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US20100275650A1 - Method and Apparatus for Recovery of Suspended Biological Material - Google Patents

Method and Apparatus for Recovery of Suspended Biological Material Download PDF

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Publication number
US20100275650A1
US20100275650A1 US12/774,439 US77443910A US2010275650A1 US 20100275650 A1 US20100275650 A1 US 20100275650A1 US 77443910 A US77443910 A US 77443910A US 2010275650 A1 US2010275650 A1 US 2010275650A1
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US
United States
Prior art keywords
suspension solution
biological material
container
filter
filter means
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US12/774,439
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English (en)
Inventor
Lawrence William Hirst
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2007906048A external-priority patent/AU2007906048A0/en
Application filed by Individual filed Critical Individual
Publication of US20100275650A1 publication Critical patent/US20100275650A1/en
Abandoned legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/71—Suction drainage systems
    • A61M1/79—Filters for solid matter
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2205/00—General characteristics of the apparatus
    • A61M2205/12—General characteristics of the apparatus with interchangeable cassettes forming partially or totally the fluid circuit
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2210/00—Anatomical parts of the body
    • A61M2210/06—Head
    • A61M2210/0612—Eyes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T29/00—Metal working
    • Y10T29/49—Method of mechanical manufacture
    • Y10T29/49588—Jewelry or locket making

Definitions

  • the present invention relates generally to a method of providing a promotional avenue for medical clinics by way of producing a personalized keepsake for their clients.
  • a keepsake of this kind may be considered a goodwill or promotional tool for a service offering.
  • a dental practitioner or other provider may actively promote the encapsulation or presentation of a first tooth as a special service, in order to attract business or simply differentiate the services of that outlet by developing goodwill with the client.
  • cataract surgery has become extremely common and is considered routine day surgery, with volumes of procedures and consequently numbers of clinical providers large and increasing.
  • a commercial pressure exists for clinics to secure more patients than their competitors, by way of clinical conditions or other offerings.
  • Cataract surgery is commonly conducted via a procedure call phacoemulsification, whereby the patient's clouded lens is broken up and removed via a tube through a small incision in the eye.
  • the lens material is broken into small fragments by ultrasonic pulses and is ejected via suction into a containment cassette located within the operating machine.
  • the cassettes accumulate the lens material and the fluid media used to irrigate the procedure, and the individual patient cassette is normally removed and discarded following the procedure.
  • the phacoemulsification method has become the standard procedure for routine cataract surgery, and the cassette-type collection of fluid and lens material is common, though the exact configuration of the equipment and performance varies. Importantly, the ability remains, by way of removing a connection or other port, to access the material accumulated within the cassette for most, if not all types of phacoemulsification systems.
  • the present invention is directed to a method and apparatus for recovery of suspended biological material, which may at least partially overcome at least one of the abovementioned disadvantages or provide the consumer with a useful or commercial choice.
  • the present invention in one form, resides broadly in an apparatus for recovery of suspended biological material from a solution, the apparatus including a suspension solution container to temporarily hold the suspension solution, extraction means to apply extractive force to the container to extract the suspension solution temporarily held therein and the filter means sized to separate the suspended biological material from the suspension solution.
  • the invention resides in a phacoemulsification separation apparatus for recovery of suspended biological material from a solution formed during phacoemulsification, the apparatus including a suspension solution container to temporarily hold the solution, extraction means to apply extractive force to the container to extract the solution temporarily held therein and the filter means sized to separate the suspended biological material from the solution.
  • the invention resides in a method for recovery of suspended biological material from a solution the method including the steps of a temporarily collecting the solution in a solution container, applying extractive force to the container to extract the solution temporarily held therein and filtering the solution through filter means sized to separate the suspended biological material from the solution.
  • the invention resides in a method for creation of a keepsake or memento from biological material recovered following removal during a medical procedure, the method including the steps of collecting the biological material in a solution, temporarily collecting the solution in a solution container, applying extractive force to the container to extract the solution temporarily held therein and filtering the solution through filter means sized to separate the suspended biological material as a filtrate from the solution, removing the filtrate from the filter means into a container, filling of the container with a measured amount of stabilizer fluid, and capping the container to seal the container.
  • the invention relates to an apparatus for recovery of a suspended biological material from a solution.
  • a phacoemulsification procedure results in a collection of the biological material in an irrigation fluid.
  • the fluid and biological material normally collected in a container, commonly known in the art as a cassette.
  • the cassette is normally quite small, approximately the size of B5 stationery and is normally manufactured of a transparent plastic.
  • the cassette has at least one opening in order to accept the collected material and irrigation fluid.
  • the cassette will preferably be removed to a controlled environment away from the patient, for the collection of the biological material.
  • the cassette will preferably be considered as a primary collection vessel.
  • the present invention includes extraction means.
  • the extraction means is normally provided as a plurality of components and connected to perform the function.
  • connection means will normally be provided to connect to the opening of the cassette through which the contents of the cassette can be removed. Normally, substantially all of the contents of the cassette are removed and the separation of the biological material from the remainder of the solution will be undertaken coincident with the removal from the cassette. Further, the biological material will normally be retained and the irrigation fluid will normally be wasted.
  • the connection means preferably includes a shaped connector.
  • the shaped connector will normally be provided in the form of a tapered stopper.
  • a particularly preferred form of the tapered stopper is a frustoconical member with a central bore extending through the stopper from a larger circular face to a smaller circular face.
  • the stopper will be manufactured from a resilient material in order to adapt the stopper to mate with openings of different dimension. The resilient material also typically creates an air/water proof seal between the stopper and the cassette opening.
  • the degree to which the stopper is tapered and the resilience of the material used may vary.
  • the stopper will provide a simple and quick connection which is air/fluid tight and typically will not require special training in order to connect the stopper to the cassette.
  • the extraction means also preferably includes a fluid conveyance tube.
  • the fluid conveyance tube is preferably attached to the stopper, normally by insertion of at least a portion of the tube into the bore of the stopper.
  • the fluid conveyance tube is also preferably associated with filter means.
  • the filter means is provided in an in-line configuration such that fluid extracted from the cassette must pass through the filter means.
  • a preferred form of filter means is provided in a two-part housing including an upper and lower housing portion with the upper and lower housing portion designated with reference to the flow direction and the lower housing portion being downstream of the upper housing portion.
  • each portion of the two-part housing is connectable to the other portion to allow separation.
  • a threaded connection is preferred but is not the only type of connection which may be used.
  • Grip means may be provided on one or both of the housing portions, preferably both.
  • Each portion of the filter housing will typically be manufactured from a plastic material, preferably a transparent plastic to enable a user to monitor the filtration process.
  • the filter housing will typically include a filter screen or similar.
  • the filter screen is preferably convex to the flow direction. Normally, the filter screen will be attached peripherally to the lower portion of the filter housing. It is particularly preferred that an upper surface of the filter screen be easily accessible with minimal exposed edges, corners or recesses in which material can be caught during removal which may limit the amount of collected biological material.
  • the openings in the filter screen will typically sized to separate the biological material particles large enough to be visible to the naked eye.
  • the phacoemulsification procedure results in material similar in size to fine sand and with a density which is suitably low to enable gentle agitation of the cassette during the removal process to produce a relatively homogenous mixture of the biological particles and irrigation fluid.
  • the filter screen is a stainless steel screen having openings of approximately 0.4 mm in diameter.
  • the filter will normally be connected in line with a fluid conveyance tube leading from the stopper to the upper portion and a second fluid conveyance tube leading from the lower portion of the filter for the waste irrigation fluid.
  • a waste outlet Located downstream of the filter is preferably a waste outlet and also a means to apply extractive force to the cassette and its contents.
  • the extractive force is normally applied through the use or application of a vacuum to the cassette although the cassette may alternatively be emptied by gravity draining for example or other similar process.
  • the preferred means for applying the vacuum is a syringe or similar, preferably of approximately 30 mL in capacity. Other means may be used alternatively such as a plumbed vacuum, Venturi or similar.
  • a common volume of the cassette used in the phacoemulsification procedure is between 50-200 mL and the irrigation liquid is normally a balanced salt solution.
  • an in-line check valve or non-return valve is included between the filter and the means for applying extractive force to enable multiple extractions to remove and expel waste irrigation fluid from the cassette.
  • the waste outlet is provided in the form of a waste tube connected downstream of the check valve or nonreturn valve but prior to the means for applying extractive force. Where a waste tube is provided, the free end of the waste tube may be weighted to ensure the directionality of the expelled stream.
  • the biological material will typically accumulate on the filter screen.
  • the filter housing can then be opened to expose the collected material on the screen.
  • the collected material can then be removed, normally scraped from the screen using an appropriate instrument.
  • An example of an appropriate instrument is a spatula, preferably shaped to enable manipulation of the collected material into a secondary collection vessel.
  • the preferred form of secondary collection vessel is typically jewellery or a wearable memento.
  • a particularly preferred form is that of a vial with a cap or the closure.
  • the vial is preferably a wearable vial such as a pendant, hearings, cufflinks or other. Whilst this embodiment relates to the collection of relatively small amounts of biological material, the method and apparatus of the present invention may be expanded to incorporate larger volumes of biological material into more substantial secondary collection vessels such as paper weights or other items.
  • a funnel or similar will normally be provided or used according to the method.
  • the funnel is normally located in all relative to an opening of the secondary collection vessel.
  • the cap or cover of the secondary collection vessel is preferably permanently attached to the vessel once the biological material (and any other material) has been inserted into the secondary collection vessel.
  • the means or method of fixing the cap to the vessel may be any suitable means including using an engineered tolerance closure or a suitable fast acting adhesive or bonding agent.
  • fluid will be added to the secondary collection vessel prior to sealing.
  • the fluid will stabilise the biological material and may also act to disperse the material within the vessel.
  • a preferred form of fluid is an approximately 70% alcohol solution which also has an added benefit of a low vaporisation temperature thereby reducing the amount of residue should any spillage or overflow occur.
  • the fluid may be added by any manner of application to the secondary collection vessel including from a bulk source or from a metered dose applicator such as a syringe, squirt bottle or the like.
  • the secondary collection vessel may be temporarily housed or arranged such that handling or correct orientation of the vessel is not required during the transfer of the material.
  • a handling fixture may be provided which may incorporate a broad base for stability and may be internally configured to align and retain a variety of forms of secondary collection vessel.
  • the secondary collection vessel will be retained in an upright position by the handling fixture by provision of a corresponding shaped opening in the handling fixture.
  • the handling fixture is preferably transparent is such that the secondary collection vessel (and other elements) are visible when located in the handling fixture.
  • a preferred configuration of includes a lower portion to at least temporarily hold the secondary collection vessel.
  • the lower portion also has a receiving opening and a shaped cavity internally configured to align and retain various forms of secondary collection vessel.
  • At least one upper portion is provided and also includes a shaped receiving opening.
  • the shaped opening in the at least one upper portion is internally configured to align and retain a cap for the secondary collection vessel.
  • there will be a pair of interchangeable upper portions a first upper portion used in conjunction with the lower portion in order to insert material and fluid into the secondary collection vessel and a second upper portion used to cap the secondary collection vessel once the first upper portion has been removed from the lower portion.
  • the upper and lower portions may have corresponding shaped means in order to positively align the upper and lower portions during use.
  • the most preferred process following the separation of the biological material includes the steps of:
  • the elements described above may be supplied to the clinic facility in kit form, such that re-usable and consumable components may be organized and waste minimized. It is anticipated that the extraction and filter components, plus the orientation fixtures suited to the various jewellery formats are re-usable items, requiring only a general flush and clean between applications or periodically, and the jewelry components, such as a variety or pendant bodies and caps may be supplied in bulk form, for selection according to patient requirements. Enclosure elements (jewelry pieces or otherwise) may be designed to accommodate a modular assembly system, allowing customization of the final presented product. Various accessories for the jewelry item may also be provided, such as necklaces, cufflink findings, clasps, pins or strings.
  • the product may be provided with a marking or otherwise applied graphic or labeling element which identifies the clinic or practitioner responsible for the procedure and supply.
  • This marking may be applied at component production level, such as embossing on plastic parts, or by a post-applied print or label affixed following assembly of the product.
  • the processing of the lens material, from reclamation from the phacoemulsification cassette to presentation to the patient should be a very rapid process, requiring minimal training and dexterity of the operator. Given that the patient is almost immediately mobile and able to leave the surgery within 15 minutes to half and hour of surgery, the process should preferably allow the keepsake to leave with the patient and should also not overly consume the time of clinic staff.
  • Alternate methods of lens or other material encapsulation may include incorporation into a rapid-curing resin or other means of capturing and sealing the material.
  • the capture of the material may be designed to maximize the visual impact of the keepsake, by addition of sparkling features or materials that impart flow, motion or other eye-catching or novel features.
  • a high-end application of the concept may include a longer or more involved method of material processing, such as resin impregnation or casting, such that the finished product is not provided immediately and the finished piece may be much more complex, functional or visually spectacular.
  • Provision of this system either as an immediate or follow-up patient service, must be considered within the context of a clinic marketing strategy and must consider the implications of privacy, patient consent and health and safety aspects of the use of human biological material.
  • FIG. 1 illustrates a preferred embodiment of an extraction kit according to the present invention.
  • FIG. 2 is a perspective illustration of a preferred filter used in the extraction kit illustrated in FIG. 1 opened to show the filter screen.
  • FIG. 3 is a perspective view of the filter screen as illustrated in FIG. 2 showing the biological material collected on the filter screen.
  • FIG. 4 illustrates a preferred step in the method of collection of the biological material namely using a spatula to remove the material from the filter screen.
  • FIG. 5 is a perspective view of two preferred embodiments of vials and respective caps which may be used in the present invention.
  • FIG. 6 is a perspective view of the vials and caps of FIG. 5 in a capped condition.
  • FIG. 7 is a perspective view from the front of a preferred embodiment of handling fixture used according to the present invention.
  • FIG. 8 is a perspective view of the handling fixture illustrated in FIG. 7 with a funnel in position.
  • FIG. 9 is a perspective view of the handling fixture illustrated in FIGS. 7 and 8 showing an upper portion of the handling fixture of the preferred embodiment used to close the vial.
  • FIG. 10 is an axonometric view of an alternative embodiment of an extraction kit of the present invention dispensing the collected material into a vial.
  • FIG. 11 is an axonometric view of an alternative embodiment of an extraction kit of the present invention.
  • FIG. 12 is an axonometric view of an alternative embodiment of the filter mechanism according to a preferred embodiment.
  • FIG. 13 is an axonometric view of the filter mechanism of FIG. 12 dispensing the collected material into a vial.
  • FIG. 14 is a sectional view of an alternative embodiment of the filter mechanism illustrated in FIG. 12 along line A-A.
  • FIG. 15 is a sectional view of the filter mechanism illustrated in FIG. 13 along line B-B dispensing the collected material into a vial.
  • a method and apparatus for creation of a keepsake or memento from biological material recovered following removal during a medical procedure, particularly phacoemulsification, is provided.
  • the phacoemulsification method has become the standard procedure for routine cataract surgery, and the collection of fluid and lens material removed during the procedure into a collection cassette 10 is common, though the exact configuration of the equipment and performance varies. Importantly, the ability remains, by way of removing a connection or other port, to access the material accumulated within the cassette 10 for most, if not all types of phacoemulsification systems.
  • this access includes the use of a tapered, resilient stopper 11 which provides a sealed connection to an extraction tube 12 via the appropriate port or opening in the cassette 10 .
  • the taper and resilience of the stopper 11 should accommodate various port sizes and or shapes, depending on the phacoemulsification system.
  • the extracted fluid is entrained via the tube 12 through a filtration component 13 by way of a vacuum or gravity draining.
  • suction to ensure rapid extraction is applied by a large syringe 14 .
  • a common volume of material is 50 to 200 mL of balanced salt solution, thus, the preferred embodiment includes vacuum extraction by way of a large syringe (30 mL) and a suitable check valve 15 enabling multiple pump strokes to alternately suck and expel waste irrigation fluid.
  • a waste tube 16 is provided with a weight 17 to ensure the waste stream is directed to an appropriate drain.
  • the preferred filter 13 is provided in two parts with an upper 18 and lower 19 portion (defined by their relative position in the flow path with the lower portion downstream), which are formed or otherwise labeled to indicate the flow direction therein.
  • the portions are easily separable by a user by means of a knurled or grippable edges, in order to access the collected lens material without loss or contamination.
  • the filter screen 20 located in the filter 13 is sufficiently coarse to only collect particles large enough to be easily visible. It is common for lens particles 21 delivered by phacoemulsification to be sized similarly to fine sand, with a density suitably low to enable the cassette 10 to be agitated gently to produce a relatively homogenous mixture of particles 21 and suspension or irrigation fluid.
  • the illustrated preferred embodiment incorporating a stainless steel filter screen 20 has apertures approximately 0.4 mm diameter, which captures a suitable amount of material for processing.
  • the lens particles/material 21 accumulates on the filter screen 20 within the filter 13 .
  • opening of the filter 13 exposes the filter screen 20 such that the lens particles/material 21 which tends to cling to the screen 20 face can be scraped away for collection as illustrated in FIG. 4 .
  • the filter screen 20 is exposed such that the entire face is accessible and no edges or corners limit the amount of collected material that may be scraped away.
  • a spatula 22 or other suitable tool may be provided to scrape the filter screen 20 .
  • This tool may also be shaped to enable manipulation of the lens material (which retains the consistency of damp brown sugar) into a secondary collection vessel 23 .
  • the secondary collection vessel 23 of the illustrated embodiment takes the form of a piece of jewellery or wearable memento.
  • the lens particles/material 21 are loaded into the secondary container 23 in a manner that is hygienic and safe for the operator.
  • the spatula 22 is configured to be received by a small funnel 24 located partially in the open neck of the secondary collection vessel 23 , such that no material is lost in the process.
  • FIGS. 5 and 6 illustrate preferred embodiments of the secondary collection vessel 23 in the form of jewellery pieces which as illustrated, take the form of a vial 25 with a cap 26 , which, when capped, can be worn or carried as a pendant.
  • the jewellery pieces, particularly the vials 25 can be housed or arranged such that handling or correct orientation of the small parts is not required.
  • FIG. 7 A preferred configuration of equipment to achieve this is illustrated in FIG. 7 in which the vial 25 is retained in an upright position by a handling fixture.
  • the handling fixture illustrated includes an upper 27 and a lower 28 portion, with the lower portion 28 incorporating a broad base 29 for stability.
  • the lower portion 28 also has a receiving opening 30 and shaped cavity (not shown) for a vial 25 , internally configured to align and retain various vial forms.
  • the upper portion 27 also includes a shaped receiving opening 31 .
  • the shaped opening 31 in the upper portion 27 is internally configured to align and retain a cap 26 for the vial 25 .
  • the handling fixture portions 27 , 28 of the illustrated embodiment are transparent, such that the vial and other elements are visible.
  • an amount of a fluid to both biologically stabilize and allow the lens particles/material 21 to disperse and drift in the vial 25 is added.
  • a suitable fluid for both these actions is 70% alcohol, which has the added benefit of not leaving a residue should any spillage or overflow occur due to its rapid vaporisation.
  • the handling fixture may also allow orientation of the cap 26 , such that the filling and correct closure process requires no contact between reclaimed lens particles/material 21 and the clinic operator.
  • Fixation of the cap 26 to the vial 25 may be by an engineered tolerance closure, or a suitable fast-acting adhesive or bonding agent.
  • FIGS. 10 to 15 An alternative embodiment of the device for recovery of the lens particles/material from the cassette is illustrated in FIGS. 10 to 15 .
  • the syringe 14 is fitted to the thumb end of a filter/plunger assembly 33 .
  • the tube 12 with bag spike 34 is fitted to the opposing inlet end of the filter/plunger assembly 33 .
  • the bag spike 34 is configured with a removable rubberised stopper 11 , enabling the bag spike 34 to be configured to access a soft bag (utilising the spike 36 ), a cassette (utilising the stopper 11 ), or tubing.
  • the fluid is drawn through the filter/plunger assembly 33 by the syringe 14 , such that particulate matter is collected by the filter element 13 within the inlet end 36 of the filter/plunger assembly 33 .
  • the filter element is typically cellulose acetate fibre.
  • the filter element 13 may be set a little back from the filter nose opening 37 , such that collected material is entrapped by the plastic enclosure of the nose opening 37 , rather than relying solely on the stickiness of the agglomeration in the filter.
  • the syringe 14 is removed from the top of the plunger/filter assembly 33 , and the tube 12 carefully removed from the inlet end 36 .
  • the syringe 14 may be reconnected directly to the tubing to expel the (filtered) fluid.
  • the plunger/filter assembly 33 may then be manipulated like a syringe with the wings 38 providing a bearing surface for the fingers, and the thumb providing a force on the central plunger 39 .
  • the nose of the plunger/filter assembly 33 is aligned with or projected into a vial 25 or receptacle as illustrated in FIG. 10 , 13 or 15 , typically some form of decorative/jewellery/keepsake item, in which there is a suitably sized opening and an internal void which may be sealed with some form of cap.
  • a commonly available glass vial and cap system is used.
  • the nose of the plunger/filter assembly 33 is inserted to the vial 25 and the plunger 39 pressed hard, which forces the plunger stem 40 forward, first compressing the filter element 13 , then bursting the nose of the plunger/filter assembly 33 , such that the filter element 13 and collected material is expelled through the hole into the vial 25 .
  • the plunger/filter assembly 33 is then discarded.
  • the vial 25 is filled with a liquid of which pure acetone is a preferred form, such that the filter element 13 is covered and no bubbles remain.
  • the vial 25 is capped, preferably to eliminate air bubbles, and permanently sealed. A decorative cap or jewellery finding may be added to the vial 25 .
  • the preferred acetone is a solvent to the preferred cellulose acetate filter element, which dissolves, leaving the recovered cataract lens material floating freely in the vial 25 .
  • the preferred process therefore includes the steps of:

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US12/774,439 2007-11-05 2010-05-05 Method and Apparatus for Recovery of Suspended Biological Material Abandoned US20100275650A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2007906048 2007-11-05
AU2007906048A AU2007906048A0 (en) 2007-11-05 A Method And Apparatus For Recovery Of Suspended Biological Material
PCT/AU2008/001643 WO2009059363A1 (fr) 2007-11-05 2008-11-05 Procédé et appareil pour la récupération de matière biologique en suspension

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Application Number Title Priority Date Filing Date
PCT/AU2008/001643 Continuation-In-Part WO2009059363A1 (fr) 2007-11-05 2008-11-05 Procédé et appareil pour la récupération de matière biologique en suspension

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CA (1) CA2704846A1 (fr)
NZ (1) NZ585963A (fr)
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US20140290756A1 (en) * 2011-11-21 2014-10-02 Kautex Textron Gmbh & Co. Kg Secondary liquid container for a motor vehicle
CN113539007A (zh) * 2021-09-07 2021-10-22 中国人民解放军陆军特色医学中心 一种白内障教学用超声乳化白内障吸除训练器

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