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US20220133919A1 - ONE STEP 64Cu-BaBaSar-RGD2 PRODUCTION METHOD - Google Patents

ONE STEP 64Cu-BaBaSar-RGD2 PRODUCTION METHOD Download PDF

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US20220133919A1
US20220133919A1 US17/574,963 US202217574963A US2022133919A1 US 20220133919 A1 US20220133919 A1 US 20220133919A1 US 202217574963 A US202217574963 A US 202217574963A US 2022133919 A1 US2022133919 A1 US 2022133919A1
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rgd
babasar
solution
kit
pet
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Shuanglong Liu
Peter Conti
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University of Southern California USC
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/08—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
    • A61K51/082—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins the peptide being a RGD-containing peptide
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/041—Heterocyclic compounds
    • A61K51/044—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine, rifamycins
    • A61K51/0446—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine, rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/0474—Organic compounds complexes or complex-forming compounds, i.e. wherein a radioactive metal (e.g. 111In3+) is complexed or chelated by, e.g. a N2S2, N3S, NS3, N4 chelating group
    • A61K51/0482—Organic compounds complexes or complex-forming compounds, i.e. wherein a radioactive metal (e.g. 111In3+) is complexed or chelated by, e.g. a N2S2, N3S, NS3, N4 chelating group chelates from cyclic ligands, e.g. DOTA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/0497—Organic compounds conjugates with a carrier being an organic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/08—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
    • A61K51/088—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins conjugates with carriers being peptides, polyamino acids or proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/12—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by a special physical form, e.g. emulsion, microcapsules, liposomes, characterized by a special physical form, e.g. emulsions, dispersions, microcapsules
    • A61K51/121—Solutions, i.e. homogeneous liquid formulation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/12—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by a special physical form, e.g. emulsion, microcapsules, liposomes, characterized by a special physical form, e.g. emulsions, dispersions, microcapsules
    • A61K51/1241—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by a special physical form, e.g. emulsion, microcapsules, liposomes, characterized by a special physical form, e.g. emulsions, dispersions, microcapsules particles, powders, lyophilizates, adsorbates, e.g. polymers or resins for adsorption or ion-exchange resins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2123/00—Preparations for testing in vivo

Definitions

  • the present invention relates in general to the preparation of imaging probes.
  • Tumor-induced angiogenesis plays a critical role in tumor progression and metastasis. Without new vasculature and blood circulation, tumor stops growing at the size of 1-2 mm3 and may become necrotic or even apoptotic since diffusion is already insufficient to supply the tissue with oxygen and nutrients (1, 2). Substantial efforts have been made to develop therapeutic strategies that interrupt the angiogenic process to stop the tumor growth (3). Integrin ⁇ v ⁇ 3 is a vital component for the angiogenic process by mediating endothelial cell (EC) migration and survival during angiogenesis (4). For neovasculature formation, ECs need to migrate into an avascular region and to extensively remodel the extracellular matrix (ECM).
  • ECM extracellular matrix
  • integrins ⁇ v ⁇ 3 an immunoglobulin superfamily molecule has proved to be one of the most important cell adhesion receptors for various ECM proteins. While in normal tissues, expression of integrin ⁇ v ⁇ 3 is much lower, making integrin ⁇ v ⁇ 3 an ideal target for diagnosis and therapy in cancer study.
  • a protocol to non-invasively quantify its expression levels will provide a method to document integrin levels, which can support the anti-integrin ⁇ v ⁇ 3 treatment for the patients, and effectively monitor treatment progress for the integrin ⁇ v ⁇ 3-positive patients.
  • Non-invasive detection and quantification of integrin ⁇ v ⁇ 3 is also leading to the diagnosis of many types of cancer at their earliest stages (5).
  • Peptides containing Arg-Glu-Asp (RGD) amino acid sequence have a high binding affinity and selectivity for integrin ⁇ v ⁇ 3 (6).
  • RGD Arg-Glu-Asp
  • a number of peptides containing RGD sequences have been developed to target tumors overexpressing ⁇ v ⁇ 3 receptors (7).
  • RGD peptides have been modified and radiolabeled for positron emission tomography (PET) probe development.
  • PET positron emission tomography
  • 18 F-galacto-RGD With conjugation of a sugar moiety for reducing the liver uptake, 18 F-galacto-RGD is still specifically binding to integrin ⁇ v ⁇ 3 , shows a more desirable biodistribution in humans, and provides a better visualization of ⁇ v ⁇ 3 expression in tumors with high contrast (8).
  • a major disadvantage for 18 F-galacto-RGD is the long and sophisticated preparation, including multiple synthetic steps that complicate routine production (9).
  • the low ⁇ + energy of 64 Cu gives a resolution down to 1 mm in PET images and is important to achieve lower radiation doses for the patients (21).
  • Cage-like hexaazamacrobicyclic sarcophagine chelator completely encapsulates the coordinated Cu 2+ ions. Their complexes exhibit enhanced thermodynamic and kinetic stability to copper-binding proteins in vivo (22).
  • a BaBaSar chelator for conjugation with RGD peptide (BaBaSar-RGD 2 ) was developed.
  • the 64 Cu labeling chemistry for BaBaSar-RGD 2 was achieved at room temperature to give a quantitative yield.
  • the resulting 64 Cu-BaBaSar-RGD 2 probe shows great stability both in vitro and in vivo, providing high tumor uptake and low normal organ uptake in U87MG glioblastoma tumor bearing mice (23). Due to the wide application of RGD peptide in diagnostic and therapeutic applications, there is a need for PET radiotracer for integrin imaging that can be made easily. Such a PET radiotracer would be of great interest to both radiochemists and physicians.
  • One aspect of the present invention is directed to a method of preparing a 64 Cu-BaBaSar-RGD 2 solution.
  • the method includes lyophilizing a solution of BaBaSar-RGD 2 and adding a 64 Cu solution to the lyophilized BaBaSar-RGD 2 .
  • the 64 Cu solution includes 64 CuCl 2 .
  • the 64 Cu solution includes buffer salts.
  • the solution of BaBaSar-RGD 2 includes buffer salts.
  • the buffer salts include sodium acetate buffer.
  • Another aspect of the present invention is directed to a method preparing a 64 Cu-BaBaSar-RGD 2 solution.
  • the method includes lyophilizing a 64 Cu-BaBaSar-RGD 2 solution and reconstituting the 64 Cu-BaBaSar-RGD 2 solution with an aqueous solution.
  • the 64 Cu-BaBaSar-RGD 2 solution includes buffer salts.
  • the buffer salts include sodium acetate buffer.
  • kits for preparing a positron emission tomography (PET) probe includes a lyophilized powder of BaBaSar-RGD 2 and instructions on how to reconstitute the lyophilized powder with a 64 Cu solution.
  • the 64 Cu solution comprises a 64 Cu 2+ salt dissolved or dispersed in a suitable liquid medium.
  • the 64 Cu solution comprises a 64 Cu halide salt, wherein the halide is selected from a group that includes fluorine, chlorine, bromine and iodine.
  • the 64 Cu solution can include one or more buffer salts.
  • the solution of BaBaSar-RGD 2 can include one or more buffer salts.
  • FIG. 1 Kit production process of 64 Cu-BaBaSar-RGD 2 .
  • FIG. 2 Structure of RGD peptide and the synthesis route for 64 Cu-BaBaSar-RGD 2 .
  • FIG. 3 Analytical radio trace HPLC chromatogram for the purity of the 64 Cu-BaBaSar-RGD 2 .
  • FIG. 4 Decay-corrected anterior maximum-intensity projections of PET/CT at 1, 5, 10, 20, 40, 60, 120, and 180 min after injection of 64 Cu-BaBaSar-RGD 2 in macaque monkey.
  • FIG. 5 Structures of AnAnSar, BaAnSar, BaMalSar, and MalMalSar.
  • FIGS. 6-7 Additional peptides to be used in present invention.
  • the present invention provides a straightforward, one-step synthesis of 64 Cu-BaBaSar-RGD 2 radiopharmaceutical using a preloaded cold kit.
  • the present invention provides a one-step production of radiopharmaceutical 64 Cu-BaBaSar-RGD 2 with a kit preloaded with all the precursors.
  • FIG. 1 discloses the process of production. Furthermore, this method is not limited to the production of 64 Cu-BaBaSar-RGD 2 .
  • the same kit method associated with BaBaSar chelator could be used too. For example, other peptides can be used in place of RGD.
  • this kit method provides a universal method for 64 Cu radiopharmaceutical production.
  • BaBaSar-RGD 2 was synthesized as previously reported (23). All commercial chemicals were of analytic grade and used without further purification. 64 Cu in hydrochloric acid was obtained from Washington University (St. Louis, Mo.) or produced in the Molecular Imaging Center Cyclotron Facility. Analytic reversed-phase high-performance liquid chromatography (RP-HPLC) using a Phenomenex Luna column (5 ⁇ , C 18 , 250 ⁇ 4.6 mm) were performed on a Dionex U3000 chromatography system with a diode arrays detector and radioactivity flow-count (Eckert & Ziegler, Valencia, Calif.). The recorded data were processed using Chromeleon version 7.20 software. The flow rate of analytical HPLC was 1.0 mL/min.
  • the mobile phase starts from 95% solvent A (0.1% trifluoroacetic acid [TFA] in water) and 5% solvent B (0.1% TFA in acetonitrile [MeCN]). From 2 to 32 min, the mobile phase ramped to 35% solvent A and 65% solvent B.
  • the ultraviolet (UV) detector of HPLC was set at 254 nm.
  • the endotoxin analysis was performed on a portable Endosafe®-PTSTM system consisting of LAL reagent and endotoxin controls applied to a single use, polystyrene cartridge.
  • the 18.2 M ⁇ cm water from in-house GenPureTM station was treated with chelex 100 resin 48 hours before use. All the solution hereafter was prepared with this treated water.
  • the lyophilized BaBaSar-RGD 2 (1.0 mg) was dissolved in 1.0 mL sodium acetate buffer (NaOAc, 0.1 M, pH 5.5). The pH of BaBaSar-RGD 2 solution was adjusted to pH 5.5 using 0.1 M sodium hydroxide (NaOH). Then, the BaBaSar-RGD 2 solution was equally aliquoted to 20 Eppendorf vials (1.5 mL). The filled vials were frozen using dry ice and then transferred to the bottles of the Labconco Freeze Dry System (pressure ⁇ 100 mTorr). After the solvent was removed, the vials containing BaBaSar-RGD 2 powder were then sealed and stored at ⁇ 18° C. for 64 Cu labeling.
  • 64CuCl2 (5-30 mCi) purchased from Washington University at St. Louis was reconstituted using 200-300 ⁇ L NaOAc buffer (0.1 M, pH 5.5) and added to a vial prepared in above section. The vial was gently shaking at room temperature for 5 min. After the reaction was quenched with 5.0 mL saline, the activity passed through a 0.22 ⁇ m sterile filter (Pall Corp.) into a 10 mL Allergy vial for quality control test and animal/human injection.
  • NaOAc buffer 0.1 M, pH 5.5
  • a cold kit can contain 50 ⁇ g BaBaSar-RGD 2 ligand to which 64 CuCl 2 is to be complexed, and buffer salts to adjust the pH suitable for the labelling conditions.
  • the kits are prepared in a lyophilized form and have a long shelf life of over 3 months at room temperature. When the cold kits are stored in a refrigerator at 2-8° C., the shelf life is over a year.
  • the labeling chemistry for 64 Cu-BaBaSar-RGD 2 is disclosed in FIG. 2 .
  • the 64 Cu-labeling yield for 64 Cu-BaBaSar-RGD 2 was >99% based on HPLC analysis ( FIG. 3 ).
  • approximately 15-20% 64 Cu-BaBaSar-RGD 2 was trapped onto the filter and the overall recovered yield for 64 Cu-BaBaSar-RGD 2 is about 80% calculated from the loaded 64 Cu.
  • the radiochemical purity of 64 Cu-BaBaSar-RGD 2 was >99% based on radiotrace analytical HPLC ( FIG. 3 ).
  • the injection of 13.1-19.7 MBq/kg of 64 Cu-BaBaSar-RGD 2 in macaque monkey produced no observable effects on vital signs (blood pressure, pulse, and electrocardiogram) during and 24-h after PET scan.
  • the PET images at 1, 5, 10, 20, 40, 60, 120, and 180 min after injection are disclosed in FIG. 4 .
  • the bladder content was visualized at 10 min after injection and more and more activity was accumulated in urine bladder content.
  • the bladder did not void because the macaque monkey was under anesthesia.
  • Gallbladder uptake was not observed during the whole scan. Rapid clearance of activity in the liver was observed in the images at time points after 1 min.
  • the urinary bladder had the highest uptake, with 51.37% ⁇ 8.73% of injected activity at 1 h post injection.
  • the maximum uptake for the liver, and kidneys were 37.40 ⁇ 6.63% ID (9 min) and 26.79 ⁇ 4.35% ID (0.5 min) respectively.
  • 8.62% ⁇ 1.41% of injected activity was found in the gallbladder, small intestine, and upper and lower portions of the large intestine.
  • the mean organ doses for the male human phantom were calculated with Olinda/EXM using 64 Cu-BaBaSar-RGD 2 biodistribution in monkey (Table 2).
  • the kidneys had the highest radiation-absorbed doses (108.43 ⁇ Gy/MBq), followed by the bladder wall (87.07 ⁇ Gy/MBq).
  • the mean effective dose of 64 Cu-BaBaSar-RGD 2 was 15.30 ⁇ 2.21 ⁇ Sv/MBq.
  • the effective dose for the non-voiding model is estimated to be 14.2 mSv, which is comparable to the estimated 6.23 mSv dose in a whole-body PET scan with 2-deoxy-2-[ 18 F]fluoro-D-glucose ( 18 F-FDG) (24).
  • the estimated doses for the female human were higher by 18% because body and organ sizes of women are smaller than those men (data not shown).
  • Integrin ⁇ v ⁇ 3-targeted radiopharmaceutical 64 Cu-BaBaSar-RGD 2 has been successfully synthesized with the one-step kit method.
  • the straightforward method greatly simplifies the production process and benefits the clinical application of 64 Cu-BaBaSar-RGD 2 .
  • Human radiation dosimetry of 64 Cu-BaBaSar-RGD 2 was estimated after intravenous administration in macaque monkey, by PET imaging and OLINDA/EXM calculations.
  • the critical organs were kidneys and urinary bladder wall.
  • the mean effective dose, determined with the male adult model, was 15.30 ⁇ 2.21 ⁇ Sv/MBq. This PET probe demonstrates an acceptable radiation dose comparable to other reported RGD-derived radiopharmaceuticals.
  • 64 Cu-BaBaSar-RGD 2 as an integrin marker, with a desirable biodistribution and safety characteristics in monkey. Therefore, 64 Cu-BaBaSar-RGD 2 can safely be used in human scan for further evaluation of its performance as an integrin-targeting probe.

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Abstract

A method of preparing a 64Cu-BaBaSar-RGD2 solution is provided. The method includes lyophilizing a solution of BaBaSar-RGD2 and adding a 64Cu solution to the lyophilized BaBaSar-RGD2.

Description

    CROSS-REFERENCE TO RELATED APPLICATIONS
  • This application is a divisional application of U.S. application Ser. No. 16/205,985 filed Nov. 30, 2018, now pending; which claims the benefit under 35 USC § 119(e) to U.S. Application Ser. No. 62/593,723 filed Dec. 1, 2017, now expired. The disclosure of each of the prior applications is considered part of and is incorporated by reference in the disclosure of this application.
    • FIELD OF THE INVENTION
  • The present invention relates in general to the preparation of imaging probes.
    • BACKGROUND OF THE INVENTION
  • Tumor-induced angiogenesis plays a critical role in tumor progression and metastasis. Without new vasculature and blood circulation, tumor stops growing at the size of 1-2 mm3 and may become necrotic or even apoptotic since diffusion is already insufficient to supply the tissue with oxygen and nutrients (1, 2). Substantial efforts have been made to develop therapeutic strategies that interrupt the angiogenic process to stop the tumor growth (3). Integrin αvβ3 is a vital component for the angiogenic process by mediating endothelial cell (EC) migration and survival during angiogenesis (4). For neovasculature formation, ECs need to migrate into an avascular region and to extensively remodel the extracellular matrix (ECM). In this process, integrins αvβ3, an immunoglobulin superfamily molecule has proved to be one of the most important cell adhesion receptors for various ECM proteins. While in normal tissues, expression of integrin αvβ3 is much lower, making integrin αvβ3 an ideal target for diagnosis and therapy in cancer study. A protocol to non-invasively quantify its expression levels will provide a method to document integrin levels, which can support the anti-integrin αvβ3 treatment for the patients, and effectively monitor treatment progress for the integrin αvβ3-positive patients. Non-invasive detection and quantification of integrin αvβ3 is also leading to the diagnosis of many types of cancer at their earliest stages (5).
  • Peptides containing Arg-Glu-Asp (RGD) amino acid sequence have a high binding affinity and selectivity for integrin αvβ3 (6). In the last two decades, a number of peptides containing RGD sequences have been developed to target tumors overexpressing αvβ3 receptors (7). RGD peptides have been modified and radiolabeled for positron emission tomography (PET) probe development. 18F-galacto-RGD is the first RGD probe tested in human subjects for detecting αvβ3 expression. With conjugation of a sugar moiety for reducing the liver uptake, 18F-galacto-RGD is still specifically binding to integrin αvβ3, shows a more desirable biodistribution in humans, and provides a better visualization of αvβ3 expression in tumors with high contrast (8). However, a major disadvantage for 18F-galacto-RGD is the long and sophisticated preparation, including multiple synthetic steps that complicate routine production (9). Due to the importance of RGD peptides, continued efforts have been made to achieve desirable PET probes for easy production, optimal pharmacokinetics, and higher tumor uptake, such as 18F-AH111585 (10-12), 18F-alfatide (13,14), 18F-RGD-K5 (15,16), 18F-FPPRGD2 (17,18), 18F-fluciclatide (12,19), and 68Ga-NOTA-PRGD2 (20).
  • 64Cu (T1/2 =12.7 h; β+ 0.653 MeV [17.8%]) has been widely used for radiolabeling proteins, antibodies and peptides for PET probe development. The low β+ energy of 64Cu gives a resolution down to 1 mm in PET images and is important to achieve lower radiation doses for the patients (21). Cage-like hexaazamacrobicyclic sarcophagine chelator completely encapsulates the coordinated Cu2+ ions. Their complexes exhibit enhanced thermodynamic and kinetic stability to copper-binding proteins in vivo (22). Starting from hexaazamacrobicyclic sarcophagine, a BaBaSar chelator for conjugation with RGD peptide (BaBaSar-RGD2) was developed. The 64Cu labeling chemistry for BaBaSar-RGD2 was achieved at room temperature to give a quantitative yield. The resulting 64Cu-BaBaSar-RGD2 probe shows great stability both in vitro and in vivo, providing high tumor uptake and low normal organ uptake in U87MG glioblastoma tumor bearing mice (23). Due to the wide application of RGD peptide in diagnostic and therapeutic applications, there is a need for PET radiotracer for integrin imaging that can be made easily. Such a PET radiotracer would be of great interest to both radiochemists and physicians.
    • SUMMARY OF THE INVENTION
  • One aspect of the present invention is directed to a method of preparing a 64Cu-BaBaSar-RGD2 solution. The method includes lyophilizing a solution of BaBaSar-RGD2 and adding a 64Cu solution to the lyophilized BaBaSar-RGD2.
  • In one embodiment, the 64Cu solution includes 64CuCl2.
  • In another embodiment, the 64Cu solution includes buffer salts.
  • In another embodiment, the solution of BaBaSar-RGD2 includes buffer salts.
  • In another embodiment, the buffer salts include sodium acetate buffer.
  • Another aspect of the present invention is directed to a method preparing a 64Cu-BaBaSar-RGD2 solution. The method includes lyophilizing a 64Cu-BaBaSar-RGD2 solution and reconstituting the 64Cu-BaBaSar-RGD2 solution with an aqueous solution.
  • In one embodiment, the 64Cu-BaBaSar-RGD2 solution includes buffer salts.
  • In another embodiment, the buffer salts include sodium acetate buffer.
  • Another aspect of the present invention is to provide a kit for preparing a positron emission tomography (PET) probe. The kit includes a lyophilized powder of BaBaSar-RGD2 and instructions on how to reconstitute the lyophilized powder with a 64Cu solution.
  • In some embodiments, the 64Cu solution comprises a 64Cu2+ salt dissolved or dispersed in a suitable liquid medium. The 64Cu solution comprises a 64Cu halide salt, wherein the halide is selected from a group that includes fluorine, chlorine, bromine and iodine.
  • In some embodiments, the 64Cu solution can include one or more buffer salts.
  • In some embodiments, the solution of BaBaSar-RGD2 can include one or more buffer salts.
  • Other aspects and advantages of the invention will be apparent from the following description and the appended claims.
    • BRIEF DESCRIPTION OF THE DRAWINGS
  • FIG. 1: Kit production process of 64Cu-BaBaSar-RGD2.
  • FIG. 2: Structure of RGD peptide and the synthesis route for 64Cu-BaBaSar-RGD2.
  • FIG. 3: Analytical radio trace HPLC chromatogram for the purity of the 64Cu-BaBaSar-RGD2.
  • FIG. 4: Decay-corrected anterior maximum-intensity projections of PET/CT at 1, 5, 10, 20, 40, 60, 120, and 180 min after injection of 64Cu-BaBaSar-RGD2 in macaque monkey.
  • FIG. 5: Structures of AnAnSar, BaAnSar, BaMalSar, and MalMalSar.
  • FIGS. 6-7: Additional peptides to be used in present invention.
    •  DETAILED DESCRIPTION OF THE INVENTION
  • Unless otherwise indicated herein, all terms used herein have the meanings that the terms would have to those skilled in the art of the present invention. Practitioners are particularly directed to current textbooks for definitions and terms of the art. It is to be understood, however, that this invention is not limited to the particular methodology, protocols, and reagents described, as these may vary.
  • To promote the clinical application of 64Cu-BaBaSar-RGD2 in humans, the present invention provides a straightforward, one-step synthesis of 64Cu-BaBaSar-RGD2 radiopharmaceutical using a preloaded cold kit.
  • The present invention provides a one-step production of radiopharmaceutical 64Cu-BaBaSar-RGD2 with a kit preloaded with all the precursors. FIG. 1 discloses the process of production. Furthermore, this method is not limited to the production of 64Cu-BaBaSar-RGD2. When biological ligands other than RGD peptides are conjugated with the BaBaSar chelator, the same kit method associated with BaBaSar chelator could be used too. For example, other peptides can be used in place of RGD. In addition, other chelators, preferably sarcophagine based chelators, such as AnAnSar, BaAnSar, BaMalSar, and MalMalSar can be used in place of BaBaSar. Therefore, this kit method provides a universal method for 64Cu radiopharmaceutical production.
    • General Method
  • BaBaSar-RGD2 was synthesized as previously reported (23). All commercial chemicals were of analytic grade and used without further purification. 64Cu in hydrochloric acid was obtained from Washington University (St. Louis, Mo.) or produced in the Molecular Imaging Center Cyclotron Facility. Analytic reversed-phase high-performance liquid chromatography (RP-HPLC) using a Phenomenex Luna column (5μ, C18, 250×4.6 mm) were performed on a Dionex U3000 chromatography system with a diode arrays detector and radioactivity flow-count (Eckert & Ziegler, Valencia, Calif.). The recorded data were processed using Chromeleon version 7.20 software. The flow rate of analytical HPLC was 1.0 mL/min. The mobile phase starts from 95% solvent A (0.1% trifluoroacetic acid [TFA] in water) and 5% solvent B (0.1% TFA in acetonitrile [MeCN]). From 2 to 32 min, the mobile phase ramped to 35% solvent A and 65% solvent B. The ultraviolet (UV) detector of HPLC was set at 254 nm. The endotoxin analysis was performed on a portable Endosafe®-PTS™ system consisting of LAL reagent and endotoxin controls applied to a single use, polystyrene cartridge.
    • Radiopharmaceutical Preparation
  • Preparation of 64Cu-BaBaSar-RGD2 Production Kit
  • The 18.2 MΩ·cm water from in-house GenPure™ station was treated with chelex 100 resin 48 hours before use. All the solution hereafter was prepared with this treated water. The lyophilized BaBaSar-RGD2 (1.0 mg) was dissolved in 1.0 mL sodium acetate buffer (NaOAc, 0.1 M, pH 5.5). The pH of BaBaSar-RGD2 solution was adjusted to pH 5.5 using 0.1 M sodium hydroxide (NaOH). Then, the BaBaSar-RGD2 solution was equally aliquoted to 20 Eppendorf vials (1.5 mL). The filled vials were frozen using dry ice and then transferred to the bottles of the Labconco Freeze Dry System (pressure<100 mTorr). After the solvent was removed, the vials containing BaBaSar-RGD2 powder were then sealed and stored at −18° C. for 64Cu labeling.
    • 64Cu-Labeling Chemistry
  • 64CuCl2 (5-30 mCi) purchased from Washington University at St. Louis was reconstituted using 200-300 μL NaOAc buffer (0.1 M, pH 5.5) and added to a vial prepared in above section. The vial was gently shaking at room temperature for 5 min. After the reaction was quenched with 5.0 mL saline, the activity passed through a 0.22 μm sterile filter (Pall Corp.) into a 10 mL Allergy vial for quality control test and animal/human injection.
    • Kit Preparation
  • A cold kit can contain 50 μg BaBaSar-RGD2 ligand to which 64CuCl2 is to be complexed, and buffer salts to adjust the pH suitable for the labelling conditions. The kits are prepared in a lyophilized form and have a long shelf life of over 3 months at room temperature. When the cold kits are stored in a refrigerator at 2-8° C., the shelf life is over a year.
    • Radiochemistry
  • The labeling chemistry for 64Cu-BaBaSar-RGD2 is disclosed in FIG. 2. The 64Cu-labeling yield for 64Cu-BaBaSar-RGD2 was >99% based on HPLC analysis (FIG. 3). However, after passing through 0.22 μm Pall filter to remove pyrogen, approximately 15-20% 64Cu-BaBaSar-RGD2 was trapped onto the filter and the overall recovered yield for 64Cu-BaBaSar-RGD2 is about 80% calculated from the loaded 64Cu. The radiochemical purity of 64Cu-BaBaSar-RGD2 was >99% based on radiotrace analytical HPLC (FIG. 3). The retention times for free 64CuCl2 and 64Cu-BaBaSar-RGD2 on HPLC were 2.5 and 13.9 min, respectively. The reaction crude without purifications did not show free 64Cu in HPLC chromatograms. Therefore, no further purification is needed for the final product.
    • Quality Control
  • All the quality control results met the pre-specified limits for 3 validation runs. These included half-life, appearance, pH value, identity, endotoxin amount, etc. (Table 1). The specific activity determined by HPLC analysis was between 389.2 and 605.4 mCi/μmol (average±SD, 473.0±116.2 mCi/μmol). Therefore, a human dose (<25 mCi) of 64Cu-BaBaSar-RGD2 contained less than 125 μg of RGD peptide.
  • TABLE 1
    Quality Control Data from 3 Synthesis Runs
    QC Test Release Criteria Run 1 Run 2 Run 3
    Product (mCi) none 5.5 6.2 4.5
    Visual Inspection Clear, colorless Yes Yes Yes
    Radiochemical Identity RRT = 0.9-1.1 1.0 1.0 1.0
    Radiochemical Purity >90% 99% 100% 99%
    Specific Activity >100 15.7 14.4 22.4
    (mCi/μmol)
    Dose pH 4.5-7.5 5.5 6.0 6.0
    Sterile Filter >45 64 64 62
    Integrity Test (psi)
    Radionuclidic Identity 12.6-12.8 h 12.7 12.7 12.7
    (t1/2)
    Endotoxin Analysis ≤17.5 <5 <5 <5
    (EU/mL)

    Absorbed Dose Estimates from Macaque Imaging
  • The injection of 13.1-19.7 MBq/kg of 64Cu-BaBaSar-RGD2 in macaque monkey produced no observable effects on vital signs (blood pressure, pulse, and electrocardiogram) during and 24-h after PET scan. The PET images at 1, 5, 10, 20, 40, 60, 120, and 180 min after injection are disclosed in FIG. 4. At 1 min, rapid uptake of 64Cu-BaBaSar-RGD2 was observed in the heart, and liver. The bladder content was visualized at 10 min after injection and more and more activity was accumulated in urine bladder content. The bladder did not void because the macaque monkey was under anesthesia. Gallbladder uptake was not observed during the whole scan. Rapid clearance of activity in the liver was observed in the images at time points after 1 min. The urinary bladder had the highest uptake, with 51.37%±8.73% of injected activity at 1 h post injection. The maximum uptake for the liver, and kidneys were 37.40±6.63% ID (9 min) and 26.79±4.35% ID (0.5 min) respectively. At 3 h of post injection, 8.62%±1.41% of injected activity was found in the gallbladder, small intestine, and upper and lower portions of the large intestine.
  • The mean organ doses for the male human phantom were calculated with Olinda/EXM using 64Cu-BaBaSar-RGD2 biodistribution in monkey (Table 2). The kidneys had the highest radiation-absorbed doses (108.43 μGy/MBq), followed by the bladder wall (87.07 μGy/MBq). The mean effective dose of 64Cu-BaBaSar-RGD2 was 15.30±2.21 μSv/MBq. When 925-MBq of 64Cu-BaBaSar-RGD2 is administrated into human subject, the effective dose for the non-voiding model is estimated to be 14.2 mSv, which is comparable to the estimated 6.23 mSv dose in a whole-body PET scan with 2-deoxy-2-[18F]fluoro-D-glucose (18F-FDG) (24). The estimated doses for the female human were higher by 18% because body and organ sizes of women are smaller than those men (data not shown).
  • Venous blood samples were withdrawn from monkey during the PET scan. Based on the decay corrected activity per unit of blood sample, it was found that 64Cu-BaBaSar-RGD2 was cleared rapidly from the blood. By 3 h after injection, 2.88±0.88% ID remained (range, 2.07-3.82% ID). At 22 h after injection, the activity in the blood decreased to 0.79±0.52% ID. Based on the percentage of injected dose in blood sample, the half life of 64Cu-BaBaSar-RGD2 in blood pool was calculated as 12.1±4.0 min (n=3).
  • TABLE 2
    Estimated Human Absorbed Doses of 64Cu—BaBaSar-RGD2 to
    Normal Organs Using Biodistribution Data from Macaque Monkey
    Organs Mean ± SD (μGy/MBq)
    Adrenals 3.34 ± 0.52
    Brain 1.27 ± 0.22
    Breasts 1.34 ± 0.23
    Gall bladder Wall 3.07 ± 0.49
    LLI Wall 2.86 ± 0.44
    Small Intestine 4.53 ± 0.68
    Stomach Wall 2.11 ± 0.34
    ULI Wall 2.47 ± 0.39
    Heart Wall 4.39 ± 0.62
    Kidneys 108.43 ± 16.41 
    Liver 7.54 ± 1.15
    Lungs 1.67 ± 0.28
    Muscle 1.88 ± 0.31
    Ovaries 2.88 ± 0.44
    Pancreas 2.86 ± 0.45
    Red Marrow 9.29 ± 1.02
    Osteogenic Cells 7.01 ± 0.91
    Skin 1.38 ± 0.24
    Spleen 6.78 ± 0.88
    Testes 2.03 ± 0.33
    Thymus 1.56 ± 0.26
    Thyroid 1.39 ± 0.24
    Urinary Bladder Wall 87.07 ± 12.38
    Uterus 4.16 ± 0.63
    Total Body 2.76 ± 0.42
    Effective Dose* 15.30 ± 2.21 
    *In unit of μSv/MBq
  • Integrin αvβ3-targeted radiopharmaceutical 64Cu-BaBaSar-RGD2 has been successfully synthesized with the one-step kit method. The straightforward method greatly simplifies the production process and benefits the clinical application of 64Cu-BaBaSar-RGD2. Human radiation dosimetry of 64Cu-BaBaSar-RGD2 was estimated after intravenous administration in macaque monkey, by PET imaging and OLINDA/EXM calculations. The critical organs were kidneys and urinary bladder wall. The mean effective dose, determined with the male adult model, was 15.30±2.21 μSv/MBq. This PET probe demonstrates an acceptable radiation dose comparable to other reported RGD-derived radiopharmaceuticals. These demonstrate great promise of 64Cu-BaBaSar-RGD2 as an integrin marker, with a desirable biodistribution and safety characteristics in monkey. Therefore, 64Cu-BaBaSar-RGD2 can safely be used in human scan for further evaluation of its performance as an integrin-targeting probe.
    • REFERENCES
  • All references cited herein, including those below and including but not limited to all patents, patent applications, and non-patent literature referenced below or in other portions of the specification, are hereby incorporated by reference herein in their entirety.
    • 1. Holmgren L, O'Reilly M S, Folkman J. Dormancy of micrometastases: balanced proliferation and apoptosis in the presence of angiogenesis suppression. Nat Med. 1995; 1:149-153.
    • 2. Parangi S, O'Reilly M, Christofori G, Holmgren L, Grosfeld J, Folkman J, Hanahan D. Antiangiogenic therapy of transgenic mice impairs de novo tumor growth. Proc Natl Acad Sci USA. 1996; 93:2002-2007.
    • 3. Brown A P, Citrin D E, Camphausen K A. Clinical biomarkers of angiogenesis inhibition. Cancer Metastasis Rev. 2008; 27:415-434.
    • 4. Brooks P C, Montgomery A M, Rosenfeld M, Reisfeld R A, Hu T, Klier G, Cheresh D A. Integrin alpha v beta 3 antagonists promote tumor regression by inducing apoptosis of angiogenic blood vessels. Cell. 1994; 79:1157-1164.
    • 5. Marelli U K, Rechenmacher F, Sobahi T R, Mas-Moruno C, Kessler H. Tumor Targeting via Integrin Ligands. Front Oncol. 2013; 3:222.
    • 6. Plow E F, Haas T A, Zhang L, Loftus J, Smith J W. Ligand binding to integrins. J Biol Chem. 2000; 275:21785-21788.
    • 7. Liu S. Radiolabeled Cyclic RGD Peptide Bioconjugates as Radiotracers Targeting Multiple Integrins. Bioconjug Chem. 2015; 26:1413-1438.
    • 8. Beer A J, Haubner R, Wolf I, Goebel M, Luderschmidt S, Niemeyer M, Grosu A L, Martinez M J, Wester H J, Weber W A, Schwaiger M. PET-based human dosimetry of 18F-galacto-RGD, a new radiotracer for imaging alpha v beta3 expression. J Nucl Med. 2006; 47:763-769.
    • 9. Haubner R, Kuhnast B, Mang C, Weber W A, Kessler H, Wester H J, Schwaiger M. [18F]Galacto-RGD: synthesis, radiolabeling, metabolic stability, and radiation dose estimates. Bioconjug Chem. 2004; 15:61-69.
    • 10. Morrison M S, Ricketts S A, Barnett J, Cuthbertson A, Tessier J, Wedge S R. Use of a novel Arg-Gly-Asp radioligand, 18F-AH111585, to determine changes in tumor vascularity after antitumor therapy. J Nucl Med. 2009; 50:116-122.
    • 11. McParland B J, Miller M P, Spinks T J, Kenny L M, Osman S, Khela M K, Aboagye E, Coombes R C, Hui A M, Cohen P S. The biodistribution and radiation dosimetry of the Arg-Gly-Asp peptide 18F-AH111585 in healthy volunteers. J Nucl Med. 2008; 49:1664-1667.
    • 12. Kenny L M, Coombes R C, Oulie I, Contractor K B, Miller M, Spinks T J, McParland B, Cohen P S, Hui A M, Palmieri C, Osman S, Glaser M, Turton D, Al-Nahhas A, Aboagye E O. Phase I trial of the positron-emitting Arg-Gly-Asp (RGD) peptide radioligand 18F-AH111585 in breast cancer patients. J Nucl Med. 2008; 49:879-886.
    • 13. Zhou Y, Gao S, Huang Y, Zheng J, Dong Y, Zhang B, Zhao S, Lu H, Liu Z, Yu J, Yuan S. A Pilot Study of 18F-Alfatide PET/CT Imaging for Detecting Lymph Node Metastases in Patients with Non-Small Cell Lung Cancer. Sci Rep. 2017; 7:2877.
    • 14. Wan W, Guo N, Pan D, Yu C, Weng Y, Luo S, Ding H, Xu Y, Wang L, Lang L, Xie Q, Yang M, Chen X. First experience of 18F-alfatide in lung cancer patients using a new lyophilized kit for rapid radiofluorination. J Nucl Med. 2013; 54:691-698.
    • 15. Mirfeizi L, Walsh J, Kolb H, Campbell-Verduyn L, Dierckx R A, Feringa B L, Elsinga P H, de Groot T, Sannen I, Bormans G, Celen S. Synthesis of [18F]RGD-K5 by catalyzed [3+2] cycloaddition for imaging integrin alphavbeta3 expression in vivo. Nucl Med Biol. 2013; 40:710-716.
    • 16. Doss M, Kolb H C, Zhang J J, Belanger M J, Stubbs J B, Stabin M G, Hostetler E D, Alpaugh R K, von Mehren M, Walsh J C, Haka M, Mocharla V P, Yu J Q. Biodistribution and radiation dosimetry of the integrin marker 18F-RGD-K5 determined from whole-body PET/CT in monkeys and humans. J Nucl Med. 2012; 53:787-795.
    • 17. lagaru A, Mosci C, Mittra E, Zaharchuk G, Fischbein N, Harsh G, Li G, Nagpal S, Recht L, Gambhir S S. Glioblastoma Multiforme Recurrence: An Exploratory Study of (18)F FPPRGD2 PET/CT. Radiology. 2015; 277:497-506.
    • 18. Iagaru A, Mosci C, Shen B, Chin F T, Mittra E, Telli M L, Gambhir S S. (18)F-FPPRGD2 PET/CT: pilot phase evaluation of breast cancer patients. Radiology. 2014; 273:549-559.
    • 19. Mena E, Owenius R, Turkbey B, Sherry R, Bratslaysky G, Macholl S, Miller M P, Somer E J, Lindenberg L, Adler S, Shih J, Choyke P, Kurdziel K. [(1)(8)F]fluciclatide in the in vivo evaluation of human melanoma and renal tumors expressing alphavbeta 3 and alpha vbeta 5 integrins. Eur J Nucl Med Mol Imaging. 2014; 41:1879-1888.
    • 20. Li D, Zhao X, Zhang L, Li F, Ji N, Gao Z, Wang J, Kang P, Liu Z, Shi J, Chen X, Zhu Z. (68)Ga-PRGD2 PET/CT in the evaluation of Glioma: a prospective study. Mol Pharm. 2014; 11:3923-3929.
    • 21. Chatziioannou A, Tai Y C, Doshi N, Cherry S R. Detector development for microPET II: a 1 microl resolution PET scanner for small animal imaging. Phys Med Biol. 2001; 46:2899-2910.
    • 22. Smith S V. Molecular imaging with copper-64. J Inorg Biochem. 2004; 98:1874-1901.
    • 23. Liu S, Li Z, Yap L P, Huang C W, Park R, Conti P S. Efficient preparation and biological evaluation of a novel multivalency bifunctional chelator for 64Cu radiopharmaceuticals. Chemistry. 2011; 17:10222-10225.
    • 24. Huang B, Law M W, Khong P L. Whole-body PET/CT scanning: estimation of radiation dose and cancer risk. Radiology. 2009; 251:166-174.
    • 25. U.S. Pat. No. 9,789,211.

Claims (6)

What is claimed is:
1. A kit for comprising:
a lyophilized powder of BaBaSar-RGD2, a buffer salt and
instructions for reconstituting the lyophilized powder with a 64Cu solution comprising 64CuCl2.
2. The kit according to claim 1, wherein the buffer salt is sodium acetate buffer.
3. The kit according to claim 1, wherein the instructions comprise adding the 64Cu solution to the lyophilized powder.
4. The kit according to claim 1, comprising about 50 μg BaBaSar-RGD2.
5. The kit according to claim 1, wherein the kit has a shelf life of over three months at room temperature.
6. The kit according to claim 1, wherein the kit has a shelf life of over a year when stored at a temperature of 2-8° C.
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080267882A1 (en) * 2007-04-27 2008-10-30 Stanford University Imaging compounds, methods of making imaging compounds, methods of imaging, therapeutic compounds, methods of making therapeutic compounds, and methods of therapy
US9403875B2 (en) * 2009-01-27 2016-08-02 University Of Southern California Cage-like bifunctional chelators, copper-64 radiopharmaceuticals and PET imaging using the same
US9789211B2 (en) * 2012-05-14 2017-10-17 University Of Southern California Methods and compositions for positron emission tomography myocardial perfusion imaging
US11253617B2 (en) * 2017-12-01 2022-02-22 University Of Southern California One step 64Cu-BaBaSar-RGD2 production method

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Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080267882A1 (en) * 2007-04-27 2008-10-30 Stanford University Imaging compounds, methods of making imaging compounds, methods of imaging, therapeutic compounds, methods of making therapeutic compounds, and methods of therapy
US9403875B2 (en) * 2009-01-27 2016-08-02 University Of Southern California Cage-like bifunctional chelators, copper-64 radiopharmaceuticals and PET imaging using the same
US9789211B2 (en) * 2012-05-14 2017-10-17 University Of Southern California Methods and compositions for positron emission tomography myocardial perfusion imaging
US11253617B2 (en) * 2017-12-01 2022-02-22 University Of Southern California One step 64Cu-BaBaSar-RGD2 production method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Liu et al, Chem. Eur. J., Vol. 17, pages 10222-10225 with Supporting Information (7 pages) (Year: 2011) *

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