TWI750489B - Use of fermented black soybeans for preparing anti-fatigue medicines - Google Patents

Abstract

The present invention provides the use of a black bean fermented product for preparing anti-fatigue medicines, wherein, the black bean fermented product is prepared by the following steps: step (a): providing a culture medium obtained by sterilizing black beans; step (b) ): inoculating a chicken leg mushroom into the medium for solid-state fermentation; and step (c): drying the solid-state fermented fermented product to obtain the black soybean fermented product.

Description

Use of black soybean fermented product for preparing anti-fatigue medicine

The present invention relates to a black bean fermented product, especially the use of a black bean fermented product for preparing anti-fatigue medicines.

Fatigue is not only a subjective feeling of discomfort, but also a physiological signal. In today's competitive industrial and commercial environment, various pressures make people prone to fatigue, burnout or even overwork, resulting in inability to concentrate and a decline in metabolism. Or depression and other symptoms, if there is no appropriate relief, it is easy to cause physical and mental trauma and thus affect the quality of life.

In order to improve the quality of life and competitiveness, a variety of anti-fatigue supplements have emerged. Among them, coffee is the most commonly used refreshing drink. However, coffee mainly stimulates the central nervous system through the caffeine contained in it, which excites the central nervous system and achieves a refreshing effect. However, long-term drinking will not only produce a sense of dependence, but also Stimulating the stomach can easily lead to hyperacidity. In addition, the mental state of the drinker after the refreshing effect subsides will be worse than before drinking, so it is not a suitable anti-fatigue drink.

Another common choice for anti-fatigue supplements is chicken essence, which is rich in total branched-chain amino acids (BCAAs) and small-molecule proteins, which have been shown to contribute to blood urea nitrogen, BUN) and the elimination of lactic acid (LA) in blood. Among them, urea nitrogen is a waste product generated after protein metabolism in the human body. When the concentration of urea nitrogen in the blood increases, it is easy to cause fatigue and fatigue; It is the main cause of muscle soreness. Therefore, drinking chicken essence can reduce the discomfort of fatigue and muscle soreness, so that the drinker can boost the spirit, achieve the effects of improving metabolism and anti-fatigue. However, the production of chicken essence requires continuous extraction of chickens at high temperature and high pressure to obtain a concentrate. Apart from the high cost of equipment and energy consumption required for the process, vegetarians cannot enjoy the benefits of chicken essence.

Therefore, the industry has yet to develop new anti-fatigue supplements that can be taken by all consumers to achieve anti-fatigue and refreshing effects.

In view of the problems faced by the above-mentioned prior art, the present invention provides the use of a black bean fermented product for preparing anti-fatigue medicines, wherein the black bean fermented product has good effects of anti-fatigue and alleviating muscle soreness.

In order to achieve the aforementioned purpose, the present invention provides the use of a black bean fermented product for preparing anti-fatigue medicines, wherein the black bean fermented product is prepared by the following steps: Step (a): providing a black bean fermented product after sterilization treatment. culture medium; step (b): inoculating a chicken leg mushroom in the culture medium for solid-state fermentation; and step (c): drying the solid-state fermented fermented product to obtain the black soybean fermented product; wherein, the chicken leg mushroom is obtained from The Food Industry Development Research Institute, a consortium, with deposit number BCRC 36658.

In the present invention, the black soybean is subjected to solid-state fermentation through the mushroom, and the obtained black soybean fermented product has the effect of reducing the concentration of urea nitrogen and lactic acid in the blood, thereby helping to reduce the discomfort of fatigue, fatigue and muscle soreness, so as to achieve anti-fatigue and Refreshing effect.

Preferably, the medium comprises: about 80-250 parts by weight of black beans, about 10-30 parts by weight of brown sugar, about 10-30 parts by weight of wheat, and about 5-25 parts by weight of black sesame.

The "black bean" in the present invention refers to the roots, leaves, stems, seeds or any combination or whole plant of black bean plants, preferably seeds. The seeds comprise seed coats, embryos and cotyledons, and can be used as a whole or in part or in combination. The black beans of the present invention are preferably unprocessed or processed samples such as drying, peeling, slicing or grinding. The variety of black beans is not particularly limited. In a preferred embodiment of the present invention, it is Manzhou black beans, Hengchun (Huangren) black beans, Huangren black beans, Qingren black beans, Danbo beans, black lentils or black beads Bean; more preferably, the black bean used is Hengchun black pearl bean, whose scientific name is Glycine max (L.).

The "chicken leg mushroom" of the present invention is any commercially available chicken leg mushroom. In a preferred embodiment of the present invention, its scientific name is Coprinus sp., more preferably, it can be obtained from the Food Industry Development Research Institute. BCRC 35671, 36031, 36055, 36074, 36075, 36076, 36077, 36078, 36100, 36101, 36102, 36103, 36104, 36302, 36317, 36790, 36391, 364 , 36597, 36649, 36658 and 36918 strains; BCRC 36658 is especially preferred.

Preferably, the medicinal product comprises the black soybean fermented product and a medically acceptable carrier.

Preferably, the anti-fatigue system includes reducing the blood urea nitrogen concentration after exercise.

Preferably, the anti-fatigue method includes reducing the concentration of lactic acid in blood after exercise.

Preferably, the effective dose of the black bean fermented product contained in the medicinal product in the human body is 75 mg/kg to 450 mg/kg; wherein, the BCAA content contained in each gram of the black bean fermented product is 47 mg to 60 mg.

Preferably, the medicinal product is ordered to be administered at least once a day.

As used herein, the term "carrier" or "medically acceptable carrier" refers to a diluent, excipient, acceptor or the like, which is well known to those of ordinary skill in the art of manufacturing pharmaceutical products.

The dosage of the medicinal product can be determined by the relevant medical personnel according to the common knowledge in the technical field according to factors such as the patient's age, weight, health condition, disease type, disease progression, and affected part. The pharmaceutical composition in this case can also be administered alone or in combination with other pharmaceuticals.

To sum up, the black bean fermented product obtained by inoculating the black bean as a culture medium and inoculating the chicken leg mushroom for solid-state fermentation has the effects of reducing fatigue and alleviating muscle soreness, so it can be further applied to the preparation of anti-fatigue medicines.

In the description, the range represented by "a decimal value to a larger value", unless otherwise specified, means that the range is greater than or equal to the decimal value to less than or equal to the larger value. For example: 80 to 250 parts by weight, which means that the range is "greater than or equal to 80 parts by weight and less than or equal to 250 parts by weight".

The following embodiments are used as examples to illustrate the preparation method of the black soybean fermented product of the present invention and its use in preparing anti-fatigue medicines. Those skilled in the art can easily understand the advantages and disadvantages of the present invention through the content of the present invention. effect, and various modifications and changes can be made without departing from the spirit of the present invention to implement or apply the content of the present invention.

Example 1 : Black bean fermented product

Get 155 grams of Hengchun black pearl beans, 20 grams of brown sugar, 20 grams of wheat and 5 grams of black sesame and mix them evenly as medium ingredients, add water, and make the addition ratio between the medium ingredients and water be 150 weight volume percent (w/v %), then raise the temperature to 121 °C, hold the temperature for at least 30 minutes to be sterilized and then become a culture medium.

Then the mushroom BCRC 36658 is inoculated in the aforementioned substratum, to carry out solid-state fermentation, the substratum is mixed with the mushroom culture liquid of the mushroom at a ratio of 10 volume weight percent (v/w%), and the bacterial species concentration is about 10 g biomass g/ The ratio of L was adjusted, stirred evenly, and incubated at 25°C for 30 days.

After the fermentation is completed, the fermented product is dried at a temperature of about 50° C. and a pressure of about 0.5 kg/cm ² to obtain the black soybean fermented product of Example 1.

Test Example: Anti-fatigue Test

By feeding male rats of Wistar strain (purchased from Lesco Biotechnology Co., Ltd.) containing different concentrations of the black bean fermented product of Example 1, the blood lactic acid (LA) and blood urea nitrogen were measured before and after swimming. (blood urea nitrogen, BUN) and other concentrations, swimming time and liver sugar content, and compared with the positive control group fed with commercial ginseng chicken essence and the control group fed without active ingredients to evaluate the effect of each test article on resistance The effect of exercise fatigue. The components of each test product are listed in Table 1 below. Table 1: Control group, the dosage of the black soybean fermented product of Example 1 contained in Examples 1A to 1C, and the composition of the positive control group control group Example 1A Example 1B Example 1C Positive control group Content of Example 1*(mg/kg) 0 740 1480 3700 Commercially available ginseng chicken essence *(mg/kg): How many milligrams of the black soybean fermented product of Example 1 are administered per kilogram of rat

Specifically, after feeding the rats for 4 weeks according to the conditions of the aforementioned groups, the rats were placed in a water tank with a diameter of 15 cm, a water depth of 20 cm, and a water temperature of 27±1 °C, and the rats were forced to swim and struggle. Until the physical strength is exhausted and sinking, the time from falling into the water to the nostril sinking into the water is the swimming time; Swimming in water with a temperature of 30°C for 10 minutes and then stopping, and blood was collected before and after swimming to determine the LA value and BUN value in the blood of the rats; The liver was sacrificed and its concentration was measured by decomposing liver sugar into glucose. The results of the aforementioned experiments to evaluate the efficacy against exercise fatigue are listed in Table 2 below. Table 2: Results of measuring LA value, BUN value, swimming time, and glycogen content after 4 weeks of feeding control group, Examples 1A to 1C and positive control group BUN value before swimming (mg/dl) BUN value after swimming (mg/dl) LA change rate before and after swimming (%) Swimming time (min) Glucose content (mg/g) control group 16.3±0.6 21.4±0.6 0.8±0.4 51.7±6.5 0.33±0.02 Positive control group 17.4±0.6 22.3±0.9 -11.3±2.9 54.0±2.6 0.54±0.01 Example 1A 17.0±1.9 17.0±0.5 -9.3±7.5 56.0±4.7 0.49±0.04 Example 1B 15.9±0.4 17.6±0.3 -15.9±4.9 58.0±4.9 0.74±0.03 Example 1C 15.2±0.5 18.9±0.9 -35.9±7.7 65.8±3.8 1.19±0.16

As can be seen from the measurement results in Table 2 above, the BUN values of each group have no significant difference before exercise, while feeding Example 1A, Example 1B, and Example 1C (the black soybean fermented content of Example 1 is low, medium, and high) The BUN values of the rats after exercise were significantly lower than those of the control group and the positive control group, indicating that feeding the rats containing the black bean fermented product of Example 1 can effectively reduce the concentration of urea nitrogen in the blood after exercise (that is, reduce the blood urea nitrogen concentration). The concentration of waste products after protein metabolism), among which the BUN value of Example 1A group after exercise is the lowest and has the best effect.

According to the experimental results of the rate of change of LA in the blood of rats before and after exercise, the groups of Examples 1A to 1C have the effect of reducing the accumulation of lactic acid compared with the control group. In addition, the groups of Example 1A and Example 1B reduce lactic acid. The accumulation effect is comparable to that of the positive control group, and the group of Example 1C has an excellent effect of reducing the accumulation of lactic acid, even about three times that of the positive control group. From the experimental results of the aforementioned biochemical indicators, it can be seen that feeding rats the test product containing the black soybean fermented product of Example 1 has the effect of resisting fatigue and alleviating muscle soreness.

Then through the test result of swimming time, it can be known that the swimming time of the groups of Examples 1A to 1C is longer than that of the control group and the positive control group, which means that the black bean fermented product of Example 1 with low, medium and high content can be specifically fed. Compared with the positive control group, the group of Example 1C can prolong the swimming time of the rats to about 10 minutes, which has a very excellent effect.

Finally, according to the determination of hepatic glucose content, the groups of Examples 1A to 1C all have higher hepatic glucose contents than the control group, while the hepatic glucose contents of the groups of Example 1B and Example 1C are higher than those of the positive control group Group. By accumulating more glycogen in the liver, it can supply the energy needed by the rat during exercise for a longer time, thereby increasing the exercise endurance of the rat, and avoiding fatigue due to lack of energy.

To sum up, the black bean fermented product obtained by solid-state fermentation of Coprinus jelly using black bean as a culture medium in the present invention has been proved by experiments to have the effects of anti-fatigue, alleviating muscle soreness and even improving exercise endurance, so it can be further applied to the preparation of anti-fatigue medicines. product.

The above-mentioned embodiments are only examples to illustrate the present invention, and are not intended to limit the scope of the application rights claimed in this creation in any respect. Those with ordinary knowledge in the technical field can make corresponding adjustments according to the spirit of this creation. The scope of the rights claimed in the present invention should be based on the scope of the patent application, rather than being limited to the above-mentioned specific embodiments.

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Claims (6)

The use of a black bean fermented product for preparing anti-fatigue medicines, wherein the black bean fermented product is made from the following steps: step (a): providing a culture medium formed by sterilizing black beans; step (b): Inoculate a chicken leg mushroom into the medium to carry out solid-state fermentation; and step (c): drying the fermented product through solid-state fermentation to obtain the black soybean fermented product; wherein, the chicken leg mushroom is obtained from the Food Industry Development Research Institute of a consortium corporation , and the deposit number is BCRC 36658; the scientific name of the black bean is Glycine max (L.). The use according to claim 1, wherein the medicinal product comprises the black soybean fermented product and a medically acceptable carrier. The use according to claim 1, wherein the anti-fatigue comprises reducing blood urea nitrogen concentration after exercise. The use according to claim 1, wherein the anti-fatigue comprises reducing blood lactate concentration after exercise. The use according to claim 1, wherein the effective dose of the black soybean fermented product is 75 mg/kg to 450 mg/kg. The use as claimed in claim 1, wherein the medicinal product is customarily administered at least once a day.