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US20150352112A1 - Voriconazole inclusion complexes - Google Patents

Voriconazole inclusion complexes Download PDF

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Publication number
US20150352112A1
US20150352112A1 US14/760,260 US201414760260A US2015352112A1 US 20150352112 A1 US20150352112 A1 US 20150352112A1 US 201414760260 A US201414760260 A US 201414760260A US 2015352112 A1 US2015352112 A1 US 2015352112A1
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Prior art keywords
cyclodextrin
voriconazole
hydroxypropyl
pharmaceutical formulation
stabilized pharmaceutical
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US14/760,260
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Anita Bevetek Mocnik
Ivona Jasprica
Sasa Grubesic
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Xellia Pharmaceuticals ApS
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Xellia Pharmaceuticals ApS
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Assigned to XELLIA PHARMACEUTICALS APS reassignment XELLIA PHARMACEUTICALS APS ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: GRUBESIC, Sasa, JASPRICA, Ivona, MOCNIK, ANITA BEVETEK
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/506—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim not condensed and containing further heterocyclic rings
    • A61K47/48969—
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6949—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit inclusion complexes, e.g. clathrates, cavitates or fullerenes
    • A61K47/6951—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit inclusion complexes, e.g. clathrates, cavitates or fullerenes using cyclodextrin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/08—Solutions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
    • C08B37/0012—Cyclodextrin [CD], e.g. cycle with 6 units (alpha), with 7 units (beta) and with 8 units (gamma), large-ring cyclodextrin or cycloamylose with 9 units or more; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
    • C08B37/0012—Cyclodextrin [CD], e.g. cycle with 6 units (alpha), with 7 units (beta) and with 8 units (gamma), large-ring cyclodextrin or cycloamylose with 9 units or more; Derivatives thereof
    • C08B37/0015—Inclusion compounds, i.e. host-guest compounds, e.g. polyrotaxanes
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
    • C08L5/16—Cyclodextrin; Derivatives thereof

Definitions

  • Voriconazole is a triazole antifungal agent with structural formula:
  • Voriconazole is designated chemically as (2R,3S)-2-(2,4-difluorophenyl)-3-(5-fluoro-4-pyrimidinyl)-1-(1H-1,2,4triazol-1-yl)-2-butanol with an empirical formula of C 16 H 14 F 3 N 5 O and a molecular weight of 349.3.
  • Voriconazole contains a triazole functionality and is a single diastereomer with two reported pKa values of 4.98 and 12.0. Voriconazole is a weak base; it is not hygroscopic and is classified as having a low solubility (very slightly soluble in water), and being a high permeability compound (BCS class II).
  • Voriconazole is a chiral antifungal agent belonging to the class of triazole antifungals.
  • the reference drug product is marketed in Europe and US under the trade name Vfend® by Pfizer.
  • the drug is indicated for the treatment of invasive aspergillosis ( Aspergillus fumigatus ) and esophageal candidiasis ( Candidia albicans ), as well as infections caused by Scedosporium apiospermum and Fusarium spp.
  • Voriconazole is not a stable molecule—it degrades in water, it is susceptible to oxidative degradation and decomposes in acidic and basic media. Photodegradation occurs under severe light stress conditions and it degrades greatly under elevated temperature.
  • Specified degradation products of voriconazole are identified as:
  • Voriconazole is semi-polar in nature which means that it is generally not solubilized by conventional means such as oils, surfactants or water miscible co-solvents.
  • the voriconazole drug substance is a white to off white powder in solid state.
  • U.S. Pat. No. 6,632,803 provides a pharmaceutical formulation comprising voriconazole, or a pharmaceutically acceptable salt thereof, and a sulfobutylether ⁇ -cyclodextrin.
  • EP 2 018 866 discloses a process for improving the solubility of voriconazole in aqueous solutions comprising voriconazole and a beta-cyclodextrin.
  • WO 2012/171561 discloses a process for improving the stability of voriconazole in pharmaceutical compositions comprising voriconazole and a beta-cyclodextrin, wherein the said composition comprises a stabilizing amount of lactose.
  • Voriconazole lyophilized formulation contains 200 mg of voriconazole and is intended for reconstitution with Water for Injection to obtain a solution containing 10 mg/mL of Voriconazole and 160 mg/mL of sulfobutyl-ether beta-cyclodextrin. The resultant solution is further diluted prior to administration as an intravenous infusion.
  • the present invention provides new voriconazole formulations comprising 2-hydroxypropyl- ⁇ -cyclodextrins.
  • the invention is inter alia based on the discovery that formulations comprising voriconazole are stabilized when formulated using 2-hydroxy- ⁇ -propyl-cyclodextrin within a certain range of molar substitution.
  • a stabilized pharmaceutical formulation comprising voriconazole and a substituted ⁇ -cyclodextrin characterized by a molar substitution of the ⁇ -cyclodextrin by hydroxypropyl groups of more than 0.8, provided that the formulation does not comprise lactose.
  • the pharmaceutical formulation of the present invention comprises substituted ⁇ -cyclodextrins wherein the substituent on the ⁇ -cyclodextrin is a 2-hydroxypropyl group.
  • the molar substitution of the 2-hydroxypropyl ⁇ -cyclodextrin is 0.8-1.1, preferably 0.8-1.0. According to yet another embodiment, the molar substitution of the 2-hydroxypropyl ⁇ -cyclodextrin is 0.9.
  • the said stabilized pharmaceutical formulation has a pH in the range of about 4-7.
  • the pharmaceutical formulation according to the present invention further comprises a pH adjusting agent.
  • the pharmaceutical formulation according to the present invention further comprises an acidification agent.
  • the pharmaceutical formulation according to the present invention further comprises organic carboxylic acids.
  • the pharmaceutical formulation according to the present invention further comprises citrate, acetate, tartrate and/or succinate buffers.
  • the pharmaceutical formulation according to the present invention further comprises citric, acetic, tartaric and/or succinic acids.
  • the pharmaceutical formulation according to the above mentioned invention is further lyophilized.
  • the present pharmaceutical formulation may comprise about 4-10% w/w voriconazole in solid state, such as about 6% w/w voriconazole in solid state.
  • the present pharmaceutical formulation may comprise about 90-96% w/w ⁇ -cyclodextrin in solid state, such as e.g. about 94% w/w ⁇ -cyclodextrin in solid state.
  • the formulation comprises voriconazole and 2-hydroxypropyl- ⁇ -cyclodextrin in a molar ratio of up to 1:5.
  • the formulation comprises voriconazole and 2-hydroxypropyl- ⁇ -cyclodextrin in a molar ratio of 1:2 to 1:5
  • the formulation comprises voriconazole and 2-hydroxypropyl- ⁇ -cyclodextrin in a molar ratio of 1:3.6
  • the formulation in solid state comprises voriconazole and 2-hydroxypropyl- ⁇ -cyclodextrin in a weight ratio of 1:22 to 1:10.
  • the formulation in solid state comprises voriconazole and 2-hydroxypropyl- ⁇ -cyclodextrin in a weight ratio of 1:18 to 1:14.
  • the formulation in solid state comprises voriconazole and 2-hydroxypropyl- ⁇ -cyclodextrin in a weight ratio of 1:16.
  • a reconstituted formulation consisting of a solution comprising a stabilized pharmaceutical formulation according to the present invention being dissolved in a diluent suitable for injection or intravenous infusion.
  • the reconstituted formulation according to the present invention may comprise 1-20 mg/ml voriconazole.
  • the reconstituted formulation according to the present invention may comprise 50-300 mg/ml 2-hydroxypropyl- ⁇ -cyclodextrin.
  • a stabilized pharmaceutical formulation consisting of:
  • the pH of said formulation is preferably within the range of about 4-7.
  • a stabilizing effect is achieved by keeping the pH within a certain range when formulating voriconazole formulation comprising substituted ⁇ -cyclodextrin.
  • a stabilized pharmaceutical comprising voriconazole and a substituted ⁇ -cyclodextrin, wherein the compositions has pH of 4-7 when dissolved in a suitable diluent.
  • the pharmaceutical formulation according to the present invention further comprises a pH adjusting agent.
  • the pharmaceutical formulation according to the present invention further comprises an acidification agent.
  • the pharmaceutical formulation according to the present invention further comprises organic carboxylic acids.
  • the pharmaceutical formulation according to the present invention further comprises citrate, acetate, tartrate and/or succinate buffers.
  • said formulation based on the findings of the stabilizing effect of the pH, may be further lyophilized and reconstituted similar to the other embodiments of the present invention.
  • the present applications furthermore provide a method for stabilizing a composition comprising voriconazole, wherein the method comprises the steps of:
  • the prepared composition does not comprise lactose.
  • the present application provides the use of a substituted ⁇ -cyclodextrin having a molar substitution of the ⁇ -cyclodextrin by hydroxypropyl groups of more than 0.8 as an agent for stabilization of a composition comprising voriconazole.
  • Voriconazole is only sparingly soluble in water. Without being bound by theory, it is believed that 2-hydroxypropyl-substituted ⁇ -cyclodextrin can form inclusion complex with voriconazole and thus increase its aqueous solubility. Further, such voriconazole complexes are more stable in aqueous media than voriconazole itself.
  • Cyclodextrins are a group of structurally related natural products formed by bacterial digestion of cellulose. These cyclic oligosaccharides consist of ( ⁇ -1,4)-linked ⁇ -D-glucopyranose units and contain a somewhat lipophilic central cavity and a hydrophilic outer surface. Due to the chair conformation of the glucopyranose units, the cyclodextrins are shaped like a truncated cone rather than perfect cylinders. The hydroxyl functions are orientated to the cone exterior with the primary hydroxyl groups of the sugar residues at the narrow edge of the cone and the secondary hydroxyl groups at the wider edge.
  • the central cavity is lined by the skeletal carbons and ethereal oxygens of the glucose residues, which gives it a lipophilic character.
  • the polarity of the cavity has been estimated to be similar to that of an aqueous ethanolic solution.
  • the natural ⁇ -, ⁇ - and ⁇ -cyclodextrin consist of six, seven, and eight glucopyranose units, respectively.
  • Cyclodextrin derivatives of pharmaceutical interest include the hydroxypropyl derivatives of ⁇ - and ⁇ -cyclodextrin, the randomly methylated ⁇ -cyclodextrin, sulfobutylether ⁇ -cyclodextrin, and the so-called branched cyclodextrins such as glucosyl- ⁇ -cyclodextrin.
  • cyclodextrins are able to form inclusion complexes with many drugs by taking up a drug molecule or more frequently some lipophilic moiety of the molecule, into the central cavity. No covalent bonds are formed or broken during the complex formation and drug molecules in the complex are in rapid equilibrium with free molecules in the solution.
  • the driving forces for the complex formation include release of enthalpy-rich water molecules from the cavity, electrostatic interactions, van der Waals interactions, hydrophobic interactions, hydrogen bonding, and release of conformational strain and charge-transfer interactions.
  • cyclodextrins have mainly been used as complexing agents to increase aqueous solubility of poorly soluble drugs, and to increase their bioavailability and stability.
  • CDs and their complexes are hydrophilic, their aqueous solubility can be rather limited, especially in the case of ⁇ CD. This is thought to be due to relatively strong binding of the CD molecules in the crystal state (i.e. relatively high crystal lattice energy). Random substitution of hydroxyl groups, even by hydrophobic moieties such as methoxy functions, will result in dramatic improvements in their solubility. Moreover, some derivatives, such as 2-hydroxypropyl (HP ⁇ CD and HP ⁇ CD) and sulfobutylether (SBE ⁇ CD), possess improved toxicological profiles in comparison to their parent CDs.
  • HP ⁇ CD and HP ⁇ CD 2-hydroxypropyl
  • SBE ⁇ CD sulfobutylether
  • the Degree of Substitution of cyclodextrin is defined as the average number of substituted hydroxyl groups per glucopyranose unit of CD ring. Since the number of reactive hydroxyls per mole of glucopyranose unit is 3, the maximum numbers of substituents possible for ⁇ -, ⁇ -, and ⁇ -CDs are 18, 21, and 24, respectively.
  • molar substitution Another term used to describe cyclodextrin substitution is molar substitution (MS).
  • MS molar substitution
  • the “molar substitution of the ⁇ -cyclodextrin by hydroxypropyl groups” means the average number of hydroxypropyl substituents attached to each glucopyranose unit in the cyclodextrin.
  • composition as used in this specification, means any formulation intended for therapeutic or prophylactic treatment.
  • Pharmaceutical formulations according to the present invention can be in solid or liquid state.
  • ⁇ -cyclodextrin as used in this specification, means any cyclodextrin comprising 7 ( ⁇ -1,4)-linked ⁇ -D-glucopyranose units, e.g.
  • Hydroxypropyl- ⁇ -cyclodextrin as used in this specification, means any ⁇ -cyclodextrin monomer comprising at least one hydroxypropyl substituent attached to a hydroxyl group on the cyclodextrin. Hydroxypropyl- ⁇ -cyclodextrin is abbreviated as HP ⁇ CD.
  • hydroxypropyl substituent as used in this specification is meant to embrace several different substituents including:
  • 2-hydroxypropyl- ⁇ -cyclodextrin as used in this specification, means any ⁇ -cyclodextrin monomer comprising at least one 2-hydroxypropyl substituent attached to a hydroxyl group on the ⁇ -cyclodextrin.
  • 2-hydroxypropyl- ⁇ -cyclodextrin is abbreviated 2-HP ⁇ CD.
  • 2-hydroxypropyl- ⁇ -cyclodextrin could be represented as on the following figure:
  • pH of 3-8 is meant to include pH 8.0, pH 7.5, 7.0, pH 6.5, pH 3.0. Further included is any pH between any of these; e.g. pH 5.23, pH 3.35, pH 7.39 etc.
  • pH of 4-7 is meant to include pH 4.0, pH 5.5, pH 6.0, pH 7.0. Further included is any pH between any of these; e.g. pH 4.23, pH 5.35, pH 5.39 etc.
  • Water for injection as used herein is substantially pure and sterile water, e.g. water purified by distillation or a purification process that is equivalent or superior to distillation in the removal of chemicals and microorganisms.
  • Ultratunization water is water with conductivity below 0.055 ⁇ S and pH in the range from 5.0 to 7.0 and is used in the following examples as a substitute for “water for injection”.
  • “Stabilizing effect” as used herein is a reduction of the level of impurities in solid or liquid formulations formulated according to the parameters described in the claims, in comparison to the formulations which are not manufactured within the same parameters.
  • “Stabilized pharmaceutical formulations” are formulations in which voriconazole degrades in lower extent when formulations are exposed to stability testing at elevated temperature in comparison to the non-stabilized formulations in which decomposition of voriconazole is greater under the same stability testing conditions.
  • the voriconazole formulations were prepared in the following manner: first 2-hydroxypropyl- ⁇ -cyclodextrin was dissolved in the appropriate vehicle in concentration of 160 mg/mL and then voriconazole was added to the solution in a concentration of 10 mg/mL. After preparation, liquid formulations were filled in vials and subsequently lyophilized.
  • composition was prepared according to the above described procedure and as a vehicle ultrapure water was used. Stability of finished product was tested at 40° C./75% RH in inverted position (taken as a worst case) during 1 month. The results are shown in Table 1.
  • compositions were prepared according to the above described procedure, and as a vehicle different buffers were used.
  • the stability of finished products was tested at 40° C./75% RH in inverted position (taken as a worst case) during 1 month. The results are shown in Table 2.
  • compositions were prepared according to the above described procedure, and as a vehicle ultrapure water was used. Stability of finished products was tested at 40° C./75% RH in inverted position (taken as a worst case) during 2 weeks. The results are shown in Table 3.
  • composition was prepared according to the above described procedure, and as a vehicle ultrapure water was used. Stability of finished product was tested at 40° C./75% RH in inverted position (taken as a worst case) during 3 months. The results are shown in Table 4.
  • compositions were prepared according to the above described procedure, and as a vehicle different buffers were used.
  • the stability of finished products was tested at 40° C./75% RH in inverted position (taken as a worst case) during 3 months. The results are shown in Table 5.
  • composition was prepared according to the above described procedure, and as a vehicle ultrapure water was used. pH of formulation was adjusted to 8.5 using 0.1M NaOH prior batch volume make up. The stability of finished product was tested at 40° C./75% RH in inverted position (taken as a worst case) during 2 weeks. The results are shown in Table 6.

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Abstract

The present invention relates to new voriconazole formulations comprising 2-hydroxypropyl-□-cyclodextrins and the preparation thereof.

Description

    BACKGROUND
  • Voriconazole is a triazole antifungal agent with structural formula:
  • Figure US20150352112A1-20151210-C00001
  • Voriconazole is designated chemically as (2R,3S)-2-(2,4-difluorophenyl)-3-(5-fluoro-4-pyrimidinyl)-1-(1H-1,2,4triazol-1-yl)-2-butanol with an empirical formula of C16H14F3N5O and a molecular weight of 349.3.
  • Voriconazole contains a triazole functionality and is a single diastereomer with two reported pKa values of 4.98 and 12.0. Voriconazole is a weak base; it is not hygroscopic and is classified as having a low solubility (very slightly soluble in water), and being a high permeability compound (BCS class II).
  • Voriconazole is a chiral antifungal agent belonging to the class of triazole antifungals. The reference drug product is marketed in Europe and US under the trade name Vfend® by Pfizer. The drug is indicated for the treatment of invasive aspergillosis (Aspergillus fumigatus) and esophageal candidiasis (Candidia albicans), as well as infections caused by Scedosporium apiospermum and Fusarium spp.
  • The prior art implies that Voriconazole is not a stable molecule—it degrades in water, it is susceptible to oxidative degradation and decomposes in acidic and basic media. Photodegradation occurs under severe light stress conditions and it degrades greatly under elevated temperature.
  • Specified degradation products of voriconazole are identified as:
  • Figure US20150352112A1-20151210-C00002
    • 1-(2,4-difluorophenyl)-2-(1H-1,2,4-triazol-1-yl) ethanone,
  • Figure US20150352112A1-20151210-C00003
    • 4-ethyl-5-fluoropyrimidine,
  • Figure US20150352112A1-20151210-C00004
    • ((2RS,3SR)-2-(2,4-difluorophenyl)-3-(pyrimidin-4-yl)-1-(1H-1,2,4-triazol-1-yl)butan-2-ol) and enantiomer, and
  • Figure US20150352112A1-20151210-C00005
    • (2RS,3RS)-2-(2,4-difluorophenyl)-3-(5-fluoropyrimidin-4-yl)-1-(1H-1,2,4-triazol-1-yl)butan-2-ol (voriconazole enantiomer).
  • Voriconazole is semi-polar in nature which means that it is generally not solubilized by conventional means such as oils, surfactants or water miscible co-solvents. The voriconazole drug substance is a white to off white powder in solid state.
  • In order to obtain water a soluble formulation of voriconazole that is suitable for administration by intravenous infusion, the solubility of the active compound has to be enhanced. 2-hydroxypropyl-β-cyclodextrin has been used for that purpose and the basis for its usage is the ability of 2-hydroxypropyl-β-cyclodextrin to interact with poorly water-soluble voriconazole resulting in an increase in its apparent water solubility. The mechanism for this solubilization is rooted in the ability of 2-hydroxypropyl-β-cyclodextrin to form non-covalent dynamic inclusion complexes in a solution, in which the guest and host molecules are in dynamic equilibrium with the complex.
  • U.S. Pat. No. 6,632,803 provides a pharmaceutical formulation comprising voriconazole, or a pharmaceutically acceptable salt thereof, and a sulfobutylether β-cyclodextrin.
  • EP 2 018 866 discloses a process for improving the solubility of voriconazole in aqueous solutions comprising voriconazole and a beta-cyclodextrin.
  • WO 2012/171561 discloses a process for improving the stability of voriconazole in pharmaceutical compositions comprising voriconazole and a beta-cyclodextrin, wherein the said composition comprises a stabilizing amount of lactose.
  • The marketed Voriconazole lyophilized formulation (Vfend®) contains 200 mg of voriconazole and is intended for reconstitution with Water for Injection to obtain a solution containing 10 mg/mL of Voriconazole and 160 mg/mL of sulfobutyl-ether beta-cyclodextrin. The resultant solution is further diluted prior to administration as an intravenous infusion.
    • SUMMARY OF THE INVENTION
  • The present invention provides new voriconazole formulations comprising 2-hydroxypropyl-β-cyclodextrins. The invention is inter alia based on the discovery that formulations comprising voriconazole are stabilized when formulated using 2-hydroxy-β-propyl-cyclodextrin within a certain range of molar substitution.
  • According to one embodiment of the present invention, a stabilized pharmaceutical formulation is thus provided comprising voriconazole and a substituted β-cyclodextrin characterized by a molar substitution of the β-cyclodextrin by hydroxypropyl groups of more than 0.8, provided that the formulation does not comprise lactose.
  • According to another embodiment, the pharmaceutical formulation of the present invention comprises substituted β-cyclodextrins wherein the substituent on the β-cyclodextrin is a 2-hydroxypropyl group.
  • In one embodiment of the present invention, the molar substitution of the 2-hydroxypropyl β-cyclodextrin is 0.8-1.1, preferably 0.8-1.0. According to yet another embodiment, the molar substitution of the 2-hydroxypropyl β-cyclodextrin is 0.9.
  • According to another embodiment of the present invention, the said stabilized pharmaceutical formulation has a pH in the range of about 4-7.
  • According to a further embodiment, the pharmaceutical formulation according to the present invention further comprises a pH adjusting agent.
  • According to a further embodiment, the pharmaceutical formulation according to the present invention further comprises an acidification agent.
  • According to a further embodiment, the pharmaceutical formulation according to the present invention further comprises organic carboxylic acids.
  • According to a further embodiment, the pharmaceutical formulation according to the present invention further comprises citrate, acetate, tartrate and/or succinate buffers.
  • According to a further embodiment, the pharmaceutical formulation according to the present invention further comprises citric, acetic, tartaric and/or succinic acids.
  • According to yet another aspect of the present invention, the pharmaceutical formulation according to the above mentioned invention is further lyophilized.
  • The present pharmaceutical formulation may comprise about 4-10% w/w voriconazole in solid state, such as about 6% w/w voriconazole in solid state.
  • Furthermore, the present pharmaceutical formulation may comprise about 90-96% w/w β-cyclodextrin in solid state, such as e.g. about 94% w/w β-cyclodextrin in solid state.
  • According to yet another aspect of the present invention, the formulation comprises voriconazole and 2-hydroxypropyl-β-cyclodextrin in a molar ratio of up to 1:5.
  • According to a another embodiment of the present invention, the formulation comprises voriconazole and 2-hydroxypropyl-β-cyclodextrin in a molar ratio of 1:2 to 1:5
  • According to the most preferred embodiment of the present invention, the formulation comprises voriconazole and 2-hydroxypropyl-β-cyclodextrin in a molar ratio of 1:3.6
  • According to a preferred embodiment of the present invention, the formulation in solid state comprises voriconazole and 2-hydroxypropyl-β-cyclodextrin in a weight ratio of 1:22 to 1:10.
  • According to a preferred embodiment of the present invention, the formulation in solid state comprises voriconazole and 2-hydroxypropyl-β-cyclodextrin in a weight ratio of 1:18 to 1:14.
  • According to a preferred embodiment of the present invention, the formulation in solid state comprises voriconazole and 2-hydroxypropyl-β-cyclodextrin in a weight ratio of 1:16.
  • According to another aspect of the present invention, a reconstituted formulation is provided consisting of a solution comprising a stabilized pharmaceutical formulation according to the present invention being dissolved in a diluent suitable for injection or intravenous infusion.
  • The reconstituted formulation according to the present invention may comprise 1-20 mg/ml voriconazole.
  • The reconstituted formulation according to the present invention may comprise 50-300 mg/ml 2-hydroxypropyl-β-cyclodextrin.
  • According to yet another aspect of the present invention, a stabilized pharmaceutical formulation is provided, consisting of:
      • i. voriconazole;
      • ii. a substituted β-cyclodextrin characterized by a molar substitution of the β-cyclodextrin by 2-hydroxypropyl groups of more than 0.8;
      • iii. optionally pH adjusting agents; and
      • iv. optionally pharmaceutically acceptable diluents or solvents.
  • The pH of said formulation is preferably within the range of about 4-7.
  • The present inventors have also found that a stabilizing effect is achieved by keeping the pH within a certain range when formulating voriconazole formulation comprising substituted β-cyclodextrin. Thus, according to yet another aspect of the present invention, a stabilized pharmaceutical is provided comprising voriconazole and a substituted β-cyclodextrin, wherein the compositions has pH of 4-7 when dissolved in a suitable diluent.
  • According to a further embodiment of this aspect, the pharmaceutical formulation according to the present invention further comprises a pH adjusting agent.
  • According to yet a further embodiment, the pharmaceutical formulation according to the present invention further comprises an acidification agent.
  • According to yet a further embodiment, the pharmaceutical formulation according to the present invention further comprises organic carboxylic acids.
  • According to yet a further embodiment, the pharmaceutical formulation according to the present invention further comprises citrate, acetate, tartrate and/or succinate buffers.
  • According to yet a further embodiment, the pharmaceutical formulation according to the present invention further comprises citric, acetic, tartaric and/or succinic acids.
  • It is to be understood that said formulation, based on the findings of the stabilizing effect of the pH, may be further lyophilized and reconstituted similar to the other embodiments of the present invention.
  • The present applications furthermore provide a method for stabilizing a composition comprising voriconazole, wherein the method comprises the steps of:
      • a. providing an aqueous solution of 2-hydroxypropyl-β-cyclodextrin having a molar substitution of the β-cyclodextrin by hydroxypropyl groups of more than 0.8;
      • b. adding voriconazole;
      • c. optionally adjusting the pH; and
      • d. optionally lyophilizing the obtained stabilized composition.
  • According to one embodiment of the present method, the prepared composition does not comprise lactose.
  • Finally, the present application provides the use of a substituted β-cyclodextrin having a molar substitution of the β-cyclodextrin by hydroxypropyl groups of more than 0.8 as an agent for stabilization of a composition comprising voriconazole.
    • DETAILED DESCRIPTION OF THE INVENTION
  • Voriconazole is only sparingly soluble in water. Without being bound by theory, it is believed that 2-hydroxypropyl-substituted β-cyclodextrin can form inclusion complex with voriconazole and thus increase its aqueous solubility. Further, such voriconazole complexes are more stable in aqueous media than voriconazole itself.
  • Furthermore, data from the literature imply that voriconazole is unstable in alkaline media, where it degrades quickly especially when it is exposed to elevated temperatures. Available data also imply that pH of the media can have an impact on the stability of voriconazole formulations.
  • Cyclodextrins are a group of structurally related natural products formed by bacterial digestion of cellulose. These cyclic oligosaccharides consist of (α-1,4)-linked α-D-glucopyranose units and contain a somewhat lipophilic central cavity and a hydrophilic outer surface. Due to the chair conformation of the glucopyranose units, the cyclodextrins are shaped like a truncated cone rather than perfect cylinders. The hydroxyl functions are orientated to the cone exterior with the primary hydroxyl groups of the sugar residues at the narrow edge of the cone and the secondary hydroxyl groups at the wider edge. The central cavity is lined by the skeletal carbons and ethereal oxygens of the glucose residues, which gives it a lipophilic character. The polarity of the cavity has been estimated to be similar to that of an aqueous ethanolic solution. The natural α-, β- and γ-cyclodextrin consist of six, seven, and eight glucopyranose units, respectively. Cyclodextrin derivatives of pharmaceutical interest include the hydroxypropyl derivatives of β- and γ-cyclodextrin, the randomly methylated β-cyclodextrin, sulfobutylether β-cyclodextrin, and the so-called branched cyclodextrins such as glucosyl-β-cyclodextrin.
  • In aqueous solutions cyclodextrins are able to form inclusion complexes with many drugs by taking up a drug molecule or more frequently some lipophilic moiety of the molecule, into the central cavity. No covalent bonds are formed or broken during the complex formation and drug molecules in the complex are in rapid equilibrium with free molecules in the solution. The driving forces for the complex formation include release of enthalpy-rich water molecules from the cavity, electrostatic interactions, van der Waals interactions, hydrophobic interactions, hydrogen bonding, and release of conformational strain and charge-transfer interactions.
  • In the pharmaceutical industry cyclodextrins have mainly been used as complexing agents to increase aqueous solubility of poorly soluble drugs, and to increase their bioavailability and stability.
  • Although the natural CDs and their complexes are hydrophilic, their aqueous solubility can be rather limited, especially in the case of βCD. This is thought to be due to relatively strong binding of the CD molecules in the crystal state (i.e. relatively high crystal lattice energy). Random substitution of hydroxyl groups, even by hydrophobic moieties such as methoxy functions, will result in dramatic improvements in their solubility. Moreover, some derivatives, such as 2-hydroxypropyl (HPβCD and HPγCD) and sulfobutylether (SBEβCD), possess improved toxicological profiles in comparison to their parent CDs.
  • The Degree of Substitution of cyclodextrin (DS) is defined as the average number of substituted hydroxyl groups per glucopyranose unit of CD ring. Since the number of reactive hydroxyls per mole of glucopyranose unit is 3, the maximum numbers of substituents possible for α-, β-, and γ-CDs are 18, 21, and 24, respectively.
  • Another term used to describe cyclodextrin substitution is molar substitution (MS). This term, as used in this specification, describes the average number of moles of the substituting agent, e.g, hydroxypropyl, per mole of glucopyranose. For example when a hydroxypropyl-β-cyclodextrin has DS=14, the MS is 14/7 or 2. Thus, the “molar substitution of the β-cyclodextrin by hydroxypropyl groups”, as used in this specification, means the average number of hydroxypropyl substituents attached to each glucopyranose unit in the cyclodextrin.
  • Pharmaceutical formulation, as used in this specification, means any formulation intended for therapeutic or prophylactic treatment. Pharmaceutical formulations according to the present invention can be in solid or liquid state.
  • β-cyclodextrin, as used in this specification, means any cyclodextrin comprising 7 (α-1,4)-linked α-D-glucopyranose units, e.g.
  • Figure US20150352112A1-20151210-C00006
  • Hydroxypropyl-β-cyclodextrin, as used in this specification, means any β-cyclodextrin monomer comprising at least one hydroxypropyl substituent attached to a hydroxyl group on the cyclodextrin. Hydroxypropyl-β-cyclodextrin is abbreviated as HPβCD.
  • The hydroxypropyl substituent as used in this specification is meant to embrace several different substituents including:
  • —CH2—CH2—CH2OH
  • —CH2—CHOH—CH3
  • —CHOH—CH2—CH3
  • —CHOH—CHOH—CH3
  • —CHOH—CH2—CH2OH
  • —CH2—CHOH—CH2OH
  • —CHOH—CHOH—CH2OH
  • One example of a hydroxypropyl-β-cyclodextrin could be represented:
  • Figure US20150352112A1-20151210-C00007
  • wherein R═-CH2—CH2—CH2OH
  • 2-hydroxypropyl-β-cyclodextrin, as used in this specification, means any β-cyclodextrin monomer comprising at least one 2-hydroxypropyl substituent attached to a hydroxyl group on the β-cyclodextrin. 2-hydroxypropyl-β-cyclodextrin is abbreviated 2-HPβCD. One example of a 2-hydroxypropyl-β-cyclodextrin could be represented as on the following figure:
  • Figure US20150352112A1-20151210-C00008
  • wherein R═-CH2—CHOH—CH3—
  • Whenever pH is mentioned in respect of a formulation according to the present invention, e.g. in respect of formulation having a pH within a specified range, it is to be understood that the formulation is in liquid form if not otherwise stated.
  • “pH of 3-8” is meant to include pH 8.0, pH 7.5, 7.0, pH 6.5, pH 3.0. Further included is any pH between any of these; e.g. pH 5.23, pH 3.35, pH 7.39 etc.
  • “pH of 4-7” is meant to include pH 4.0, pH 5.5, pH 6.0, pH 7.0. Further included is any pH between any of these; e.g. pH 4.23, pH 5.35, pH 5.39 etc.
  • “Water for injection” as used herein is substantially pure and sterile water, e.g. water purified by distillation or a purification process that is equivalent or superior to distillation in the removal of chemicals and microorganisms. “Ultrapure water” is water with conductivity below 0.055 μS and pH in the range from 5.0 to 7.0 and is used in the following examples as a substitute for “water for injection”.
  • “Stabilizing effect” as used herein is a reduction of the level of impurities in solid or liquid formulations formulated according to the parameters described in the claims, in comparison to the formulations which are not manufactured within the same parameters.
  • “Stabilized pharmaceutical formulations” are formulations in which voriconazole degrades in lower extent when formulations are exposed to stability testing at elevated temperature in comparison to the non-stabilized formulations in which decomposition of voriconazole is greater under the same stability testing conditions.
    • EXAMPLES
  • The voriconazole formulations were prepared in the following manner: first 2-hydroxypropyl-β-cyclodextrin was dissolved in the appropriate vehicle in concentration of 160 mg/mL and then voriconazole was added to the solution in a concentration of 10 mg/mL. After preparation, liquid formulations were filled in vials and subsequently lyophilized.
  • The following hydroxypropyl beta cyclodextrins were used for preparation of voriconazole formulations:
  • 1. 2-hydroxypropyl-β-cyclodextrin with molar substitution=0.65
  • 2. 2-hydroxypropyl-β-cyclodextrin with molar substitution=0.63
  • 3. 2-hydroxypropyl-β-cyclodextrin with molar substitution=0.87
  • All formulations were analyzed immediately after lyophilization and then subjected to stability testing at the elevated temperature (40° C.±2° C./75%±5% RH). Analyses of formulations were done at defined time points and during the storage finished product vials were kept in inverted position.
  • Except pH, content of impurities was analyzed at each specified time point using validated HPLC methods.
  • The results of the study and conclusions are presented in text and Tables that follow.
    • Example 1
  • The composition was prepared according to the above described procedure and as a vehicle ultrapure water was used. Stability of finished product was tested at 40° C./75% RH in inverted position (taken as a worst case) during 1 month. The results are shown in Table 1.
  • TABLE 1
    Impurity profile and respective pH values of Voriconazole and
    2-hydroxypropyl-β-cyclodextrin formulation prepared using
    2-hydroxypropyl-β-cyclodextrin of molar substitution equal
    to 0.65 (ultrapure water was used as solvent).
    Formulation:
    Voriconazole and 2-hydroxy-
    propyl-β-cyclodextrin
    formulation (MS of 2HPβCD =
    0.65) in Ultrapure water
    Storage Condition:
    2 weeks 1 month
    40° C./ 40° C./
    75% RH 75% RH
    TESTS START IP IP
    pH 8.9  8.6 8.6
    Related substance (%)
    1-(2,4-difluorophenyl)-2-(1H- 0.08 1.2 1.8
    1,2,4-triazol-1-yl) ethanone
    4-ethyl-5-fluoropyrimidine 0.07 1.2 1.9
    ((2RS,3SR)-2-(2,4- <LOQ <LOQ <LOQ
    difluorophenyl)-3-(pyrimidin-4-
    yl)-1-(1H-1,2,4-triazol-1-
    yl)butan-2-ol)
    (2RS,3RS)-2-(2,4- <LOQ  0.30  0.45
    Difluorophenyl)-3-(5-
    fluoropyrimidin-4-yl)-1-(1H-
    1,2,4-triazol-1-yl)butan-2-ol
    Total Impurities 0.20 2.6 4.2
    Total impurities = sum of specified and unspecified impurities
  • From above presented results it can be seen that found level of impurities after two weeks of storage at 40° C./75% RH was rather high. Obtained pH values measured in all samples was in the range from 8.6 to 8.9, which implies that pH of formulation higher than 8 destabilizes the active compound.
    • Example 2
  • The compositions were prepared according to the above described procedure, and as a vehicle different buffers were used. The stability of finished products was tested at 40° C./75% RH in inverted position (taken as a worst case) during 1 month. The results are shown in Table 2.
  • TABLE 2
    Impurity profiles and respective pH values of voriconazole and 2-hydroxypropyl-β-cyclodextrin
    formulations prepared using 2-hydroxypropyl-β-cyclodextrin of molar substitution equal to 0.65
    dissolved in different buffers with pH value adjusted in the range from 3.8 to 7.2 subjected
    to stability testing at elevated temperature.
    Formulation:
    Voriconazole and
    2-hydroxypropyl-β- Voriconazole and 2- Voriconazole and 2-
    cyclodextrin hydroxypropyl-β- hydroxypropyl-β-
    formulation (MS of cyclodextrin formulation cyclodextrin formulation
    2-HPβCD = 0.65) in (MS of 2-HPβCD = 0.65) in (MS of 2-HPβCD = 0.65)
    Citrate buffer with Citrate buffer with pH in Citrate buffer with pH
    pH adjusted to 7.2 adjusted to 5.5 adjusted to 3.8
    Storage Condition:
    2 2 1 2 1
    TESTS START weeks* START weeks* month** START weeks* month**
    pH 7.4 7.3 5.7 5.7 5.8 4.1 4.1 4.1
    Related substances
    (%)
    1-(2,4-difluorophenyl)- 0.06 1.8 <LOQ 0.19 0.43 <LOQ 0.20 0.46
    2-(1H-1,2,4-triazol-1-
    yl) ethanone
    4-ethyl-5- 0.06 1.9 <LOQ 0.19 0.40 <LOQ 0.21 0.42
    fluoropyrimidine
    ((2RS,3SR)-2-(2,4- <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.05
    difluorophenyl)-3-
    (pyrimidin-4-yl)-1-
    (1H-1,2,4-triazol-1-
    yl)butan-2-ol)
    (2RS,3RS)-2-(2,4- <LOQ 0.49 <LOQ 0.05 0.11 <LOQ <LOQ 0.07
    Difluorophenyl)-3-(5-
    fluoropyrimidin-4-yl)-
    1-(1H-1,2,4-triazol-1-
    yl)butan-2-ol
    Total Impurities 0.17 4.2 0.05 0.47 0.98 <LOQ 0.45 1.0
    Formulation:
    Voriconazole and 2-
    Voriconazole and 2- hydroxypropyl-β-
    hydroxypropyl-β- cyclodextrin
    cyclodextrin formulation formulation (MS of
    (MS of 2-HPβCD = 0.65) in 2-HPβCD = 0.65) in
    Succinate buffer with pH Tartarate buffer
    adjusted to 4.0 with pH adjusted to 4.8
    Storage Condition:
    2 1 2 1
    TESTS START weeks* month** START weeks* month**
    pH 4.3 4.3 4.3 5.0 5.0 5.0
    Related substances
    (%)
    1-(2,4-difluorophenyl)- <LOQ 0.17 0.40 <LOQ 0.16 0.37
    2-(1H-1,2,4-triazol-1-
    yl) ethanone
    4-ethyl-5- <LOQ 0.18 0.37 <LOQ 0.17 0.35
    fluoropyrimidine
    ((2RS,3SR)-2-(2,4- <LOQ <LOQ <LOQ <LOQ <LOQ 0.05
    difluorophenyl)-3-
    (pyrimidin-4-yl)-1-
    (1H-1,2,4-triazol-1-
    yl)butan-2-ol)
    (2RS,3RS)-2-(2,4- <LOQ <LOQ 0.06 <LOQ <LOQ 0.09
    Difluorophenyl)-3-(5-
    fluoropyrimidin-4-yl)-
    1-(1H-1,2,4-triazol-1-
    yl)butan-2-ol
    Total Impurities <LOQ 0.40 0.90 <LOQ 0.44 0.91
    Total impurities = sum of specified and unspecified impurities
  • From obtained results in could be concluded that found level of impurities in formulations with pH adjusted in the range from 3.8 to 5.5 are approximately four times lower than in the voriconazole formulation that was prepared using 2-hydroxypropyl-β-cyclodextrin of molar substitution equal to 0.65 dissolved only in ultrapure water (for comparison please see Table 1). Formulation prepared with citrate buffer and pH adjusted to 7.2 showed significant degradation of active compound when exposed to elevated temperature, implying that formulation is destabilized in slightly alkaline media
    • Example 3
  • The compositions were prepared according to the above described procedure, and as a vehicle ultrapure water was used. Stability of finished products was tested at 40° C./75% RH in inverted position (taken as a worst case) during 2 weeks. The results are shown in Table 3.
  • TABLE 3
    Impurity profiles and respective pH values of Voriconazole and
    2-hydroxypropyl-β-cyclodextrin formulations prepared using
    2-hydroxypropyl-β-cyclodextrin of molar substitution equal
    to 0.87 or 0.63 dissolved in ultrapure water and subjected to
    stability testing at elevated temperature.
    Formulation:
    Voriconazole and Voriconazole and
    2-hydroxypropyl- 2-hydroxypropyl-
    β-cyclodextrin β-cyclodextrin
    formulation (MS of formulation (MS of
    2-HPβCD = 0.87) in 2-HPβCD = 0.63) in
    Ultrapure water Ultrapure water
    Storage Condition:
    2 weeks 2 weeks
    40° C./ 40° C./
    75% RH 75% RH
    TESTS START IP START IP
    pH 6.9 7.2  7.1 7.3 
    Related substances (%)
    1-(2,4-difluorophenyl)-2-(1H- <LOQ 0.22 <LOQ 0.43
    1,2,4-triazol-1-yl) ethanone
    4-ethyl-5-fluoropyrimidine <LOQ 0.20 <LOQ 0.39
    ((2RS,3SR)-2-(2,4- <LOQ <LOQ <LOQ <LOQ
    difluorophenyl)-3-(pyrimidin-
    4-yl)-1-(1H-1,2,4-triazol-1-
    yl)butan-2-ol)
    (2RS,3RS)-2-(2,4- <LOQ 0.05 <LOQ 0.11
    Difluorophenyl)-3-(5-
    fluoropyrimidin-4-yl)-1-(1H-
    1,2,4-triazol-1-yl)butan-2-ol
    Total Impurities <LOQ 0.47 <LOQ 0.93
    Total impurities = sum of specified and unspecified impurities
  • Obtained results imply that formulation with 2-HPβCD with higher MS is more stable than the formulation with lower MS, as the level of impurities is twice higher in formulation containing 2-HPβCD with lower molar substitution.
    • Example 4
  • The composition was prepared according to the above described procedure, and as a vehicle ultrapure water was used. Stability of finished product was tested at 40° C./75% RH in inverted position (taken as a worst case) during 3 months. The results are shown in Table 4.
  • TABLE 4
    Impurity profiles and respective pH values of Voriconazole and
    2-hydroxypropyl-β-cyclodextrin formulation prepared using
    2-hydroxypropyl-β-cyclodextrin of molar substitution equal
    to 0.87dissolved in ultrapure water and subjected to stability
    testing at elevated temperature.
    Formulation:
    Voriconazole and 2-hydroxypropyl-β-
    cyclodextrin formulation (MS of
    2-HPβCD = 0.87) in Ultrapure water
    Storage Condition:
    1 M 3 M
    40° C./ 40° C./
    TESTS START 75% RH IP 75% RH IP
    pH 6.5  6.4  6.4 
    Related substances (%)
    1-(2,4-difluorophenyl)-2-(1H- <LOD 0.35 0.91
    1,2,4-triazol-1-yl) ethanone
    4-ethyl-5-fluoropyrimidine <LOD 0.36 0.65
    ((2RS,3 SR)-2-(2,4- <LOD <LOQ <LOQ
    difluorophenyl)-3-(pyrimidin-4-
    yl)-1-(1H-1,2,4-triazol-1-
    yl)butan-2-ol)
    (2RS,3RS)-2-(2,4- <LOQ 0.10 0.15
    Difluorophenyl)-3-(5-
    fluoropyrimidin-4-yl)-1-(1H-
    1,2,4-triazol-1-yl)butan-2-ol
    Total Impurities 0.05 0.87 1.8 
    Total impurities = sum of specified and unspecified impurities
    • Example 5
  • The compositions were prepared according to the above described procedure, and as a vehicle different buffers were used. The stability of finished products was tested at 40° C./75% RH in inverted position (taken as a worst case) during 3 months. The results are shown in Table 5.
  • TABLE 5
    Impurity profiles and respective pH values of voriconazole and 2-hydroxypropyl-β-cyclodextrin formulations
    prepared using 2-hydroxypropyl-β-cyclodextrin of molar substitution equal to 0.63 dissolved in different
    buffers with pH value adjusted to 4.7 subjected to stability testing at elevated temperature.
    Formulation:
    Voriconazole and Voriconazole and Voriconazole and Voriconazole and
    2-hydroxypropyl- 2-hydroxypropyl- 2-hydroxypropyl- 2-hydroxypropyl-
    β-cyclodextrin β-cyclodextrin β-cyclodextrin β-cyclodextrin
    formulation (MS of formulation (MS of formulation (MS of formulation (MS of
    2-HPβCD = 0.63) 2-HPβCD = 0.63) 2-HPβCD = 0.63) 2-HPβCD = 0.63)
    in Citrate in Succinate in Tartrate in Acetate
    buffer with pH buffer with pH buffer with pH buffer with pH
    adjusted to 4.7 adjusted to 4.7 adjusted to 4.7 adjusted to 4.7
    Storage Condition:
    3 M 3 M 3 M 3 M
    40° C./75% 40° C./75% 40° C./75% 40° C./75%
    TESTS START RH IP START RH IP START RH IP START RH IP
    pH 4.9 5.0 4.9 5.0 5.0 5.0 5.1 5.1
    Related substances (%)
    1-(2,4-difluoro-phenyl)-2-(1H- <LOQ 1.20 <LOQ 1.28 <LOQ 0.96 <LOQ 1.6
    1,2,4-triazol-1-yl)-ethanone
    4-ethyl-5-fluoropyrimidine <LOQ 1.26 <LOQ 1.35 <LOQ 0.95 <LOQ 1.6
    ((2RS,3SR)-2-(2,4- <LOQ <LOQ <LOQ <LOQ 0.05 <LOQ 0.05 <LOQ
    difluorophenyl)-3-(pyrimidin-
    4-yl)-1-(1H-1,2,4-triazol-1-
    yl)butan-2-ol)
    (2RS,3RS)-2-(2,4- <LOQ 0.23 <LOQ 0.23 <LOQ 0.27 <LOQ 0.13
    Difluorophenyl)-3-(5-
    fluoropyrimidin-4-yl)-1-(1H-
    1,2,4-triazol-1-yl)butan-2-ol
    Total Impurities 0.07 2.7 0.07 2.9 0.10 2.2 0.05 3.4
    Total impurities = sum of specified and unspecified impurities
  • When comparing stability testing results presented in Tables 3 & 5 it can be seen that in buffered formulations (pH value in finished product equal to app. 5) containing 2-HPβCD with MS of 0.63, the level of impurities is significantly lower than in the voriconazole formulation containing 2-HPβCD with MS equal to 0.63 dissolved only in ultrapure water. The level of impurities in the later formulation is comparable to the impurities found in the formulation containing 2-HPβCD with MS equal to 0.87.
    • Example 6
  • The composition was prepared according to the above described procedure, and as a vehicle ultrapure water was used. pH of formulation was adjusted to 8.5 using 0.1M NaOH prior batch volume make up. The stability of finished product was tested at 40° C./75% RH in inverted position (taken as a worst case) during 2 weeks. The results are shown in Table 6.
  • TABLE 6
    Impurity profile and respective pH value of voriconazole and 2-
    hydroxypropyl-β-cyclodextrin formulation prepared using
    2-hydroxypropyl-β-cyclodextrin of molar substitution equal
    to 0.87 and pH value adjusted to 8.5 with 0.1M sodium
    hydroxide (NaOH) subjected to 2 weeks stability testing at
    elevated temperature.
    Formulation:
    Voriconazole and
    2-hydroxypropyl-β-
    cyclodextrin
    formulation
    (MS of 2-HPβCD =
    0.87) with pH
    adjusted to 8.5
    using 0.1M NaOH
    Storage conditions:
    2 weeks
    40° C./75%
    TESTS START RH IP
    pH 8.5  8.5 
    Related substances (%)
    1-(2,4-difluorophenyl)-2-(1H-1,2,4-triazol-1-yl)- 0.05 0.58
    ethanone
    4-ethyl-5-fluoropyrimidine 0.05 0.65
    ((2RS,3SR)-2-(2,4-difluorophenyl)-3-(pyrimidin- <LOQ <LOQ
    4-yl)-1-(1H-1,2,4-triazol-1-yl)butan-2-ol)
    (2RS,3RS)-2-(2,4-Difluorophenyl)-3-(5-fluoro- <LOQ 0.22
    pyrimidin-4-yl)-1-(1H-1,2,4-triazol-1-yl)butan-
    2-ol
    Total Impurities 0.10 1.5 
    Total impurities = sum of specified and unspecified impurities
  • When comparing above presented results to the results of 2 weeks of stability testing at 40° C./75% RH of the formulation prepared in Ultrapure water without additional pH adjustment (see Table 3 for comparison), it can be seen that found level of impurities in formulation with pH above 8.5 is approximately three times higher. This finding implies that voriconazole in the formulation with pH higher than 8 is less stable and degrades in greater extent.
  • Still, basic formulation (pH above 8.5) prepared with the 2-HPβCD with MS equal to 0.87 is more stable (level of impurities is twice lower) than the formulation with the similar pH formulated with the 2-HPβCD with MS equal to 0.63 (for comparison, see Table 1).

Claims (33)

1. A stabilized pharmaceutical formulation comprising voriconazole and a substituted β-cyclodextrin characterized by a molar substitution of the β-cyclodextrin by hydroxypropyl groups of more than 0.8, provided that the formulation does not comprise lactose.
2. The stabilized pharmaceutical formulation according to claim 1, wherein the substituent on the β-cyclodextrin is a 2-hydroxypropyl group.
3. The stabilized pharmaceutical formulation according to claim 1, wherein the molar substitution of the 2-hydroxypropyl β-cyclodextrin is 0.8-1.1.
4. The stabilized pharmaceutical formulation according to claim 3, wherein the molar substitution of the 2-hydroxypropyl β-cyclodextrin is 0.8-1.0.
5. The stabilized pharmaceutical formulation according to claim 4, wherein the molar substitution of the 2-hydroxypropyl β-cyclodextrin is 0.9.
6. The stabilized pharmaceutical formulation according to claim 1, having a pH in the range of about 4-7.
7. The stabilized pharmaceutical formulation according to claim 1, wherein the formulation further comprises a pH adjusting agent.
8. The stabilized pharmaceutical formulation according to claim 1, wherein the formulation further comprises an acidification agent.
9. The stabilized pharmaceutical formulation according to claim 8, wherein the formulation further comprises one or more organic carboxylic acids.
10. The stabilized pharmaceutical formulation according to claim 1, wherein the formulation further comprises citrate, acetate, tartrate and/or succinate buffers.
11. The stabilized pharmaceutical formulation according to claim 1, wherein the formulation further comprises citric, acetic, tartaric and/or succinic acids.
12. The stabilized pharmaceutical formulation according to claim 1, wherein the said formulation is further lyophilized.
13. The stabilized pharmaceutical formulation according to claim 12, comprising 4-10% w/w voriconazole in a solid state.
14. The stabilized pharmaceutical formulation according to claim 12, comprising 6% w/w voriconazole in a solid state.
15. The stabilized pharmaceutical formulation according to claim 12, comprising about 90-96% w/w β-cyclodextrin in a solid state.
16. The stabilized pharmaceutical formulation according to claim 15, comprising about 94% w/w β-cyclodextrin in the solid state.
17. The stabilized pharmaceutical formulation according to claim 1, wherein the said formulation comprises voriconazole and 2-hydroxypropyl-β-cyclodextrin in a molar ratio of up to 1:5.
18. The stabilized pharmaceutical formulation according to claim 1, wherein the said formulation comprises voriconazole and 2-hydroxypropyl-β-cyclodextrin in a molar ratio of 1:3.6.
19. The stabilized pharmaceutical formulation according to claim 1, wherein the said formulation comprises voriconazole and 2-hydroxypropyl-β-cyclodextrin in a weight ratio of 1:22 to 1:10.
20. The stabilized pharmaceutical formulation according to claim 19, wherein the formulation comprises voriconazole and 2-hydroxypropyl-β-cyclodextrin in a weight ratio of 1:18 to 1:14.
21. The stabilized pharmaceutical formulation according to claim 20, wherein the formulation comprises voriconazole and 2-hydroxypropyl-β-cyclodextrin in a weight ratio of 1:16
22. A reconstituted formulation, consisting of a solution of a formulation according to claim 1 dissolved in a diluent suitable for injection or intravenous infusion.
23. The reconstituted formulation according to claim 22, comprising 1-20 mg/ml voriconazole.
24. The reconstituted formulation according to claim 22, comprising 50-300 mg/ml 2-hydroxypropyl-β-cyclodextrin.
25. A stabilized pharmaceutical formulation consisting of:
i. voriconazole;
ii. a substituted β-cyclodextrin characterized by a molar substitution of the β-cyclodextrin by 2-hydroxypropyl groups of more than 0.8;
iii. optionally pH adjusting agents; and
iv. optionally pharmaceutically acceptable diluents or solvents.
26. The stabilized pharmaceutical formulation according to claim 25, having a pH in the range of 4-7.
27. A stabilized pharmaceutical formulation comprising voriconazole and a substituted β-cyclodextrin having a pH of 4-7 when dissolved in a suitable diluent.
28. The stabilized pharmaceutical formulation according to claim 27, wherein the formulation further comprises citrate, acetate, tartrate and/or succinate buffers.
29. The stabilized pharmaceutical formulation according to claim 27, wherein the formulation further comprises citric, acetic, tartaric and/or succinic acids.
30. A method for stabilizing a composition comprising voriconazole, wherein the method comprises the steps of:
a. providing an aqueous solution of 2-hydroxypropyl-β-cyclodextrin having a molar substitution of the β-cyclodextrin by hydroxypropyl groups of more than 0.8;
b. adding voriconazole;
c. optionally adjusting the pH; and
d. optionally lyophilizing the obtained stabilized composition.
31. The method according to claim 28, provided that the composition does not comprise lactose.
32. (canceled)
33. (canceled)
US14/760,260 2013-01-11 2014-01-09 Voriconazole inclusion complexes Abandoned US20150352112A1 (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11141417B2 (en) 2013-01-11 2021-10-12 Xellia Pharmaceuticals Aps Voriconazole inclusion complexes
CN115998675A (en) * 2022-12-26 2023-04-25 沈阳兴齐眼药股份有限公司 Voriconazole eye drops, preparation method and application thereof

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106117186B (en) * 2016-06-12 2018-08-24 重庆莱美隆宇药业有限公司 A kind of preparation method of voriconazole and its intermediate

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2018866A (en) * 1928-11-08 1935-10-29 Philip L O'donnell Folding machine and method of folding
WO2011020605A1 (en) * 2009-08-19 2011-02-24 Ratiopharm Gmbh Process for the production of coevaporates and complexes comprising voriconazole and cyclodextrin
WO2012171561A1 (en) * 2011-06-15 2012-12-20 Synthon Bv Stabilized voriconazole composition

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9713149D0 (en) 1997-06-21 1997-08-27 Pfizer Ltd Pharmaceutical formulations
CN1788725A (en) * 2004-12-15 2006-06-21 北京博尔达生物技术开发有限公司 Voriconazole freeze-drying powder injection and its preparation process
EP2018866A1 (en) 2007-07-27 2009-01-28 Sandoz AG Pharmaceutical compositions containing voriconazole
CN101849905A (en) * 2009-03-30 2010-10-06 河南省眼科研究所 Voriconazole eye drops and preparation method thereof
US20120065255A1 (en) * 2009-10-19 2012-03-15 Nagesh Palepu Cabazitaxel formulations and methods of preparing thereof
EP2470529B1 (en) * 2009-12-30 2014-11-19 Medichem, S.A. A 1-(1h-1,2,4-triazol-1-yl)butan-2-ol derivative for pharmaceutical use, and the use of a 1-(1h-1,2,4-triazol-1-yl)butan-2-ol derivate with substantially undefined crystal shape for preparing said 1-(1h-1,2,4-triazol-1-yl)butan-2-ol derivative
EP2409699B1 (en) * 2010-07-23 2014-04-30 Combino Pharm, S.L. Stable compositions of voriconazole
CN102058519B (en) * 2010-11-19 2013-01-02 苏州特瑞药业有限公司 Voriconazole slow-release suppository and preparation method thereof
US20150352112A1 (en) 2013-01-11 2015-12-10 Xellia Pharmaceuticals Aps Voriconazole inclusion complexes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2018866A (en) * 1928-11-08 1935-10-29 Philip L O'donnell Folding machine and method of folding
WO2011020605A1 (en) * 2009-08-19 2011-02-24 Ratiopharm Gmbh Process for the production of coevaporates and complexes comprising voriconazole and cyclodextrin
WO2012171561A1 (en) * 2011-06-15 2012-12-20 Synthon Bv Stabilized voriconazole composition

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11141417B2 (en) 2013-01-11 2021-10-12 Xellia Pharmaceuticals Aps Voriconazole inclusion complexes
CN115998675A (en) * 2022-12-26 2023-04-25 沈阳兴齐眼药股份有限公司 Voriconazole eye drops, preparation method and application thereof

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