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TWI847199B - Observation carrier for microscope - Google Patents

Observation carrier for microscope Download PDF

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Publication number
TWI847199B
TWI847199B TW111128222A TW111128222A TWI847199B TW I847199 B TWI847199 B TW I847199B TW 111128222 A TW111128222 A TW 111128222A TW 111128222 A TW111128222 A TW 111128222A TW I847199 B TWI847199 B TW I847199B
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Taiwan
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rupture disk
base
chip
observation
section
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TW111128222A
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Chinese (zh)
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TW202331341A (en
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彭柏洋
梁竣傑
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邑流微測股份有限公司
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  • Sampling And Sample Adjustment (AREA)

Abstract

An observation carrier for microscope includes a stage body, a chip and a pressure relief assembly. The stage body includes a base and an upper cover, and the upper cover is detachably disposed on the base and includes a window. The chip is integrated on the upper cover and is adjacent to the base. An observation area is formed between the base and the chip. The observation area corresponds to the window and is suitable for carrying a sample to be tested. The pressure relief assembly is disposed on the base and includes a rupture disk, wherein the rupture disk connects to the observation area.

Description

顯微鏡觀測載台Microscope observation stage

本發明是有關於一種觀測載台,且特別是有關於一種顯微鏡觀測載台。The present invention relates to an observation platform, and in particular to a microscope observation platform.

光學顯微鏡及電子顯微鏡會藉由觀測載台承載待測樣品,以方便後續進行待測樣品的觀測。然而,觀測載台容易因為實驗參數設定不正確而使得待測樣品所在的內部腔體壓力過大,造成觀測載台的觀測視窗破裂及待測樣品的噴濺,並導致顯微鏡及觀測載台被汙染或損毀等問題。因此,如何改善觀測載台因為內部腔體壓力過大而造成觀測載台的觀測視窗破裂及待測樣品的噴濺是本領域的一大研究重點。Optical microscopes and electron microscopes carry samples to be tested on an observation stage to facilitate subsequent observation of the samples. However, due to incorrect experimental parameter settings, the observation stage is prone to excessive pressure in the internal cavity where the sample is located, causing the observation window of the observation stage to break and the sample to be tested to splash, and causing the microscope and the observation stage to be contaminated or damaged. Therefore, how to improve the observation stage's observation window breakage and sample to be splashed due to excessive pressure in the internal cavity is a major research focus in this field.

本發明提供一種顯微鏡觀測載台,可避免因觀測載台的內部壓力過大造成觀測載台的觀測視窗破裂及待測樣品的噴濺。The present invention provides a microscope observation platform, which can avoid the observation window of the observation platform from breaking and the sample to be tested from splashing due to excessive internal pressure of the observation platform.

本發明的顯微鏡觀測載台包括一載台主體、一晶片以及一洩壓組件。載台主體包括一底座及一上蓋,上蓋可拆卸地配置於底座上且包括一窗口。晶片整合於上蓋且鄰接於底座,底座與晶片之間形成一觀測區,觀測區對應窗口且適於承載一待測樣品。洩壓組件配置於底座且包括一破裂盤,其中破裂盤連通於觀測區。The microscope observation stage of the present invention includes a stage body, a chip and a pressure relief assembly. The stage body includes a base and an upper cover, the upper cover is detachably arranged on the base and includes a window. The chip is integrated with the upper cover and is adjacent to the base, and an observation area is formed between the base and the chip, the observation area corresponds to the window and is suitable for carrying a sample to be tested. The pressure relief assembly is arranged on the base and includes a rupture disk, wherein the rupture disk is connected to the observation area.

在本發明的一實施例中,上述的破裂盤的材質相同於晶片的材質,且破裂盤的厚度小於晶片的厚度。In one embodiment of the present invention, the material of the rupture disk is the same as that of the wafer, and the thickness of the rupture disk is less than the thickness of the wafer.

在本發明的一實施例中,上述的載台主體具有一氣流通道,氣流通道連通於破裂盤與觀測區之間。In one embodiment of the present invention, the carrier body has an air flow channel, which is connected between the rupture disk and the observation area.

在本發明的一實施例中,上述的氣流通道包括一第一區段及一第二區段,第一區段位於上蓋及底座之間且連接至觀測區,第二區段位於底座且連接於第一區段並延伸至破裂盤。In one embodiment of the present invention, the air flow channel includes a first section and a second section, the first section is located between the upper cover and the base and connected to the observation area, and the second section is located at the base and connected to the first section and extends to the rupture disk.

在本發明的一實施例中,上述的第一區段垂直於第二區段。In an embodiment of the present invention, the first section is perpendicular to the second section.

在本發明的一實施例中,上述的洩壓組件包括一接頭,接頭具有一通孔,破裂盤位於氣流通道及通孔之間,通孔連通外界。In one embodiment of the present invention, the pressure relief assembly includes a joint having a through hole, the rupture disc is located between the air flow channel and the through hole, and the through hole is connected to the outside.

在本發明的一實施例中,上述的顯微鏡觀測載台,包括一密封環,其中密封環配置於氣流通道及破裂盤之間。In one embodiment of the present invention, the microscope observation platform includes a sealing ring, wherein the sealing ring is disposed between the air flow channel and the rupture disk.

基於上述,在本發明的顯微鏡觀測載台中,破裂盤直接連通於觀測區。藉此,當承載待測樣品的觀測區的內部壓力過大時,破裂盤得以優先於對應觀測區的晶片進行破裂並洩壓,避免因晶片的破裂而導致待測樣品的噴濺,降低顯微鏡觀測載台被汙染或損毀的風險。Based on the above, in the microscope observation stage of the present invention, the rupture disk is directly connected to the observation area. Thus, when the internal pressure of the observation area carrying the sample to be tested is too large, the rupture disk can be ruptured and pressure released before the chip corresponding to the observation area, avoiding the splashing of the sample to be tested due to the rupture of the chip, and reducing the risk of contamination or damage to the microscope observation stage.

圖1是本發明一實施例的顯微鏡觀測載台的立體圖,圖2是圖1的顯微鏡觀測載台的局部剖視立體圖,圖3是圖1的顯微鏡觀測載台的剖面側視圖。Figure 1 is a three-dimensional view of a microscope observation platform of an embodiment of the present invention, Figure 2 is a partial cross-sectional three-dimensional view of the microscope observation platform of Figure 1, and Figure 3 is a cross-sectional side view of the microscope observation platform of Figure 1.

請參考圖1至圖3,本實施例的顯微鏡觀測載台100包括一載台主體110、一晶片120(圖3)及一洩壓組件130。載台主體110包括一底座111及一上蓋112。上蓋112可拆卸地配置於底座111上,且上蓋112包括一窗口113。晶片120整合於上蓋112且鄰接於底座111。底座111與晶片120之間形成一觀測區R(圖3),觀測區R對應窗口113且適於承載一待測樣品(未繪示)。洩壓組件130配置於底座111且包括一破裂盤131(圖3),破裂盤131連通於觀測區R。Please refer to Figures 1 to 3. The microscope observation stage 100 of this embodiment includes a stage body 110, a chip 120 (Figure 3) and a pressure relief assembly 130. The stage body 110 includes a base 111 and a cover 112. The cover 112 is detachably disposed on the base 111, and the cover 112 includes a window 113. The chip 120 is integrated with the cover 112 and is adjacent to the base 111. An observation area R (Figure 3) is formed between the base 111 and the chip 120. The observation area R corresponds to the window 113 and is suitable for carrying a sample to be tested (not shown). The pressure relief assembly 130 is disposed on the base 111 and includes a rupture disk 131 (Figure 3). The rupture disk 131 is connected to the observation area R.

藉此,本實施例的顯微鏡觀測載台100因為破裂盤131直接連通於觀測區R,當承載待測樣品的觀測區R的內部壓力過大時,破裂盤131得以優先於對應觀測區R的晶片120進行破裂並洩壓。也就是說,破裂盤131承受壓力的能力低於晶片120,從而在壓力達到足以使晶片120破裂的程度之前,破裂盤131會先破裂而洩壓,使壓力不再繼續上升,確保晶片120不會破裂。避免因晶片120的破裂而導致待測樣品的噴濺,降低顯微鏡觀測載台100被汙染或損毀的風險。Thus, the microscope observation stage 100 of this embodiment is directly connected to the observation area R because the rupture disk 131 is directly connected to the observation area R. When the internal pressure of the observation area R carrying the sample to be tested is too large, the rupture disk 131 can be ruptured and pressure released before the chip 120 corresponding to the observation area R. In other words, the rupture disk 131 has a lower ability to withstand pressure than the chip 120, so before the pressure reaches a level sufficient to rupture the chip 120, the rupture disk 131 will rupture and pressure release first, so that the pressure will no longer continue to rise, ensuring that the chip 120 will not rupture. This avoids the splashing of the sample to be tested due to the rupture of the chip 120, and reduces the risk of contamination or damage to the microscope observation stage 100.

在本實施例中,破裂盤131的材質相同於晶片120的材質,且破裂盤131的厚度小於晶片120的厚度,使破裂盤131得以優先於晶片120進行破裂。本實施例的晶片120的厚度為50奈米(nm),破裂盤131的厚度為30奈米(nm),但本發明不以此為限制。此外,在本發明的另一實施例中,破裂盤131的材質不同於晶片120的材質,且破裂盤131的厚度相同於晶片120的厚度,但破裂盤131的材料強度須低於晶片120的材料強度,使破裂盤131得以優先於晶片120進行破裂。本發明不對破裂盤131及晶片120的材質加以限制。In this embodiment, the material of the rupture disk 131 is the same as the material of the chip 120, and the thickness of the rupture disk 131 is less than the thickness of the chip 120, so that the rupture disk 131 can be broken before the chip 120. The thickness of the chip 120 in this embodiment is 50 nanometers (nm), and the thickness of the rupture disk 131 is 30 nanometers (nm), but the present invention is not limited to this. In addition, in another embodiment of the present invention, the material of the rupture disk 131 is different from the material of the chip 120, and the thickness of the rupture disk 131 is the same as the thickness of the chip 120, but the material strength of the rupture disk 131 must be lower than the material strength of the chip 120, so that the rupture disk 131 can be broken before the chip 120. The present invention does not limit the materials of the rupture disk 131 and the chip 120.

在本實施例中,載台主體110具有一氣流通道114(圖3),氣流通道114連通於破裂盤131與觀測區R之間,且氣流通道114包括一第一區段114A(圖3)及一第二區段114B(圖3)。第一區段114A位於上蓋112及底座111之間且由上蓋112及底座111共同形成。第一區段114A連接至觀測區R,第二區段114B位於底座111且連接於第一區段114A並延伸至破裂盤131。第一區段114A垂直於第二區段114B。In this embodiment, the carrier body 110 has an airflow channel 114 (FIG. 3), which is connected between the rupture disk 131 and the observation area R, and includes a first section 114A (FIG. 3) and a second section 114B (FIG. 3). The first section 114A is located between the upper cover 112 and the base 111 and is formed by the upper cover 112 and the base 111. The first section 114A is connected to the observation area R, and the second section 114B is located at the base 111 and connected to the first section 114A and extends to the rupture disk 131. The first section 114A is perpendicular to the second section 114B.

在本實施例中,洩壓組件130包括一接頭132,接頭132具有一通孔133,破裂盤131位於氣流通道114及通孔133之間,通孔133連通外界。當破裂盤131破裂時,觀測區R得以透過氣流通道114及通孔133連通於外界,避免晶片120因觀測區R的內部壓力過大而破裂。本實施例的通孔133在接頭132內呈一直線的設計,然而在本發明的其他實施例中,通孔133在接頭內可不為一直線的設計,本發明不對通孔133的形狀加以限制。In this embodiment, the pressure relief assembly 130 includes a connector 132, the connector 132 has a through hole 133, the rupture disk 131 is located between the airflow channel 114 and the through hole 133, and the through hole 133 is connected to the outside. When the rupture disk 131 is broken, the observation area R can be connected to the outside through the airflow channel 114 and the through hole 133, so as to prevent the chip 120 from being broken due to excessive internal pressure in the observation area R. The through hole 133 of this embodiment is designed to be a straight line in the connector 132, but in other embodiments of the present invention, the through hole 133 in the connector may not be a straight line design, and the present invention does not limit the shape of the through hole 133.

值得一提的是,本實施例的破裂盤131及接頭132配置於底座111的側面S(圖2)。然而,在本發明的其他實施例中,破裂盤131及接頭132可配置於底座111的頂面T(圖2),本發明不以此為限制。It is worth mentioning that the rupture disk 131 and the joint 132 of this embodiment are disposed on the side surface S ( FIG. 2 ) of the base 111. However, in other embodiments of the present invention, the rupture disk 131 and the joint 132 may be disposed on the top surface T ( FIG. 2 ) of the base 111, and the present invention is not limited thereto.

在本實施例中,顯微鏡觀測載台100包括一密封環140(圖3),密封環140配置於氣流通道114及破裂盤131之間,使觀測區R不會因氣流通道114及破裂盤131之間存有間隙而連通於外界,避免影響觀測區R在觀測時的壓力數值。In this embodiment, the microscope observation platform 100 includes a sealing ring 140 (Figure 3), which is arranged between the airflow channel 114 and the rupture disk 131, so that the observation area R will not be connected to the outside due to the gap between the airflow channel 114 and the rupture disk 131, thereby avoiding affecting the pressure value of the observation area R during observation.

在本實施例中,顯微鏡觀測載台100包括一液體容納組件150(圖3)及一頂撐件160(圖3)。液體容納組件150鄰接於晶片120,且液體容納組件150與晶片120之間形成觀測區R。頂撐件160配置於液體容納組件150與底座111之間,且頂撐件160適於抵頂液體容納組件150,有助於使液體容納組件150鄰接於晶片120。In this embodiment, the microscope observation stage 100 includes a liquid container assembly 150 (FIG. 3) and a top support 160 (FIG. 3). The liquid container assembly 150 is adjacent to the chip 120, and an observation area R is formed between the liquid container assembly 150 and the chip 120. The top support 160 is disposed between the liquid container assembly 150 and the base 111, and the top support 160 is suitable for supporting the liquid container assembly 150, which helps to make the liquid container assembly 150 adjacent to the chip 120.

此外,本實施例的顯微鏡觀測載台100包括一密封環170(圖3)及一密封環180,且底座111包括一底板1111及一罩體1112。底板1111與罩體1112相組裝以共同容納液體容納組件150及頂撐件160。密封環170配置於底板1111及罩體1112之間且圍繞液體容納組件150及頂撐件160,以加強底板1111及罩體1112之間的密封效果。密封環180配置於底座111的罩體1112與上蓋112之間且圍繞晶片120,以加強罩體1112與上蓋112之間的密封性。In addition, the microscope observation stage 100 of the present embodiment includes a sealing ring 170 (FIG. 3) and a sealing ring 180, and the base 111 includes a bottom plate 1111 and a cover 1112. The bottom plate 1111 and the cover 1112 are assembled to accommodate the liquid containing assembly 150 and the top support 160. The sealing ring 170 is disposed between the bottom plate 1111 and the cover 1112 and surrounds the liquid containing assembly 150 and the top support 160 to enhance the sealing effect between the bottom plate 1111 and the cover 1112. The sealing ring 180 is disposed between the cover 1112 and the upper cover 112 of the base 111 and surrounds the wafer 120 to enhance the sealing between the cover 1112 and the upper cover 112.

圖4是圖1的顯微鏡觀測載台的部分構件的立體圖。請參考圖3及圖4,本實施例的顯微鏡觀測載台100包括一旋蓋190(圖3)。旋蓋190具有至少一定位柱191(圖3),且底座111的罩體1112具有至少一定位槽1113(圖4)。旋蓋190的定位柱191適於隨著旋蓋190相對於底座111的旋轉而定位於罩體1112的定位槽1113,使旋蓋190可拆卸地配置於罩體1112上且將上蓋112限位於罩體1112上。FIG4 is a perspective view of some components of the microscope observation platform of FIG1. Referring to FIG3 and FIG4, the microscope observation platform 100 of this embodiment includes a screw cover 190 (FIG3). The screw cover 190 has at least one positioning column 191 (FIG3), and the cover body 1112 of the base 111 has at least one positioning groove 1113 (FIG4). The positioning column 191 of the screw cover 190 is suitable for being positioned in the positioning groove 1113 of the cover body 1112 as the screw cover 190 rotates relative to the base 111, so that the screw cover 190 can be detachably disposed on the cover body 1112 and the upper cover 112 is limited on the cover body 1112.

綜上所述,在本發明的顯微鏡觀測載台中,破裂盤透過氣流通道而直接連通於觀測區。藉此,當承載待測樣品的觀測區的內部壓力過大時,破裂盤得以優先於對應觀測區的晶片進行破裂並洩壓,避免因晶片的破裂而導致待測樣品的噴濺,降低顯微鏡觀測載台被汙染或損毀的風險。In summary, in the microscope observation stage of the present invention, the rupture disk is directly connected to the observation area through the air flow channel. Thus, when the internal pressure of the observation area carrying the sample to be tested is too high, the rupture disk can be ruptured and pressure released before the chip corresponding to the observation area, avoiding the splashing of the sample to be tested due to the rupture of the chip, and reducing the risk of contamination or damage to the microscope observation stage.

100:顯微鏡觀測載台 110:載台主體 111:底座 1111:底板 1112:罩體 1113:定位槽 112:上蓋 113:窗口 114:氣流通道 114A:第一區段 114B:第二區段 120:晶片 130:洩壓組件 131:破裂盤 132:接頭 133:通孔 140、170、180:密封環 150:液體容納組件 160:頂撐件 190:旋蓋 191:定位柱 R:觀測區 S:側面 T:頂面 100: microscope observation stage 110: stage body 111: base 1111: bottom plate 1112: cover 1113: positioning groove 112: upper cover 113: window 114: air flow channel 114A: first section 114B: second section 120: chip 130: pressure relief assembly 131: rupture disk 132: connector 133: through hole 140, 170, 180: sealing ring 150: liquid storage assembly 160: top support 190: screw cover 191: positioning column R: observation area S: side surface T: top surface

圖1是本發明一實施例的顯微鏡觀測載台的立體圖。 圖2是圖1的顯微鏡觀測載台的局部剖視立體圖。 圖3是圖1的顯微鏡觀測載台的剖面側視圖。 圖4是圖1的顯微鏡觀測載台的部分構件的立體圖。 FIG. 1 is a perspective view of a microscope observation stage of an embodiment of the present invention. FIG. 2 is a partial cross-sectional perspective view of the microscope observation stage of FIG. 1 . FIG. 3 is a cross-sectional side view of the microscope observation stage of FIG. 1 . FIG. 4 is a perspective view of some components of the microscope observation stage of FIG. 1 .

100:顯微鏡觀測載台 100: Microscope observation platform

110:載台主體 110: Carrier body

111:底座 111: Base

1111:底板 1111: Base plate

1112:罩體 1112: hood body

1113:定位槽 1113: Positioning slot

112:上蓋 112: Upper cover

113:窗口 113: Window

114:氣流通道 114: Air flow channel

114A:第一區段 114A: Section 1

114B:第二區段 114B: Second section

120:晶片 120: Chip

130:洩壓組件 130: Pressure relief assembly

131:破裂盤 131: Ruptured disc

132:接頭 132:Connection

133:通孔 133:Through hole

140、170、180:密封環 140, 170, 180: Sealing ring

150:液體容納組件 150: Liquid container assembly

160:頂撐件 160: Top support

190:旋蓋 190: Screw cap

191:定位柱 191: Positioning column

R:觀測區 R: Observation area

Claims (7)

一種顯微鏡觀測載台,包括:一載台主體,包括一底座及一上蓋,其中該上蓋可拆卸地配置於該底座上且包括一窗口;一晶片,整合於該上蓋且鄰接於該底座,其中該底座與該晶片之間形成一觀測區,該觀測區對應該窗口且適於承載一待測樣品;以及一洩壓組件,配置於該底座且包括一破裂盤,其中該破裂盤連通於該觀測區,其中該破裂盤承受壓力的能力低於該晶片承受壓力的能力,以使該觀測區的內部壓力過大時,該破裂盤優先於該晶片破裂。 A microscope observation platform comprises: a platform body, comprising a base and a cover, wherein the cover is detachably arranged on the base and comprises a window; a chip, integrated with the cover and adjacent to the base, wherein an observation area is formed between the base and the chip, the observation area corresponds to the window and is suitable for carrying a sample to be tested; and a pressure relief assembly, arranged on the base and comprising a rupture disk, wherein the rupture disk is connected to the observation area, wherein the pressure bearing capacity of the rupture disk is lower than the pressure bearing capacity of the chip, so that when the internal pressure of the observation area is too large, the rupture disk ruptures before the chip. 如請求項1所述的顯微鏡觀測載台,其中該破裂盤的材質相同於該晶片的材質,且該破裂盤的厚度小於該晶片的厚度。 A microscope observation stage as described in claim 1, wherein the material of the rupture disk is the same as the material of the chip, and the thickness of the rupture disk is less than the thickness of the chip. 如請求項1所述的顯微鏡觀測載台,其中該載台主體具有一氣流通道,該氣流通道連通於該破裂盤與該觀測區之間。 A microscope observation platform as described in claim 1, wherein the platform body has an air flow channel, and the air flow channel is connected between the rupture disk and the observation area. 如請求項3所述的顯微鏡觀測載台,其中該氣流通道包括一第一區段及一第二區段,該第一區段位於該上蓋及該底座之間且連接至該觀測區,該第二區段位於該底座且連接於該第一區段並延伸至該破裂盤。 The microscope observation platform as described in claim 3, wherein the airflow channel includes a first section and a second section, the first section is located between the upper cover and the base and connected to the observation area, and the second section is located on the base and connected to the first section and extends to the rupture disk. 如請求項4所述的顯微鏡觀測載台,其中該第一區段垂直於該第二區段。 A microscope observation platform as described in claim 4, wherein the first section is perpendicular to the second section. 如請求項3所述的顯微鏡觀測載台,其中該洩壓組件包括一接頭,該接頭具有一通孔,該破裂盤位於該氣流通道及該通孔之間,該通孔連通外界。 A microscope observation platform as described in claim 3, wherein the pressure relief assembly includes a joint, the joint has a through hole, the rupture disk is located between the air flow channel and the through hole, and the through hole is connected to the outside. 如請求項3所述的顯微鏡觀測載台,包括一密封環,其中該密封環配置於該氣流通道及該破裂盤之間。 The microscope observation platform as described in claim 3 includes a sealing ring, wherein the sealing ring is arranged between the airflow channel and the rupture disk.
TW111128222A 2022-01-26 2022-07-27 Observation carrier for microscope TWI847199B (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180353059A1 (en) * 2012-06-27 2018-12-13 Camplex, Inc. Optical assembly providing a surgical microscope view for a surgical visualization system
TWM580688U (en) * 2018-03-22 2019-07-11 邑流微測股份有限公司 microscope
CN110530780A (en) * 2019-08-30 2019-12-03 南通大学 A kind of cell hyperbaric chamber being equipped on microscopic system

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180353059A1 (en) * 2012-06-27 2018-12-13 Camplex, Inc. Optical assembly providing a surgical microscope view for a surgical visualization system
TWM580688U (en) * 2018-03-22 2019-07-11 邑流微測股份有限公司 microscope
CN110530780A (en) * 2019-08-30 2019-12-03 南通大学 A kind of cell hyperbaric chamber being equipped on microscopic system

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