique barcode into the unique landing site.

17. The method of claim 16, wherein the nucleic acid sequence further comprises a regulatory sequence that turns transcription of a screenable or selectable marker on or off.

18. The method of claim 16, wherein the nucleic acid sequence further comprises a PAM mutation that is not compatible with the nucleic acid-guided nuclease.

19. The method of claim 16, wherein the nucleic acid sequence further comprises a second unique landing site for subsequent engineering rounds.

20. The method of claim 16, wherein the polynucleotide further comprises an editing cassette comprising

a) a modified first target nucleic acid sequence;

b) a first protospacer adjacent motif (PAM) mutation; and

c) a first guide nucleic acid sequence comprising a first spacer region complementary to a portion of the first target nucleic acid,

wherein the unique barcode corresponds to the modified first target nucleic acid such that the modified target nucleic acid can be identified by the unique barcode.