TWI471161B - A manufacturing method of antrodia cinnamomea - Google Patents
A manufacturing method of antrodia cinnamomea Download PDFInfo
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- 241001486992 Taiwanofungus camphoratus Species 0.000 title claims description 96
- 238000004519 manufacturing process Methods 0.000 title 1
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- XBZYWSMVVKYHQN-MYPRUECHSA-N (4as,6as,6br,8ar,9r,10s,12ar,12br,14bs)-10-hydroxy-2,2,6a,6b,9,12a-hexamethyl-9-[(sulfooxy)methyl]-1,2,3,4,4a,5,6,6a,6b,7,8,8a,9,10,11,12,12a,12b,13,14b-icosahydropicene-4a-carboxylic acid Chemical compound C1C[C@H](O)[C@@](C)(COS(O)(=O)=O)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CCC(C)(C)C[C@H]5C4=CC[C@@H]3[C@]21C XBZYWSMVVKYHQN-MYPRUECHSA-N 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
Description
本發明係關於一種牛樟芝萃取方法，特別是一種提升馬偕一號之溶出率的牛樟芝萃取方法。The invention relates to an extraction method of Antrodia camphorata, in particular to an extraction method of Antrodia camphorata which improves the dissolution rate of Majing No.1.
牛樟芝(Antrodia cinnamomea )為台灣特有珍貴藥材，僅生長於台灣保育類之牛樟樹(Cinnamomum kanehirai )的中空芯材內壁，野生牛樟芝含有豐富的三萜類(Triterpenoids)，三萜類具有抑制癌症細胞生長、修復肝臟及促進肝功能、降低血脂及血壓、提升免疫能力等功能。 Antrodia cinnamomea is a unique and precious medicinal material in Taiwan. It is grown only on the inner wall of hollow core material of Cinnamomum kanehirai , which is rich in triterpenoids. Triterpenoids have inhibitory cancer cells. The function of growing, repairing the liver and promoting liver function, lowering blood lipids and blood pressure, and improving immunity.
其中，馬偕一號(dehydrosulphurenic acid)係為牛樟芝特有之三萜類成分，馬偕一號隸屬於羊毛甾烷固醇類化合物(lanostane)，為生物體細胞生理代謝過程中，經由角鯊烯(squalene)合成固醇(sterol)之中間產物，因而容易干擾生物體的膽固醇代謝及細胞膜生成的生化路徑，對於細胞成長週期及生理代謝具有顯著之影響。研究中亦發現，馬偕一號係具有可以促進癌症細胞之凋亡及有絲分裂風暴(mitotic catastrople)之功效，對於治療胰臟癌及骨髓性急性白血病具有極佳之效果。Among them, dehydrosulphurenic acid is a triterpenoid unique to burdock, and 偕1 is a lanostane, which is a physiological metabolic process of organisms via squalene. (squalene) is an intermediate product of synthetic sterol, which easily interferes with the biochemical pathway of cholesterol metabolism and cell membrane formation in organisms, and has a significant influence on cell growth cycle and physiological metabolism. The study also found that Majing No.1 has the effect of promoting apoptosis and mitotic catastrople of cancer cells, and has excellent effects in the treatment of pancreatic cancer and myeloid acute leukemia.
為了提高牛樟芝之營養價值，習知牛樟芝萃取方法係使牛樟芝所含之三萜類溶出於萃取溶劑，並且經由濃縮及乾燥，可以獲得一具有高濃度之營養成分的牛樟芝萃取物，使用者僅需服用少量之該牛樟芝萃取物，即可以獲得等同於服用大量牛樟芝相等之效果。In order to improve the nutritional value of Antrodia camphorata, the extraction method of Antrodia camphorata is such that the triterpenoids contained in Antrodia camphorata are dissolved in an extraction solvent, and concentrated and dried to obtain an extract of Antrodia camphorata having a high concentration of nutrients, which is only required by the user. Taking a small amount of the Antrodia camphorata extract can achieve the same effect as taking a large amount of Antrodia camphor.
習知牛樟芝萃取方法中，係依據萃取溶劑之選擇，區分為水萃取法及有機溶劑萃取法。In the extraction method of the known Antrodia camphorata, it is divided into a water extraction method and an organic solvent extraction method according to the selection of the extraction solvent.
習知水萃取法，係以水作為萃取溶劑，對一牛樟芝生藥材進行萃取，以獲得一牛樟芝水萃物；然而，由於三萜類之水溶性不佳，因而該牛樟芝水萃物所含有之三萜類總含量普遍不高。The conventional water extraction method uses water as an extraction solvent to extract a raw material of Antrodia camphorata to obtain an aqueous extract of Antrodia camphorata; however, since the water solubility of the triterpenoids is not good, the aqueous extract of Antrodia camphorata contains The total content of the triterpenoids is generally not high.
再者，習知有機溶劑萃取法，係以如醇類、酯類、烷類及鹵烷類等有機溶劑作為萃取溶劑浸潤該牛樟芝生藥材，接著以低溫蒸餾去除該有機溶劑，以獲得一牛樟芝萃取物；相較於習知水萃取法，雖然以習知有機溶劑萃取法所萃得之三萜類總含量較高，但是，卻仍無法有效提升馬偕一號之溶出量。Furthermore, a conventional organic solvent extraction method is used to infiltrate the raw material of Antrodia camphorata with an organic solvent such as an alcohol, an ester, an alkane or a halogenated alkane as an extraction solvent, and then remove the organic solvent by low temperature distillation to obtain a The extract of Antrodia camphorata; compared with the conventional water extraction method, although the total content of the triterpenoids extracted by the conventional organic solvent extraction method is high, it still cannot effectively improve the dissolution amount of the Majing No.1.
本發明之主要目的係提供一種牛樟芝萃取方法，係可以提升馬偕一號之溶出率，以獲取具有較高馬偕一號含量之牛樟芝萃取物者。The main object of the present invention is to provide an extraction method of Antrodia camphorata, which can improve the dissolution rate of Majing No.1 to obtain an extract of Antrodia camphorata having a higher content of Maji No.1.
為達到前述發明目的，本發明所運用之技術手段及藉由該技術手段所能達到之功效包含有：一種牛樟芝萃取方法，係包含：將一牛樟芝生藥材浸漬於2%之一氯化鈉水溶液，使該牛樟芝生藥材吸收該氯化鈉水溶液，以獲得一浸漬牛樟芝；及將該浸漬牛樟芝浸泡於一醇類溶劑，經由超音波震盪，以獲得一牛樟芝萃取物。In order to achieve the foregoing object, the technical means and the effects achievable by the technical method include: a method for extracting Antrodia camphorata, comprising: immersing a raw material of Antrodia camphorata in 2% of one sodium chloride The aqueous solution is used to absorb the aqueous solution of sodium chloride to obtain an impregnated Antrodia camphorata; and the impregnated Antrodia camphorata is immersed in an alcohol solvent and oscillated by ultrasonic waves to obtain an extract of Antrodia camphorata.
本發明之牛樟芝萃取方法，其中，另包含將該浸漬牛樟芝以醇類溶劑浸泡萃取之前，將該浸漬牛樟芝置於一密閉空間，於溫度為110至150℃，壓力為1至3kg/cm2 之環境下加熱蒸煮。The method for extracting Antrodia camphorata according to the present invention, further comprising: placing the impregnated Antrodia camphorata in a closed space at a temperature of 110 to 150 ° C and a pressure of 1 to 3 kg/cm 2 before the impregnated Antrodia camphorata is soaked and extracted with an alcohol solvent ; Heat and cook under the environment.
本發明之牛樟芝萃取方法，其中，係先清除該牛樟芝生藥材之表面雜質後，再將該牛樟芝生藥材浸漬於該氯化鈉水溶液中。In the method for extracting Antrodia camphorata according to the present invention, the surface impurities of the raw material of the Antrodia camphorata are first removed, and then the raw material of the Antrodia camphorata is immersed in the aqueous solution of sodium chloride.
本發明之牛樟芝萃取方法，其中，加熱蒸煮時間為15至60分鐘。The method for extracting Antrodia camphorata according to the present invention, wherein the heating and cooking time is 15 to 60 minutes.
本發明之牛樟芝萃取方法，其中，係於溫度為121℃，壓力 為1kg/cm2 之環境下加熱蒸煮。The method for extracting Antrodia camphorata according to the present invention is characterized in that it is heated and cooked in an environment having a temperature of 121 ° C and a pressure of 1 kg / cm 2 .
本發明之牛樟芝萃取方法，其中，係將500克牛樟芝生藥材浸漬於1公升的氯化鈉水溶液中。The method for extracting Antrodia camphorata according to the present invention, wherein 500 g of the raw material of Antrodia camphorata is immersed in a 1 liter aqueous solution of sodium chloride.
本發明之牛樟芝萃取方法，其中，該醇類溶液係為95%之乙醇溶劑。The method for extracting Antrodia camphorata according to the present invention, wherein the alcohol solution is a 95% ethanol solvent.
本發明之牛樟芝萃取方法，其中，該牛樟芝生藥材係選自牛樟芝子實體。The method for extracting Antrodia camphorata according to the present invention, wherein the raw material of Antrodia camphorata is selected from the group consisting of Antrodia camphorata.
本發明之牛樟芝萃取方法，經由該鹽類溶液之浸漬後，可以提升馬偕一號於該醇類溶劑之釋出量，進而達到獲得含有較高含量之馬偕一號的牛樟芝萃取物之功效。The method for extracting Antrodia camphorata according to the present invention can enhance the release amount of Majing No. 1 in the alcohol solvent after being immersed in the salt solution, thereby achieving the effect of obtaining the extract of Antrodia camphorata containing a high content of Ma Rong No.1. .
第1A圖係第A1組牛樟芝萃取物以高效液相層析法分析之結果。Fig. 1A shows the results of analysis of the extract of Antrodia camphorata in Group A1 by high performance liquid chromatography.
第1B圖係第A2組牛樟芝萃取物以高效液相層析法分析之結果。Figure 1B shows the results of analysis of the extract of A. striata in Group A2 by high performance liquid chromatography.
第1C圖係第A3組牛樟芝萃取物以高效液相層析法分析之結果。Fig. 1C shows the results of analysis of the extract of Antrodia camphorata in Group A3 by high performance liquid chromatography.
第1D圖係第A4組牛樟芝萃取物以高效液相層析法分析之結果。Fig. 1D shows the results of analysis of the extract of A. striata in Group A4 by high performance liquid chromatography.
第1E圖係第A5組牛樟芝萃取物以高效液相層析法分析之結果。Fig. 1E shows the results of analysis of the extract of the A5 group of A. angustifolia by high performance liquid chromatography.
為讓本發明之上述及其他目的、特徵及優點能更明顯易懂，下文特舉本發明之較佳實施例，並配合所附圖式，作詳細說明如下：本發明之牛樟芝萃取方法，係包含以下步驟：將一牛樟芝生藥材浸漬於一鹽類溶液，使該牛樟芝生藥材吸收該鹽類溶液，以獲得一浸漬牛樟芝；及將該浸漬牛樟芝浸泡於一醇類溶液，經由超音波震盪，以獲 得一牛樟芝萃取物。The above and other objects, features, and advantages of the present invention will become more apparent from the aspects of the invention. The method comprises the steps of: immersing a raw material of a burdock in a salt solution, so that the raw material of the burdock medicinal material absorbs the salt solution to obtain a immersed Antrodia camphorata; and immersing the immersed Antrodia camphorata in an alcohol solution through ultrasonic wave Concussion Get an extract of Antrodia camphorata.
詳而言之，係將定量之牛樟芝生藥材浸漬於定量之鹽類溶液中，以獲得浸漬牛樟芝。其中，本實施例係可以先清除該牛樟芝生藥材表面之雜質後，再浸漬於該鹽類溶液中，以避免該牛樟芝生藥材表面之雜質影響後續萃取之效果。該鹽類溶液係可以選擇為重量百分比為2%之氯化鈉，氯化鈉容易取得，價錢便宜，因而可以降低成本，藥物經食鹽水製後能改變藥物的性能，增強藥物的作用。本實施例係將該牛樟芝生藥材浸漬於該氯化鈉水溶液中，且較佳係於一封閉容器中浸漬，以避免外界雜質進入該氯化鈉水溶液中。如此，便可透過該氯化鈉水溶液悶透滲入該牛樟芝中，進而共同形成該浸漬牛樟芝。舉例而言，本實施例係選擇以500克之牛樟芝生藥材經去除表面雜質後，浸漬於該一公升之氯化鈉水溶液中24小時以獲得該浸漬牛樟芝。In detail, the quantitative Astragalus membranaceus medicinal material is immersed in a quantitative salt solution to obtain impregnated Antrodia camphorata. In this embodiment, the impurities on the surface of the raw material of the burdock raw medicinal material can be removed first, and then immersed in the salt solution to avoid the effect of the impurities on the surface of the raw medicinal material of the burdock. The salt solution can be selected as sodium chloride in a percentage by weight of 2%, sodium chloride is easy to obtain, and the price is low, so that the cost can be reduced, and the drug can change the performance of the drug after being made of saline, and enhance the effect of the drug. In this embodiment, the raw material of Antrodia camphorata is immersed in the aqueous solution of sodium chloride, and is preferably immersed in a closed container to prevent external impurities from entering the aqueous solution of sodium chloride. In this way, the sodium chloride aqueous solution can be smothered and infiltrated into the burdock, and the impregnated burdock can be formed together. For example, in the present embodiment, after 500 g of the raw material of Antrodia camphorata was removed, the surface impurities were removed, and then immersed in the one-liter aqueous solution of sodium chloride for 24 hours to obtain the impregnated Antrodia camphorata.
接著，係取浸漬牛樟芝，浸泡於醇類溶劑，經由超音波震盪，以獲得牛樟芝萃取物；本較佳實施例中，該醇類溶劑係可以選擇為濃度為95%之乙醇溶劑，係可以提萃出較高含量之三萜類，並且，乙醇溶劑係可以與無機成分形成結晶狀，且對水溶解度高之分子化合物，因而固可以提高其溶解度；較佳地，係可以取5克該浸漬牛樟芝，置於600毫升之該乙醇溶劑，於25℃環境下，以頻率為40KHz之超音波進行震盪萃取，使該浸漬牛樟芝中的有效成份(如三萜類)可以溶出於該乙醇溶劑；較佳地，該萃取時間係為8小時，並重複萃取3次，以提高總萃取量，以獲得該牛樟芝萃取物；另外亦可以再經由冷凍乾燥(-55℃，真空度0torr)以獲得一濃縮之牛樟芝萃取物。Then, the impregnated Antrodia camphorata is immersed in an alcohol solvent and oscillated by ultrasonic waves to obtain an extract of Antrodia camphorata; in the preferred embodiment, the alcohol solvent may be selected as a solvent having a concentration of 95%, which may be mentioned. A higher content of triterpenoids is extracted, and the ethanol solvent is a molecular compound which can form a crystal with an inorganic component and has high solubility in water, thereby improving the solubility thereof; preferably, 5 g of the impregnation can be taken. Antrodia camphorata, placed in 600 ml of the ethanol solvent, is subjected to shock extraction at a frequency of 40 kHz at 25 ° C, so that the active ingredients (such as triterpenoids) in the impregnated Antrodia camphorata can be dissolved in the ethanol solvent; Preferably, the extraction time is 8 hours, and the extraction is repeated 3 times to increase the total extraction amount to obtain the Antrodia camphorata extract; or it can be further lyophilized (-55 ° C, vacuum degree 0 torr) to obtain a concentration. Antrodia camphora extract.
較佳地，本發明之牛樟芝萃取方法更可以包含將浸漬牛樟芝以醇類溶劑浸泡萃取之前，將該浸漬牛樟芝置於一密閉空間，於溫度為110至150℃，壓力為1至3kg/cm2 之環境下加熱蒸煮，可以促進馬偕一號之 溶出。更詳言之，本實施例係選擇以可控制高溫高壓環境之裝置或儀器(例如壓力鍋)提供該密閉空間，以控制該密閉容器內之壓力及溫度。本實施例係將該浸漬牛樟芝置放於該可控制高溫高壓環境之壓力鍋，環境溫度控制為110至150℃，環境壓力控制為1至3Kg/cm2 ，進行蒸煮15分鐘至1小時。其中，若溫度低於110℃，牛樟芝之三萜類被萃取出之效率不佳；若溫度高於150℃，則可能因高溫而破壞牛樟芝中之活性成分。而欲達到110℃至150℃之高溫，則需要透過該密閉空間提供一壓力為1至3Kg/cm2 之環境，若壓力低於1Kg/cm2 ，則蒸汽溫度不易提升；若壓力大於3Kg/cm2 ，則蒸汽溫度可能過高。舉例而言，本實施例係於121℃，1Kg/cm2 環境條件下進行蒸煮15至60分鐘，使該浸漬牛樟芝外觀顏色加深呈現黑紅色，即獲得該蒸透之浸漬牛樟芝，以利再以該醇類溶劑浸泡萃取，以獲得該牛樟芝萃取物。如此，透過於該密閉空間內進行蒸煮，使蒸汽無法外洩，不但容易控制溫度壓力，且可透過該密閉空間提供高壓環境以提升水之沸點，進而提升該蒸汽之溫度至超過110℃。因此，便可以高溫高壓之蒸汽於該密閉空間內對該浸漬牛樟芝進行蒸煮，而僅需短時間便可將該浸漬牛樟芝蒸透至表面呈現黑紅色，大幅縮短蒸煮時間，進而提升效率，降低成本。Preferably, the method for extracting Antrodia camphorata according to the present invention may further comprise placing the impregnated Antrodia camphorata in a confined space at a temperature of 110 to 150 ° C and a pressure of 1 to 3 kg/cm 2 before soaking the impregnated Antrodia camphorata in an alcohol solvent. Heating and cooking in the environment can promote the dissolution of Ma Rong No.1. More specifically, the present embodiment selects a sealed space in a device or apparatus (e.g., a pressure cooker) that can control a high temperature and high pressure environment to control the pressure and temperature within the closed container. In this embodiment, the impregnated Antrodia camphorata is placed in the pressure cooker capable of controlling a high temperature and high pressure environment, the ambient temperature is controlled to be 110 to 150 ° C, the ambient pressure is controlled to be 1 to 3 Kg/cm 2 , and cooking is performed for 15 minutes to 1 hour. Among them, if the temperature is lower than 110 ° C, the triterpenoids of Antrodia camphorata are extracted with poor efficiency; if the temperature is higher than 150 ° C, the active ingredients in Antrodia camphorata may be destroyed due to high temperature. In order to reach a high temperature of 110 ° C to 150 ° C, it is necessary to provide a pressure of 1 to 3 Kg / cm 2 through the closed space, if the pressure is less than 1 Kg / cm 2 , the steam temperature is not easy to increase; if the pressure is greater than 3 Kg / Cm 2 , the steam temperature may be too high. For example, the present embodiment is cooked at 121 ° C, 1 Kg / cm 2 ambient conditions for 15 to 60 minutes, so that the appearance of the impregnated Antrodia camphora is dark red, that is, the steamed impregnated Antrodia camphorata is obtained, so as to obtain The alcohol solvent is soaked and extracted to obtain the Antrodia camphorata extract. In this way, by cooking in the sealed space, the steam can not be leaked, and the temperature and pressure can be easily controlled, and a high-pressure environment can be provided through the sealed space to raise the boiling point of the water, thereby increasing the temperature of the steam to over 110 °C. Therefore, the impregnated Antrodia camphorata can be cooked in the confined space by high-temperature and high-pressure steam, and the impregnated Antrodia camphorata can be vaporized to a black-red surface in a short time, thereby greatly shortening the cooking time, thereby improving efficiency and reducing cost. .
為證實本實施例之牛樟芝萃取方法係可以提升該牛樟芝萃取物之馬偕一號之含量，係以高效液相層析法(HPLC)進行分析。In order to confirm that the extracting method of the Antrodia camphorata in the present embodiment can enhance the content of the Astragalus membranaceus extract, the content of the horse 偕1 is analyzed by high performance liquid chromatography (HPLC).
詳而言之，本試驗係可以取如第1表所示之牛樟芝萃取物，第A1組係為牛樟芝生藥材經過該乙醇溶劑浸泡萃取獲得之牛樟芝萃取物，第A2組係為浸漬牛樟芝經由該乙醇溶劑浸泡萃取獲得之牛樟芝萃取物，第A3至A5組係為浸漬牛樟芝，分別經由15、30及60分鐘之蒸煮後，再以進行乙醇溶劑浸泡萃取，所獲得之牛樟芝萃取物。分別秤取0.2克之該等牛樟芝萃取物至一螺旋試管中，加入5毫升甲醇，以超音波震盪15分 鐘，再以3000轉離心10分鐘後，取5毫升上清液，置入一乾淨試管，再以100℃水浴加熱至乾燥。In detail, the test can take the extract of Antrodia camphorata as shown in Table 1, the group A1 is the extract of Antrodia camphorata obtained by soaking and extracting the raw material of Antrodia camphorata, and the A2 group is impregnated with Antrodia camphorata. The extract of Antrodia camphorata obtained by soaking and extracting in an ethanol solvent, and the A3 to A5 group are impregnated Antrodia camphorata, which are respectively cooked for 15, 30 and 60 minutes, and then subjected to ethanol solvent soaking and extraction to obtain the extract of Antrodia camphorata. Weigh 0.2 g of these Antrodia camphorata extracts into a spiral test tube, add 5 ml of methanol, and vortex 15 points with ultrasonic waves. After centrifugation for 10 minutes at 3000 rpm, 5 ml of the supernatant was taken, placed in a clean tube, and heated to dryness in a 100 ° C water bath.
接著選用Purospher STAR(Merck)RP-18e(5μm)250mm×4.6mm管柱行分析，以如第2表所示之乙腈(Acetonitrile，簡稱ACN)及0.085%之磷酸溶液沖提溶液作為流動相，流速為1ml/min，偵測波長為254nm之吸光值以進行後續分析。Then, using a Purospher STAR (Merck) RP-18e (5 μm) 250 mm × 4.6 mm column, the solution was extracted as a mobile phase with an acetonitrile (Acetonitrile, ACN for short) and a 0.085% phosphoric acid solution as shown in Table 2. The flow rate was 1 ml/min, and the absorbance at a wavelength of 254 nm was detected for subsequent analysis.
請參照第1A至1E圖及第3表所示，相較於第A1組，牛樟芝生藥材經該氯化鈉水溶液浸漬後(第A2組)，經由該乙醇溶劑浸泡萃取獲得之牛樟芝萃取物中，樟芝酸K(antcin K)、樟芝酸C(antcin C)、樟菇酸C(zhankuic acid C)、樟菇酸A(zhankuic acid A)與雲鵬1號(dehydroeburicoic acid)之釋出量均有減低的趨勢，但是馬偕1號(dehydrosulphurenic acid)的釋出率相較於第A1組則有約4倍之增加。再者，經該氯化鈉水溶液浸漬，並經由高溫蒸煮15分鐘，經由該乙醇溶劑浸泡萃取獲得之牛樟芝萃取物(第A3組)內之馬偕1號的釋出量提升為第A1組之6倍；蒸煮時間延長為30分鐘(第A4組)，馬偕1號的釋出量為第A1組之5.5倍；蒸煮時間為60分鐘(第A5組)，馬偕1號的釋出量為第A1組之3倍。Referring to Figures 1A to 1E and Table 3, compared with Group A1, the raw material of Antrodia camphorata is immersed in the aqueous solution of sodium chloride (Group A2), and the extract of Antrodia camphorata obtained by soaking and extracting in ethanol solvent In the middle, the release of K (antcin K), antinc C, zhankuic acid C, zhankuic acid A and dehydroeburicoic acid The yield was reduced, but the release rate of dehydrosulphurenic acid was about 4 times higher than that of group A1. Further, after being immersed in the sodium chloride aqueous solution and being cooked at a high temperature for 15 minutes, the release amount of the horse 偕1 in the extract of the Antrodia camphorata (Group A3) obtained by the ethanol solvent soaking and extraction is raised to the group A1. 6 times; cooking time is extended to 30 minutes (Group A4), release amount of Majing No. 1 is 5.5 times of Group A1; cooking time is 60 minutes (Group A5), release amount of Majing No.1 It is 3 times of the A1 group.
由此可知，本較佳實施例之牛樟芝萃取方法中，透過以該鹽類溶液之浸漬，係可以顯著增加馬偕一號之釋放量，並且，再輔以高溫、高壓之蒸煮，更能益於助馬偕一號之釋出，其中，蒸煮之時間係以15分鐘為最佳。It can be seen that in the extraction method of the Antrodia camphorata of the preferred embodiment, the impregnation with the salt solution can significantly increase the release amount of the Mazong No. 1, and is supplemented by cooking at a high temperature and a high pressure, which is more beneficial. It was released on the first day of the horse, and the cooking time was best in 15 minutes.
綜合上述，本發明之牛樟芝萃取方法，經由該鹽類溶液之浸漬後，可以提升馬偕一號於醇類溶劑之釋出量，進而達到獲得含有較高含量之馬偕一號的牛樟芝萃取物之功效。In summary, the method for extracting Antrodia camphorata according to the present invention can enhance the release amount of the Maji No. 1 in an alcohol solvent after being immersed in the salt solution, thereby obtaining an extract of Antrodia camphorata containing a high content of Ma Rong No.1. The effect.
雖然本發明已利用上述較佳實施例揭示，然其並非用以限定本發明，任何熟習此技藝者在不脫離本發明之精神和範圍之內，相對上述實施例進行各種更動與修改仍屬本發明所保護之技術範疇，因此本發明之保護範圍當視後附之申請專利範圍所界定者為準。While the invention has been described in connection with the preferred embodiments described above, it is not intended to limit the scope of the invention. The technical scope of the invention is protected, and therefore the scope of the invention is defined by the scope of the appended claims.
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