[go: up one dir, main page]

US20210002673A1 - Microorganisms for producing 4c-5c compounds with unsaturation and methods related thereto - Google Patents

Microorganisms for producing 4c-5c compounds with unsaturation and methods related thereto Download PDF

Info

Publication number
US20210002673A1
US20210002673A1 US16/888,339 US202016888339A US2021002673A1 US 20210002673 A1 US20210002673 A1 US 20210002673A1 US 202016888339 A US202016888339 A US 202016888339A US 2021002673 A1 US2021002673 A1 US 2021002673A1
Authority
US
United States
Prior art keywords
coa
enzyme
buten
hydroxypent
butadiene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US16/888,339
Inventor
Stephanie J. Culler
Mark J. Burk
Robin E. Osterhout
Priti Pharkya
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genomatica Inc
Original Assignee
Genomatica Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genomatica Inc filed Critical Genomatica Inc
Priority to US16/888,339 priority Critical patent/US20210002673A1/en
Publication of US20210002673A1 publication Critical patent/US20210002673A1/en
Abandoned legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

Definitions

  • the present invention relates generally to metabolic and biosynthetic processes and microbial organisms capable of producing organic compounds, and more specifically to non-naturally occurring microbial organisms having an organic compound pathway, such as butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and 2,4-pentadienoate.
  • organic compound pathway such as butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and 2,4-pentadienoate.
  • butadiene 1,3-butadiene, “BD”) are produced annually and is applied in the manufacture of polymers such as synthetic rubbers and ABS resins, and chemicals such as hexamethylenediamine and 1,4-butanediol.
  • BD butadiene
  • alkenes e.g. styrene
  • SBR styrene-1,3-butadiene
  • Butadiene is typically produced as a by-product of the steam cracking process for conversion of petroleum feedstocks such as naphtha, liquefied petroleum gas, ethane or natural gas to ethylene and other olefins.
  • butadiene renewably involves fermentation of sugars or other feedstocks to produce diols, such as 1,4-butanediol or 1,3-butanediol, which are separated, purified, and then dehydrated to butadiene in a second step involving metal-based catalysis.
  • diols such as 1,4-butanediol or 1,3-butanediol
  • Crotyl alcohol also referred to as 2-buten-1-ol
  • Crotyl alcohol also referred to as 2-buten-1-ol
  • It serves as a precursor to crotyl halides, esters, and ethers, which in turn are chemical intermediates in the production of monomers, fine chemicals, agricultural chemicals, and pharmaceuticals.
  • Exemplary fine chemical products include sorbic acid, trimethylhydroquinone, crotonic acid and 3-methoxybutanol.
  • CrotOH is also a precursor to 1,3-butadiene. CrotOH is currently produced exclusively from petroleum feedstocks. For example Japanese Patent 47-013009 and U.S. Pat. Nos.
  • 3,090,815, 3,090,816, and 3,542,883 describe a method of producing CrotOH by isomerization of 1,2-epoxybutane.
  • the ability to manufacture CrotOH from alternative and/or renewable feedstocks would represent a major advance in the quest for more sustainable chemical production processes.
  • 3-Buten-2-ol (also referenced to as methyl vinyl carbinol (“MVC”)) is an intermediate that can be used to produce butadiene.
  • MVC methyl vinyl carbinol
  • MVC can also be used as a solvent, a monomer for polymer production, or a precursor to fine chemicals. Accordingly, the ability to manufacture MVC from alternative and/or renewable feedstock would again present a significant advantage for sustainable chemical production processes.
  • 2,4-Pentadienoate is a useful substituted butadiene derivative in its own right and a valuable intermediate en route to other substituted 1,3-butadiene derivatives, including, for example, 1-carbamoyl-1,3-butadienes which are accessible via Curtius rearrangement.
  • the resultant N-protected-1,3-butadiene derivatives can be used in Diels alder reactions for the preparation of substituted anilines.
  • 2,4-Pentadienoate can be used in the preparation of various polymers and co-polymers.
  • the invention provides a non-naturally occurring microbial organism having a pathway to butadiene, crotyl alcohol, 2,4-pentadienoate, or 3-buten-2-ol, said microbial organism comprising at least one exogenous nucleic acid encoding a pathway enzyme, wherein the enzyme is selected from the group consisting of (1E) 4-hydroxy 2-oxovalerate decarboxylase, (1 D) 3-hydroxybutyraldehyde dehydratase, (1N) 2-oxopent-3-enoyl-CoA synthetase or transferase, (1O) 2-oxopent-3-enoyl-CoA reductase, (1P) 2-hydroxypent-3-enoyl-CoA dehydratase/vinylisomerase, (1R) 2-hydroxypent-3-enoyl-CoA synthetase or transferase, (1F) 2-oxopent-3-enoate reductase, (1E)
  • One or more additional pathway enzymes may be included in the microbial organism.
  • the invention also provides methods for the production of butadiene, crotyl alcohol, 2,4-pentadienoate, or 3-buten-2-ol, comprising culturing the non-naturally occurring microorganism.
  • the invention provides a non-naturally occurring microbial organism having a pathway to butadiene, crotyl alcohol, 2,4-pentadienoate, or 3-buten-1-ol, said microbial organism comprising at least one exogenous nucleic acid encoding a pathway enzyme, the enzyme selected from the group consisting of (2E) 2-hydroxypent-4-enoate vinylisomerase, (2H) 2-hydroxypent-4-enoate mutase, and (2M) 3-hydroxypent-4-enoyl-CoA vinylisomerase.
  • One or more additional pathway enzymes may be included in the microbial organism.
  • the invention also provides methods for the production of butadiene, crotyl alcohol, 2,4-pentadienoate, or 3-buten-1-ol, comprising culturing the non-naturally occurring microorganism.
  • the invention provides a non-naturally occurring microbial organism having a pathway to butadiene, 2,4-pentadienoate, or 3-buten-2-ol, said microbial organism comprising at least one exogenous nucleic acid encoding a pathway enzyme, the enzyme selected from the group consisting of (3D) 3,4-dihydroxypentanoate dehydratase, (3E) 4-hydroxypent-2-enoate decarboxylase (3H) 3,4-dihydroxypentanoyl-CoA dehydratase, and (3J) 4-hydroxypent-2-enoyl-CoA transferase.
  • the invention also provides methods for the production of butadiene, crotyl alcohol, 2,4-pentadienoate, or 3-buten-2-ol, comprising culturing the non-naturally occurring microorganism.
  • the invention provides a non-naturally occurring microbial organism having a pathway to convert crotyl alcohol to butadiene or 3-buten-2-ol, said microbial organism comprising at least one exogenous nucleic acid encoding a pathway enzyme, wherein the enzyme is selected from the group consisting of (4D) 3-buten-2-ol synthase, (4E) 3-buten-2-ol synthase, or (4F) crotyl alcohol isomerase.
  • FIG. 1 shows pathways to butadiene from pyruvate and acetyl-CoA.
  • Enzymes are A. acetyl-CoA reductase, B. 4-hydroxy 2-oxovalerate aldolase, C. 4-hydroxy 2-oxovalerate decarboxylase, D. 3-hydroxybutyraldehyde dehydratase, E. 4-hydroxy-2-oxovalerate 3-dehydratase, F. 2-oxopent-3-enoate reductase, G. 2-hydroxypent-3-enoate dehydratase/vinylisomerase, H. 2,4-pentadienoate decarboxylase, I. 2-oxopent-3-enoate decarboxylase, J.
  • crotyl aldehyde reductase K. crotyl alcohol vinylisomerase, L. 3-buten-2-ol dehydratase, M. crotyl alcohol dehydratase/vinylisomerase, N. 2-oxopent-3-enoyl-CoA synthetase or transferase, O. 2-oxopent-3-enoyl-CoA reductase, P. 2-hydroxypent-3-enoyl-CoA dehydratase/vinylisomerase, Q. 2,4-pentadienoyl-CoA synthetase, transferase or hydrolase, R.
  • 2-hydroxypent-3-enoyl-CoA synthetase or transferase S. 2-hydroxypent-3-enoate decarboxylase, T. 4-hydroxypent-2-enoate decarboxylase, U. 2-hydroxypent-3-enoate vinylisomerase, V. 4-hydroxypent-2-enoate dehydratase, W. vinylisomerase.
  • FIG. 2 shows pathways to butadiene from pyruvate and acetyl-CoA.
  • Acetyl-CoA reductase B. 4-hydroxy 2-oxovalerate aldolase, C. 4-hydroxy 2-oxovalerate dehydratase, D. 2-oxopent-4-enoate reductase, E. 2-hydroxypent-4-enoate vinylisomerase, F. 5-hydroxypent-2-enoate dehydratase, G. 2,4-pentadienoate decarboxylase, H. 2-hydroxypent-4-enoate mutase, I. 5-hydroxypent-2-enoyl-CoA synthetase, transferase or hydrolase, J.
  • FIG. 3 shows pathways to but-3-enol and/or butadiene from lactoyl-CoA and acetyl-CoA.
  • Enzymes are A. 3-Oxo-4-hydroxypentanoyl-CoA thiolase, B. 3-oxo-4-hydroxypentanoyl-CoA transferase, synthetase or hydrolase, C. 3-oxo-4-hydroxypentanoate reductase, D. 3,4-dihydroxypentanoate dehydratase, E. 4-hydroxypent-2-enoate decarboxylase, F. 3-buten-2-ol dehydratase, G.
  • 3-oxo-4-hydroxypentanoyl-CoA reductase H. 3,4-dihydroxypentanoyl-CoA dehydratase, I. 3,4-dihydroxypentanoyl-CoA transferase, synthetase or hydrolase, J. 4-hydroxypent-2-enoyl-CoA transferase, synthetase or hydrolase, K. 4-hydroxypent-2-enoate dehydratase, L. 2,4-pentadienoate decarboxylase, M. 4-hydroxypent-2-enoyl-CoA dehydratase, N. 2,4-pentadienoyl-CoA hydrolase, transferase or synthetase, O. vinylisomerase.
  • FIG. 4 shows pathways for converting crotyl alcohol (2-Buten-1-ol) to 3-buten-2-ol and/or butadiene.
  • Enzymes are A. crotyl alcohol kinase, B. 2-butenyl-4-phosphate kinase, C. butadiene synthase, D. 3-buten-2-ol synthase, E. 3-buten-2-ol synthase, F. crotyl alcohol isomerase, G. 3-buten-2-ol dehydratase, H. crotyl alcohol dehydratase, I. crotyl alcohol diphosphokinase, J. butadiene synthase (from 2-butenyl-4-phosphate).
  • FIG. 5 shows the GCMS analysis of an authentic sample of MVC or 3-buten-2-ol.
  • FIG. 6 shows the GCMS chromatograms obtained for the enzymatic (blue) assay with 50 mM CrotOH after 16 hours and 1 mM standard of MVC or 3-buten-2-ol dissolved in minimal media.
  • FIG. 7 is a graph showing activity measurements for assessing the NADPH-dependent reduction of crotonaldehyde to crotonol.
  • FIG. 8 is a graph of in vitro formation of crotyl phosphate (CrPi) and ADP over time from a composition including ATP, crotyl alcohol, and hydroxyethylthiazole kinase.
  • product pathways described herein are specifically contemplated for use in the organisms, compositions and their uses, such as in methods to make the target products of 1,3-butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol or 2,4-pentadienoate or other product, and other embodiments as taught herein.
  • Embodiments of the disclosure are directed to the design of metabolic pathways, and the production and use of non-naturally occurring microbial organisms capable of producing butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol and/or 2,4-pentadienoate.
  • metabolic pathways can be designed and recombinantly engineered to achieve the biosynthesis of butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate in microbial organisms such as Escherichia coli and other cells or organisms.
  • Biosynthetic production of butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate can be performed by construction and fermentation of strains having the designed metabolic genotype.
  • FIG. 1 provides exemplary pathways to butadiene from acetyl-CoA and pyruvate via intermediate 2-oxopent-3-enoate (step E) or 3-hydroxybutyraldehyde (step C). Also shown are pathways to the intermediate products (which may be desirably obtained as final products) of crotol alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate, which in certain pathway routes, can be intermediates in butadiene synthesis.
  • acetyl-CoA is converted to acetaldehyde by acetyl-CoA reductase, and pyruvate and acetaldehyde are then converted to 4-hydroxy-2-oxovalerate by 4-hydroxy 2-oxovalerate aldolase.
  • the intermediate 4-hydroxy-2-oxovalerate can be promoted to enter different pathways depending on the metabolic design.
  • 4-hydroxy-2-oxovalerate can be converted to 2-oxopent-3-enoate in the presence of 4-hydroxy-2-oxovalerate 3-dehydratase.
  • Pathway “E” can branch off into one or more different pathways depending on the metabolic design. For example, in pathway “E ⁇ N” 2-oxopent-3-enoate is converted to 2-oxopent-3-enoyl-CoA in the presence of 2-oxopent-3-enoyl-CoA synthetase or transferase (N); subsequent enzymatic conversions of intermediates can occur.
  • pathway “E ⁇ F” 2-oxopent-3-enoate is converted to 2-hydroxypent-3-enoate in the presence of 2-oxopent-3-enoate reductase (F); subsequent enzymatic conversions of intermediates can occur.
  • the intermediate 2,4-pentadienoate can be obtained further downstream in the E ⁇ F branch of the pathway.
  • pathway “E ⁇ I” 2-oxopent-3-enoate is converted to crotyl aldehyde in the presence of 2-oxopent-3-enoate decarboxylase (I); subsequent enzymatic conversions of intermediates can occur.
  • the intermediates such as crotol alcohol and/or 3-buten-2-ol can be obtained further downstream in the E ⁇ I branch of the pathway.
  • the intermediate 4-hydroxy-2-oxovalerate can also be promoted to enter pathway (“C”), where 4-hydroxy-2-oxovalerate can be converted to 3-hydroxy butyraldehyde in the presence of 4-hydroxy 2-oxovalerate decarboxylase.
  • 4-hydroxy 2-oxovalerate decarboxylase can be converted to crotyl aldehyde in the presence of 3-hydroxybutyraldehyde dehydratase (D).
  • Crotyl aldehyde can be converted to crotyl alcohol, which can be an intermediate in the pathway, or can be obtained as a final product, by crotyl aldehyde reductase (J).
  • crotyl alcohol can be converted to 3-buten-2-ol, which can be an intermediate in the pathway, or can be obtained as a final product, by crotyl alcohol vinylisomerase (K).
  • 3-buten-2-ol from steps K or T can be isolated or from step T converted to crotyl alcohol enzymatically (W) or chemically.
  • Crotyl alcohol can enter FIG. 4 or be isolated.
  • the non-naturally occurring microbial organism can have any one of the following pathways.
  • the pathway enzymes is encoded by nucleic acid that is heterologous to the host species.
  • the heterologous nucleic acid can be obtained from a microbial species other than the host species, such as a nucleic acid from a bacteria other than E. coli (i.e., a non- E. coli bacteria), wherein the heterologous nucleic acid is transformed into E. coli host organism to create any of the pathways of the disclosure.
  • two, three, four, five, six, etc., heterologous nucleic acids from one or more bacteria other than the host bacteria are transformed into the bacterial host organism to create any of the pathways of the disclosure.
  • a pathway such as BEFUVH in a host organism one can first determine what, if any, enzymes of the BEFUVH are naturally present in a desired host species. If B is native to the host species, but not EFUVH, the host can then be transformed with heterologous nucleic acids encoding EFUVH to create a pathway in the cell to form butadiene.
  • This approach can be used to create a non-naturally occurring microbial organism having a pathway to butadiene, crotyl alcohol, 2,4-pentadienoate, or 3-buten-2-ol, having any of the pathways of the disclosure.
  • FIG. 2 provides exemplary pathways to butadiene from acetyl-CoA and pyruvate via intermediate 5-hydroxypent-2-enoate (step E) or 3-hydroxypent-4-enoate (step H). Also shown are pathways to the intermediate products (which may be desirably obtained as final products) of crotol alcohol, 3-buten-1-ol and/or 2,4-pentadienoate, which in certain pathway routes, can be intermediates in butadiene synthesis.
  • acetyl-CoA is converted to acetaldehyde by acetyl-CoA reductase (A), and pyruvate and acetaldehyde then converted to 4-hydroxy-2-oxovalerate by 4-hydroxy 2-oxovalerate aldolase (B).
  • 4-hydroxy-2-oxovalerate is converted to 2-oxopent-4-enoate by 4-hydroxy 2-oxovalerate dehydratase (C), and then 2-oxopent-4-enoate is converted to 2-hydroxypent-4-enoate by 2-oxopent-4-enoate reductase (D).
  • the intermediate 2-hydroxypent-4-enoate can enter pathway branch “E” or “H.”
  • pathway branch E 2-hydroxypent-4-enoate is converted to 5-hydroxypent-2-enoate in the presence of 2-hydroxypent-4-enoate vinylisomerase.
  • the intermediate 5-hydroxypent-2-enoate can then enter one or more of pathway branches (P, F, and/or I) before conversion to butadiene.
  • 3-buten-1-ol can be converted to crotyl alcohol (S), which can be an intermediate in the pathway (entering FIG. 4 ), or can be obtained as a final product.
  • S crotyl alcohol
  • pathway branch H 2-hydroxypent-4-enoate is converted to 3-hydroxypent-4-enoate in the presence of 2-hydroxypent-4-enoate mutase.
  • the intermediate 3-hydroxypent-4-enoate can then enter one or more of pathway branches (R, K, and/or L) before conversion to butadiene.
  • the non-naturally occurring microbial organism can have any one of the following pathways. E; DE; CDE; BCDE; ABCDE; EF; EFG; EP; EPQ; EI; EIN; EINJ; EINJG; ABCDEF; ABCDEFG; ABCDEP; ABCDEPQ; ABCDEI; ABCDEIN; ABCDEINJ; ABCDEINJG; BCDEF; BCDEFG; BCDEP; BCDEPQ; BCDEI; BCDEIN; BCDEINJ; BCDEINJG; CDEF; CDEFG; CDEP; CDEPQ; CDEI; CDEIN; CDEINJ; CDEINJG; DEF; DEFG; DEP; DEPQ; DEI; DEIN; DEINJ; DEINJG; H; DH; CDH; BCDH; ABCDH; HL; HLM; HLMN; HLMNJ; HLMNJG; HLO; HLOJ; HLOJG; HLMI; HLMIF;
  • Step S conversion to crotyl alcohol.
  • BCDEPS BCDEPS; CDEPS; DEPS; HLMIPS; ABCDHLMIPS; BCDHLMIPS; CDHLMIPS and DHLMIPS.
  • FIG. 3 provides exemplary pathways to butadiene from lactoyl-CoA and acetyl-CoA via intermediate 4-hydroxypent-2-enoate, 3-buten-2-ol, 4-hydroxypent-2-enoyl-CoA, 4-hydroxypent-2-enoate; enzymes such as 3,4-dihydroxypentanoate dehydratase (step D), 4-hydroxypent-2-enoate decarboxylase (step E), 3,4-dihydroxypentanoyl-CoA dehydratase (step H), and 4-hydroxypent-2-enoyl-CoA transferase (step J) can be used.
  • 3-buten-2-ol from step E can be isolated as final product or converted to crotyl alcohol enzymatically (O) or chemically. Crotyl alcohol can be isolated or enter FIG. 4 .
  • the non-naturally occurring microbial organism can have any one of the following pathways: E; EF; D; DE; DEF; DK; DKL; J; JE; JEF; JK; JKL; H; HJ; HJE; HJEF; HJK; HJKL; HM; HMN; HMNL; GH; GHJ; GHJE; GHJEF; GHJK; GHJKL; GHM; GHMN; GHMIL; AGH; AGHJ; AGHJE; AGHJEF; AGHJK AGHJKL; AGHM; AGHMN; AGHMNL; ID; IDE; IDEF; IDK; IDKL; GID; GIDE; GIDEF; GIDK; GIDKL; AGID; AGIDE; AGIDEF; AGIDK; AGIDKL; CD; CDE; CDEF; CDK; CDKL; BCD; BCDE; BCDEF; BCDK; BCDKL; CD; CDE; CD
  • Step O conversion to crotyl alcohol.
  • EO DEO
  • JEO JEO
  • HJEO AGHJEO
  • IDEO GIDEO
  • AGIDEO CDEO
  • BCDEO BCDEO
  • ABCDEO ABCDEO
  • GHJEO GHJEO
  • FIG. 4 provides exemplary pathways to butadiene from crotyl alcohol via one or more of the following intermediates: 2-butenyl-4-phosphate, 2-butenyl-4-diphosphate, and/or 3-buten-2-ol.
  • crotyl alcohol can be introduced into this pathway via any crotyl alcohol-producing pathway known in the art, or as described herein (e.g., from precursors crotyl aldehyde as converted to crotyl alcohol in the presence of crotyl aldehyde reductase (1J); or 2-hydroxypent-3-enoate converted to crotyl alcohol in the presence of 2-hydroxypent-3-enoate decarboxylase (1S) and including conversion from 3-buten-1-ol or 3-buten-2-ol).
  • the crotyl alcohol is produced by a pathway of FIG. 1, 2 or 3 and then introduced into a pathway of FIG. 4 .
  • step A of FIG. 4 uses a hydroxyethylthiazole kinase, a thiamine kinase, a pantothenate kinase, a 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, a riboflavin kinase, a L-fuculokinase, and/or a choline kinase, such as those described herein.
  • step B of FIG. 4 subset of kinases of the EC 2.7.1.a class suitable for FIG. 4 Step B include 2.7.4.a Phosphokinases listed below.
  • 2-Butenyl-4-phosphate kinase enzymes catalyze the transfer of a phosphate group to the phosphate group of 2-butenyl-4-phosphate ( FIG. 4B ).
  • the non-naturally occurring microbial organism can have any one of the following pathways: D; DG; AD; ADG; E; EG; BE; BEG; ABE; ABEG; IE; IEG; F; FG; G; H; J; AJ; A; AB; ABC and each combination of any one or more of steps D, E, F with any one or more of steps of FIG. 4 , wherein when at least one of 4A, 4B, 4C, 4G or 4H is present then (i) at least one other unique step or pathway is present, such as step 4D, 4E, any unique step or pathway from FIG. 1 (e.g.
  • Step 4A, 4B, 4C, 4G or 4H or (ii) at least one of A, B, C, G or H consists of a specific sub-group of enzyme classes or enzymes described herein as A for Step A, as B for Step B, as C for Step C, as G for Step G or as H for Step H.
  • each pathway or step above is also contemplated the substitution of any one or more of subsets of enzymes described as Steps 1L, 1V, 1M, 2C, 2F, 2O, 2N, 2Q, 2R, 3F, 3K, 3M, 4A, 4B, 4C, 4G and 4H for its corresponding step (e.g. replace enzymes described for Step 1L with those described for Step 1L*).
  • crotyl alcohol or 3-buten-2-ol is produced by a pathway of FIG. 1 (e.g., a pathway such as: ABCDJ; ABEIJ; ABEFS; BCDJ; BEIJ: BEFS; CDJ; EIJ; EFS; and DJ, optionally further with step W), or is produced by a pathway of FIG. 3 , and then introduced into a pathway of FIG. 4 by combining with a pathway of FIG. 4 selected from: A; AB; ABC; AJ; AD; ADG; ABE; ABEG; F; FG; I; IC; IE; IEG; H; and G.
  • a pathway of FIG. 1 e.g., a pathway such as: ABCDJ; ABEIJ; ABEFS; BCDJ; BEIJ: BEFS; CDJ; EIJ; EFS; and DJ, optionally further with step W
  • a pathway of FIG. 3 e.g., a pathway such as: ABCDJ; ABEIJ
  • pathway having substitution of any one or more of subsets of enzymes described in Steps 4G and 4H for its corresponding step e.g. FIG. 1 ABCDJ plus FIG. 4 IEG becomes FIG. 1 ABCDJ plus FIG. 4 IEG*).
  • crotyl alcohol or 3-buten-1-ol is produced by a pathway of FIG. 2 and then introduced into a pathway of FIG. 4 by combining with a pathway of FIG. 4 selected from: A; AB; ABC; AJ; AD; ADG; ABE; ABEG; F; FG; I; IC; IE; IEG; H; and G.
  • pathway having substitution of any one or more of subsets of enzymes described in Steps 4G and 4H for its corresponding step e.g. FIG. 2 ABCDEPS plus FIG. 4 IEG becomes FIG. 2 ABCDEPS plus FIG. 4 IEG*).
  • Steps 1N, 1O, 1P, 1R, 1F, U and 1K are also specifically contemplated herein as unique transformations of FIG. 2 in addition to Step 2H and 2E.
  • Step 2M Also specifically contemplated herein as unique transformations of FIG. 4 in addition to Step 4D and 4E is Step 4F.
  • Steps 1L, 1V, 1M, 2C, 2F, 2O, 2N, 2Q, 2R, 3F, 3K, 3M, 4A, 4B, 4C, 4G and 4H are unique. Accordingly, all subject matter specifically expressed herein applies equally to these additional unique steps.
  • a non-naturally occurring microbial organism having a pathway to butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-1-ol, 3-buten-2-ol, or other product said microbial organism comprising a nucleic acid encoding a pathway enzyme for the unique step, as well as methods of use described herein.
  • a step A subset of kinases of FIG. 4 Step A include enzymes exemplified in the tables below for hydroxyethylthiazole kinase, thiamine kinase, pantothenate kinase, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, riboflavin kinase, L-fuculokinase and choline kinase.
  • the table below provides hydroxyethyl thiazole kinases, including EC 2.7.1.50 class, for FIG. 4 , Step A:
  • Exemplary candidate thiamine kinases for FIG. 4 , Step A are:
  • Exemplary fuculokinases for FIG. 4 , Step A are:
  • Step A Exemplary 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase for FIG. 4 , Step A are:
  • Exemplary pantothenate kinases for FIG. 4 , Step A are:
  • Step A Exemplary 4-(cytidine 5′-diphospho)-2-C-methyl-D-erythritol kinases (2.7.1.148) for FIG. 4 , Step A are:
  • Step A subset of synthases (alkene forming) for FIG. 4 Step C include 4-dimethylallyltryptophan synthase (class EC 2.5.1.34) and dimethylallyltranstransferase (class EC 2.5.1.1) that catalyze the conversion of 2-butenyl-4-diphosphate to 1,3-butadiene (Butadiene Synthase (BDS).
  • BDS butadiene Synthase
  • the enzymes in these classes naturally possess such activity or can be engineered to exhibit or enhance this activity.
  • the enzyme-subsets designated as steps 1L, 1V, 1M, 2C, 2F, 2O, 2N, 2Q, 2R, 3F, 3K, 3M, 4G and 4H of dehydratase of the EC 4.2.1.a class for their respective Steps 1L, 1V, 1M, 2C, 2F, 2O, 2N, 2Q, 2R, 3F, 3K, 3M, 4G and 4H include the exemplary dehydratases of the 4.2.1.a class shown in the table below as well as a dehydratase class that dehydrates phenyllactyl-CoA to cinnamoyl-CoA exemplified by the dehydratase found in Clostridium sporogens that dehydrates phenyllactyl-CoA to cinnamoyl-CoA.
  • This enzyme is composed of three subunits, one of which is a CoA transferase.
  • the first step comprises of a CoA transfer from cinnamoyl-CoA to phenyllactate leading to the formation of phenyllactyl-CoA and cinnamate.
  • the product cinnamate is released.
  • the dehydratase then converts phenyllactyl-CoA into cinnamoyl-CoA.
  • the FldA is the CoA transferase and FldBC are alpha and beta subunits of the dehydratase, which are related to component D from A. fermentans .
  • FIG. 4G and FIG. 4H subsets and FIG. 1 Step L, V and M subsets and FIG. 2 Step C, F, N, O, Q and R subsets, and FIG. 3 Steps F, K and M subsets are subsets of dehydratases for their respective Steps 1L, 1V, 1M, 2C, 2F, 2O, 2N, 2Q, 2R, 3F, 3K, 3M, 4G and 4H are listed in the following table.
  • FIG. 4G and FIG. 4H subsets and FIG. 1 Step L, V and M subsets and FIG. 2 Step C, F, N, O, Q and R subsets, and FIG. 3 Steps F, K and M subsets include dimethylmaleate hydratases that catalyze the dehydration of (2R,3S)-2,3-dimethylmalate into dimethylmaleate (EC 4.2.1.85) shown in the table below.
  • Enzymes with dehydratase and vinylisomerase activity suitable for FIG. 1 Steps G and M subsets and FIG. 4 Steps G and H subsets include bifunctional enzymes with dehydratase and isomerase activities exemplified below.
  • non-naturally occurring when used in reference to a microbial organism or microorganism of the invention is intended to mean that the microbial organism has at least one genetic alteration not normally found in a naturally occurring strain of the referenced species, including wild-type strains of the referenced species.
  • Genetic alterations include, for example, modifications introducing expressible nucleic acids encoding metabolic polypeptides, other nucleic acid additions, nucleic acid deletions and/or other functional disruption of the microbial organism's genetic material. Such modifications include, for example, coding regions and functional fragments thereof, for heterologous, homologous or both heterologous and homologous polypeptides for the referenced species.
  • Additional modifications include, for example, non-coding regulatory regions in which the modifications alter expression of a gene or operon.
  • Exemplary metabolic polypeptides include enzymes or proteins within a butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate biosynthetic pathway.
  • a metabolic modification refers to a biochemical reaction that is altered from its naturally occurring state. Therefore, non-naturally occurring microorganisms can have genetic modifications to nucleic acids encoding metabolic polypeptides, or functional fragments thereof. Exemplary metabolic modifications are disclosed herein.
  • the term “isolated” when used in reference to a microbial organism is intended to mean an organism that is substantially free of at least one component as the referenced microbial organism is found in nature.
  • the term includes a microbial organism that is removed from some or all components as it is found in its natural environment.
  • the term also includes a microbial organism that is removed from some or all components as the microbial organism is found in non-naturally occurring environments. Therefore, an isolated microbial organism is partly or completely separated from other substances as it is found in nature or as it is grown, stored or subsisted in non-naturally occurring environments.
  • Specific examples of isolated microbial organisms include partially pure microbes, substantially pure microbes and microbes cultured in a medium that is non-naturally occurring.
  • microbial As used herein, the terms “microbial,” “microbial organism” or “microorganism” are intended to mean any organism that exists as a microscopic cell that is included within the domains of archaea, bacteria or eukarya. Therefore, the term is intended to encompass prokaryotic or eukaryotic cells or organisms having a microscopic size and includes bacteria, archaea and eubacteria of all species as well as eukaryotic microorganisms such as yeast and fungi. The term also includes cell cultures of any species that can be cultured for the production of a biochemical.
  • CoA or “coenzyme A” is intended to mean an organic cofactor or prosthetic group (nonprotein portion of an enzyme) whose presence is required for the activity of many enzymes (the apoenzyme) to form an active enzyme system.
  • Coenzyme A functions in certain condensing enzymes, acts in acetyl or other acyl group transfer and in fatty acid synthesis and oxidation, pyruvate oxidation and in other acetylation.
  • substantially anaerobic when used in reference to a culture or growth condition is intended to mean that the amount of oxygen is less than about 10% of saturation for dissolved oxygen in liquid media.
  • the term also is intended to include sealed chambers of liquid or solid medium maintained with an atmosphere of less than about 1% oxygen.
  • Exogenous as it is used herein is intended to mean that the referenced molecule or the referenced activity is introduced into the host microbial organism.
  • the molecule can be introduced, for example, by introduction of an encoding nucleic acid into the host genetic material such as by integration into a host chromosome or as non-chromosomal genetic material such as a plasmid. Therefore, the term as it is used in reference to expression of an encoding nucleic acid refers to introduction of the encoding nucleic acid in an expressible form into the microbial organism. When used in reference to a biosynthetic activity, the term refers to an activity that is introduced into the host reference organism.
  • the source can be, for example, a homologous or heterologous encoding nucleic acid that expresses the referenced activity following introduction into the host microbial organism. Therefore, the term “endogenous” refers to a referenced molecule or activity that is present in the host. Similarly, the term when used in reference to expression of an encoding nucleic acid refers to expression of an encoding nucleic acid contained within the microbial organism. The term “heterologous” refers to a molecule or activity derived from a source other than the referenced species whereas “homologous” refers to a molecule or activity derived from the host microbial organism. Accordingly, exogenous expression of an encoding nucleic acid of the invention can utilize either or both a heterologous or homologous encoding nucleic acid.
  • the more than one exogenous nucleic acids refers to the referenced encoding nucleic acid or biosynthetic activity, as discussed above. It is further understood, as disclosed herein, that such more than one exogenous nucleic acids can be introduced into the host microbial organism on separate nucleic acid molecules, on polycistronic nucleic acid molecules, or a combination thereof, and still be considered as more than one exogenous nucleic acid.
  • a microbial organism can be engineered to express two or more exogenous nucleic acids encoding a desired pathway enzyme or protein.
  • two exogenous nucleic acids encoding a desired activity are introduced into a host microbial organism
  • the two exogenous nucleic acids can be introduced as a single nucleic acid, for example, on a single plasmid, on separate plasmids, can be integrated into the host chromosome at a single site or multiple sites, and still be considered as two exogenous nucleic acids.
  • exogenous nucleic acids can be introduced into a host organism in any desired combination, for example, on a single plasmid, on separate plasmids, can be integrated into the host chromosome at a single site or multiple sites, and still be considered as two or more exogenous nucleic acids, for example three exogenous nucleic acids.
  • the number of referenced exogenous nucleic acids or biosynthetic activities refers to the number of encoding nucleic acids or the number of biosynthetic activities, not the number of separate nucleic acids introduced into the host organism.
  • the non-naturally occurring microbal organisms of the invention can contain stable genetic alterations, which refers to microorganisms that can be cultured for greater than five generations without loss of the alteration.
  • stable genetic alterations include modifications that persist greater than 10 generations, particularly stable modifications will persist more than about 25 generations, and more particularly, stable genetic modifications will be greater than 50 generations, including indefinitely.
  • E. coli metabolic modifications are described with reference to a suitable host organism such as E. coli and their corresponding metabolic reactions or a suitable source organism for desired genetic material such as genes for a desired metabolic pathway.
  • a suitable host organism such as E. coli and their corresponding metabolic reactions or a suitable source organism for desired genetic material such as genes for a desired metabolic pathway.
  • desired genetic material such as genes for a desired metabolic pathway.
  • the E. coli metabolic alterations exemplified herein can readily be applied to other species by incorporating the same or analogous encoding nucleic acid from species other than the referenced species.
  • Such genetic alterations include, for example, genetic alterations of species homologs, in general, and in particular, orthologs, paralogs or nonorthologous gene displacements.
  • ortholog is a gene or genes that are related by vertical descent and are responsible for substantially the same or identical functions in different organisms.
  • mouse epoxide hydrolase and human epoxide hydrolase can be considered orthologs for the biological function of hydrolysis of epoxides.
  • Genes are related by vertical descent when, for example, they share sequence similarity of sufficient amount to indicate they are homologous, or related by evolution from a common ancestor.
  • Genes can also be considered orthologs if they share three-dimensional structure but not necessarily sequence similarity, of a sufficient amount to indicate that they have evolved from a common ancestor to the extent that the primary sequence similarity is not identifiable.
  • Genes that are orthologous can encode proteins with sequence similarity of about 25% to 100% amino acid sequence identity. Genes encoding proteins sharing an amino acid similarity less that 25% can also be considered to have arisen by vertical descent if their three-dimensional structure also shows similarities.
  • Orthologs include genes or their encoded gene products that through, for example, evolution, have diverged in structure or overall activity. For example, where one species encodes a gene product exhibiting two functions and where such functions have been separated into distinct genes in a second species, the three genes and their corresponding products are considered to be orthologs. For the production of a biochemical product, those skilled in the art will understand that the orthologous gene harboring the metabolic activity to be introduced or disrupted is to be chosen for construction of the non-naturally occurring microorganism.
  • An example of orthologs exhibiting separable activities is where distinct activities have been separated into distinct gene products between two or more species or within a single species.
  • paralogs are homologs related by, for example, duplication followed by evolutionary divergence and have similar or common, but not identical functions.
  • Paralogs can originate or derive from, for example, the same species or from a different species.
  • microsomal epoxide hydrolase epoxide hydrolase I
  • soluble epoxide hydrolase epoxide hydrolase II
  • Paralogs are proteins from the same species with significant sequence similarity to each other suggesting that they are homologous, or related through co-evolution from a common ancestor.
  • a nonorthologous gene displacement is a nonorthologous gene from one species that can substitute for a referenced gene function in a different species. Substitution includes, for example, being able to perform substantially the same or a similar function in the species of origin compared to the referenced function in the different species.
  • a nonorthologous gene displacement will be identifiable as structurally related to a known gene encoding the referenced function, less structurally related but functionally similar genes and their corresponding gene products nevertheless will still fall within the meaning of the term as it is used herein.
  • Functional similarity requires, for example, at least some structural similarity in the active site or binding region of a nonorthologous gene product compared to a gene encoding the function sought to be substituted. Therefore, a nonorthologous gene includes, for example, a paralog or an unrelated gene.
  • Orthologs, paralogs and nonorthologous gene displacements can be determined by methods well known to those skilled in the art. For example, inspection of nucleic acid or amino acid sequences for two polypeptides will reveal sequence identity and similarities between the compared sequences. Based on such similarities, one skilled in the art can determine if the similarity is sufficiently high to indicate the proteins are related through evolution from a common ancestor. Algorithms well known to those skilled in the art, such as Align, BLAST, Clustal W and others compare and determine a raw sequence similarity or identity, and also determine the presence or significance of gaps in the sequence which can be assigned a weight or score. Such algorithms also are known in the art and are similarly applicable for determining nucleotide sequence similarity or identity.
  • Parameters for sufficient similarity to determine relatedness are computed based on well known methods for calculating statistical similarity, or the chance of finding a similar match in a random polypeptide, and the significance of the match determined.
  • a computer comparison of two or more sequences can, if desired, also be optimized visually by those skilled in the art.
  • Related gene products or proteins can be expected to have a high similarity, for example, 25% to 100% sequence identity. Proteins that are unrelated can have an identity which is essentially the same as would be expected to occur by chance, if a database of sufficient size is scanned (about 5%). Sequences between 5% and 24% may or may not represent sufficient homology to conclude that the compared sequences are related. Additional statistical analysis to determine the significance of such matches given the size of the data set can be carried out to determine the relevance of these sequences.
  • Exemplary parameters for determining relatedness of two or more sequences using the BLAST algorithm can be as set forth below. Briefly, amino acid sequence alignments can be performed using BLASTP version 2.0.8 (Jan. 5, 1999) and the following parameters: Matrix: 0 BLOSUM62; gap open: 11; gap extension: 1; x_dropoff: 50; expect: 10.0; wordsize: 3; filter: on. Nucleic acid sequence alignments can be performed using BLASTN version 2.0.6 (Sep. 16, 1998) and the following parameters: Match: 1; mismatch: ⁇ 2; gap open: 5; gap extension: 2; x_dropoff: 50; expect: 10.0; wordsize: 11; filter: off. Those skilled in the art will know what modifications can be made to the above parameters to either increase or decrease the stringency of the comparison, for example, and determine the relatedness of two or more sequences.
  • a microbial organism that produces an intermediate can be used in combination with another microbial organism expressing downstream pathway enzymes to produce a desired product.
  • a non-naturally occurring microbial organism that produces a butadiene pathway intermediate can be utilized to produce the intermediate as a desired product.
  • This invention is also directed, in part to engineered biosynthetic pathways to improve carbon flux through a central metabolism intermediate en route to butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate.
  • the present invention provides non-naturally occurring microbial organisms having one or more exogenous genes encoding enzymes that can catalyze various enzymatic transformations en route to butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate. In some embodiments, these enzymatic transformations are used to improve product yields, including but not limited to, from carbohydrate-based carbon feedstock.
  • the one or more exogenous genes encoding enzymes that can catalyze various enzymatic transformations en route to butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate can be used in combination with genetic modifications that improve the amount of reducing equivalents available to the biosynthetic pathways, or that minimize loss of reducing equivalents and/or carbon to byproducts.
  • the one or more exogenous genes encoding enzymes that can catalyze various enzymatic transformations en route to butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate can be used in combination with genetic modifications that (i) enhance carbon fixation, and/or (ii) accessing additional reducing equivalents from carbon sources.
  • Reducing equivalents can come in the form of NADH, NADPH, FADH, reduced quinones, reduced ferredoxins, reduced flavodoxins and thioredoxins.
  • the invention is described herein with general reference to the metabolic reaction, reactant or product thereof, or with specific reference to one or more nucleic acids or genes encoding an enzyme associated with or catalyzing, or a protein associated with, the referenced metabolic reaction, reactant or product. Unless otherwise expressly stated herein, those skilled in the art will understand that reference to a reaction also constitutes reference to the reactants and products of the reaction. Similarly, unless otherwise expressly stated herein, reference to a reactant or product also references the reaction, and reference to any of these metabolic constituents also references the gene or genes encoding the enzymes that catalyze or proteins involved in the referenced reaction, reactant or product.
  • reference herein to a gene or encoding nucleic acid also constitutes a reference to the corresponding encoded enzyme and the reaction it catalyzes or a protein associated with the reaction as well as the reactants and products of the reaction.
  • the non-naturally occurring microbial organisms of the invention can be produced by introducing expressible nucleic acids encoding one or more of the enzymes or proteins participating in one or more butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate biosynthetic pathways.
  • nucleic acids for some or all of a particular butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate acid biosynthetic pathway can be expressed.
  • a chosen host is deficient in one or more enzymes or proteins for a desired biosynthetic pathway, then expressible nucleic acids for the deficient enzyme(s) or protein(s) are introduced into the host for subsequent exogenous expression.
  • the chosen host exhibits endogenous expression of some pathway genes, but is deficient in others, then an encoding nucleic acid is needed for the deficient enzyme(s) or protein(s) to achieve butadiene, crotyl alcohol, 3-buten-1-ol, 3-buten-2-ol, and/or 2,4-pentadienoate biosynthesis.
  • a non-naturally occurring microbial organism of the invention can be produced by introducing exogenous enzyme or protein activities to obtain a desired biosynthetic pathway or a desired biosynthetic pathway can be obtained by introducing one or more exogenous enzyme or protein activities that, together with one or more endogenous enzymes or proteins, produces a desired product such as butadiene.
  • Host microbial organisms can be selected from, and the non-naturally occurring microbial organisms generated in, for example, bacteria, yeast, fungus or any of a variety of other microorganisms applicable to fermentation processes.
  • Exemplary bacteria include species selected from Escherichia coli, Klebsiella oxytoca, Anaerobiospirillum succiniciproducens, Actinobacillus succinogenes, Mannheimia succiniciproducens, Rhizobium etli, Bacillus subtilis, Corynebacterium glutamicum, Gluconobacter oxydans, Zymomonas mobilis, Lactococcus lactis, Lactobacillus plantarum, Streptomyces coelicolor, Clostridium acetobutylicum, Pseudomonas fluorescens , and Pseudomonas putida .
  • Exemplary yeasts or fungi include species selected from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis, Kluyveromyces marxianus, Aspergillus terreus, Aspergillus niger, Pichia pastoris, Rhizopus arrhizus, Rhizopus oryzae, Yarrowia lipolytica , and the like.
  • E. coli is a particularly useful host organism since it is a well characterized microbial organism suitable for genetic engineering.
  • Other particularly useful host organisms include yeast such as Saccharomyces cerevisiae . It is understood that any suitable microbial host organism can be used to introduce metabolic and/or genetic modifications to produce a desired product.
  • E. coli host organisms include any non-pathogenic E. coli strain, including E. coli strains falling within taxonomic lineages such as A, B1, and B2.
  • E. coli K-12 strains are in subgroup A.
  • Host organisms include derivatives and variants of E. coli K-12, such as W3110 and MG1655. See, for example, Kuhnert, P., et at. (1995) Rapid and accurate identification of Escherichia coli K-12 strains. Applied and Environmental Microbiology 61:4135-4139; Bachmann, B. J. (1972) Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol. Rev. 36 525-57; and Bachmann, B.
  • a heterologous nucleic acid encoding a pathway enzyme of the disclosure can be described as obtained from an organism (such as a bacteria) that is other than the organisms of the host group.
  • the heterologous nucleic acid can be from a bacterial organism other than an organism selected from the group consisting of E. coli, K. oxytoca, A. succiniciproducens , etc.
  • exogenous expression of the encoding nucleic acids is employed.
  • Exogenous expression confers the ability to custom tailor the expression and/or regulatory elements to the host and application to achieve a desired expression level that is controlled by the user.
  • endogenous expression also can be utilized in other embodiments such as by removing a negative regulatory effector or induction of the gene's promoter when linked to an inducible promoter or other regulatory element.
  • an endogenous gene having a naturally occurring inducible promoter can be up-regulated by providing the appropriate inducing agent, or the regulatory region of an endogenous gene can be engineered to incorporate an inducible regulatory element, thereby allowing the regulation of increased expression of an endogenous gene at a desired time.
  • an inducible promoter can be included as a regulatory element for an exogenous gene introduced into a non-naturally occurring microbial organism.
  • Sources of encoding nucleic acids for a butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol and/or 2,4-pentadienoate pathway enzyme or protein can include, for example, any species where the encoded gene product is capable of catalyzing the referenced reaction.
  • species include both prokaryotic and eukaryotic organisms including, but not limited to, bacteria, including archaea and eubacteria, and eukaryotes, including yeast, plant, insect, animal, and mammal, including human.
  • Exemplary species for such sources include, for example, Escherichia species, including Escherichia coli, Escherichia fergusonii, Methanocaldococcus jannaschii, Leptospira interrrogans, Geobacter sultrreducens, Chloroflexus aurantiacus, Roseiflexus sp.
  • Escherichia species including Escherichia coli, Escherichia fergusonii, Methanocaldococcus jannaschii, Leptospira interrrogans, Geobacter sultrreducens, Chloroflexus aurantiacus, Roseiflexus sp.
  • RS-1 Chloroflexus aggregans, Achromobacter xylosoxydans, Clostrdia species, including Clostridium kluyveri, Clostridium symbiosum, Clostridium acetobutylicum, Clostridium saccharoperbutylacetonicum, Clostridium ljungdahlii, Trichomonas vaginalis G3, Trypanosoma brucei, Acidaminococcus fermentans, Fusobacterium species, including Fusobacterium nucleatum, Fusobacterium mortiferum, Corynebacterium glutamicum, Rattus norvegicus, Homo sapiens, Saccharomyces species, including Saccharomyces cerevisiae, Apsergillus species, including Aspergillus terreus, Aspergillus oryzae, Aspergillus niger, Gibberella zeae, Pichia stipitis, Mycobacterium
  • MR-4 Alcaligenes faecalis, Geobacillus stearothermophilus, Serratia marcescens, Vibrio cholerae, Eubacterium barkeri, Bacteroides capillosus, Archaeoglobus fulgidus, Archaeoglobus fulgidus, Haloarcula marismortui, Pyrobaculum aerophilum str.
  • IM2 Rhizobium species, including Rhizobium leguminosarum , as well as other exemplary species disclosed herein or available as source organisms for corresponding genes.
  • the metabolic alterations allowing biosynthesis of butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate described herein with reference to a particular organism such as E. coli can be readily applied to other microorganisms, including prokaryotic and eukaryotic organisms alike. Given the teachings and guidance provided herein, those skilled in the art will know that a metabolic alteration exemplified in one organism can be applied equally to other organisms.
  • teachings and methods of the invention can be applied to all microbial organisms using the cognate metabolic alterations to those exemplified herein to construct a microbial organism in a species of interest that will synthesize butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol and/or 2,4-pentadienoate.
  • Methods for constructing and testing the expression levels of a non-naturally occurring butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol and/or 2,4-pentadienoate-producing host can be performed, for example, by recombinant and detection methods well known in the art. Such methods can be found described in, for example, Sambrook et al., Molecular Cloning: A Laboratory Manual , Third Ed., Cold Spring Harbor Laboratory, New York (2001); and Ausubel et al., Current Protocols in Molecular Biology , John Wiley and Sons, Baltimore, Md. (1999).
  • Exogenous nucleic acid sequences involved in a pathway for production of butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol and/or 2,4-pentadienoate can be introduced stably or transiently into a host cell using techniques well known in the art including, but not limited to, conjugation, electroporation, chemical transformation, transduction, transfection, and ultrasound transformation.
  • some nucleic acid sequences in the genes or cDNAs of eukaryotic nucleic acids can encode targeting signals such as an N-terminal mitochondrial or other targeting signal, which can be removed before transformation into prokaryotic host cells, if desired.
  • genes can be expressed in the cytosol without the addition of leader sequence, or can be targeted to mitochondrion or other organelles, or targeted for secretion, by the addition of a suitable targeting sequence such as a mitochondrial targeting or secretion signal suitable for the host cells.
  • a suitable targeting sequence such as a mitochondrial targeting or secretion signal suitable for the host cells.
  • An expression vector or vectors can be constructed to include one or more butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate biosynthetic pathway encoding nucleic acids as exemplified herein operably linked to expression control sequences functional in the host organism.
  • Expression vectors applicable for use in the microbial host organisms of the invention include, for example, plasmids, phage vectors, viral vectors, episomes and artificial chromosomes, including vectors and selection sequences or markers operable for stable integration into a host chromosome. Additionally, the expression vectors can include one or more selectable marker genes and appropriate expression control sequences.
  • Selection control sequences can include constitutive and inducible promoters, transcription enhancers, transcription terminators, and the like which are well known in the art.
  • both nucleic acids can be inserted, for example, into a single expression vector or in separate expression vectors.
  • the encoding nucleic acids can be operationally linked to one common expression control sequence or linked to different expression control sequences, such as one inducible promoter and one constitutive promoter.
  • exogenous nucleic acid sequences involved in a metabolic or synthetic pathway can be confirmed using methods well known in the art. Such methods include, for example, nucleic acid analysis such as Northern blots or polymerase chain reaction (PCR) amplification of mRNA, or immunoblotting for expression of gene products, or other suitable analytical methods to test the expression of an introduced nucleic acid sequence or its corresponding gene product. It is understood by those skilled in the art that the exogenous nucleic acid is expressed in a sufficient amount to produce the desired product, and it is further understood that expression levels can be optimized to obtain sufficient expression using methods well known in the art and as disclosed herein.
  • the invention additionally provides methods of producing butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate using the microbial organisms of the invention comprising one or more pathway gene(s).
  • the invention provides a method for producing a target compound by culturing a non-naturally occurring microbial organism, comprising a microbial organism having a butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate pathway comprising at least one exogenous nucleic acid encoding a pathway enzyme expressed in a sufficient amount to produce butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate, under conditions and for a sufficient period of time.
  • Suitable purification and/or assays to test for the production of producing butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate can be performed using well known methods. Suitable replicates such as triplicate cultures can be grown for each engineered strain to be tested. For example, product and byproduct formation in the engineered production host can be monitored. The final product and intermediates, and other organic compounds, can be analyzed by methods such as HPLC (High Performance Liquid Chromatography), GC-MS (Gas Chromatography-Mass Spectroscopy) and LC-MS (Liquid Chromatography-Mass Spectroscopy) or other suitable analytical methods using routine procedures well known in the art.
  • HPLC High Performance Liquid Chromatography
  • GC-MS Gas Chromatography-Mass Spectroscopy
  • LC-MS Liquid Chromatography-Mass Spectroscopy
  • the release of product in the fermentation broth can also be tested with the culture supernatant.
  • Byproducts and residual glucose can be quantified by HPLC using, for example, a refractive index detector for glucose and alcohols, and a UV detector for organic acids (Lin et al., Biotechnol. Bioeng. 90:775-779 (2005)), or other suitable assay and detection methods well known in the art.
  • the individual enzyme or protein activities from the exogenous DNA sequences can also be assayed using methods well known in the art.
  • the butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate can be separated from other components in the culture using a variety of methods well known in the art.
  • separation methods include, for example, extraction procedures as well as methods that include continuous liquid-liquid extraction, pervaporation, membrane filtration, membrane separation, reverse osmosis, electrodialysis, distillation, crystallization, centrifugation, extractive filtration, ion exchange chromatography, size exclusion chromatography, adsorption chromatography, and ultrafiltration. All of the above methods are well known in the art.
  • any of the non-naturally occurring microbial organisms described herein can be cultured to produce and/or secrete the biosynthetic products of the invention.
  • the butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate producers can be cultured for the biosynthetic production of butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate.
  • the recombinant strains are cultured in a medium with carbon source and other essential nutrients. It is sometimes desirable and can be highly desirable to maintain anaerobic conditions in the fermenter to reduce the cost of the overall process. Such conditions can be obtained, for example, by first sparging the medium with nitrogen and then sealing the flasks with a septum and crimp-cap. For strains where growth is not observed anaerobically, microaerobic or substantially anaerobic conditions can be applied by perforating the septum with a small hole for limited aeration.
  • Exemplary anaerobic conditions have been described previously and are well-known in the art. Exemplary aerobic and anaerobic conditions are described, for example, in United State publication 2009/0047719, filed Aug. 10, 2007. Fermentations can be performed in a batch, fed-batch or continuous manner, as disclosed herein.
  • the pH of the medium can be maintained at a desired pH, in particular neutral pH, such as a pH of around 7 by addition of a base, such as NaOH or other bases, or acid, as needed to maintain the culture medium at a desirable pH.
  • the growth rate can be determined by measuring optical density using a spectrophotometer (600 nm), and the glucose uptake rate by monitoring carbon source depletion over time.
  • the growth medium can include, for example, any carbohydrate source which can supply a source of carbon to the non-naturally occurring microorganism.
  • Such sources include, for example, sugars such as glucose, xylose, arabinose, galactose, mannose, fructose, sucrose and starch.
  • Other sources of carbohydrate include, for example, renewable feedstocks and biomass.
  • Exemplary types of biomasses that can be used as feedstocks in the methods of the invention include cellulosic biomass, hemicellulosic biomass and lignin feedstocks or portions of feedstocks.
  • Such biomass feedstocks contain, for example, carbohydrate substrates useful as carbon sources such as glucose, xylose, arabinose, galactose, mannose, fructose and starch.
  • Other non-carbohydrate feedstocks include alcohols such as methanol, ethanol and glycerol and gaseous carbon substrates such as methane and syngas.
  • renewable feedstocks and biomass other than those exemplified above also can be used for culturing the microbial organisms of the invention for the production of butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate.
  • culture conditions include anaerobic or substantially anaerobic growth or maintenance conditions.
  • Exemplary anaerobic conditions have been described previously and are well known in the art.
  • Exemplary anaerobic conditions for fermentation processes are described herein and are described, for example, in U.S. publication 2009/0047719, filed Aug. 10, 2007. Any of these conditions can be employed with the non-naturally occurring microbial organisms as well as other anaerobic conditions well known in the art.
  • butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate producers can synthesize butadiene, crotyl alcohol, 3-buten-1-ol, 3-buten-2-ol, and/or 2,4-pentadienoate at intracellular concentrations of 5-10 mM or more as well as all other concentrations exemplified herein.
  • butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate producing microbial organisms can butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate intracellularly and/or secrete the product into the culture medium.
  • growth condition for achieving biosynthesis of butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate can include the addition of an osmoprotectant to the culturing conditions.
  • the non-naturally occurring microbial organisms of the invention can be sustained, cultured or fermented as described herein in the presence of an osmoprotectant.
  • an osmoprotectant refers to a compound that acts as an osmolyte and helps a microbial organism as described herein survive osmotic stress.
  • Osmoprotectants include, but are not limited to, betaines, amino acids, and the sugar trehalose.
  • Non-limiting examples of such are glycine betaine, proline betaine, dimethylthetin, dimethylsulfoniopropionate, 3-dimethylsulfonio-2-methylpropionate, pipecolic acid, dimethylsulfonioacetate, choline, L-carnitine and ectoine.
  • the osmoprotectant is glycine betaine.
  • the carbon feedstock and other cellular uptake sources such as phosphate, ammonia, sulfate, chloride and other halogens can be chosen to alter the isotopic distribution of the atoms present in butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate or any pathway intermediate.
  • uptake sources The various carbon feedstock and other uptake sources enumerated above will be referred to herein, collectively, as “uptake sources.”
  • bioderived means derived from or synthesized by a biological organism and can be considered a renewable resource since it can be generated by a biological organism.
  • a biological organism in particular the microbial organisms of the invention disclosed herein, can utilize feedstock or biomass, such as, sugars or carbohydrates obtained from an agricultural, plant, bacterial, or animal source.
  • the biological organism can utilize atmospheric carbon.
  • biobased means a product as described above that is composed, in whole or in part, of a bioderived compound of the invention.
  • a biobased or bioderived product is in contrast to a petroleum derived product, wherein such a product is derived from or synthesized from petroleum or a petrochemical feedstock.
  • the culture conditions can include, for example, liquid culture procedures as well as fermentation and other large scale culture procedures. As described herein, particularly useful yields of the biosynthetic products of the invention can be obtained under anaerobic or substantially anaerobic culture conditions.
  • one exemplary growth condition for achieving biosynthesis of butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate includes anaerobic culture or fermentation conditions.
  • the non-naturally occurring microbial organisms of the invention can be sustained, cultured or fermented under anaerobic or substantially anaerobic conditions.
  • Anaerobic conditions refer to an environment devoid of oxygen.
  • the culture conditions described herein can be scaled up and grown continuously for manufacturing of butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate.
  • Exemplary growth procedures include, for example, fed-batch fermentation and batch separation; fed-batch fermentation and continuous separation, or continuous fermentation and continuous separation.
  • FIGS. 1-2 Several pathways are shown in FIGS. 1-2 for converting pyruvate and acetaldehyde to butadiene and butadiene precursors.
  • Acetaldehyde is formed via reduction of acetyl-CoA by acetyl-CoA reductase, also called acetaldehyde dehydrogenase (Step 1A and 2A). Pyruvate and acetaldehyde are condensed to 4-hydroxy-2-oxovalerate by 4-hydroxy-2-ketovalerate aldolase (Step 1B and 2B).
  • the acetaldehyde intermediate can be formed by other enzymes or metabolic pathways known in the art, such as pyruvate decarboxylase.
  • Steps A and B of FIGS. 1 and 2 are catalyzed by a bifunctional enzyme with aldolase and dehydrogenase activities.
  • the 4-hydroxy-2-oxovalerate product is subsequently dehydrated to 2-oxopent-3-enoate (Step 1E).
  • Step 1F Reduction of 2-oxopent-3-enoate to its corresponding hydroxyacid (Step 1F) is catalyzed by a secondary alcohol dehydrogenase.
  • Isomerization of 2-hydroxypent-3-enoate to 4-hydroxypent-2-enoate (1U), followed by dehydration (1V) yields 2,4-pentadienoate.
  • These two reactions can be catalyzed by two different enzymes (1U, 1V) or by a bifunctional isomerase/dehydratase (1G).
  • Decarboxylation of 2,4-pentadienoate to butadiene is catalyzed by a pentadienoate decarboxylase (1H).
  • the conversion of the 2-oxopent-3-enoate or 2-hydroxypent-3-enoate intermediates to 2,4-pentadienoate proceeds via acyl-CoA intermediates (Steps 1N, 1O, 1P, 1Q, 1R).
  • the conversion of an acid to an acyl-CoA is catalyzed by CoA transferases and CoA synthetases.
  • Acyl-CoA to acid conversion is catalyzed by CoA synthetases, transferases or hydrolases.
  • FIG. 2 also shows pathways derived from the 4-hydroxy 2-oxovalerate intermediate.
  • 4-hydroxy 2-oxovalerate is dehydrated to 2-oxopent-4-enoate (also called 2-hydroxypenta-2,4-dienoate) by 4-hydroxy-2-oxopentanoate dehydratase, also called 2-oxopent-4-enoate hydratase (2C).
  • An alcohol dehydrogenase with 2-oxopent-4-enoate reductase activity forms 2-hydroxypent-4-enoate.
  • Multiple enzymatic routes are shown in FIG. 2 for converting 2-hydroxypent-4-enoate to butadiene and butadiene precursors.
  • One route entails isomerization of 2-hydroxypent-4-enoate to 5-hydroxypent-2-enoate (2E). This intermediate is then decarboxylated to 3-buten-1-ol (2P) and dehydrated to butadiene (2Q). Alternately, the 5-hydroxypent-2-enoate is further dehydrated to pentadienoate (2F) and subsequently decarboxylated to butadiene (2G). In yet another pathway, the 5-hydroxypent-2-enoate intermediate is activated to its corresponding acyl-CoA (2I), dehydrated to pentadienoyl-CoA (2N), and further hydrolyzed to 2,4-pentadienoate (2J).
  • Conversion of 3-HPE-CoA to 2,4-pentadienoyl-CoA is catalyzed by an acyl-CoA dehydratase (2O) or an isomerase and dehydratase (2M, 2N).
  • Useful products shown in FIG. 2 include butadiene, 3-buten-1-ol, 4-hydroxy-2-oxovalerate, 2-oxopent-4-enoate, 2-hydroxypent-4-enoate, 4-hydroxypent-2-enoate, 3HPE and 2,4-pentadienoate. Enzymes for catalyzing each step are described below.
  • FIG. 3 Pathways converting lactoyl-CoA to 4-hydroxypent-2-enoate, and further to 3-buten-2-ol and butadiene, are shown in FIG. 3 .
  • the conversion of lactoyl-CoA to 4-hydroxypent-2-enoate is accomplished in four or more enzymatic steps shown in FIG. 3 .
  • Lactoyl-CoA and acetyl-CoA are first condensed to 3-oxo-4-hydroxypentanoyl-CoA by 3-oxo-4-hydroxypentanoyl-CoA thiolase, a beta-ketothiolase (Step 3A).
  • the 3-oxo-4-hydroxypentanoyl-CoA intermediate is converted to its corresponding acid by a CoA hydrolase, transferase or synthetase (3B).
  • Reduction of the 3-oxo ketone by an alcohol dehydrogenase yields 3,4-dihydroxypentanoate (3C).
  • Dehydration of the dihydroxyacid yields 4-hydroxypent-2-enoate (3D).
  • 4-Hydroxypent-2-enoate decarboxylase converts 4-hydroxypent-2-enoate to 3-buten-2-ol (3E). Isomerization of 3-buten-2-ol to butadiene is catalyzed by a vinylisomerase (3F).
  • Decarboxylation and dehydration of 4-hydroxypent-2-enoate to butadiene can instead proceed with dehydration first (3K) followed by decarboxylation of 2,4-pentadienoate (3L).
  • Further alternate pathways shown in FIG. 3 entail reduction of the 3-oxo-4-hydroxypentanoyl-CoA to its 3-hydroxyacyl-CoA (3G), and optional dehydration of the 3-hydroxyacyl-CoA to 4-hydroxypent-2-enoyl-CoA (3H).
  • Conversion of the acyl-CoA intermediates to their corresponding acids by CoA hydrolases, synthases and transferases is shown in steps 3I and 3J.
  • FIG. 4 shows enzymatic pathways for converting CrotOH to butadiene.
  • CrotOH is phosphorylated to 2-butenyl-4-phosphate by a CrotOH kinase (Step A).
  • the 2-butenyl-4-phosphate intermediate is again phosphorylated to 2-butenyl-4-diphosphate (Step B).
  • a butadiene synthase (BDS) enzyme catalyzes the conversion of 2-butenyl-4-diphosphate to butadiene (Step C).
  • BDS butadiene synthase
  • Such a BDS can be derived from a phosphate lyase enzyme such as isoprene synthase using methods, such as directed evolution, as described herein.
  • CrotOH is directly converted to 2-butenyl-4-diphosphate by a diphosphokinase (step I).
  • CrotOH can be converted to butadiene by a CrotOH dehydratase or a bifunctional dehydratase/isomerase (step H).
  • the 2-butenyl-4-phosphate intermediate is directly converted to butadiene by a BDS (monophosphate) (step J). Further are shown pathways that proceed through a 3-buten-2-ol (MVC) intermediate.
  • Crotyl alcohol is isomerized to MC by an enzyme with vinylisomerase activity (step F).
  • 3-Buten-2-ol synthase enzymes catalyze the conversion of 2-butenyl-4-phosphate or 2-butenyl-4-diphosphate to 3-buten-2-ol (Steps 4D and 4E, respectively).
  • the 3-buten-2-ol intermediate is then dehydrated to butadiene (4G).
  • the enzyme activities required for the reactions shown in FIGS. 1-4 are listed in the table and described in further detail below.
  • the reduction of 2-oxopent-3-enoate, 2-oxopent-4-enoate to corresponding 2-hydroxyacids (1F, 2D) are catalyzed by secondary alcohol dehydrogenases with 2-ketoacid reductase activity.
  • Exemplary secondary alcohol dehydrogenases include malate dehydrogenase, lactate dehydrogenase, 2-ketoadipate reductase, isopropanol dehydrogenase, methyl ethyl ketone reductase, and others described below and known in the art.
  • Two secondary alcohol dehydrogenase enzymes from E are described below and known in the art.
  • malate dehydrogenase EC 1.1.1.37, 1.1.1.82, 1.1.1.299
  • lactate dehydrogenase ldhA
  • S. cerevisiae encodes three copies of malate dehydrogenase, MDH1 (McAlister-Henn and Thompson, J. Bacteriol. 169:5157-5166 (1987), MDH2 (Minard and McAlister-Henn, Mol. Cell. Biol. 11:370-380 (1991); Gibson and McAlister-Henn, J. Biol. Chem. 278:25628-25636 (2003)), and MDH3 (Steffan and McAlister-Henn, J. Biol. Chem.
  • alpha-ketoadipate into alpha-hydroxyadipate can be catalyzed by 2-ketoadipate reductase, an enzyme reported to be found in rat and in human placenta (Suda et al., Arch. Biochem. Biophys. 176:610-620 (1976); Suda et al., Biochem. Biophys. Res. Commun. 77:586-591 (1977)).
  • Alcohol dehydrogenase enzymes of C. beijerinckii Ismaiel et al., J. Bacteriol. 175:5097-5105 (1993)
  • T. brockii Lamed et al., Biochem. J.
  • Methyl ethyl ketone reductase catalyzes the reduction of MEK to 2-butanol.
  • Exemplary MEK reductase enzymes can be found in Rhodococcus ruber (Kosjek et al., Biotechnol Bioeng. 86:55-62 (2004)) and Pyrococcus furiosus (van der Oost et al., Eur. J. Biochem. 268:3062-3068 (2001)).
  • Rhizobium Sinorhizobium
  • meliloti 3-hydroxybutyrate as a carbon source
  • Additional candidates can be found in Pseudomonas fragi (Ito et al., J. Mol. Biol. 355(4) 722-733 (2006)) and Ralstonia pickettii (Takanashi et al., Antonie van Leeuwenoek, 95(3):249-262 (2009)).
  • 3-hydroxybutyrate dehydrogenase EC 1.1.1.30
  • 3-hydroxyisobutyrate dehydrogenase EC 1.1.1.31
  • threonine dehydrogenase EC 1.1.1.103
  • 3-hydroxypropionate dehydrogenase EC 1.1.1.298
  • Alcohol dehydrogenase enzymes active on allyl alcohols are suitable for reducing crotyl aldehyde to crotyl alcohol (1J). Crotyl aldehyde reductase activity has been demonstrated by mdr of Synechocystis sp. PCC 6803 (Shimakawa et al, Biosci Biotechnol Biochem 77:2441-8 (2013)).
  • An exemplary allyl alcohol dehydrogenase is the NtRed-1 enzyme from Nicotiana tabacum (Matsushima et al, Bioorg Chem 36: 23-8 (2008)).
  • a similar enzyme has been characterized in Pseudomonas putida MB1 but the enzyme has not been associated with a gene to date (Malone et al, AEM 65: 2622-30 (1999)).
  • Yet another allyl alcohol dehydrogenase is the geraniol dehydrogenase enzymes of Castellaniella defragrans, Carpoglyphus lactis and Ocimum basilicum (Lueddeke et al, AEM 78:2128-36 (2012)).
  • Alcohol dehydrogenase enzymes with broad substrate specificity are also applicable here, such as include alrA encoding a medium-chain alcohol dehydrogenase for C2-C14 (Tani et al., Appl.
  • coli catalyzes the reduction of a wide range of aldehydes using NADPH as the cofactor, with a preference for chain lengths longer than C(3) (Sulzenbacher et al, J Mol Biol 342:489-502 (2004); Perez et al., J Biol. Chem. 283:7346-7353 (2008)).
  • the adhA gene product from Zymomonas mobilis has been demonstrated to have activity on a number of aldehydes including formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde, and acrolein (Kinoshita et al., Appl Microbiol Biotechnol 22:249-254 (1985)).
  • Alcohol dehydrogenases active on 3-hydroxyacyl-CoA and 2-hydroxyacyl-CoA substrates catalyze the reduction of 2-oxopent-3-enoyl-CoA ( FIG. 1O ) and 3-oxo-4-hydroxypentanoyl-CoA ( FIG. 3G ) to their corresponding hydroxyacyl-CoA products.
  • 3-Oxoacyl-CoA reductase enzymes (EC 1.1.1.35) convert 3-oxoacyl-CoA molecules into 3-hydroxyacyl-CoA molecules and are often involved in fatty acid beta-oxidation or phenylacetate catabolism. For example, subunits of two fatty acid oxidation complexes in E.
  • coli encoded by fadB and fadJ, function as 3-hydroxyacyl-CoA dehydrogenases (Binstock et al., Methods Enzymol. 71 Pt C:403-411 (1981)).
  • the paaH gene product has a similar activity (Nogales et al., 153:357-365 (2007)).
  • Additional 3-oxoacyl-CoA enzymes include the gene products of phaC in Pseudomonas putida (Olivera et al., Proc. Natl. Acad. Sci.
  • An exemplary 2-oxoacyl-CoA reductase is the 3-hydroxy-2-methylbutyryl-CoA dehydrogenase (EC 1.1.1.178) of Pseudomonas putida , which catalyzes the reduction of 3-methyl-2-oxopentanoyl-CoA, in addition to its native activity (Conrad et al, J Bacteriol 118:103-11 (1974)).
  • Acetyl-CoA reductase catalyzes the reduction of acetyl-CoA to acetaldehyde.
  • acyl-CoA dehydrogenases reduce an acyl-CoA to its corresponding aldehyde and represent suitable enzyme candidates for catalyzing step A of FIGS. 1 and 2 .
  • the NAD(P)H dependent reduction of acetyl-CoA to acetaldehyde is catalyzed by acylating acetaldehyde dehydrogenase (EC 1.2.1.10).
  • coli are encoded by adhE and mhpF (Ferrandez et al, J Bacteriol 179:2573-81 (1997)).
  • the Pseudomonas sp. CF600 enzyme encoded by dmpF, participates in meta-cleavage pathways and forms a complex with 4-hydroxy-2-oxovalerate aldolase (Shingler et al, J Bacteriol 174:711-24 (1992)).
  • Solventogenic organisms such as Clostridium acetobutylicum encode bifunctional enzymes with alcohol dehydrogenase and acetaldehyde dehydrogenase activities. The bifunctional C.
  • acetobutylicum enzymes are encoded by bdh I and adhE2 (Walter, et al., J. Bacteriol. 174:7149-7158 (1992); Fontaine et al., J. Bacteriol. 184:821-830 (2002)).
  • Yet another candidate for acylating acetaldehyde dehydrogenase is the ald gene from Clostridium beijerinckii (Toth, Appl. Environ. Microbiol. 65:4973-4980 (1999). This gene is very similar to the eutE acetaldehyde dehydrogenase genes of Salmonella typhimurium and E. coli (Toth, Appl. Environ. Microbiol.
  • exemplary enzymes with acetyl-CoA reductase activity are found in the EC 1.2.1.-enzyme class including fatty acyl-CoA reductase, succinyl-CoA reductase (EC 1.2.1.76), acetyl-CoA reductase, butyryl-CoA reductase and propionyl-CoA reductase (EC 1.2.1.3).
  • Such enzymes include bphG of Pseudomonas sp (Powlowski, J. Bacteriol.
  • Beta-ketothiolase enzymes are required for the conversion of lactoyl-CoA and acetyl-CoA to 3-oxo-4-hydroxypentanoyl-CoA, shown in FIG. 3A .
  • Suitable enzymes are found in EC class 2.3.1, and include beta-ketovaleryl-CoA thiolase, acetoacetyl-CoA thiolase and beta-ketoadipyl-CoA thiolase.
  • Beta-ketovaleryl-CoA thiolase catalyzes the formation of beta-ketovalerate from acetyl-CoA and propionyl-CoA.
  • Zoogloea ramigera possesses two ketothiolases that can form beta-ketovaleryl-CoA from propionyl-CoA and acetyl-CoA and R. eutropha has a beta-oxidation ketothiolase that is also capable of catalyzing this transformation (Gruys et al., U.S. Pat. No. 5,958,745).
  • the sequences of these genes or their translated proteins have not been reported, but several genes in R. eutropha, Z. ramigera , or other organisms can be identified based on sequence homology to bktB from R. eutropha .
  • Acetoacetyl-CoA thiolase converts two molecules of acetyl-CoA into acetoacetyl-CoA (EC 2.1.3.9).
  • Exemplary acetoacetyl-CoA thiolase enzymes include the gene products of atoB from E. coli (Martin et al., Nat. Biotechnol. 21:796-802 (2003)), thlA and thlB from C. acetobutylicum (Hanai et al., Appl. Environ. Microbiol. 73:7814-7818 (2007); Winzer et al., J. Mol. Microbiol. Biotechnol. 2:531-541 (2000)), and ERG10 from S.
  • Beta-ketoadipyl-CoA thiolase (EC 2.3.1.174), also called 3-oxoadipyl-CoA thiolase, converts beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA, and is a key enzyme of the beta-ketoadipate pathway for aromatic compound degradation.
  • the enzyme is widespread in soil bacteria and fungi including Pseudomonas putida (Harwood et al., J. Bacteriol.
  • the P. putida enzyme is a homotetramer bearing 45% sequence homology to beta-ketothiolases involved in PHB synthesis in Ralstonia eutropha , fatty acid degradation by human mitochondria and butyrate production by Clostridium acetobutylicum (Harwood et al., supra).
  • a beta-ketoadipyl-CoA thiolase in Pseudomonas knackmussii (formerly sp. B13) has also been characterized (Gobel et al., J. Bacteriol. 184:216-223 (2002); Kaschabek et al., supra). BKT encoding genes and associated identifiers are shown in the table below.
  • CrotOH kinase enzymes catalyze the transfer of a phosphate group to the hydroxyl group of CrotOH, shown in step A of FIG. 4 .
  • the enzymes described below naturally possess such activity or can be engineered to exhibit this activity.
  • Kinases that catalyze transfer of a phosphate group to an alcohol group are members of the EC 2.7.1 enzyme class.
  • the table below lists several useful kinase enzymes in the EC 2.7.1 enzyme class.
  • Mevalonate kinase (EC 2.7.1.36) phosphorylates the terminal hydroxyl group of mevalonate.
  • Gene candidates for this step include erg12 from S. cerevisiae , mvk from Methanocaldococcus jannaschi , MVK from Homo sapeins , and mvk from Arabidopsis thaliana col.
  • Additional mevalonate kinase candidates include the feedback-resistant mevalonate kinase from the archeon Methanosarcina mazei (Primak et al, AEM , in press (2011)) and the Mvk protein from Streptococcus pneumoniae (Andreassi et al, Protein Sci, 16:983-9 (2007)).
  • Mvk proteins from S. cerevisiae, S. pneumoniae and M. mazei were heterologously expressed and characterized in E. coli (Primak et al, supra).
  • the S. pneumoniae mevalonate kinase was active on several alternate substrates including cylopropylmevalonate, vinylmevalonate and ethynylmevalonate (Kudoh et al, Bioorg Med Chem 18:1124-34 (2010)), and a subsequent study determined that the ligand binding site is selective for compact, electron-rich C(3)-substituents (Lefurgy et al, J Biol Chem 285:20654-63 (2010)).
  • Glycerol kinase also phosphorylates the terminal hydroxyl group in glycerol to form glycerol-3-phosphate. This reaction occurs in several species, including Escherichia coli, Saccharomyces cerevisiae , and Thermotoga maritima .
  • Escherichia coli Saccharomyces cerevisiae
  • Thermotoga maritima The E. coli glycerol kinase has been shown to accept alternate substrates such as dihydroxyacetone and glyceraldehyde (Hayashi et al., J Biol. Chem. 242:1030-1035 (1967)).
  • T maritime has two glycerol kinases (Nelson et al., Nature 399:323-329 (1999)).
  • Glycerol kinases have been shown to have a wide range of substrate specificity. Crans and Whiteside studied glycerol kinases from four different organisms ( Escherichia coli, S. cerevisiae, Bacillus stearothermophilus , and Candida mycoderma ) (Crans et al., J. Am. Chem. Soc. 107:7008-7018 (2010); Nelson et al., supra, (1999)). They studied 66 different analogs of glycerol and concluded that the enzyme could accept a range of substituents in place of one terminal hydroxyl group and that the hydrogen atom at C2 could be replaced by a methyl group. Interestingly, the kinetic constants of the enzyme from all four organisms were very similar.
  • Homoserine kinase is another possible candidate. This enzyme is also present in a number of organisms including E. coli, Streptomyces sp, and S. cerevisiae . Homoserine kinase from E. coli has been shown to have activity on numerous substrates, including, L-2-amino,1,4-butanediol, aspartate semialdehyde, and 2-amino-5-hydroxyvalerate (Huo et al., Biochemistry 35:16180-16185 (1996); Huo et al., Arch. Biochem. Biophys. 330:373-379 (1996)). This enzyme can act on substrates where the carboxyl group at the alpha position has been replaced by an ester or by a hydroxymethyl group.
  • FIG. 8 is a graph of in vitro formation of crotyl phosphate (CrPi) and ADP over time from a composition including ATP, crotyl alcohol, and hydroxyethylthiazole kinase), thiamine kinase, pantothenate kinase, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, riboflavin kinase, L-fuculokinase and choline kinase. Exemplary gene candidates for each of these classes are shown below.
  • 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase can also carry the described transformation. Gene candidates from this class are listed below.
  • Pantothenate kinases that can catalyze the transformation are:
  • Yet another candidate enzyme class of interest is 4-(cytidine 5′-diphospho)-2-C-methyl-D-erythritol kinase (2.7.1.148).
  • Gene candidates from this class are:
  • 2-Butenyl-4-phosphate kinase enzymes catalyze the transfer of a phosphate group to the phosphate group of 2-butenyl-4-phosphate ( FIG. 4B ).
  • the enzymes described below naturally possess such activity or can be engineered to exhibit this activity.
  • Kinases that catalyze transfer of a phosphate group to another phosphate group are members of the EC 2.7.4 enzyme class.
  • Phosphomevalonate kinase enzymes are of particular interest.
  • Phosphomevalonate kinase (EC 2.7.4.2) catalyzes the analogous transformation to 2-butenyl-4-phosphate kinase.
  • This enzyme is encoded by erg8 in Saccharomyces cerevisiae (Tsay et al., Mol. Cell Biol. 11:620-631 (1991)) and mvaK2 in Streptococcus pneumoniae, Staphylococcus aureus and Enterococcus faecalis (Doun et al., Protein Sci. 14:1134-1139 (2005); Wilding et al., J Bacteriol. 182:4319-4327 (2000)).
  • the Streptococcus pneumoniae and Enterococcus faecalis enzymes were cloned and characterized in E. coli (Pilloff et al., J Biol. Chem. 278:4510-4515 (2003); Doun et al., Protein Sci. 14:1134-1139 (2005)).
  • the S. pneumoniae phosphomevalonate kinase was active on several alternate substrates including cylopropylmevalonate phosphate, vinylmevalonate phosphate and ethynylmevalonate phosphate (Kudoh et al, Bioorg Med Chem 18:1124-34 (2010)). These and related enzymes are shown in the table below.
  • Corynebacterium terpenotabidum kinase Y-11 isopentenyl phosphate NP_247007.1 15668214
  • Methanocaldococcus jannaschii kinase isopentenyl phosphate NP_393581.1 16081271
  • Thermoplasma acidophilum DSM kinase 1728 isopentenyl phosphate NP_275190.1 15678076
  • Methanothermobacter kinase thermautotrophicus isopentenyl phosphate YP_003356693.1 282164308 Methanocella paludicola SANAE kinase isopentenyl phosphate YP_3049
  • Additional kinase enzymes include fosfomycin kinase (FomA) which is highly homologous to isopentenyl phosphate kinase and is an antibiotic resistance enzyme found in a few strains of Streptomyces and Pseudomonas (Mabangalo et al. Biochemistry 51(4):917-925 (2012)).
  • FomA Thermoplasma acidophilum
  • IPK and FomA structures aligns their respective substrates and catalytic residues. These residues are conserved only in the IPK and FomA members of the phosphate subdivision of the amino acid kinase superfamily.
  • IPK from Thermoplasma acidophilum has been shown to have activity on fosmomycin.
  • An exemplary fosfomycin kinase is that from Streptomyces wedmorensis , Genbank ID BAA32493.1 and GI number 3452580.
  • Farnesyl monophosphate kinase enzymes catalyze the CTP dependent phosphorylation of farnesyl monophosphate to farnesyl diphosphate.
  • geranylgeranyl phosphate kinase catalyzes CTP dependent phosphorylation. Enzymes with these activities were identified in the microsomal fraction of cultured Nicotiana tabacum (Thai et al, PNAS 96:13080-5 (1999)). However, the associated genes have not been identified to date.
  • Additional enzymes include those of the EC 2.7.2.8 class. This class is exemplified by acetylglutamate kinase, including the exemplary enzymes below:
  • CrotOH diphosphokinase enzymes catalyze the transfer of a diphosphate group to the hydroxyl group of CrotOH, shown in step I of FIG. 1 .
  • the enzymes described below naturally possess such activity or can be engineered to exhibit this activity.
  • Kinases that catalyze transfer of a diphosphate group are members of the EC 2.7.6 enzyme class.
  • the table below lists several useful kinase enzymes in the EC 2.7.6 enzyme class.
  • ribose-phosphate diphosphokinase enzymes which have been identified in Escherichia coli (Hove-Jenson et al., J Biol Chem, 1986, 261(15); 6765-71) and Mycoplasma pneumoniae M129 (McElwain et al, International Journal of Systematic Bacteriology, 1988, 38:417-423) as well as thiamine diphosphokinase enzymes.
  • Exemplary thiamine diphosphokinase enzymes are found in Arabidopsis thaliana (Ajjawi, Plant Mol Biol, 2007, 65(1-2); 151-62).
  • Olefin-forming decarboxylase enzymes suitable for converting 3-hydroxypent-4-enoate to butadiene include mevalonate diphosphate decarboxylase (MDD, EC 4.1.1.33) and similar enzymes. MDD participates in the mevalonate pathway for isoprenoid biosynthesis, where it catalyzes the ATP-dependent decarboxylation of mevalonate diphosphate to isopentenyl diphosphate. The MDD enzyme of S. cerevisiae was heterologously expressed in E.
  • MDD genes are MVD in Homo sapiens and MDD in Staphylococcus aureus and Trypsonoma brucei (Toth et al., J Biol. Chem. 271:7895-7898 (1996); Byres et al., J Mol. Biol. 371:540-553 (2007)).
  • Step B of FIGS. 1 and 2 The condensation of pyruvate and acetaldehyde to 4-hydroxy-2-oxovalerate (Step B of FIGS. 1 and 2 ) is catalyzed by 4-hydroxy-2-oxovalerate aldolase (EC 4.1.3.39). This enzyme participates in pathways for the degradation of phenols, cresols and catechols.
  • the E. coli enzyme, encoded by mhpE, is highly specific for acetaldehyde as an acceptor (Pollard et al., Appl Environ Microbiol 64:4093-4094 (1998)).
  • Substrate channeling provides the advantage of reduced cellular concentrations of acetaldehyde, toxic to some cells, and may also reduce acetaldehyde-derived byproducts such as ethanol and acetate.
  • a similar aldolase-dehydrogenase complex is encoded by BphIJ of Burkholderia xenovorans (Baker et al, Biochem 48:6551-8 (2009)).
  • Butadiene Synthase (BDS), shown in Step C of FIG. 4 , catalyzes the conversion of 2-butenyl-4-diphosphate to 1,3-butadiene.
  • the enzymes described below naturally possess such activity or can be engineered to exhibit this activity.
  • Carbon-oxygen lyases that operate on phosphates are found in the EC 4.2.3 enzyme class.
  • the table below lists several useful enzymes in EC class 4.2.3.
  • enzymes include isoprene synthase, myrcene synthase and farnesene synthase. Enzyme candidates are described below, and in the enzymes and classes for FIG. 15 , Step F.
  • Isoprene synthase naturally catalyzes the conversion of dimethylallyl diphosphate to isoprene, but can also catalyze the synthesis of 1,3-butadiene from 2-butenyl-4-diphosphate.
  • Isoprene synthases can be found in several organisms including Populus alba (Sasaki et al., FEBS Letters, 2005, 579 (11), 2514-2518), Pueraria montana (Lindberg et al., Metabolic Eng, 12(1):70-79 (2010); Sharkey et al., Plant Physiol., 137(2):700-712 (2005)), and Populus tremula x Populus alba , also called Populus canescens (Miller et al., Planta, 2001, 213 (3), 483-487).
  • Myrcene synthase enzymes catalyze the dephosphorylation of geranyl diphosphate to beta-myrcene (EC 4.2.3.15).
  • Exemplary myrcene synthases are encoded by MST2 of Solanum lycopersicum (van Schie et al, Plant Mol Biol 64:D473-79 (2007)), TPS-Myr of Picea abies (Martin et al, Plant Physiol 135:1908-27 (2004)) g-myr of Abies grandis (Bohlmann et al, J Biol Chem 272:21784-92 (1997)) and TPS10 of Arabidopsis thaliana (Bohlmann et al, Arch Biochem Biophys 375:261-9 (2000)). These enzymes were heterologously expressed in E. coli .
  • Farnesyl diphosphate is converted to alpha-farnesene and beta-farnesene by alpha-farnesene synthase and beta-farnesene synthase, respectively.
  • alpha-farnesene synthase enzymes include TPS03 and TPS02 of Arabidopsis thaliana (Faldt et al, Planta 216:745-51 (2003); Huang et al, Plant Physiol 153:1293-310 (2010)), afs of Cucumis sativus (Mercke et al, Plant Physiol 135:2012-14 (2004), eafar of Malus x domestica (Green et al, Phytochem 68:176-88 (2007)) and TPS-Far of Picea abies (Martin, supra).
  • An exemplary beta-farnesene synthase enzyme is encoded by TPS1 of Zea mays (Schnee et al, Plant Physiol 130:2049-60 (2002)).
  • pathway intermediates such as 2-oxopent-3-enoate (1N), 2-hydroxypent-3-enoate (1R), 5-hydroxypent-4-enoate (2I), 2,4-pentadienoate (2J), 3-hydroxypent-4-enoate (2L) can be catalyzed by ADP and AMP-forming CoA ligases (6.2.1). These enzymes can also function in the reverse direction to convert the CoA-derivatives to their acid counterparts as shown in Steps 1Q, 3B, 3I, 3J and 3N.
  • ADP-forming acetyl-CoA synthetase (ACD, EC 6.2.1.13) from Archaeoglobus fulgidus , encoded by AF1211, was shown to operate on a variety of linear and branched-chain substrates including isobutyrate, isopentanoate, and fumarate (Musfeldt et al., J Bacteriol. 184:636-644 (2002)).
  • a second reversible ACD in Archaeoglobus fulgidus encoded by AF1983, was also indicated to have a broad substrate range (Musfeldt et al., supra).
  • the enzyme from Haloarcula marismortui annotated as a succinyl-CoA synthetase, accepts propionate, butyrate, and branched-chain acids (isovalerate and isobutyrate) as substrates, and was shown to operate in the forward and reverse directions (Brasen et al., Arch. Microbiol 182:277-287 (2004)).
  • the ACD encoded by PAE3250 from hyperthermophilic crenarchaeon Pyrobaculum aerophilum showed the broadest substrate range of all characterized ACDs, reacting with acetyl-CoA, isobutyryl-CoA (preferred substrate) and phenylacetyl-CoA (Brasen and Schonheit, Arch.
  • Microbiol 182:277-287 (2004) Directed evolution or engineering can be used to modify this enzyme to operate at the physiological temperature of the host organism.
  • the enzymes from A. fulgidus, H. marismortui and P. aerophilum have all been cloned, functionally expressed, and characterized in E. coli (Brasen and Schonheit, Arch. Microbiol 182:277-287 (2004); Musfeldt and Schonheit, J Bacteriol. 184:636-644 (2002)).
  • An additional enzyme is encoded by sucCD in E.
  • acyl CoA ligase from Pseudomonas putida has been indicated to work on several aliphatic substrates including acetic, propionic, butyric, valeric, hexanoic, heptanoic, and octanoic acids and on aromatic compounds such as phenylacetic and phenoxyacetic acids (Fernandez-Valverde et al., Appl. Environ. Microbiol.
  • a related enzyme, malonyl CoA synthetase (6.3.4.9) from Rhizobium leguminosarum could convert several diacids, namely, ethyl-, propyl-, allyl-, isopropyl-, dimethyl-, cyclopropyl-, cyclopropylmethylene-, cyclobutyl-, and benzyl-malonate into their corresponding monothioesters (Pohl et al., J. Am. Chem. Soc. 123:5822-5823 (2001)).
  • acylation of acetate to acetyl-CoA is catalyzed by enzymes with acetyl-CoA synthetase activity.
  • Two enzymes that catalyze this reaction are AMP-forming acetyl-CoA synthetase (EC 6.2.1.1) and ADP-forming acetyl-CoA synthetase (EC 6.2.1.13).
  • AMP-forming acetyl-CoA synthetase (ACS) is the predominant enzyme for activation of acetate to acetyl-CoA.
  • Exemplary ACS enzymes are found in E. coli (Brown et al., J. Gen.
  • Methylmalonyl-CoA synthetase from Rhodopseudomonas palustris converts methylmalonate and malonate to methylmalonyl-CoA and malonyl-CoA, respectively.
  • Structure-based mutagenesis of this enzyme improved CoA synthetase activity with the alternate substrates ethylmalonate and butylmalonate (Crosby et al, AEM, in press (2012)).
  • 4HB-CoA synthetase catalyzes the ATP-dependent conversion of 4-hydroxybutyrate to 4-hydroxybutyryl-CoA.
  • AMP-forming 4-HB-CoA synthetase enzymes are found in organisms that assimilate carbon via the dicarboxylate/hydroxybutyrate cycle or the 3-hydroxypropionate/4-hydroxybutyrate cycle. Enzymes with this activity have been characterized in Thermoproteus neutrophilus and Metallosphaera sedula (Ramos-Vera et al, J Bacteriol 192:5329-40 (2010); Berg et al, Science 318:1782-6 (2007)). Others can be inferred by sequence homology.
  • CoA hydrolysis as described in Steps 1Q, 2J and 2I can be catalyzed by CoA hydrolases or thioesterases in the EC class 3.1.2. Additionally, intermediates such as 3-oxo-4-hydroxypentanoyl-CoA, 3,4-dihydroxypentanoyl-CoA, 4-hydroxypent-2-enoyl-COA, 2,4-pentadienoyl-CoA can be converted into their acid counterparts via thes enzymes as shown in steps 3B, 3I, 3J, 3N respectively. Several CoA hydrolases with broad substrate ranges are suitable enzymes for hydrolyzing these intermediates.
  • the enzyme encoded by acot12 from Rattus norvegicus brain can react with butyryl-CoA, hexanoyl-CoA and malonyl-CoA.
  • the human dicarboxylic acid thioesterase, encoded by acot8 exhibits activity on glutaryl-CoA, adipyl-CoA, suberyl-CoA, sebacyl-CoA, and dodecanedioyl-CoA (Westin et al., J. Biol. Chem. 280:38125-38132 (2005)). The closest E.
  • coli homolog to this enzyme, tesB can also hydrolyze a range of CoA thiolesters (Naggert et al., J Biol Chem 266:11044-11050 (1991)).
  • a similar enzyme has also been characterized in the rat liver (Deana R., Biochem Int 26:767-773 (1992)).
  • Additional enzymes with hydrolase activity in E. coli include ybgC, paaI, yciA, and ybdB (Kuznetsova, et al., FEMS Microbiol Rev, 2005, 29(2):263-279; Song et al., J Biol Chem, 2006, 281(16):11028-38).
  • the enzyme from the mitochondrion of the pea leaf has a broad substrate specificity, with demonstrated activity on acetyl-CoA, propionyl-CoA, butyryl-CoA, palmitoyl-CoA, oleoyl-CoA, succinyl-CoA, and crotonyl-CoA (Zeiher et al., Plant. Physiol. 94:20-27 (1990))
  • the acetyl-CoA hydrolase, ACH1 from S. cerevisiae represents another candidate hydrolase (Buu et al., J. Biol. Chem. 278:17203-17209 (2003)).
  • Yet another candidate hydrolase is the glutaconate CoA-transferase from Acidaminococcus fermentans .
  • This enzyme was transformed by site-directed mutagenesis into an acyl-CoA hydrolase with activity on glutaryl-CoA, acetyl-CoA and 3-butenoyl-CoA (Mack et al., FEBS. Lett. 405:209-212 (1997)).
  • succinyl-CoA:3-ketoacid-CoA transferases and acetoacetyl-CoA:acetyl-CoA transferases may also serve as candidates for this reaction step but would require certain mutations to change their function.
  • Additional hydrolase enzymes include 3-hydroxyisobutyryl-CoA hydrolase which has been described to efficiently catalyze the conversion of 3-hydroxyisobutyryl-CoA to 3-hydroxyisobutyrate during valine degradation (Shimomura et al., J Biol Chem. 269:14248-14253 (1994)). Genes encoding this enzyme include hibch of Rattus norvegicus (Shimomura et al., Methods Enzymol. 324:229-240 (2000)) and Homo sapiens (Shimomura et al., supra). Similar gene candidates can also be identified by sequence homology, including hibch of Saccharomyces cerevisiae and BC_2292 of Bacillus cereus .
  • Methylmalonyl-CoA is converted to methylmalonate by methylmalonyl-CoA hydrolase (EC 3.1.2.7).
  • This enzyme isolated from Rattus norvegicus liver, is also active on malonyl-CoA and propionyl-CoA as alternative substrates (Kovachy et al., J. Biol. Chem., 258: 11415-11421 (1983)). The gene associated with this enzyme is not known.
  • FIGS. 1, 2 and 3 require a CoA transferase to activate carboxylic acids to their corresponding acyl-CoA derivatives and vice versa.
  • the specific transformations are shown in steps 1N, 1R, 1Q, 2I, 2J, 2L, 3B, 3I, 3J.
  • CoA transferase enzymes have been described in the open literature and represent suitable candidates for these steps. These are described below.
  • the gene products of cat1, cat2, and cat3 of Clostridium kluyveri have been shown to exhibit succinyl-CoA, 4-hydroxybutyryl-CoA, and butyryl-CoA transferase activity, respectively (Seedorf et al., Proc. Natl. Acad. Sci U.S.A 105:2128-2133 (2008); Sohling et al., J Bacteriol. 178:871-880 (1996)).
  • a fatty acyl-CoA transferase that utilizes acetyl-CoA as the CoA donor is acetoacetyl-CoA transferase, encoded by the E. coli atoA (alpha subunit) and atoD (beta subunit) genes (Korolev et al., Acta Crystallogr. D. Biol. Crystallogr. 58:2116-2121 (2002); Vanderwinkel et al., 33:902-908 (1968)).
  • This enzyme has a broad substrate range on substrates of chain length C3-C6 (Sramek et al., Arch Biochem Biophys 171:14-26 (1975)) and has been shown to transfer the CoA moiety to acetate from a variety of branched and linear 3-oxo and acyl-CoA substrates, including isobutyrate (Matthies et al., Appl Environ. Microbiol 58:1435-1439 (1992)), valerate (Vanderwinkel et al., Biochem. Biophys. Res. Commun. 33:902-908 (1968)) and butanoate (Vanderwinkel et al., Biochem. Biophys. Res. Commun.
  • Exemplary enzymes for catalyzing the decarboxylation of 2,4-pentadienoate (1H, 2G, 3L), 4-hydroxypent-2-enoate (1T), 5-hydroxypent-2-enoate (2P) and 4-hydroxypent-2-enoate (3E) are sorbic acid decarboxylase, aconitate decarboxylase, 4-oxalocrotonate decarboxylase and cinnamate decarboxylase.
  • Sorbic acid decarboxylase converts sorbic acid to 1,3-pentadiene. Sorbic acid decarboxylation by Aspergillus niger requires three genes: padA1, ohbA1, and sdrA (Plumridge et al. Fung. Genet. Bio, 47:683-692 (2010). PadA1 is annotated as a phenylacrylic acid decarboxylase, ohbA1 is a putative 4-hydroxybenzoic acid decarboxylase, and sdrA is a sorbic acid decarboxylase regulator.
  • decarboxylate sorbic acid including several fungal and yeast species (Kinderlerler and Hatton, Food Addit Contam., 7(5):657-69 (1990); Casas et al., Int J Food Micro., 94(1):93-96 (2004); Pinches and Apps, Int. J. Food Microbiol. 116: 182-185 (2007)).
  • Aspergillus oryzae and Neosartorya fischeri have been shown to decarboxylate sorbic acid and have close homologs to padA1, ohbA1, and sdrA.
  • Aconitate decarboxylase (EC 4.1.1.6) catalyzes the final step in itaconate biosynthesis in a strain of Candida and also in the filamentous fungus Aspergillus terreus (Bonnarme et al. J Bacteriol. 177:3573-3578 (1995); Willke and Vorlop, Appl Microbiol. Biotechnol 56:289-295 (2001)).
  • a cis-aconitate decarboxylase (CAD) (EC 4.1.16) has been purified and characterized from Aspergillus terreus (Dwiarti et al., J. Biosci. Bioeng. 94(1): 29-33 (2002)).
  • decarboxylases catalyze the conversion of cinnamate (phenylacrylate) and substituted cinnamate derivatives to the corresponding styrene derivatives. These enzymes are common in a variety of organisms and specific genes encoding these enzymes that have been cloned and expressed in E.
  • coli are: pad 1 from Saccharomyces cerevisae (Clausen et al., Gene 142:107-112 (1994)), pdc from Lactobacillus plantarum (Barthelmebs et al., 67:1063-1069 (2001); Qi et al., Metab Eng 9:268-276 (2007); Rodriguez et al., J. Agric. Food Chem. 56:3068-3072 (2008)), pofK (pad) from Klebsiella oxytoca (Uchiyama et al., Biosci. Biotechnol. Biochem. 72:116-123 (2008); Hashidoko et al., Biosci. Biotech. Biochem.
  • this class of enzymes have been shown to be stable and do not require either exogenous or internally bound co-factors, thus making these enzymes ideally suitable for biotransformations (Sariaslani, Annu. Rev. Microbiol. 61:51-69 (2007)).
  • 4-Oxalocronate decarboxylase catalyzes the decarboxylation of 4-oxalocrotonate to 2-oxopentanoate.
  • This enzyme has been isolated from numerous organisms and characterized.
  • the decarboxylase typically functions in a complex with vinylpyruvate hydratase. Genes encoding this enzyme include dmpH and dmpE in Pseudomonas sp. (strain 600) (Shingler et al., 174:711-724 (1992)), xylII and xylIII from Pseudomonas putida (Kato et al., Arch.
  • the 4-oxalocrotonate decarboxylase encoded by xylI in Pseudomonas putida functions in a complex with vinylpyruvate hydratase.
  • a recombinant form of this enzyme devoid of the hydratase activity and retaining wild type decarboxylase activity has been characterized (Stanley et al., Biochem. 39:718-26 (2000)).
  • a similar enzyme is found in Ralstonia pickettii (formerly Pseudomonas pickettii ) (Kukor et al., J Bacteriol. 173:4587-94 (1991)).
  • Aspartate decarboxylase (EC 4.1.1.11) decarboxylates aspartate to form beta-alanine. This enzyme participates in pantothenate biosynthesis and is encoded by gene panD in Escherichia coli (Dusch et al., Appl. Environ. Microbiol 65:1530-1539 (1999); Ramjee et al., Biochem. J 323 (Pt 3):661-669 (1997); Merkel et al., FEMS Microbiol Lett.
  • Lysine decarboxylase (EC 4.1.1.18) catalyzes the decarboxylation of lysine to cadaverine.
  • Two isozymes of this enzyme are encoded in the E. coli genome by genes cadA and ldcC.
  • CadA is involved in acid resistance and is subject to positive regulation by the cadC gene product (Lemonnier et al., Microbiology 144 (Pt 3):751-760 (1998)).
  • CadC accepts hydroxylysine and S-aminoethylcysteine as alternate substrates, and 2-aminopimelate and 6-aminocaproate act as competitive inhibitors to this enzyme (Sabo et al., Biochemistry 13:662-670 (1974)).
  • the constitutively expressed lde gene product is less active than CadA (Lemonnier and Lane, Microbiology 144 (Pt 3):751-760 (1998)).
  • a lysine decarboxylase analogous to CadA was recently identified in Vibrio parahaemolyticus (Tanaka et al., J Appl Microbiol 104:1283-1293 (2008)).
  • the lysine decarboxylase from Selenomonas ruminantium encoded by ldc, bears sequence similarity to eukaryotic ornithine decarboxylases, and accepts both L-lysine and L-ornithine as substrates (Takatsuka et al., Biosci.
  • acetolactate decarboxylase (4.1.1.5) which participates in citrate catabolism and branched-chain amino acid biosynthesis, converting the 2-hydroxyacid, 2-acetolactate, to acetoin.
  • Lactococcus lactis the enzyme is composed of six subunits, encoded by gene aldB, and is activated by valine, leucine and isoleucine (Goupil-Feuillerat et al., J. Bacteriol. 182:5399-5408 (2000); Goupil et al., Appl. Environ. Microbiol. 62:2636-2640 (1996)).
  • Tartrate decarboxylase (EC 4.1.1.73) carries out an alpha,beta-hydroxyacid decarboxylation reaction.
  • the enzyme characterized in Pseudomonas sp. group Ve-2, is NAD+ dependent and catalyzes coupled oxidation-reduction reaction that proceeds through an oxaloglycolate intermediate (Furuyoshi et al., J Biochem. 110:520-525 (1991)).
  • a side reaction catalyzed by this enzyme is the NAD+ dependent oxidation of tartrate (1% of activity).
  • a gene has not been associated with this enzyme activity to date.
  • keto-acids such as 4-hydroxy 2-oxovalerate, 2-oxopent-3-enoate as shown in Steps 1C and 1I
  • pyruvate decarboxylase EC 4.1.1.1
  • benzoylformate decarboxylase EC 4.1.1.7
  • alpha-ketoglutarate decarboxylase EC 4.1.1.7
  • alpha-ketoglutarate decarboxylase EC branched-chain alpha-ketoacid decarboxylase.
  • PDC Pyruvate decarboxylase
  • keto-acid decarboxylase is a key enzyme in alcoholic fermentation, catalyzing the decarboxylation of pyruvate to acetaldehyde.
  • the enzyme from Saccharomyces cerevisiae has a broad substrate range for aliphatic 2-keto acids including 2-ketobutyrate, 2-ketovalerate, 3-hydroxypyruvate and 2-phenylpyruvate (22).
  • This enzyme has been extensively studied, engineered for altered activity, and functionally expressed in E. coli (Killenberg-Jabs et al., Eur. J. Biochem. 268:1698-1704 (2001); Li et al., Biochemistry. 38:10004-10012 (1999); ter Schure et al., Appl. Environ. Microbiol. 64:1303-1307 (1998)).
  • the PDC from Zymomonas mobilus also has a broad substrate range and has been a subject of directed engineering studies to alter the affinity for different substrates (Siegert et al., Protein Eng Des Sel 18:345-357 (2005)).
  • the crystal structure of this enzyme is available (Killenberg-Jabs et al., Eur. J. Biochem. 268:1698-1704 (2001)).
  • Other well-characterized PDC candidates include the enzymes from Acetobacter pasteurians (Chandra et al., 176:443-451 (2001)) and Kluyveromyces lactis (Krieger et al., 269:3256-3263 (2002)).
  • benzoylformate decarboxylase (EC 4.1.1.7) has a broad substrate range and has been the target of enzyme engineering studies.
  • the enzyme from Pseudomonas putida has been extensively studied and crystal structures of this enzyme are available (Polovnikova et al., 42:1820-1830 (2003); Hasson et al., 37:9918-9930 (1998)).
  • Site-directed mutagenesis of two residues in the active site of the Pseudomonas putida enzyme altered the affinity (Km) of naturally and non-naturally occurring substrates (Siegert et al., Protein Eng Des Sel 18:345-357 (2005)).
  • This enzyme has been further modified by directed engineering (Lingen et al., Chembiochem. 4:721-726 (2003); Lingen et al., Protein Eng 15:585-593 (2002)).
  • the enzyme from Pseudomonas aeruginosa encoded by mdlC, has also been characterized experimentally (Barrowman et al., 34:57-60 (1986)). Additional gene candidates from Pseudomonas stutzeri, Pseudomonas fluorescens and other organisms can be inferred by sequence homology or identified using a growth selection system developed in Pseudomonas putida (Henning et al., Appl. Environ. Microbiol. 72:7510-7517 (2006)).
  • a third enzyme capable of decarboxylating 2-oxoacids is alpha-ketoglutarate decarboxylase (KGD, EC 4.1.1.71).
  • the substrate range of this class of enzymes has not been studied to date.
  • An exemplarly KDC is encoded by kgd in Mycobacterium tuberculosis (Tian et al., PNAS 102:10670-10675 (2005)).
  • KDC enzyme activity has also been detected in several species of rhizobia including Bradyrhizobium japonicum and Mesorhizobium loti (Green et al., J Bacteriol 182:2838-2844 (2000)).
  • KDC-encoding gene(s) have not been isolated in these organisms, the genome sequences are available and several genes in each genome are annotated as putative KDCs.
  • a KDC from Euglena gracilis has also been characterized but the gene associated with this activity has not been identified to date (Shigeoka et al., Arch. Biochem. Biophys. 288:22-28 (1991)).
  • the first twenty amino acids starting from the N-terminus were sequenced MTYKAPVKDVKFLLDKVFKV (Shigeoka and Nakano, Arch. Biochem. Biophys. 288:22-28 (1991)).
  • the gene could be identified by testing candidate genes containing this N-terminal sequence for KDC activity.
  • BCKA branched chain alpha-ketoacid decarboxylase
  • Lactococcus lactis has been characterized on a variety of branched and linear substrates including 2-oxobutanoate, 2-oxohexanoate, 2-oxopentanoate, 3-methyl-2-oxobutanoate, 4-methyl-2-oxobutanoate and isocaproate (Smit et al., Appl Environ Microbiol 71:303-311 (2005)).
  • the enzyme has been structurally characterized (Berg et al., Science. 318:1782-1786 (2007)).
  • IPDA Indolepyruvate decarboxylase
  • Recombinant branched chain alpha-keto acid decarboxylase enzymes derived from the E1 subunits of the mitochondrial branched-chain keto acid dehydrogenase complex from Homo sapiens and Bos taurus have been cloned and functionally expressed in E. coli (Davie et al., J. Biol. Chem. 267:16601-16606 (1992); Wynn et al., J. Biol. Chem. 267:12400-12403 (1992); Wynn et al., J. Biol. Chem. 267:1881-1887 (1992)).
  • An additional example is 2-hydroxyglutaryl-CoA dehydratase encoded by hgdABC from Acidaminococcus fermentans (Mueller and Buckel, Eur. J. Biochem. 230:698-704 (1995); Schweiger et al., Eur. J. Biochem. 169:441-448 (1987)).
  • Purification of the dehydratase from A. fermentans yielded two components, A and D.
  • Component A HgdC
  • Component D is the actual dehydratase and is encoded by HgdAB.
  • Component A the activator, from A. fermentans is active with the actual dehydratse (component D) from C. symbiosum and is reported to have a specific activity of 60 per second, as compared to 10 per second with the component D from A. fermentans .
  • component D the actual dehydratse
  • Yet another example is the 2-hydroxyisocaproyl-CoA dehydratase from Clostridium difficile catalyzed by hadBC and activated by hadI (Darley et al., FEBS J. 272:550-61 (2005)). The sequence of the complete C.
  • propionicium lactoyl-CoA dehydratase is not yet listed in publicly available databases.
  • sequence of the beta-subunit corresponds to the GenBank accession number AJ276553 (Selmer et al, Eur J Biochem, 269:372-80 (2002)).
  • the dehydratase from Clostridium sporogens that dehydrates phenyllactyl-CoA to cinnamoyl-CoA is also a potential candidate for this step.
  • This enzyme is composed of three subunits, one of which is a CoA transferase.
  • the first step comprises of a CoA transfer from cinnamoyl-CoA to phenyllactate leading to the formation of phenyllactyl-CoA and cinnamate.
  • the product cinnamate is released.
  • the dehydratase then converts phenyllactyl-CoA into cinnamoyl-CoA.
  • FldA is the CoA transferase and FldBC are related to the alpha and beta subunits of the dehydratase, component D, from A. fermentans .
  • Another dehydratase that can potentially conduct such a biotransformation is the enoyl-CoA hydratase (4.2.1.17) of Pseudomonas putida , encoded by ech that catalyzes the conversion of 3-hydroxybutyryl-CoA to crotonyl-CoA (Roberts et al., Arch. Microbiol 117:99-108 (1978)).
  • This transformation is also catalyzed by the crt gene product of Clostridium acetobutylicum , the crt1 gene product of C.
  • the E. coli gene products of fadA and fadB encode a multienzyme complex involved in fatty acid oxidation that exhibits enoyl-CoA hydratase activity (Yang et al., Biochemistry 30:6788-6795 (1991); Yang, J Bacteriol. 173:7405-7406 (1991); Nakahigashi et al., Nucleic Acids Res. 18:4937 (1990)).
  • Knocking out a negative regulator encoded by fadR can be utilized to activate the fadB gene product (Sato et al., J Biosci. Bioeng 103:38-44 (2007)).
  • the fadI and fadJ genes encode similar functions and are naturally expressed under anaerobic conditions (Campbell et al., Mol. Microbiol 47:793-805 (2003)).
  • crotyl alcohol (1M, 4H), methyl vinyl carbinol (1L, 2Q, 3F, 4G), 3-hydroxybutyraldehyde (1D), 4-hydroxy-2-oxovalerate (1E, 2C), 2-hydroxy pent3-enoate (1G), 4-hydroxypent-2-enoate (1V, 3K), 5-hydroxypent-2-enoate (2F), 3-hydroxypent-4-enoate (2R) and 3,4-dihydroxypentanoate (3D) can be catalyzed exemplary dehydratases including oleate hydratase, acyclic 1,2-hydratase, linalool dehydratase, dimethylmaleate hydratase, (S)-2-methylmalate dehydratase, fumarate hydratase, glycerol dehydratase and enoyl-CoA hydratase enzymes. Enzyme candidates are described below.
  • crotyl alcohol, 3-buten-2-ol and 3-buten-1-ol produced by culturing the non-naturally occurring microbial organisms described herein can be converted to butadiene by chemical dehydration in the presence of a chemical catalyst.
  • a chemical catalyst for example see international patent application publication WO2012106516A1.
  • Oleate hydratases catalyze the reversible hydration of non-activated alkenes to their corresponding alcohols.
  • Oleate hydratase enzymes disclosed in WO2011/076691 and WO 2008/119735 are incorporated by reference herein.
  • Oleate hydratases from Elizabethkingia meningoseptica and Streptococcus pyogenes are encoded by ohyA and HMPREF0841_1446.
  • Acyclic 1,2-hydratase enzymes (eg. EC 4.2.1.131) catalyze the dehydration of linear secondary alcohols, and are thus suitable candidates for the dehydration of MVC to butadiene.
  • Exemplary 1,2-hydratase enzymes include carotenoid 1,2-hydratase, encoded by crtC of Rubrivivax gelatinosus (Steiger et al, Arch Biochem Biophys 414:51-8 (2003)), and lycopene 1,2-hydratase, encoded by cruF of Synechococcus sp. PCC 7002 and Gemmatimonas aurantiaca (Graham and Bryant, J Bacteriol 191: 2392-300 (2009); Takaichi et al, Microbiol 156: 756-63 (2010)).
  • Linalool dehydratase/isomerase of Castellaniella defragrans catalyzes the dehydration of linalool to myrcene (Brodkorb et al, J Biol Chem 285:30436-42 (2010)). Enzyme accession numbers and homologs are listed in the table below.
  • Dimethylmaleate hydratases catalyze the dehydration of (2R,3S)-2,3-dimethylmalate into dimethylmaleate (EC 4.2.1.85).
  • Dimethylmaleate hydratases from Eubacterium barkeri are encoded by dmdA and dmdB (Alhapel et al., Proc Natl Acad Sci 103:12341-6 (2006)).
  • (S)-2-methylmalate dehydratases catalyze the reversible hydration of mesaconate to citramalate (EC 4.2.1.34).
  • An exemplary (S)-2-methylmalate dehydratase is encoded by LeuC and LeuD of Methanococcus jannaschii and has been shown to catalyze the second step in the leucine biosynthesis pathway (Lee et al, Biochem Biophys Res Commun 419(2):160-4 (2012)). Fumarate hydratases catalyze the interconversion of fumarate to malate (EC 4.2.1.2). Two classes of fumarate hydratases exist, where classification is dependent upon the arrangement of subunits and metal requirements.
  • Exemplary class I and class II fumarate dehydratases are encoded by fumA and fumC of Escherichia coli (Tseng et al, J Bacteriol 183(2):461-7 (2001)), and fumC of Corynebacterium glutamicum (Genda et al, Biosci Biotechnol Biochem 70(5):1102-9 (2006)).
  • Glycerol dehydratases (EC 4.2.1.30) catalyze the conversion of glycerol to 3-hydroxy-propionaldehyde and water.
  • Exemplary glycerol dehydratases are encoded by dhaB of Klebsiella pneumoniae (Wang et al, Biotechnol J.
  • Enoyl-CoA hydratases catalyze the hydration of the double bond between the second and third carbons on acyl-CoA (EC 4.2.1.17). Enoyl-CoA hydratases are involved in the breakdown of fatty acids. Exemplary enoyl-CoA hydratases are encoded by phaJ1 of Pseudomonas putida (Vo et al, J. Biosci. Bioeng. 106 (1), 95-98 (2008)), and paaF of Escherichia coli (Teufel et al, Proc Natl Acad Sci USA. 107(32):14390-5 (2010)).
  • FIG. 1 Steps G, M
  • FIG. 4 Steps F, G and H
  • Bifunctional enzymes with dehydratase and isomerase activities are suitable for dehydrating and rearranging alcohols to alkenes as shown in FIGS. 1-4 .
  • This type of enzyme is required to convert 2-hydroxypent-3-enoate to 2,4-pentadienoate (Step G of FIG. 1 ) and crotyl alcohol to butadiene ( FIG. 1M and FIG. 4H ).
  • transformation 1 G can be catalyzed by the isomerization of 2-hydroxypent-3-enoate to 4-hydroxypent-2-enoate, followed by a dehydration of 4-hydroxypent-2-enoate to 2,4-pentadienoate.
  • An exemplary bifunctional enzyme with isomerase and dehydratase activities is the linalool dehydratase/isomerase of Castellaniella defragrans .
  • This enzyme catalyzes the isomerization of geraniol to linalool and the dehydration of linalool to myrcene, reactants similar in structure to CrotOH, MVC, 2-hydroxypent-3-enoate, butadiene and 2,4-pentadienoate and is also active on crotyl alcohol (Brodkorb et al, J Biol Chem 285:30436-42 (2010)). Enzyme accession numbers and homologs are listed in the table below.
  • a fusion protein or protein conjugate can be generated using well know methods in the art to generate a bi-functional (dual-functional) enzyme having both the isomerase and dehydratase activities.
  • the fusion protein or protein conjugate can include at least the active domains of the enzymes (or respective genes) of the isomerase and dehydratase reactions.
  • enzymatic conversion can be catalyzed by a CrotOH or 2-hydroxypent-3-enoate isomerase (classified as EC 5.3.3 and EC 5.4.4).
  • Linalool dehydratase/isomerase Genbank ID number 403399445
  • E. coli variants were transformed with the expression plasmid and selected and maintained using antibiotic selection with carbenicillin.
  • Vinylisomerase catalyzes the conversion of Crotyl alcohol to MVC (1K, 4F), 2-hydroxypent-3-enoate to 4-hydroxypent-2-enoate (1U), 3-hydroxypent-4-enoyl-CoA to 5-hydroxypent-2-enoyl-CoA (2M) and 2-hydroxypent-4-enoate to 5-hydroxypent-2-enoate (2E).
  • the conversion of 3-buten-1-ol to crotyl alcohol can be carried out by vinyl-isomerases (see FIG. 2 Step S).
  • the conversion of 3-buten-2-ol to crotyl alcohol can be carried out by vinyl-isomerases (see FIG. 1 Step W and FIG. 3 Step O).
  • enzymes include isopentenyl-diphosphate ⁇ -isomerase, vinylacetyl-CoA ⁇ -isomerase and methylitaconate ⁇ -isomerase. Enzymes candidates are described below. Also useful is the vinylisomerase activity of linalool dehydratase.
  • Isopentenyl diphophaste isomerases catalyze the interconversion of isopentenyl diphosphate and dimethylallyl diphosphate, but can also catalyze the interconversion of CrotOH to MC (EC 5.3.3.2).
  • Exemplary isopentenyl diphophaste isomerases are encoded by IDI-2 of Thermus thermophilus (Sharma et al, Biochemistry 49(29): 6228-6233 (2010)), idi of Xanthophyllomyces dendrorhous and idi of Haematococcus pluvialis (Kajiwara et al, Biochem J 324(Pt 2): 421-426 (1997)).
  • Crystal structures have been determined for the isopentenyl diphophaste isomerases from Escherichia coli (Durbecq et al, EMBO J 20(7): 1530-1537 (2001)) and from Methanocaldococcus jannaschii (Hoshino et al, Acta Crystallogr Sect F Struct Biol Cryst Commun 67(Pt 1): 101-103 (2011)). Enzyme accession numbers and homologs are listed in the table below.
  • Vinylacetyl-CoA ⁇ -isomerases catalyze the conversion of vinylacetyl-CoA to crotonyl-CoA (EC 5.3.3.3).
  • Exemplary vinylacetyl-CoA ⁇ -isomerases are encoded by AbfD of Clostridium kluyveri (Scherf et al, Arch Microbiol 161(3):239-45 (1994)), abfD of Clostridium aminobutyricum (Scherf et al, Eur J Biochem 215(2):421-9 (1993)), and Msed_1321 of Metallosphaera sedula (Auemik et al, Appl Environ Microbiol 74(3):682-92 (2008)).
  • Methylitaconate ⁇ -isomerases catalyze the isomerization of itaconate (methylenesuccinate) to citraconate (methylmaleate) (EC 5.3.3.6).
  • An exemplary methylitaconate ⁇ -isomerase is encoded by mii from Eubacterium barkeri (Alhapel et al, Proc Natl Acad Sci USA 103(33):12341-6 (2006)) and the crystal structure of this 3-methylitaconate-delta-isomerase has been determined (Velarde et al, J Mol Biol 391(3):609-20 (2009)). Enzyme accession numbers and homologs are listed in the table below.
  • Alcohol mutases that catalyze the conversion of 2-hydroxypent-4-enoate to 5-hydroxypent-2-enoate (2E) and 2-hydroxypent-4-enoate to 3-hydroxypent-4-enoate (2H) are found in the EC 5.4.4 enzyme class, which include isomerases that transfer hydroxyl groups.
  • Exemplary isomerase enzymes suitable for the conversion of 2-hydroxypent-4-enoate to 5-hydroxypent-2-enoate include isochorismate synthase (EC 5.4.4.2) and geraniol isosmerase (EC 5.4.4.4). Isochorismate synthase catalyzes the isomerization of chorismate to isochorismate and encodes for essential components of the respiratory chain.
  • Exemplary isochorismate synthases are encoded by menF and dhbC of Bacillus subtilis (Rowland et al, J Bacteriol. 178(3):854-61 (1996)) and by menF of Escherichia coli (Daruwala et al, J Bacteriol. 179(10):3133-8 (1997)).
  • Geraniol isomerase catalyzes the isomerization of (3S)-linalool to geraniol.
  • Exemplary geraniol isomerase is encoded by Ldi of Castellaniella defragrans (Brodkorb et al, J Biol Chem 285:30436-42 (2010)). Enzyme accession numbers and homologs are listed in the table below.
  • Alkenol synthase catalyzes the conversion of 2-butenyl-4-phosphate and 2-butenyl-4-diphosphate to 3-buten-2-ol or MVC (4D and 4E respectively).
  • the enzymes described below naturally possess such activity or can be engineered to exhibit this activity.
  • Particularly useful enzymes include methylbutenol (MBO) synthase, linalool synthase and nerolidol synthase. Enzyme candidates are described below and found within EC 4.2.3.
  • Methylbutenol synthase naturally catalyzes the conversion of dimethylallyl diphosphate to methylbutenol, but can also catalyze the synthesis of 3-buten-2-ol or MVC from 2-butenyl-4-phosphate and 2-butenyl-4-diphosphate.
  • An exemplary methylbutenol synthase is encoded by Tps-MBO1 of Pinus sabiniana (Gray et al, J Biol Chem. 286(23):20582-90 (2011)). Linalool synthases catalyze the conversion of geranyl diphosphate to linalool.
  • Exemplary R- (EC 4.2.3.26) and S-linalool synthases (EC 4.2.3.25) are encoded by AY083653 of Mentha citrata (Crowell et al, Arch Biochem Biophys., 405(1):112-21 (2002)) and by Lis of Clarkia breweri (Dudareva et al, Plant Cell. 8(7): 1137-1148 (1996)), respectively.
  • (3S, 6E)-nerolidol synthase (EC 4.2.3.48) catalyze the conversion of farnesyl diphosphate to nerolidol.
  • BDS (monophosphate) catalyzes the conversion of 2-butenyl-4-phosphate to 1,3-butadiene (Step 4J).
  • BDS enzymes described above for Step C in the EC 4.2.3 enzyme class may possess such activity or can be engineered to exhibit this activity.
  • Example 2 Decarboxylation of 2,4-Pentadienoate to Butadiene by a Phenylacrylate Decarboxylase
  • PadA1 (GI number: 1165293) and OhbA1 (GI number: 188496963) encoding phenylacrylate decarboxylase from S. cerevisiae were codon optimized by DNA 2.0 and were cloned by DNA 2.0 into the following vectors suitable for expression in E. coli , pD424-NH and pD441-NH respectively (DNA 2.0 Inc.).
  • the genes were tested for decarboxylation of 2,4-pentadienoate and the enzymatic reactions were carried out under the following conditions:
  • butadiene was measured when 2,4-PD was added as a substrate.
  • Example 3 Demonstration of Acetyl-CoA Reductase (1A Ad 2A), 4-Hydroxy 2-Oxovalerate Aldolase (1B and 2B), 4-Hydroxy 2-Oxovalerate Decarboxylase (1C)
  • acetyl-CoA reductase (bphJ from Burkholderia xenovorans LB400, GI no: 520923), 4-hydroxy 2-oxovalerate aldolase (bphI from Burkholderia xenovorans LB400, GI no: 520924), 4-hydroxy 2-oxovalerate decarboxylase (kdc from Mycobacterium tuberculosis BcG H37Rv, GI no: 614088617), and alcohol dehydrogenase (yjgB from Chronobacter sakazakii , GI no: 387852894) were cloned into a plasmid suitable for expression in E.
  • coli plasmid pZA23S (kanamycin resistance marker, p15A origin of replication) obtained from R. Lutz (Expressys, Germany) and are based on the pZ Expression System (Lutz, R. & Bujard, H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-12 regulatory elements. Nucleic Acids Res. 25, 1203-1210 (1997)).
  • E. coli (MG1655 variants) cells were transformed with the expression plasmid and selected and maintained using antibiotic selection with Kanamycin. Cells were grown for 72 hours in LB media with kanamycin and IPTG at 37° C. then harvested by centrifugation. The formation of a 4-carbon diol derived from 3-hydroxybutyraldehyde using glucose as the carbon substrate was measured (data not shown) while the empty vector control did not make any 4-carbon diol.
  • Isoprene synthase E.C. 4.2.3.27, Genbank ID number 63108310, was cloned from Populus alba into a plasmid suitable for expression in E. coli , plasmid pZS*13S (Expressys, Germany).
  • E. coli (MG1655 variants) were transformed with the expression plasmid and selected and maintained using antibiotic selection with carbenicillin.
  • Cells were grown in Terrific Broth with carbenicillin to an OD of 0.8 and then gene expression induced by IPTG addition then harvested by centrifugation. Lysis was performed using microfluidization at 0° C. Streptactin-tagged isoprene synthase was isolated from the cell lysate using Streptactin-Sepharose purification.
  • Purified enzyme was tested for its ability to convert its native substrate, dimethylallyl diphosphate, into isoprene, and for its ability to convert crotyl diphosphate into 1, 3-butadiene, by incubating purified enzyme with each substrate in sealed screw-cap vials for a period of time before analysis of product in headspace of vial by GC-MS. Fidelity of purified enzyme was confirmed by detection of isoprene. Activity on crotyl diphosphate was confirmed by detection of butadiene. In the absence of enzyme, no butadiene was formed (data not shown).
  • Isopentenyl phosphate kinase E.C. 2.7.4.26, Genbank ID number 2621082, was cloned from Methanobacterium thermoautotrophicum gi12621082 into a plasmid suitable for expression in E. coli , plasmid pZS*13S obtained from R. Lutz (Expressys, Germany) and are based on the pZ Expression System (Lutz, R. & Bujard, H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 25, 1203-1210 (1997)).
  • E. coli (MG1655 variants) were transformed with the expression plasmid and selected and maintained using antibiotic selection with carbenicillin.
  • Cells were grown in LB media with carbenicillin and IPTG at 37° C. then harvested by centrifugation. Lysis was performed using a chemical lysis procedure, and lysate the cooled to 4° C. Streptactin-tagged isopentenyl phosphate kinase was isolated from the cell lysate using Streptactin-Sepharose purification.
  • Isopentenyl-diphosphate DELTA-isomerase (IPP isomerase), E.C. 5.3.3.2, Genbank ID number 3790386, was cloned from Xanthophyllomyces dendrorhous into a plasmid suitable for expression in E. coli , plasmid pZS*13S obtained from R. Lutz (Expressys, Germany) and are based on the pZ Expression System (Lutz, R. & Bujard, H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 25, 1203-1210 (1997)).
  • E. coli (MG1655 variants) were transformed with the expression plasmid and selected and maintained using antibiotic selection with carbenicillin.
  • Cells were grown in LB media with carbenicillin and IPTG at 37° C. then harvested by centrifugation.
  • Cell lysates containing the IPP isomerase from Xanthophyllomyces dendrorhous were generated by sonicating cells resuspended in 100 mM Tris, 50 mM KCl, 5 mM MgSO4, pH 7.2, and 5 mM DTT. 50 mM CrotOH was added to resulting cell lysate at 2.3 mg/ml total protein, and the reaction mixture incubated at 25° C. for 16 hours.
  • MVC or 3-buten-2-ol was measured using headspace analysis on an Agilent 7890A GC equipped with a CTC-PAL autosampler and a MSD (5975C). Samples were diluted 2-fold in 100% methanol to a total volume of 0.100 mL, and transferred into glass inserts in 1.5 mL GC vials. Samples were injected by a Combi PAL CTC autosampler operated in direct injection mode with an injection volume of 1.0 ⁇ L (split ratio 20:1) and 200° C. inlet temperature. Helium was used as a carrier gas, and the flow rate maintained at 1.28 mL/min. The oven temperature is initially held at 100° C. for 1 minute, then ramping 100° C./min to 230° C., for 3 minutes. The MVC or 3-buten-2-ol concentration in samples were calculated from calibration curves generated from diluted MVC standards analyzed under the same GCMS method.
  • Alcohol dehydrogenase Genbank ID number 407959257 was cloned from Synechocystis sp. PCC 6803 into a plasmid suitable for expression in E. coli , plasmid pZS*13S obtained from R. Lutz (Expressys, Germany) and are based on the pZ Expression System (Lutz, R. & Bujard, H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 25, 1203-1210 (1997)).
  • E. coli (MG1655 variants) were transformed with the expression plasmid and selected and maintained using antibiotic selection with carbenicillin.
  • Cells were grown in LB media with carbenicillin and IPTG at 37° C. then harvested by centrifugation. Lysis was performed using a chemical lysis procedure, and lysate was then cooled to 4° C.
  • Streptactin-tagged alcohol dehydrogenase was isolated from the cell lysate using Streptactin-Sepharose purification.
  • Activity measurements assessing the NADPH-dependent reduction of crotonaldehyde to crotonol were performed.
  • the alcohol dehydrogenase from Synechocystis sp. PCC 6803 was found to have a KM value of 0.16 mM and a Kcat of at least 36 s-1. In the absence of enzyme, no reduction of crotonaldehyde to crotonol was observed.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides a non-naturally occurring microbial organism having a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol, pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a pathway. The invention additionally provides a method for producing butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol. The method can include culturing a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a pathway enzyme in a sufficient amount, and under conditions and for a sufficient period of time to produce butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol.

Description

    PRIORITY CLAIM
  • This application claims the benefit of U.S. Provisional Patent Application Ser. No. 62/020,901 filed Jul. 3, 2014, and U.S. Provisional Patent Application Ser. No. 62/082,747 filed Nov. 21, 2014, both applications entitled MICROORGANISMS FOR PRODUCING 4C-5C COMPOUNDS WITH UNSATURATION AND METHODS RELATED THERETO, the disclosures of which are incorporated herein by reference.
    • FIELD OF THE INVENTION
  • The present invention relates generally to metabolic and biosynthetic processes and microbial organisms capable of producing organic compounds, and more specifically to non-naturally occurring microbial organisms having an organic compound pathway, such as butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and 2,4-pentadienoate.
    • BACKGROUND OF THE INVENTION
  • Over 25 billion pounds of butadiene (1,3-butadiene, “BD”) are produced annually and is applied in the manufacture of polymers such as synthetic rubbers and ABS resins, and chemicals such as hexamethylenediamine and 1,4-butanediol. For example, butadiene can be reacted with numerous other chemicals, such as other alkenes, e.g. styrene, to manufacture numerous copolymers, e.g. acrylonitrile 1,3-butadiene styrene (ABS), styrene-1,3-butadiene (SBR) rubber, styrene-1,3-butadiene latex. These materials are used in rubber, plastic, insulation, fiberglass, pipes, automobile and boat parts, food containers, and carpet backing. Butadiene is typically produced as a by-product of the steam cracking process for conversion of petroleum feedstocks such as naphtha, liquefied petroleum gas, ethane or natural gas to ethylene and other olefins.
  • The ability to manufacture butadiene from alternative and/or renewable feedstocks would represent a major advance in the quest for more sustainable chemical production processes.
  • One possible way to produce butadiene renewably involves fermentation of sugars or other feedstocks to produce diols, such as 1,4-butanediol or 1,3-butanediol, which are separated, purified, and then dehydrated to butadiene in a second step involving metal-based catalysis.
  • Direct fermentative production of butadiene from renewable feedstocks would obviate the need for dehydration steps and butadiene gas (bp −4.4° C.) would be continuously emitted from the fermenter and readily condensed and collected. Developing a fermentative production process would eliminate the need for fossil-based butadiene and would allow substantial savings in cost, energy, and harmful waste and emissions relative to petrochemically-derived butadiene.
  • Crotyl alcohol (“CrotOH”), also referred to as 2-buten-1-ol, is a valuable chemical intermediate. It serves as a precursor to crotyl halides, esters, and ethers, which in turn are chemical intermediates in the production of monomers, fine chemicals, agricultural chemicals, and pharmaceuticals. Exemplary fine chemical products include sorbic acid, trimethylhydroquinone, crotonic acid and 3-methoxybutanol. CrotOH is also a precursor to 1,3-butadiene. CrotOH is currently produced exclusively from petroleum feedstocks. For example Japanese Patent 47-013009 and U.S. Pat. Nos. 3,090,815, 3,090,816, and 3,542,883 describe a method of producing CrotOH by isomerization of 1,2-epoxybutane. The ability to manufacture CrotOH from alternative and/or renewable feedstocks would represent a major advance in the quest for more sustainable chemical production processes.
  • 3-Buten-2-ol (also referenced to as methyl vinyl carbinol (“MVC”)) is an intermediate that can be used to produce butadiene. There are significant advantages to use of MVC over 1,3-BDO because there are fewer separation steps and only one dehydration step. MVC can also be used as a solvent, a monomer for polymer production, or a precursor to fine chemicals. Accordingly, the ability to manufacture MVC from alternative and/or renewable feedstock would again present a significant advantage for sustainable chemical production processes.
  • 2,4-Pentadienoate is a useful substituted butadiene derivative in its own right and a valuable intermediate en route to other substituted 1,3-butadiene derivatives, including, for example, 1-carbamoyl-1,3-butadienes which are accessible via Curtius rearrangement. The resultant N-protected-1,3-butadiene derivatives can be used in Diels alder reactions for the preparation of substituted anilines. 2,4-Pentadienoate can be used in the preparation of various polymers and co-polymers.
  • Thus, there exists a need for alternative methods for effectively producing commercial quantities of compounds such as butadiene, CrotOH, MVC or 2,4-pentadienoate. The present invention satisfies this need and provides related advantages as well.
    • SUMMARY OF THE INVENTION
  • In embodiments the invention provides a non-naturally occurring microbial organism having a pathway to butadiene, crotyl alcohol, 2,4-pentadienoate, or 3-buten-2-ol, said microbial organism comprising at least one exogenous nucleic acid encoding a pathway enzyme, wherein the enzyme is selected from the group consisting of (1E) 4-hydroxy 2-oxovalerate decarboxylase, (1 D) 3-hydroxybutyraldehyde dehydratase, (1N) 2-oxopent-3-enoyl-CoA synthetase or transferase, (1O) 2-oxopent-3-enoyl-CoA reductase, (1P) 2-hydroxypent-3-enoyl-CoA dehydratase/vinylisomerase, (1R) 2-hydroxypent-3-enoyl-CoA synthetase or transferase, (1F) 2-oxopent-3-enoate reductase, (1U) 2-hydroxypent-3-enoate vinylisomerase, and (1K) crotyl alcohol vinylisomerase. One or more additional pathway enzymes may be included in the microbial organism. The invention also provides methods for the production of butadiene, crotyl alcohol, 2,4-pentadienoate, or 3-buten-2-ol, comprising culturing the non-naturally occurring microorganism.
  • In other embodiments the invention provides a non-naturally occurring microbial organism having a pathway to butadiene, crotyl alcohol, 2,4-pentadienoate, or 3-buten-1-ol, said microbial organism comprising at least one exogenous nucleic acid encoding a pathway enzyme, the enzyme selected from the group consisting of (2E) 2-hydroxypent-4-enoate vinylisomerase, (2H) 2-hydroxypent-4-enoate mutase, and (2M) 3-hydroxypent-4-enoyl-CoA vinylisomerase. One or more additional pathway enzymes may be included in the microbial organism. The invention also provides methods for the production of butadiene, crotyl alcohol, 2,4-pentadienoate, or 3-buten-1-ol, comprising culturing the non-naturally occurring microorganism.
  • In other embodiments the invention provides a non-naturally occurring microbial organism having a pathway to butadiene, 2,4-pentadienoate, or 3-buten-2-ol, said microbial organism comprising at least one exogenous nucleic acid encoding a pathway enzyme, the enzyme selected from the group consisting of (3D) 3,4-dihydroxypentanoate dehydratase, (3E) 4-hydroxypent-2-enoate decarboxylase (3H) 3,4-dihydroxypentanoyl-CoA dehydratase, and (3J) 4-hydroxypent-2-enoyl-CoA transferase. The invention also provides methods for the production of butadiene, crotyl alcohol, 2,4-pentadienoate, or 3-buten-2-ol, comprising culturing the non-naturally occurring microorganism.
  • In other embodiments the invention provides a non-naturally occurring microbial organism having a pathway to convert crotyl alcohol to butadiene or 3-buten-2-ol, said microbial organism comprising at least one exogenous nucleic acid encoding a pathway enzyme, wherein the enzyme is selected from the group consisting of (4D) 3-buten-2-ol synthase, (4E) 3-buten-2-ol synthase, or (4F) crotyl alcohol isomerase.
    • BRIEF DESCRIPTION OF THE DRAWINGS
  • FIG. 1 shows pathways to butadiene from pyruvate and acetyl-CoA. Enzymes are A. acetyl-CoA reductase, B. 4-hydroxy 2-oxovalerate aldolase, C. 4-hydroxy 2-oxovalerate decarboxylase, D. 3-hydroxybutyraldehyde dehydratase, E. 4-hydroxy-2-oxovalerate 3-dehydratase, F. 2-oxopent-3-enoate reductase, G. 2-hydroxypent-3-enoate dehydratase/vinylisomerase, H. 2,4-pentadienoate decarboxylase, I. 2-oxopent-3-enoate decarboxylase, J. crotyl aldehyde reductase, K. crotyl alcohol vinylisomerase, L. 3-buten-2-ol dehydratase, M. crotyl alcohol dehydratase/vinylisomerase, N. 2-oxopent-3-enoyl-CoA synthetase or transferase, O. 2-oxopent-3-enoyl-CoA reductase, P. 2-hydroxypent-3-enoyl-CoA dehydratase/vinylisomerase, Q. 2,4-pentadienoyl-CoA synthetase, transferase or hydrolase, R. 2-hydroxypent-3-enoyl-CoA synthetase or transferase, S. 2-hydroxypent-3-enoate decarboxylase, T. 4-hydroxypent-2-enoate decarboxylase, U. 2-hydroxypent-3-enoate vinylisomerase, V. 4-hydroxypent-2-enoate dehydratase, W. vinylisomerase.
  • FIG. 2 shows pathways to butadiene from pyruvate and acetyl-CoA. Acetyl-CoA reductase, B. 4-hydroxy 2-oxovalerate aldolase, C. 4-hydroxy 2-oxovalerate dehydratase, D. 2-oxopent-4-enoate reductase, E. 2-hydroxypent-4-enoate vinylisomerase, F. 5-hydroxypent-2-enoate dehydratase, G. 2,4-pentadienoate decarboxylase, H. 2-hydroxypent-4-enoate mutase, I. 5-hydroxypent-2-enoyl-CoA synthetase, transferase or hydrolase, J. 2,4-pentadienoyl-CoA synthetase, transferase or hydrolase, K. 3-hydroxypent-4-enoate decarboxylase, L. 3-hydroxypent-4-enoyl-CoA synthetase or transferase, M. 3-hydroxypent-4-enoyl-CoA vinylisomerase, N. 2,4-pentadienoyl-CoA synthetase, hydrolase or transferase, O. 3-hydroxypent-4-enoyl-CoA dehydratase, P. 5-hydroxypent-2-enoate decarboxylase, Q. 3-buten-1-ol dehydratase, R. 3-hydroxypent-4-enoate dehydratase, S. vinylisomerase.
  • FIG. 3 shows pathways to but-3-enol and/or butadiene from lactoyl-CoA and acetyl-CoA. Enzymes are A. 3-Oxo-4-hydroxypentanoyl-CoA thiolase, B. 3-oxo-4-hydroxypentanoyl-CoA transferase, synthetase or hydrolase, C. 3-oxo-4-hydroxypentanoate reductase, D. 3,4-dihydroxypentanoate dehydratase, E. 4-hydroxypent-2-enoate decarboxylase, F. 3-buten-2-ol dehydratase, G. 3-oxo-4-hydroxypentanoyl-CoA reductase, H. 3,4-dihydroxypentanoyl-CoA dehydratase, I. 3,4-dihydroxypentanoyl-CoA transferase, synthetase or hydrolase, J. 4-hydroxypent-2-enoyl-CoA transferase, synthetase or hydrolase, K. 4-hydroxypent-2-enoate dehydratase, L. 2,4-pentadienoate decarboxylase, M. 4-hydroxypent-2-enoyl-CoA dehydratase, N. 2,4-pentadienoyl-CoA hydrolase, transferase or synthetase, O. vinylisomerase.
  • FIG. 4 shows pathways for converting crotyl alcohol (2-Buten-1-ol) to 3-buten-2-ol and/or butadiene. Enzymes are A. crotyl alcohol kinase, B. 2-butenyl-4-phosphate kinase, C. butadiene synthase, D. 3-buten-2-ol synthase, E. 3-buten-2-ol synthase, F. crotyl alcohol isomerase, G. 3-buten-2-ol dehydratase, H. crotyl alcohol dehydratase, I. crotyl alcohol diphosphokinase, J. butadiene synthase (from 2-butenyl-4-phosphate).
  • FIG. 5 shows the GCMS analysis of an authentic sample of MVC or 3-buten-2-ol.
  • FIG. 6 shows the GCMS chromatograms obtained for the enzymatic (blue) assay with 50 mM CrotOH after 16 hours and 1 mM standard of MVC or 3-buten-2-ol dissolved in minimal media.
  • FIG. 7 is a graph showing activity measurements for assessing the NADPH-dependent reduction of crotonaldehyde to crotonol.
  • FIG. 8 is a graph of in vitro formation of crotyl phosphate (CrPi) and ADP over time from a composition including ATP, crotyl alcohol, and hydroxyethylthiazole kinase.
    •  DETAILED DESCRIPTION OF THE INVENTION
  • The product pathways described herein are specifically contemplated for use in the organisms, compositions and their uses, such as in methods to make the target products of 1,3-butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol or 2,4-pentadienoate or other product, and other embodiments as taught herein.
  • Embodiments of the disclosure are directed to the design of metabolic pathways, and the production and use of non-naturally occurring microbial organisms capable of producing butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol and/or 2,4-pentadienoate. As disclosed herein, metabolic pathways can be designed and recombinantly engineered to achieve the biosynthesis of butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate in microbial organisms such as Escherichia coli and other cells or organisms. Biosynthetic production of butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate can be performed by construction and fermentation of strains having the designed metabolic genotype.
  • FIG. 1 provides exemplary pathways to butadiene from acetyl-CoA and pyruvate via intermediate 2-oxopent-3-enoate (step E) or 3-hydroxybutyraldehyde (step C). Also shown are pathways to the intermediate products (which may be desirably obtained as final products) of crotol alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate, which in certain pathway routes, can be intermediates in butadiene synthesis. In one pathway, acetyl-CoA is converted to acetaldehyde by acetyl-CoA reductase, and pyruvate and acetaldehyde are then converted to 4-hydroxy-2-oxovalerate by 4-hydroxy 2-oxovalerate aldolase.
  • With reference to FIG. 1, the intermediate 4-hydroxy-2-oxovalerate can be promoted to enter different pathways depending on the metabolic design. In one pathway (“E”), 4-hydroxy-2-oxovalerate can be converted to 2-oxopent-3-enoate in the presence of 4-hydroxy-2-oxovalerate 3-dehydratase. Pathway “E” can branch off into one or more different pathways depending on the metabolic design. For example, in pathway “E→N” 2-oxopent-3-enoate is converted to 2-oxopent-3-enoyl-CoA in the presence of 2-oxopent-3-enoyl-CoA synthetase or transferase (N); subsequent enzymatic conversions of intermediates can occur. As another example, in pathway “E→F” 2-oxopent-3-enoate is converted to 2-hydroxypent-3-enoate in the presence of 2-oxopent-3-enoate reductase (F); subsequent enzymatic conversions of intermediates can occur. If desired, the intermediate 2,4-pentadienoate can be obtained further downstream in the E→F branch of the pathway. As another example, in pathway “E→I” 2-oxopent-3-enoate is converted to crotyl aldehyde in the presence of 2-oxopent-3-enoate decarboxylase (I); subsequent enzymatic conversions of intermediates can occur. In desired, the intermediates such as crotol alcohol and/or 3-buten-2-ol can be obtained further downstream in the E→I branch of the pathway.
  • With reference to FIG. 1, the intermediate 4-hydroxy-2-oxovalerate can also be promoted to enter pathway (“C”), where 4-hydroxy-2-oxovalerate can be converted to 3-hydroxy butyraldehyde in the presence of 4-hydroxy 2-oxovalerate decarboxylase. Subsequently, 4-hydroxy 2-oxovalerate decarboxylase can be converted to crotyl aldehyde in the presence of 3-hydroxybutyraldehyde dehydratase (D). Crotyl aldehyde can be converted to crotyl alcohol, which can be an intermediate in the pathway, or can be obtained as a final product, by crotyl aldehyde reductase (J). Subsequently, crotyl alcohol can be converted to 3-buten-2-ol, which can be an intermediate in the pathway, or can be obtained as a final product, by crotyl alcohol vinylisomerase (K). 3-buten-2-ol from steps K or T can be isolated or from step T converted to crotyl alcohol enzymatically (W) or chemically. Crotyl alcohol can enter FIG. 4 or be isolated.
  • With reference to FIG. 1, the non-naturally occurring microbial organism can have any one of the following pathways. E; BE; ABE; EN; ENO; ENOP; ENOPQ; ENOPQH; EF; EFU; EFUV; EFUVH; EFR; EFRP; EFRPQ; EFRPQH; EFS; EFSK; EFSKL; EFSM; EFUT; EFUTL; EI; EIJ; EIJK; EIJKL; EIJM; BEN; BENO; BENOP; BENOPQ; BENOPQH; BEF; BEFU; BEFUV; BEFUVH; BEFR; BEFRP; BEFRPQ; BEFRPQH; BEF; BEFS; BEFSK; BEFSKL; BEFSM; BEFUT; BEFUTL; BEI; BEIJ; BEIJK; BEIJKL; BEIJM; ABEN; ABENO; ABENOP; ABENOPQ; ABENOPQH; ABEF; ABEFU; ABEFUV; ABEFUVH; ABEFR; ABEFRP; ABEFRPQ; ABEFRPQH; ABEF; ABEFS; ABEFSK; ABEFSKL; ABEFSM; ABEFUT; ABEFUTL; ABEI; ABEIJ; ABEIJK; ABEIJKL; ABEIJM; D; CD; BCD; ABCD; DJ; DJM; DJK; DJKL; CDJ; CDJM; CDJK; CDJKL; BCDJ; BCDJM; BCDJK; BCDJKL; ABCDJ; ABCDJM; ABCDJK; ABCDJKL; N; O; P; R; F; U; K; and each combination of any one or more of E, D, N, O, P, R, F, U and K with any one or more of steps of FIG. 1. In each of the embodiments of the above pathways where 3-buten-2-ol is produced from Step T it can be followed by Step W, conversion to crotyl alcohol. For example, EFUTW; BEFUTW; ABEFUTW.
  • In some embodiments, at least one of the pathway enzymes is encoded by nucleic acid that is heterologous to the host species. For example, the heterologous nucleic acid can be obtained from a microbial species other than the host species, such as a nucleic acid from a bacteria other than E. coli (i.e., a non-E. coli bacteria), wherein the heterologous nucleic acid is transformed into E. coli host organism to create any of the pathways of the disclosure. In some embodiments, two, three, four, five, six, etc., heterologous nucleic acids from one or more bacteria other than the host bacteria, are transformed into the bacterial host organism to create any of the pathways of the disclosure. For example, to create a pathway such as BEFUVH in a host organism, one can first determine what, if any, enzymes of the BEFUVH are naturally present in a desired host species. If B is native to the host species, but not EFUVH, the host can then be transformed with heterologous nucleic acids encoding EFUVH to create a pathway in the cell to form butadiene. This approach can be used to create a non-naturally occurring microbial organism having a pathway to butadiene, crotyl alcohol, 2,4-pentadienoate, or 3-buten-2-ol, having any of the pathways of the disclosure.
  • FIG. 2 provides exemplary pathways to butadiene from acetyl-CoA and pyruvate via intermediate 5-hydroxypent-2-enoate (step E) or 3-hydroxypent-4-enoate (step H). Also shown are pathways to the intermediate products (which may be desirably obtained as final products) of crotol alcohol, 3-buten-1-ol and/or 2,4-pentadienoate, which in certain pathway routes, can be intermediates in butadiene synthesis. In one pathway, acetyl-CoA is converted to acetaldehyde by acetyl-CoA reductase (A), and pyruvate and acetaldehyde then converted to 4-hydroxy-2-oxovalerate by 4-hydroxy 2-oxovalerate aldolase (B). 4-hydroxy-2-oxovalerate is converted to 2-oxopent-4-enoate by 4-hydroxy 2-oxovalerate dehydratase (C), and then 2-oxopent-4-enoate is converted to 2-hydroxypent-4-enoate by 2-oxopent-4-enoate reductase (D).
  • As shown in FIG. 2, the intermediate 2-hydroxypent-4-enoate can enter pathway branch “E” or “H.” In pathway branch E, 2-hydroxypent-4-enoate is converted to 5-hydroxypent-2-enoate in the presence of 2-hydroxypent-4-enoate vinylisomerase. The intermediate 5-hydroxypent-2-enoate can then enter one or more of pathway branches (P, F, and/or I) before conversion to butadiene. 3-buten-1-ol can be converted to crotyl alcohol (S), which can be an intermediate in the pathway (entering FIG. 4), or can be obtained as a final product. In pathway branch H, 2-hydroxypent-4-enoate is converted to 3-hydroxypent-4-enoate in the presence of 2-hydroxypent-4-enoate mutase. The intermediate 3-hydroxypent-4-enoate can then enter one or more of pathway branches (R, K, and/or L) before conversion to butadiene.
  • With reference to FIG. 2, the non-naturally occurring microbial organism can have any one of the following pathways. E; DE; CDE; BCDE; ABCDE; EF; EFG; EP; EPQ; EI; EIN; EINJ; EINJG; ABCDEF; ABCDEFG; ABCDEP; ABCDEPQ; ABCDEI; ABCDEIN; ABCDEINJ; ABCDEINJG; BCDEF; BCDEFG; BCDEP; BCDEPQ; BCDEI; BCDEIN; BCDEINJ; BCDEINJG; CDEF; CDEFG; CDEP; CDEPQ; CDEI; CDEIN; CDEINJ; CDEINJG; DEF; DEFG; DEP; DEPQ; DEI; DEIN; DEINJ; DEINJG; H; DH; CDH; BCDH; ABCDH; HL; HLM; HLMN; HLMNJ; HLMNJG; HLO; HLOJ; HLOJG; HLMI; HLMIF; HLMIFG; HLMIP; HLMIPQ; HR; HRG; HK; ABCDHL; ABCDHLM; ABCDHLMN; ABCDHLMNJ; ABCDHLMNJG; ABCDHLO; ABCDHLOJ; ABCDHLOJG; ABCDHLMI; ABCDHLMIF; ABCDHLMIFG; ABCDHLMIP; ABCDHLMIPQ; ABCDHR; ABCDHRG; ABCDHK; BCDHL; BCDHLM; BCDHLMN; BCDHLMNJ; BCDHLMNJG; BCDHLO; BCDHLOJ; BCDHLOJG; BCDHLMI; BCDHLMIF; BCDHLMIFG; BCDHLMIP; BCDHLMIPQ; BCDHR; BCDHRG; BCDHK; CDHL; CDHLM; CDHLMN; CDHLMNJ; CDHLMNJG; CDHLO; CDHLOJ; CDHLOJG; CDHLMI; CDHLMIF; CDHLMIFG; CDHLMIP; CDHLMIPQ; CDHR; CDHRG; CDHK; DHL; DHLM; DHLMN; DHLMNJ; DHLMNJG; DHLO; DHLOJ; DHLOJG; DHLMI; DHLMIF; DHLMIFG; DHLMIP; DHLMIPQ; DHR; DHRG; DHK; M; and each combination of any one or more of E, H, and M with any one or more of steps of FIG. 2. In each of the embodiments of the above pathways where 3-buten-1-ol is produced from Step P it can be followed by Step S, conversion to crotyl alcohol. For example, BCDEPS; CDEPS; DEPS; HLMIPS; ABCDHLMIPS; BCDHLMIPS; CDHLMIPS and DHLMIPS.
  • FIG. 3 provides exemplary pathways to butadiene from lactoyl-CoA and acetyl-CoA via intermediate 4-hydroxypent-2-enoate, 3-buten-2-ol, 4-hydroxypent-2-enoyl-CoA, 4-hydroxypent-2-enoate; enzymes such as 3,4-dihydroxypentanoate dehydratase (step D), 4-hydroxypent-2-enoate decarboxylase (step E), 3,4-dihydroxypentanoyl-CoA dehydratase (step H), and 4-hydroxypent-2-enoyl-CoA transferase (step J) can be used. 3-buten-2-ol from step E can be isolated as final product or converted to crotyl alcohol enzymatically (O) or chemically. Crotyl alcohol can be isolated or enter FIG. 4.
  • With reference to FIG. 3, the non-naturally occurring microbial organism can have any one of the following pathways: E; EF; D; DE; DEF; DK; DKL; J; JE; JEF; JK; JKL; H; HJ; HJE; HJEF; HJK; HJKL; HM; HMN; HMNL; GH; GHJ; GHJE; GHJEF; GHJK; GHJKL; GHM; GHMN; GHMIL; AGH; AGHJ; AGHJE; AGHJEF; AGHJK AGHJKL; AGHM; AGHMN; AGHMNL; ID; IDE; IDEF; IDK; IDKL; GID; GIDE; GIDEF; GIDK; GIDKL; AGID; AGIDE; AGIDEF; AGIDK; AGIDKL; CD; CDE; CDEF; CDK; CDKL; BCD; BCDE; BCDEF; BCDK; BCDKL; ABCD; ABCDE; ABCDEF; ABCDK; ABCDKL; HJ; HJE; HJEF; HJK; HJKL; GHJ; GHJE; GHJEF; GHJK GHJKL; AGHJ; AGHJE; AGHJEF; AGHJK; and AGHJKL; and each combination of any one or more of D, H, J and E with any one or more of steps of FIG. 3. In each of the embodiments of the above pathways where 3-buten-2-ol is produced it can be followed by Step O, conversion to crotyl alcohol. For example, EO; DEO; JEO; HJEO; AGHJEO; IDEO; GIDEO; AGIDEO; CDEO; BCDEO; ABCDEO; and GHJEO.
  • FIG. 4 provides exemplary pathways to butadiene from crotyl alcohol via one or more of the following intermediates: 2-butenyl-4-phosphate, 2-butenyl-4-diphosphate, and/or 3-buten-2-ol. Optionally, crotyl alcohol can be introduced into this pathway via any crotyl alcohol-producing pathway known in the art, or as described herein (e.g., from precursors crotyl aldehyde as converted to crotyl alcohol in the presence of crotyl aldehyde reductase (1J); or 2-hydroxypent-3-enoate converted to crotyl alcohol in the presence of 2-hydroxypent-3-enoate decarboxylase (1S) and including conversion from 3-buten-1-ol or 3-buten-2-ol). In some embodiments, the crotyl alcohol is produced by a pathway of FIG. 1, 2 or 3 and then introduced into a pathway of FIG. 4.
  • For example, step A of FIG. 4 uses a hydroxyethylthiazole kinase, a thiamine kinase, a pantothenate kinase, a 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, a riboflavin kinase, a L-fuculokinase, and/or a choline kinase, such as those described herein.
  • For example, step B of FIG. 4 subset of kinases of the EC 2.7.1.a class suitable for FIG. 4 Step B include 2.7.4.a Phosphokinases listed below. 2-Butenyl-4-phosphate kinase enzymes catalyze the transfer of a phosphate group to the phosphate group of 2-butenyl-4-phosphate (FIG. 4B).
  • With reference to FIG. 4, the non-naturally occurring microbial organism can have any one of the following pathways: D; DG; AD; ADG; E; EG; BE; BEG; ABE; ABEG; IE; IEG; F; FG; G; H; J; AJ; A; AB; ABC and each combination of any one or more of steps D, E, F with any one or more of steps of FIG. 4, wherein when at least one of 4A, 4B, 4C, 4G or 4H is present then (i) at least one other unique step or pathway is present, such as step 4D, 4E, any unique step or pathway from FIG. 1 (e.g. 1D, 1E, 1F), or any one or more of Step 4A, 4B, 4C, 4G or 4H or (ii) at least one of A, B, C, G or H consists of a specific sub-group of enzyme classes or enzymes described herein as A for Step A, as B for Step B, as C for Step C, as G for Step G or as H for Step H.
  • In one embodiment for each pathway or step above is also contemplated the substitution of any one or more of subsets of enzymes described as Steps 1L, 1V, 1M, 2C, 2F, 2O, 2N, 2Q, 2R, 3F, 3K, 3M, 4A, 4B, 4C, 4G and 4H for its corresponding step (e.g. replace enzymes described for Step 1L with those described for Step 1L*).
  • With reference to FIGS. 1 and 4, or FIGS. 3 and 4, crotyl alcohol or 3-buten-2-ol is produced by a pathway of FIG. 1 (e.g., a pathway such as: ABCDJ; ABEIJ; ABEFS; BCDJ; BEIJ: BEFS; CDJ; EIJ; EFS; and DJ, optionally further with step W), or is produced by a pathway of FIG. 3, and then introduced into a pathway of FIG. 4 by combining with a pathway of FIG. 4 selected from: A; AB; ABC; AJ; AD; ADG; ABE; ABEG; F; FG; I; IC; IE; IEG; H; and G. In a further specifically contemplated embodiment is pathway having substitution of any one or more of subsets of enzymes described in Steps 4G and 4H for its corresponding step (e.g. FIG. 1 ABCDJ plus FIG. 4 IEG becomes FIG. 1 ABCDJ plus FIG. 4 IEG*).
  • With reference to FIGS. 2 and 4, crotyl alcohol or 3-buten-1-ol is produced by a pathway of FIG. 2 and then introduced into a pathway of FIG. 4 by combining with a pathway of FIG. 4 selected from: A; AB; ABC; AJ; AD; ADG; ABE; ABEG; F; FG; I; IC; IE; IEG; H; and G. In a further specifically contemplated embodiment is pathway having substitution of any one or more of subsets of enzymes described in Steps 4G and 4H for its corresponding step (e.g. FIG. 2 ABCDEPS plus FIG. 4 IEG becomes FIG. 2 ABCDEPS plus FIG. 4 IEG*).
  • Also specifically contemplated herein as unique transformations of FIG. 1 in addition to Step 1D and 1E are Steps 1N, 1O, 1P, 1R, 1F, U and 1K. Also specifically contemplated herein as unique transformations of FIG. 2 in addition to Step 2H and 2E is Step 2M. Also specifically contemplated herein as unique transformations of FIG. 4 in addition to Step 4D and 4E is Step 4F. In addition, Steps 1L, 1V, 1M, 2C, 2F, 2O, 2N, 2Q, 2R, 3F, 3K, 3M, 4A, 4B, 4C, 4G and 4H are unique. Accordingly, all subject matter specifically expressed herein applies equally to these additional unique steps. For example are embodiments of a non-naturally occurring microbial organism having a pathway to butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-1-ol, 3-buten-2-ol, or other product said microbial organism comprising a nucleic acid encoding a pathway enzyme for the unique step, as well as methods of use described herein.
  • With reference to FIG. 4, a step A subset of kinases of FIG. 4 Step A include enzymes exemplified in the tables below for hydroxyethylthiazole kinase, thiamine kinase, pantothenate kinase, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, riboflavin kinase, L-fuculokinase and choline kinase. The table below provides hydroxyethyl thiazole kinases, including EC 2.7.1.50 class, for FIG. 4, Step A:
  • Protein GenBank ID GI Number Organism
    ThiM YP_007535827.1  16080881 Bacillus subtilis
    Thi6 CAA97929.1  1370444 Saccharomyces serevisiae
    ThiM NP_372616.1  15925082 Staphylococcus aureus
    PH1157, NP_143059.1  14590984 Pyrococcus horikoshii OT3
    thiM
    (analogue
    of thiK)
    ThiM Q830K4  81585041 Enterococcus faecalis V583
    ThiM YP_006701495 405760899 Streptococcus pneumoniae
    SPNA45
    ThiM YP_004888181 380031190 Lactobacillus plantarum
    WCFS1
    ThiM WP_012906431 502670591 Citrobacter rodentium
    ThiM NP_461091  16765476 Salmonella enterica
    subsp. Enterica LT2
    ThiM YP_771477 116255644 Rhizobium
    leguminosarum bv.
    viciae 3841
    ThiM AAC75165.1  1788421 Escherichia coli str.
    (b2104) K-12 substr. MG1655
  • Exemplary candidate thiamine kinases for FIG. 4, Step A are:
  • Protein GenBank ID GI Number Organism
    thiK AAC74190.1  1787349 Escherichia coli K12
    thiK NP_460178.1 16764563 Salmonella enterica subsp.
    enterica serovar
    Typhimurium str. LT2
  • Exemplary fuculokinases for FIG. 4, Step A are:
  • Protein GenBank ID GI Number Organism
    b2803 AAC75845.1  1789168 Escherichia coli K12
    MG1655
    STM14_3591 ACY90002.1 267995117 Salmonella enterica subsp.
    enterica serovar
    Typhimurium str.
    14028S
    D186_16909 EKS55716.1 411772069 Citrobacter freundii ATCC
    8090 = MTCC 1658
  • Exemplary 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase for FIG. 4, Step A are:
  • Protein GenBank ID GI Number Organism
    folK AAC73253.1  1786335 Escherichia coli K12
    folK NP_816865.1 29377711 Enterococcus faecalis V583
  • Exemplary pantothenate kinases for FIG. 4, Step A are:
  • Protein GenBank ID GI Number Organism
    CoaA YP_006514461.1 397672926 Mycobacterium
    tuberculosis H37Rv
    CoaA YP_491482.1 388479290 Escherichia coli K12
    CoaX Q9WZY5.1  81553296 Thermotoga maritima
    MSB8
    Sav2130 NP_372654.1  15925120 Staphylococcus aureus
    subsp. aureus Mu50
  • Exemplary 4-(cytidine 5′-diphospho)-2-C-methyl-D-erythritol kinases (2.7.1.148) for FIG. 4, Step A are:
  • Protein GenBank ID GI Number Organism
    ispE NP_415726.1  16129171 Escherichia coli K12
    ispE KBJ36713.1 623367758 Mycobacterium tuberculosis
    H37Rv
  • Step A subset of synthases (alkene forming) for FIG. 4 Step C include 4-dimethylallyltryptophan synthase (class EC 2.5.1.34) and dimethylallyltranstransferase (class EC 2.5.1.1) that catalyze the conversion of 2-butenyl-4-diphosphate to 1,3-butadiene (Butadiene Synthase (BDS). The enzymes in these classes naturally possess such activity or can be engineered to exhibit or enhance this activity.
  • Enzyme Commission Number Enzyme Name
    2.5.1.34 4-dimethylallyltryptophan synthase
    2.5.1.1 dimethylallyltranstransferase
  • The enzyme-subsets designated as steps 1L, 1V, 1M, 2C, 2F, 2O, 2N, 2Q, 2R, 3F, 3K, 3M, 4G and 4H of dehydratase of the EC 4.2.1.a class for their respective Steps 1L, 1V, 1M, 2C, 2F, 2O, 2N, 2Q, 2R, 3F, 3K, 3M, 4G and 4H include the exemplary dehydratases of the 4.2.1.a class shown in the table below as well as a dehydratase class that dehydrates phenyllactyl-CoA to cinnamoyl-CoA exemplified by the dehydratase found in Clostridium sporogens that dehydrates phenyllactyl-CoA to cinnamoyl-CoA. This enzyme is composed of three subunits, one of which is a CoA transferase. The first step comprises of a CoA transfer from cinnamoyl-CoA to phenyllactate leading to the formation of phenyllactyl-CoA and cinnamate. The product cinnamate is released. The dehydratase then converts phenyllactyl-CoA into cinnamoyl-CoA. The FldA is the CoA transferase and FldBC are alpha and beta subunits of the dehydratase, which are related to component D from A. fermentans.
  • GenBank
    Gene Accession No. GI No. Organism
    hgdA AAD31676.1  4883832 Clostridum symbiosum
    hgdB AAD31677.1  4883833 Clostridum symbiosum
    hgdC AAD31675.1  4883831 Clostridum symbiosum
    hgdA EDK88042.1 148322792 Fusobacterium nucleatum
    hgdB EDK88043.1 148322793 Fusobacterium nucleatum
    hgdC EDK88041.1 148322791 Fusobacterium nucleatum
    FldB Q93AL9.1  75406928 Clostridium sporogens
    FldC Q93AL8.1  75406927 Clostridium sporogens
  • FIG. 4G and FIG. 4H subsets and FIG. 1 Step L, V and M subsets and FIG. 2 Step C, F, N, O, Q and R subsets, and FIG. 3 Steps F, K and M subsets are subsets of dehydratases for their respective Steps 1L, 1V, 1M, 2C, 2F, 2O, 2N, 2Q, 2R, 3F, 3K, 3M, 4G and 4H are listed in the following table.
  • Protein GenBank ID GI Number Organism
    CGGC5_10771 ELA28661.1 429853596 Colletotrichum
    gloeosporioides
    Nara gc5
    UCRPA7_8726 EON95759.1 500251895 Togninia minima
    UCRPA7
    UCRNP2_8820 EOD44468.1 485917493 Neofusicoccum parvum
    UCRNP2
  • Further enzymes of FIG. 4G and FIG. 4H subsets and FIG. 1 Step L, V and M subsets and FIG. 2 Step C, F, N, O, Q and R subsets, and FIG. 3 Steps F, K and M subsets include dimethylmaleate hydratases that catalyze the dehydration of (2R,3S)-2,3-dimethylmalate into dimethylmaleate (EC 4.2.1.85) shown in the table below.
  • Protein GenBank ID GI Number Organism
    LeuC 4KP1_A 635576713 Methanococcus jannaschii
    phaJ1 ABP99034.1 145967354 Pseudomonas putida
  • Enzymes with dehydratase and vinylisomerase activity suitable for FIG. 1 Steps G and M subsets and FIG. 4 Steps G and H subsets include bifunctional enzymes with dehydratase and isomerase activities exemplified below.
  • Protein GenBank ID GI Number Organism
    CGGC5_10771 ELA28661.1 429853596 Colletotrichum
    gloeosporioides Nara gc5
    UCRPA7_8726 EON95759.1 500251895 Togninia minima UCRPA7
    UCRNP2_8820 EOD44468.1 485917493 Neofusicoccum parvum
    UCRNP
    2
  • As used herein, the term “non-naturally occurring” when used in reference to a microbial organism or microorganism of the invention is intended to mean that the microbial organism has at least one genetic alteration not normally found in a naturally occurring strain of the referenced species, including wild-type strains of the referenced species. Genetic alterations include, for example, modifications introducing expressible nucleic acids encoding metabolic polypeptides, other nucleic acid additions, nucleic acid deletions and/or other functional disruption of the microbial organism's genetic material. Such modifications include, for example, coding regions and functional fragments thereof, for heterologous, homologous or both heterologous and homologous polypeptides for the referenced species. Additional modifications include, for example, non-coding regulatory regions in which the modifications alter expression of a gene or operon. Exemplary metabolic polypeptides include enzymes or proteins within a butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate biosynthetic pathway.
  • A metabolic modification refers to a biochemical reaction that is altered from its naturally occurring state. Therefore, non-naturally occurring microorganisms can have genetic modifications to nucleic acids encoding metabolic polypeptides, or functional fragments thereof. Exemplary metabolic modifications are disclosed herein.
  • As used herein, the term “isolated” when used in reference to a microbial organism is intended to mean an organism that is substantially free of at least one component as the referenced microbial organism is found in nature. The term includes a microbial organism that is removed from some or all components as it is found in its natural environment. The term also includes a microbial organism that is removed from some or all components as the microbial organism is found in non-naturally occurring environments. Therefore, an isolated microbial organism is partly or completely separated from other substances as it is found in nature or as it is grown, stored or subsisted in non-naturally occurring environments. Specific examples of isolated microbial organisms include partially pure microbes, substantially pure microbes and microbes cultured in a medium that is non-naturally occurring.
  • As used herein, the terms “microbial,” “microbial organism” or “microorganism” are intended to mean any organism that exists as a microscopic cell that is included within the domains of archaea, bacteria or eukarya. Therefore, the term is intended to encompass prokaryotic or eukaryotic cells or organisms having a microscopic size and includes bacteria, archaea and eubacteria of all species as well as eukaryotic microorganisms such as yeast and fungi. The term also includes cell cultures of any species that can be cultured for the production of a biochemical.
  • As used herein, the term “CoA” or “coenzyme A” is intended to mean an organic cofactor or prosthetic group (nonprotein portion of an enzyme) whose presence is required for the activity of many enzymes (the apoenzyme) to form an active enzyme system. Coenzyme A functions in certain condensing enzymes, acts in acetyl or other acyl group transfer and in fatty acid synthesis and oxidation, pyruvate oxidation and in other acetylation.
  • As used herein, the term “substantially anaerobic” when used in reference to a culture or growth condition is intended to mean that the amount of oxygen is less than about 10% of saturation for dissolved oxygen in liquid media. The term also is intended to include sealed chambers of liquid or solid medium maintained with an atmosphere of less than about 1% oxygen.
  • “Exogenous” as it is used herein is intended to mean that the referenced molecule or the referenced activity is introduced into the host microbial organism. The molecule can be introduced, for example, by introduction of an encoding nucleic acid into the host genetic material such as by integration into a host chromosome or as non-chromosomal genetic material such as a plasmid. Therefore, the term as it is used in reference to expression of an encoding nucleic acid refers to introduction of the encoding nucleic acid in an expressible form into the microbial organism. When used in reference to a biosynthetic activity, the term refers to an activity that is introduced into the host reference organism. The source can be, for example, a homologous or heterologous encoding nucleic acid that expresses the referenced activity following introduction into the host microbial organism. Therefore, the term “endogenous” refers to a referenced molecule or activity that is present in the host. Similarly, the term when used in reference to expression of an encoding nucleic acid refers to expression of an encoding nucleic acid contained within the microbial organism. The term “heterologous” refers to a molecule or activity derived from a source other than the referenced species whereas “homologous” refers to a molecule or activity derived from the host microbial organism. Accordingly, exogenous expression of an encoding nucleic acid of the invention can utilize either or both a heterologous or homologous encoding nucleic acid.
  • It is understood that when more than one exogenous nucleic acid is included in a microbial organism that the more than one exogenous nucleic acids refers to the referenced encoding nucleic acid or biosynthetic activity, as discussed above. It is further understood, as disclosed herein, that such more than one exogenous nucleic acids can be introduced into the host microbial organism on separate nucleic acid molecules, on polycistronic nucleic acid molecules, or a combination thereof, and still be considered as more than one exogenous nucleic acid. For example, as disclosed herein a microbial organism can be engineered to express two or more exogenous nucleic acids encoding a desired pathway enzyme or protein. In the case where two exogenous nucleic acids encoding a desired activity are introduced into a host microbial organism, it is understood that the two exogenous nucleic acids can be introduced as a single nucleic acid, for example, on a single plasmid, on separate plasmids, can be integrated into the host chromosome at a single site or multiple sites, and still be considered as two exogenous nucleic acids. Similarly, it is understood that more than two exogenous nucleic acids can be introduced into a host organism in any desired combination, for example, on a single plasmid, on separate plasmids, can be integrated into the host chromosome at a single site or multiple sites, and still be considered as two or more exogenous nucleic acids, for example three exogenous nucleic acids. Thus, the number of referenced exogenous nucleic acids or biosynthetic activities refers to the number of encoding nucleic acids or the number of biosynthetic activities, not the number of separate nucleic acids introduced into the host organism.
  • The non-naturally occurring microbal organisms of the invention can contain stable genetic alterations, which refers to microorganisms that can be cultured for greater than five generations without loss of the alteration. Generally, stable genetic alterations include modifications that persist greater than 10 generations, particularly stable modifications will persist more than about 25 generations, and more particularly, stable genetic modifications will be greater than 50 generations, including indefinitely.
  • Those skilled in the art will understand that the genetic alterations, including metabolic modifications exemplified herein, are described with reference to a suitable host organism such as E. coli and their corresponding metabolic reactions or a suitable source organism for desired genetic material such as genes for a desired metabolic pathway. However, given the complete genome sequencing of a wide variety of organisms and the high level of skill in the area of genomics, those skilled in the art will readily be able to apply the teachings and guidance provided herein to essentially all other organisms. For example, the E. coli metabolic alterations exemplified herein can readily be applied to other species by incorporating the same or analogous encoding nucleic acid from species other than the referenced species. Such genetic alterations include, for example, genetic alterations of species homologs, in general, and in particular, orthologs, paralogs or nonorthologous gene displacements.
  • An ortholog is a gene or genes that are related by vertical descent and are responsible for substantially the same or identical functions in different organisms. For example, mouse epoxide hydrolase and human epoxide hydrolase can be considered orthologs for the biological function of hydrolysis of epoxides. Genes are related by vertical descent when, for example, they share sequence similarity of sufficient amount to indicate they are homologous, or related by evolution from a common ancestor. Genes can also be considered orthologs if they share three-dimensional structure but not necessarily sequence similarity, of a sufficient amount to indicate that they have evolved from a common ancestor to the extent that the primary sequence similarity is not identifiable. Genes that are orthologous can encode proteins with sequence similarity of about 25% to 100% amino acid sequence identity. Genes encoding proteins sharing an amino acid similarity less that 25% can also be considered to have arisen by vertical descent if their three-dimensional structure also shows similarities.
  • Orthologs include genes or their encoded gene products that through, for example, evolution, have diverged in structure or overall activity. For example, where one species encodes a gene product exhibiting two functions and where such functions have been separated into distinct genes in a second species, the three genes and their corresponding products are considered to be orthologs. For the production of a biochemical product, those skilled in the art will understand that the orthologous gene harboring the metabolic activity to be introduced or disrupted is to be chosen for construction of the non-naturally occurring microorganism. An example of orthologs exhibiting separable activities is where distinct activities have been separated into distinct gene products between two or more species or within a single species.
  • In contrast, paralogs are homologs related by, for example, duplication followed by evolutionary divergence and have similar or common, but not identical functions. Paralogs can originate or derive from, for example, the same species or from a different species. For example, microsomal epoxide hydrolase (epoxide hydrolase I) and soluble epoxide hydrolase (epoxide hydrolase II) can be considered paralogs because they represent two distinct enzymes, co-evolved from a common ancestor, that catalyze distinct reactions and have distinct functions in the same species. Paralogs are proteins from the same species with significant sequence similarity to each other suggesting that they are homologous, or related through co-evolution from a common ancestor.
  • A nonorthologous gene displacement is a nonorthologous gene from one species that can substitute for a referenced gene function in a different species. Substitution includes, for example, being able to perform substantially the same or a similar function in the species of origin compared to the referenced function in the different species. Although generally, a nonorthologous gene displacement will be identifiable as structurally related to a known gene encoding the referenced function, less structurally related but functionally similar genes and their corresponding gene products nevertheless will still fall within the meaning of the term as it is used herein. Functional similarity requires, for example, at least some structural similarity in the active site or binding region of a nonorthologous gene product compared to a gene encoding the function sought to be substituted. Therefore, a nonorthologous gene includes, for example, a paralog or an unrelated gene.
  • Therefore, in identifying and constructing the non-naturally occurring microbial organisms of the invention having biosynthetic capability, those skilled in the art will understand with applying the teaching and guidance provided herein to a particular species that the identification of metabolic modifications can include identification and inclusion or inactivation of orthologs. To the extent that paralogs and/or nonorthologous gene displacements are present in the referenced microorganism that encode an enzyme catalyzing a similar or substantially similar metabolic reaction, those skilled in the art also can utilize these evolutionally related genes.
  • Orthologs, paralogs and nonorthologous gene displacements can be determined by methods well known to those skilled in the art. For example, inspection of nucleic acid or amino acid sequences for two polypeptides will reveal sequence identity and similarities between the compared sequences. Based on such similarities, one skilled in the art can determine if the similarity is sufficiently high to indicate the proteins are related through evolution from a common ancestor. Algorithms well known to those skilled in the art, such as Align, BLAST, Clustal W and others compare and determine a raw sequence similarity or identity, and also determine the presence or significance of gaps in the sequence which can be assigned a weight or score. Such algorithms also are known in the art and are similarly applicable for determining nucleotide sequence similarity or identity. Parameters for sufficient similarity to determine relatedness are computed based on well known methods for calculating statistical similarity, or the chance of finding a similar match in a random polypeptide, and the significance of the match determined. A computer comparison of two or more sequences can, if desired, also be optimized visually by those skilled in the art. Related gene products or proteins can be expected to have a high similarity, for example, 25% to 100% sequence identity. Proteins that are unrelated can have an identity which is essentially the same as would be expected to occur by chance, if a database of sufficient size is scanned (about 5%). Sequences between 5% and 24% may or may not represent sufficient homology to conclude that the compared sequences are related. Additional statistical analysis to determine the significance of such matches given the size of the data set can be carried out to determine the relevance of these sequences.
  • Exemplary parameters for determining relatedness of two or more sequences using the BLAST algorithm, for example, can be as set forth below. Briefly, amino acid sequence alignments can be performed using BLASTP version 2.0.8 (Jan. 5, 1999) and the following parameters: Matrix: 0 BLOSUM62; gap open: 11; gap extension: 1; x_dropoff: 50; expect: 10.0; wordsize: 3; filter: on. Nucleic acid sequence alignments can be performed using BLASTN version 2.0.6 (Sep. 16, 1998) and the following parameters: Match: 1; mismatch: −2; gap open: 5; gap extension: 2; x_dropoff: 50; expect: 10.0; wordsize: 11; filter: off. Those skilled in the art will know what modifications can be made to the above parameters to either increase or decrease the stringency of the comparison, for example, and determine the relatedness of two or more sequences.
  • It is understood that any of the pathways disclosed herein, as described in the Examples and exemplified in the pathways of FIGS. 1-4, can be utilized to generate a non-naturally occurring microbial organism that produces any pathway intermediate or product, as desired. As disclosed herein, such a microbial organism that produces an intermediate can be used in combination with another microbial organism expressing downstream pathway enzymes to produce a desired product. However, it is understood that a non-naturally occurring microbial organism that produces a butadiene pathway intermediate can be utilized to produce the intermediate as a desired product.
  • This invention is also directed, in part to engineered biosynthetic pathways to improve carbon flux through a central metabolism intermediate en route to butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate. The present invention provides non-naturally occurring microbial organisms having one or more exogenous genes encoding enzymes that can catalyze various enzymatic transformations en route to butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate. In some embodiments, these enzymatic transformations are used to improve product yields, including but not limited to, from carbohydrate-based carbon feedstock.
  • The one or more exogenous genes encoding enzymes that can catalyze various enzymatic transformations en route to butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate can be used in combination with genetic modifications that improve the amount of reducing equivalents available to the biosynthetic pathways, or that minimize loss of reducing equivalents and/or carbon to byproducts. In accordance with some embodiments, the one or more exogenous genes encoding enzymes that can catalyze various enzymatic transformations en route to butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate can be used in combination with genetic modifications that (i) enhance carbon fixation, and/or (ii) accessing additional reducing equivalents from carbon sources.
  • Reducing equivalents can come in the form of NADH, NADPH, FADH, reduced quinones, reduced ferredoxins, reduced flavodoxins and thioredoxins.
  • It is understood by those skilled in the art that the pathways described herein for increasing product yield can be combined with any of the pathways disclosed herein, including those pathways depicted in the figures. One skilled in the art will understand that, depending on the pathway to a desired product and the precursors and intermediates of that pathway, a particular pathway for improving product yield, as discussed herein and in the examples, or combination of such pathways, can be used in combination with a pathway to a desired product to increase the yield of that product or a pathway intermediate.
  • The invention is described herein with general reference to the metabolic reaction, reactant or product thereof, or with specific reference to one or more nucleic acids or genes encoding an enzyme associated with or catalyzing, or a protein associated with, the referenced metabolic reaction, reactant or product. Unless otherwise expressly stated herein, those skilled in the art will understand that reference to a reaction also constitutes reference to the reactants and products of the reaction. Similarly, unless otherwise expressly stated herein, reference to a reactant or product also references the reaction, and reference to any of these metabolic constituents also references the gene or genes encoding the enzymes that catalyze or proteins involved in the referenced reaction, reactant or product. Likewise, given the well-known fields of metabolic biochemistry, enzymology and genomics, reference herein to a gene or encoding nucleic acid also constitutes a reference to the corresponding encoded enzyme and the reaction it catalyzes or a protein associated with the reaction as well as the reactants and products of the reaction.
  • The non-naturally occurring microbial organisms of the invention can be produced by introducing expressible nucleic acids encoding one or more of the enzymes or proteins participating in one or more butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate biosynthetic pathways. Depending on the host microbial organism chosen for biosynthesis, nucleic acids for some or all of a particular butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate acid biosynthetic pathway can be expressed. For example, if a chosen host is deficient in one or more enzymes or proteins for a desired biosynthetic pathway, then expressible nucleic acids for the deficient enzyme(s) or protein(s) are introduced into the host for subsequent exogenous expression. Alternatively, if the chosen host exhibits endogenous expression of some pathway genes, but is deficient in others, then an encoding nucleic acid is needed for the deficient enzyme(s) or protein(s) to achieve butadiene, crotyl alcohol, 3-buten-1-ol, 3-buten-2-ol, and/or 2,4-pentadienoate biosynthesis. Thus, a non-naturally occurring microbial organism of the invention can be produced by introducing exogenous enzyme or protein activities to obtain a desired biosynthetic pathway or a desired biosynthetic pathway can be obtained by introducing one or more exogenous enzyme or protein activities that, together with one or more endogenous enzymes or proteins, produces a desired product such as butadiene.
  • Host microbial organisms can be selected from, and the non-naturally occurring microbial organisms generated in, for example, bacteria, yeast, fungus or any of a variety of other microorganisms applicable to fermentation processes. Exemplary bacteria include species selected from Escherichia coli, Klebsiella oxytoca, Anaerobiospirillum succiniciproducens, Actinobacillus succinogenes, Mannheimia succiniciproducens, Rhizobium etli, Bacillus subtilis, Corynebacterium glutamicum, Gluconobacter oxydans, Zymomonas mobilis, Lactococcus lactis, Lactobacillus plantarum, Streptomyces coelicolor, Clostridium acetobutylicum, Pseudomonas fluorescens, and Pseudomonas putida. Exemplary yeasts or fungi include species selected from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis, Kluyveromyces marxianus, Aspergillus terreus, Aspergillus niger, Pichia pastoris, Rhizopus arrhizus, Rhizopus oryzae, Yarrowia lipolytica, and the like. E. coli is a particularly useful host organism since it is a well characterized microbial organism suitable for genetic engineering. Other particularly useful host organisms include yeast such as Saccharomyces cerevisiae. It is understood that any suitable microbial host organism can be used to introduce metabolic and/or genetic modifications to produce a desired product.
  • Examplary E. coli host organisms include any non-pathogenic E. coli strain, including E. coli strains falling within taxonomic lineages such as A, B1, and B2. E. coli K-12 strains are in subgroup A. Host organisms include derivatives and variants of E. coli K-12, such as W3110 and MG1655. See, for example, Kuhnert, P., et at. (1995) Rapid and accurate identification of Escherichia coli K-12 strains. Applied and Environmental Microbiology 61:4135-4139; Bachmann, B. J. (1972) Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol. Rev. 36 525-57; and Bachmann, B. J. Derivations and genotypes of some mutant derivatives of Escherichia coli K-12. In: Neidhardt, F. C. et al. (1996) Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology (ASM Press, Washington, D.C.)
  • In some embodiments, a heterologous nucleic acid encoding a pathway enzyme of the disclosure can be described as obtained from an organism (such as a bacteria) that is other than the organisms of the host group. For example, the heterologous nucleic acid can be from a bacterial organism other than an organism selected from the group consisting of E. coli, K. oxytoca, A. succiniciproducens, etc.
  • In particularly useful embodiments, exogenous expression of the encoding nucleic acids is employed. Exogenous expression confers the ability to custom tailor the expression and/or regulatory elements to the host and application to achieve a desired expression level that is controlled by the user. However, endogenous expression also can be utilized in other embodiments such as by removing a negative regulatory effector or induction of the gene's promoter when linked to an inducible promoter or other regulatory element. Thus, an endogenous gene having a naturally occurring inducible promoter can be up-regulated by providing the appropriate inducing agent, or the regulatory region of an endogenous gene can be engineered to incorporate an inducible regulatory element, thereby allowing the regulation of increased expression of an endogenous gene at a desired time. Similarly, an inducible promoter can be included as a regulatory element for an exogenous gene introduced into a non-naturally occurring microbial organism.
  • Sources of encoding nucleic acids for a butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol and/or 2,4-pentadienoate pathway enzyme or protein can include, for example, any species where the encoded gene product is capable of catalyzing the referenced reaction. Such species include both prokaryotic and eukaryotic organisms including, but not limited to, bacteria, including archaea and eubacteria, and eukaryotes, including yeast, plant, insect, animal, and mammal, including human. Exemplary species for such sources include, for example, Escherichia species, including Escherichia coli, Escherichia fergusonii, Methanocaldococcus jannaschii, Leptospira interrrogans, Geobacter sultrreducens, Chloroflexus aurantiacus, Roseiflexus sp. RS-1, Chloroflexus aggregans, Achromobacter xylosoxydans, Clostrdia species, including Clostridium kluyveri, Clostridium symbiosum, Clostridium acetobutylicum, Clostridium saccharoperbutylacetonicum, Clostridium ljungdahlii, Trichomonas vaginalis G3, Trypanosoma brucei, Acidaminococcus fermentans, Fusobacterium species, including Fusobacterium nucleatum, Fusobacterium mortiferum, Corynebacterium glutamicum, Rattus norvegicus, Homo sapiens, Saccharomyces species, including Saccharomyces cerevisiae, Apsergillus species, including Aspergillus terreus, Aspergillus oryzae, Aspergillus niger, Gibberella zeae, Pichia stipitis, Mycobacterium species, including Mycobacterium smegmatis, Mycobacterium avium, including subsp. pratuberculosis, Salinispora arenicola Pseudomonas species, including Pseudomonas sp. CF600, Pseudomonas putida, Pseudomonas fluorescens, Pseudomonas aeruginosa, Ralstonia species, including Ralstonia eutropha, Ralstonia eutropha JMP134, Ralstonia eutropha H16, Ralstonia pickettii, Lactobacillus plantarum, Klebsiella oxytoca, Bacillus species, including Bacillus subtilis, Bacillus pumilus, Bacillus megaterium, Pedicoccus pentosaceus, Chlorofexus species, including Chloroflexus aurantiacus, Chloroflexus aggregans, Rhodobacter sphaeroides, Methanocaldococcus jannaschii, Leptospira interrrogans, Candida maltosa, Salmonella species, including Salmonella enterica serovar Typhimurium, Shewanella species, including Shewanella oneidensis, Shewanella sp. MR-4, Alcaligenes faecalis, Geobacillus stearothermophilus, Serratia marcescens, Vibrio cholerae, Eubacterium barkeri, Bacteroides capillosus, Archaeoglobus fulgidus, Archaeoglobus fulgidus, Haloarcula marismortui, Pyrobaculum aerophilum str. IM2, Rhizobium species, including Rhizobium leguminosarum, as well as other exemplary species disclosed herein or available as source organisms for corresponding genes. However, with the complete genome sequence available for now more than 550 species (with more than half of these available on public databases such as the NCBI), including 395 microorganism genomes and a variety of yeast, fungi, plant, and mammalian genomes, the identification of genes encoding the requisite butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate biosynthetic activity for one or more genes in related or distant species, including for example, homologues, orthologs, paralogs and nonorthologous gene displacements of known genes, and the interchange of genetic alterations between organisms is routine and well known in the art. Accordingly, the metabolic alterations allowing biosynthesis of butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate described herein with reference to a particular organism such as E. coli can be readily applied to other microorganisms, including prokaryotic and eukaryotic organisms alike. Given the teachings and guidance provided herein, those skilled in the art will know that a metabolic alteration exemplified in one organism can be applied equally to other organisms.
  • In some instances, such as when an alternative butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate biosynthetic pathway exists in an unrelated species butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate biosynthesis can be conferred onto the host species by, for example, exogenous expression of a paralog or paralogs from the unrelated species that catalyzes a similar, yet non-identical metabolic reaction to replace the referenced reaction. Because certain differences among metabolic networks exist between different organisms, those skilled in the art will understand that the actual gene usage between different organisms may differ. However, given the teachings and guidance provided herein, those skilled in the art also will understand that the teachings and methods of the invention can be applied to all microbial organisms using the cognate metabolic alterations to those exemplified herein to construct a microbial organism in a species of interest that will synthesize butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol and/or 2,4-pentadienoate.
  • Methods for constructing and testing the expression levels of a non-naturally occurring butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol and/or 2,4-pentadienoate-producing host can be performed, for example, by recombinant and detection methods well known in the art. Such methods can be found described in, for example, Sambrook et al., Molecular Cloning: A Laboratory Manual, Third Ed., Cold Spring Harbor Laboratory, New York (2001); and Ausubel et al., Current Protocols in Molecular Biology, John Wiley and Sons, Baltimore, Md. (1999).
  • Exogenous nucleic acid sequences involved in a pathway for production of butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol and/or 2,4-pentadienoate can be introduced stably or transiently into a host cell using techniques well known in the art including, but not limited to, conjugation, electroporation, chemical transformation, transduction, transfection, and ultrasound transformation. For exogenous expression in E. coli or other prokaryotic cells, some nucleic acid sequences in the genes or cDNAs of eukaryotic nucleic acids can encode targeting signals such as an N-terminal mitochondrial or other targeting signal, which can be removed before transformation into prokaryotic host cells, if desired. For example, removal of a mitochondrial leader sequence led to increased expression in E. coli (Hoffmeister et al., J Biol. Chem. 280:4329-4338 (2005)). For exogenous expression in yeast or other eukaryotic cells, genes can be expressed in the cytosol without the addition of leader sequence, or can be targeted to mitochondrion or other organelles, or targeted for secretion, by the addition of a suitable targeting sequence such as a mitochondrial targeting or secretion signal suitable for the host cells. Thus, it is understood that appropriate modifications to a nucleic acid sequence to remove or include a targeting sequence can be incorporated into an exogenous nucleic acid sequence to impart desirable properties. Furthermore, genes can be subjected to codon optimization with techniques well known in the art to achieve optimized expression of the proteins.
  • An expression vector or vectors can be constructed to include one or more butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate biosynthetic pathway encoding nucleic acids as exemplified herein operably linked to expression control sequences functional in the host organism. Expression vectors applicable for use in the microbial host organisms of the invention include, for example, plasmids, phage vectors, viral vectors, episomes and artificial chromosomes, including vectors and selection sequences or markers operable for stable integration into a host chromosome. Additionally, the expression vectors can include one or more selectable marker genes and appropriate expression control sequences. Selectable marker genes also can be included that, for example, provide resistance to antibiotics or toxins, complement auxotrophic deficiencies, or supply critical nutrients not in the culture media. Expression control sequences can include constitutive and inducible promoters, transcription enhancers, transcription terminators, and the like which are well known in the art. When two or more exogenous encoding nucleic acids are to be co-expressed, both nucleic acids can be inserted, for example, into a single expression vector or in separate expression vectors. For single vector expression, the encoding nucleic acids can be operationally linked to one common expression control sequence or linked to different expression control sequences, such as one inducible promoter and one constitutive promoter. The transformation of exogenous nucleic acid sequences involved in a metabolic or synthetic pathway can be confirmed using methods well known in the art. Such methods include, for example, nucleic acid analysis such as Northern blots or polymerase chain reaction (PCR) amplification of mRNA, or immunoblotting for expression of gene products, or other suitable analytical methods to test the expression of an introduced nucleic acid sequence or its corresponding gene product. It is understood by those skilled in the art that the exogenous nucleic acid is expressed in a sufficient amount to produce the desired product, and it is further understood that expression levels can be optimized to obtain sufficient expression using methods well known in the art and as disclosed herein.
  • The invention additionally provides methods of producing butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate using the microbial organisms of the invention comprising one or more pathway gene(s). In a particular embodiment, the invention provides a method for producing a target compound by culturing a non-naturally occurring microbial organism, comprising a microbial organism having a butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate pathway comprising at least one exogenous nucleic acid encoding a pathway enzyme expressed in a sufficient amount to produce butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate, under conditions and for a sufficient period of time.
  • Suitable purification and/or assays to test for the production of producing butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate can be performed using well known methods. Suitable replicates such as triplicate cultures can be grown for each engineered strain to be tested. For example, product and byproduct formation in the engineered production host can be monitored. The final product and intermediates, and other organic compounds, can be analyzed by methods such as HPLC (High Performance Liquid Chromatography), GC-MS (Gas Chromatography-Mass Spectroscopy) and LC-MS (Liquid Chromatography-Mass Spectroscopy) or other suitable analytical methods using routine procedures well known in the art. The release of product in the fermentation broth can also be tested with the culture supernatant. Byproducts and residual glucose can be quantified by HPLC using, for example, a refractive index detector for glucose and alcohols, and a UV detector for organic acids (Lin et al., Biotechnol. Bioeng. 90:775-779 (2005)), or other suitable assay and detection methods well known in the art. The individual enzyme or protein activities from the exogenous DNA sequences can also be assayed using methods well known in the art.
  • The butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate can be separated from other components in the culture using a variety of methods well known in the art. Such separation methods include, for example, extraction procedures as well as methods that include continuous liquid-liquid extraction, pervaporation, membrane filtration, membrane separation, reverse osmosis, electrodialysis, distillation, crystallization, centrifugation, extractive filtration, ion exchange chromatography, size exclusion chromatography, adsorption chromatography, and ultrafiltration. All of the above methods are well known in the art.
  • Any of the non-naturally occurring microbial organisms described herein can be cultured to produce and/or secrete the biosynthetic products of the invention. For example, the butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate producers can be cultured for the biosynthetic production of butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate.
  • For the production of butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate, the recombinant strains are cultured in a medium with carbon source and other essential nutrients. It is sometimes desirable and can be highly desirable to maintain anaerobic conditions in the fermenter to reduce the cost of the overall process. Such conditions can be obtained, for example, by first sparging the medium with nitrogen and then sealing the flasks with a septum and crimp-cap. For strains where growth is not observed anaerobically, microaerobic or substantially anaerobic conditions can be applied by perforating the septum with a small hole for limited aeration. Exemplary anaerobic conditions have been described previously and are well-known in the art. Exemplary aerobic and anaerobic conditions are described, for example, in United State publication 2009/0047719, filed Aug. 10, 2007. Fermentations can be performed in a batch, fed-batch or continuous manner, as disclosed herein.
  • If desired, the pH of the medium can be maintained at a desired pH, in particular neutral pH, such as a pH of around 7 by addition of a base, such as NaOH or other bases, or acid, as needed to maintain the culture medium at a desirable pH. The growth rate can be determined by measuring optical density using a spectrophotometer (600 nm), and the glucose uptake rate by monitoring carbon source depletion over time.
  • The growth medium can include, for example, any carbohydrate source which can supply a source of carbon to the non-naturally occurring microorganism. Such sources include, for example, sugars such as glucose, xylose, arabinose, galactose, mannose, fructose, sucrose and starch. Other sources of carbohydrate include, for example, renewable feedstocks and biomass. Exemplary types of biomasses that can be used as feedstocks in the methods of the invention include cellulosic biomass, hemicellulosic biomass and lignin feedstocks or portions of feedstocks. Such biomass feedstocks contain, for example, carbohydrate substrates useful as carbon sources such as glucose, xylose, arabinose, galactose, mannose, fructose and starch. Other non-carbohydrate feedstocks include alcohols such as methanol, ethanol and glycerol and gaseous carbon substrates such as methane and syngas. Given the teachings and guidance provided herein, those skilled in the art will understand that renewable feedstocks and biomass other than those exemplified above also can be used for culturing the microbial organisms of the invention for the production of butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate.
  • In some embodiments, culture conditions include anaerobic or substantially anaerobic growth or maintenance conditions. Exemplary anaerobic conditions have been described previously and are well known in the art. Exemplary anaerobic conditions for fermentation processes are described herein and are described, for example, in U.S. publication 2009/0047719, filed Aug. 10, 2007. Any of these conditions can be employed with the non-naturally occurring microbial organisms as well as other anaerobic conditions well known in the art. Under such anaerobic or substantially anaerobic conditions, butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate producers can synthesize butadiene, crotyl alcohol, 3-buten-1-ol, 3-buten-2-ol, and/or 2,4-pentadienoate at intracellular concentrations of 5-10 mM or more as well as all other concentrations exemplified herein. It is understood that, even though the above description refers to intracellular concentrations, butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate producing microbial organisms can butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate intracellularly and/or secrete the product into the culture medium.
  • In addition to the culturing and fermentation conditions disclosed herein, growth condition for achieving biosynthesis of butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate can include the addition of an osmoprotectant to the culturing conditions. In certain embodiments, the non-naturally occurring microbial organisms of the invention can be sustained, cultured or fermented as described herein in the presence of an osmoprotectant. Briefly, an osmoprotectant refers to a compound that acts as an osmolyte and helps a microbial organism as described herein survive osmotic stress. Osmoprotectants include, but are not limited to, betaines, amino acids, and the sugar trehalose. Non-limiting examples of such are glycine betaine, proline betaine, dimethylthetin, dimethylsulfoniopropionate, 3-dimethylsulfonio-2-methylpropionate, pipecolic acid, dimethylsulfonioacetate, choline, L-carnitine and ectoine. In one aspect, the osmoprotectant is glycine betaine.
  • In some embodiments, the carbon feedstock and other cellular uptake sources such as phosphate, ammonia, sulfate, chloride and other halogens can be chosen to alter the isotopic distribution of the atoms present in butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate or any pathway intermediate. The various carbon feedstock and other uptake sources enumerated above will be referred to herein, collectively, as “uptake sources.”
  • As used herein, the term “bioderived” means derived from or synthesized by a biological organism and can be considered a renewable resource since it can be generated by a biological organism. Such a biological organism, in particular the microbial organisms of the invention disclosed herein, can utilize feedstock or biomass, such as, sugars or carbohydrates obtained from an agricultural, plant, bacterial, or animal source. Alternatively, the biological organism can utilize atmospheric carbon. As used herein, the term “biobased” means a product as described above that is composed, in whole or in part, of a bioderived compound of the invention. A biobased or bioderived product is in contrast to a petroleum derived product, wherein such a product is derived from or synthesized from petroleum or a petrochemical feedstock.
  • The culture conditions can include, for example, liquid culture procedures as well as fermentation and other large scale culture procedures. As described herein, particularly useful yields of the biosynthetic products of the invention can be obtained under anaerobic or substantially anaerobic culture conditions.
  • As described herein, one exemplary growth condition for achieving biosynthesis of butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate includes anaerobic culture or fermentation conditions. In certain embodiments, the non-naturally occurring microbial organisms of the invention can be sustained, cultured or fermented under anaerobic or substantially anaerobic conditions. Anaerobic conditions refer to an environment devoid of oxygen.
  • The culture conditions described herein can be scaled up and grown continuously for manufacturing of butadiene, crotyl alcohol, 3-buten-2-ol, 3-buten-1-ol, and/or 2,4-pentadienoate. Exemplary growth procedures include, for example, fed-batch fermentation and batch separation; fed-batch fermentation and continuous separation, or continuous fermentation and continuous separation.
    • Example 1. Enzyme Candidates for FIG. 1-3
  • Several pathways are shown in FIGS. 1-2 for converting pyruvate and acetaldehyde to butadiene and butadiene precursors. Acetaldehyde is formed via reduction of acetyl-CoA by acetyl-CoA reductase, also called acetaldehyde dehydrogenase (Step 1A and 2A). Pyruvate and acetaldehyde are condensed to 4-hydroxy-2-oxovalerate by 4-hydroxy-2-ketovalerate aldolase (Step 1B and 2B). Alternately, the acetaldehyde intermediate can be formed by other enzymes or metabolic pathways known in the art, such as pyruvate decarboxylase. In a particularly preferred embodiment, Steps A and B of FIGS. 1 and 2 are catalyzed by a bifunctional enzyme with aldolase and dehydrogenase activities.
  • In FIG. 1, the 4-hydroxy-2-oxovalerate product is subsequently dehydrated to 2-oxopent-3-enoate (Step 1E). Reduction of 2-oxopent-3-enoate to its corresponding hydroxyacid (Step 1F) is catalyzed by a secondary alcohol dehydrogenase. Isomerization of 2-hydroxypent-3-enoate to 4-hydroxypent-2-enoate (1U), followed by dehydration (1V) yields 2,4-pentadienoate. These two reactions can be catalyzed by two different enzymes (1U, 1V) or by a bifunctional isomerase/dehydratase (1G). Decarboxylation of 2,4-pentadienoate to butadiene is catalyzed by a pentadienoate decarboxylase (1H). Alternately, the conversion of the 2-oxopent-3-enoate or 2-hydroxypent-3-enoate intermediates to 2,4-pentadienoate proceeds via acyl-CoA intermediates (Steps 1N, 1O, 1P, 1Q, 1R). The conversion of an acid to an acyl-CoA is catalyzed by CoA transferases and CoA synthetases. Acyl-CoA to acid conversion is catalyzed by CoA synthetases, transferases or hydrolases. In yet another alternate pathway, decarboxylation of 4-hydroxy-2-oxovalerate by a keto-acid decarboxylase yields 3-hydroxybutyraldehyde (1C). This intermediate can be dehydrated to crotyl aldehyde by a dehydratase and subsequently reduced to crotyl alcohol (1J). Crotyl alcohol can be isolated as a useful product or optionally be further converted to butadiene by one or more enzymes with dehydratase and vinylisomerase activities (1K, 1L, 1M). Additional alternative routes from the 2-oxopent-3-enoate, 2-hydroxypent-3-enoate or 4-hydroxypent-2-enoate intermediates to butadiene shown in FIG. 1 take advantage of enzymes in the decarboxylase class (EC 4.1.1.-; Steps 1C, 1I, 1S, 1T). In addition to butadiene, useful products described herein include crotyl alcohol, 3-buten-2-ol, 3-hydroxybutyrate (resulting from oxidation of 3-hydroxybutyraldehyde), crotonate (oxidation of crotyl aldehyde), 4-hydroxy-2-oxovalerate, 2-oxopent-3-enoate, 2-hydroxypent-3-enoate, 4-hydroxypent-2-enoate and pentadienoate. Enzymes for catalyzing each step are described below.
  • FIG. 2 also shows pathways derived from the 4-hydroxy 2-oxovalerate intermediate. 4-hydroxy 2-oxovalerate is dehydrated to 2-oxopent-4-enoate (also called 2-hydroxypenta-2,4-dienoate) by 4-hydroxy-2-oxopentanoate dehydratase, also called 2-oxopent-4-enoate hydratase (2C). An alcohol dehydrogenase with 2-oxopent-4-enoate reductase activity forms 2-hydroxypent-4-enoate. Multiple enzymatic routes are shown in FIG. 2 for converting 2-hydroxypent-4-enoate to butadiene and butadiene precursors. One route entails isomerization of 2-hydroxypent-4-enoate to 5-hydroxypent-2-enoate (2E). This intermediate is then decarboxylated to 3-buten-1-ol (2P) and dehydrated to butadiene (2Q). Alternately, the 5-hydroxypent-2-enoate is further dehydrated to pentadienoate (2F) and subsequently decarboxylated to butadiene (2G). In yet another pathway, the 5-hydroxypent-2-enoate intermediate is activated to its corresponding acyl-CoA (2I), dehydrated to pentadienoyl-CoA (2N), and further hydrolyzed to 2,4-pentadienoate (2J). Yet another set of pathways results when 2-hydroxypent-4-enoate is converted to 3-hydroxypent-4-enoate (abbreviated as 3HPE) by a mutase (2H). Direct conversion of 3HPE to butadiene is catalyzed by an oxidative decarboxylase (2K). The two-step conversion of 3HPE to butadiene via 2,4-pentadienoate entails dehydration (2R) followed by decarboxylation (2G). In yet another route, 3HPE is activated to its acyl-CoA by a CoA synthetase or transferase. Conversion of 3-HPE-CoA to 2,4-pentadienoyl-CoA is catalyzed by an acyl-CoA dehydratase (2O) or an isomerase and dehydratase (2M, 2N). Useful products shown in FIG. 2 include butadiene, 3-buten-1-ol, 4-hydroxy-2-oxovalerate, 2-oxopent-4-enoate, 2-hydroxypent-4-enoate, 4-hydroxypent-2-enoate, 3HPE and 2,4-pentadienoate. Enzymes for catalyzing each step are described below.
  • Pathways converting lactoyl-CoA to 4-hydroxypent-2-enoate, and further to 3-buten-2-ol and butadiene, are shown in FIG. 3. The conversion of lactoyl-CoA to 4-hydroxypent-2-enoate is accomplished in four or more enzymatic steps shown in FIG. 3. Lactoyl-CoA and acetyl-CoA are first condensed to 3-oxo-4-hydroxypentanoyl-CoA by 3-oxo-4-hydroxypentanoyl-CoA thiolase, a beta-ketothiolase (Step 3A). In one pathway, the 3-oxo-4-hydroxypentanoyl-CoA intermediate is converted to its corresponding acid by a CoA hydrolase, transferase or synthetase (3B). Reduction of the 3-oxo ketone by an alcohol dehydrogenase yields 3,4-dihydroxypentanoate (3C). Dehydration of the dihydroxyacid yields 4-hydroxypent-2-enoate (3D). 4-Hydroxypent-2-enoate decarboxylase converts 4-hydroxypent-2-enoate to 3-buten-2-ol (3E). Isomerization of 3-buten-2-ol to butadiene is catalyzed by a vinylisomerase (3F). Decarboxylation and dehydration of 4-hydroxypent-2-enoate to butadiene can instead proceed with dehydration first (3K) followed by decarboxylation of 2,4-pentadienoate (3L). Further alternate pathways shown in FIG. 3 entail reduction of the 3-oxo-4-hydroxypentanoyl-CoA to its 3-hydroxyacyl-CoA (3G), and optional dehydration of the 3-hydroxyacyl-CoA to 4-hydroxypent-2-enoyl-CoA (3H). Conversion of the acyl-CoA intermediates to their corresponding acids by CoA hydrolases, synthases and transferases is shown in steps 3I and 3J. Useful products shown in FIG. 3 include butadiene, 3-buten-2-ol, 3-oxo-4-hydroxypentanoate, 3,4-dihydroxypentanoate and 4-hydroxypentanoate. Enzymes and gene candidates for catalyzing but-3-en-2-ol and butadiene pathway reactions are described in further detail below
  • FIG. 4 shows enzymatic pathways for converting CrotOH to butadiene. In one pathway, CrotOH is phosphorylated to 2-butenyl-4-phosphate by a CrotOH kinase (Step A). The 2-butenyl-4-phosphate intermediate is again phosphorylated to 2-butenyl-4-diphosphate (Step B). A butadiene synthase (BDS) enzyme catalyzes the conversion of 2-butenyl-4-diphosphate to butadiene (Step C). Such a BDS can be derived from a phosphate lyase enzyme such as isoprene synthase using methods, such as directed evolution, as described herein. In an alternate pathway, CrotOH is directly converted to 2-butenyl-4-diphosphate by a diphosphokinase (step I). In yet another alternative pathway, CrotOH can be converted to butadiene by a CrotOH dehydratase or a bifunctional dehydratase/isomerase (step H). In yet another pathway, the 2-butenyl-4-phosphate intermediate is directly converted to butadiene by a BDS (monophosphate) (step J). Further are shown pathways that proceed through a 3-buten-2-ol (MVC) intermediate. Crotyl alcohol is isomerized to MC by an enzyme with vinylisomerase activity (step F). 3-Buten-2-ol synthase enzymes catalyze the conversion of 2-butenyl-4-phosphate or 2-butenyl-4-diphosphate to 3-buten-2-ol (Steps 4D and 4E, respectively). The 3-buten-2-ol intermediate is then dehydrated to butadiene (4G).
  • EC Description Step
    1.1.1.a Alcohol dehydrogenase 1F, 1J, 1O, 2D, 3C, 3G
    1.2.1.b Acyl-CoA reductase 1A, 2A
    (aldehyde forming)
    2.3.1.a Thiolase 3A
    2.7.1.a Kinase 4A
    2.7.4.a Phosphokinase 4B
    2.7.6.a Diphosphokinase 4I
    2.8.3.a CoA transferase 1N, 1R, 1Q, 2I, 2J, 2L, 3B, 3I,
    3J, 3N
    3.2.1.a CoA hydrolase 1Q, 2J, 2I, 3B, 3I, 3J, 3N
    4.1.1.a Decarboxylase 1C, 1H, 1I, 1S, 1T, 2G, 2P,
    3E, 3L
    4.1.1.b Decarboxylase (alkene 2K
    forming)
    4.1.2.a Aldolase 1B, 2B
    4.2.1.a Dehydratase 1D, 1E, 1L, 1P, 1V, 1M
    2C, 2F, 2O, 2N, 2Q, 2R, 3D,
    3F, 3H, 3K, 3M, 4G, 4H
    4.2.1.c Dehydratase/vinylisomerase 1G, 1M, 4H
    4.2.3.a Synthase (alkene-forming) 4C
    5.3.3 Isomerase 1K, 1U, 2M, 1W, 2S, 3O
    6.2.1.a CoA synthetase 1N, 1R, 1Q, 2I, 2J, 2L, 3B, 3I,
    3J, 3N
    5.4.4 Alcohol mutase 2H, 2E
    Alkenol Synthases 4D, 4E
    • 1.1.1.a Alcohol Dehydrogenase
  • The enzyme activities required for the reactions shown in FIGS. 1-4 are listed in the table and described in further detail below. The reduction of 2-oxopent-3-enoate, 2-oxopent-4-enoate to corresponding 2-hydroxyacids (1F, 2D) are catalyzed by secondary alcohol dehydrogenases with 2-ketoacid reductase activity. Exemplary secondary alcohol dehydrogenases include malate dehydrogenase, lactate dehydrogenase, 2-ketoadipate reductase, isopropanol dehydrogenase, methyl ethyl ketone reductase, and others described below and known in the art. Two secondary alcohol dehydrogenase enzymes from E. coli are encoded by malate dehydrogenase (mdh: EC 1.1.1.37, 1.1.1.82, 1.1.1.299) and lactate dehydrogenase (ldhA). S. cerevisiae encodes three copies of malate dehydrogenase, MDH1 (McAlister-Henn and Thompson, J. Bacteriol. 169:5157-5166 (1987), MDH2 (Minard and McAlister-Henn, Mol. Cell. Biol. 11:370-380 (1991); Gibson and McAlister-Henn, J. Biol. Chem. 278:25628-25636 (2003)), and MDH3 (Steffan and McAlister-Henn, J. Biol. Chem. 267:24708-24715 (1992)), which localize to the mitochondrion, cytosol, and peroxisome, respectively. Close homologs to the cytosolic malate dehydrogenase, MDH2, from S. cerevisiae are found in several organisms including Kluyveromyces lactis and Candida tropicalis. The lactate dehydrogenase from Ralstonia eutropha has been shown to demonstrate high activities on 2-ketoacids of various chain lengths includings lactate, 2-oxobutyrate, 2-oxopentanoate and 2-oxoglutarate (Steinbuchel et al., Eur. J. Biochem. 130:329-334 (1983)). Conversion of alpha-ketoadipate into alpha-hydroxyadipate can be catalyzed by 2-ketoadipate reductase, an enzyme reported to be found in rat and in human placenta (Suda et al., Arch. Biochem. Biophys. 176:610-620 (1976); Suda et al., Biochem. Biophys. Res. Commun. 77:586-591 (1977)). Alcohol dehydrogenase enzymes of C. beijerinckii (Ismaiel et al., J. Bacteriol. 175:5097-5105 (1993)) and T. brockii (Lamed et al., Biochem. J. 195:183-190 (1981); Peretz et al., Biochemistry. 28:6549-6555 (1989)) convert acetone to isopropanol. Methyl ethyl ketone reductase catalyzes the reduction of MEK to 2-butanol. Exemplary MEK reductase enzymes can be found in Rhodococcus ruber (Kosjek et al., Biotechnol Bioeng. 86:55-62 (2004)) and Pyrococcus furiosus (van der Oost et al., Eur. J. Biochem. 268:3062-3068 (2001)). The cloning of the bdhA gene from Rhizobium (Sinorhizobium) meliloti into E. coli conferred the ability to utilize 3-hydroxybutyrate as a carbon source (Aneja and Charles, J. Bacteriol. 181(3):849-857 (1999)). Additional candidates can be found in Pseudomonas fragi (Ito et al., J. Mol. Biol. 355(4) 722-733 (2006)) and Ralstonia pickettii (Takanashi et al., Antonie van Leeuwenoek, 95(3):249-262 (2009)). Recombinant 3-ketoacid reductase enzymes with broad substrate range and high activity have been characterized in US Application 2011/0201072, and are incorporated by reference herein. The mitochondrial 3-hydroxybutyrate dehydrogenase (bdh) from the human heart which has been cloned and characterized (Marks et al., J. Biol. Chem. 267:15459-15463 (1992)). Yet another secondary ADH, sadH of Candida parapsilosis, demonstrated activity on 3-oxobutanol (Matsuyama et al. J Mol Cat B Enz, 11:513-521 (2001)).
  • GenBank
    Gene Accession No. GI No. Organism
    mdh AAC76268.1 1789632 Escherichia coli
    ldhA NP_415898.1 16129341 Escherichia coli
    MDH1 NP_012838 6322765 Saccharomyces
    cerevisiae
    MDH2 NP_014515 116006499 Saccharomyces
    cerevisiae
    MDH3 NP_010205 6320125 Saccharomyces
    cerevisiae
    KLLA0E07525p XP_454288.1 50308571 Kluyveromyces
    lactis NRRL
    Y-1140
    YALI0D16753g XP_502909.1 50550873 Yarrowia
    lipolytica
    CTRG 01021 XP_002546239.1 255722609 Candida
    tropicalis
    MYA-3404
    ldh YP_725182.1 113866693 Ralstonia
    eutropha
    adh AAA23199.2 60592974 Clostridium
    beijerinckii
    NRRL B593
    adh P14941.1 113443 Therm-
    anaerobacter
    brockii HTD4
    sadh CAD36475 21615553 Rhodococcus ruber
    adhA AAC25556 3288810 Pyrococcus furiosus
    PRK13394 BAD86668.1 57506672 Pseudomonas fragi
    Bdh1 BAE72684.1 84570594 Ralstonia pickettii
    Bdh2 BAE72685.1 84570596 Ralstonia pickettii
    Bdh3 BAF91602.1 158937170 Ralstonia pickettii
    bdh AAA58352.1 177198 Homo sapiens
    sadh BAA24528.1 2815409 Candida
    parapsilosis
  • Alcohol dehydrogenases that reduce 3-ketoacids to their corresponding 3-hydroxyacids, required for the reduction of 3-oxo-4-hydroxypentanoate (3C), have also been characterized. These enzymes include 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30), 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31), threonine dehydrogenase (EC 1.1.1.103), 3-hydroxypropionate dehydrogenase (EC 1.1.1.298) and benzyl-2-methyl-3-hydroxybutanoate dehydrogenase (EC 1.1.1.217). Recombinant 3-ketoacid reductase enzymes with broad substrate range and high activity have been characterized in US Application 2011/0201072, and are incorporated by reference herein. The mitochondrial 3-hydroxybutyrate dehydrogenase (bdh) from the human heart which has been cloned and characterized (Marks et al., J. Biol. Chem. 267:15459-15463 (1992)). Secondary alcohol dehydrogenases described above are also suitable here.
  • Gene GenBank Accession No. GI No. Organism
    Bdh AAA58352.1 177198 Homo sapiens
  • Alcohol dehydrogenase enzymes active on allyl alcohols are suitable for reducing crotyl aldehyde to crotyl alcohol (1J). Crotyl aldehyde reductase activity has been demonstrated by mdr of Synechocystis sp. PCC 6803 (Shimakawa et al, Biosci Biotechnol Biochem 77:2441-8 (2013)). An exemplary allyl alcohol dehydrogenase is the NtRed-1 enzyme from Nicotiana tabacum (Matsushima et al, Bioorg Chem 36: 23-8 (2008)). A similar enzyme has been characterized in Pseudomonas putida MB1 but the enzyme has not been associated with a gene to date (Malone et al, AEM 65: 2622-30 (1999)). Yet another allyl alcohol dehydrogenase is the geraniol dehydrogenase enzymes of Castellaniella defragrans, Carpoglyphus lactis and Ocimum basilicum (Lueddeke et al, AEM 78:2128-36 (2012)). Alcohol dehydrogenase enzymes with broad substrate specificity are also applicable here, such as include alrA encoding a medium-chain alcohol dehydrogenase for C2-C14 (Tani et al., Appl. Environ. Microbiol. 66:5231-5235 (2000)), yqhD, yahK, adhE and fucO from E. coli (Sulzenbacher et al., J Mol Biol 342:489-502 (2004)), and bdh I and bdh II from C. acetobutylicum which converts butyryaldehyde into butanol (Walter et al, J. Bacteriol 174:7149-7158 (1992)). YqhD of E. coli catalyzes the reduction of a wide range of aldehydes using NADPH as the cofactor, with a preference for chain lengths longer than C(3) (Sulzenbacher et al, J Mol Biol 342:489-502 (2004); Perez et al., J Biol. Chem. 283:7346-7353 (2008)). The adhA gene product from Zymomonas mobilis has been demonstrated to have activity on a number of aldehydes including formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde, and acrolein (Kinoshita et al., Appl Microbiol Biotechnol 22:249-254 (1985)).
  • GenBank
    Gene Accession No. GI No. Organism
    MDR BAM52497.1 407959257 Synechocystis sp. PCC 6803
    NT-RED1 BAA89423 6692816 Nicotiana tabacum
    geoA CCF55024.1 372099287 Castellaniella defragrans
    GEDH1 Q2KNL6.1 122200955 Ocimum basilicum
    GEDH BAG32342.1 188219500 Carpoglyphus lactis
    alrA BAB12273.1 9967138 Acinetobacter sp. strain M-1
    ADH2 NP_014032.1 6323961 Saccharomyces cerevisiae
    fucO NP_417279.1 16130706 Escherichia coli
    yqhD NP_417484.1 16130909 Escherichia coli
    yahK P75691.1 2492774 Escherichia coli
    adhE NP_415757.1 16129202 Escherichia coli
    bdh I NP_349892.1 15896543 Clostridium acetobutylicum
    bdh II NP_349891.1 15896542 Clostridium acetobutylicum
    adhA YP_162971.1 56552132 Zymomonas mobilis
    bdh BAF45463.1 124221917 Clostridium
    saccharoperbutylacetonicum
  • Alcohol dehydrogenases active on 3-hydroxyacyl-CoA and 2-hydroxyacyl-CoA substrates catalyze the reduction of 2-oxopent-3-enoyl-CoA (FIG. 1O) and 3-oxo-4-hydroxypentanoyl-CoA (FIG. 3G) to their corresponding hydroxyacyl-CoA products. 3-Oxoacyl-CoA reductase enzymes (EC 1.1.1.35) convert 3-oxoacyl-CoA molecules into 3-hydroxyacyl-CoA molecules and are often involved in fatty acid beta-oxidation or phenylacetate catabolism. For example, subunits of two fatty acid oxidation complexes in E. coli, encoded by fadB and fadJ, function as 3-hydroxyacyl-CoA dehydrogenases (Binstock et al., Methods Enzymol. 71 Pt C:403-411 (1981)). The paaH gene product has a similar activity (Nogales et al., 153:357-365 (2007)). Additional 3-oxoacyl-CoA enzymes include the gene products of phaC in Pseudomonas putida (Olivera et al., Proc. Natl. Acad. Sci. U.S.A 95:6419-6424 (1998)) and paaC in Pseudomonas fluorescens (Di et al., 188:117-125 (2007)). These enzymes catalyze the reversible oxidation of 3-hydroxyadipyl-CoA to 3-oxoadipyl-CoA during the catabolism of phenylacetate or styrene. Acetyoacetyl-CoA reductase enzymes include hbd of Clostridium acetobutylicum (Youngleson et al., J Bacteriol. 171:6800-6807 (1989)), phbB from Zoogloea ramigera (Ploux et al., Eur. J Biochem. 174:177-182 (1988)) and phaB from Rhodobacter sphaeroides (Alber et al., Mol. Microbiol 61:297-309 (2006)). The former gene is NADPH-dependent, its nucleotide sequence has been determined (Peoples et al., Mol. Microbiol 3:349-357 (1989)) and the gene has been expressed in E. coli. Substrate specificity studies on the gene led to the conclusion that it could accept 3-oxopropionyl-CoA as a substrate besides acetoacetyl-CoA (Ploux et al., Eur. J Biochem. 174:177-182 (1988)). 3-Hydroxyacyl-CoA dehydrogenases that accept longer acyl-CoA substrates (eg. EC 1.1.1.35) are typically involved in beta-oxidation. An example is HSD17B10 in Bos taurus (WAKIL et al., J Biol. Chem. 207:631-638 (1954)). An exemplary 2-oxoacyl-CoA reductase is the 3-hydroxy-2-methylbutyryl-CoA dehydrogenase (EC 1.1.1.178) of Pseudomonas putida, which catalyzes the reduction of 3-methyl-2-oxopentanoyl-CoA, in addition to its native activity (Conrad et al, J Bacteriol 118:103-11 (1974)).
  • Protein GENBANK ID GI NUMBER ORGANISM
    fadB P21177.2 119811 Escherichia coli
    fadJ P77399.1 3334437 Escherichia coli
    paaH NP_415913.1 16129356 Escherichia coli
    Hbd2 EDK34807.1 146348271 Clostridium kluyveri
    Hbd1 EDK32512.1 146345976 Clostridium kluyveri
    phaC NP_745425.1 26990000 Pseudomonas putida
    paaC ABF82235.1 106636095 Pseudomonas fluorescens
    HSD17B10 O02691.3 3183024 Bos taurus
    phbB P23238.1 130017 Zoogloea ramigera
    phaB YP_353825.1 77464321 Rhodobacter sphaeroides
    phaB BAA08358 675524 Paracoccus denitrificans
    Hbd NP_349314.1 15895965 Clostridium
    acetobutylicum
    Hbd AAM14586.1 20162442 Clostridium beijerinckii
    HSD17B10 O02691.3 3183024 Bos taurus
    • 1.2.1.b Acyl-CoA Reductase (Aldehyde Forming)
  • Acetyl-CoA reductase (an acyl-CoA reductase in EC class 1.2.1.-) catalyzes the reduction of acetyl-CoA to acetaldehyde. Several acyl-CoA dehydrogenases reduce an acyl-CoA to its corresponding aldehyde and represent suitable enzyme candidates for catalyzing step A of FIGS. 1 and 2. The NAD(P)H dependent reduction of acetyl-CoA to acetaldehyde is catalyzed by acylating acetaldehyde dehydrogenase (EC 1.2.1.10). Acylating acetaldehyde dehydrogenase enzymes of E. coli are encoded by adhE and mhpF (Ferrandez et al, J Bacteriol 179:2573-81 (1997)). The Pseudomonas sp. CF600 enzyme, encoded by dmpF, participates in meta-cleavage pathways and forms a complex with 4-hydroxy-2-oxovalerate aldolase (Shingler et al, J Bacteriol 174:711-24 (1992)). Solventogenic organisms such as Clostridium acetobutylicum encode bifunctional enzymes with alcohol dehydrogenase and acetaldehyde dehydrogenase activities. The bifunctional C. acetobutylicum enzymes are encoded by bdh I and adhE2 (Walter, et al., J. Bacteriol. 174:7149-7158 (1992); Fontaine et al., J. Bacteriol. 184:821-830 (2002)). Yet another candidate for acylating acetaldehyde dehydrogenase is the ald gene from Clostridium beijerinckii (Toth, Appl. Environ. Microbiol. 65:4973-4980 (1999). This gene is very similar to the eutE acetaldehyde dehydrogenase genes of Salmonella typhimurium and E. coli (Toth, Appl. Environ. Microbiol. 65:4973-4980 (1999). Other exemplary enzymes with acetyl-CoA reductase activity are found in the EC 1.2.1.-enzyme class including fatty acyl-CoA reductase, succinyl-CoA reductase (EC 1.2.1.76), acetyl-CoA reductase, butyryl-CoA reductase and propionyl-CoA reductase (EC 1.2.1.3). Such enzymes include bphG of Pseudomonas sp (Powlowski, J. Bacteriol. 175:377-385 (1993)), adhE in Leuconostoc mesenteroides and Escherichia coli (Kazahaya, J. Gen. Appl. Microbiol. 18:43-55 (1972); and Koo et al., Biotechnol Lett. 27:505-510 (2005)) and butyraldehyde dehydrogenase enzymes of solventogenic organisms such as Clostridium saccharoperbutylacetonicum (Kosaka et al., Biosci Biotechnol Biochem., 71:58-68 (2007)). Enzymes outside the EC class 1.2.1. which convert acetyl-CoA to acetaldehyde include bifunctional dehydrogenase/aldolases which degrade 4-hydroxy-2-oxovalerate to pyruvate and acetyl-CoA.
  • Protein GenBank ID GI Number Organism
    adhE NP_415757.1 16129202 Escherichia coli
    mhpF NP_414885.1 16128336 Escherichia coli
    dmpF CAA43226.1 45683 Pseudomonas sp. CF600
    adhE2 AAK09379.1 12958626 Clostridium acetobutylicum
    bdh I NP_349892.1 15896543 Clostridium acetobutylicum
    Ald AAT66436 49473535 Clostridium beijerinckii
    eutE NP_416950 16130380 Escherichia coli
    eutE AAA80209 687645 Salmonella typhimurium
    bphG BAA03892.1 425213 Pseudomonas sp
    adhE AAV66076.1 55818563 Leuconostoc mesenteroides
    bld AAP42563.1 31075383 Clostridium
    saccharoperbutylacetonicum
    dmpG CAA43227.1 45684 Pseudomonas sp. CF600
    dmpF CAA43226.1 45683 Pseudomonas sp. CF600
    bphI ABE37049.1 91693852 Burkholderia xenovorans
    bphJ ABE37050.1 91693853 Burkholderia xenovorans
    • 2.3.1.b Beta-Ketothiolase
  • Beta-ketothiolase enzymes are required for the conversion of lactoyl-CoA and acetyl-CoA to 3-oxo-4-hydroxypentanoyl-CoA, shown in FIG. 3A. Suitable enzymes are found in EC class 2.3.1, and include beta-ketovaleryl-CoA thiolase, acetoacetyl-CoA thiolase and beta-ketoadipyl-CoA thiolase. Beta-ketovaleryl-CoA thiolase catalyzes the formation of beta-ketovalerate from acetyl-CoA and propionyl-CoA. Zoogloea ramigera possesses two ketothiolases that can form beta-ketovaleryl-CoA from propionyl-CoA and acetyl-CoA and R. eutropha has a beta-oxidation ketothiolase that is also capable of catalyzing this transformation (Gruys et al., U.S. Pat. No. 5,958,745). The sequences of these genes or their translated proteins have not been reported, but several genes in R. eutropha, Z. ramigera, or other organisms can be identified based on sequence homology to bktB from R. eutropha. Acetoacetyl-CoA thiolase converts two molecules of acetyl-CoA into acetoacetyl-CoA (EC 2.1.3.9). Exemplary acetoacetyl-CoA thiolase enzymes include the gene products of atoB from E. coli (Martin et al., Nat. Biotechnol. 21:796-802 (2003)), thlA and thlB from C. acetobutylicum (Hanai et al., Appl. Environ. Microbiol. 73:7814-7818 (2007); Winzer et al., J. Mol. Microbiol. Biotechnol. 2:531-541 (2000)), and ERG10 from S. cerevisiae (Hiser et al., J. Biol. Chem. 269:31383-31389 (1994)). Beta-ketoadipyl-CoA thiolase (EC 2.3.1.174), also called 3-oxoadipyl-CoA thiolase, converts beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA, and is a key enzyme of the beta-ketoadipate pathway for aromatic compound degradation. The enzyme is widespread in soil bacteria and fungi including Pseudomonas putida (Harwood et al., J. Bacteriol. 176-6479-6488 (1994)) and Acinetobacter calcoaceticus (Doten et al., J. Bacteriol. 169:3168-3174 (1987)). The P. putida enzyme is a homotetramer bearing 45% sequence homology to beta-ketothiolases involved in PHB synthesis in Ralstonia eutropha, fatty acid degradation by human mitochondria and butyrate production by Clostridium acetobutylicum (Harwood et al., supra). A beta-ketoadipyl-CoA thiolase in Pseudomonas knackmussii (formerly sp. B13) has also been characterized (Gobel et al., J. Bacteriol. 184:216-223 (2002); Kaschabek et al., supra). BKT encoding genes and associated identifiers are shown in the table below.
  • Protein GenBank ID GI Number Organism
    phaA YP_725941.1 113867452 Ralstonia eutropha
    h16_A1713 YP_726205.1 113867716 Ralstonia eutropha
    pcaF YP_728366.1 116694155 Ralstonia eutropha
    h16_B1369 YP_840888.1 116695312 Ralstonia eutropha
    h16_A0170 YP_724690.1 113866201 Ralstonia eutropha
    h16_A0462 YP_724980.1 113866491 Ralstonia eutropha
    h16_A1528 YP_726028.1 113867539 Ralstonia eutropha
    h16_B0381 YP_728545.1 116694334 Ralstonia eutropha
    h16_B0662 YP_728824.1 116694613 Ralstonia eutropha
    h16_B0759 YP_728921.1 116694710 Ralstonia eutropha
    h16_B0668 YP_728830.1 116694619 Ralstonia eutropha
    h16_A1720 YP_726212.1 113867723 Ralstonia eutropha
    h16_A1887 YP_726356.1 113867867 Ralstonia eutropha
    phbA P07097.4 135759 Zoogloea ramigera
    bktB YP_002005382.1 194289475 Cupriavidus taiwanensis
    Rmet_1362 YP_583514.1 94310304 Ralstonia metallidurans
    Bphy_0975 YP_001857210.1 186475740 Burkholderia phymatum
    atoB NP_416728 16130161 Escherichia coli
    thlA NP_349476.1 15896127 Clostridium acetobutylicum
    thlB NP_149242.1 15004782 Clostridium acetobutylicum
    ERG10 NP_015297 6325229 Saccharomyces cerevisiae
    pcaF NP_743536.1 506695 Pseudomonas putida
    pcaF AAC37148.1 141777 Acinetobacter calcoaceticus
    catF Q8VPF1.1 75404581 Pseudomonas knackmussii
    • 2.7.1.a Kinase
  • CrotOH kinase enzymes catalyze the transfer of a phosphate group to the hydroxyl group of CrotOH, shown in step A of FIG. 4. The enzymes described below naturally possess such activity or can be engineered to exhibit this activity. Kinases that catalyze transfer of a phosphate group to an alcohol group are members of the EC 2.7.1 enzyme class. The table below lists several useful kinase enzymes in the EC 2.7.1 enzyme class.
  • Enzyme
    Commission
    Number Enzyme Name
    2.7.1.1 hexokinase
    2.7.1.2 glucokinase
    2.7.1.3 ketohexokinase
    2.7.1.4 fructokinase
    2.7.1.5 rhamnulokinase
    2.7.1.6 Galactokinase
    2.7.1.7 Mannokinase
    2.7.1.8 glucosamine kinase
    2.7.1.10 phosphoglucokinase
    2.7.1.11 6-phosphofructokinase
    2.7.1.12 gluconokinase
    2.7.1.13 dehydrogluconokinase
    2.7.1.14 sedoheptulokinase
    2.7.1.15 ribokinase
    2.7.1.16 ribulokinase
    2.7.1.17 xylulokinase
    2.7.1.18 phosphoribokinase
    2.7.1.19 phosphoribulokinase
    2.7.1.20 adenosine kinase
    2.7.1.21 thymidine kinase
    2.7.1.22 ribosylnicotinamide kinase
    2.7.1.23 NAD+ kinase
    2.7.1.24 dephospho-CoA kinase
    2.7.1.25 adenylyl-sulfate kinase
    2.7.1.26 riboflavin kinase
    2.7.1.27 erythritol kinase
    2.7.1.28 triokinase
    2.7.1.29 glycerone kinase
    2.7.1.30 glycerol kinase
    2.7.1.31 glycerate kinase
    2.7.1.32 choline kinase
    2.7.1.33 pantothenate kinase
    2.7.1.34 pantetheine kinase
    2.7.1.35 pyridoxal kinase
    2.7.1.36 mevalonate kinase
    2.7.1.39 homoserine kinase
    2.7.1.40 pyruvate kinase
    2.7.1.41 glucose-1-phosphate phosphodismutase
    2.7.1.42 riboflavin phosphotransferase
    2.7.1.43 glucuronokinase
    2.7.1.44 galacturonokinase
    2.7.1.45 2-dehydro-3-deoxygluconokinase
    2.7.1.46 L-arabinokinase
    2.7.1.47 D-ribulokinase
    2.7.1.48 uridine kinase
    2.7.1.49 hydroxymethylpyrimidine kinase
    2.7.1.50 hydroxyethylthiazole kinase
    2.7.1.51 L-fuculokinase
    2.7.1.52 fucokinase
    2.7.1.53 L-xylulokinase
    2.7.1.54 D-arabinokinase
    2.7.1.55 allose kinase
    2.7.1.56 1-phosphofructokinase
    2.7.1.58 2-dehydro-3-deoxy galactonokinase
    2.7.1.59 N-acetylglucosamine kinase
    2.7.1.60 N-acylmannosamine kinase
    2.7.1.61 acyl-phosphate-hexose phosphotransferase
    2.7.1.62 phosphoramidate-hexose phosphotransferase
    2.7.1.63 polyphosphate-glucose phosphotransferase
    2.7.1.64 inositol 3-kinase
    2.7.1.65 scyllo-inosamine 4-kinase
    2.7.1.66 undecaprenol kinase
    2.7.1.67 1-phosphatidylinositol 4-kinase
    2.7.1.68 1-phosphatidylinositol-4-phosphate 5-kinase
    2.7.1.69 protein-Np-phosphohistidine-sugar phosphotransferase
    2.7.1.70 identical to EC 2.7.1.37.
    2.7.1.71 shikimate kinase
    2.7.1.72 streptomycin 6-kinase
    2.7.1.73 inosine kinase
    2.7.1.74 deoxycytidine kinase
    2.7.1.76 deoxyadenosine kinase
    2.7.1.77 nucleoside phosphotransferase
    2.7.1.78 polynucleotide 5′-hydroxyl-kinase
    2.7.1.79 diphosphate-glycerol phosphotransferase
    2.7.1.80 diphosphate-serine phosphotransferase
    2.7.1.81 hydroxylysine kinase
    2.7.1.82 ethanolamine kinase
    2.7.1.83 pseudouridine kinase
    2.7.1.84 alkylglycerone kinase
    2.7.1.85 β-glucoside kinase
    2.7.1.86 NADH kinase
    2.7.1.87 streptomycin 3″-kinase
    2.7.1.88 dihydrostreptomycin-6-phosphate 3′a-kinase
    2.7.1.89 thiamine kinase
    2.7.1.90 diphosphate-fructose-6-phosphate phosphotransferase
    2.7.1.91 sphinganine kinase
    2.7.1.92 5-dehydro-2-deoxygluconokinase
    2.7.1.93 alkylglycerol kinase
    2.7.1.94 acylglycerol kinase
    2.7.1.95 kanamycin kinase
    2.7.1.100 S-methyl-5-thioribose kinase
    2.7.1.101 tagatose kinase
    2.7.1.102 hamamelose kinase
    2.7.1.103 viomycin kinase
    2.7.1.105 6-phosphofructo-2-kinase
    2.7.1.106 glucose-1,6-bisphosphate synthase
    2.7.1.107 diacylglycerol kinase
    2.7.1.108 dolichol kinase
    2.7.1.113 deoxyguanosine kinase
    2.7.1.114 AMP-thymidine kinase
    2.7.1.118 ADP-thymidine kinase
    2.7.1.119 hygromycin-B 7″-O-kinase
    2.7.1.121 phosphoenolpyruyate-glycerone phosphotransferase
    2.7.1.122 xylitol kinase
    2.7.1.127 inositol-trisphosphate 3-kinase
    2.7.1.130 tetraacyldisaccharide 4′-kinase
    2.7.1.134 inositol-tetrakisphosphate 1-kinase
    2.7.1.136 macrolide 2′-kinase
    2.7.1.137 phosphatidylinositol 3-kinase
    2.7.1.138 ceramide kinase
    2.7.1.140 inositol-tetrakisphosphate 5-kinase
    2.7.1.142 glycerol-3-phosphate-glucose phosphotransferase
    2.7.1.143 diphosphate-purine nucleoside kinase
    2.7.1.144 tagatose-6-phosphate kinase
    2.7.1.145 deoxynucleoside kinase
    2.7.1.146 ADP-dependent phosphofructokinase
    2.7.1.147 ADP-dependent glucokinase
    2.7.1.148 4-(cytidine 5′-diphospho)-2-C-methyl-D-erythritol kinase
    2.7.1.149 1-phosphatidylinositol-5-phosphate 4-kinase
    2.7.1.150 1-phosphatidylinositol-3-phosphate 5-kinase
    2.7.1.151 inositol-polyphosphate multikinase
    2.7.1.153 phosphatidylinositol-4,5-bisphosphate 3-kinase
    2.7.1.154 phosphatidylinositol-4-phosphate 3-kinase
    2.7.1.156 adenosylcobinamide kinase
    2.7.1.157 N-acetylgalactosamine kinase
    2.7.1.158 inositol-pentakisphosphate 2-kinase
    2.7.1.159 inositol-1,3,4-trisphosphate 5/6-kinase
    2.7.1.160 2′-phosphotransferase
    2.7.1.161 CTP-dependent riboflavin kinase
    2.7.1.162 N-acetylhexosamine 1-kinase
    2.7.1.163 hygromycin B 4-O-kinase
    2.7.1.164 O-phosphoseryl-tRNASec kinase
  • Mevalonate kinase (EC 2.7.1.36) phosphorylates the terminal hydroxyl group of mevalonate. Gene candidates for this step include erg12 from S. cerevisiae, mvk from Methanocaldococcus jannaschi, MVK from Homo sapeins, and mvk from Arabidopsis thaliana col. Additional mevalonate kinase candidates include the feedback-resistant mevalonate kinase from the archeon Methanosarcina mazei (Primak et al, AEM, in press (2011)) and the Mvk protein from Streptococcus pneumoniae (Andreassi et al, Protein Sci, 16:983-9 (2007)). Mvk proteins from S. cerevisiae, S. pneumoniae and M. mazei were heterologously expressed and characterized in E. coli (Primak et al, supra). The S. pneumoniae mevalonate kinase was active on several alternate substrates including cylopropylmevalonate, vinylmevalonate and ethynylmevalonate (Kudoh et al, Bioorg Med Chem 18:1124-34 (2010)), and a subsequent study determined that the ligand binding site is selective for compact, electron-rich C(3)-substituents (Lefurgy et al, J Biol Chem 285:20654-63 (2010)).
  • Protein GenBank ID GI Number Organism
    erg12 CAA39359.1 3684 Sachharomyces cerevisiae
    mvk Q58487.1 2497517 Methanocaldococcus jannaschii
    mvk AAH16140.1 16359371 Homo sapiens
    mvk NP_851084.1 30690651 Arabidopsis thaliana
    mvk NP_633786.1 21227864 Methanosarcina mazei
    mvk NP_357932.1 15902382 Streptococcus pneumoniae
  • Glycerol kinase also phosphorylates the terminal hydroxyl group in glycerol to form glycerol-3-phosphate. This reaction occurs in several species, including Escherichia coli, Saccharomyces cerevisiae, and Thermotoga maritima. The E. coli glycerol kinase has been shown to accept alternate substrates such as dihydroxyacetone and glyceraldehyde (Hayashi et al., J Biol. Chem. 242:1030-1035 (1967)). T, maritime has two glycerol kinases (Nelson et al., Nature 399:323-329 (1999)). Glycerol kinases have been shown to have a wide range of substrate specificity. Crans and Whiteside studied glycerol kinases from four different organisms (Escherichia coli, S. cerevisiae, Bacillus stearothermophilus, and Candida mycoderma) (Crans et al., J. Am. Chem. Soc. 107:7008-7018 (2010); Nelson et al., supra, (1999)). They studied 66 different analogs of glycerol and concluded that the enzyme could accept a range of substituents in place of one terminal hydroxyl group and that the hydrogen atom at C2 could be replaced by a methyl group. Interestingly, the kinetic constants of the enzyme from all four organisms were very similar.
  • Protein GenBank ID GI Number Organism
    glpK AP_003883.1 89110103 Escherichia coli K12
    glpK1 NP_228760.1 15642775 Thermotoga maritime MSB8
    glpK2 NP_229230.1 15642775 Thermotoga maritime MSB8
    Gut1 NP_011831.1 82795252 Saccharomyces cerevisiae
  • Homoserine kinase is another possible candidate. This enzyme is also present in a number of organisms including E. coli, Streptomyces sp, and S. cerevisiae. Homoserine kinase from E. coli has been shown to have activity on numerous substrates, including, L-2-amino,1,4-butanediol, aspartate semialdehyde, and 2-amino-5-hydroxyvalerate (Huo et al., Biochemistry 35:16180-16185 (1996); Huo et al., Arch. Biochem. Biophys. 330:373-379 (1996)). This enzyme can act on substrates where the carboxyl group at the alpha position has been replaced by an ester or by a hydroxymethyl group.
  • Protein GenBank ID GI Number Organism
    thrB BAB96580.2 85674277 Escherichia
    coli K12
    SACT1DRAFT_4809 ZP_06280784.1 282871792 Streptomyces
    sp. ACT-1
    Thr1 AAA35154.1 172978 Saccharomyces
    serevisiae
  • Other classes of kinases that can catalyze the phosphorylation of crotyl alcohol are hydroxyethylthiazole kinase (FIG. 8 is a graph of in vitro formation of crotyl phosphate (CrPi) and ADP over time from a composition including ATP, crotyl alcohol, and hydroxyethylthiazole kinase), thiamine kinase, pantothenate kinase, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, riboflavin kinase, L-fuculokinase and choline kinase. Exemplary gene candidates for each of these classes are shown below.
  • The table below provides gene candidates for hydroxyethyl thiazole kinases
  • Protein GenBank ID GI Number Organism
    ThiM YP_007535827.1 16080881 Bacillus subtilis
    Thi6 CAA97929.1 1370444 Saccharomyces serevisiae
    ThiM NP_372616.1 15925082 Staphylococcus aureus
    PH1157, thiM NP_143059.1 14590984 Pyrococcus
    (analogue of horikoshii OT3
    thiK)
    ThiM Q830K4 81585041 Enterococcus faecalis
    V583
    ThiM YP_006701495 405760899 Streptococcus pneumoniae
    SPNA45
    ThiM YP_004888181 380031190 Lactobacillus plantarum
    WCFS1
    ThiM WP_012906431 502670591 Citrobacter rodentium
    ThiM NP_461091 16765476 Salmonella enterica
    subsp. Enterica LT2
    ThiM YP_771477 116255644 Rhizobium
    leguminosarum bv.
    viciae 3841
    ThiM (b2104) AAC75165.1 1788421 Escherichia coli str. K-
    12 substr. MG1655
  • Some candidate thiamine kinases are:
  • Protein GenBank ID GI Number Organism
    thiK AAC74190.1 1787349 Escherichia coli K12
    thiK NP_460178.1 16764563 Salmonella enterica subsp.
    enterica serovar
    Typhimurium str. LT2
  • Examplary fuculokinases are:
  • Protein GenBank ID GI Number Organism
    b2803 AAC75845.1  1789168 Escherichia coli K12
    MG1655
    STM14_3591 ACY90002.1 267995117 Salmonella enterica subsp.
    enterica serovar
    Typhimurium str.
    14028S
    D186_16909 EKS55716.1 411772069 Citrobacter freundii ATCC
    8090 = MTCC 1658
  • 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase can also carry the described transformation. Gene candidates from this class are listed below.
  • Protein GenBank ID GI Number Organism
    folK AAC73253.1  1786335 Escherichia coli K12
    folK NP_816865.1 29377711 Enterococcus faecalis V583
  • Pantothenate kinases that can catalyze the transformation are:
  • Protein GenBank ID GI Number Organism
    CoaA YP_006514461.1 397672926 Mycobacterium
    tuberculosis H37Rv
    CoaA YP_491482.1 388479290 Escherichia coli K12
    CoaX Q9WZY5.1  81553296 Thermotoga maritima
    MSB8
    Sav2130 NP_372654.1  15925120 Staphylococcus aureus
    subsp. aureus Mu50
  • Yet another candidate enzyme class of interest is 4-(cytidine 5′-diphospho)-2-C-methyl-D-erythritol kinase (2.7.1.148). Gene candidates from this class are:
  • Protein GenBank ID GI Number Organism
    ispE NP_415726.1  16129171 Escherichia coli K12
    ispE KBJ36713.1 623367758 Mycobacterium tuberculosis
    H37Rv
    • 2.7.4.a Phosphokinase
  • 2-Butenyl-4-phosphate kinase enzymes catalyze the transfer of a phosphate group to the phosphate group of 2-butenyl-4-phosphate (FIG. 4B). The enzymes described below naturally possess such activity or can be engineered to exhibit this activity. Kinases that catalyze transfer of a phosphate group to another phosphate group are members of the EC 2.7.4 enzyme class. The table below lists several useful kinase enzymes in the EC 2.7.4 enzyme class.
  • Enzyme
    Commission
    Number Enzyme Name
    2.7.4.1 polyphosphate kinase
    2.7.4.2 phosphomevalonate kinase
    2.7.4.3 adenylate kinase
    2.7.4.4 nucleoside-phosphate kinase
    2.7.4.6 nucleoside-diphosphate kinase
    2.7.4.7 phosphomethylpyrimidine kinase
    2.7.4.8 guanylate kinase
    2.7.4.9 dTMP kinase
    2.7.4.10 nucleoside-triphosphate—adenylate kinase
    2.7.4.11 (deoxy)adenylate kinase
    2.7.4.12 T2-induced deoxynucleotide kinase
    2.7.4.13 (deoxy)nucleoside-phosphate kinase
    2.7.4.14 cytidylate kinase
    2.7.4.15 thiamine-diphosphate kinase
    2.7.4.16 thiamine-phosphate kinase
    2.7.4.17 3-phosphoglyceroyl-phosphate—polyphosphate
    phosphotransferase
    2.7.4.18 famesyl-diphosphate kinase
    2.7.4.19 5-methyldeoxycytidine-5′-phosphate kinase
    2.7.4.20 dolichyl-diphosphate—polyphosphate
    phosphotransferase
    2.7.4.21 inositol-hexakisphosphate kinase
    2.7.4.22 UMP kinase
    2.7.4.23 ribose 1,5-bisphosphate phosphokinase
    2.7.4.24 diphosphoinositol-pentakisphosphate kinase
    2.7.4.— Famesyl monophosphate kinase
    2.7.4.— Geranyl-geranyl monophosphate kinase
    2.7.4.— Phytyl-phosphate kinase
    2.7.4.26 isopentenyl phosphate kinase
  • Phosphomevalonate kinase enzymes are of particular interest. Phosphomevalonate kinase (EC 2.7.4.2) catalyzes the analogous transformation to 2-butenyl-4-phosphate kinase. This enzyme is encoded by erg8 in Saccharomyces cerevisiae (Tsay et al., Mol. Cell Biol. 11:620-631 (1991)) and mvaK2 in Streptococcus pneumoniae, Staphylococcus aureus and Enterococcus faecalis (Doun et al., Protein Sci. 14:1134-1139 (2005); Wilding et al., J Bacteriol. 182:4319-4327 (2000)). The Streptococcus pneumoniae and Enterococcus faecalis enzymes were cloned and characterized in E. coli (Pilloff et al., J Biol. Chem. 278:4510-4515 (2003); Doun et al., Protein Sci. 14:1134-1139 (2005)). The S. pneumoniae phosphomevalonate kinase was active on several alternate substrates including cylopropylmevalonate phosphate, vinylmevalonate phosphate and ethynylmevalonate phosphate (Kudoh et al, Bioorg Med Chem 18:1124-34 (2010)). These and related enzymes are shown in the table below.
  • GI
    Enzyme Genbank ID Number Organism
    Erg8 AAA34596.1 171479 Saccharomyces cerevisiae
    mvaK2 AAG02426.1 9937366 Staphylococcus aureus
    mvaK2 AAG02457.1 9937409 Streptococcus pneumoniae
    mvaK2 AAG02442.1 9937388 Enterococcus faecalis
    phosphomevalonate YP_008718968.1 554649894 Carnobacterium sp. WN1359
    kinase
    phosphomevalonate YP_004889541.1 380032550 Lactobacillus plantarum WCFS1
    kinase
    phosphomevalonate BAD86802.1 57753872 Streptomyces sp. KO-3988
    kinase
    phosphomevalonate YP_006806525.1 407642766 Nocardia brasiliensis ATCC
    kinase 700358
    phosphomevalonate YP_008165221.1 521188403 Corynebacterium terpenotabidum
    kinase Y-11
    isopentenyl phosphate NP_247007.1 15668214 Methanocaldococcus jannaschii
    kinase
    isopentenyl phosphate NP_393581.1 16081271 Thermoplasma acidophilum DSM
    kinase 1728
    isopentenyl phosphate NP_275190.1 15678076 Methanothermobacter
    kinase thermautotrophicus
    isopentenyl phosphate YP_003356693.1 282164308 Methanocella paludicola SANAE
    kinase
    isopentenyl phosphate YP_304959.1 73668944 Methanosarcina barkeri Fusaro
    kinase
    isopentenyl phosphate YP_007714098.1 478483448 Candidatus Methanomethylophilus-
    kinase alvus Mx1201
    isopentenyl phosphate AAB84554.1 2621082 Methanobacterium
    kinase thermoautotrophicum
    Isopentenyl phosphate D4GWT7.1 635552533 Haloferax volcanii
    kinase (JFK)
  • Additional kinase enzymes include fosfomycin kinase (FomA) which is highly homologous to isopentenyl phosphate kinase and is an antibiotic resistance enzyme found in a few strains of Streptomyces and Pseudomonas (Mabangalo et al. Biochemistry 51(4):917-925 (2012)). Superposition of Thermoplasma acidophilum (THA) IPK and FomA structures aligns their respective substrates and catalytic residues. These residues are conserved only in the IPK and FomA members of the phosphate subdivision of the amino acid kinase superfamily. IPK from Thermoplasma acidophilum has been shown to have activity on fosmomycin. An exemplary fosfomycin kinase is that from Streptomyces wedmorensis, Genbank ID BAA32493.1 and GI number 3452580.
  • Farnesyl monophosphate kinase enzymes catalyze the CTP dependent phosphorylation of farnesyl monophosphate to farnesyl diphosphate. Similarly, geranylgeranyl phosphate kinase catalyzes CTP dependent phosphorylation. Enzymes with these activities were identified in the microsomal fraction of cultured Nicotiana tabacum (Thai et al, PNAS 96:13080-5 (1999)). However, the associated genes have not been identified to date.
  • Additional enzymes include those of the EC 2.7.2.8 class. This class is exemplified by acetylglutamate kinase, including the exemplary enzymes below:
  •
    acetylglutamate NP_126233.1 14520758 Pyrococcus abyssi GE5
    kinase
    acetylglutamate NP_579365.1 18978008 Pyrococcus furiosus
    kinase DSM 3638
    acetylglutamate AAB88966.1  2648231 Archaeoglobus fulgidus
    kinase DSM4304
    • 2.7.6.a Diphosphokinase
  • CrotOH diphosphokinase enzymes catalyze the transfer of a diphosphate group to the hydroxyl group of CrotOH, shown in step I of FIG. 1. The enzymes described below naturally possess such activity or can be engineered to exhibit this activity. Kinases that catalyze transfer of a diphosphate group are members of the EC 2.7.6 enzyme class. The table below lists several useful kinase enzymes in the EC 2.7.6 enzyme class.
  • Enzyme
    Commission Number Enzyme Name
    2.7.6.1 ribose-phosphate diphosphokinase
    2.7.6.2 thiamine diphosphokinase
    2.7.6.3 2-amino-4-hydroxy-6-
    hydroxymethyldihydropteridine
    diphosphokinase
    2.7.6.4 nucleotide diphosphokinase
    2.7.6.5 GTP diphosphokinase
  • Of particular interest are ribose-phosphate diphosphokinase enzymes which have been identified in Escherichia coli (Hove-Jenson et al., J Biol Chem, 1986, 261(15); 6765-71) and Mycoplasma pneumoniae M129 (McElwain et al, International Journal of Systematic Bacteriology, 1988, 38:417-423) as well as thiamine diphosphokinase enzymes. Exemplary thiamine diphosphokinase enzymes are found in Arabidopsis thaliana (Ajjawi, Plant Mol Biol, 2007, 65(1-2); 151-62).
  • Protein GenBank ID GI Number Organism
    prs NP_415725.1  16129170 Escherichia coli
    prsA NP_109761.1  13507812 Mycoplasma pneumoniae M129
    TPK1 BAH19964.1 222424006 Arabidopsis thaliana col
    TPK2 BAH57065.1 227204427 Arabidopsis thaliana col
    • 4.1.1.b Decarboxylase, Alkene Forming
  • Olefin-forming decarboxylase enzymes suitable for converting 3-hydroxypent-4-enoate to butadiene (Step K of FIG. 2) include mevalonate diphosphate decarboxylase (MDD, EC 4.1.1.33) and similar enzymes. MDD participates in the mevalonate pathway for isoprenoid biosynthesis, where it catalyzes the ATP-dependent decarboxylation of mevalonate diphosphate to isopentenyl diphosphate. The MDD enzyme of S. cerevisiae was heterologously expressed in E. coli, where it was shown to catalyze the decarboxylation of 3-hydroxyacids to their corresponding alkenes (WO 2010/001078; Gogerty and Bobik, Appl. Environ. Microbiol., p. 8004-8010, Vol. 76, No. 24 (2010)). Products formed by this enzyme include isobutylene, propylene and ethylene. Two evolved variants of the S. cerevisiae MDD, ScMDD1 (1145F) and ScMDD2 (R74H), achieved 19-fold and 38-fold increases in isobutylene-forming activity compared to the wild-type enzyme (WO 2010/001078). Other exemplary MDD genes are MVD in Homo sapiens and MDD in Staphylococcus aureus and Trypsonoma brucei (Toth et al., J Biol. Chem. 271:7895-7898 (1996); Byres et al., J Mol. Biol. 371:540-553 (2007)).
  • Protein GenBank ID GI Number Organism
    MDD NP_014441.1  6324371 Saccharomyces cerevisiae
    MVD NP_002452.1  4505289 Homo sapiens
    MDD ABQ48418.1 147740120 Staphylococcus aureus
    MDD EAN78728.1  70833224 Trypsonoma brucei
    • 4.1.2.a Aldehyde Lyase
  • The condensation of pyruvate and acetaldehyde to 4-hydroxy-2-oxovalerate (Step B of FIGS. 1 and 2) is catalyzed by 4-hydroxy-2-oxovalerate aldolase (EC 4.1.3.39). This enzyme participates in pathways for the degradation of phenols, cresols and catechols. The E. coli enzyme, encoded by mhpE, is highly specific for acetaldehyde as an acceptor (Pollard et al., Appl Environ Microbiol 64:4093-4094 (1998)). Similar enzymes are encoded by the cmtG and todH genes of Pseudomonas putida (Lau et al., Gene 146:7-13 (1994); Eaton, J Bacteriol. 178:1351-1362 (1996)). In Pseudomonas CF600, this enzyme is part of a bifunctional aldolase-dehydrogenase heterodimer encoded by dmpFG (Manjasetty et al., Acta Crystallogr. D. Biol Crystallogr. 57:582-585 (2001)). The dehydrogenase functionality interconverts acetaldehyde and acetyl-CoA (Step A of FIGS. 1 and 2) and channels the acetaldehyde intermediate to the aldolase. Substrate channeling provides the advantage of reduced cellular concentrations of acetaldehyde, toxic to some cells, and may also reduce acetaldehyde-derived byproducts such as ethanol and acetate. A similar aldolase-dehydrogenase complex is encoded by BphIJ of Burkholderia xenovorans (Baker et al, Biochem 48:6551-8 (2009)).
  • Gene GenBank ID GI Number Organism
    mhpE AAC73455.1 1786548 Escherichia coli
    cmtG AAB62295.1 1263190 Pseudomonas putida
    todH AAA61944.1 485740 Pseudomonas putida
    dmpG CAA43227.1 45684 Pseudomonas sp. CF600
    dmpF CAA43226.1 45683 Pseudomonas sp. CF600
    bphI ABE37049.1 91693852 Burkholderia xenovorans
    bphI ABE37050.1 91693853 Burkholderia xenovorans
    • 4.2.3 Synthase (Alkene Forming)
  • Butadiene Synthase (BDS), shown in Step C of FIG. 4, catalyzes the conversion of 2-butenyl-4-diphosphate to 1,3-butadiene. The enzymes described below naturally possess such activity or can be engineered to exhibit this activity. Carbon-oxygen lyases that operate on phosphates are found in the EC 4.2.3 enzyme class. The table below lists several useful enzymes in EC class 4.2.3.
  • Enzyme Commission Number Enzyme Name
    4.2.3.15 Myrcene synthase
    4.2.3.26 Linalool synthase
    4.2.3.27 Isoprene synthase
    4.2.3.36 Teipentriene sythase
    4.2.3.46 (E,E)-alpha-Famesene synthase
    4.2.3.47 Beta-Famesene synthase
    4.2.3.49 Nerolidol synthase
  • Particularly useful enzymes include isoprene synthase, myrcene synthase and farnesene synthase. Enzyme candidates are described below, and in the enzymes and classes for FIG. 15, Step F.
  • Isoprene synthase naturally catalyzes the conversion of dimethylallyl diphosphate to isoprene, but can also catalyze the synthesis of 1,3-butadiene from 2-butenyl-4-diphosphate. Isoprene synthases can be found in several organisms including Populus alba (Sasaki et al., FEBS Letters, 2005, 579 (11), 2514-2518), Pueraria montana (Lindberg et al., Metabolic Eng, 12(1):70-79 (2010); Sharkey et al., Plant Physiol., 137(2):700-712 (2005)), and Populus tremula x Populus alba, also called Populus canescens (Miller et al., Planta, 2001, 213 (3), 483-487). The crystal structure of the Populus canescens isoprene synthase was determined (Koksal et al, J Mol Biol 402:363-373 (2010)). Additional isoprene synthase enzymes are described in (Chotani et al., WO/2010/031079, Systems Using Cell Culture for Production of Isoprene; Cervin et al., US Patent Application 20100003716, Isoprene Synthase Variants for Improved Microbial Production of Isoprene).
  • Protein GenBank ID GI Number Organism
    ispS BAD98243.1 63108310 Populus alba
    ispS AAQ84170.1 35187004 Pueraria montana
    ispS CAC35696.1 13539551 Populus tremula × Populus alba
  • Myrcene synthase enzymes catalyze the dephosphorylation of geranyl diphosphate to beta-myrcene (EC 4.2.3.15). Exemplary myrcene synthases are encoded by MST2 of Solanum lycopersicum (van Schie et al, Plant Mol Biol 64:D473-79 (2007)), TPS-Myr of Picea abies (Martin et al, Plant Physiol 135:1908-27 (2004)) g-myr of Abies grandis (Bohlmann et al, J Biol Chem 272:21784-92 (1997)) and TPS10 of Arabidopsis thaliana (Bohlmann et al, Arch Biochem Biophys 375:261-9 (2000)). These enzymes were heterologously expressed in E. coli.
  • Protein GenBank ID GI Number Organism
    MST2 ACN58229.1 224579303 Solanum lycopersicum
    TPS-Myr AAS47690.2  77546864 Picea abies
    G-myr O24474.1  17367921 Abies grandis
    TPS10 EC07543.1 330252449 Arabidopsis thaliana
  • Farnesyl diphosphate is converted to alpha-farnesene and beta-farnesene by alpha-farnesene synthase and beta-farnesene synthase, respectively. Exemplary alpha-farnesene synthase enzymes include TPS03 and TPS02 of Arabidopsis thaliana (Faldt et al, Planta 216:745-51 (2003); Huang et al, Plant Physiol 153:1293-310 (2010)), afs of Cucumis sativus (Mercke et al, Plant Physiol 135:2012-14 (2004), eafar of Malus x domestica (Green et al, Phytochem 68:176-88 (2007)) and TPS-Far of Picea abies (Martin, supra). An exemplary beta-farnesene synthase enzyme is encoded by TPS1 of Zea mays (Schnee et al, Plant Physiol 130:2049-60 (2002)).
  • Protein GenBank ID GI Number Organism
    TPS03 A4FVP2.1 205829248 Arabidopsis thaliana
    TPS02 P0CJ43.1 317411866 Arabidopsis thaliana
    TPS-Far AAS47697.1  44804601 Picea abies
    afs AAU05951.1  51537953 Cucumis sativus
    eafar Q84LB2.2  75241161 Malus × domestica
    TPS1 Q84ZW8.1  75149279 Zea mays
    • 6.2.1 CoA Synthetases and Ligases
  • The activation of pathway intermediates such as 2-oxopent-3-enoate (1N), 2-hydroxypent-3-enoate (1R), 5-hydroxypent-4-enoate (2I), 2,4-pentadienoate (2J), 3-hydroxypent-4-enoate (2L) can be catalyzed by ADP and AMP-forming CoA ligases (6.2.1). These enzymes can also function in the reverse direction to convert the CoA-derivatives to their acid counterparts as shown in Steps 1Q, 3B, 3I, 3J and 3N.
  • Several enzymes with broad substrate specificities have been described in the literature. The ADP-forming acetyl-CoA synthetase (ACD, EC 6.2.1.13) from Archaeoglobus fulgidus, encoded by AF1211, was shown to operate on a variety of linear and branched-chain substrates including isobutyrate, isopentanoate, and fumarate (Musfeldt et al., J Bacteriol. 184:636-644 (2002)). A second reversible ACD in Archaeoglobus fulgidus, encoded by AF1983, was also indicated to have a broad substrate range (Musfeldt et al., supra). The enzyme from Haloarcula marismortui, annotated as a succinyl-CoA synthetase, accepts propionate, butyrate, and branched-chain acids (isovalerate and isobutyrate) as substrates, and was shown to operate in the forward and reverse directions (Brasen et al., Arch. Microbiol 182:277-287 (2004)). The ACD encoded by PAE3250 from hyperthermophilic crenarchaeon Pyrobaculum aerophilum showed the broadest substrate range of all characterized ACDs, reacting with acetyl-CoA, isobutyryl-CoA (preferred substrate) and phenylacetyl-CoA (Brasen and Schonheit, Arch. Microbiol 182:277-287 (2004)). Directed evolution or engineering can be used to modify this enzyme to operate at the physiological temperature of the host organism. The enzymes from A. fulgidus, H. marismortui and P. aerophilum have all been cloned, functionally expressed, and characterized in E. coli (Brasen and Schonheit, Arch. Microbiol 182:277-287 (2004); Musfeldt and Schonheit, J Bacteriol. 184:636-644 (2002)). An additional enzyme is encoded by sucCD in E. coli, which naturally catalyzes the formation of succinyl-CoA from succinate with the concomitant consumption of one ATP, a reaction which is reversible in vivo (Buck et al., Biochemistry 24:6245-6252 (1985)). The acyl CoA ligase from Pseudomonas putida has been indicated to work on several aliphatic substrates including acetic, propionic, butyric, valeric, hexanoic, heptanoic, and octanoic acids and on aromatic compounds such as phenylacetic and phenoxyacetic acids (Fernandez-Valverde et al., Appl. Environ. Microbiol. 59:1149-1154 (1993)). A related enzyme, malonyl CoA synthetase (6.3.4.9) from Rhizobium leguminosarum could convert several diacids, namely, ethyl-, propyl-, allyl-, isopropyl-, dimethyl-, cyclopropyl-, cyclopropylmethylene-, cyclobutyl-, and benzyl-malonate into their corresponding monothioesters (Pohl et al., J. Am. Chem. Soc. 123:5822-5823 (2001)). Recently, a CoA dependent acetyl-CoA ligase was also identified in Propionibacterium acidipropionici ATCC 4875 (Parizzi et al., BMC Genomics. 2012; 13: 562. The genome sequence of Propionibacterium acidipropionici provides insights into its biotechnological and industrial potential). This enzyme is distinct from the AMP-dependent acetyl-CoA synthetase and is instead related to the ADP-forming succinyl-CoA synthetase complex (SCSC). Genes related to the SCSC (α and β subunits) complex were also found in Propionibacterium acnes KPA171202 and Microlunatus phophovorus NM-1.
  • The acylation of acetate to acetyl-CoA is catalyzed by enzymes with acetyl-CoA synthetase activity. Two enzymes that catalyze this reaction are AMP-forming acetyl-CoA synthetase (EC 6.2.1.1) and ADP-forming acetyl-CoA synthetase (EC 6.2.1.13). AMP-forming acetyl-CoA synthetase (ACS) is the predominant enzyme for activation of acetate to acetyl-CoA. Exemplary ACS enzymes are found in E. coli (Brown et al., J. Gen. Microbiol 102:327-336 (1977)), Ralstonia eutropha (Priefert et al., 174:6590-6599 (1992)), Methanothermobacter thermautotrophicus (Ingram-Smith et al., Archaea. 2:95-107 (2007)), Salmonella enterica (Gulick et al., 42:2866-2873 (2003)) and Saccharomyces cerevisiae (Jogl et al., 43:1425-1431 (2004)).
  • Methylmalonyl-CoA synthetase from Rhodopseudomonas palustris (MatB) converts methylmalonate and malonate to methylmalonyl-CoA and malonyl-CoA, respectively. Structure-based mutagenesis of this enzyme improved CoA synthetase activity with the alternate substrates ethylmalonate and butylmalonate (Crosby et al, AEM, in press (2012)).
  • GenBank Accession
    Gene No. GI No. Organism
    AF1211 NP_070039.1  11498810 Archaeoglobus fulgidus
    AF1983 NP_070807.1  11499565 Archaeoglobus fulgidus
    Scs YP_135572.1  55377722 Haloarcula marismortui
    PAE3250 NP_560604.1  18313937 Pyrobaculum aerophilum
    str. IM2
    sucC NP_415256.1  16128703 Escherichia coli
    sucD AAC73823.1  1786949 Escherichia coli
    paaF AAC24333.2  22711873 Pseudomonas putida
    matB AAC83455.1  3982573 Rhizobium leguminosarum
    Acs AAC77039.1  1790505 Escherichia coli
    acoE AAA21945.1   141890 Ralstonia eutropha
    acs1 ABC87079.1  86169671 Methanothermobacter
    thermautotrophicus
    acs1 AAL23099.1  16422835 Salmonella enterica
    ACS1 Q01574.2 257050994 Saccharomyces cerevisiae
    LSC1 NP_014785  6324716 Saccharomyces cerevisiae
    LSC2 NP_011760  6321683 Saccharomyces cerevisiae
    bioW NP_390902.2  50812281 Bacillus subtilis
    bioW CAA10043.1  3850837 Pseudomonas mendocina
    bioW P22822.1   115012 Bacillus sphaericus
    Phl CAJ15517.1  77019264 Penicillium chrysogenum
    phlB ABS19624.1 152002983 Penicillium chrysogenum
    paaF AAC24333.2  22711873 Pseudomonas putida
    PACID_ YP_006979420.1 410864809 Propionibacterium
    02150 acidipropionici
    ATCC 4875
    PPA1754 AAT83483.1  50840816 Propionibacterium acnes
    KPA171202
    PPA1755 AAT83484.1  50840817 Propionibacterium acnes
    KPA171202
    Subunit YP_004571669.1 336116902 Microlunatus phosphovorus
    alpha NM-1
    Subunit YP_004571668.1 336116901 Microlunatus phosphovorus
    beta NM-1
    AACS NP_084486.1  21313520 Mus musculus
    AACS NP_076417.2  31982927 Homo sapiens
  • 4HB-CoA synthetase catalyzes the ATP-dependent conversion of 4-hydroxybutyrate to 4-hydroxybutyryl-CoA. AMP-forming 4-HB-CoA synthetase enzymes are found in organisms that assimilate carbon via the dicarboxylate/hydroxybutyrate cycle or the 3-hydroxypropionate/4-hydroxybutyrate cycle. Enzymes with this activity have been characterized in Thermoproteus neutrophilus and Metallosphaera sedula (Ramos-Vera et al, J Bacteriol 192:5329-40 (2010); Berg et al, Science 318:1782-6 (2007)). Others can be inferred by sequence homology.
  • Protein GenBank ID GI Number Organism
    Tneu_0420 ACB39368.1 170934107 Thermoproteus
    neutrophilus
    Caur_0002 YP_001633649.1 163845605 Chloroflexus aurantiacus
    J-10-fl
    Cagg_3790 YP_002465062 219850629 Chloroflexus aggregans
    DSM 9485
    Acs YP_003431745 288817398 Hydrogenobacter
    thermophilus TK-6
    Pisl_0250 YP_929773.1 119871766 Pyrobaculum islandicum
    DSM 4184
    Msed_1422 ABP95580.1 145702438 Metallosphaera sedula
    • 3.1.2: CoA Hydrolases
  • CoA hydrolysis as described in Steps 1Q, 2J and 2I can be catalyzed by CoA hydrolases or thioesterases in the EC class 3.1.2. Additionally, intermediates such as 3-oxo-4-hydroxypentanoyl-CoA, 3,4-dihydroxypentanoyl-CoA, 4-hydroxypent-2-enoyl-COA, 2,4-pentadienoyl-CoA can be converted into their acid counterparts via thes enzymes as shown in steps 3B, 3I, 3J, 3N respectively. Several CoA hydrolases with broad substrate ranges are suitable enzymes for hydrolyzing these intermediates. For example, the enzyme encoded by acot12 from Rattus norvegicus brain (Robinson et al., Biochem. Biophys. Res. Commun. 71:959-965 (1976)) can react with butyryl-CoA, hexanoyl-CoA and malonyl-CoA. The human dicarboxylic acid thioesterase, encoded by acot8, exhibits activity on glutaryl-CoA, adipyl-CoA, suberyl-CoA, sebacyl-CoA, and dodecanedioyl-CoA (Westin et al., J. Biol. Chem. 280:38125-38132 (2005)). The closest E. coli homolog to this enzyme, tesB, can also hydrolyze a range of CoA thiolesters (Naggert et al., J Biol Chem 266:11044-11050 (1991)). A similar enzyme has also been characterized in the rat liver (Deana R., Biochem Int 26:767-773 (1992)). Additional enzymes with hydrolase activity in E. coli include ybgC, paaI, yciA, and ybdB (Kuznetsova, et al., FEMS Microbiol Rev, 2005, 29(2):263-279; Song et al., J Biol Chem, 2006, 281(16):11028-38). Though its sequence has not been reported, the enzyme from the mitochondrion of the pea leaf has a broad substrate specificity, with demonstrated activity on acetyl-CoA, propionyl-CoA, butyryl-CoA, palmitoyl-CoA, oleoyl-CoA, succinyl-CoA, and crotonyl-CoA (Zeiher et al., Plant. Physiol. 94:20-27 (1990)) The acetyl-CoA hydrolase, ACH1, from S. cerevisiae represents another candidate hydrolase (Buu et al., J. Biol. Chem. 278:17203-17209 (2003)).
  • Gene name GenBank Accession # GI # Organism
    acot12 NP_570103.1 18543355 Rattus norvegicus
    tesB NP_414986 16128437 Escherichia coli
    acot8 CAA15502  3191970 Homo sapiens
    acot8 NP_570112 51036669 Rattus norvegicus
    tesA NP_415027 16128478 Escherichia coli
    ybgC NP_415264 16128711 Escherichia coli
    paaI NP_415914 16129357 Escherichia coli
    ybdB NP_415129 16128580 Escherichia coli
    ACH1 NP_009538  6319456 Saccharomyces cerevisiae
    yciA NP_415769.1 16129214 Escherichia coli
    ydiI P77781.1 13878877 Escherichia coli
    ybfF P75736.1  2829622 Escherichia coli
  • Yet another candidate hydrolase is the glutaconate CoA-transferase from Acidaminococcus fermentans. This enzyme was transformed by site-directed mutagenesis into an acyl-CoA hydrolase with activity on glutaryl-CoA, acetyl-CoA and 3-butenoyl-CoA (Mack et al., FEBS. Lett. 405:209-212 (1997)). This suggests that the enzymes encoding succinyl-CoA:3-ketoacid-CoA transferases and acetoacetyl-CoA:acetyl-CoA transferases may also serve as candidates for this reaction step but would require certain mutations to change their function.
  • Gene name GenBank Accession # GI # Organism
    gctA CAA57199 559392 Acidaminococcus fermentans
    gctB CAA57200 559393 Acidaminococcus fermentans
  • Additional hydrolase enzymes include 3-hydroxyisobutyryl-CoA hydrolase which has been described to efficiently catalyze the conversion of 3-hydroxyisobutyryl-CoA to 3-hydroxyisobutyrate during valine degradation (Shimomura et al., J Biol Chem. 269:14248-14253 (1994)). Genes encoding this enzyme include hibch of Rattus norvegicus (Shimomura et al., Methods Enzymol. 324:229-240 (2000)) and Homo sapiens (Shimomura et al., supra). Similar gene candidates can also be identified by sequence homology, including hibch of Saccharomyces cerevisiae and BC_2292 of Bacillus cereus.
  • Gene name GenBank Accession # GI # Organism
    hibch Q5XIE6.2 146324906 Rattus norvegicus
    hibch Q6NVY1.2 146324905 Homo sapiens
    hibch P28817.2  2506374 Saccharomyces cerevisiae
    BC_2292 AP09256  29895975 Bacillus cereus
  • Methylmalonyl-CoA is converted to methylmalonate by methylmalonyl-CoA hydrolase (EC 3.1.2.7). This enzyme, isolated from Rattus norvegicus liver, is also active on malonyl-CoA and propionyl-CoA as alternative substrates (Kovachy et al., J. Biol. Chem., 258: 11415-11421 (1983)). The gene associated with this enzyme is not known.
    • 2.8.3 CoA Transferase
  • Several transformations outlined in FIGS. 1, 2 and 3 require a CoA transferase to activate carboxylic acids to their corresponding acyl-CoA derivatives and vice versa. The specific transformations are shown in steps 1N, 1R, 1Q, 2I, 2J, 2L, 3B, 3I, 3J.
  • CoA transferase enzymes have been described in the open literature and represent suitable candidates for these steps. These are described below. The gene products of cat1, cat2, and cat3 of Clostridium kluyveri have been shown to exhibit succinyl-CoA, 4-hydroxybutyryl-CoA, and butyryl-CoA transferase activity, respectively (Seedorf et al., Proc. Natl. Acad. Sci U.S.A 105:2128-2133 (2008); Sohling et al., J Bacteriol. 178:871-880 (1996)). Similar CoA transferase activities are also present in Trichomonas vaginalis, Trypanosoma brucei, Clostridium aminobutyricum and Porphyromonas gingivalis (Riviere et al., J. Biol. Chem. 279:45337-45346 (2004); van Grinsven et al., J. Biol. Chem. 283:1411-1418 (2008)).
  • Protein GenBank ID GI Number Organism
    cat1 P38946.1   729048 Clostridium kluyveri
    cat2 P38942.2 172046066 Clostridium kluyveri
    cat3 EDK35586.1 146349050 Clostridium kluyveri
    TVAG_395550 XP_001330176 123975034 Trichomonas vaginalis G3
    Tb11.02.0290 XP_828352  71754875 Trypanosoma brucei
    cat2 CAB60036.1  6249316 Clostridium
    aminobutyricum
    cat2 NP_906037.1  34541558 Porphyromonas gingivalis
    W83
  • A fatty acyl-CoA transferase that utilizes acetyl-CoA as the CoA donor is acetoacetyl-CoA transferase, encoded by the E. coli atoA (alpha subunit) and atoD (beta subunit) genes (Korolev et al., Acta Crystallogr. D. Biol. Crystallogr. 58:2116-2121 (2002); Vanderwinkel et al., 33:902-908 (1968)). This enzyme has a broad substrate range on substrates of chain length C3-C6 (Sramek et al., Arch Biochem Biophys 171:14-26 (1975)) and has been shown to transfer the CoA moiety to acetate from a variety of branched and linear 3-oxo and acyl-CoA substrates, including isobutyrate (Matthies et al., Appl Environ. Microbiol 58:1435-1439 (1992)), valerate (Vanderwinkel et al., Biochem. Biophys. Res. Commun. 33:902-908 (1968)) and butanoate (Vanderwinkel et al., Biochem. Biophys. Res. Commun. 33:902-908 (1968)). This enzyme is induced at the transcriptional level by acetoacetate, so modification of regulatory control may be necessary for engineering this enzyme into a pathway (Pauli et al., Eur. J Biochem. 29:553-562 (1972)). Similar enzymes exist in Corynebacterium glutamicum ATCC 13032 (Duncan et al., 68:5186-5190 (2002)), Clostridium acetobutylicum (Cary et al., Appl Environ Microbiol 56:1576-1583 (1990); Wiesenborn et al., Appl Environ Microbiol 55:323-329 (1989)), and Clostridium saccharoperbutylacetonicum (Kosaka et al., Biosci. Biotechnol Biochem. 71:58-68 (2007)).
  • Gene GI # Accession No. Organism
    atoA  2492994 P76459.1 Escherichia coli
    atoD  2492990 P76458.1 Escherichia coli
    actA 62391407 YP_226809.1 Corynebacterium glutamicum
    cg0592 62389399 YP_224801.1 Corynebacterium glutamicum
    ctfA 15004866 NP_149326.1 Clostridium acetobutylicum
    ctfB 15004867 NP_149327.1 Clostridium acetobutylicum
    ctfA 31075384 AAP42564.1 Clostridium
    saccharoperbutylacetonicum
    ctfB 31075385 AAP42565.1 Clostridium
    saccharoperbutylacetonicum
    • 4.1.1 Decarboxylase
  • Exemplary enzymes for catalyzing the decarboxylation of 2,4-pentadienoate (1H, 2G, 3L), 4-hydroxypent-2-enoate (1T), 5-hydroxypent-2-enoate (2P) and 4-hydroxypent-2-enoate (3E) are sorbic acid decarboxylase, aconitate decarboxylase, 4-oxalocrotonate decarboxylase and cinnamate decarboxylase.
  • Sorbic acid decarboxylase converts sorbic acid to 1,3-pentadiene. Sorbic acid decarboxylation by Aspergillus niger requires three genes: padA1, ohbA1, and sdrA (Plumridge et al. Fung. Genet. Bio, 47:683-692 (2010). PadA1 is annotated as a phenylacrylic acid decarboxylase, ohbA1 is a putative 4-hydroxybenzoic acid decarboxylase, and sdrA is a sorbic acid decarboxylase regulator. Additional species have also been shown to decarboxylate sorbic acid including several fungal and yeast species (Kinderlerler and Hatton, Food Addit Contam., 7(5):657-69 (1990); Casas et al., Int J Food Micro., 94(1):93-96 (2004); Pinches and Apps, Int. J. Food Microbiol. 116: 182-185 (2007)). For example, Aspergillus oryzae and Neosartorya fischeri have been shown to decarboxylate sorbic acid and have close homologs to padA1, ohbA1, and sdrA.
  • Gene name GenBankID GI Number Organism
    padA1 XP_001390532.1 145235767 Aspergillus niger
    ohbA1 XP_001390534.1 145235771 Aspergillus niger
    sdrA XP_001390533.1 145235769 Aspergillus niger
    padA1 XP_001818651.1 169768362 Aspergillus oryzae
    ohbA1 XP_001818650.1 169768360 Aspergillus oryzae
    sdrA XP_001818649.1 169768358 Aspergillus oryzae
    padA1 XP_001261423.1 119482790 Neosartorya fischeri
    ohbA1 XP_001261424.1 119482792 Neosartorya fischeri
    sdrA XP_001261422.1 119482788 Neosartorya fischeri
  • Aconitate decarboxylase (EC 4.1.1.6) catalyzes the final step in itaconate biosynthesis in a strain of Candida and also in the filamentous fungus Aspergillus terreus (Bonnarme et al. J Bacteriol. 177:3573-3578 (1995); Willke and Vorlop, Appl Microbiol. Biotechnol 56:289-295 (2001)). A cis-aconitate decarboxylase (CAD) (EC 4.1.16) has been purified and characterized from Aspergillus terreus (Dwiarti et al., J. Biosci. Bioeng. 94(1): 29-33 (2002)). Recently, the gene has been cloned and functionally characterized (Kanamasa et al., Appl. Microbiol Biotechnol 80:223-229 (2008)) and (WO/2009/014437). Several close homologs of CAD are listed below (EP 2017344A1; WO 2009/014437 A1). The gene and protein sequence of CAD were reported previously (EP 2017344 A1; WO 2009/014437 A1), along with several close homologs listed in the table below.
  • Gene name GenBankID GI Number Organism
    CAD XP_001209273 115385453 Aspergillus terreus
    XP_001217495 115402837 Aspergillus terreus
    XP_001209946 115386810 Aspergillus terreus
    BAE66063  83775944 Aspergillus oryzae
    XP_001393934 145242722 Aspergillus niger
    XP_391316  46139251 Gibberella zeae
    XP_001389415 145230213 Aspergillus niger
    XP_001383451 126133853 Pichia stipitis
    YP_891060 118473159 Mycobacterium smegmatis
    NP_961187  41408351 Mycobacterium avium
    subsp. pratuberculosis
    YP_880968 118466464 Mycobacterium avium
    ZP_01648681 119882410 Salinispora arenicola
  • An additional class of decarboxylases has been characterized that catalyze the conversion of cinnamate (phenylacrylate) and substituted cinnamate derivatives to the corresponding styrene derivatives. These enzymes are common in a variety of organisms and specific genes encoding these enzymes that have been cloned and expressed in E. coli are: pad 1 from Saccharomyces cerevisae (Clausen et al., Gene 142:107-112 (1994)), pdc from Lactobacillus plantarum (Barthelmebs et al., 67:1063-1069 (2001); Qi et al., Metab Eng 9:268-276 (2007); Rodriguez et al., J. Agric. Food Chem. 56:3068-3072 (2008)), pofK (pad) from Klebsiella oxytoca (Uchiyama et al., Biosci. Biotechnol. Biochem. 72:116-123 (2008); Hashidoko et al., Biosci. Biotech. Biochem. 58:217-218 (1994)), Pedicoccus pentosaceus (Barthelmebs et al., 67:1063-1069 (2001)), and padC from Bacillus subtilis and Bacillus pumilus (Shingler et al., 174:711-724 (1992)). A ferulic acid decarboxylase from Pseudomonas fluorescens also has been purified and characterized (Huang et al., J. Bacteriol. 176:5912-5918 (1994)). Importantly, this class of enzymes have been shown to be stable and do not require either exogenous or internally bound co-factors, thus making these enzymes ideally suitable for biotransformations (Sariaslani, Annu. Rev. Microbiol. 61:51-69 (2007)).
  • Protein GenBank ID GI Number Organism
    pad1 AAB64980.1  1165293 Saccharomyces cerevisae
    ohbA1 BAG32379.1 188496963 Saccharomyces cerevisiae
    pdc AAC45282.1  1762616 Lactobacillus plantarum
    pad BAF65031.1 149941608 Klebsiella oxytoca
    padC NP_391320.1  16080493 Bacillus subtilis
    pad YP_804027.1 116492292 Pedicoccus pentosaceus
    pad CAC18719.1  11691810 Bacillus pumilus
  • 4-Oxalocronate decarboxylase catalyzes the decarboxylation of 4-oxalocrotonate to 2-oxopentanoate. This enzyme has been isolated from numerous organisms and characterized. The decarboxylase typically functions in a complex with vinylpyruvate hydratase. Genes encoding this enzyme include dmpH and dmpE in Pseudomonas sp. (strain 600) (Shingler et al., 174:711-724 (1992)), xylII and xylIII from Pseudomonas putida (Kato et al., Arch. Microbiol 168:457-463 (1997); Stanley et al., Biochemistry 39:3514 (2000); Lian et al., J. Am. Chem. Soc. 116:10403-10411 (1994)) and Reut_B5691 and Reut_B5692 from Ralstonia eutropha JMP134 (Hughes et al., J Bacteriol, 158:79-83 (1984)). The genes encoding the enzyme from Pseudomonas sp. (strain 600) have been cloned and expressed in E. coli (Shingler et al., J. Bacteriol. 174:711-724 (1992)). The 4-oxalocrotonate decarboxylase encoded by xylI in Pseudomonas putida functions in a complex with vinylpyruvate hydratase. A recombinant form of this enzyme devoid of the hydratase activity and retaining wild type decarboxylase activity has been characterized (Stanley et al., Biochem. 39:718-26 (2000)). A similar enzyme is found in Ralstonia pickettii (formerly Pseudomonas pickettii) (Kukor et al., J Bacteriol. 173:4587-94 (1991)).
  • Gene GenBank GI Number Organism
    dmpH CAA43228.1   45685 Pseudomonas sp. CF600
    dmpE CAA43225.1   45682 Pseudomonas sp. CF600
    xylII YP_709328.1 111116444 Pseudomonas putida
    xylIII YP_709353.1 111116469 Pseudomonas putida
    Reut_B5691 YP_299880.1  73539513 Ralstonia eutropha
    JMP134
    Reut_B5692 YP_299881.1  73539514 Ralstonia eutropha
    JMP134
    xylI P49155.1  1351446 Pseudomonas putida
    tbuI YP_002983475.1 241665116 Ralstonia pickettii
    nbaG BAC65309.1  28971626 Pseudomonas fluorescens
    KU-7
  • Numerous characterized enzymes decarboxylate amino acids and similar compounds, including aspartate decarboxylase, lysine decarboxylase and ornithine decarboxylase. Aspartate decarboxylase (EC 4.1.1.11) decarboxylates aspartate to form beta-alanine. This enzyme participates in pantothenate biosynthesis and is encoded by gene panD in Escherichia coli (Dusch et al., Appl. Environ. Microbiol 65:1530-1539 (1999); Ramjee et al., Biochem. J 323 (Pt 3):661-669 (1997); Merkel et al., FEMS Microbiol Lett. 143:247-252 (1996); Schmitzberger et al., EMBO J 22:6193-6204 (2003)). The enzymes from Mycobacterium tuberculosis (Chopra et al., Protein Expr. Purif. 25:533-540 (2002)) and Corynebacterium glutanicum (Dusch et al., Appl. Environ. Microbiol 65:1530-1539 (1999)) have been expressed and characterized in E. coli.
  • Protein GenBank ID GI Number Organism
    panD P0A790 67470411 Escherichia coli K12
    panD Q9X4N0 18203593 Corynebacterium glutanicum
    panD P65660.1 54041701 Mycobacterium tuberculosis
  • Lysine decarboxylase (EC 4.1.1.18) catalyzes the decarboxylation of lysine to cadaverine. Two isozymes of this enzyme are encoded in the E. coli genome by genes cadA and ldcC. CadA is involved in acid resistance and is subject to positive regulation by the cadC gene product (Lemonnier et al., Microbiology 144 (Pt 3):751-760 (1998)). CadC accepts hydroxylysine and S-aminoethylcysteine as alternate substrates, and 2-aminopimelate and 6-aminocaproate act as competitive inhibitors to this enzyme (Sabo et al., Biochemistry 13:662-670 (1974)). The constitutively expressed lde gene product is less active than CadA (Lemonnier and Lane, Microbiology 144 (Pt 3):751-760 (1998)). A lysine decarboxylase analogous to CadA was recently identified in Vibrio parahaemolyticus (Tanaka et al., J Appl Microbiol 104:1283-1293 (2008)). The lysine decarboxylase from Selenomonas ruminantium, encoded by ldc, bears sequence similarity to eukaryotic ornithine decarboxylases, and accepts both L-lysine and L-ornithine as substrates (Takatsuka et al., Biosci. Biotechnol Biochem. 63:1843-1846 (1999)). Active site residues were identified and engineered to alter the substrate specificity of the enzyme (Takatsuka et al., J Bacteriol. 182:6732-6741 (2000)). Several ornithine decarboxylase enzymes (EC 4.1.1.17) also exhibit activity on lysine and other similar compounds. Such enzymes are found in Nicotiana glutinosa (Lee et al., Biochem. J 360:657-665 (2001)), Lactobacillus sp. 30a (Guirard et al., J Biol. Chem. 255:5960-5964 (1980)) and Vibrio vulnificus (Lee et al., J Biol. Chem. 282:27115-27125 (2007)). The enzymes from Lactobacillus sp. 30a (Momany et al., J Mol. Biol. 252:643-655 (1995)) and V. vulnificus have been crystallized. The V. vulnificus enzyme efficiently catalyzes lysine decarboxylation and the residues involved in substrate specificity have been elucidated (Lee et al., J Biol. Chem. 282:27115-27125 (2007)). A similar enzyme has been characterized in Trichomonas vaginalis but the gene encoding this enzyme is not known (Yarlett et al., Biochem. J 293 (Pt 2):487-493 (1993)).
  • Protein GenBank ID GI Number Organism
    cadA AAA23536.1  145458 Escherichia coli
    ldcC AAC73297.1  1786384 Escherichia coli
    Ldc O50657.1 13124043 Selenomonas ruminantium
    cadA AB124819.1 44886078 Vibrio parahaemolyticus
    AF323910.1: AAG45222.1 12007488 Nicotiana glutinosa
    1 . . . 1299
    odc1 P43099.2  1169251 Lactobacillus sp. 30a
    VV2_1235 NP_763142.1 27367615 Vibrio vulnificus
  • An exemplary carboxy-lyase for decarboxylating 2-hydroxypent-3-enoate (1S) is acetolactate decarboxylase (4.1.1.5) which participates in citrate catabolism and branched-chain amino acid biosynthesis, converting the 2-hydroxyacid, 2-acetolactate, to acetoin. In Lactococcus lactis the enzyme is composed of six subunits, encoded by gene aldB, and is activated by valine, leucine and isoleucine (Goupil-Feuillerat et al., J. Bacteriol. 182:5399-5408 (2000); Goupil et al., Appl. Environ. Microbiol. 62:2636-2640 (1996)). This enzyme has been overexpressed and characterized in E. coli (Phalip et al., FEBS Lett. 351:95-99 (1994); Nielsen et al, Biotechnol J 5:274-84 (2010)). In other organisms the enzyme is a dimer, encoded by aldC in Streptococcus thermophilus (Monnet et al., Lett. Appl. Microbiol. 36:399-405 (2003)), aldB in Bacillus brevis (Najmudin et al., Acta Crystallogr. D. Biol. Crystallogr. 59:1073-1075 (2003); Diderichsen et al., J. Bacteriol. 172:4315-4321 (1990)) and budA from Enterobacter aerogenes (Diderichsen et al., J. Bacteriol. 172:4315-4321 (1990)). The enzyme from Bacillus brevis was cloned and overexpressed in Bacillus subtilis and characterized crystallographically (Najmudin et al., Acta Crystallogr. D. Biol. Crystallogr. 59:1073-1075 (2003)). The Acetobacter aceti acetolactate decarboxylase was cloned and heterologously expressed in brewer's yeast (Yamano et al, J Biotechnol 32:165-71 (1994)). Additionally, the enzyme from Leuconostoc lactis has been purified and characterized but the gene has not been isolated (O'Sullivan et al., FEMS Microbiol. Lett. 194:245-249 (2001)).
  • Gene name GenBank Accession # GI # Organism
    aldB AAB81923.1 2565161 Lactococcus lactis
    aldC Q8L208 Streptococcus thermophilus
    aldB P23616 Bacillus brevis
    budA P05361 Enterobacter aerogenes
    aldc AAC60472.1  545933 Acetobacter aceti
  • Tartrate decarboxylase (EC 4.1.1.73) carries out an alpha,beta-hydroxyacid decarboxylation reaction. The enzyme, characterized in Pseudomonas sp. group Ve-2, is NAD+ dependent and catalyzes coupled oxidation-reduction reaction that proceeds through an oxaloglycolate intermediate (Furuyoshi et al., J Biochem. 110:520-525 (1991)). A side reaction catalyzed by this enzyme is the NAD+ dependent oxidation of tartrate (1% of activity). A gene has not been associated with this enzyme activity to date.
  • The decarboxylation of keto-acids such as 4-hydroxy 2-oxovalerate, 2-oxopent-3-enoate as shown in Steps 1C and 1I is catalyzed by a variety of enzymes with varied substrate specificities, including pyruvate decarboxylase (EC 4.1.1.1), benzoylformate decarboxylase (EC 4.1.1.7), alpha-ketoglutarate decarboxylase and branched-chain alpha-ketoacid decarboxylase. Pyruvate decarboxylase (PDC), also termed keto-acid decarboxylase, is a key enzyme in alcoholic fermentation, catalyzing the decarboxylation of pyruvate to acetaldehyde. The enzyme from Saccharomyces cerevisiae has a broad substrate range for aliphatic 2-keto acids including 2-ketobutyrate, 2-ketovalerate, 3-hydroxypyruvate and 2-phenylpyruvate (22). This enzyme has been extensively studied, engineered for altered activity, and functionally expressed in E. coli (Killenberg-Jabs et al., Eur. J. Biochem. 268:1698-1704 (2001); Li et al., Biochemistry. 38:10004-10012 (1999); ter Schure et al., Appl. Environ. Microbiol. 64:1303-1307 (1998)). The PDC from Zymomonas mobilus, encoded by pdc, also has a broad substrate range and has been a subject of directed engineering studies to alter the affinity for different substrates (Siegert et al., Protein Eng Des Sel 18:345-357 (2005)). The crystal structure of this enzyme is available (Killenberg-Jabs et al., Eur. J. Biochem. 268:1698-1704 (2001)). Other well-characterized PDC candidates include the enzymes from Acetobacter pasteurians (Chandra et al., 176:443-451 (2001)) and Kluyveromyces lactis (Krieger et al., 269:3256-3263 (2002)).
  • Protein GenBank ID GI Number Organism
    pdc P06672.1  118391 Zymomonas mobilis
    pdc1 P06169 30923172 Saccharomyces cerevisiae
    pdc Q8L388 20385191 Acetobacter pasteurians
    pdc1 Q12629 52788279 Kluyveromyces lactis
  • Like PDC, benzoylformate decarboxylase (EC 4.1.1.7) has a broad substrate range and has been the target of enzyme engineering studies. The enzyme from Pseudomonas putida has been extensively studied and crystal structures of this enzyme are available (Polovnikova et al., 42:1820-1830 (2003); Hasson et al., 37:9918-9930 (1998)). Site-directed mutagenesis of two residues in the active site of the Pseudomonas putida enzyme altered the affinity (Km) of naturally and non-naturally occurring substrates (Siegert et al., Protein Eng Des Sel 18:345-357 (2005)). The properties of this enzyme have been further modified by directed engineering (Lingen et al., Chembiochem. 4:721-726 (2003); Lingen et al., Protein Eng 15:585-593 (2002)). The enzyme from Pseudomonas aeruginosa, encoded by mdlC, has also been characterized experimentally (Barrowman et al., 34:57-60 (1986)). Additional gene candidates from Pseudomonas stutzeri, Pseudomonas fluorescens and other organisms can be inferred by sequence homology or identified using a growth selection system developed in Pseudomonas putida (Henning et al., Appl. Environ. Microbiol. 72:7510-7517 (2006)).
  • Protein GenBank ID GI Number Organism
    mdlC P20906.2 3915757 Pseudomonas putida
    mdlC Q9HUR2.1 81539678 Pseudomonas aeruginosa
    dpgB ABN80423.1 126202187 Pseudomonas stutzeri
    ilvB-1 YP_260581.1 70730840 Pseudomonas fluorescens
  • A third enzyme capable of decarboxylating 2-oxoacids is alpha-ketoglutarate decarboxylase (KGD, EC 4.1.1.71). The substrate range of this class of enzymes has not been studied to date. An exemplarly KDC is encoded by kgd in Mycobacterium tuberculosis (Tian et al., PNAS 102:10670-10675 (2005)). KDC enzyme activity has also been detected in several species of rhizobia including Bradyrhizobium japonicum and Mesorhizobium loti (Green et al., J Bacteriol 182:2838-2844 (2000)). Although the KDC-encoding gene(s) have not been isolated in these organisms, the genome sequences are available and several genes in each genome are annotated as putative KDCs. A KDC from Euglena gracilis has also been characterized but the gene associated with this activity has not been identified to date (Shigeoka et al., Arch. Biochem. Biophys. 288:22-28 (1991)). The first twenty amino acids starting from the N-terminus were sequenced MTYKAPVKDVKFLLDKVFKV (Shigeoka and Nakano, Arch. Biochem. Biophys. 288:22-28 (1991)). The gene could be identified by testing candidate genes containing this N-terminal sequence for KDC activity.
  • Protein GenBank ID GI Number Organism
    kgd O50463.4 160395583 Mycobacterium tuberculosis
    kgd NP_767092.1 27375563 Bradyrhizobium japonicum USDA110
    kgd NP_105204.1 13473636 Mesorhizobium loti
  • A fourth candidate enzyme for catalyzing this reaction is branched chain alpha-ketoacid decarboxylase (BCKA). This class of enzyme has been shown to act on a variety of compounds varying in chain length from 3 to 6 carbons (Oku et al., J Biol Chem. 263:18386-18396 (1988); Smit et al., Appl Environ Microbiol 71:303-311 (2005)). The enzyme in Lactococcus lactis has been characterized on a variety of branched and linear substrates including 2-oxobutanoate, 2-oxohexanoate, 2-oxopentanoate, 3-methyl-2-oxobutanoate, 4-methyl-2-oxobutanoate and isocaproate (Smit et al., Appl Environ Microbiol 71:303-311 (2005)). The enzyme has been structurally characterized (Berg et al., Science. 318:1782-1786 (2007)). Sequence alignments between the Lactococcus lactis enzyme and the pyruvate decarboxylase of Zymomonas mobilus indicate that the catalytic and substrate recognition residues are nearly identical (Siegert et al., Protein Eng Des Sel 18:345-357 (2005)), so this enzyme would be a promising candidate for directed engineering. Decarboxylation of alpha-ketoglutarate by a BCKA was detected in Bacillus subtilis; however, this activity was low (5%) relative to activity on other branched-chain substrates (Oku and Kaneda, J Biol Chem. 263:18386-18396 (1988)) and the gene encoding this enzyme has not been identified to date. Additional BCKA gene candidates can be identified by homology to the Lactococcus lactis protein sequence. Many of the high-scoring BLASTp hits to this enzyme are annotated as indolepyruvate decarboxylases (EC 4.1.1.74). Indolepyruvate decarboxylase (IPDA) is an enzyme that catalyzes the decarboxylation of indolepyruvate to indoleacetaldehyde in plants and plant bacteria. Recombinant branched chain alpha-keto acid decarboxylase enzymes derived from the E1 subunits of the mitochondrial branched-chain keto acid dehydrogenase complex from Homo sapiens and Bos taurus have been cloned and functionally expressed in E. coli (Davie et al., J. Biol. Chem. 267:16601-16606 (1992); Wynn et al., J. Biol. Chem. 267:12400-12403 (1992); Wynn et al., J. Biol. Chem. 267:1881-1887 (1992)). In these studies, the authors found that co-expression of chaperonins GroEL and GroES enhanced the specific activity of the decarboxylase by 500-fold (Wynn et al., J. Biol. Chem. 267:12400-12403 (1992)). These enzymes are composed of two alpha and two beta subunits.
  • Protein GenBank ID GI Number Organism
    kdcA AAS49166.1 44921617 Lactococcus lactis
    kdc P9WG37.1 614088617 Mycobacterium tuberculosis
    BcG H37Rv
    BCKDHB NP_898871.1 34101272 Homo sapiens
    BCKDHA NP_000700.1 11386135 Homo sapiens
    BCKDHB P21839 115502434 Bos taurus
    BCKDHA P11178 129030 Bos taurus
  • The acetolactate synthase from Bacillus subtilis (AlsS), which naturally catalyzes the condensation of two molecules of pyruvate to form 2-acetolactate, is also able to catalyze the decarboxylation of 2-ketoisovalerate like KDC both in vivo and in vitro [PMID=19684168].
    • 4.2.1 Dehydratase
  • The dehydration of 2-hydroxypent-3-enoyl-CoA (1P), 3-hydroxypent-4-enoyl-CoA (2O), 3,4-dihydroxypentanoyl-CoA (3H), 4-hydroxy pent-2-enoyl-CoA (3M) and 5-hydroxypent-2-enoyl-CoA (2N) can be catalyzed by a special class of oxygen-sensitive enzymes that dehydrate 2-hydroxyacyl-CoA derivatives by a radical-mechanism (Buckel and Golding, Annu. Rev. Microbiol. 60:27-49 (2006); Buckel et al., Curr. Opin. Chem. Biol. 8:462-467 (2004); Buckel et al., Biol. Chem. 386:951-959 (2005); Kim et al., FEBS J. 272:550-561 (2005); Kim et al., FEMS Microbiol. Rev. 28:455-468 (2004); Zhang et al., Microbiology 145 (Pt 9):2323-2334 (1999)). One example of such an enzyme is the lactyl-CoA dehydratase from Clostridium propionicum, which catalyzes the dehydration of lactoyl-CoA to form acryloyl-CoA (Kuchta and Abeles, J. Biol. Chem. 260:13181-13189 (1985); Hofmeister and Buckel, Eur. J. Biochem. 206:547-552 (1992)). An additional example is 2-hydroxyglutaryl-CoA dehydratase encoded by hgdABC from Acidaminococcus fermentans (Mueller and Buckel, Eur. J. Biochem. 230:698-704 (1995); Schweiger et al., Eur. J. Biochem. 169:441-448 (1987)). Purification of the dehydratase from A. fermentans yielded two components, A and D. Component A (HgdC) acts as an activator or initiator of dehydration. Component D is the actual dehydratase and is encoded by HgdAB. Variations of this enzyme have been found in Clostridum symbiosum and Fusobacterium nucleatum. Component A, the activator, from A. fermentans is active with the actual dehydratse (component D) from C. symbiosum and is reported to have a specific activity of 60 per second, as compared to 10 per second with the component D from A. fermentans. Yet another example is the 2-hydroxyisocaproyl-CoA dehydratase from Clostridium difficile catalyzed by hadBC and activated by hadI (Darley et al., FEBS J. 272:550-61 (2005)). The sequence of the complete C. propionicium lactoyl-CoA dehydratase is not yet listed in publicly available databases. However, the sequence of the beta-subunit corresponds to the GenBank accession number AJ276553 (Selmer et al, Eur J Biochem, 269:372-80 (2002)). The dehydratase from Clostridium sporogens that dehydrates phenyllactyl-CoA to cinnamoyl-CoA is also a potential candidate for this step. This enzyme is composed of three subunits, one of which is a CoA transferase. The first step comprises of a CoA transfer from cinnamoyl-CoA to phenyllactate leading to the formation of phenyllactyl-CoA and cinnamate. The product cinnamate is released. The dehydratase then converts phenyllactyl-CoA into cinnamoyl-CoA. FldA is the CoA transferase and FldBC are related to the alpha and beta subunits of the dehydratase, component D, from A. fermentans.
  • GenBank
    Gene Accession No. GI No. Organism
    hgdA P11569 296439332 Acidaminococcus fermentans
    hgdB P11570 296439333 Acidaminococcus fermentans
    hgdC P11568 2506909 Acidaminococcus fermentans
    hgdA AAD31676.1 4883832 Clostridum symbiosum
    hgdB AAD31677.1 4883833 Clostridum symbiosum
    hgdC AAD31675.1 4883831 Clostridum symbiosum
    hgdA EDK88042.1 148322792 Fusobacterium nucleatum
    hgdB EDK88043.1 148322793 Fusobacterium nucleatum
    hgdC EDK88041.1 148322791 Fusobacterium nucleatum
    FldB Q93AL9.1 75406928 Clostridium sporogens
    FldC Q93AL8.1 75406927 Clostridium sporogens
    hadB YP_001086863 126697966 Clostridium difficile
    hadC YP_001086864 126697967 Clostridium difficile
    hadI YP_001086862 126697965 Clostridium difficile
    lcdB AJ276553 7242547 Clostridium propionicum
  • Another dehydratase that can potentially conduct such a biotransformation is the enoyl-CoA hydratase (4.2.1.17) of Pseudomonas putida, encoded by ech that catalyzes the conversion of 3-hydroxybutyryl-CoA to crotonyl-CoA (Roberts et al., Arch. Microbiol 117:99-108 (1978)). This transformation is also catalyzed by the crt gene product of Clostridium acetobutylicum, the crt1 gene product of C. kluyveri, and other clostridial organisms Atsumi et al., Metab Eng 10:305-311 (2008); Boynton et al., J Bacteriol. 178:3015-3024 (1996); Hillmer et al., FEBS Lett. 21:351-354 (1972)). Additional enoyl-CoA hydratase candidates are phaA and phaB, of P. putida, and paaA and paaB from P. fluorescens (Olivera et al., Proc. Natl. Acad. Sci U.S.A 95:6419-6424 (1998)). The gene product of pimF in Rhodopseudomonas palustris is predicted to encode an enoyl-CoA hydratase that participates in pimeloyl-CoA degradation (Harrison et al., Microbiology 151:727-736 (2005)). Lastly, a number of Escherichia coli genes have been shown to demonstrate enoyl-CoA hydratase functionality including maoC (Park et al., J Bacteriol. 185:5391-5397 (2003)), paaF (Ismail et al., Eur. J Biochem. 270:3047-3054 (2003); Park et al., Appl. Biochem. Biotechnol 113-116:335-346 (2004); Park et al., Biotechnol Bioeng 86:681-686 (2004)) and paaG (Ismail et al., Eur. J Biochem. 270:3047-3054 (2003); Park and Lee, Appl. Biochem. Biotechnol 113-116:335-346 (2004); Park and Yup, Biotechnol Bioeng 86:681-686 (2004)).
  • GenBank
    Gene Accession No. GI No. Organism
    ech NP_745498.1 26990073 Pseudomonas putida
    crt NP_349318.1 15895969 Clostridium acetobutylicum
    crtl YP_001393856 153953091 Clostridium kluyveri
    phaA NP_745427.1 26990002 Pseudomonas putida KT2440
    phaB NP_745426.1 26990001 Pseudomonas putida KT2440
    paaA ABF82233.1 106636093 Pseudomonas fluorescens
    paaB ABF82234.1 106636094 Pseudomonas fluorescens
    maoC NP_415905.1 16129348 Escherichia coli
    paaF NP_415911.1 16129354 Escherichia coli
    paaG NP_415912.1 16129355 Escherichia coli
  • Alternatively, the E. coli gene products of fadA and fadB encode a multienzyme complex involved in fatty acid oxidation that exhibits enoyl-CoA hydratase activity (Yang et al., Biochemistry 30:6788-6795 (1991); Yang, J Bacteriol. 173:7405-7406 (1991); Nakahigashi et al., Nucleic Acids Res. 18:4937 (1990)). Knocking out a negative regulator encoded by fadR can be utilized to activate the fadB gene product (Sato et al., J Biosci. Bioeng 103:38-44 (2007)). The fadI and fadJ genes encode similar functions and are naturally expressed under anaerobic conditions (Campbell et al., Mol. Microbiol 47:793-805 (2003)).
  • Protein GenBank ID GI Number Organism
    fadA YP_026272.1 49176430 Escherichia coli
    fadB NP_418288.1 16131692 Escherichia coli
    fadI NP_416844.1 16130275 Escherichia coli
    fadJ NP_416843.1 16130274 Escherichia coli
    fadR NP_415705.1 16129150 Escherichia coli
  • The dehydration of crotyl alcohol (1M, 4H), methyl vinyl carbinol (1L, 2Q, 3F, 4G), 3-hydroxybutyraldehyde (1D), 4-hydroxy-2-oxovalerate (1E, 2C), 2-hydroxy pent3-enoate (1G), 4-hydroxypent-2-enoate (1V, 3K), 5-hydroxypent-2-enoate (2F), 3-hydroxypent-4-enoate (2R) and 3,4-dihydroxypentanoate (3D) can be catalyzed exemplary dehydratases including oleate hydratase, acyclic 1,2-hydratase, linalool dehydratase, dimethylmaleate hydratase, (S)-2-methylmalate dehydratase, fumarate hydratase, glycerol dehydratase and enoyl-CoA hydratase enzymes. Enzyme candidates are described below.
  • Alternatively, crotyl alcohol, 3-buten-2-ol and 3-buten-1-ol produced by culturing the non-naturally occurring microbial organisms described herein can be converted to butadiene by chemical dehydration in the presence of a chemical catalyst. For example see international patent application publication WO2012106516A1.
  • Oleate hydratases catalyze the reversible hydration of non-activated alkenes to their corresponding alcohols. Oleate hydratase enzymes disclosed in WO2011/076691 and WO 2008/119735 are incorporated by reference herein. Oleate hydratases from Elizabethkingia meningoseptica and Streptococcus pyogenes are encoded by ohyA and HMPREF0841_1446. Acyclic 1,2-hydratase enzymes (eg. EC 4.2.1.131) catalyze the dehydration of linear secondary alcohols, and are thus suitable candidates for the dehydration of MVC to butadiene. Exemplary 1,2-hydratase enzymes include carotenoid 1,2-hydratase, encoded by crtC of Rubrivivax gelatinosus (Steiger et al, Arch Biochem Biophys 414:51-8 (2003)), and lycopene 1,2-hydratase, encoded by cruF of Synechococcus sp. PCC 7002 and Gemmatimonas aurantiaca (Graham and Bryant, J Bacteriol 191: 2392-300 (2009); Takaichi et al, Microbiol 156: 756-63 (2010)). Dehydration of t-butyl alcohol, t-amyl alcohol and 2-methyl-MVC to isobutene, isoamylene and isoprene, respectively, is catalyzed by an unknown enzyme of Aquincola tertiaricarbonis L108 (Schaefer et al, AEM 78 (17): 6280-4 (2012); Schuster et al, J. Bacteriol 194:972-81 (2012); Schuster et al, J Bacteriol 194: 972-81 (2012)). Linalool dehydratase/isomerase of Castellaniella defragrans catalyzes the dehydration of linalool to myrcene (Brodkorb et al, J Biol Chem 285:30436-42 (2010)). Enzyme accession numbers and homologs are listed in the table below.
  • Protein GenBank ID GI Number Organism
    OhyA ACT54545.1 254031735 Elizabethkingia
    meningoseptica
    HMPREF0841_ ZP_07461147.1 306827879 Streptococcus
    1446 pyogenes ATCC
    10782
    P700755_13397 ZP_01252267.1 91215295 Psychroflexus torquis
    ATCC 700755
    RPB_2430 YP_486046.1 86749550 Rhodopseudomonas
    palustris
    CrtC AA093124.1 29893494 Rubrivivax gelatinosus
    CruF YP_001735274.1 170078636 Synechococcus sp.
    PCC 7002
    Ldi E1XUJ2.1 403399445 Castellaniella
    defragrans
    CGGC5_10771 ELA28661.1 429853596 Colletotrichum
    gloeosporioides Nara
    gc5
    UCRPA7_8726 EON95759.1 500251895 Togninia minima
    UCRPA7
    UCRNP2_8820 EOD44468.1 485917493 Neofusicoccum
    parvum UCRNP2
    STEHIDRAFT_ EIM80109.1 389738914 Stereum hirsutum
    68678 FP-91666 SS1
    NECHADRAFT_ XP_003040778.1 302883759 Nectria haematococca
    82460 mpVI 77-13-4
    AS9A_2751 YP_004493998.1 333920417 Amycolicicoccus
    subflavus DQS3-9A1
  • Dimethylmaleate hydratases catalyze the dehydration of (2R,3S)-2,3-dimethylmalate into dimethylmaleate (EC 4.2.1.85). Dimethylmaleate hydratases from Eubacterium barkeri are encoded by dmdA and dmdB (Alhapel et al., Proc Natl Acad Sci 103:12341-6 (2006)). (S)-2-methylmalate dehydratases catalyze the reversible hydration of mesaconate to citramalate (EC 4.2.1.34). An exemplary (S)-2-methylmalate dehydratase is encoded by LeuC and LeuD of Methanococcus jannaschii and has been shown to catalyze the second step in the leucine biosynthesis pathway (Lee et al, Biochem Biophys Res Commun 419(2):160-4 (2012)). Fumarate hydratases catalyze the interconversion of fumarate to malate (EC 4.2.1.2). Two classes of fumarate hydratases exist, where classification is dependent upon the arrangement of subunits and metal requirements. Exemplary class I and class II fumarate dehydratases are encoded by fumA and fumC of Escherichia coli (Tseng et al, J Bacteriol 183(2):461-7 (2001)), and fumC of Corynebacterium glutamicum (Genda et al, Biosci Biotechnol Biochem 70(5):1102-9 (2006)). Glycerol dehydratases (EC 4.2.1.30) catalyze the conversion of glycerol to 3-hydroxy-propionaldehyde and water. Exemplary glycerol dehydratases are encoded by dhaB of Klebsiella pneumoniae (Wang et al, Biotechnol J. 2(6):736-42, (2007)) and by dhaB of Citrobacter freundii (Seyfried et al, J Bacteriol. 178(19):5793-6 (1996)). Enoyl-CoA hydratases catalyze the hydration of the double bond between the second and third carbons on acyl-CoA (EC 4.2.1.17). Enoyl-CoA hydratases are involved in the breakdown of fatty acids. Exemplary enoyl-CoA hydratases are encoded by phaJ1 of Pseudomonas putida (Vo et al, J. Biosci. Bioeng. 106 (1), 95-98 (2008)), and paaF of Escherichia coli (Teufel et al, Proc Natl Acad Sci USA. 107(32):14390-5 (2010)).
  • Protein GenBank ID GI Number Organism
    dmdA ABC88408 86278276 Eubacterium barkeri
    dmdB ABC88409.1 86278277 Eubacterium barkeri
    LeuC 4KP1_A 635576713 Methanococcus jannaschii
    LeuD Q58673.1 3122345 Methanococcus jannaschii
    fumA NP_416129.1 16129570 Escherichia coli
    fumC NP_416128.1 16129569 Escherichia coli
    fumC BAB98403.1 21323777 Corynebacterium glutamicum
    phJl ABP99034.1 145967354 Pseudomonas putida
    paaF P76082.1 2494240 Escherichia coli
    dhaB YP_002236501.1 206579582 Klebsiella pneumoniae
    dhaB AAB48850.1 493087 Citrobacter freundii
    • Dehydratase and Vinylisomerase Activity (FIG. 1(Steps G, M) and FIG. 4 (Steps F, G and H))
  • Bifunctional enzymes with dehydratase and isomerase activities are suitable for dehydrating and rearranging alcohols to alkenes as shown in FIGS. 1-4. This type of enzyme is required to convert 2-hydroxypent-3-enoate to 2,4-pentadienoate (Step G of FIG. 1) and crotyl alcohol to butadiene (FIG. 1M and FIG. 4H). For example, transformation 1 G can be catalyzed by the isomerization of 2-hydroxypent-3-enoate to 4-hydroxypent-2-enoate, followed by a dehydration of 4-hydroxypent-2-enoate to 2,4-pentadienoate. An exemplary bifunctional enzyme with isomerase and dehydratase activities is the linalool dehydratase/isomerase of Castellaniella defragrans. This enzyme catalyzes the isomerization of geraniol to linalool and the dehydration of linalool to myrcene, reactants similar in structure to CrotOH, MVC, 2-hydroxypent-3-enoate, butadiene and 2,4-pentadienoate and is also active on crotyl alcohol (Brodkorb et al, J Biol Chem 285:30436-42 (2010)). Enzyme accession numbers and homologs are listed in the table below.
  • GI
    Protein GenBank ID Number Organism
    Ldi E1XUJ2.1 403399445 Castellaniella
    defragrans
    CGGC5_10771 ELA28661.1 429853596 Colletotrichum
    gloeosporioides
    Nara gc5
    UCRPA7_8726 EON95759.1 500251895 Togninia minima
    UCRPA7
    UCRNP2_8820 EOD44468.1 485917493 Neofusicoccum
    parvum
    UCRNP2
    STEHIDRAFT_ EIM80109.1 389738914 Stereum hirsutum
    68678 FP-91666 SS1
    NECHADRAFT_ XP_003040778.1 302883759 Nectria haematococca
    82460 mpVI 77-13-4
    AS9A_2751 YP_004493998.1 333920417 Amycolicicoccus
    subflavus DQS3-9A1
  • Alternatively, a fusion protein or protein conjugate can be generated using well know methods in the art to generate a bi-functional (dual-functional) enzyme having both the isomerase and dehydratase activities. The fusion protein or protein conjugate can include at least the active domains of the enzymes (or respective genes) of the isomerase and dehydratase reactions. For the first step, the conversion of CrotOH to 3-buten-2-ol or 2-hydroxypent-3-enoate to 2,4-pentadienoate, enzymatic conversion can be catalyzed by a CrotOH or 2-hydroxypent-3-enoate isomerase (classified as EC 5.3.3 and EC 5.4.4). A similar isomerization, the conversion of 2-methyl-MVC to 3-methyl-2-buten-1-ol, is catalyzed by cell extracts of Pseudomonas putida MB-1 (Malone et al, AEM 65 (6): 2622-30 (1999)). The extract may be used in vitro, or the protein or gene(s) associated with the isomerase activity can be isolated and used, even though they have not been identified to date. Alternatively, either or both steps can be done by chemical conversion, or by enzymatic conversion (in vivo or in vitro), or any combination. Crotyl alcohol can be converted to butadiene by chemical dehydration in the presence of a chemical catalyst. For example see international patent application publication WO2012106516A1.
  • Linalool dehydratase/isomerase, Genbank ID number 403399445, was cloned from Castellaniella defragrans 65Phen into a plasmid suitable for expression in E. coli, plasmid pZS*13S obtained from R. Lutz (Expressys, Germany) and are based on the pZ Expression System (Lutz, R. & Bujard, H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-12 regulatory elements. Nucleic Acids Res. 25, 1203-1210 (1997)).
  • E. coli variants were transformed with the expression plasmid and selected and maintained using antibiotic selection with carbenicillin. The day before the experiment, 1 mL overnight cultures in LB-antibiotic were inoculated and grown with a breathable seal in 24 well plate at 37° C. Overnight cultures were seeded at OD600=0.05 into fresh 2 mL M9+4% glucose+antibiotic+IPTG+10 mM crotyl alcohol into 10 ml screw-cap bottles. Bottles were incubated for 48 hours at 37° C. and 1,3-butadiene production was validated by headspace analysis by GC-MS. In the absence of enzyme, no production of 1,3-butadiene was observed.
    • 5.3.3. Vinylisomerase
  • Vinylisomerase catalyzes the conversion of Crotyl alcohol to MVC (1K, 4F), 2-hydroxypent-3-enoate to 4-hydroxypent-2-enoate (1U), 3-hydroxypent-4-enoyl-CoA to 5-hydroxypent-2-enoyl-CoA (2M) and 2-hydroxypent-4-enoate to 5-hydroxypent-2-enoate (2E). The conversion of 3-buten-1-ol to crotyl alcohol can be carried out by vinyl-isomerases (see FIG. 2 Step S). The conversion of 3-buten-2-ol to crotyl alcohol can be carried out by vinyl-isomerases (see FIG. 1 Step W and FIG. 3 Step O). The enzymes described below naturally possess such activity or can be engineered to exhibit this activity. Intramolecular oxidoreductases that shift carbon-carbon double bonds from one position to another are found in the EC 5.3.3 enzyme class. The table below lists several useful enzymes in EC 5.3.3.
  • Enzyme
    Commission
    No. Enzyme Name
    5.3.3.2 Isopentenyl-diphosphate Δ-isomerase
    5.3.3.3 Vinylacetyl-CoA Δ-isomerase
    5.3.3.6 Methylitaconate Δ-isomerase
    5.3.3.7 Aconitate Δ-isomerase
    5.3.3.8 Dodecenoyl-CoA isomerase
    5.3.3.10 5-carboxymethyl-2-hydroxymuconate Δ-isomerase
    5.3.3.11 Isopiperitenone Δ-isomerase
    5.3.3.13 Polyenoic fatty acid isomerase
    5.3.3.14 Trans-2-decenoyl-[acyl-carrier-protein] isomerase
  • Particularly useful enzymes include isopentenyl-diphosphate Δ-isomerase, vinylacetyl-CoA Δ-isomerase and methylitaconate Δ-isomerase. Enzymes candidates are described below. Also useful is the vinylisomerase activity of linalool dehydratase.
  • Isopentenyl diphophaste isomerases catalyze the interconversion of isopentenyl diphosphate and dimethylallyl diphosphate, but can also catalyze the interconversion of CrotOH to MC (EC 5.3.3.2). Exemplary isopentenyl diphophaste isomerases are encoded by IDI-2 of Thermus thermophilus (Sharma et al, Biochemistry 49(29): 6228-6233 (2010)), idi of Xanthophyllomyces dendrorhous and idi of Haematococcus pluvialis (Kajiwara et al, Biochem J 324(Pt 2): 421-426 (1997)). Crystal structures have been determined for the isopentenyl diphophaste isomerases from Escherichia coli (Durbecq et al, EMBO J 20(7): 1530-1537 (2001)) and from Methanocaldococcus jannaschii (Hoshino et al, Acta Crystallogr Sect F Struct Biol Cryst Commun 67(Pt 1): 101-103 (2011)). Enzyme accession numbers and homologs are listed in the table below.
  • Protein GenBank ID GI Number Organism
    IDI-2 YP_006050 46255138 Thermus thermophilus
    IDI NP_247857 15668172 Methanocaldococcus jannaschii
    DSM 2661
    IDI NP_417365.1 16130791 Escherichia coli
    IDI AB019035.1 3790385 Xanthophyllomyces dendrorhous
    IDI BAA33978.1 3790384 Haematococcus pluvialis
    IPI XP 003063615 303289255 Micromonas pusilla CCMP1545
  • Vinylacetyl-CoA Δ-isomerases catalyze the conversion of vinylacetyl-CoA to crotonyl-CoA (EC 5.3.3.3). Exemplary vinylacetyl-CoA Δ-isomerases are encoded by AbfD of Clostridium kluyveri (Scherf et al, Arch Microbiol 161(3):239-45 (1994)), abfD of Clostridium aminobutyricum (Scherf et al, Eur J Biochem 215(2):421-9 (1993)), and Msed_1321 of Metallosphaera sedula (Auemik et al, Appl Environ Microbiol 74(3):682-92 (2008)).
  • Protein GenBank ID GI Number Organism
    AbfD YP_001396399 153955634 Clostridium kluyveri
    abfD P55792.3 84028213 Clostridium aminobutyricum
    Msed_1321 ABP95479.1 145702337 Metallosphaera sedula
  • Methylitaconate Δ-isomerases catalyze the isomerization of itaconate (methylenesuccinate) to citraconate (methylmaleate) (EC 5.3.3.6). An exemplary methylitaconate Δ-isomerase is encoded by mii from Eubacterium barkeri (Alhapel et al, Proc Natl Acad Sci USA 103(33):12341-6 (2006)) and the crystal structure of this 3-methylitaconate-delta-isomerase has been determined (Velarde et al, J Mol Biol 391(3):609-20 (2009)). Enzyme accession numbers and homologs are listed in the table below.
  • Protein GenBank ID GI Number Organism
    Mii Q0QLE6.1 122953534 Eubacterium barkeri
    WP_024729903 WP_024729903.1 639739165 Clostridiales bacterium
    WP_021167098 WP_021167098.1 544738199 Sporomusa ovata
    • 5.4.4 Alcohol Mutases
  • Alcohol mutases that catalyze the conversion of 2-hydroxypent-4-enoate to 5-hydroxypent-2-enoate (2E) and 2-hydroxypent-4-enoate to 3-hydroxypent-4-enoate (2H) are found in the EC 5.4.4 enzyme class, which include isomerases that transfer hydroxyl groups. Exemplary isomerase enzymes suitable for the conversion of 2-hydroxypent-4-enoate to 5-hydroxypent-2-enoate include isochorismate synthase (EC 5.4.4.2) and geraniol isosmerase (EC 5.4.4.4). Isochorismate synthase catalyzes the isomerization of chorismate to isochorismate and encodes for essential components of the respiratory chain. Exemplary isochorismate synthases are encoded by menF and dhbC of Bacillus subtilis (Rowland et al, J Bacteriol. 178(3):854-61 (1996)) and by menF of Escherichia coli (Daruwala et al, J Bacteriol. 179(10):3133-8 (1997)). Geraniol isomerase catalyzes the isomerization of (3S)-linalool to geraniol. Exemplary geraniol isomerase is encoded by Ldi of Castellaniella defragrans (Brodkorb et al, J Biol Chem 285:30436-42 (2010)). Enzyme accession numbers and homologs are listed in the table below.
  • Protein GenBank ID GI Number Organism
    menF NP_391077 16080250 Bacillus subtilis
    dhbC NP_391079 255767733 Bacillus subtilis
    menF NP_416768 90111411 Escherichia coli
    Ldi E1XUJ2.1 403399445 Castellaniella defragrans
    CGGC5_10771 ELA28661.1 429853596 Colletotrichum
    gloeosporioides Nara gc5
    UCRPA7_8726 EON95759.1 500251895 Togninia minima UCRPA7
    • 4.2.3 Alkenol Synthase
  • Alkenol synthase catalyzes the conversion of 2-butenyl-4-phosphate and 2-butenyl-4-diphosphate to 3-buten-2-ol or MVC (4D and 4E respectively). The enzymes described below naturally possess such activity or can be engineered to exhibit this activity. Particularly useful enzymes include methylbutenol (MBO) synthase, linalool synthase and nerolidol synthase. Enzyme candidates are described below and found within EC 4.2.3.
  • Methylbutenol synthase naturally catalyzes the conversion of dimethylallyl diphosphate to methylbutenol, but can also catalyze the synthesis of 3-buten-2-ol or MVC from 2-butenyl-4-phosphate and 2-butenyl-4-diphosphate. An exemplary methylbutenol synthase is encoded by Tps-MBO1 of Pinus sabiniana (Gray et al, J Biol Chem. 286(23):20582-90 (2011)). Linalool synthases catalyze the conversion of geranyl diphosphate to linalool. Exemplary R- (EC 4.2.3.26) and S-linalool synthases (EC 4.2.3.25) are encoded by AY083653 of Mentha citrata (Crowell et al, Arch Biochem Biophys., 405(1):112-21 (2002)) and by Lis of Clarkia breweri (Dudareva et al, Plant Cell. 8(7): 1137-1148 (1996)), respectively. (3S, 6E)-nerolidol synthase (EC 4.2.3.48) catalyze the conversion of farnesyl diphosphate to nerolidol. An exemplary (3S, 6E)-nerolidol synthase is encoded by MtTps3 of Medicago truncatula (Arimura et al, Planta. 227(2):453-64 (2008)). Enzyme accession numbers and homologs are listed in the table below.
  • Protein GenBank ID GI Number Organism
    Tps-MBO1 AEB53064 328834891 Pinus sabiniana
    Tps-MBO3 AFJ73583.1 387233228 Picea pungens
    AY083653 AY083653 22900831 Mentha citrata
    Lis Q96376 75251076 Clarkia breweri
    MtTps3 AAV36466 54634934 Medicago truncatula
    Pttps3 AEI52903 336318893 Populus trichocarpa
  • Butadiene Synthase (Monophosphate) or BDS
  • BDS (monophosphate) catalyzes the conversion of 2-butenyl-4-phosphate to 1,3-butadiene (Step 4J). BDS enzymes described above for Step C in the EC 4.2.3 enzyme class may possess such activity or can be engineered to exhibit this activity.
    • Example 2: Decarboxylation of 2,4-Pentadienoate to Butadiene by a Phenylacrylate Decarboxylase
  • PadA1 (GI number: 1165293) and OhbA1 (GI number: 188496963) encoding phenylacrylate decarboxylase from S. cerevisiae were codon optimized by DNA 2.0 and were cloned by DNA 2.0 into the following vectors suitable for expression in E. coli, pD424-NH and pD441-NH respectively (DNA 2.0 Inc.). The genes were tested for decarboxylation of 2,4-pentadienoate and the enzymatic reactions were carried out under the following conditions:
    • 100 mM Tris-HCL pH 7.2 10 mM KCL 10 mM NaCL 5 mM DTT 20 mM 2,4-Pentadienoate
  • 1.5 mg/ml lysate of E. coli DH5α cells containing decarboxylase from S. cerevisiae
  • The control reactions with lysate in the absence of substrate were conducted in parallel. 100 μL reactions were incubated overnight with shaking (175 rpm) at 25° C. in 1.5 ml gas-tight vials. Headspace GCMS analysis was carried out on a 7890A GC with 5975C inert MSD using a GS-GASPRO column, 30 m×0.32 mm (Agilent Technologies). Static headspace sample introduction was performed on a CombiPAL autosampler (CTC Analytics) following 2 min incubation at 45 C. The presence of 1,3-butadiene was evaluated and the enzymatic reaction product was identified by direct comparison with a standard of 1,3-butadiene (Sigma). GC/MS analysis showed the production of 1,3-butadiene from the enzymatic samples but not from the lysate alone controls.
  • While no butadiene formation was detected with the no substrate-control, butadiene was measured when 2,4-PD was added as a substrate.
    • Example 3: Demonstration of Acetyl-CoA Reductase (1A Ad 2A), 4-Hydroxy 2-Oxovalerate Aldolase (1B and 2B), 4-Hydroxy 2-Oxovalerate Decarboxylase (1C)
  • Genes expressing acetyl-CoA reductase (bphJ from Burkholderia xenovorans LB400, GI no: 520923), 4-hydroxy 2-oxovalerate aldolase (bphI from Burkholderia xenovorans LB400, GI no: 520924), 4-hydroxy 2-oxovalerate decarboxylase (kdc from Mycobacterium tuberculosis BcG H37Rv, GI no: 614088617), and alcohol dehydrogenase (yjgB from Chronobacter sakazakii, GI no: 387852894) were cloned into a plasmid suitable for expression in E. coli, plasmid pZA23S (kanamycin resistance marker, p15A origin of replication) obtained from R. Lutz (Expressys, Germany) and are based on the pZ Expression System (Lutz, R. & Bujard, H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-12 regulatory elements. Nucleic Acids Res. 25, 1203-1210 (1997)).
  • E. coli (MG1655 variants) cells were transformed with the expression plasmid and selected and maintained using antibiotic selection with Kanamycin. Cells were grown for 72 hours in LB media with kanamycin and IPTG at 37° C. then harvested by centrifugation. The formation of a 4-carbon diol derived from 3-hydroxybutyraldehyde using glucose as the carbon substrate was measured (data not shown) while the empty vector control did not make any 4-carbon diol.
    • Example 4. Production of Butadiene from Crotyl Diphosphate Via Butadiene Synthase
  • Isoprene synthase, E.C. 4.2.3.27, Genbank ID number 63108310, was cloned from Populus alba into a plasmid suitable for expression in E. coli, plasmid pZS*13S (Expressys, Germany).
  • E. coli (MG1655 variants) were transformed with the expression plasmid and selected and maintained using antibiotic selection with carbenicillin. Cells were grown in Terrific Broth with carbenicillin to an OD of 0.8 and then gene expression induced by IPTG addition then harvested by centrifugation. Lysis was performed using microfluidization at 0° C. Streptactin-tagged isoprene synthase was isolated from the cell lysate using Streptactin-Sepharose purification. Purified enzyme was tested for its ability to convert its native substrate, dimethylallyl diphosphate, into isoprene, and for its ability to convert crotyl diphosphate into 1, 3-butadiene, by incubating purified enzyme with each substrate in sealed screw-cap vials for a period of time before analysis of product in headspace of vial by GC-MS. Fidelity of purified enzyme was confirmed by detection of isoprene. Activity on crotyl diphosphate was confirmed by detection of butadiene. In the absence of enzyme, no butadiene was formed (data not shown).
    • Example 5. Demonstration of Crotyl Phosphate to Crotyl Phosphate Enzyme Activity
  • Isopentenyl phosphate kinase, E.C. 2.7.4.26, Genbank ID number 2621082, was cloned from Methanobacterium thermoautotrophicum gi12621082 into a plasmid suitable for expression in E. coli, plasmid pZS*13S obtained from R. Lutz (Expressys, Germany) and are based on the pZ Expression System (Lutz, R. & Bujard, H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 25, 1203-1210 (1997)).
  • E. coli (MG1655 variants) were transformed with the expression plasmid and selected and maintained using antibiotic selection with carbenicillin. Cells were grown in LB media with carbenicillin and IPTG at 37° C. then harvested by centrifugation. Lysis was performed using a chemical lysis procedure, and lysate the cooled to 4° C. Streptactin-tagged isopentenyl phosphate kinase was isolated from the cell lysate using Streptactin-Sepharose purification. Activity measurements on native substrate, isopentenyl phosphate, were performed to verify fidelity of the purified enzyme, using a pyruvate kinase-lactate dehydrogenase coupled assay to couple ADP formation from ATP to NADH oxidation. The same assay procedure was used to demonstrate robust activity on crotyl phosphate. In the absence of enzyme, no conversion of crotyl phosphate to crotyl diphosphate was observed (data not shown).
    • Example 6: CrotOH Isomerase Activity
  • Isopentenyl-diphosphate DELTA-isomerase (IPP isomerase), E.C. 5.3.3.2, Genbank ID number 3790386, was cloned from Xanthophyllomyces dendrorhous into a plasmid suitable for expression in E. coli, plasmid pZS*13S obtained from R. Lutz (Expressys, Germany) and are based on the pZ Expression System (Lutz, R. & Bujard, H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 25, 1203-1210 (1997)).
  • E. coli (MG1655 variants) were transformed with the expression plasmid and selected and maintained using antibiotic selection with carbenicillin. Cells were grown in LB media with carbenicillin and IPTG at 37° C. then harvested by centrifugation. Cell lysates containing the IPP isomerase from Xanthophyllomyces dendrorhous were generated by sonicating cells resuspended in 100 mM Tris, 50 mM KCl, 5 mM MgSO4, pH 7.2, and 5 mM DTT. 50 mM CrotOH was added to resulting cell lysate at 2.3 mg/ml total protein, and the reaction mixture incubated at 25° C. for 16 hours. The product of the isomerization reaction, MVC or 3-buten-2-ol, was detected at 1.2 mM by GCMS. In cell lysates devoid of the IPP isomerase, there was no detectable conversion of CrotOH to 3-buten-2-ol.
  • MVC or 3-buten-2-ol was measured using headspace analysis on an Agilent 7890A GC equipped with a CTC-PAL autosampler and a MSD (5975C). Samples were diluted 2-fold in 100% methanol to a total volume of 0.100 mL, and transferred into glass inserts in 1.5 mL GC vials. Samples were injected by a Combi PAL CTC autosampler operated in direct injection mode with an injection volume of 1.0 μL (split ratio 20:1) and 200° C. inlet temperature. Helium was used as a carrier gas, and the flow rate maintained at 1.28 mL/min. The oven temperature is initially held at 100° C. for 1 minute, then ramping 100° C./min to 230° C., for 3 minutes. The MVC or 3-buten-2-ol concentration in samples were calculated from calibration curves generated from diluted MVC standards analyzed under the same GCMS method.
  • The results of this example are presented in FIGS. 5 and 6 in the attached slide deck. Minimal media containing 1 mM MVC or 3-buten-2-ol without E. coli cells showed a peak at 3.24 minutes corresponding to MVC or 3-buten-2-ol. Cell lysates containing 50 mM CrotOH and the IPP isomerase from Xanthophyllomyces dendrorhous showed a peak at 3.24 minutes corresponding to MVC or 3-buten-2-ol (FIG. 6). In contrast, cell lysates devoid of the IPP isomerase show no detectable MVC or 3-buten-2-ol production (data not shown). These results demonstrate that cell lysates of E. coli harboring the IPP isomerase from Xanthophyllomyces dendrorhous, isomerize CrotOH to MC or 3-buten-2-ol.
    • Example 7: Crotyl Alcohol Dehydrogenase Activity
  • Alcohol dehydrogenase, Genbank ID number 407959257, was cloned from Synechocystis sp. PCC 6803 into a plasmid suitable for expression in E. coli, plasmid pZS*13S obtained from R. Lutz (Expressys, Germany) and are based on the pZ Expression System (Lutz, R. & Bujard, H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 25, 1203-1210 (1997)).
  • E. coli (MG1655 variants) were transformed with the expression plasmid and selected and maintained using antibiotic selection with carbenicillin. Cells were grown in LB media with carbenicillin and IPTG at 37° C. then harvested by centrifugation. Lysis was performed using a chemical lysis procedure, and lysate was then cooled to 4° C. Streptactin-tagged alcohol dehydrogenase was isolated from the cell lysate using Streptactin-Sepharose purification. Activity measurements assessing the NADPH-dependent reduction of crotonaldehyde to crotonol were performed. The alcohol dehydrogenase from Synechocystis sp. PCC 6803 was found to have a KM value of 0.16 mM and a Kcat of at least 36 s-1. In the absence of enzyme, no reduction of crotonaldehyde to crotonol was observed.

Claims (21)

1-23. (canceled)
24. A non-naturally occurring microbial organism having a pathway to butadiene, crotyl alcohol, 2,4-pentadienoate, or 3-buten-1-ol, said microbial organism comprising at least one exogenous nucleic acid encoding a pathway enzyme, the enzyme selected from the group consisting of (2E) 2-hydroxypent-4-enoate vinylisomerase, (2H) 2-hydroxypent-4-enoate mutase, and (2M) 3-hydroxypent-4-enoyl-CoA vinylisomerase.
25. The non-naturally occurring microorganism of claim 24, further comprising a nucleic acid encoding an enzyme selected from an alcohol dehydrogenase, an acyl-CoA reductase, a CoA transferase, a CoA hydrolase, a decarboxylase, an aldolase, a dehydratase, an isomerase, a CoA synthetase, an alcohol mutase, or combinations thereof.
26. The non-naturally occurring microorganism of claim 24, further comprising one or more nucleic acid(s) encoding an enzyme selected from (2A) acetyl-CoA reductase, (2B) 4-hydroxy 2-oxovalerate aldolase, (2C) 4-hydroxy 2-oxovalerate dehydratase, and (2D) 2-oxopent-4-enoate reductase.
27. The non-naturally occurring microorganism of claim 24, wherein the enzyme is (2E) 2-hydroxypent-4-enoate vinylisomerase.
28. The non-naturally occurring microorganism of claim 27, further comprising a nucleic acid encoding one or more enzyme(s) selected from (2P) 5-hydroxypent-2-enoate decarboxylase and (2Q) 3-buten-1-ol dehydratase or further comprising a nucleic acid encoding one or more enzyme(s) selected from (2P) 5-hydroxypent-2-enoate decarboxylase and (2S) vinylisomerase.
29. The non-naturally occurring microorganism of claim 27, further comprising a nucleic acid encoding one or more enzyme(s) selected from (2F) 5-hydroxypent-2-enoate dehydratase and (2G) 2,4-pentadienoate decarboxylase.
30. The non-naturally occurring microorganism of claim 24, wherein the enzyme is (2H) 2-hydroxypent-4-enoate mutase.
31. The non-naturally occurring microorganism of claim 30, further comprising a nucleic acid encoding one or more enzyme(s) selected from (2R) 3-hydroxypent-4-enoate dehydratase and (2G) 2,4-pentadienoate decarboxylase.
32. The non-naturally occurring microorganism of claim 30, further comprising a nucleic acid encoding (2K) 3-hydroxypent-4-enoate decarboxylase.
33. The non-naturally occurring microorganism of claim 30, further comprising a nucleic acid encoding one or more enzyme(s) selected from (2L) 3-hydroxypent-4-enoyl-CoA synthetase or transferase, (2M) 3-hydroxypent-4-enoyl-CoA vinylisomerase, (2 I 5-hydroxypent-2-enoyl-CoA synthetase, transferase or hydrolase (2F) 5-hydroxypent-2-enoate dehydratase, and (2G) 2,4-pentadienoate decarboxylase.
34. The non-naturally occurring microorganism of claim 30, further comprising a nucleic acid encoding one or more enzyme(s) selected from (2L) 3-hydroxypent-4-enoyl-CoA synthetase or transferase, (2M) 3-hydroxypent-4-enoyl-CoA vinylisomerase, (2N) 2,4-pentadienoyl-CoA synthetase, (2 J) 2,4-pentadienoyl-CoA synthetase, transferase or hydrolase, and (2G) 2,4-pentadienoate decarboxylase.
35. The non-naturally occurring microorganism of claim 30, further comprising a nucleic acid encoding one or more enzyme(s) selected from (2L) 3-hydroxypent-4-enoyl-CoA synthetase or transferase, (2O) 3-hydroxypent-4-enoyl-CoA dehydratase, (2 J) 2,4-pentadienoyl-CoA synthetase, transferase or hydrolase, and (2G) 2,4-pentadienoate decarboxylase.
36. The non-naturally occurring microbial organism of claim 24 having a pathway to convert pyruvate and acetyl-CoA to butadiene, crotyl alcohol, 2,4-pentadienoate, or 3-buten-1-ol.
37. The non-naturally occurring microorganism of claim 24 wherein any one or more nucleic acid(s) is an exogenous nucleic acid.
38. A method for the production of butadiene, crotyl alcohol, 2,4-pentadienoate, or 3-buten-1-ol, comprising culturing the non-naturally occurring microorganism of claim 24 under conditions to produce butadiene, crotyl alcohol, 2,4-pentadienoate, or 3-buten-1-ol.
39. The method for the production of butadiene, crotyl alcohol, 2,4-pentadienoate, or 3-buten-1-ol of claim 38, wherein the culturing comprises substantially anaerobic conditions.
40. A non-naturally occurring microbial organism having a pathway to butadiene, 2,4-pentadienoate, or 3-buten-2-ol, said microbial organism comprising at least one exogenous nucleic acid encoding a pathway enzyme, the enzyme selected from the group consisting of (3D) 3,4-dihydroxypentanoate dehydratase, (3E) 4-hydroxypent-2-enoate decarboxylase (3H) 3,4-dihydroxypentanoyl-CoA dehydratase, and (3J) 4-hydroxypent-2-enoyl-CoA transferase.
41-44. (canceled)
45. A non-naturally occurring microbial organism having a pathway to butadiene or 3-buten-2-ol, said microbial organism comprising at least one exogenous nucleic acid encoding a pathway enzyme, the enzyme selected from the group consisting of (4D) 3-buten-2-ol synthase, (4E) 3-buten-2-ol synthase, and (4F) crotyl alcohol isomerase.
46-72. (canceled)
US16/888,339 2014-07-03 2020-05-29 Microorganisms for producing 4c-5c compounds with unsaturation and methods related thereto Abandoned US20210002673A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US16/888,339 US20210002673A1 (en) 2014-07-03 2020-05-29 Microorganisms for producing 4c-5c compounds with unsaturation and methods related thereto

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201462020901P 2014-07-03 2014-07-03
US201462082747P 2014-11-21 2014-11-21
PCT/US2015/039037 WO2016004334A1 (en) 2014-07-03 2015-07-02 Microorganisms for producing 4c-5c compounds with unsaturation and methods related thereto
US201615323360A 2016-12-30 2016-12-30
US16/888,339 US20210002673A1 (en) 2014-07-03 2020-05-29 Microorganisms for producing 4c-5c compounds with unsaturation and methods related thereto

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2015/039037 Continuation WO2016004334A1 (en) 2014-07-03 2015-07-02 Microorganisms for producing 4c-5c compounds with unsaturation and methods related thereto
US15/323,360 Continuation US10669561B2 (en) 2014-07-03 2015-07-02 Microorganisms for producing 4C-5C compounds with unsaturation and methods related thereto

Publications (1)

Publication Number Publication Date
US20210002673A1 true US20210002673A1 (en) 2021-01-07

Family

ID=55020005

Family Applications (2)

Application Number Title Priority Date Filing Date
US15/323,360 Active 2036-01-18 US10669561B2 (en) 2014-07-03 2015-07-02 Microorganisms for producing 4C-5C compounds with unsaturation and methods related thereto
US16/888,339 Abandoned US20210002673A1 (en) 2014-07-03 2020-05-29 Microorganisms for producing 4c-5c compounds with unsaturation and methods related thereto

Family Applications Before (1)

Application Number Title Priority Date Filing Date
US15/323,360 Active 2036-01-18 US10669561B2 (en) 2014-07-03 2015-07-02 Microorganisms for producing 4C-5C compounds with unsaturation and methods related thereto

Country Status (3)

Country Link
US (2) US10669561B2 (en)
EP (2) EP3164495B1 (en)
WO (1) WO2016004334A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113122563A (en) * 2021-04-22 2021-07-16 洛阳华荣生物技术有限公司 Method for constructing R-3-aminobutyric acid production strain

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190012145A (en) 2016-03-29 2019-02-08 젤터, 인코포레이티드 Expression of proteins in Gram-negative bacteria with a ratio of peripheral cytoplasmic volume to cytoplasmic volume of 0.5: 1 to 10: 1
US11180541B2 (en) 2017-09-28 2021-11-23 Geltor, Inc. Recombinant collagen and elastin molecules and uses thereof
CN113355367B (en) * 2018-03-22 2024-03-26 浙江工业大学 Application of keto acid reductase in the synthesis of chiral aromatic 2-hydroxy acids
JP7777451B2 (en) 2019-04-12 2025-11-28 ジェルター， インコーポレイテッド Recombinant elastin and its production
CN112831427B (en) * 2021-01-20 2022-08-23 山东大学 Yarrowia lipolytica for high yield of beta-carotene and application thereof
CN116790698B (en) * 2023-06-21 2024-06-18 南京大学 Thialdehyde synthesis method based on oxidative decarboxylase and its application

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3090816A (en) 1963-05-21 Isomerization of alkylene oxide
US3090815A (en) 1963-05-21 Isomerization of alkylene oxide
US3542883A (en) 1966-08-10 1970-11-24 Costin Nenitescu Process for isomerization of 1,2-alkylene oxides
US5958745A (en) 1996-03-13 1999-09-28 Monsanto Company Methods of optimizing substrate pools and biosynthesis of poly-β-hydroxybutyrate-co-poly-β-hydroxyvalerate in bacteria and plants
WO2008119735A1 (en) 2007-04-02 2008-10-09 Georg-August-Universität Göttingen Method of producing hydroxy fatty acids
EP2017344A1 (en) 2007-07-20 2009-01-21 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO Production of itaconic acid
US7947483B2 (en) 2007-08-10 2011-05-24 Genomatica, Inc. Methods and organisms for the growth-coupled production of 1,4-butanediol
JP2011518564A (en) 2008-04-23 2011-06-30 ダニスコ・ユーエス・インク Isoprene synthase mutants for isoprene production by improved microorganisms
US9909146B2 (en) 2008-07-04 2018-03-06 Scientist Of Fortune S.A. Production of alkenes by enzymatic decarboxylation of 3-hydroxyalkanoic acids
WO2010031079A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Systems using cell culture for production of isoprene
JP5585098B2 (en) 2009-03-06 2014-09-10 日産自動車株式会社 Non-contact power supply apparatus and method
EP2336341A1 (en) 2009-12-21 2011-06-22 Philippe Marliere Method for producing an alkene comprising the step of converting an alcohol by an enzymatic dehydration step
RU2012138943A (en) 2010-02-12 2014-03-20 Джево, Инк. THE YEAST MICRO-ORGANISMS WITH THE REDUCED ACCUMULATION OF BY-PRODUCTS FOR THE IMPROVED PRODUCTION OF FUELS, CHEMICALS AND AMINO ACIDS
JP2013535203A (en) * 2010-07-26 2013-09-12 ジェノマティカ・インコーポレイテッド Microorganisms and methods for biosynthesis of aromatic, 2,4-pentadienoate and 1,3-butadiene
MX348334B (en) 2011-02-02 2017-06-07 Genomatica Inc Microorganisms and methods for the biosynthesis of butadiene.
US9663801B2 (en) 2011-06-17 2017-05-30 Invista North America S.A.R.L. Methods of producing four carbon molecules
SG11201400113XA (en) * 2011-08-19 2014-08-28 Genomatica Inc Microorganisms and methods for producing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol and related alcohols
CN104321434A (en) * 2011-12-02 2015-01-28 英威达技术有限责任公司 Methods for biosynthesizing 1,3butadiene
JP2024013009A (en) 2022-07-19 2024-01-31 清水建設株式会社 Generation system, learning device, generation device, generation method, and program

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113122563A (en) * 2021-04-22 2021-07-16 洛阳华荣生物技术有限公司 Method for constructing R-3-aminobutyric acid production strain

Also Published As

Publication number Publication date
EP3164495A4 (en) 2017-12-13
US20170166931A1 (en) 2017-06-15
EP4389904A3 (en) 2024-10-30
EP3164495B1 (en) 2024-02-28
EP4389904A2 (en) 2024-06-26
WO2016004334A1 (en) 2016-01-07
EP3164495A1 (en) 2017-05-10
US10669561B2 (en) 2020-06-02

Similar Documents

Publication Publication Date Title
US20230126921A1 (en) Non-natural microbial organisms with improved energetic efficiency
US20200385763A1 (en) Microorganisms and methods for producing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol and related alcohols
US20210002673A1 (en) Microorganisms for producing 4c-5c compounds with unsaturation and methods related thereto
EP2607340B1 (en) Microorganisms and methods for the biosynthesis of aromatics, 2,4-pentadienoate and 1,3-butadiene
US20210238609A1 (en) Microorganisms and methods for producing butadiene and related compounds by formate assimilation
US20220220511A1 (en) Microorganisms and methods for producing butadiene and related compounds by formate assimilation
AU2013202930B2 (en) Microorganisms and methods for producing 1,3-butanediol and related alcohols

Legal Events

Date Code Title Description
STPP Information on status: patent application and granting procedure in general

Free format text: DOCKETED NEW CASE - READY FOR EXAMINATION

STPP Information on status: patent application and granting procedure in general

Free format text: NON FINAL ACTION MAILED

STPP Information on status: patent application and granting procedure in general

Free format text: RESPONSE TO NON-FINAL OFFICE ACTION ENTERED AND FORWARDED TO EXAMINER

STPP Information on status: patent application and granting procedure in general

Free format text: NON FINAL ACTION MAILED

STPP Information on status: patent application and granting procedure in general

Free format text: RESPONSE TO NON-FINAL OFFICE ACTION ENTERED AND FORWARDED TO EXAMINER

STPP Information on status: patent application and granting procedure in general

Free format text: FINAL REJECTION MAILED

STCB Information on status: application discontinuation

Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION