TWI656845B - Method for manufacturing noodles, method for loosening and improving surface noodles Loosen the modifier - Google Patents
Method for manufacturing noodles, method for loosening and improving surface noodles Loosen the modifier Download PDFInfo
- Publication number
- TWI656845B TWI656845B TW104130362A TW104130362A TWI656845B TW I656845 B TWI656845 B TW I656845B TW 104130362 A TW104130362 A TW 104130362A TW 104130362 A TW104130362 A TW 104130362A TW I656845 B TWI656845 B TW I656845B
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- Taiwan
- Prior art keywords
- lipase
- amylase
- noodles
- water
- noodle
- Prior art date
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- 125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/109—Types of pasta, e.g. macaroni or noodles
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
- A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
- A21D2/08—Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
- A21D2/36—Vegetable material
- A21D2/362—Leguminous plants
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
- A21D8/00—Methods for preparing or baking dough
- A21D8/02—Methods for preparing dough; Treating dough prior to baking
- A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
- A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/50—Polysaccharides, gums
- A23V2250/51—Polysaccharide
- A23V2250/5108—Cellulose
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Noodles (AREA)
Abstract
本發明的目的在於提供一種於保存中抑制鬆開劣化等品質降低、且有效率地製造美味的麵類的方法以及麵類的鬆開改良劑。本發明藉由將脂肪酶中具有1,3-位置特異性的脂肪酶及澱粉酶添加至麵中,可驚人地改良保存中的鬆開劣化,藉由進一步併用水溶性半纖維素,可進一步改良麵的鬆開。 An object of the present invention is to provide a method and a surface release improving agent which are capable of efficiently producing a delicious noodle such as a reduction in quality such as release deterioration during storage. In the present invention, by adding a lipase having a 1,3-position specificity to a lipase and an amylase to the surface, the loosening deterioration during storage can be surprisingly improved, and further, by using water-soluble hemicellulose in combination, further The loosening of the improved surface.
Description
本發明是有關於一種麵類的製造方法及麵用鬆開改良劑。 The present invention relates to a method for producing a noodle and a surface release improving agent.
由於近年來的食品的多樣化,加工食品的需求(needs)不斷增大。尤其對於麵類而言,大量經加工、調理的產品於便利店(convenience store)、超級市場(super market)等中銷售,被許多消費者所食用。 Due to the diversification of food in recent years, the demand for processed foods has increased. Especially for noodles, a large number of processed and conditioned products are sold in convenience stores, super markets, etc., and are consumed by many consumers.
對於該些流通、市售的加工食品而言,尤其成問題的是加工的便利性及由時間經過所致的品質劣化。麵類通常含有澱粉,於加工、調理時澱粉質流出至食品表面,導致食品彼此黏著。結果,因麵彼此的附著而導致難以細分為一餐分量,或難以於食用時與汁液或醬料(sauce)混合。速食麵的情況下,麵黏著的部分難以進行熱水回沖。此種問題導致生產效率的降低、成本上漲(cost up),進而導致如下結果:即便是已製成的食品,於食用時也難吃、不美味。 Particularly problematic for these distributed and commercially available processed foods is the convenience of processing and quality deterioration due to passage of time. The noodles usually contain starch, which flows out to the surface of the food during processing and conditioning, causing the food to stick to each other. As a result, it is difficult to subdivide into a meal component due to the adhesion of the faces to each other, or it is difficult to mix with juice or sauce when eating. In the case of instant noodles, it is difficult for the surface to adhere to the hot water back. Such problems lead to lower production efficiency and cost up, which in turn leads to the following results: even foods that have been made are unpalatable and unpalatable when eaten.
作為消除該食品彼此的黏著性、改善鬆開性的先前方法,已知於麵條中添加具有鬆開功能的液體的方法。例如有混合油脂或乳化油脂的方法(專利文獻1),但若將油脂用於鬆開劑, 則食用時於湯汁上產生浮油,無法用於麵線或蕎麥、烏龍麵般的麵類。除此以外,有添加親水親脂平衡值(Hydrophilic Lipophilic Balance,HLB)高的蔗糖脂肪酸酯的方法(專利文獻2)、添加有機酸的方法(專利文獻3)、一面施加機械振動一面進行加工的方法、使用水溶性半纖維素的方法(專利文獻4)等,但均無法獲得充分的鬆開性,且單獨使用乳化劑的情況下有口感劣化等問題。 As a prior method for eliminating the adhesion between the foods and improving the looseness, a method of adding a liquid having a loosening function to the noodles is known. For example, there is a method of mixing fats and oils or emulsified fats and oils (Patent Document 1), but if fats and oils are used for the release agent, When it is eaten, it produces oil slick on the soup, which cannot be used for noodles or buckwheat or udon noodles. In addition, there is a method of adding a sucrose fatty acid ester having a high hydrophilic lipophilic balance (HLB) (Patent Document 2), a method of adding an organic acid (Patent Document 3), and processing while applying mechanical vibration. In the method of using water-soluble hemicellulose (Patent Document 4), etc., it is not possible to obtain sufficient release property, and when an emulsifier is used alone, there is a problem that the texture is deteriorated.
先前以來,已知於麵的麵糰(noodle dough)中添加酵素的方法。例如專利文獻5~專利文獻9中記載有將脂肪酶添加至麵的麵糰中的方法。該些方法均確認到了改善口感或鬆開的效果,但該效果不充分。 Previously, a method of adding an enzyme to a noodle dough was known. For example, Patent Document 5 to Patent Document 9 describe a method of adding lipase to a dough of a dough. All of these methods confirmed the effect of improving the mouthfeel or loosening, but the effect was insufficient.
關於澱粉酶的添加,亦於專利文獻10中有記載,但鬆開性的效果不充分,而且該方法為對經α化的麵條進行澱粉酶處理、進而藉由加熱而失活的方法,有缺乏簡便性的問題。 The addition of the amylase is also described in Patent Document 10, but the effect of the release property is insufficient, and the method is a method of subjecting the gelatinized noodles to amylase treatment and deactivation by heating. Lack of simplicity.
[現有技術文獻] [Prior Art Literature]
[專利文獻] [Patent Literature]
[專利文獻1]日本專利特開平3-175940號公報 [Patent Document 1] Japanese Patent Laid-Open No. Hei 3-175940
[專利文獻2]日本專利特公昭60-8103號公報 [Patent Document 2] Japanese Patent Publication No. Sho 60-8103
[專利文獻3]日本專利特開昭61-181350號公報 [Patent Document 3] Japanese Patent Laid-Open No. 61-181350
[專利文獻4]日本專利特開平6-121647號公報 [Patent Document 4] Japanese Patent Laid-Open No. Hei 6-121647
[專利文獻5]日本專利特開平5-292908號公報 [Patent Document 5] Japanese Patent Laid-Open No. Hei 5-292908
[專利文獻6]日本專利特開平6-113771號公報 [Patent Document 6] Japanese Patent Laid-Open No. Hei 6-113771
[專利文獻7]日本專利特開平9-70269號公報 [Patent Document 7] Japanese Patent Laid-Open No. Hei 9-70269
[專利文獻8]日本專利特開2001-224299號公報 [Patent Document 8] Japanese Patent Laid-Open Publication No. 2001-224299
[專利文獻9]日本專利特開2001-327257號公報 [Patent Document 9] Japanese Patent Laid-Open Publication No. 2001-327257
[專利文獻10]日本專利特開平07-123943號公報 [Patent Document 10] Japanese Patent Laid-Open No. Hei 07-123943
本發明的目的在於提供一種於保存中抑制鬆開劣化等品質降低、且有效率地製造美味的麵類的方法以及麵類的鬆開改良劑。 An object of the present invention is to provide a method and a surface release improving agent which are capable of efficiently producing a delicious noodle such as a reduction in quality such as release deterioration during storage.
本發明者等人鑒於所述課題而反覆進行了努力研究,結果發現,藉由將具有1,3-位置特異性的脂肪酶及澱粉酶添加至麵中,而於不對麵的彈力性或黏性等口感或風味造成影響的情況下,驚人地改良保存中的鬆開劣化。另外發現,藉由進一步併用水溶性半纖維素,而進一步改良麵的鬆開,從而完成了本發明。 The inventors of the present invention have conducted intensive studies in view of the above problems, and as a result, it has been found that by adding a lipase having a 1,3-position specificity and an amylase to the surface, the elastic or sticky surface is not opposite. In the case where the taste or the flavor is affected, the loosening deterioration during storage is surprisingly improved. Further, it has been found that the present invention is further improved by further improving the surface loosening by further using water-soluble hemicellulose.
即,本發明為:(1)一種麵類的製造方法,其特徵在於:將相對於1g原料粉而為1單元(unit)~20單元的具有1,3-位置特異性的脂肪酶及相對於1g原料粉而為0.1單元~5單元的澱粉酶添加至麵糰(dough)中;(2)如(1)所記載的麵類的製造方法,其中澱粉酶為α-澱粉酶;(3)如(1)或(2)所記載的麵類的製造方法,更含有水溶 性半纖維素;(4)如(3)所記載的麵類的製造方法,其中水溶性半纖維素來源於豆類;(5)如(4)所記載的麵類的製造方法,其中豆類為大豆或豌豆;(6)一種麵類,其是藉由如(1)至(5)中任一項所記載的製造方法而獲得;(7)一種麵類的鬆開改良方法,其特徵在於:將相對於1g原料粉而為1單元~20單元的具有1,3-位置特異性的脂肪酶及相對於1g原料粉而為0.1單元~5單元的澱粉酶添加至麵糰中;(8)一種麵用鬆開改良劑,其特徵在於:含有相對於1g原料粉而為1單元~20單元的具有1,3-位置特異性的脂肪酶及相對於1g原料粉而為0.1單元~5單元的澱粉酶。 That is, the present invention is: (1) A method for producing a noodle comprising a 1,3-position-specific lipase and a relative unit of 1 to 20 units per 1 g of the raw material powder. (1) The method for producing a noodle according to (1), wherein the amylase is an alpha-amylase; (3) The method for producing a noodle according to (1) or (2) further contains water-soluble (4) The method for producing a noodle according to (3), wherein the water-soluble hemicellulose is derived from a bean; (5) the method for producing a noodle according to (4), wherein the bean is (6) A noodle obtained by the production method according to any one of (1) to (5); (7) a method for improving the loosening of a noodle, characterized in that : adding 1 to 20 units of a lipase having a 1,3-position specificity to 1 g of the raw material powder, and adding 0.1 to 5 units of amylase to the dough with respect to 1 g of the raw material powder; (8) A surface release improving agent comprising: 1,3-position-specific lipase of 1 unit to 20 units with respect to 1 g of raw material powder, and 0.1 unit to 5 unit with respect to 1 g of raw material powder Amylase.
根據本發明,藉由將具有1,3-位置特異性的脂肪酶及澱粉酶添加至麵中,即便於保存後亦可改良麵類的鬆開性,同時亦有維持麵的彈力或黏性的效果。另外,藉由併用水溶性半纖維素而進一步改良鬆開性。 According to the present invention, by adding a lipase having a 1,3-position specificity and an amylase to the surface, the looseness of the noodle can be improved even after storage, and the elasticity or viscosity of the surface is maintained. Effect. Further, the release property is further improved by using water-soluble hemicellulose in combination.
以下,對本發明加以具體說明。 Hereinafter, the present invention will be specifically described.
(麵類) (Noodles)
所謂本發明的麵類,為乾麵、生麵、炒麵、熱風乾燥速食麵、冷凍乾燥速食麵等,具體可列舉:蕎麥、烏龍麵、扁麵條、中華麵、義大利麵(pasta)、通心麵(macaroni)、麵線、河粉(pho)、韓國冷麵、冬粉等。 The noodles of the present invention are dry noodles, noodles, fried noodles, hot air-dried instant noodles, freeze-dried instant noodles, and the like, and specific examples thereof include buckwheat, udon noodles, linguine noodles, Chinese noodles, pasta (pasta), and macaroni. Face (macaroni), noodle, pho, korea, winter powder, etc.
於該些麵類中,以家庭調理的麵為代表而包括以當即食用為目的之最終商品型的加工食品或食用時需要調理的半成品,且包含以常溫、冷藏、冷凍、冰溫(freezing temperature)等方法於市場上流通的食品。 Among these noodles, the family-conditioned side is represented by a finished product type processed food for immediate consumption or a semi-finished product to be conditioned when consumed, and includes room temperature, refrigeration, freezing, and freezing temperature. ) and other methods of food circulating in the market.
(脂肪酶) (Lipase)
本發明中使用的具有1,3-位置特異性的脂肪酶除了可應用來源於細菌、黴菌、酵母、絲狀菌、放線菌等微生物的脂肪酶以外,還可應用來源於胰臟、肝臟、乳、唾液等動物的脂肪酶以及來源於米糠、蓖麻、燕麥、小麥、菜籽、花生等植物的脂肪酶等。再者,所述脂肪酶除了經精製的脂肪酶以外,亦可為含有該些脂肪酶的粗酵素,例如來源於微生物的酵素的情況下亦包括:含有培養微生物而成的培養基成分的含脂肪酶的水溶液、以及將其乾燥而成的物品。 The lipase having a 1,3-position specificity used in the present invention may be applied to a pancreas or a liver, in addition to a lipase derived from a microorganism such as bacteria, mold, yeast, filamentous fungus or actinomycete. Lipases of animals such as milk and saliva, and lipases derived from plants such as rice bran, ramie, oats, wheat, rapeseed, and peanuts. Further, the lipase may be a crude enzyme containing the lipase in addition to the purified lipase, for example, an enzyme derived from a microorganism, and a fat containing a medium containing the cultured microorganism. An aqueous solution of the enzyme and an article obtained by drying the same.
1,3-位置特異性脂肪酶的來源較佳為來源於胰臟、肝臟、乳、唾液等動物的脂肪酶,微生物的情況下,較佳為麴菌(Aspergillus)屬、根毛黴(Rhizomucor)屬、疏棉狀嗜熱絲孢菌(Thermomyces)屬、酒麴菌(Rhizopus)屬、色桿菌 (Chromobacterium)屬、白黴菌(Mucor)屬、酵母菌(Saccharomycopsis)屬、鬚黴(Phycomyces)屬、產鹼桿菌(Alcaligenes)屬、假單胞菌(Pseudomonas)屬。 The source of the 1,3-position-specific lipase is preferably a lipase derived from an animal such as a pancreas, a liver, a milk or a saliva, and in the case of a microorganism, preferably a genus Aspergillus and Rhizomucor. Genus, Thermomyces, Rhizopus, and bacterium Chromobacterium, Mucor, Saccharomycopsis, Phycomyces, Alcaligenes, Pseudomonas.
對於本發明的脂肪酶而言,為了提高脂肪酶製劑的固體成分單位的脂質分解活性,例如較佳為使用通常的酵素精製法,或藉由使脂肪酶高分泌生產而提高脂肪酶的純度、精製度而成的脂肪酶。另外,脂肪酶也可使用經基因重組的脂肪酶。 In order to increase the lipolysis activity of the solid component unit of the lipase preparation, the lipase of the present invention preferably has a normal enzyme purification method or a lipase high secretion production to increase the purity of the lipase. A lipase that is made by a fine system. In addition, lipases may also use genetically recombinant lipases.
脂肪酶中,是否具有1,3-位置特異性例如可藉由「定量地表現脂肪酶的位置特異性的指數」(醱酵工學會雜誌,第5號,405-421(1988年))中記載的試驗方法來評價。若提及市售的酵素,則可例示:脂肪酶A「天野(Amano)」6、脂肪酶G「天野(Amano)」50、脂肪酶DF「天野(Amano)」15(天野酶製品(Amano Enzyme)),脂肪酶A-10D(長瀨化成(Nagase Chemtex)),貝克塞姆(Bakezyme)L80000B(DSM)等。另一方面,不具有1,3-位置特異性的脂肪酶例如可列舉:脂肪酶AY「天野(Amano)」30D-K(天野酶製品(Amano Enzyme)),脂肪酶MY、脂肪酶OF(名糖產業)等。 Whether or not the lipase has a 1,3-position specificity can be, for example, "an index that quantitatively expresses the positional specificity of a lipase" (Journal of the Institute of Enzyme Engineering, No. 5, 405-421 (1988)) The test methods described are evaluated. When a commercially available enzyme is mentioned, it can be exemplified by lipase A "Amano" 6, lipase G "Amano" 50, lipase DF "Amano" 15 (Amano enzyme product (Amano) Enzyme)), lipase A-10D (Nagase Chemtex), Bakezyme L80000B (DSM), and the like. On the other hand, examples of the lipase having no 1,3-position specificity include lipase AY "Amano" 30D-K (Amano Enzyme), lipase MY, lipase OF ( Famous sugar industry) and so on.
本發明的具有1,3-位置特異性的脂肪酶較佳為相對於麵所使用的原料粉1g而以1單元~20單元的範圍調配,進而佳為以2單元~15單元的範圍調配。若具有1,3-位置特異性的脂肪酶的含量過少,則麵的鬆開性變得不充分,若具有1,3-位置特異性的脂肪酶的含量過多,則有時產生源自脂肪酸的不良風味。 The lipase having a 1,3-position specificity of the present invention is preferably formulated in a range of from 1 unit to 20 units, relative to 1 g of the raw material powder used in the surface, and more preferably in a range of from 2 to 15 units. When the content of the lipase having a 1,3-position specificity is too small, the surface looseness is insufficient, and if the content of the lipase having a 1,3-position specificity is too large, the fatty acid may be derived from the fatty acid. Bad flavor.
再者,本發明中所謂的具有1,3-位置特異性的脂肪酶的1單元,是指藉由JIS0601<sup>-1995</sup>「工業用脂肪酶的活性測定方法」所示的方法而測定。即,所謂1單元,表示於預定條件下於1分鐘內使1μmol的脂肪酸自基質(橄欖油)中游離的酵素量。 In addition, the one unit of the lipase having a 1,3-position specificity in the present invention is represented by JIS0601<sup>-1995</sup> "Method for measuring the activity of industrial lipase". Determined by the method. That is, the one unit means the amount of the enzyme which releases 1 μmol of the fatty acid from the matrix (olive oil) in 1 minute under predetermined conditions.
作為脂肪酶有助於麵的鬆開的理由,可列舉:於麵的麵糰中將小麥所含的油脂分解,生成單甘油酯或二甘油酯。推測所生成的單甘油酯或二甘油酯作用於麵的麵糰的澱粉等,改善鬆開性。推測具有1,3-位置特異性的脂肪酶中,2位的脂肪酸經切斷殘留,故於單甘油酯的狀態下大多反應停止,但隨機作用的脂肪酶中,所有位置的脂肪酸被切斷,故不易鬆開,而容易出現源自脂肪酸的不良風味 The reason why the lipase contributes to the loosening of the surface is that the fat contained in the wheat is decomposed in the dough of the dough to form a monoglyceride or a diglyceride. It is presumed that the produced monoglyceride or diglyceride acts on the starch of the dough on the surface to improve the looseness. It is presumed that among the lipases having a 1,3-position specificity, the fatty acids at the 2-position are cleaved, so that most of the reaction stops in the state of the monoglyceride, but in the lipase which is randomly applied, the fatty acids at all positions are cut off. Therefore, it is not easy to loosen, and it is prone to undesirable flavor derived from fatty acids.
(澱粉酶) (amylase)
本發明中使用的澱粉酶可例示:來源於牛.豬的胰臟等動物的澱粉酶,來源於麥芽、大麥、小麥、番薯、大豆等植物的澱粉酶,來源於細菌、黴菌、酵母、絲狀菌、放線菌等微生物的澱粉酶等。再者,所述澱粉酶除了經精製的澱粉酶以外,亦可為含有該些澱粉酶的粗酵素,例如來源於微生物的酵素的情況下包括:含有培養微生物所得的培養基成分的含澱粉酶的水溶液、以及將其乾燥所得的物品。來源於微生物的情況下,可列舉來源於桿菌(Bacillus)屬、麴菌(Aspergillus)屬的澱粉酶,可例示α-澱粉酶、β-澱粉酶、葡糖澱粉酶等,其中最佳為α-澱粉酶。 The amylase used in the present invention can be exemplified by: derived from cattle. The amylase of animals such as pig pancreas is derived from amylase of plants such as malt, barley, wheat, sweet potato, soybean, etc., and is derived from amylases of microorganisms such as bacteria, mold, yeast, filamentous fungi, and actinomycetes. Furthermore, the amylase may be a crude enzyme containing the amylase in addition to the purified amylase, for example, an enzyme derived from a microorganism, including an amylase containing a medium component obtained by culturing the microorganism. An aqueous solution, and an article obtained by drying it. In the case of a microorganism, an amylase derived from the genus Bacillus or Aspergillus may be mentioned, and α-amylase, β-amylase, glucoamylase, etc. may be exemplified, and α is preferably α. - Amylase.
該些澱粉酶可單獨使用或混合使用兩種以上。 These amylases may be used alone or in combination of two or more.
澱粉酶較佳為相對於麵所使用的原料粉1g而以0.1單元/g~5單元/g的範圍調配,進而佳為以0.2單元/g~3單元/g的範圍調配。若澱粉酶的含量過少則麵的鬆開性變得不充分,若澱粉酶的含量過多則有時麵變得過於柔軟。 The amylase is preferably blended in a range of 0.1 unit/g to 5 units/g with respect to 1 g of the raw material powder used in the surface, and more preferably in a range of 0.2 unit/g to 3 units/g. When the content of the amylase is too small, the looseness of the surface becomes insufficient, and if the content of the amylase is too large, the surface may become too soft.
再者,本發明中所謂的澱粉酶的1單元,是指藉由現有添加物自主標準中記載的「α-澱粉酶活性測定法」所示的方法而測定。即,所謂1單元,表示以馬鈴薯澱粉作為基質而於預定條件下進行試驗時,於1分鐘內使澱粉的由碘所致的藍色減少10%的酵素量。 In addition, the one unit of the amylase in the present invention is measured by the method described in the "α-amylase activity measurement method" described in the existing additive autonomy standard. In other words, the "unit" indicates the amount of enzyme which reduces the blue color of iodine due to iodine by 10% in one minute when the test is carried out under predetermined conditions using potato starch as a substrate.
本發明中,原料粉是用於麵的麵糰,大多使用小麥粉,亦可含有大麥、黑麥(rye)、大豆、其他豆類、蕎麥、米、玉米等穀粉。另外,亦可含有澱粉、加工澱粉、糖類、水溶性食物纖維、不溶性食物纖維、豆乳粉末、大豆蛋白質、乳蛋白質、乳清、蛋白、蛋黃、膠原蛋白等蛋白質及蛋白質分解物以及肽。本發明中,將該些物質作為原料粉。 In the present invention, the raw material powder is a dough for the surface, and most of the wheat flour is used, and may also contain cereal flour such as barley, rye, soybean, other beans, buckwheat, rice, corn, and the like. In addition, it may also contain protein, protein degradation products and peptides such as starch, processed starch, sugar, water-soluble dietary fiber, insoluble dietary fiber, soy milk powder, soy protein, milk protein, whey, protein, egg yolk, collagen, and the like. In the present invention, these materials are used as raw material powders.
(水溶性半纖維素) (water soluble hemicellulose)
水溶性半纖維素是將油籽(oilseed)(棕櫚、椰子、玉米、棉籽等)或穀類(米、小麥等)或豆類(大豆、小豆、豌豆等)作為原料而獲得,就溶解性或工業性的方面而言,本發明中較佳為來源於豆類,更佳為大豆或豌豆,進而佳為大豆,其中較佳為來源於籽葉。 Water-soluble hemicellulose is obtained by using oilseed (palm, coconut, corn, cottonseed, etc.) or cereals (rice, wheat, etc.) or beans (soybeans, adzuki beans, peas, etc.) as raw materials, in terms of solubility or industry. In terms of properties, in the present invention, it is preferred to be derived from beans, more preferably soybeans or peas, and more preferably soybeans, and preferably derived from seed leaves.
水溶性半纖維素可藉由以下方法而獲得:自含有半纖維素的原料中進行水萃取或熱水萃取的方法,於酸、鹼條件下進行加熱萃取的方法,藉由酵素分解進行萃取的方法等。 The water-soluble hemicellulose can be obtained by a method of performing water extraction or hot water extraction from a hemicellulose-containing raw material, heating and extracting under acid and alkali conditions, and extracting by enzyme decomposition. Method, etc.
若示出使用大豆作為原料的情形的水溶性半纖維素的製造法的較佳一例,則如以下所述。將該些原料於酸性至鹼性的條件下、較佳為各蛋白質的等電點附近的pH值下,較佳為於80℃以上且130℃以下、更佳為超過100℃且130℃以下進行加熱分解,藉由離心分離等將水溶性組分分離後,直接乾燥,或例如進行活性炭處理或樹脂吸附處理或乙醇沈澱處理,將疏水性物質或低分子物質去除並乾燥,藉此可獲得水溶性半纖維素。 A preferred example of the method for producing water-soluble hemicellulose in the case of using soybean as a raw material is as follows. Preferably, the raw materials are at a pH of from about 80 ° C to 130 ° C, more preferably more than 100 ° C and less than 130 ° C under acidic to alkaline conditions, preferably at a pH near the isoelectric point of each protein. Performing thermal decomposition, separating the water-soluble component by centrifugation or the like, directly drying, or performing, for example, activated carbon treatment or resin adsorption treatment or ethanol precipitation treatment, removing or drying the hydrophobic substance or the low molecular substance, thereby obtaining Water soluble hemicellulose.
本發明的水溶性半纖維素的較佳添加量並無特別限制,相對於麵所使用的粉而為0.01重量%~5重量%,進而佳為0.05重量%~3重量%。 The amount of the water-soluble hemicellulose to be added in the invention is not particularly limited, and is 0.01% by weight to 5% by weight, and more preferably 0.05% by weight to 3% by weight based on the powder used for the surface.
(蛋白質) (protein)
本發明中所用的蛋白質較佳為使用以大豆、豌豆、綠豆等豆類,小麥、蛋白作為原料的熱凝固性蛋白質,更佳為大豆、豌豆、綠豆等豆類的蛋白質。另外,就可抑制成本的方面、可對口感進一步賦予黏性及彈力的方面而言,進而佳為大豆蛋白質。大豆蛋白質可例示:分離大豆蛋白質、鹼土金屬結合分離大豆蛋白質、濃縮大豆蛋白質、全脂豆乳、全脂豆乳粉末、脫脂豆乳、脫脂豆乳粉末、脫脂豆乳或脫脂豆乳粉末的鹼土金屬結合物等。該些蛋白質可併用一種以上而使用。 The protein to be used in the present invention is preferably a thermocoagulable protein using soybeans, peas, mung beans or the like, wheat or protein as a raw material, and more preferably a bean such as soybean, pea or mung bean. In addition, it is possible to suppress the cost, and it is possible to further impart viscosity and elasticity to the mouthfeel, and further preferably soybean protein. The soybean protein can be exemplified by separation of soy protein, alkaline earth metal combined with soy protein, concentrated soy protein, whole fat soy milk, whole fat soy milk powder, defatted soy milk, defatted soy milk powder, defatted soy milk or alkaline earth metal conjugate of defatted soy milk powder. These proteins may be used in combination of one or more.
蛋白質的製造法只要為食品製造中的通常方法,則並無特別限制,經由加水萃取、等電點沈澱、膜分離、水洗、精製、脫糖等步驟而製造。視需要亦可使用藉由酸、鹼、酵素等使該些蛋白質分解而成的物質。 The method for producing the protein is not particularly limited as long as it is a usual method in food production, and is produced by steps such as water extraction, isoelectric precipitation, membrane separation, water washing, purification, and sugar removal. A substance obtained by decomposing the proteins by an acid, a base, or an enzyme may be used as needed.
本發明的麵類的麵糰中,必須調配具有1,3-位置特異性的脂肪酶及澱粉酶,除了水溶性半纖維素以外,可適當根據調整麵質等目的而併用其他添加劑。關於其他添加劑,若併用選自以下物質的組群中的一種以上的乳化劑,則麵的鬆開性、麵質得到改良:葡萄糖、果糖等單糖類,蔗糖、麥芽糖、乳糖、棉籽糖、麥芽三糖、海藻糖(trehalose)、水蘇糖(stachyose)、麥芽四糖等少糖類,及糖醇、糊精、海蘿、瓊脂(agar)、卡拉膠(carrageenan)、帚叉藻膠、羅望子(tamarind)種子多糖類,刺雲豆膠、刺梧桐膠、黃蓍膠(Tragacanth gum)、果膠、三仙膠、海藻酸鈉、瓜爾膠、印度樹膠、刺槐豆膠、普魯蘭多糖、結冷膠(Gellan gum)、阿拉伯樹膠、卡德蘭膠、結晶纖維素、甲基纖維素、羥基丙基甲基纖維素、玻脲酸、環糊精、殼聚糖、羧基甲基纖維素(CMC)、海藻酸丙二醇酯、加工澱粉等各種澱粉類等多糖類或該些多糖類的水解物,乳、大豆、蛋、小麥等蛋白質,明膠、可溶性膠原蛋白、鈣強化劑等鹽類,乙酸、檸檬酸、乳酸、己二酸、葡萄糖酸等酸味料,單甘油脂肪酸酯、有機酸單甘油脂肪酸酯、卵磷脂及硬脂醯基乳酸鹽。除此以外,亦可併用蔗糖脂肪酸酯、聚甘油脂肪酸酯、山梨醇酐脂肪酸酯、丙二醇脂肪酸酯、聚山梨醇酯等乳化劑。 In the dough of the noodle of the present invention, it is necessary to mix a lipase having a 1,3-position specificity and an amylase, and in addition to the water-soluble hemicellulose, other additives may be used in combination according to the purpose of adjusting the surface quality. When one or more emulsifiers selected from the group consisting of the following substances are used together, the surface release property and the surface quality are improved: monosaccharides such as glucose and fructose, sucrose, maltose, lactose, raffinose, and wheat. Less sugars such as buds trisaccharide, trehalose, stachyose, maltotetraose, and sugar alcohol, dextrin, dill, agar, carrageenan, and tartar gum , tamarind seed polysaccharides, thorn bean gum, karaya gum, tragacanth gum, pectin, sambag, sodium alginate, guar gum, Indian gum, locust bean gum, pu Lulan polysaccharide, gellan gum, gum arabic, gelantan gum, crystalline cellulose, methyl cellulose, hydroxypropyl methylcellulose, hyaluronic acid, cyclodextrin, chitosan, carboxyl Polysaccharides such as methyl cellulose (CMC), propylene glycol alginate, processed starch, etc., or hydrolysates of these polysaccharides, proteins such as milk, soybean, egg, wheat, gelatin, soluble collagen, calcium fortifier Equal salt, acetic acid, citric acid, lactic acid, adipic acid Acidulants gluconic acid, monoglycerol fatty acid ester, organic acid monoglycerin fatty acid ester, lecithin, stearyl and acyl lactates. In addition to this, an emulsifier such as sucrose fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester or polysorbate may be used in combination.
將具有1,3-位置特異性的脂肪酶及澱粉酶、較佳為進而水溶性半纖維素添加至麵類中的方法並無特別限定,作為混入至調理麵、速食麵或義大利麵的麵的麵糰中的方法,可例示:(1)於小麥粉等中進行粉混合而添加的方法,(2)與鹽或鹼水、調味料一起溶解於混練水中而添加的方法。該些方法是根據製造線、銷售形態等各食品特性而選擇。 A method of adding a lipase having a 1,3-position specificity and an amylase, preferably a water-soluble hemicellulose, to a noodle is not particularly limited, and is a surface mixed into a conditioning surface, an instant noodles or a pasta surface. The method of the dough may be, for example, a method in which powder is mixed and added to wheat flour or the like, and (2) a method in which it is dissolved in kneading water together with a salt or an alkali water or a seasoning. These methods are selected in accordance with the characteristics of each food product such as a manufacturing line and a sales form.
為了使具有1,3-位置特異性的脂肪酶及澱粉酶、較佳為進而使水溶性半纖維素於麵的麵糰中有效率地發揮作用,可於調配麵的麵糰時與本鬆開改良劑同時添加油脂。所添加的油脂並無特別限制,可使用來源於動植物的油脂。可例示豬油、牛油、乳脂、蛋黃、大豆油、菜籽油、可可脂、米糠油、向日葵油、玉米油、棉籽油、棕櫚油、椰子油、芝麻油、橄欖油、蘇子油(perilla)等。或者亦可使用該些油脂硬化、分別經酯交換而成的油脂。該些油脂中,也將一種或兩種以上的油脂調配而使用。 In order to efficiently act on the 1,3-position-specific lipase and amylase, and preferably the water-soluble hemicellulose in the dough, it is possible to improve the dough and the loosening of the dough. Add grease at the same time. The oil to be added is not particularly limited, and oils derived from animals and plants can be used. Examples include lard, butter, cream, egg yolk, soybean oil, rapeseed oil, cocoa butter, rice bran oil, sunflower oil, corn oil, cottonseed oil, palm oil, coconut oil, sesame oil, olive oil, and perilla oil (perilla). )Wait. Alternatively, it is also possible to use the fats and oils which are hardened by the fats and oils, and are each transesterified. Among these fats and oils, one or two or more kinds of fats and oils are also used in combination.
藉由將具有1,3-位置特異性的脂肪酶及澱粉酶、較佳為進而將水溶性半纖維素添加至麵類中的方法所得的麵類具有良好的鬆開性。因此,藉由本發明所得的麵類非常容易食用,且美味。另外,例如調理麵的情況下,即便製備後冷藏保存1天~2天後亦維持其鬆開性,明顯抑制麵彼此的黏著,亦維持麵的彈力或黏性。另外,藉由賦予良好的鬆開性,於麵類的製造中有容易混合、不易破碎等優異的加工適性。 The noodle obtained by the method of adding a lipase having a 1,3-position specificity and an amylase, preferably a water-soluble hemicellulose to a noodle, has good release property. Therefore, the noodles obtained by the present invention are very easy to eat and delicious. Further, for example, in the case of a conditioning surface, the detachability is maintained even after being stored and stored for one to two days, and the adhesion of the surfaces is remarkably suppressed, and the elasticity or viscosity of the surface is maintained. Further, by imparting good release property, it is excellent in workability in the production of noodle, which is easy to mix and is not easily broken.
(麵用鬆開改良劑) (face loosening modifier)
本發明的麵用鬆開改良劑的特徵在於:含有相對於1g原料粉而為1單元~20單元的具有1,3-位置特異性的脂肪酶及相對於1g原料粉而為0.1單元~5單元的澱粉酶。進而,若含有水溶性半纖維素,則麵的鬆開性改良的功能提高,故較佳。 The surface release improving agent of the present invention is characterized in that it contains 1 to 20 units of a lipase having a 1,3-position specificity with respect to 1 g of the raw material powder, and 0.1 to 5 with respect to 1 g of the raw material powder. Unit of amylase. Further, when water-soluble hemicellulose is contained, the function of improving the surface release property is improved, which is preferable.
本鬆開改良劑於麵類中的添加量亦視麵的種類或鬆開改良劑的配方而不同,可例示:相對於小麥粉等的原料粉的重量,較佳為0.001重量%~5重量%,更佳為0.002重量%~3重量%。 The amount of the sizing agent to be added to the noodle is also different depending on the type of the surface or the formulation of the sizing agent, and it is preferably 0.001% by weight to 5 parts by weight based on the weight of the raw material powder such as wheat flour. More preferably, it is 0.002% by weight to 3% by weight.
[實施例] [Examples]
以下記載實施例。 The examples are described below.
再者,例中的份及%是指重量基準。 Furthermore, the parts and % in the examples refer to the weight basis.
(實施例1) (Example 1)
於600g小麥粉、390g木薯澱粉及10g小麥麩質的混合物中,添加將20g食鹽、0.1g的作為具有1,3-位置特異性的脂肪酶的脂肪酶A「天野(Amano)」6(天野酶製品(Amano Enzyme):60,000單元/g)、作為澱粉酶的斯密塞姆(Sumizyme)AS(新日本化學工業(股)製造:1500單元/g)溶解於450g水中所得的混練水進行混練,製作麵的麵糰。將麵糰壓延,以#20的切齒切出而獲得中華麵作為實施例1。將中華麵煮80秒鐘後水洗,以100g為單位細分至龜甲容器中,於4℃下保存2天。對該些中華麵評價鬆開性、麵質(彈力性、硬度)。 In a mixture of 600 g of wheat flour, 390 g of tapioca starch, and 10 g of wheat gluten, 20 g of salt and 0.1 g of lipase A as a 1,3-position-specific lipase were added, "Amano" 6 (Tano) Enzyme preparation (Amano Enzyme): 60,000 units/g), as a amylase, Sumizyme AS (manufactured by Nippon Chemical Industry Co., Ltd.: 1500 units/g), kneaded water obtained by dissolving in 450 g of water for kneading , making dough for the noodles. The dough was calendered, and cut into teeth of #20 to obtain a Chinese noodle as Example 1. The Chinese noodles were boiled for 80 seconds, washed with water, and subdivided into a tortoise shell container in units of 100 g, and stored at 4 ° C for 2 days. The looseness and the texture (elasticity, hardness) of the Chinese noodles were evaluated.
(實施例2) (Example 2)
於實施例1中記載的方法中,使用脂肪酶DF「天野(Amano)」 15(天野酶製品(Amano Enzyme)(股)製造:150,000單元/g)作為具有1,3-位置特異性的脂肪酶,且使用比奧塞姆(Biozyme)A(天野酶製品(Amano Enzyme)(股)製造:7500單元/g)作為澱粉酶,除此以外,利用相同的方法製作中華麵。 In the method described in Example 1, lipase DF "Amano" was used. 15 (Amano Enzyme (manufactured by Amano Enzyme): 150,000 units/g) as a lipase having a 1,3-position specificity, and using Biozyme A (Amano Enzyme) (manufacturing): 7500 units/g) As the amylase, the Chinese noodles were produced by the same method.
(比較例1) (Comparative Example 1)
於實施例1中記載的方法中,不使用脂肪酶,除此以外,利用相同的方法製備中華麵。 In the method described in Example 1, except that lipase was not used, the Chinese noodle was prepared by the same method.
(比較例2) (Comparative Example 2)
於實施例1中記載的方法中,使用0.1g的脂肪酶A「天野(Amano)」6(天野酶製品(Amano Enzyme)(股)製造:60,000單元/g)作為具有1,3-位置特異性的脂肪酶,除此以外,利用相同的方法製備中華麵。 In the method described in Example 1, 0.1 g of lipase A "Amano" 6 (manufactured by Amano Enzyme (manufactured by Amano Enzyme): 60,000 units/g) was used as having a 1,3-position specificity. In addition to the sexual lipase, the Chinese noodle was prepared by the same method.
(比較例3) (Comparative Example 3)
於實施例1中記載的方法中,使用脂肪酶DF「天野(Amano)」15(天野酶製品(Amano Enzyme)(股)製造:150,000單元/g)作為具有1,3-位置特異性的脂肪酶,除此以外,利用相同的方法製備中華麵。 In the method described in Example 1, lipase DF "Amano" 15 (manufactured by Amano Enzyme (manufactured by Amano Enzyme): 150,000 units/g) was used as the fat having 1,3-position specificity. In addition to the enzyme, the Chinese noodles were prepared by the same method.
(比較例4) (Comparative Example 4)
於實施例1中記載的方法中,使用比奧塞姆(Biozyme)A(天野酶製品(Amano Enzyme)(股)製造)作為澱粉酶,除此以外,利用相同的方法製備中華麵。 In the method described in Example 1, except that Biozyme A (manufactured by Amano Enzyme Co., Ltd.) was used as the amylase, the Chinese noodles were prepared by the same method.
(比較例5) (Comparative Example 5)
於實施例1中記載的方法中,僅使用作為蛋白酶的斯密塞姆(Sumizyme)LPL(新日本化學工業(股)製造:500,000單元/g)作為酵素,除此以外,利用相同的方法製備中華麵。 In the method described in the first embodiment, only the Sumizyme LPL (manufactured by Shin-Nippon Chemical Industry Co., Ltd.: 500,000 units/g) as a protease was used as an enzyme, and the same method was used. Chinese noodles.
(比較例6) (Comparative Example 6)
於實施例1中記載的方法中,使用不具有1,3-位置特異性的脂肪酶AY「天野(Amano)」30(天野酶製品(Amano Enzyme)(股)製造:30,000單元/g)作為脂肪酶,且使用斯密塞姆(Sumizyme)AS作為澱粉酶,除此以外,利用相同的方法製備中華麵。 In the method described in Example 1, a lipase AY "Amano" 30 (manufactured by Amano Enzyme: 30,000 units/g) having no 1,3-position specificity was used. In addition to the lipase, and using Sumizyme AS as the amylase, the Chinese noodle was prepared by the same method.
(比較例7) (Comparative Example 7)
於實施例1中記載的方法中,使用不具有1,3-位置特異性的脂肪酶MY(名糖產業(股)製造:32,000單元/g)作為脂肪酶,且使用斯密塞姆(Sumizyme)AS作為澱粉酶,除此以外,利用相同的方法製備中華麵。 In the method described in Example 1, lipase MY (manufactured by the famous sugar industry: 32,000 units/g) having no 1,3-position specificity was used as a lipase, and Sumizyme was used. As the amylase, AS was prepared by the same method.
○麵的評價方法 ○face evaluation method
於經2天保存的麵中添加麵湯汁,目測觀察鬆開的狀態。將鬆開性非常良好視為5分、鬆開性非常差視為1分而以5等級進行評價,將6名官能審查員的評價的平均值作為評價分。若平均值為3.0點以上則視為合格。 The noodle soup was added to the surface which was preserved for 2 days, and the state of loosening was visually observed. The release property was considered to be very good as 5 points, the release property was very poor as 1 point, and the evaluation was performed on the 5th grade, and the average value of the evaluations of the six sensory examiners was used as the evaluation score. If the average value is 3.0 points or more, it is considered acceptable.
關於麵的口感,由6名熟練的官能審查員來評價彈力及黏性。將彈力、黏性均非常良好視為5分,將非常差視為1分而以5等級進行評價,算出平均分。若平均值為3.0點以上則視為合格。 Regarding the taste of the noodles, six skilled functional examiners evaluated the elasticity and viscosity. The elastic strength and the viscousness were regarded as very good as 5 points, and the very poor was regarded as 1 point and evaluated by 5 levels, and the average score was calculated. If the average value is 3.0 points or more, it is considered acceptable.
關於麵的風味,將感覺到異味的情況視為×,其他情況視為○,將○的情況視為合格。 Regarding the flavor of the noodle, the case where the odor was felt was regarded as ×, the other case was regarded as ○, and the case where ○ was regarded as the pass.
在綜合評價中,將麵的鬆開性、口感、風味均為合格的情況評價為○而視為合格。於麵的鬆開性、口感、風味的任一個不合格的情形時,綜合評價是評價為×而視為不合格。 In the comprehensive evaluation, the case where the looseness, the mouthfeel, and the flavor of the surface were all qualified was evaluated as ○ and was regarded as acceptable. In the case where any of the looseness, texture, and flavor of the surface is unacceptable, the overall evaluation is evaluated as × and is considered as unqualified.
(脂肪酶的活性測定方法) (Method for measuring the activity of lipase)
關於脂肪酶的水解活性的測定,依照JIS0601<sup>-1995</sup>「工業用脂肪酶的活性測定方法」進行測定。 The measurement of the hydrolysis activity of the lipase was carried out in accordance with JIS0601 <sup>-1995</sup> "Method for measuring the activity of industrial lipase".
(澱粉酶的活性測定方法) (Method for measuring the activity of amylase)
關於澱粉酶的水解活性的測定,依照現有添加物自主標準中記載的「α-澱粉酶活性測定法」進行測定。 The measurement of the hydrolysis activity of the amylase was carried out in accordance with the "α-amylase activity measurement method" described in the existing additive autonomy standard.
將實施例1~實施例2、比較例1~比較例7的評價結果示於表1中。 The evaluation results of Examples 1 to 2 and Comparative Examples 1 to 7 are shown in Table 1.
於併用具有1,3-位置特異性的脂肪酶與澱粉酶的實施例1、實施例2中,可獲得非常良好的鬆開及口感,相較於將具有1,3-位置特異性的脂肪酶或澱粉酶分別單獨添加的情況,鬆開性變良好。另一方面,於使用不具有1.3-位置特異性的脂肪酶的情形時,中華麵(比較例6~比較例7)的鬆開性不充分,且麵中感覺到刺激性的異味。 In Example 1, Example 2, in which a lipase having a 1,3-position specificity and an amylase were used in combination, a very good release and mouthfeel was obtained, compared to a fat having a 1,3-position specificity. When the enzyme or the amylase is separately added, the release property becomes good. On the other hand, when a lipase having no 1.3-position specificity was used, the looseness of the Chinese noodles (Comparative Examples 6 to 7) was insufficient, and a pungent odor was felt in the surface.
(脂肪酶、澱粉酶的添加量) (addition amount of lipase and amylase)
(實施例3~實施例12、比較例8~比較例11) (Example 3 to Example 12, Comparative Example 8 to Comparative Example 11)
於實施例1中記載的方法中,如表2所示般添加脂肪酶「天野(Amano)」6作為具有1,3-位置特異性的脂肪酶,且添加比奧塞姆(Biozyme)A作為澱粉酶,將所得的中華麵作為實施例3~實施例12、比較例8~比較例11。將評價結果示於表2中。 In the method described in Example 1, as shown in Table 2, the lipase "Amano" 6 was added as a lipase having a 1,3-position specificity, and Biozyme A was added as the Amylase, the obtained Chinese noodles were used as Examples 3 to 12 and Comparative Examples 8 to 11. The evaluation results are shown in Table 2.
具有1,3-位置特異性的脂肪酶為1單元/g~20單元/g的範圍及澱粉酶為0.1單元/g~5單元/g的範圍的添加量下,顯示出麵的良好的鬆開性、口感、風味。具有1,3-位置特異性的脂肪酶或澱粉酶的添加在這些範圍外的比較例8~比較例11中,鬆開性變差,或即便鬆開性良好但口感差。 The lipase having a 1,3-position specificity is in the range of 1 unit/g to 20 units/g and the amylase is in the range of 0.1 unit/g to 5 unit/g, showing a good pine surface. Open, taste, flavor. In the comparative examples 8 to 11 in which the addition of the lipase or the amylase having the 1,3-position specificity was out of the above range, the release property was deteriorated, or the mouthfeel was poor even if the release property was good.
(實施例13)水溶性半纖維素的併用 (Example 13) Combination of water-soluble hemicellulose
在實施例1中,與具有1,3-位置特異性的脂肪酶、澱粉酶一起添加1g的作為水溶性半纖維素的水溶性大豆多糖類(商品名:大豆纖維(Soyafibe)-S,不二製油股份有限公司製造),除此以外,與實施例1同樣地製備中華麵,作為實施例13。 In Example 1, 1 g of a water-soluble soybean polysaccharide as a water-soluble hemicellulose (trade name: Soyafibe-S) was added together with a lipase having an 1,3-position specificity and an amylase. A Chinese noodle was prepared in the same manner as in Example 1 except that the production was carried out in the same manner as in Example 1.
(實施例14)水溶性豌豆多糖類的併用 (Example 14) Combination of water-soluble pea polysaccharides
於實施例1中,與具有1,3-位置特異性的脂肪酶、澱粉酶一起而添加1g的作為水溶性半纖維素的水溶性豌豆多糖類,除此以外,與實施例1同樣地製備中華麵,作為實施例14。 In the same manner as in Example 1, except that 1 g of a water-soluble pea polysaccharide which is water-soluble hemicellulose was added together with a lipase having a 1,3-position specificity and an amylase, the same procedure as in Example 1 was carried out. Chinese noodles, as Example 14.
再者,水溶性豌豆多糖類是利用以下方法來製備。 Further, the water-soluble pea polysaccharides were prepared by the following method.
將50kg的豌豆種子脫皮後,添加5倍量的水並浸漬24小時。利用均質機(5,000rpm、30分鐘)將種子粉碎,萃取蛋白質及澱粉。使用離心過濾機以1,500×g、20分鐘的條件將分散於水中的蛋白質或澱粉等成分去除,回收纖維質。進而,於纖維質中添加5倍量的水並利用均質機(3,000rpm、30分鐘)進行攪拌,藉由離心過濾(1,500×g、20分鐘)回收纖維質。將該操作重複2次,進行冷凍乾燥而獲得10kg的豌豆纖維。將80份的豌豆纖維分散在920份的水中,使用鹽酸將pH值調整為5後,於120℃下加熱90分鐘而萃取水溶性豌豆多糖類。離心分離(5000rpm、30分鐘)後,於上清液中以成為60重量%的方式添加乙醇而使水溶性豌豆多糖類沈澱,以90重量%的含水乙醇進行精製,將所得的沈澱風乾而獲得水溶性豌豆多糖類。 After peeling 50 kg of pea seeds, 5 times the amount of water was added and immersed for 24 hours. The seeds were pulverized by a homogenizer (5,000 rpm, 30 minutes) to extract proteins and starch. The components such as protein or starch dispersed in water were removed using a centrifugal filter under conditions of 1,500 × g for 20 minutes to recover the fiber. Further, five times the amount of water was added to the fiber, and the mixture was stirred by a homogenizer (3,000 rpm, 30 minutes), and the fiber was recovered by centrifugal filtration (1,500 × g, 20 minutes). This operation was repeated twice, and lyophilized to obtain 10 kg of pea fiber. 80 parts of pea fiber was dispersed in 920 parts of water, and the pH was adjusted to 5 with hydrochloric acid, and then heated at 120 ° C for 90 minutes to extract water-soluble pea polysaccharides. After centrifugation (5000 rpm, 30 minutes), ethanol was added to the supernatant so as to be 60% by weight to precipitate a water-soluble pea polysaccharide, and it was purified by 90% by weight of aqueous ethanol, and the obtained precipitate was air-dried. Water soluble pea polysaccharides.
(實施例15)大豆蛋白的併用 (Example 15) Combination of soy protein
於實施例1中,與具有1,3-位置特異性的脂肪酶一起而添加1g的作為大豆蛋白的新不二普羅(New Fuji Pro)SEH(不二製油股份有限公司製造),除此以外,與實施例1同樣地製備中華麵,作為實施例15。 In the first embodiment, 1 g of New Fuji Pro SEH (manufactured by Fuji Oil Co., Ltd.) as a soybean protein was added together with a lipase having a 1,3-position specificity, and the like. A Chinese noodle was prepared in the same manner as in Example 1 as Example 15.
將實施例13~實施例15的評價結果示於表3中。 The evaluation results of Examples 13 to 15 are shown in Table 3.
若除了具有1,3-位置特異性的脂肪酶、澱粉酶以外進一步併用水溶性半纖維素,則鬆開性進一步提高。若於具有1,3-位置特異性的脂肪酶中併用大豆蛋白,則於口感方面賦予有彈力。 When the water-soluble hemicellulose is further used in addition to the lipase or amylase having a 1,3-position specificity, the release property is further improved. When soy protein is used in combination with a lipase having a 1,3-position specificity, elasticity is imparted to the mouthfeel.
○烏龍麵的評價(實施例16) ○ Evaluation of Udon Noodles (Example 16)
於800g小麥粉及200g木薯澱粉的混合物中,加入添加30g食鹽、實施例13的配方中具有1,3-位置特異性的脂肪酶、澱粉酶及水溶性半纖維素並溶解於360g水中所得的混練水進行混練,製作麵的麵糰。將麵糰壓延,以#10的切齒切出而製成烏龍麵。將烏龍麵煮10分鐘後進行水洗,以100g為單位細分至龜甲容器中,於4℃下保存2天。 In a mixture of 800 g of wheat flour and 200 g of tapioca starch, a lipase, an amylase and a water-soluble hemicellulose having a 1,3-position specificity in a formulation of Example 13 added in 30 g of salt were dissolved and dissolved in 360 g of water. Mix the water and mix it to make the dough. The dough was calendered and cut out with a #10 cut tooth to make a udon. The oolong noodles were boiled for 10 minutes, washed with water, and subdivided into a tortoise shell container in units of 100 g, and stored at 4 ° C for 2 days.
實施例16的烏龍麵於保存2天後亦維持良好的鬆開性,且具有彈力及黏性的良好口感。 The oolong noodles of Example 16 also maintained good release properties after 2 days of storage, and had a good mouthfeel with elasticity and viscosity.
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| CN112617106B (en) * | 2020-12-18 | 2023-06-20 | 湖州草本连生物科技有限公司 | Selenium Enriched Golden Wheat Noodles |
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