TWM393167U - Multi-coating macro-granule structure comprising probiotics - Google Patents

Abstract

The invention discloses a multi-coating macro-granule structure comprising probiotics. The structure according to the present invention contains a core, a probiotic layer, a nutritional and functional layer, and a protective layer arranged form inside to outside in order. Besides, the probiotic layer is coated with the core, and the nutritional and functional layer is coated with the probiotic layer which coated with the core. Furthermore, the protective layer is coated with the nutritional and functional layer which coated with the probiotic layer.

Description

M393167 V. New description: [New technical field] This creation is about a multi-layered particle structure, a multi-layered particle structure with probiotics. Do not know about [previous technology] At present, the common probiotic products on the market, the roots and sputum process, are divided into the following four types: powder;; ^ technology embedded type probiotics, crystal ball probiotics Double-layer micro-embedded type: soil mites, giant end probiotics refers to probiotics that have not been embedded, 1=^. Powder ::: Durable, its bacteria are easily destroyed. The giant-embedded probiotics: hard-encapsulated probiotics can protect the bacteria from the bacteria. The timing of releasing the bacteria is not good. Package: The material is then encapsulated with probiotics in microcapsules. Dissolved, and its price is high-layer micro-embedded probiotics, which refers to the surface of probiotics coated with one; ^ shell, to isolate the bacteria from the outside air, reduce contact, and preserve the activity of the body, followed by Material coating - layer special _, burying bacteria can be resistant to stomach acid, total, six stations. _ θ has been established because the package has not yet established this package in the domestic probiotics related capsules still have to rely on the powder particles or - Agents lead to the domestic production of probiotics-related products, "the lack of Γ eight female 疋, resulting in short storage period, reduced viable counts and other shortcomings. Therefore, the stability of probiotic cells is not good, the storage period is short, the number of viable bacteria is reduced, the resistance of M393167 is strengthened, and the solvency in the Shengdao is improved. The new content is solved θ in view of the above-mentioned problems of the prior art, The purpose of the creation is to provide a structure with probiotics and red soil layers, to maintain the stability of probiotics: the problem of short foot, short storage period, and reduced viable count, and to strengthen the ability of bacteria to resist acid and intestinal transit. The bacteria are more awkward and can enhance the competitiveness of our related industries in the world. According to the purpose of the present invention, a multi-layered microparticle structure having probiotics is provided, the structure comprising a core, a probiotic layer, a nutrient function layer and a protective layer in sequence from the inside to the outside. Wherein the probiotic layer; coated on the outer core' and the nutrient function layer is coated outside the probiotic layer, and the protective layer is re-coated outside the nutrient function layer. According to the above, the multi-layered microparticle structure with probiotics can have one or more of the following advantages: (1) The multi-layered microparticle structure with probiotics contains a protective layer, so that it can be solved in the stomach acid environment. The problem of destruction is explained by the more soluble in the intestinal environment. (2) The multi-layered micro-structure of the probiotics contains a nutrient function layer. Therefore, not only can the probiotics nutrient f be provided, but also the human body can be healthy and youthful after being ingested. 5 M393167 (3) The multi-layered micro-particle structure of this probiotic has a probiotic layer, so the whole body quality can be adjusted, the function of the digestive tract can be maintained and the intestinal flora can be changed. (4) The multi-layered particle structure of the probiotic can increase the stability of the secondary processing of the cell and its storage stability. [Embodiment] Hereinafter, embodiments of the multi-layered particle structure having probiotics according to the present invention will be described with reference to the related drawings. For the sake of easy understanding, the same elements in the following embodiments are denoted by the same reference numerals. Please refer to Figure 1 for a schematic diagram of the composition of the multi-Zhan particle structure with probiotics. In the figure, the multi-layered microparticle structure of the probiotic comprises the core 1, the probiotic layer 2, the nutrient function layer 3 and the protective layer 4 from the inside to the outside, wherein the probiotic layer 2 is coated on the core 1 and is nutritious. The functional layer 3 is coated on the surface of the probiotic layer 2, and the protective layer 4 is further coated on the outside of the nutrient function layer 3. Among them, the core 1 is a carbohydrate containing sucrose, glucose or fructose, and the probiotic layer 2 comprises LGG (L. rhamnosus GG) lactic acid bacteria, Lactobacillus paracasei, Bifidobacterium longum or Bifidobacterium bifidum, and the nutrient function layer 3 contains collagen, Ganoderma lucidum extract, fructooligosaccharide, inulin, Dali extract or carboxymethyl cellulose, chlorella, Hyaluronic acid, glucosamine, superoxide dismutase (SOD) or brown bath polysaccharide, in addition, the protective layer is an enteric film material containing shellac, hydroxypropyl methylcellulose Phthalate, HPMCP), corn M393167 protein (zein), gelatin, acacia, collagen or sodium alginate. Example 2: The preferred embodiment of the present invention is a micro-bead of the core-embedded microparticles. The material of the microparticles embedded in the core of the sugar core comprises a sugar core, (10) GG) lactic acid bacteria 'collagen and extracts of ganoderma lucidum and enteric properties. Membrane material. Solubility assessment test: • Take 1 〇 S sample into 90 mL Mcilvaine buffer (pH 6 8

Mcilvaine's Buffer), the magnet was slowly stirred at 80 rpm until it completely disintegrated and the time required for disintegration was recorded. The results are shown in the table, in which zein is an enteric film material Particle In granule III) And 10% shellac is the enteric film material% (Macro-granule VI) and microparticle VII (Macro-granule VII) 'when it disintegrates, the door is 60 minutes, and 10% shellac is the intestine. The particles of the soluble film material (Macro-granule IV) and the particles γ φ (Macro-granule V) with 2% shellac as the enteric film material have a disintegration time of 30 minutes, and the above disintegration time is good. - Appropriate timing of bacterial release in the intestine. This result also shows that zein and gelatin are suitable as enteric film materials. ~ 7 M393167 Table 1: Embedding microparticles disintegration (solubility) Test sample Disintegration time Particles III Within 60 minutes Microparticles IV Within 30 minutes Particles V Within 30 minutes Particles VI Within 60 minutes Particles VII Within 1 minute of original lactic acid bacteria powder 1 minute Internal acid resistance evaluation test: The acid resistance evaluation test is further divided into no acid treatment and acid treatment test. Without acid treatment, 1 g of sample was added to 100 mL of Mcilvaine's buffer (pH 6.8 Mcilvaine's Buffer), and the magnet was slowly stirred at 80 rpm until completely disintegrated. After appropriate dilution, the surviving bacteria were counted by pouring plate method. number. In addition, the acid treatment test refers to taking 1 g of sample in 100 mL of phosphate buffered saline (PBS, pH 1.5), shaking at 50 rpm for 1 hour, and centrifuging at 12,000 rpm for 15 minutes. Then, the upper simulated gastric juice is removed, and the lower layer of the precipitate is added to 100 mL of simulated intestinal juice and suspended, followed by shaking at 80 rpm. After appropriate dilution, the number of surviving bacteria is counted by a pour plate method and the survival rate is calculated. The survival rate algorithm is the number of bacteria without acid treatment / the number of bacteria treated by acid x lOO%. The experimental results are shown in Table 2. Among them, the survival rate of M393167 GM VI and GM VII with sugar core as the core and 10% shellac as the enteric film is higher (85.17% and 80.24%), and 10% insects are also known. The glue is suitable for acid-resistant enteric film material. Table 2 Embedding microparticles acid-resistant test item Samples without acid treatment Number of cells treated with acid-treated cell survival rate (%) Particles III 1.24Χ10, υ 5.32X10, 0.43 Particles IV 1.13X10" 3.96Χ108 0.35 Particles V 2.25Χ10ιυ 8.39 Χ108 3.73 Particle VI 1.73X10y 1.51X10y 85.17 Particle VII 5.77Χ10ιυ 4.63ΧΚΓ 80.24 Original lactic acid bacteria powder 2.21Χ1011 6.65X10" 0.003 Moisture content analysis: Take 3g sample in sample dish, open the lid and place it in 105°C φ oven After drying for 4 hours, the water of the microparticles is evaporated. The lid is placed in an oven and the petri dish is taken out. The culture dish is placed in a closed container and a desiccant is added to the container. After the temperature of the particles is balanced with the room temperature, the scale is taken out. Heavy, and then placed in an oven at 105 ° C for 1 hour, placed in a closed container and then warmed up, take out the weight, repeat the above steps until the weight is no longer changed, and calculate the moisture content according to the following formula, the results show that The water content of all the particles is below 8%, which also shows that the product is not resistant to storage. 9 M393167 Moisture content = heavy before drying, dry weight X 100% Heavy before drying Water activity analysis: Take 2g of microparticles in the sample box, and the results show that the water activity is below 0.7, the problem of bacterial spoilage. As analyzed by the water activity analyzer, '' also means that the bacteria or mold can be improved. The above is merely illustrative and not limiting. Any changes or modifications that are made without departing from the spirit and scope of this creation shall be included in the scope of the appended patent application. [Simple description of the diagram] Figure 1 is a schematic diagram of the composition of the multi-layered microparticles with probiotics. [Main component symbol description] 1 : Core; 2 : M yJ. ±i Probiotic layer; Nutritional function layer; and Protective layer. 4

Claims (1)

Patent application scope: 1. A multi-layered particle structure with probiotics, comprising: a core; a layer of probiotics coated with the probiotic layer outside the core; a nutrient function layer 'the nutrient function layer package Covering the probiotic layer; and a protective layer 'the protective layer is coated outside the nutrient function layer. 2. A multi-layered microparticle structure having probiotics as described in claim 1 wherein the core is a carbohydrate. 3. The multi-layered microparticle structure having probiotics as described in claim 2, wherein the carbohydrate comprises sucrose, glucose or fructose. 4. The multi-layered microparticle structure having probiotics as described in claim 1, wherein the probiotic layer comprises LGG (Z. GG) lactic acid bacteria, dried lactic acid bacteria, and longum) Debirometer {Bifidobacterium bifidum). 5. The multi-layered microparticle structure having probiotics according to claim 1, wherein the nutrient function layer comprises collagen, ganoderma lucidum extract, fructooligosaccharide, inulin, soybean extract or carboxymethylcellulose (carboxymethyl cellulose), green algae polysaccharide, hyaluronic acid, glucosamine, superoxide dismutase (SOD) or brown bath polysaccharide. M393167. The multi-layered microparticle structure having a probiotic according to claim 1, wherein the protective layer is an enteric film material. 7. The multi-layered microparticle structure having a probiotic according to claim 6, wherein the enteric film material comprises shellac, hydroxypropyl methylcellulose phthalate (HPMCP), Zein, gelatin, gum arabic, collagen or sodium alginate. 12