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TWI679209B - 使用陽離子交換層析法純化同功抗體之方法 - Google Patents

使用陽離子交換層析法純化同功抗體之方法 Download PDF

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Publication number
TWI679209B
TWI679209B TW107112953A TW107112953A TWI679209B TW I679209 B TWI679209 B TW I679209B TW 107112953 A TW107112953 A TW 107112953A TW 107112953 A TW107112953 A TW 107112953A TW I679209 B TWI679209 B TW I679209B
Authority
TW
Taiwan
Prior art keywords
antibody
buffer
fragment
exchange chromatography
cation exchange
Prior art date
Application number
TW107112953A
Other languages
English (en)
Chinese (zh)
Other versions
TW201841933A (zh
Inventor
姜成默
Sung-Muk KANG
李弘宰
Hong Jai Lee
金炅嬅
Kyunghwa Kim
柳貞敏
Jung Min Yoo
李庭旼
Chung Min Lee
李京和
Gyung-Hwa LEE
李東億
Dong Eok Lee
林聖仁
Sung In Lim
河敬植
Gyong-sik HA
Original Assignee
南韓商Ｃｊ醫藥健康股份有限公司
Cj Healthcare Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 南韓商Ｃｊ醫藥健康股份有限公司, Cj Healthcare Corporation filed Critical 南韓商Ｃｊ醫藥健康股份有限公司
Publication of TW201841933A publication Critical patent/TW201841933A/zh
Application granted granted Critical
Publication of TWI679209B publication Critical patent/TWI679209B/zh

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/522—CH1 domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2318/00—Antibody mimetics or scaffolds

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Peptides Or Proteins (AREA)
TW107112953A 2017-04-14 2018-04-13 使用陽離子交換層析法純化同功抗體之方法 TWI679209B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20170048635 2017-04-14
??10-2017-0048635 2017-04-14
KR10-2017-0048635 2017-04-14

Publications (2)

Publication Number Publication Date
TW201841933A TW201841933A (zh) 2018-12-01
TWI679209B true TWI679209B (zh) 2019-12-11

Family

ID=63792713

Family Applications (1)

Application Number Title Priority Date Filing Date
TW107112953A TWI679209B (zh) 2017-04-14 2018-04-13 使用陽離子交換層析法純化同功抗體之方法

Country Status (4)

Country Link
KR (1) KR102140693B1 (fr)
AR (1) AR111465A1 (fr)
TW (1) TWI679209B (fr)
WO (1) WO2018190677A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102209790B1 (ko) * 2018-12-20 2021-02-01 에이치케이이노엔 주식회사 친화성 크로마토그래피를 이용한 백신용 바이러스 정제방법
CN110066314B (zh) * 2019-04-01 2023-10-27 上海药明生物技术有限公司 一种高效提高多聚体分离分辨率的亲和纯化工艺
CN114539417A (zh) * 2020-11-26 2022-05-27 盛禾(中国)生物制药有限公司 一种有效去除双特异性抗体同源二聚体的层析纯化工艺

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110206687A1 (en) * 2009-10-20 2011-08-25 Abbott Laboratories Isolation And Purification Of Anti-IL-13 Antibodies Using Protein A Affinity Chromatography

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090148435A1 (en) * 2007-10-30 2009-06-11 Genentech, Inc. Antibody purification by cation exchange chromatography
EP2655412B1 (fr) * 2010-12-21 2018-01-17 F. Hoffmann-La Roche AG Préparation d'anticorps enrichie en isoformes et son procédé d'obtention
KR101460266B1 (ko) * 2012-06-05 2014-11-11 씨제이헬스케어 주식회사 신규한 지속형 인간 성장호르몬의 정제 방법
KR101569783B1 (ko) * 2013-06-05 2015-11-19 한화케미칼 주식회사 항체의 정제 방법
US10351592B2 (en) * 2013-10-30 2019-07-16 Celltrion, Inc. Method for separating antibody isoforms using cation exchange chromatography

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110206687A1 (en) * 2009-10-20 2011-08-25 Abbott Laboratories Isolation And Purification Of Anti-IL-13 Antibodies Using Protein A Affinity Chromatography

Also Published As

Publication number Publication date
AR111465A1 (es) 2019-07-17
WO2018190677A2 (fr) 2018-10-18
TW201841933A (zh) 2018-12-01
WO2018190677A3 (fr) 2019-01-17
KR102140693B1 (ko) 2020-08-05
KR20180116159A (ko) 2018-10-24

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