TWI860571B - Biosensor measurement system and method thereof for detecting human lactic acid - Google Patents

Abstract

A biosensor measurement method for detecting human lactic acid (LA) includes: measuring a human lactic acid sample with a lactic acid biosensor member to obtain a lactic acid measurement signal; electrically connecting a lactic acid signal processing circuit with the lactic acid biosensor member; electrically processing the lactic acid measurement signal via the lactic acid signal processing circuit to obtain a processed lactic acid measurement signal; electrically connecting a signal readout circuit with the lactic acid signal processing circuit; and amplifying the processed lactic acid measurement signal via the signal readout circuit to obtain an amplified lactic acid measurement signal.

Description

Measurement system and method of biomedical sensor for lactate detection

The present invention relates to a measurement system and method of a biosensor for detecting lactic acid (lactic acid or lactase, lactate) in blood; in particular, to a measurement system and method of a biosensor for detecting lactic acid with a thermal compensation circuit; more particularly, to an extended-gate field effect transistor (EGFET) resistance-divider measurement system and method of a biosensor for detecting lactic acid; and more particularly, to a measurement system and method of a ruthenium dioxide thin-film biosensor for detecting lactic acid.

For example, a conventional vitamin C biosensor, such as the invention patent of the Republic of China Patent Publication No. TW-I374267 "Vitamin C sensor calibration measurement system and calibration method thereof", discloses a calibration measurement system for a vitamin C sensor, and the calibration measurement system for the vitamin C sensor includes a vitamin C sensor, a reference electrode, an amplifier, an analog-to-digital converter, and a programmable logic gate array module.

As mentioned above, the amplifier of the aforementioned TW-I374267 has a positive input terminal, a negative input terminal and an output terminal, and the reference electrode is selectively connected to the positive input terminal of the amplifier, and a sensing element is selectively connected to the negative input terminal of the amplifier, and the analog-to-digital converter is selectively connected to the output terminal of the amplifier.

As mentioned above, the programmable logic gate array module of the aforementioned TW-I374267 is selectively connected to the analog-to-digital converter, and the programmable logic gate array module includes a control chip, and the programmable logic gate array module further includes a controller, a multi-segment display and a light-emitting diode lamp, and the controller, the multi-segment display and the light-emitting diode lamp are respectively connected to the control chip.

Another commonly used biosensing element, such as the invention patent of the Republic of China Patent Publication No. TW-I377342 "Method for forming a sensing element of an extended gate field effect transistor and the sensing element formed thereby", discloses a sensing element of an extended gate field effect transistor, and the sensing element of the extended gate field effect transistor includes a substrate, a ruthenium-doped titanium dioxide sensing film and a wire.

As mentioned above, the ruthenium-doped titanium dioxide sensing film of the aforementioned TW-I377342 is formed on the substrate, and the ruthenium-doped titanium dioxide sensing film is a nanocrystalline surface, and the size of the nanocrystalline surface is about 37-61nm, and the wire extends from the ruthenium-doped titanium dioxide sensing film, which serves as an electrical contact to the outside.

As mentioned above, the sensing element of the extended gate field effect transistor of the aforementioned TW-I377342 may alternatively include a substrate, a titanium dioxide, a ruthenium-doped titanium dioxide or ruthenium oxide sensing film, a calcium ion sensing film and a conductor.

As mentioned above, the wire of the aforementioned TW-I377342 can alternatively extend from the titanium dioxide, ruthenium-doped titanium dioxide or ruthenium oxide sensing film as an external electrical contact, and the calcium ion sensing film is formed on the ruthenium-doped titanium dioxide or ruthenium oxide sensing film.

However, the aforementioned Vitamin C sensor of the Republic of China Patent Publication No. TW-I374267 and the sensing element of the extended gate field effect transistor of No. TW-I377342 do not disclose how to be used for blood lactate detection or related methods thereof, so there must be a need for how to be used for blood lactate detection or provide related methods thereof.

Another commonly used carbon nanotube biosensor is the invention patent of US Patent No. US-10031102 "Single-walled carbon nanotube biosensor for detection of glucose, lactate, and urea", which discloses a single-walled carbon nanotube biosensor for detecting blood glucose, lactate and urea, and the single-walled carbon nanotube biosensor is a micron scale multiplex biosensor based on single-walled carbon nanotubes.

As mentioned above, the single-walled carbon nanotube biosensor of the aforementioned US-10031102 is based on modification of a semiconductor single-walled carbon nanotube material and the use of a linker, which is non-covalently connected to the semiconductor single-walled carbon nanotube material and covalently coupled to an enzyme.

As mentioned above, the single-walled carbon nanotube biosensor of the aforementioned US-10031102 reacts with the enzyme on a biological substrate, and causes an increased resistance of one of the semiconductor single-walled carbon nanotube materials in the single-walled carbon nanotube biosensor.

However, the aforementioned single-walled carbon nanotube biosensor of US Patent No. US-10031102 only discloses that it can be used to detect lactate, and does not disclose how to enhance blood lactate detection or its related methods. Therefore, there must be a need for how to enhance blood lactate detection or provide its related methods.

In short, the aforementioned Republic of China Patent Publication No. TW-I374267, No. TW-I377342 and US Patent No. US-10031102 are only references to the technical background of the present invention and illustrate the current state of technical development, and are not intended to limit the scope of the present invention.

In view of this, in order to meet the above technical problems and needs, the present invention provides a measurement system and method of a biomedical sensor for lactate detection, which uses a lactate sensing element to measure a lactate measurement signal, and electrically connects the lactate sensing element to a lactate signal processing circuit, so that the lactate measurement signal is processed by the lactate signal processing circuit to obtain a processed lactate measurement signal, and the lactate signal processing circuit is electrically connected to a signal readout circuit, so that the processed lactate measurement signal is amplified by the signal readout circuit to obtain an amplified lactate measurement signal, so that compared with the conventional lactate sensing element, the purpose of providing accurate lactate measurement, improving sensing characteristics and measurement stability can be achieved.

The main purpose of the present invention is to provide a measurement system and method of a biomedical sensor for lactate detection, which utilizes a lactate sensing element to measure and obtain a lactate measurement signal, and electrically connects the lactate sensing element to a lactate signal processing circuit, so that the lactate measurement signal is processed by the lactate signal processing circuit to obtain a processed lactate measurement signal, and the lactate signal processing circuit is electrically connected to a signal readout circuit, so that the processed lactate measurement signal is amplified by the signal readout circuit to obtain an amplified lactate measurement signal, so as to achieve the effect of providing accurate measurement of lactate, improving sensing characteristics and measurement stability.

In order to achieve the above-mentioned purpose, the measurement method of the biomedical sensor for lactate detection in the preferred embodiment of the present invention includes:

Using a lactic acid sensing element to measure a lactic acid sample to be tested to obtain a lactic acid measurement signal;

Electrically connecting the lactate sensing element to a lactate signal processing circuit;

Processing the lactate measurement signal through the lactate signal processing circuit to obtain a processed lactate measurement signal;

Electrically connecting the lactate signal processing circuit to a signal readout circuit; and

The processed lactate measurement signal is amplified by the signal readout circuit to obtain an amplified lactate measurement signal.

The lactic acid sensing element of the preferred embodiment of the present invention is a ruthenium dioxide thin film lactic acid sensing element.

The lactate signal processing circuit of the preferred embodiment of the present invention includes an extended gate sensing field effect transistor and a voltage divider resistor so that the extended gate sensing field effect transistor forms a resistor, or the lactate signal processing circuit includes an NMOS extended gate sensing field effect transistor and a voltage divider resistor so that the NMOS extended gate sensing field effect transistor forms a resistor.

The signal reading circuit of the preferred embodiment of the present invention is connected to a temperature compensation circuit so that the processed lactate measurement signal is temperature compensated through the temperature compensation circuit to obtain a compensated lactate measurement signal.

The temperature compensation circuit of the preferred embodiment of the present invention has a predetermined temperature compensation range, and the predetermined temperature compensation range is between 25°C and 55°C.

The amplified lactate measurement signal of the preferred embodiment of the present invention is converted from an analog signal to a digital signal.

The lactate measurement signal of the preferred embodiment of the present invention is proportional to a lactate measurement concentration.

In order to achieve the above-mentioned purpose, the measurement system of the biomedical sensor for lactate detection in the preferred embodiment of the present invention includes:

A lactic acid sensing element, comprising a substrate;

A plurality of sensing electrodes are arranged on the substrate of the lactic acid sensing element, and the sensing electrodes are used to measure a lactic acid sample to be tested to obtain a lactic acid measurement signal;

At least one lactate signal processing circuit electrically connected to the lactate sensing element; and

At least one signal readout circuit electrically connected to the lactate signal processing circuit;

The lactate measurement signal is processed by the lactate signal processing circuit to obtain a processed lactate measurement signal, and then the processed lactate measurement signal is amplified by the signal readout circuit to obtain an amplified lactate measurement signal.

The lactic acid sensing element and substrate of the preferred embodiment of the present invention are combined with at least one microfluidic system so that the lactic acid sample to be tested can be measured through the microfluidic system.

The substrate of the preferred embodiment of the present invention is selected from a flexible substrate or a non-flexible substrate.

The plurality of sensing electrodes of the preferred embodiment of the present invention form an electrode array, and the electrode array includes a plurality of sensing electrode groups.

The lactate signal processing circuit of the preferred embodiment of the present invention includes an extended gate sensing field effect transistor and a voltage divider resistor, or the lactate signal processing circuit includes an NMOS extended gate sensing field effect transistor and a voltage divider resistor.

The signal reading circuit of the preferred embodiment of the present invention includes a positive voltage follower, a negative voltage follower and a differential amplifier.

1: Lactic acid sensing element

10: Microfluidic system

11: Substrate

12: Circuit layout layer

121: Reference electrode

122: Metal electrode layer

123: Sensing electrode

124: Sensing signal output electrode

13: Insulation pattern layer

100: Lactic acid sample to be tested

101: Microfluidic channel

2: Lactate signal processing circuit

21: Extended gate sensing field effect transistor

22: Voltage divider resistor

3: Signal reading circuit

31: Positive voltage follower

32: Negative voltage follower

33: Differential amplifier

4: Analog to digital conversion circuit

9: Lactate measurement signal has been amplified

90: Temperature compensation circuit

a: Epoxy resin layer

b: Aminopropyltriethoxysilane layer

c: First glutaraldehyde layer

d: Lactase layer

e: Second glutaraldehyde layer

Figure 1: A block diagram of a measurement system of a biomedical sensor for lactate detection according to the first preferred embodiment of the present invention.

Figure 2: Schematic diagram of the process of the measurement method of the biomedical sensor for lactate detection in the preferred embodiment of the present invention.

Figure 3: A schematic front view of a measurement system of a biomedical sensor for lactate detection using a lactate sensing element in a preferred embodiment of the present invention.

Figure 4: A schematic side view of a measurement system of a biomedical sensor for lactate detection using a lactate sensing element in a preferred embodiment of the present invention.

Figure 5: A schematic side view of a biomedical sensor measuring system for lactate detection using a lactate sensing element combined with a microfluidic system in accordance with the second preferred embodiment of the present invention.

Figure 6: Circuit diagram of a measurement system of a biomedical sensor for lactate detection using a lactate signal processing circuit in a preferred embodiment of the present invention.

Figure 7: Circuit diagram of a signal readout circuit used in the measurement system of a biomedical sensor for lactate detection in a preferred embodiment of the present invention.

Figure 8: Schematic diagram of the relationship between the response voltage and lactic acid concentration of the measurement system and method of the biomedical sensor for lactic acid detection in the preferred embodiment of the present invention.

Figure 9: A schematic diagram showing the relationship between the average response voltage and lactic acid concentration of the measurement system and method of the biomedical sensor for lactic acid detection in a preferred embodiment of the present invention.

Figure 10: A block diagram of a measurement system of a biomedical sensor for lactate detection according to the third preferred embodiment of the present invention.

Figure 11: A block diagram of a measurement system of a biomedical sensor for lactate detection according to the fourth preferred embodiment of the present invention.

In order to fully understand the present invention, the following will give examples of preferred embodiments and provide detailed descriptions with the accompanying drawings, which are not intended to limit the present invention.

The measurement system and method of the biomedical sensor for lactate detection of the preferred embodiment of the present invention are suitable for application in the measurement or comparison of various (blood) lactate biomedical sensing circuit components, the measurement or comparison of various (blood) lactate biomedical sensing chips, or the measurement of various (blood) lactate biomedical sensing systems and related measurement or comparison operations, but they are not intended to limit the scope of application of the present invention.

As mentioned above, the measurement system and method of the biomedical sensor for lactate detection in the preferred embodiment of the present invention are suitable for application in various blood delivery systems or methods thereof, various hemodialysis systems or methods thereof, or various extracorporeal blood circulation membrane lung support systems or methods thereof, but they are not intended to limit the scope of application of the present invention.

FIG. 1 is a block diagram of a measuring system of a biomedical sensor for lactate detection according to the first preferred embodiment of the present invention. Referring to FIG. 1, for example, the measuring system of a biomedical sensor for lactate detection according to the first preferred embodiment of the present invention mainly comprises a lactate sensing element 1, at least one or more lactate (measurement) signal processing circuits 2, and at least one or more signal readout circuits (i.e., lactate measurement signal readout circuits) 3, as shown on the left side of FIG. 1.

FIG. 2 is a schematic flow chart of a measurement method of a biomedical sensor for lactate detection according to a preferred embodiment of the present invention, which corresponds to the measurement system of the biomedical sensor for lactate detection according to FIG. Referring to FIG. 1 and FIG. 2, the measurement method of a biomedical sensor for lactate detection according to a preferred embodiment of the present invention includes step S1: For example, first, a response voltage (response voltage), such as a high level or a low level, is measured on a lactate sample 100 to be tested by using the lactate sensing element 1 (e.g., a _ruthenium dioxide (RuO 2 ) thin film lactate sensing element or other lactate sensing elements) to obtain a lactate measurement signal.

FIG. 3 discloses a front view schematic diagram of a lactic acid sensing element used in a measuring system of a biomedical sensor for lactic acid detection according to a preferred embodiment of the present invention. FIG. 4 discloses a side view schematic diagram of a lactic acid sensing element used in a measuring system of a biomedical sensor for lactic acid detection according to a preferred embodiment of the present invention, which corresponds to the front view schematic diagram of the lactic acid sensing element in FIG. 4.

Please refer to FIGS. 1, 2, 3 and 4 again. For example, the lactic acid sensing element 1 includes a substrate 11, a circuit layout layer (printed circuit layer) 12 and an insulating pattern layer (e.g., epoxy resin layer) 13. The substrate (e.g., polyethylene terephthalate or other flexible or non-flexible material) 11 is selected from a flexible substrate or a non-flexible substrate. The circuit layout layer 12 includes a plurality of reference electrodes (L-shaped patterns, which can be made of silver or other metal materials) 121, a plurality of metal electrode layers (sensing metal film layers) 122, a plurality of sensing electrodes (working electrodes, I-shaped patterns) 123 and a plurality of sensing signal output electrodes 124 for subsequent lactate measurement signal processing.

Please refer to Figures 1, 3 and 4 again. For example, the metal electrode layer 122 is made of ruthenium dioxide material or other materials, and the sensing electrode 123 is made of a composite material (e.g., composite lactase and silver nanoparticle material or other composite materials), and several sensing electrodes 123 form an electrode array or an electrode array sensing window, and the electrode array or electrode array sensing window includes several sensing electrode groups, so that multiple lactate measurement signals can be used for subsequent signal processing operations at the same time.

Please refer to Figures 1, 3 and 4 again. For example, the sensing electrode 123 further includes an epoxy resin layer a, an aminopropyl triethoxysilane layer (APTES layer, which can be selected as an enzyme fixing layer) b, a first glutaraldehyde layer (which can be selected as an enzyme fixing layer) c, a lactate enzyme layer (lactate layer, which can be fixed by cross-linking method) d and a second glutaraldehyde layer (which can be selected as an enzyme fixing layer) e, and the epoxy resin layer a, aminopropyl triethoxysilane layer b, first glutaraldehyde layer c, lactate enzyme layer d and second glutaraldehyde layer e are sequentially formed on the sensing electrode 123.

Please refer to Figures 1, 2, 3 and 4 again. The measurement method of the biomedical sensor for lactate detection of the preferred embodiment of the present invention includes step S2: For example, then, the lactate sensing element 1 (for example: the sensing signal output electrode 124) is electrically connected to the lactate signal processing circuit 2 or other related circuits by appropriate technical means to perform a lactate signal processing operation.

FIG. 5 shows a schematic side view of a biomedical sensor for lactate detection in the second preferred embodiment of the present invention, which uses a lactate sensing element combined with a microfluidic system. Referring to FIG. 5, relative to the first embodiment, the biomedical sensor for lactate detection in the second preferred embodiment of the present invention includes at least one microfluidic system 10, and the microfluidic system 10 can be selectively connected to at least one peripheral device (e.g., a fluid drive unit, an automatic control unit, or other functional units, not shown).

Please refer to Figures 1 and 5 again. For example, the microfluidic system 10 includes a plurality of microfluidic channels 101, so that the lactic acid sample 100 to be tested can flow through the plurality of microfluidic channels 101 respectively, and the lactic acid sensing element 1 and the substrate 11 are combined with the microfluidic system 10, so that the lactic acid sample 100 to be tested can be subjected to a measurement operation (e.g., different flow rate measurement or other measurement) through the plurality of microfluidic channels 101 of the microfluidic system 10.

FIG. 6 is a schematic circuit diagram showing a measurement system of a biomedical sensor for lactate detection using a lactate signal processing circuit according to a preferred embodiment of the present invention. Please refer to FIGS. 1 and 6 , for example, the lactate signal processing circuit 2 includes an extended gate sensing field effect transistor 21 (or an NMOS extended gate sensing field effect transistor can be selected) and a voltage divider resistor (for example, a variable resistor or a device with a variable resistor function can be selected) 22, so that the extended gate sensing field effect transistor 21 forms a resistor, and the lactate signal processing circuit 2 has a positive supply voltage V _DD , a negative supply voltage V _SS and an output voltage V _EG , and the output voltage V _EG is output from the voltage divider resistor 22. The preferred embodiment of the present invention can choose to use the following calculation formula for the output voltage V _EG :

Where _VDD is the positive supply voltage, _VSS is the negative supply voltage, _REGFET is the resistance formed by the extended gate sense field effect transistor and _RD is the voltage divider resistor.

Please refer to Figures 1, 3, 4 and 6 again. For example, the lactate measurement signal of the lactate sensing element 1 has a sensing voltage, and the sensing voltage can cause a change in a resistance value of the extended gate sensing field effect transistor 21 of the lactate signal processing circuit 2, thereby changing the output voltage V _EG generated by the voltage divider resistor 22.

Please refer to Figures 1, 2 and 6 again. The measurement method of the biomedical sensor for lactate detection of the preferred embodiment of the present invention includes step S3: for example, then, for example, then, the lactate measurement signal is appropriately processed by the lactate signal processing circuit 2 to obtain a processed lactate measurement signal, and the processed lactate measurement signal is appropriately output to one or more other circuits to appropriately perform other subsequent signal processing operations (for example: signal reading operation, signal amplification operation, signal correction operation or other signal processing operations).

Please refer to Figures 1, 2 and 6 again. The measurement method of the biomedical sensor for lactate detection of the preferred embodiment of the present invention includes step S4: using appropriate technical means to electrically connect one or more lactate signal processing circuits 2 to one or more signal readout circuits 3 to perform a signal amplification operation, as shown on the right side of Figure 1. In addition, the signal readout circuit 3 can reduce the noise interference of the processed lactate measurement signal (for example: noise filtering circuit).

FIG. 7 is a circuit diagram of a measurement system of a biomedical sensor for lactate detection according to a preferred embodiment of the present invention using a signal readout circuit. Referring to FIGS. 1 and 7, for example, the signal readout circuit 3 includes a positive voltage follower 31, a negative voltage follower 32, and a differential amplifier 33, and the signal readout circuit 3 has a positive input voltage V ₊ , a negative input voltage V- _, and an output voltage _VOUT , and the output voltage _VOUT is output from the differential amplifier 33 of the signal readout circuit 3, as shown on the right side of FIG. 7.

Please refer to Figures 1 and 7 again. For example, the positive voltage follower 31, the negative voltage follower 32 and the differential amplifier 33 are appropriately connected to form the signal readout circuit 3, and the positive voltage follower 31, the negative voltage follower 32 and the differential amplifier 33 are appropriately connected to form a two-stage operational amplifier device, and the lactic acid signal processing circuit 2 and the signal readout circuit 3 can be selectively integrated into a single chip or a single circuit device, and it can be selectively combined with at least one or more CMOS integrated circuits to improve its circuit characteristics.

Please refer to FIG. 7 again. For example, the positive voltage follower 31 includes a first operational amplifier and a resistor of the first follower (as shown on the lower left side of FIG. 7), and the negative voltage follower 32 includes a second operational amplifier and a resistor of the second follower (as shown on the upper left side of FIG. 7), and the differential amplifier 33 includes a third operational amplifier and four resistors of the differential amplifier (as shown on the right side of FIG. 7).

Please refer to Figures 1, 2 and 7 again. The measurement method of the biomedical sensor for lactate detection of the preferred embodiment of the present invention includes step S5: For example, then, the processed lactate measurement signal is appropriately read and amplified through one or more signal reading circuits 3 by appropriate technical means to obtain an amplified lactate measurement signal (i.e., an amplified response voltage) 9, and the amplified lactate measurement signal 9 can be appropriately output to the outside world (exterior) or converted (e.g.: analog-to-digital conversion).

FIG8 is a schematic diagram of the response voltage and lactate concentration of the measurement system and method of the biomedical sensor for lactate detection of the preferred embodiment of the present invention. Please refer to FIGS. 1, 2 and 8, for example, the lactate measurement signal is proportional to a lactate measurement concentration, and the lactate measurement concentration includes 0.2mM, 0.7mM, 1.3mM, 2mM, 3mM and 5mM. The linearity of the lactate sensing element 1, the lactate signal processing circuit 2 and the signal readout circuit 3 [LT1167] is 0.997, and the sensitivity of the lactate sensing element 1, the lactate signal processing circuit 2 and the signal readout circuit 3 is 53.41mV/mM, but it is not intended to limit the scope of application of the present invention.

FIG. 9 shows a schematic diagram of the average response voltage and lactate concentration of the measurement system and method of the biomedical sensor for lactate detection of the preferred embodiment of the present invention. Please refer to FIGS. 1, 2 and 9. For example, the linearity of the lactate sensing element [EGFET CSA] 1 and the lactate signal processing circuit 2 is 0.996, and the average sensitivity of the lactate signal processing circuit 2 is 32.93 mV/mM, but it is not intended to limit the scope of application of the present invention.

FIG. 10 is a block diagram of a measuring system of a biomedical sensor for lactate detection according to the third preferred embodiment of the present invention. Referring to FIG. 10, relative to the first embodiment, the measuring system of a biomedical sensor for lactate detection according to the third preferred embodiment of the present invention includes a temperature compensation circuit 90 to compensate for the ambient temperature of the lactate sensing element 1, the lactate signal processing circuit 2 or the signal readout circuit 3.

Please refer to FIG. 10 , for example, the signal readout circuit 3 can be electrically connected to the temperature compensation circuit 90 so as to perform temperature compensation on the processed lactate measurement signal through the temperature compensation circuit 90 to obtain a compensated lactate measurement signal, and the temperature compensation circuit 90 has a predetermined temperature compensation range, and the predetermined temperature compensation range is between 25°C and 55°C to reduce the temperature effect of the biomedical sensor.

FIG. 11 is a block diagram of a measurement system of a biomedical sensor for lactate detection according to the fourth preferred embodiment of the present invention. Referring to FIG. 11, relative to the first embodiment, the measurement system of a biomedical sensor for lactate detection according to the fourth preferred embodiment of the present invention includes an analog-to-digital conversion circuit 4, and the analog-to-digital conversion circuit 4 is used to convert the amplified lactate measurement signal 9 from an analog signal to a digital signal.

The above experimental data are preliminary experimental results obtained under specific conditions, which are only used to facilitate understanding or reference of the technical content of the present invention. Other related experiments are still required. The experimental data and its results are not used to limit the scope of rights of the present invention.

The above preferred embodiments are only examples to illustrate the present invention and its technical features. The technology of the embodiments can still be appropriately implemented in various substantially equivalent modifications and/or replacement methods; therefore, the scope of rights of the present invention shall be subject to the scope defined by the attached patent application scope. The copyright of this case is limited to the use of patent applications in the Republic of China.

1: Lactic acid sensing element

100: Lactic acid sample to be tested

2: Lactate signal processing circuit

3: Signal reading circuit

9: Lactate measurement signal has been amplified

Claims (10)

A measurement method of a biomedical sensor for lactate detection comprises: using a lactate sensing element to measure a lactate sample to be measured to obtain a lactate measurement signal; electrically connecting the lactate sensing element to a lactate signal processing circuit; processing the lactate measurement signal through the lactate signal processing circuit to obtain a processed lactate measurement signal; and processing the lactate signal to obtain a processed lactate measurement signal. The processing circuit is electrically connected to a signal readout circuit; and the processed lactate measurement signal is amplified through the signal readout circuit to obtain an amplified lactate measurement signal, wherein the lactate signal processing circuit includes an extended gate sensing field effect transistor and a voltage divider resistor, or the lactate signal processing circuit includes an NMOS extended gate sensing field effect transistor and a voltage divider resistor. According to the measurement method of the biomedical sensor for lactic acid detection described in Item 1 of the patent application scope, the lactic acid sensing element is a ruthenium dioxide film lactic acid sensing element. According to the measurement method of the biomedical sensor for lactate detection described in Item 1 of the patent application scope, the lactate sensing element and the substrate are combined with at least one microfluidic system so that the lactate sample to be tested can be measured through the microfluidic system. According to the measurement method of the biomedical sensor for lactate detection described in Item 1 of the patent application scope, the signal readout circuit is connected to a temperature compensation circuit so that the processed lactate measurement signal is temperature compensated through the temperature compensation circuit to obtain a compensated lactate measurement signal. According to the measurement method of the biomedical sensor for lactate detection described in Item 4 of the patent application, the temperature compensation circuit has a predetermined temperature compensation range, and the predetermined temperature compensation range is between 25°C and 55°C. A measurement system of a biomedical sensor for lactate detection, comprising: a lactate sensing element, comprising a substrate; a plurality of sensing electrodes, which are arranged on the substrate of the lactate sensing element, and the sensing electrodes are used to measure a lactate sample to be tested to obtain a lactate measurement signal; at least one lactate signal processing circuit, which is electrically connected to the lactate sensing element; and at least one signal readout circuit, which is electrically connected to the lactate signal processing circuit; The lactate measurement signal is processed by the lactate signal processing circuit to obtain a processed lactate measurement signal, and the processed lactate measurement signal is amplified by the signal readout circuit to obtain an amplified lactate measurement signal; wherein the lactate signal processing circuit includes an extended gate sensing field effect transistor and a voltage divider resistor, or the lactate signal processing circuit includes an NMOS extended gate sensing field effect transistor and a voltage divider resistor. According to the measurement system of the biomedical sensor for lactate detection described in Item 6 of the patent application scope, the lactate sensing element and the substrate are combined with at least one microfluidic system so that the lactate sample to be tested can be measured through the microfluidic system. According to the measurement system of the biomedical sensor for lactate detection described in Item 6 of the patent application scope, the plurality of sensing electrodes form an electrode array, and the electrode array includes a plurality of sensing electrode groups. According to the measurement system of the biomedical sensor for lactate detection described in Item 6 of the patent application scope, the signal readout circuit is connected to a temperature compensation circuit so that the processed lactate measurement signal is temperature compensated through the temperature compensation circuit to obtain a compensated lactate measurement signal. According to the measurement system of the biomedical sensor for lactate detection described in Item 6 of the patent application scope, the signal readout circuit includes a positive voltage follower, a negative voltage follower and a differential amplifier.

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