TWI877068B - Cultivation method of low-protein rice - Google Patents
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本發明是關於一種稻米的栽培方法,特別是關於一種低蛋白稻米的栽培方法。The present invention relates to a method for cultivating rice, in particular to a method for cultivating low-protein rice.
對於慢性腎臟病患者而言,其腎臟無法有效地將蛋白質代謝後所產生的含氮廢物排出,致使含氮廢物可能堆積在血液中而對身體產生毒害,是以適量限制蛋白質的攝取量可以延緩腎功能的惡化。For patients with chronic kidney disease, their kidneys cannot effectively excrete nitrogenous waste products produced after protein metabolism, causing nitrogenous waste products to accumulate in the blood and become toxic to the body. Therefore, appropriately limiting protein intake can slow down the deterioration of kidney function.
為了降低含氮廢物對身體造成的傷害,醫師等專業人員多建議於慢性腎臟病患者的餐食中限制蛋白質的攝取量,並改以其他種類的食物來補充不足的熱量。由於米食為東方人的主食,但是米食中蛋白質的生物可用率不高,是以當蛋白質攝取量受到限制,慢性腎臟病患者應以優質的蛋白質作為主要首選,如雞肉、魚肉及黃豆製品等,以提高蛋白質吸收量。另可選用低蛋白澱粉(米粉、冬粉、粄條等)取代部分主食,既可維持熱量均衡也能避免蛋白質攝取過量。因此,從米食中攝取過多的蛋白質,反而不利於慢性腎臟病患者的飲食控制。In order to reduce the harm caused by nitrogenous waste to the body, doctors and other professionals often recommend limiting the protein intake in the meals of patients with chronic kidney disease and replacing them with other types of food to supplement the insufficient calories. Since rice is the staple food of Oriental people, but the bioavailability of protein in rice is not high, when protein intake is limited, patients with chronic kidney disease should choose high-quality protein as the main choice, such as chicken, fish and soy products, to increase protein absorption. Alternatively, low-protein starch (rice noodles, vermicelli, rice noodles, etc.) can be used to replace part of the staple food, which can maintain calorie balance and avoid excessive protein intake. Therefore, consuming too much protein from rice is not conducive to the dietary control of patients with chronic kidney disease.
為了解決上述問題,遂有業者將現行市面上的稻米以乾燥、蒸煮等後加工方式進行處理,以分解其中的蛋白質,或以添加微生物的方式對稻米進行發酵,以獲得具有較低蛋白質含量的米食。然而,以乾燥、蒸煮等方式對稻米進行處理不僅會破壞稻米中的其他營養成分,其加工方式亦較為繁雜,且加熱處理的過程中亦有可能使其他雜菌滋生,在營養層面及衛生考量等方面皆不盡理想。再者,以微生物發酵來降低稻米中的蛋白質的方式同樣可能改變其他營養成分的組成比例,且微生物本身或其二次代謝物亦有引起腸胃不適、過敏等反應的疑慮,致使以上述方式進行後加工而降低稻米中蛋白質含量的方法在產業應用上多有限制。In order to solve the above problems, some businesses have processed the rice currently on the market by drying, steaming, etc. to decompose the protein in it, or fermented the rice by adding microorganisms to obtain rice with a lower protein content. However, processing rice by drying, steaming, etc. will not only destroy other nutrients in the rice, but the processing method is also more complicated, and the heat treatment process may also cause other bacteria to grow, which is not ideal in terms of nutrition and hygiene considerations. Furthermore, the method of reducing the protein content in rice by microbial fermentation may also change the composition ratio of other nutrients, and there is also concern that the microorganisms themselves or their secondary metabolites may cause gastrointestinal discomfort, allergies and other reactions, resulting in many limitations in the industrial application of the method of reducing the protein content in rice by post-processing in the above manner.
因此,發展一種可栽培具有低蛋白含量的稻米,以供市場所需,實為一具有經濟價值之技術課題。Therefore, developing a rice with low protein content that can be cultivated to meet market demand is a technical issue with economic value.
本發明一實施方式提供一種低蛋白稻米的栽培方法,包含下述步驟。進行一整地步驟,其係於一耕地施用一第一肥料組合物,其中第一肥料組合物包含磷酸、氮與鉀,且基於磷酸為1重量百分比,氮為2.5~4.5重量百分比,鉀為1.5~3重量百分比。進行一插秧步驟,其係將一水稻幼株種植於耕地中。進行一第一栽培步驟,其係將水稻幼株於耕地中栽培15~30天並施用一第二肥料組合物,以得一水稻成株,其中第二肥料組合物於第一栽培步驟中的施用次數為至少一次,第二肥料組合物包含鎂、錳、鐵、硼、鋅與銅,且基於銅為1重量百分比,鎂為6~18重量百分比,錳為2~6重量百分比,鐵為1.5~6重量百分比,硼為1~3重量百分比,鋅為2~5重量百分比。進行一第二栽培步驟,其係將水稻成株於耕地中栽培15~30天並施用一第三肥料組合物,以使水稻成株進入繁殖期,且第三肥料組合物於第二栽培步驟中的施用次數為至少一次,其中:第三肥料組合物包含鎂、錳、鐵、硼、鋅、銅、磷酸、氮與鉀,且基於銅為1重量百分比,鎂為6~18重量百分比,錳為2~6重量百分比,鐵為1.5~6重量百分比,硼為1~3重量百分比,鋅為2~5重量百分比;及磷酸於第三肥料組合物中的重量百分比為磷酸於第一肥料組合物中的重量百分比的1/20~1/50,氮於第三肥料組合物中的重量百分比為氮於第一肥料組合物中的重量百分比的1/20~1/50,且鉀於第三肥料組合物中的重量百分比為鉀於第一肥料組合物中的重量百分比的1/20~1/50。進行一收穫步驟,其係收穫水稻成株,以得複數個低蛋白稻米,其中所述之低蛋白稻米的蛋白質含量為每100公克的低蛋白稻米中包含小於6公克的蛋白質。An embodiment of the present invention provides a method for cultivating low-protein rice, comprising the following steps: performing a land preparation step, which is to apply a first fertilizer composition to a cultivated land, wherein the first fertilizer composition comprises phosphoric acid, nitrogen and potassium, and the phosphoric acid is 1 weight percent, the nitrogen is 2.5-4.5 weight percent, and the potassium is 1.5-3 weight percent. Performing a rice transplanting step, which is to plant a rice seedling in the cultivated land. A first cultivation step is performed, wherein the rice seedlings are cultivated in a cultivated land for 15 to 30 days and a second fertilizer composition is applied to obtain a mature rice plant, wherein the second fertilizer composition is applied at least once in the first cultivation step, and the second fertilizer composition comprises magnesium, manganese, iron, boron, zinc and copper, and based on 1 weight percent of copper, 6 to 18 weight percent of magnesium, 2 to 6 weight percent of manganese, 1.5 to 6 weight percent of iron, 1 to 3 weight percent of boron and 2 to 5 weight percent of zinc. A second cultivation step is performed, wherein the mature rice plants are cultivated in cultivated land for 15 to 30 days and a third fertilizer composition is applied to the mature rice plants so that the mature rice plants enter a reproductive period, and the third fertilizer composition is applied at least once in the second cultivation step, wherein the third fertilizer composition comprises magnesium, manganese, iron, boron, zinc, copper, phosphoric acid, nitrogen and potassium, and based on 1 weight percent of copper, 6 to 18 weight percent of magnesium, 2 to 6 weight percent of manganese, and 1.5 to 6 weight percent of iron. , boron is 1-3 weight percent, zinc is 2-5 weight percent; and the weight percent of phosphoric acid in the third fertilizer composition is 1/20-1/50 of the weight percent of phosphoric acid in the first fertilizer composition, the weight percent of nitrogen in the third fertilizer composition is 1/20-1/50 of the weight percent of nitrogen in the first fertilizer composition, and the weight percent of potassium in the third fertilizer composition is 1/20-1/50 of the weight percent of potassium in the first fertilizer composition. A harvesting step is performed, which is to harvest the rice plants to obtain a plurality of low-protein rices, wherein the protein content of the low-protein rice is less than 6 grams of protein per 100 grams of low-protein rice.
依據前述之低蛋白稻米的栽培方法,其中耕地的一有機質含量可為0.5%~3.5%。According to the aforementioned method of cultivating low-protein rice, the organic matter content of the cultivated land can be 0.5%~3.5%.
依據前述之低蛋白稻米的栽培方法,其中耕地的一pH值可為5.5~7.5。According to the aforementioned method for cultivating low-protein rice, a pH value of the cultivated land may be 5.5-7.5.
依據前述之低蛋白稻米的栽培方法,其中水稻幼株可為一第一期作水稻或一第二期作水稻。According to the aforementioned method for cultivating low-protein rice, the rice plant can be a first-stage rice plant or a second-stage rice plant.
依據前述之低蛋白稻米的栽培方法,其中當水稻幼株為第一期作水稻時,第一栽培步驟可將水稻幼株於耕地中栽培20~30天。According to the aforementioned method for cultivating low-protein rice, when the rice plants are first-stage rice plants, the first cultivation step may be to cultivate the rice plants in cultivated land for 20 to 30 days.
依據前述之低蛋白稻米的栽培方法,其中當水稻幼株為第一期作水稻時,第二栽培步驟可將水稻成株於耕地中栽培15~25天。According to the aforementioned method for cultivating low-protein rice, when the rice seedlings are the first-stage rice plants, the second cultivation step may be to cultivate the mature rice plants in the cultivated land for 15 to 25 days.
依據前述之低蛋白稻米的栽培方法,其中當水稻幼株為第二期作水稻時,第一栽培步驟可將水稻幼株於耕地中栽培15~25天。According to the aforementioned method for cultivating low-protein rice, when the rice plants are second-stage rice plants, the first cultivation step may be to cultivate the rice plants in cultivated land for 15 to 25 days.
依據前述之低蛋白稻米的栽培方法,其中當水稻幼株為第二期作水稻時,第二栽培步驟係將水稻成株於耕地中栽培15~25天。According to the aforementioned method for cultivating low-protein rice, when the rice seedlings are second-stage rice, the second cultivation step is to cultivate the rice mature plants in cultivated land for 15 to 25 days.
依據前述之低蛋白稻米的栽培方法,其中第二肥料組合物可以葉面施肥的方式施用於水稻幼株。According to the aforementioned method for cultivating low-protein rice, the second fertilizer composition can be applied to young rice plants in the form of foliar fertilization.
依據前述之低蛋白稻米的栽培方法,其中第三肥料組合物可以葉面施肥的方式施用於水稻成株。According to the aforementioned method for cultivating low-protein rice, the third fertilizer composition can be applied to mature rice plants in the form of foliar fertilization.
藉此,本發明之低蛋白稻米的栽培方法在插秧步驟前先於耕地施用具有特定磷酸、氮與鉀之配比的第一肥料組合物,並於水稻幼株種植於耕地後依序提供第二肥料組合物與第三肥料組合物的三階段營養差異栽培方式,不僅可使水稻幼株健康生長,且栽培而得的低蛋白稻米具有較低的蛋白質含量,並可應用於慢性腎臟病患者的飲食控制計畫中,使其具有優異的市場應用潛力。Thus, the cultivation method of low-protein rice of the present invention applies a first fertilizer composition having a specific ratio of phosphate, nitrogen and potassium to the cultivated land before the rice seedling transplanting step, and sequentially provides a second fertilizer composition and a third fertilizer composition after the rice seedlings are planted in the cultivated land. The three-stage nutritional difference cultivation method not only enables the rice seedlings to grow healthily, but also the cultivated low-protein rice has a lower protein content and can be applied to the dietary control plan of patients with chronic kidney disease, making it have excellent market application potential.
以下將參照圖式示範說明本發明之具體試驗例,以利於本發明所屬領域之通常知識者,可在不需過度解讀與實驗的情形下完整利用並實踐本發明。然而,閱讀者應瞭解到,這些實務上的細節不應用以限制本發明,也就是說,在本發明部分試驗例中,這些實務上的細節是非必要的,而是用以說明如何實施本發明之材料與方法。The following will illustrate specific experimental examples of the present invention with reference to the drawings, so that those with ordinary knowledge in the field to which the present invention belongs can fully utilize and practice the present invention without excessive interpretation and experimentation. However, the reader should understand that these practical details should not be used to limit the present invention, that is, in some experimental examples of the present invention, these practical details are not necessary, but are used to illustrate how to implement the materials and methods of the present invention.
[本發明之低蛋白稻米的栽培方法][The cultivation method of low-protein rice of the present invention]
請參照第1圖,其係繪示本發明之低蛋白稻米的栽培方法100的步驟流程圖。低蛋白稻米的栽培方法100包含步驟110、步驟120、步驟130、步驟140以及步驟150。Please refer to FIG. 1 , which is a flow chart showing the steps of the
步驟110為進行一整地步驟,其係於一耕地施用一第一肥料組合物。詳細而言,在稻米進行栽種之前,耕地將於前期作物收穫後進行翻犁與曬田,以促進土壤風化、有機物分解及有毒物質釋放,而後將灌溉水引入耕地並保持2~4天後,於插秧前3天將第一肥料組合物施用於耕地的土壤中,以利後續栽培。
在本發明之低蛋白稻米的栽培方法100中,第一肥料組合物是作為基肥而施用於耕地的土壤中,且第一肥料組合物包含磷酸、氮與鉀,其中基於磷酸為1重量百分比,氮為2.5~4.5重量百分比,而鉀為1.5~3重量百分比。再者,耕地的有機質含量可為0.5%~3.5%,且耕地的pH值可為5.5~7.5,以提供水稻幼株優良的生長環境,但本發明並不以此為限。In the low-protein
步驟120為進行一插秧步驟,其係將一水稻幼株種植於耕地中。水稻幼株可為現行市面上或育苗中不同品種的水稻,且在插秧步驟中,水稻幼株將種植於水深約3公分的耕地中,以促進水稻幼株成活並於近地面處之基部節位的側芽長出分枝,以使水稻幼株進入分蘗期。
再者,水稻幼株可為一第一期作水稻或一第二期作水稻。詳細而言,現行之水稻栽培可為二期作,其係於同一耕地上一年種植作物二次之栽培制度,其中第一期作水稻是指2~6月栽培的水稻,而第二期作水稻是指7~10月栽培的水稻。當水稻幼株為第一期作水稻時,水稻幼株可為稻穀播種後18~20天且其苗齡為2.5~3.0葉的幼株,而當水稻幼株為第二期作水稻時,水稻幼株可為稻穀播種後10~12天且其苗齡為2.5~3.0葉的幼株,但本發明並不以此為限。Furthermore, the rice plantlet may be a first-crop rice plant or a second-crop rice plant. Specifically, the current rice cultivation may be a two-crop rice plant, which is a cultivation system in which crops are planted twice a year on the same cultivated land, wherein the first-crop rice plant refers to rice cultivated from February to June, and the second-crop rice plant refers to rice cultivated from July to October. When the rice plantlet is a first-crop rice plantlet, the rice plantlet may be a plantlet 18 to 20 days after rice sowing and having a seedling age of 2.5 to 3.0 leaves, and when the rice plantlet is a second-crop rice plantlet, the rice plantlet may be a plantlet 10 to 12 days after rice sowing and having a seedling age of 2.5 to 3.0 leaves, but the present invention is not limited thereto.
步驟130為進行一第一栽培步驟,其係將水稻幼株於耕地中栽培15~30天並施用一第二肥料組合物,以得一水稻成株。在第一栽培步驟中,水稻幼株是於水深約3~5公分的耕地中生長,以促進水稻幼株的根群發育與早期分蘗,而第二肥料組合物於第一栽培步驟中的施用次數為至少一次,換句話說,第二肥料組合物是於第一栽培步驟的15~30天中施用至少一次第二肥料組合物。再者,第二肥料組合物可以葉面施肥的方式施用於水稻幼株,以提升水稻幼株對於第二肥料組合物的利用效率,進而使水稻幼株發生一連串的分蘗,進而生長成為水稻成株。更精確地說,第一栽培步驟是在水稻幼株於主莖(main stem)上第五片葉子上出現葉領(collar)的早期分蘗階段時施用第二肥料組合物,而水稻幼株將會吸收第二肥料組合物的養分而繼續分蘗並生長為水稻成株。
在本發明之低蛋白稻米的栽培方法100中,第二肥料組合物包含鎂、錳、鐵、硼、鋅與銅,且基於銅為1重量百分比,鎂為6~18重量百分比,錳為2~6重量百分比,鐵為1.5~6重量百分比,硼為1~3重量百分比,鋅為2~5重量百分比。第二肥料組合物的成分非選用氮、磷酸與鉀等主要營養成分,而是以鎂、錳、鐵、硼、鋅、銅等微量元素所組成,以使水稻幼株有效利用前期施用之第一肥料組合物中的養分。In the
再者,當水稻幼株為第一期作水稻時,第一栽培步驟是將水稻幼株於耕地中栽培20~30天,而當水稻幼株為第二期作水稻時,第一栽培步驟則是將水稻幼株於耕地中栽培15~25天,但本發明並不以此為限。Furthermore, when the rice plants are first-stage rice plants, the first cultivation step is to cultivate the rice plants in the cultivated land for 20 to 30 days, and when the rice plants are second-stage rice plants, the first cultivation step is to cultivate the rice plants in the cultivated land for 15 to 25 days, but the present invention is not limited thereto.
另外,視耕地種類不同或栽種區域的氣候差異,第一栽培步驟亦可包含曬田期,曬田期是移除耕地中的灌溉水而使田土乾燥並略呈龜裂狀態,此時水稻幼株的根部將可獲得更為充足的氧氣,促使稻根向下生長,如此將有利於栽培後期吸收養分,並可防止倒伏,同時也可抑制無效分蘗,進而促進稻米產量及品質提升,但本發明並不以此為限。In addition, depending on the type of cultivated land or the climate difference in the planting area, the first cultivation step may also include a field sunning period. The field sunning period is to remove the irrigation water in the cultivated land to make the soil dry and slightly cracked. At this time, the roots of the young rice plants will be able to obtain more sufficient oxygen, which will promote the downward growth of the rice roots. This will be beneficial for absorbing nutrients in the later stage of cultivation and prevent lodging. At the same time, it can also inhibit ineffective tillering, thereby promoting the improvement of rice yield and quality, but the present invention is not limited to this.
步驟140為進行一第二栽培步驟,其係將水稻成株於耕地中栽培15~30天並施用一第三肥料組合物,以使水稻成株抽穗並完熟,且第三肥料組合物於第二栽培步驟中的施用次數為至少一次。詳細而言,水稻成株將於第二栽培步驟中開始進入繁殖期,此期間的耕地水深約為5~10公分,且水稻葉面的面積為全生育期中的最大值,以透過旺盛的光合作用而滋養稻穀,並配合在第二栽培步驟的15~30天中至少一次之第三肥料組合物的施用,使水稻成株進入繁殖期。更精確地說,第二栽培步驟是於水稻成株於主莖上第九片葉子上出現葉領後至第十片葉子上出現葉領前開始施用第三肥料組合物,此時水稻成株將開始出現圓錐花序(panicle)而進入繁殖期。而當第十片葉子上出現葉領後,水稻成株的圓錐花序將開始分化(differentiation)。
具體而言,在本發明之低蛋白稻米的栽培方法100中,第三肥料組合物包含鎂、錳、鐵、硼、鋅、銅、磷酸、氮與鉀,其中基於銅為1重量百分比,鎂為6~18重量百分比,錳為2~6重量百分比,鐵為1.5~6重量百分比,硼為1~3重量百分比,且鋅為2~5重量百分比。另外,磷酸於第三肥料組合物中的重量百分比為磷酸於第一肥料組合物中的重量百分比的1/20~1/50,氮於第三肥料組合物中的重量百分比為氮於第一肥料組合物中的重量百分比的1/20~1/50,且鉀於第三肥料組合物中的重量百分比為鉀於第一肥料組合物中的重量百分比的1/20~1/50。另外,第三肥料組合物可以葉面施肥的方式施用於水稻成株,以提升水稻成株對於第三肥料組合物的利用效率。Specifically, in the
再者,當水稻幼株為第一期作水稻時,第二栽培步驟是將水稻成株於耕地中栽培15~25天,而當水稻幼株為第二期作水稻時,第二栽培步驟是將水稻成株於耕地中栽培15~25天,但本發明並不以此為限。Furthermore, when the rice seedlings are first-stage rice, the second cultivation step is to cultivate the mature rice plants in the cultivated land for 15 to 25 days, and when the rice seedlings are second-stage rice, the second cultivation step is to cultivate the mature rice plants in the cultivated land for 15 to 25 days, but the present invention is not limited thereto.
步驟150為進行一收穫步驟,其係收穫水稻成株,以得複數個低蛋白稻米,其中所述之低蛋白稻米的蛋白質含量為每100公克的低蛋白稻米中包含小於6公克的蛋白質。
藉此,本發明之低蛋白稻米的栽培方法100透過在插秧步驟前先於耕地施用具有特定磷酸、氮與鉀之配比的第一肥料組合物作為基肥,並於水稻幼株種植於耕地後依序提供第二肥料組合物與第三肥料組合物的三階段營養差異栽培方式,可使栽培而得的低蛋白稻米具有較低的蛋白質含量,且每100公克的低蛋白稻米中僅包含小於6公克的蛋白質。因此,本發明之低蛋白稻米的栽培方法100所栽培而得之低蛋白稻米可應用於慢性腎臟病患者的飲食控制計畫中,並具有優異的市場應用潛力。Thus, the
[實施例與比較例][Examples and Comparative Examples]
本實驗是以台南16號的水稻幼株依據本發明之低蛋白稻米的栽培方法進行栽培,以得實施例1之低蛋白稻米,並進一步分析實施例1之低蛋白稻米的營養成分組成,以說明以本發明之低蛋白稻米的栽培方法100栽培而得之低蛋白稻米的蛋白質含量,而本發明之低蛋白稻米的栽培方法的詳細步驟及其細節請參低蛋白稻米的栽培方法100的說明,在此將不再贅述。In this experiment, rice seedlings from Tainan No. 16 were cultivated according to the cultivation method of low-protein rice of the present invention to obtain the low-protein rice of Example 1, and the nutritional composition of the low-protein rice of Example 1 was further analyzed to illustrate the protein content of the low-protein rice obtained by cultivating the low-protein
在本實驗中,本發明之第一肥料組合物是於插秧前預先施用於耕地的土壤中,並於不同栽培時期依序施用本發明之第二肥料組合物與本發明之第三肥料組合物。本實驗之水稻幼株可選用第一期作水稻或第二期作水稻,當水稻幼株為第一期作水稻時,第一栽培步驟係將水稻幼株於耕地中栽培20~30天,第二栽培步驟係將水稻成株於耕地中栽培15~25天,而當水稻幼株為第二期作水稻時,第一栽培步驟係將水稻幼株於耕地中栽培15~25天,第二栽培步驟係將水稻成株於耕地中栽培15~25天。In this experiment, the first fertilizer composition of the present invention is applied to the soil of the cultivated land before transplanting, and the second fertilizer composition of the present invention and the third fertilizer composition of the present invention are applied in sequence at different cultivation periods. The rice seedlings of this experiment can be selected as the first-stage rice or the second-stage rice. When the rice seedlings are the first-stage rice, the first cultivation step is to cultivate the rice seedlings in the cultivated land for 20 to 30 days, and the second cultivation step is to cultivate the rice mature plants in the cultivated land for 15 to 25 days. When the rice seedlings are the second-stage rice, the first cultivation step is to cultivate the rice seedlings in the cultivated land for 15 to 25 days, and the second cultivation step is to cultivate the rice mature plants in the cultivated land for 15 to 25 days.
在經過本發明之低蛋白稻米的栽培方法栽培後,水稻幼株將長成水稻成株,且水稻成株將抽穗並完熟,以收獲實施例1之低蛋白稻米。在營養成分組成分析方面是根據CNS5035食品中粗蛋白質之檢驗法(修訂日期:75年8月4日)來檢測實施例1之低蛋白稻米,以說明本發明之低蛋白稻米的栽培方法100所栽培而得之低蛋白稻米的蛋白質含量。再者,本實驗更同時檢測實施例1之低蛋白稻米的鉀含量,其中鉀含量分析方面是以衛生福利部103年8月25日部授食字第1031901169號公告修正重金屬檢驗方法總則(MOHWH0014.03)進行檢驗,以進一步說明本發明之低蛋白稻米的栽培方法100所栽培而得之低蛋白稻米應用於慢性腎臟病患者的飲食控制計畫的可行性。After being cultivated by the cultivation method of the low-protein rice of the present invention, the rice seedlings will grow into mature rice plants, and the mature rice plants will produce heading and mature, so as to harvest the low-protein rice of Example 1. The low-protein rice of Example 1 is tested in terms of nutritional composition according to CNS5035, the test method for crude protein in food (revised on August 4, 1986), so as to illustrate the protein content of the low-protein rice cultivated by the
另外,本實驗中另包含比較例1與比較例2,其中比較例1是市售之越光米,而比較例2是市售之梗米,且比較例1與比較例2的蛋白質含量與鉀含量是取自衛生福利部食品藥物管理署所公布之食品營養成分資料庫中越光米之食品營養成分平均值與梗米之食品營養成分平均值。In addition, this experiment also includes Comparative Example 1 and Comparative Example 2, wherein Comparative Example 1 is commercially available Koshihikari rice, and Comparative Example 2 is commercially available glutinous rice, and the protein content and potassium content of Comparative Example 1 and Comparative Example 2 are taken from the average nutritional value of Koshihikari rice and the average nutritional value of glutinous rice in the food nutritional composition database published by the Food and Drug Administration of the Ministry of Health and Welfare.
另外,本實驗中另包含一對照組,其係以習知的栽培方法栽培台南16號稻米植株,並觀察栽種而得的台南16號稻米的蛋白質含量差異。詳細而言,當對照組的稻米植株為第一期作水稻時,氮的施用總量為每公頃110~140公斤,磷酸的施用總量為每公頃40~60公斤,且鉀的施用總量為每公頃30~50公斤;而當對照組的稻米植株為第二期作水稻時,氮的施用總量為每公頃90~120公斤,磷酸的施用總量為每公頃30~40公斤,且鉀的施用總量為每公頃40~60公斤。In addition, this experiment also includes a control group, which is to cultivate Tainan No. 16 rice plants using a known cultivation method, and observe the difference in protein content of the cultivated Tainan No. 16 rice. Specifically, when the rice plants in the control group are the first-stage rice, the total amount of nitrogen applied is 110-140 kg per hectare, the total amount of phosphoric acid applied is 40-60 kg per hectare, and the total amount of potassium applied is 30-50 kg per hectare; and when the rice plants in the control group are the second-stage rice, the total amount of nitrogen applied is 90-120 kg per hectare, the total amount of phosphoric acid applied is 30-40 kg per hectare, and the total amount of potassium applied is 40-60 kg per hectare.
再者,對照組在整地步驟中係於耕地中施用包含氮、磷酸與鉀的基肥,其中氮的施用量為20%~30%的氮施用總量、磷酸的施用量為100%的磷酸施用總量,鉀的施用量為20%的鉀施用總量。而後,在插秧後10~15天係施用包含氮與鉀的第一次追肥,其中氮的施用量為20%的氮施用總量,鉀的施用量為30%的鉀施用總量。接著,在插秧後20~30天係施用包含氮與鉀的第二次追肥,其中氮的施用量為30%的氮施用總量,鉀的施用量為30%的鉀施用總量。再者,對照組更在插秧後45~70天(或幼穗長度為0.2~0.5公分時)施用20%~30%的氮施用總量與20%的鉀施用總量作為穗肥。在收穫而得對照組的台南16號稻米後,以CNS5035食品中粗蛋白質之檢驗法進行蛋白質含量檢測,以說明本發明之低蛋白稻米的栽培方法所栽培而得的低蛋白稻米與非以本發明之低蛋白稻米的栽培方法進行栽培而得之稻米以及市售稻米的蛋白質含量差異。Furthermore, in the control group, a basal fertilizer containing nitrogen, phosphoric acid and potassium was applied to the cultivated land during the land preparation step, wherein the nitrogen application amount was 20% to 30% of the total nitrogen application amount, the phosphoric acid application amount was 100% of the total phosphoric acid application amount, and the potassium application amount was 20% of the total potassium application amount. Then, 10 to 15 days after transplanting, a first topdressing containing nitrogen and potassium was applied, wherein the nitrogen application amount was 20% of the total nitrogen application amount, and the potassium application amount was 30% of the total potassium application amount. Then, 20 to 30 days after transplanting, a second topdressing containing nitrogen and potassium was applied, wherein the nitrogen application amount was 30% of the total nitrogen application amount, and the potassium application amount was 30% of the total potassium application amount. Furthermore, the control group was further fertilized with 20% to 30% of the total nitrogen application amount and 20% of the total potassium application amount as ear fertilizer 45 to 70 days after transplanting (or when the ear length was 0.2 to 0.5 cm). After the Tainan No. 16 rice of the control group was harvested, the protein content was tested using the CNS5035 crude protein test method in food to illustrate the difference in protein content between the low-protein rice cultivated by the cultivation method of the low-protein rice of the present invention, the rice cultivated by the cultivation method of the low-protein rice not of the present invention, and the commercially available rice.
請參照表一,其係呈現實施例1之低蛋白稻米、對照組的台南16號稻米、比較例1之越光米與比較例2之梗米的蛋白質含量分析結果。
如表一所示,在以100公克為單位時,實施例1之低蛋白稻米的蛋白質含量為4.2公克,明顯小於對照組之台南16號稻米的6.7公克、比較例1之越光米的7.6公克以及比較例2之梗米的7.0公克,顯示以本發明之低蛋白稻米的栽培方法所栽培而得之低蛋白稻米相比於市售產品具有較低的蛋白質含量。As shown in Table 1, when 100 grams is taken as the unit, the protein content of the low-protein rice of Example 1 is 4.2 grams, which is significantly lower than 6.7 grams of the Tainan No. 16 rice of the control group, 7.6 grams of the Koshihikari rice of Comparative Example 1, and 7.0 grams of the stalked rice of Comparative Example 2, indicating that the low-protein rice cultivated by the cultivation method of the low-protein rice of the present invention has a lower protein content than the commercially available products.
再請參照表二,其係呈現實施例1之低蛋白稻米、比較例1之越光米與比較例2之梗米的鉀含量分析結果。
如表二所示,在以100公克為單位時,實施例1之低蛋白稻米的鉀含量為77公克,小於比較例1之越光米的90公克以及比較例2之梗米的79公克,顯示以本發明之低蛋白稻米的栽培方法所栽培而得之低蛋白稻米不僅具有較低的蛋白質含量,亦具有較低的鉀含量,使得本發明之低蛋白稻米的栽培方法所栽培而得的低蛋白稻米可應用於慢性腎臟病患者的飲食控制計畫中,並具有優異的市場應用潛力。As shown in Table 2, the potassium content of the low-protein rice of Example 1 is 77 grams per 100 grams, which is less than 90 grams of the Koshihikari rice of Comparative Example 1 and 79 grams of the stalked rice of Comparative Example 2, indicating that the low-protein rice cultivated by the cultivation method of the low-protein rice of the present invention not only has a lower protein content, but also has a lower potassium content, so that the low-protein rice cultivated by the cultivation method of the low-protein rice of the present invention can be applied to the diet control plan of patients with chronic kidney disease and has excellent market application potential.
雖然本發明已以實施方式揭露如上,然其並非用以限定本發明,任何熟習此技藝者,在不脫離本發明之精神和範圍內,當可作各種之更動與潤飾,因此本發明之保護範圍當視後附之申請專利範圍所界定者為準。Although the present invention has been disclosed in the above embodiments, it is not intended to limit the present invention. Anyone skilled in the art can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the scope defined in the attached patent application.
100:低蛋白稻米的栽培方法 110,120,130,140,150:步驟100: Low-protein rice cultivation method 110,120,130,140,150: Steps
為讓本發明之上述和其他目的、特徵、優點與實施例能更明顯易懂,所附圖式之說明如下: 第1圖係繪示本發明之低蛋白稻米的栽培方法的步驟流程圖。 In order to make the above and other purposes, features, advantages and embodiments of the present invention more clearly understood, the attached drawings are described as follows: Figure 1 is a flow chart showing the steps of the cultivation method of low-protein rice of the present invention.
100:低蛋白稻米的栽培方法 100: Cultivation method of low-protein rice
110,120,130,140,150:步驟 110,120,130,140,150: Steps
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