TWI379642B - - Google Patents

Description

1379642 (1) Description of the Invention [Technical Field] The present invention relates to a method for controlling a disease caused by a disease occurring during rice seedling raising period and a disease caused by a rice seedling period using the same. [Prior Art] For rice cultivation, one of the most important operations for cultivating a sound and homogeneous rice seedling system is one of the most important operations in the seedling stage, but it is easy to suffer from various diseases during the germination to the seedling stage during the seedling period, and is usually completely prevented by chemical agents. Traditionally used as a chemical synthetic agent for disease control during rice seedling raising, for example, for rice seedlings caused by filamentous fungal infections, rice fever, rice leaf blight, etc., DMI (Dimethyl) such as triflumizole is generally used. Itaconate, dimethyl acetonate, widely used oxolinic acid due to bacterial bacterial diseases, rice bacterial blight, rice seedling bacterial disease, rice seedling blight, rice brown stalk Oxolinic acid) et al. In addition, the rice seedling blight of the soil infectious disease is controlled by a drug containing hydroxy isoxazole' Benzomyl (free). · However, there is a problem that pathogens with low sensitivity or resistance to the above chemical synthesis agents appear. In addition, because of concerns about the safety of food or the environment, it is required to reduce the amount of chemical synthetic agents used or the number of uses for crop cultivation. Further, as the demand for food safety and the like is increased, it is required to develop a technique/commodity that can reduce the amount of use or the number of times of use of chemically synthesized chemicals in the crop seedling period. As such a technique

c S -4 - (2) (2) 1379642. Specifically, it can be used as a highly safe chemical or a biological pesticide for use as a natural product or a food additive. As a disease control technique using a living organism, it is known to control by a microbial preparation. For example, Patent Document 1 describes a microbial preparation for controlling rice diseases containing Fusarium bacteria, and Patent Document 2 describes a microbial preparation for controlling rice seedling diseases containing Pseudomonas. In addition, Biotrust (Icosu Kosan Co., Ltd.), a microbial fungicide with a filamentous fungus of Talaromyces flavus, is used for controlling stem and leaf diseases of strawberry caries and strawberry powdery mildew. Agent. However, Talaromyces has a disease-preventing effect on other crops and has not been reported so far. [Patent Document 1] Japanese Laid-Open Patent Publication No. Hei. No. Hei. No. Hei. No. Hei. No. Hei. It is a problem that microbial pesticides with excellent disease control effects and safety and little environmental impact are produced. In order to solve the above-mentioned problems, the inventors of the present invention have found that the filamentous fungi belonging to the genus Talaromyces have many control effects on rice seedlings during the seedling raising period, and have achieved the control effect based on these findings. invention. <5 -5- (4) (4) 1379642 is a control method for diseases caused during rice seedling raising period. BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in detail below. (Disease Control Agent of the Present Invention) The disease control agent of the present invention is an agent for preventing diseases occurring during rice seedling raising. Here, the so-called "miao" refers to the young plant body that is transplanted into rice. In addition, the so-called "nurturing" refers to the cultivation of rice seedlings. The so-called "nurturing period" refers to the cultivation period before the rice seedlings are inserted. The number of leaves of the rice seedlings in the 1st leaf stage, 2 leaf stage and 3 leaf stage in the cultivation period indicates the growth period of the rice seedlings. When it comes to the growth period of rice seedlings, the rice seedlings in the so-called seedling stage, such as seedling transplanting, are suitable for transplanting in the four-leaf stage of incomplete leaves. The disease control agent of the present invention contains a filamentous fungus belonging to the genus Talaromyces as an active ingredient. (1) A filamentous fungus belonging to the genus of the genus Bacillus, which is a filamentous fungus belonging to the genus of the genus Bacillus, as long as it is a pathogenic bacterium which causes disease in the rice seedling raising period, and has a pest resistance effect, and there is no particular limits. Here, the term "extraction resistance" refers to the effect of reducing the number of cells or inhibiting the proliferation of a pathogenic bacteria causing a disease during the rice seedling raising period, that is, an antibacterial action. As a pathogen which causes disease in the rice seedling raising period, the bacteria having an anti-allergic action may, for example, be (3) (6) 1379642, preferably 3 to 60 days, and preferably 3 to 20 days. Further, in the case of the filamentous fungus belonging to the genus Bacillus, which is used in the present invention, spores are preferred from the viewpoint of the preservation of the disease controlling agent product. Therefore, in order to sporulate the filamentous fungus belonging to the genus Bacillus, if necessary, at the end of the culture, the culture conditions such as the composition of the medium, the culture temperature, the culture humidity, and the oxygen concentration during the culture are adjusted to suit the spore. The formation conditions are suitable. The liquid medium component to be used for the filamentous fungus belonging to the genus of the genus genus is used, and the potato dextrose medium or the Sabo uraud medium can be used. When solid culture is carried out, solid materials derived from cereals such as rice, wheat, maize, soybean, etc., solid materials derived from cereals, or clay minerals containing nutrient sources can be used as needed. A medium containing a sugar or a nitrogen source or the like. A culture containing a filamentous fungus belonging to the genus A. genus can be directly used. The culture can also be broken or chopped as needed. Further, the culture can be used to recover spores from a sieve or the like. . In addition, the cells may be isolated from the culture by using a liquid such as water or oil, and the disease control agent of the present invention may be contained as a usual colony forming unit, usually 1 M06 to 1×1 2 2 cfu/g. 1χΐ〇7 to ixio^cfu/g is a pulverized material of a culture or culture of a filamentous fungus belonging to the genus genus, and is formulated into various preparations suitable for use or use. The disease controlling agent of the present invention contains the content of the pulverized material of the culture or the culture of the filamentous fungus belonging to the genus genus of the genus genus of the genus of the genus of the genus of the genus of the genus of the genus of the genus of the genus of the genus of the genus of the genus good. -9- (7) (7) 1379642 (2) Arbitrary component In addition to the above-mentioned filamentous fungus belonging to the genus of the genus genus, the disease controlling agent of the present invention can be used as an optional component as long as it does not impair the effects of the present invention. And add to use. Any such component may be contained for the purpose of formulation, quality stabilization, and the like. Any component used as the disease control agent of the present invention may be used in combination of one or more of the following components. For example, as a bulking agent, a solid carrier clay, pyrophyllite, bentonite, montmorillonite, diatomaceous earth, synthetic aqueous cerium oxide, acid white clay, talc, clay, ceramic, quartz, sericite, strontium can be used. Mineral powders of stone, bead iron, aoki, anthracite, limestone, coal, zeolite, etc.; inorganic compounds such as salt, carbonate, sulfate, nitrate, urea; rice husks, clams, crab shells, shrimp shells , krill micro powder, rice bran, wheat flour, corn cob, groundnut shell, bone meal, fish meal, lotus root starch, wood flour, carbon, smoked carbon, bark carbon, rice husked carbon, grass carbon, muddy moss, green Micro-powder of organic matter such as palygorskite, dried manure, activated carbon, oil mash, starch and hydrolyzate thereof; soluble extender such as sorbitan, lactose, maltose, glucosamine, oligosaccharide, and the like. A liquid carrier water, vegetable oil, animal oil, mineral oil, synthetic water-soluble polymer, or the like can be used. Further, for the purpose of adhesion, adhesion, dispersion, etc., as a supplementary agent, casein, gelatin 'arabin gum', alginic acid 'cellulose, carboxymethyl cellulose, sinosaur, chitin, etc. may be contained as needed. Natural polysaccharides such as chitosan; polyvinyl alcohols; polyacrylic acids; bentonites, etc.

C S -10- (8) (8) 1379642 Further, for the purpose of preventing freezing, etc., a glycol such as ethylene glycol or propylene glycol may be contained as needed. For the purpose of dispersing stability, preventing aggregation, emulsification, etc., an anionic, cationic or amphoteric surfactant may be contained as needed. (Method for Producing Disease Control Agent of the Present Invention) The disease control agent containing the filamentous fungus belonging to the genus A. genus of the present invention can be formulated into a form which is practically easy to use. That is, it can be formulated into a wettable powder, a powder, a granule, an emulsion, a suspending agent, a coating agent and the like according to the usual method for producing the preparation, as needed, with various optional ingredients. For example, a wettable powder or a powder is applied to a solid carrier as described above, and if necessary, it can be produced by mixing or pulverizing and mixing a surfactant or a stable component as described above. For example, the granules may be produced on a solid carrier as described above, if necessary, by mixing or pulverizing a surfactant such as the above-mentioned surfactant or a stable quality component, followed by granulation. For example, the emulsion is a liquid carrier such as vegetable oil, animal oil, mineral oil, or the like, and may be produced by emulsifying or dispersing a surfactant such as the above, and mixing or pulverizing and mixing a stable quality component, if necessary. For example, the suspending agent is for the purpose of preventing the freezing of the above-mentioned diol or the like for the purpose of preventing the freezing of the above-mentioned diol, etc., for the purpose of dispersing the above-mentioned surfactant, etc., and by mixing or pulverizing and mixing. Made of quality ingredients. For example, the coating is added to a liquid carrier such as water or oil, and the -11 - (9) (9) 1397642 agent is added and mixed, and it can be produced by forming a sol or a gel. (Method of Application of Disease Control Agent of the Present Invention) The disease control agent of the present invention is effective for controlling diseases caused by rice seedlings by filamentous bacteria, bacteria, microbial vector viruses and the like. Further, the disease controlling agent of the present invention is effective for controlling, for example, a disease caused by a rice seed infectious disease or a soil infectious disease. More specifically, the disease control agent of the present invention is particularly effective for, for example, Gibberella fujikuroi, Pyricularia oryzae, Cochliobolus miyabeanus, and rice bacterial blight ( Pseudomonas glumae), Pseudomonas plantarii, Pseudomonas avenae, rice seedling blight (Fusarium, Pythium, Rhizopus) Control of diseases caused by Rhizopus, Trichoderma, etc. The disease control agent of the present invention acts on one or more of the above pathogenic bacteria. In the above-mentioned pathogens causing diseases in the rice seedling raising period, not only the rice seedling raising period but also the pathogenic bacteria causing the disease during the period other than the seedling raising period, the so-called "pathogenic bacteria causing the disease during the rice seedling raising period" means at least rice. The pathogen causing the disease during the nursery period also includes any pathogenic bacteria causing the disease at a period other than the nursery period, and a pathogen which does not cause disease except for the period of the nursery period. "The disease of the present invention containing the filamentous fungus belonging to the genus Eucalyptus The control agent -12-(10) 1379642 is applied to rice seeds, rice seedlings, seedling soils or seedling culture medium for the purpose of preventing various diseases as described above, and the method is appropriately selected depending on the use form or disease of the dosage form or the like. As such a method, for example, seed immersion treatment, seed coating treatment, seed coating treatment, seed dispersion (including spray mist) treatment, soil dispersion (including spray) treatment, soil mixed application, soil perfusion application, seedling box perfusion Application, root application, above-ground liquid dispersion, above-ground solid dispersion, and the like. % In addition, when applying a disease control agent for rice, it may be mixed with a bactericide, an insecticide, a nematicide 'acaricide, a herbicide, a plant growth regulator, a fertilizer, a soil improvement material, or the like, or may be mixed without Apply interactively, or simultaneously. The application amount of the disease control agent containing the filamentous fungus belonging to the genus of the genus of the genus of the genus of the genus of the genus of the genus of the genus of the genus of the genus of the genus of the genus of the genus of the genus of the genus of the genus of the genus of the genus Up to 1000 times (mass) is suitable for the immersion liquid per 1 ml of the concentration of the bacteria, usually _ 1 χ 103 to lxl01 () Cfu, preferably lxl 〇 4 to lxi09 cfU. ' When the seed coating is treated, it is appropriate to apply 1 to 20% by mass of the preparation relative to the seed quality. The concentration of the bacteria is per lg of seed mass, usually - lxio3 to lxl01()cfu' to 1M04 to lxl 〇9cfu is appropriate. - When soil is applied, it is usually used as a seedling box for seedling transplanting (for example, an area of about 1 800 cm2). If it is dispersed as a liquid, it is suitable to apply 5 to 1000 ml, and the concentration of the cell is distributed per iml. The liquid, usually lxlO3 to lxlOl()cfu' is preferably lxi〇4 to ixio'fu. When the soil is mixed and applied, it is usually used as a seedling for seedling transplanting.

C S -13- (13) 1379642 hours 'The seed was treated as a medicament. In addition, the rice seedling seed was similarly immersed in water, and it was used as a control (nowhere (3) cultivation test plant, the above-mentioned immersed rice seedling pathogen was infected with 100 cm2 of plastic for the rice seedling culture soil. The area where the seed treatment of the seed is used as the chemical treatment area, and the area to be sown is used as the no treatment area. The chemical treatment area and the non-treatment area: After the soil is covered with the above-mentioned rice seedling culture soil, it is cultivated and managed in a glass temperature method. (4) Investigation and control effect After 2 weeks of sowing, it was found that the rice seedlings which were caused by the long-term disease of the rice seedlings were used as the seedlings, and the average sputum was repeated three times in each treatment zone, and the seedling rate was calculated based on the following (Formula 1). Based on the following (Formula 2), the drug devaluation is calculated as the control effect. Incidence seedling rate (%) = number of diseased seedlings / total number of investigated seedlings xlOO (: control price = [(no treatment area incidence rate - drug treatment area / The incidence of seedlings in the no treatment zone] X100 (Formula 2) (5) The results of the investigation are shown in Table 1. As shown in Table 1, the long pathogens infected the seeds. Seeds, seeding and seedling boxes. 3 The number of vaccines in the room is determined by the number of vaccines in the treatment area. The disease prevention rate of the treatment area is 1) The disease prevention factor of the present invention-16-(14)1379642 Significantly high control effect. The first table treatment area dilution ratio rice seedlings long disease incidence rate control rate without treatment area • 55.7% - preparation example 1 treatment area 200 3.0% 94.7 <Example 2> (rice rice fever control test) (1) modulation infection The seeds are harvested from the rice (variety · Akita Komachi) seeds of rice fever that often occurs in the field. This seed is used as a seed for rice fever infection. (2) The drug treatment was diluted with water to prepare a 200-fold (mass) of the preparation prepared in the above Preparation Example 1, and in 15 ml of this solution, 4 g of the M. infestans-infected seed was added, and in the dark, at 30 ° C. The weathering device was immersed for 48 hours to treat the seeds as a medicament. The seeds infected with rice blast fungus were similarly immersed in water and used as a control (no treatment seeds). (3) Cultivating the test plants The seeds were infected with the rice blast disease treated with the above immersion treatment, and sown in a plastic seedling box of 100 cm2 filled with the culture for rice seedlings. Seeding agent The area where the seed is treated is used as the drug treatment area, and the area where the seed is not treated is -17-(15)1379642 as the untreated area. The drug treatment area and the no treatment area were repeated three times. After the soil is covered with the above-mentioned rice seedling culture soil, it is cultured and managed according to the method in a glass greenhouse. (4) Investigating the control effect After 2 weeks of sowing, the rice seedlings of rice blast lesions were found on the leaf surface as the seedlings, and the average number of seedlings repeated three times in each treatment area was used, based on the above (Formula 1), The seedling rate is calculated by the above-described (Formula 2), and the control price of the drug treatment zone is calculated as the control effect. (5) Results The results of the survey are shown in Table 2. As shown in the second table, the disease control agent of the present invention shows a markedly high control effect against rice fever. Table 2 Treatment area Dilution multiple Rice fever disease incidence rate Control price No treatment area _ 7.9% - Preparation example 1 treatment area 200 0% 100.0

<Example 3 > (Rice Rice Bacterial Bacterial Control Test) (1) Preparation of infected seeds Seeds of rice (variety: Fangyi female) were put into a solution containing rice bacterial bacterium, and under decompression conditions, forcibly Inoculated in seeds. wind

CS 18- (16) (16) 1379642 After drying, this seed is used as a seed for bacterial infection of rice blast. (2) Treatment of the drug The preparation prepared by the above Preparation Example 1 was diluted 200 times (mass), and in 15 ml of this solution, 5 g of the bacterial strain of rice bacterial blight was added, in the dark, at 30 ° C. The artificial weatherer was immersed for 48 hours to treat the seeds as a medicament. Seeds infected with rice bacterial blight were similarly immersed in water as a control (no treatment seeds). (3) Incubation of test plants The seeds were inoculated with the above-mentioned immersed rice bacterial bacterium, and seeded in a 100 cm2 plastic seedling box filled with the rice seedling culture. The area where the seed treatment treatment seed is used as the drug treatment area, and the area where the seed is not treated is seeded as the no treatment area. The drug treatment area and the no treatment area were repeated three times. After the soil is covered with the above-mentioned rice seedling culture soil, it is cultivated in accordance with the standard culture in a glass greenhouse. (4) Investigation of the control effect After 2 weeks of sowing, it was found that the rice seedlings of the rice blast fungus, such as spoilage, wilting, and bleaching, were used as seedlings, and the average number of seedlings was repeated three times in each treatment area. Based on the above (Formula 1), the seedling rate was calculated. Then, the seedling rate is calculated from this, and based on the above (Formula 2), the price of the drug treatment zone is determined as a control effect.

C S -19- (17) 1379642 (5 ) Results The results of the survey are shown in Table 3. As shown in the third table, the disease control agent of the present invention shows a markedly high control effect against rice bacterial blight. Table 3 Treatment area Dilution multiple Rice blast bacterial disease rate Control price No treatment area - 27.5% Preparation example 1 Treatment area 200 2.3% 91.6

<Example 4 > (Testing for control of rice seedling bacterial disease) (1) Preparing seeds of rice (variety: Fangyi female) into a solution containing the bacterial pathogen of the seedlings under reduced pressure, Forced to inoculate seeds. After air drying, the seed is infected as a seed of the bacterial pathogen. (2) The drug treatment was diluted with water to a ratio of 200 times (mass) of the preparation prepared in the above Preparation Example 1, and in 5 ml of this solution, 5 g of the seedlings infected with the bacterial bacteria at 15 ° C for 5 days were added. In the dark, it was immersed in an artificial weather device at 3 °C for 24 hours to treat the seeds as a medicament. Seeds infected with Bacillus subtilis were similarly immersed in water as a control (no treated seeds). -20- • (18) 1379642 * (3) Incubation of test plants 'The seeds were infected with the above-mentioned immersed seedlings, and seeded in a plastic seedling box of 100 cm2 filled with rice seedling culture. The area where the seed treatment treatment seed is used as the drug treatment area, and the area where the seed is not treated is seeded as the no treatment area. The drug treatment area and the no treatment area were repeated three times. After the soil is covered with the above-mentioned rice seedling culture soil, it is cultivated in accordance with the standard culture in a glass greenhouse. (4) Investigation and control effect After 2 weeks of sowing, it was found that the seedlings of the seedlings with bacterial diseases such as spoilage, wilting and whitening of rice seedlings were used as seedlings, and the average number of seedlings repeated three times in each treatment area was used.値, based on the above (Formula 1), the seedling rate was calculated. Then, the seedling rate is calculated from this, and based on the above (Formula 2), the price of the drug treatment zone is determined as a control effect. • (5) Results The results of the survey are shown in Table 4. As shown in the fourth table, the disease control agent of the present invention has a markedly high prevention effect on rice seedling bacterial disease. Table 4 Treatment area Dilution multiple Seedling rate of seedling bacterial disease Control price No treatment area - 100.0% _ Preparation example 1 Treatment area 200 8.3% 91.7

CS -21 - (19) (19) 1379642 <Example 5 > (rice brown strip disease control test) (1) Preparation of infected seeds Seeds of rice (variety: Fangyi female) were put into a solution containing brown fungus Forcibly inoculated into the seeds under reduced pressure. After air drying, the seed is infected as a seed of brown truffle. (2) The drug treatment was diluted with water to a ratio of 200 times (mass) of the preparation prepared in the above Preparation Example 1, and in 5 ml of this solution, 5 g of the seeds were infected with the brown fungus which was allowed to absorb water at 15 ° C for 5 days. In the dark, it was immersed in an artificial weather device at 30 ° C for 24 hours to treat the seeds as a medicament. The seeds infected with Rhizoctonia solani were similarly immersed in water as a control (no treatment seeds). (3) The test plants were incubated with the immersed brown truffles and sown in a rice cultivating product. 〇cm2 plastic seedling box. Seeding agent The area where the seed is treated is used as a drug treatment area, and the area where no seed is treated is seeded as a no-treatment area. The drug treatment area and the no treatment area were repeated three times. After the soil is covered with the above-mentioned rice seedling culture soil, it is cultured and managed according to the method in a glass greenhouse. (4) Investigation and control effect

< S -22- (20) (20) Ϊ3?9642 After 2 weeks of sowing, rice seedlings of brown streak disease such as spoilage, wilting and browning of rice seedlings were found as seedlings, and each treatment area was repeated. The average 値 of the number of seedlings of the three times was calculated based on the above (Formula 1). Then, the seedling rate was calculated, and based on the above (Formula 2), the price of the drug treatment zone was calculated as the control effect. (5) Results The results of the survey are shown in Table 5. As shown in Table 5, the disease control agent of the present invention shows a markedly high control effect on rice brown streak disease. Table 5 Treatment area dilution multiple disease incidence seedling rate control price without treatment area _ 1.9% _ Formulation Example 1 treatment Region 200 0.3% 84.2 <Example 6 > (Test for control of rice seedling blight due to bacillus) (1) Inoculation of pathogenic bacteria in culture soil for rice seedlings, uniformly mixed with rice and rice for seedling cultivation The cultured bacillus culture is used as a contaminated soil for rice seedlings caused by sputum. (2) Pharmacy treatment sowing rice (variety: Koshihikari) in a rice seedling box filled with the above-mentioned rice C £ > -23- (21) (21) 1396642 of the seedling blight contaminated soil . Next, a solution of the preparation example 1 in an amount of 1 (by mass) was diluted with water, and uniformly sprayed (25 ml) on the surface of the soil to obtain a drug treatment zone. A seedling box in which the above-mentioned chemical solution was replaced with water was prepared as a control (no treatment zone). The drug treatment area and the no treatment area were repeated twice. (3) Cultivating the test plants The soil for the rice seedlings is covered with the culture soil in the nursery box for carrying out the above treatment, and then cultivated in a glass greenhouse according to the method. (4) Investigation and control effect After 2 weeks of sowing, it was found that the seedlings of the seedlings of the blast fungus, such as spoilage, wilting, browning, etc., were used as seedlings, and each treatment area was repeated twice. The average 値 of the number of seedlings was calculated based on the above (Formula 1), and the seedling rate was calculated. Then, the seedling rate is calculated from this, and based on the above (Formula 2), the control price of the drug treatment zone is calculated as the control effect. (5) Results The results of the survey are shown in Table 6. As shown in the sixth table, the disease control agent of the present invention shows a markedly high control effect against rice seedling blight of sputum. (22) 1379642 The dilution factor of the treatment table of the sixth table is due to the incidence of seedling blight of the mitochondrial disease. The treatment rate is no treatment area _ 20.5% Preparation Example 1 Treatment area 1000 4.7% 77.1 <Example 7 > (Ingen Moldy rice seedling blight control test) (1) Inoculation of pathogenic bacteria in rice culture soil, mixed with root mold culture cultivated with alfalfa and rice seedling culture soil, as rice seedlings of Rhizopus Blight disease contaminates the soil. (2) Pharmacy treatment Seeding rice (variety: Koshihikari) is a plastic seedling box filled with l〇〇cm2 of the above-mentioned rice seedling blight contaminated soil of Rhizopus. Next, a solution of the preparation example 1 in an amount of 1 (by mass) was diluted with water, and uniformly sprayed (25 ml) on the surface of the soil as a drug treatment zone. A seedling box "with water instead of the above-mentioned drug solution" was prepared as a control (no treatment zone). The drug treatment area and the no treatment area were repeated twice. (3) Cultivating the test plants The soil for the rice seedlings is covered with the culture soil in the nursery box for carrying out the above treatment, and then cultivated in a glass greenhouse according to the method. -25- (23)1379642 (4) Investigation of the control effect After 2 weeks of sowing, it was found that rice seedlings, such as spoilage, wilting, root growth retardation, etc. The average 値 of the number of seedlings repeated twice in each treatment zone was calculated based on the above (Formula 1). Then, the seedling rate is calculated from this, and based on the above (Formula 2), the control price of the drug treatment zone is calculated as the control effect. (5) Results The results of the survey are shown in Table 7. As shown in the seventh table, the disease control agent of the present invention shows a markedly high control effect against rice seedling blight of Rhizopus. Table 7 Treatment area Rare number The seedling blight of Rhizopus oryzae Seedling rate Control price No treatment area - 54.1% Preparation example 1 Treatment area 1000 14.9% 72.5

<Example 8 > (Test for control of rice seedling blight by Trichoderma spp.) (1) Cultivation of Trichoderma strain cultured in a culture soil of rice seedlings by inoculation of pathogenic bacteria in culture soil for rice seedling cultivation As a contaminated soil for rice seedlings caused by Trichoderma. -26- - (24) 1379642 ' (2) Pharmacy treatment ‘ Sowing rice (variety: Koshihikari) is a 100cm2 plastic seedling box filled with the above-mentioned rice seedling blight contaminated soil of Trichoderma. Next, a solution of 1000 parts by mass of the preparation example 1 was diluted with water, and uniformly sprayed (25 ml) on the surface of the soil to obtain a drug treatment zone. An incubator in which the above-mentioned chemical solution was replaced with water was prepared as a control (no treatment zone). The drug treatment area and the no treatment area were repeated twice. (3) Cultivating the test plants The rice was cultivated with the culture soil for the rice seedlings, and then cultivated in a glass greenhouse according to the method. (4) Investigating the control effect After 2 weeks of sowing, it was found that the rice seedlings caused by the spoilage of the seedlings, such as spoilage, wilting, and growth retardation of the roots, were used as seedlings, and each φ treatment area was repeated 2 The average 値 of the number of the next seedlings was calculated based on the above (Formula 1). Then, the seedling rate is calculated from this, and based on the above (Formula 2), the control price of the drug treatment zone is calculated as the control effect. (5) Results The results of the survey are shown in Table 8. As shown in the eighth table, the disease control agent of the present invention shows a markedly high control effect against rice seedling blight of Trichoderma. -27- (25) (25) 1379642

Table 8 Dilution multiple of treatment area due to Trichoderma spp. Seedling rate Incidence rate No treatment area _ 26.7% - Preparation Example 1 Treatment area 1000 12.6% 52.8 Industrial Applicability According to the present invention, the following characteristics can be provided Microbial preparation. • Highly effective effects on various diseases caused during rice seedlings 〇 • Safe and less impact on the environment. • There are no or very few restrictions on the number of uses compared to general chemically synthesized pesticides. -28

Claims (1)

1379642 1st and 1st revision of this I Patent Application No. 095,126,974 Patent Application Revision of the Chinese Patent Application No. </ RTI> </ RTI> </ RTI> </ RTI> </ RTI> </ RTI> </ RTI> </ RTI> </ RTI> </ RTI> </ RTI> </ RTI> A filamentous fungus belonging to the genus Talaromyces Y-9401 (BCRC 930085) having an antagonistic effect 2. A controlling agent for diseases caused by rice seedling raising period in the first application of the patent scope, wherein rice seedling raising period The pathogenic bacteria causing the disease are rice seed infectious pathogens or soil infectious pathogens. 3 · The control agent for the disease caused by the rice seedling period in the second paragraph of the patent application, in which the pathogen causing the disease during the rice seedling period is Gibberella fujikuroi; the rice heat pathogen ( Pyricularia oryzae); the rice flax leaf Pathogen (Cochliobolus miyabeanus); Pseudomonas glumae; Pseudomonas plantarii; Pseudomonas avenae; Fusarium genus of rice seedling blight At least one of Pythium, Rhizopus, and Trichoderma. 4. A method for controlling a disease caused by a rice seedling raising period, which comprises treating a rice seed, a rice seedling, a seedling soil or the like by a controlling agent for a disease according to any one of claims 1 to 3; The step of seedling culture medium.