UA148949U - METHOD OF ROOTING OF RIBES RUBRUM L. IN VITRO - Google Patents

Abstract

The method of rooting explants of red currants (Ribes rubrumL.) In vitro includes the preparation of a nutrient medium containing microsalts, sucrose and mesoinositol, and aminoacetic acid, vitamins (B1, B6, PP), in addition, add naphthylacetic acid, zeatin, N-oxide 2,6-dimethylpyridine, humic acids and autoclaving, cooling and planting explants on the second or third day.

Description

The useful model belongs to biotechnological methods, namely the method of cell selection and, in particular, the microclonal propagation of ip myo plants, which provides ample opportunities for preserving the gene pool of plants, for creating the initial selection material of red currants (Nire5 gibgit I.).

There is a well-known method of growing ip migo plants, in which a growth regulator of an auxin nature is introduced into the nutrient medium for rooting in order to increase the rooting coefficient of cultivated ip migo plants, and as the latter, indolyl butyric acid is most often used (aut.sv. USSR M 1792270, class A 01 H 4/00, 29/04/91 N.M. Pyven, Melnychuk

H.G., Felaliev A.S. The method of rooting the shoots of nuts obtained from mito. Bul. M 4 dated 30.01.93. However, with this method, at the initial stage of rooting, there is often an increase in undifferentiated growth of tissues, inhibition of the processes of root formation and growth, which leads to a deterioration of rooting and an extension of the rooting period of plants.

Therefore, to improve rooting, various methods are used, for example, microshoots are soaked in a solution of a growth regulator (indolyl-butyric acid) on the surface of the agar medium (patent of the Russian Federation M2060646b cl. A 01 H 4/00, 1994 Turovskaya N.I., Pronina I. N.,

Matushkina O.V. The method of rooting the shoots of fruit crops obtained by ip mygo. Bul. M 15 dated 27.05.96). At the same time, this method is quite time-consuming, requires additional time spent on preparing the environment and creates a danger of contamination of the environment with harmful microflora.

The closest analogue is the method of rooting microshoots under conditions of ip migo, where oxybenzoic acid at a concentration of 14107 M was additionally introduced into the nutrient medium. The method of growing ip migo plants M.T. Upadyishev, statement 24.12.1999, publ. 10.12.2000, Bull. Mo. 34.- 12 Si.

The disadvantage of this method is the low yield of rooted regenerative plants.

The basis of a useful model is the task of creating a method of rooting explants of red currants (Nire5 gibgit I.) in the culture of ip mik, which includes the preparation of a nutrient medium.

The set task is solved by the fact that the method of rooting explants of red currants (Nire5 pgibgit I.) in the culture of ip migo includes the preparation of a nutrient medium that contains

From microsalts, sucrose and meso-inosit (increased concentrations), and aminoacetic acid (decreased ratio), vitamins (B1, Bb, PP). Additionally, it contains naphthylacetic acid, zeatin, M-oxide-2,6-dimethylpyridine and humic acids.

The nutrient medium is poured into test tubes and autoclaved at a pressure of 1.1 atm (temperature 121 "С) for 15-20 minutes. After the nutrient medium has cooled, usually on the second or third day, red currant shoots are planted on it (Virez5 gybgit

Gu.

Example. For rooting, a basic nutrient medium was used according to the prescription

Murashige-Skuga with reduced concentrations of mineral and organic components. In the specified environment, the concentration of vitamin VI increases! to 1.0 mg/l and add naphthylacetic acid at a concentration of 0.25 mg/l, zeatin at 0.5 mg/l, M-oxide of 2,6-dimethylpyridine at 0.001 mg/l and humic acids at a concentration of 18 mg/l. , the carbon source (sucrose) is reduced to 21,000 mg/l. The volume of the solution is brought up to 1 l, the pH is set to 5.6-5.8 and agar-agar (7 g/l) is added when heated. The nutrient medium is poured into test tubes and autoclaved at a pressure of 1.1 atm (temperature 121 7C) for 15-20 minutes. After cooling the nutrient medium, usually on the second or third day, red currant shoots are planted on it.

The use of the proposed nutrient medium makes it possible to obtain a new effect - to increase the output of rooted shoots, to improve the development of the root system of plants.

When preparing the nutrient medium, the basic medium was modified according to the Murashige-Skoog recipe, using data from the prototype medium. The characteristics of the media used are given in Table 1.

Table 1

Prescriptions of nutrient media 11111111 basic | prototype | modified potassium phosphoric acid, monosubstituted zinc sulfuric acid (205Oah 7HgO). | 86 | ..yu43 2 sh | 86 sodium molybdenum acid (Ma?MoO4 x ethylenediaminetetraacetate sodium ascorbic acid (С) 71711111 - 1111105 | 7-2 Ж ш BmСЗ«

Indolylbutyric acid FIMUI HER 10 HER 7-2 2ХЖХХ | -

P-Oxybenzoic acid.d 77771111 -0 1 1О6Ми1- (Naphthyloacetic acid(1-NOK).І ІІ 777717 - І. ЮБІБ.Б- | 025

Zeatin//////711111111111111Ї111171-0 111111 - 1 05 2 (« oM-oxide-2,b-dimethylpyridine.dH | - 01111711 - 1... 00012 shshC-

Humic acid ///7777777111111Ї1111-0 1111111 - 1. Yu Yu.180 2

The proposed medium differs significantly from the prototype in the increased amount of microsalts and meso-inositol, in addition, it is supplemented with a different ratio of aminoacetic acid, vitamins (VI, Vb, PP), supplemented with auxin 1-NOK, zeatin, M-oxide-2,6-dimethylpyridine and humic acids

The use of a modified nutrient medium, unlike the prototype, shortens the start of rhizogenesis by two days and increases the number of rooted plants from 69.3 956 (prototype) to 92.2 95 (modified) (Table 2).

Table 2

Rhizogenesis of red currant plants (Virez hybgit I.) in the culture of ip migo

Explants that 2, and Average number

Learned numbers, the beginning was formed by

Environment. rooted pcs. rhizogenesis, root system, etc.; explants, pcs. 50.1-6.5 876541 69.323.6 124.753.4 92.2x2.3 178.022.5 70.54 13 0.153.3

Explants that did not form a root system were culled, thereby artificially selecting genotypes capable of rooting ip myo.

Claims (1)

USEFUL MODEL FORMULA A method of rooting explants of red currants (Virez5 girgiti. .) ip mio, which differs in that it includes the preparation of a nutrient medium containing microsalts, sucrose and meso-inosit, and aminoacetic acid, vitamins (B1, Vb, PP), in addition add naphthylacetic acid, zeatin, M-oxide-2,6-dimethylpyridine, humic acids and carry out autoclaving, cooling and planting of explants on the second or third day.