TWI362267B - - Google Patents

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1 · 1362267 DECEMBER 213⁄4 IX. DESCRIPTION OF THE INVENTION: TECHNICAL FIELD OF THE INVENTION The present invention relates to a pharmaceutical composition or food for the manufacture of a pharmaceutical composition or food for regulating blood sugar and improving insulin release. use. [Prior Art] ^ According to the statistics of the Department of Health of the Executive Yuan, the number of people with diabetes has grown at an alarming rate every year, making diabetes the top ten causes of death among Chinese people. In addition, φ, diabetes and cerebrovascular diseases among the top ten causes of death, hypertension, and heart disease often accompany the disease, which makes it difficult to control. This is a very heavy burden and loss for the individual 'family, society and even the country. Therefore, the regulation of sugar and urine disease is an important issue that cannot be delayed. Clinically, diabetes is divided into two categories: the first type of "Tengdaosu-dependent" diabetes and the second type of "non-Tengdao-dependent" diabetes. The main reason for the formation of type 1 diabetes may be that the individual's genetic inheritance, viral infection or toxic substances destroy the beta cells of the pancreas' or autoimmune to form anti-p-cell antibody, and cellular immunity attacks the beta cells, causing pancreas Dirty can not normally secrete insulin, therefore, the reason for the injection of insulin to treat e and the formation of type 2 sugar urinary disease is still unclear; speculation may be related to family heredity or personal eating habits. In addition, such patients are often associated with the emergence of insul in resistance; mainly due to the excessive secretion of insulin (hyperinsulinamia) to form insulin resistance, resulting in bone path muscle, adipose tissue and liver and other peripheral tissue pairs Insulin sensitivity reduction reduces the use of glucose by tissues and causes hyperglycemia. Controlling blood sugar, moderate exercise and balance 1362267 ~ December 2, 2006 Diet is the three principles for the treatment of diabetes. The currently used oral hypoglycemic drugs are two types of sulfourea (Sulfonylurea) and biguanide (Biguanides; BG), which are combined with insulin injection to control the blood sugar of people. Drugs that are considered to be based on urea, such as tolbutamide, glibizide (Glibenese), glibenclamide (Euglucon), etc., are effective in non-inhibition by stimulating insulin secretion in the body and enhancing the binding of insulin to the receptor. Insulin is controlled by patients with diabetes mellitus and is not effective for diabetes that cannot secrete insulin. In addition, biguanide drugs are mainly to improve glucose utilization, but do not stimulate insulin secretion, such as metf〇rmin. Recently, clinical attempts have also been made to use oral hypoglycemic drugs such as thiaz〇lininedi〇nes which enhance insulin sensitivity. However, the injection of yoghurt or oral hypoglycemic agents, although effective in controlling blood sugar, can not effectively inhibit the heart, blood vessels, eyes, kidneys and nervous system caused by diabetes patients, so in order to make patients more The choice of multiple medications, & more effective control of gray sugar to prevent the occurrence of complications, it is urgent to talk about the occurrence of chromium to produce different drugs or healthy foods to assist the use of Sa bed. SUMMARY OF THE INVENTION The present invention relates to a food and pharmaceutical composition for regulating blood sugar. "(4) The purpose of the invention is to use the mixture containing the honey-salted fruit mixture for the purpose of blood sugar regulation, a 4L, m Θ you provide - although the 'la sacred fruit mixture is used to manufacture blood sugar regulation and improve the release of insulin Pharmaceutical composition or 6 1362267 • · ~w^December 2nd; the use of food, wherein the honey fruit mixture comprises a therapeutically effective amount of honey fruit extract and human powder. The invention is also related to A pharmaceutical composition for regulating blood glucose, comprising: a therapeutically effective amount of a fruit extract of honey fruit. Preferably, the therapeutically effective amount of fruit extract of honey fruit is at least 40 mg/kg body weight. The therapeutically effective amount of the honey fruit extract is from 4 mg/kg body weight to 12 mg/kg body weight. Preferably, the pharmaceutical composition maintains a hypoglycemic effect for at least two hours. Preferably, the pharmaceutical composition further comprises a human wall. Preferably, the pharmaceutical composition further comprises one or more pharmaceutically acceptable carriers, diluents or excipients. Preferably, the pharmaceutical composition is For the first type Diabetes.

Preferably, the t drug composition is for type 2 diabetes. Preferably, the pharmaceutical composition is capable of improving the resistance to temsin. The invention further relates to a pharmaceutical composition for enhancing the release of muslin which comprises a therapeutically effective amount of a fruit extract of melissa fruit. Preferably, 4 〇mg/kg body weight / therapeutic effective a; the honey fruit extract is at least preferably, the therapeutically effective amount of the honey fruit extract in the rm is 40 mg. /kg Weight to 120mg/kg body weight. The invention further relates to a food product which is tunable and dexterously controlled to contain blood sugar, which comprises a fruit extract of honey fruit. 7 1362267

Correction and Replacement on December 2, 100 [Embodiment] The present invention relates to a food and pharmaceutical composition for regulating blood sugar, which is a fruit extract containing honey fruit. The poor fruit is the mountain ritual, the scientific name

Synsepalum dulcuficum {Schum.) Daniel 1, Sap〇tacfea (also known as miracle fruit, mysterious fruit). Mela St. Fruit is a kind of fruit that can transform acidic fruit into a succulent fruit. It is native to the western tropical jungle evergreen shrub. The safflower fruit is about two to six meters high, densely clumpy, and the honey sacred fruit is clustered at the end of the branch. It is dark green, with short petiole, broadly lanceolate or oblanceolate, with round to short tips. Shape, about eight pairs of veins, the fruit is a berry, when it is mature, it is dark a red Z to 4 years to bloom. Honey fruit is rich in glycoprotein and high in antioxidants: vitamin C, vitamin KI, citric acid, malic acid, 'succinic acid, etc. The seeds contain natural sterols and the like. Single trace mineral element. Qiaozhen specializes in ginseng) to be able to make up for the "small five dirty heart puzzle" ^ The dry root of perennial herbaceous plants of the five-plus family has long been valued in traditional medicine; Chinese medicine - thinks that "Shen Nong's Herbal Classic" lists it as " The top grade, said the spirit of the spirit, the soul of the soul, the convulsions, the evil spirits, the eyesight: the door has a big vitality, spleen and qi, health: efficacy; clinical application is very broad, and the benefits are also Commonly used for thirst is t$丨之-. In contemporary medicine, it is also a. 曰β ^ used prescriptions for human sputum research. At present, it is active and ginsenoside, which is the main active ingredient of human beings. Human oyster soap 8 1362267 Anti-aging anti-aging, anti-infection 'Inhibition of cancer cell growth, blood fat, fat absorption, strengthening, strengthening immunity 'Qian Jing 珙 static pain, lowering blood sugar, protecting the heart muscle, improving blood coagulation and other effects.

Mela Shengguo can transform the original acidic fruit into a sweet taste by changing (4). The present invention utilizes Melaleus Extract as a food additive for diet control, and provides a good food supplement for diabetic patients with long-term f-dose control. (4) The f (four) fruit extract of the present invention is suitable for human nutrition, and may be beneficial to assisting sugar agitation. Further, the present invention utilizes Mela Extract as a pharmaceutical composition for the purpose of blood sugar regulation. In a preferred embodiment of the present invention, the honeydew fruit extract and the human cockroach are extracted with a mixture of mango and fruit extracts: a mixture ratio of human cockroaches = 1:9, and a mixture of melamine and fruit is prepared. Meila_ginseng mixt (10)) is used as the following examples of the invention. : Example 1: Preliminary test of the regulation of blood sugar by the mixture of honey and fruit. 1. Experimental animal source Wlstar male rats were taken from the National University of Success School Medical School, and the ages were all over 8 weeks' weight at 200 i 25 Between the grams. The rats used were kept in the heart, the air conditioner was maintained at room temperature at 25 ± lt, and the light time and dark time were each 12 hours. The rats were free to eat and drink. 1.2 Induction of insulin-dependent (type 1) diabetic rats According to the usual method, the rats of the above-mentioned strains of 8 weeks old were fasted, intraperitoneally injected with pentobarbital 30 mg/kg, and 65 mg/kg from the femoral vein. Streptozotocin (STZ); STZ was dissolved in ρΗ4.5 physiological saline containing sodium citrate (1 Ommo 1 /L). One week after the injection, blood was collected from the tail vein of anesthetized rats according to the above method according to 9 1362267 for measuring fasting glucose. When the fasting blood glucose of rats was greater than 3_g/dL, there was diabetes thinning and more symptoms. , drink more, more urine), that is, the first type or 姨-dependent diabetic (IDDM) rats, referred to as "milk-induced diabetic rats U non-insulin-dependent (type 2) diabetic rats induction Male rats of the above-mentioned strain 8 weeks old were fed with high amounts of fructose

(60%) of the feed (purchased from Teklad, Medis〇n, we), let the rats eat freely. After three weeks of feeding, A mice developed a phenomenon similar to type 2 diabetes. The insulin-resisting drug, tolbutamide ('t〇lbUtamide), which stimulated the pancreas, was used to determine that the rats had developed insulin resistance. If toluene butyrate (t〇lbutamide) is significantly attenuated or disappeared in rats, it is considered to be a non-insulin-dependent type (NIDDM) diabetic rat, that is, a type 2 diabetic rat. 1 · 4 administration method is a mixture of honey and fruit, after oral administration for a few minutes, the blood sugar of the rats can be lowered, so it will be low dose. According to the health food safety and initial evaluation, the dose of 40 mg/kg 9〇40 mg/kg is regarded as the commonly used dose evaluation method, in order to prove the relationship between dose and action, compared with 3 dose groups, ie 4〇mg/kg (low dose) ), _g/kg (medium dose) and 8〇_ at high dose). In the initial assessment, rats who were fasting for one night were given anesthesia by intraperitoneal injection of 30 mg/kg pentobarbital after ingestion of honeydew fruit mixture and were in Q minutes, 6G minutes, 9Q minutes respectively. And 12 minutes of December, every 2 points of the positive replacement, the tail vein blood was taken, and the blood glucose concentration or the sulphate content was measured. The control group (8 animals) was the same amount of physiological saline. In the assessment of long-term effects, the animals were fed a mixture of honey and fruit for two times a day. After two consecutive doses of blood, the blood sugar level before the feeding was used as a reference (the third day), and then the knife was separately J collected blood on the 2nd day, 5th day, the 10th day and the 15th day after the start of feeding, and measured the blood sugar level at the same time point (and 9G minutes of the third irrigation day). Evaluate the long-term effects of the Miragrass mixture. Four consecutive days of daily administration for fourteen days, observing the long-term effect of the administration of honey salad on the blood sugar of normal rats. The results showed that the same hypoglycemic effect still appeared in the long-term mixture of fruit. For example, given the high dose (80mg/kg) group, please refer to the first and the first table, the hypoglycemic ratio is 24 2 from the original first day.

Heart soil 1·6/0, 21. 5U 2.1% on the fifteenth day, there is no significant difference between the two, °~

-··&quot;The staff does not give a mixture of honey and fruit for a long time. For normal rats, the body does not change, and there is a phenomenon that it is impossible to continue hypoglycemia. Among them, the figure "p<lt;0.001" is shown to have a significant difference, that is, in Table 1, long-term feeding of "Honey 4, Li. 3 into &amp; sugar. [Fruit, one thing" is normal White rat also 11 1362267

Solvent (physiological saline) 0·6±2·1 -0·7±2.2 2.8±3.4 September 14, revised replacement Date 5 Date 10 DatP 15 meila-ginseng mixture 40 mg/ Kg 60 mg/kg 80 12.3±2.1*** 10.3±1.8*** 10.7±3.6*** 11.7±3.0*** 11 9±3 7*** 18.1 Soil 2.1*** 16.7±1.8*** 17.3±1.5*** 17.8±0·9*** 16 1±2 4*** 24.2±1.6*** 21Λ±2Λ*** 19.0±1.9*** 19.0±2.7*** 21.5±2.1 * ** 1 · 5 blood glucose and blood insulin detection methods and effects of blood obtained from the vein, centrifuged to obtain serum, using glucose reaction reagent to detect changes in blood sugar. The principle of the glucose reaction reagent is the glucose oxidase method: after being catalyzed by glucose oxidase, glucose is oxidized to glucuronic acid and cerium peroxide, and is oxidized by a glucose dyeing machine to exhibit a deep red color. The blood glucose level is a red color change in response to the reaction of plasma and glucose reagents, and the blood glucose level is converted from the difference in absorbance to the standard. The blood insulin content was measured by a commercial reagent (purchased from Penisula Lab., Inc., USA), and the change in blood insulin concentration was measured by the ELISA principle. 1. 6 Rat body weight, food intake and drinking water record daily weight of rats (g) 'food intake (g / day) and drinking water (g / day); food intake and drinking water is the comparison of feed weight or The difference in drinking water is different - the difference in days is the weight of the day before the end of the experiment from the day before the end of the experiment. U glucose challenge test (gluc〇se challenge test) Take normal overnight Wi star rats, and the rats were divided into the control group and the three groups of experimental groups by the above method. Group of eight animals. The control group was fed the same amount of physiological saline, and the experimental group was given a dose of 40, 60 and 80 mg/kg of the honeydew fruit mixture by gastric tube administration. After 60 minutes, the rats were tested by intraperitoneal injection of 0.5 g/kg of glucose, which was zero (0). The blood after 5 '10, 20, 30, 60, 90, and 120 minutes was collected according to the usual method, and the change in blood glucose level was measured.

Clinically, glucose challenge trials are often used to assess tissue utilization of sugar. In the glucose challenge trial, it was found that the Miragrass mixture did increase the tissue's utilization of sugar. Taking high-dose (80mg/kg) rats as an example, the blood glucose changes only rose to 192. 4 soil 3. 8mg/dL within five minutes after the injection of sugar, and the control group (the same amount of physiological saline) In comparison, the rise in blood glucose (250. 7 ± 3. Omg / dL) was significantly smaller. In addition, please refer to the second and second tables. The mixture of honey and fruit extract can quickly digest extraneous hyperglycemia, and the hypoglycemic effect can be maintained for two hours. It shows that the honey salad mixture has increased. The efficacy of tissue sugar utilization. The "***" shown in the figure is significantly different, P < 0.001. v Table 2. Effects of a mixture of honey and fruit extracts on the glucose challenge test in normal rats. _ 0 minutes 5 minutes 10 minutes 20 minutes 30 minutes 60 minutes 90 minutes, n solvent (physiological saline 99.8 ± 3.0 250.7 ± 3. 0 201 · 7 ± 3 · 3 149.6 ± 5.8 124.8 ± 4.1 115.4 ± 4.0 106.4 ± 4' 2 95.8±3.9 ) Mela St. Fruit Mix 40mg/kg 97.5±4.7 98.8±5.6 98.4±4.3 220.1±2.8 180.1±4.5 109.6±5.5 98.6±2.7* %t^ 1 / / · -i- ♦ Pingzhongzhong 60 mg/kg 97.2±4.4 210.9±1.8 177.3±4.1 104.0±2.1 97.8±2.6* 1 Ο · 1 J win 33⁄43⁄4 95.7±3.5 92.7±3.1 13 1362267 September 14 hundred positive replacement 80mg/kg 104.4 soil 3.4 192.4±3.7 166.7+6.2 122.3±6.3 1〇5.4±4.2 94.7±2.5* 100.0±3.7 102.5±1.8 Wood P &lt; 0.05, ** Ρ&lt;〇·(Η,木** Ρ&lt;〇.〇〇1 with Differences in data obtained at the same time point in the control group. 1 · 8 intraperitoneal glucose tolerance test (IPGTT) • Glucose-insulin in index as an evaluation of the mixture of honey and fruit Effect of insulin resistance in mice. Evaluation process # as follows: daily feeding of 40 mg/kg, 60 mg/kg or 80 mg/kg of Miragrass mixture to the first Type 2 diabetic rats, 8 animals per group, twice a day for 2 weeks. The diabetic rats in the control group (n=8) were fed the same amount of physiological saline according to the same method. On the last day of the experiment, The diabetic rats used for evaluation were free to eat for one day. Then, one day after fasting, the glucose tolerance test was conducted. The glucose tolerance test procedure was as follows: intraperitoneal injection of pentobarbital at 3 mg/kg in rats. Anesthesia, with zero (0). In the intraperitoneal injection of lg / KG glucose to test the old sputum, ί and then a common method to collect 3 〇, 6 〇, 9 〇 and 12 〇 minutes of blood, measured at different times The lower blood glucose level (mg/dL) and the insulin concentration change. Finally, the glucose and insulin concentration changes measured by the glucose tolerance test are converted into the area under the time-concentration curve (area under curve, AUC). The conversion was glucose-insulin index. Finally, the effect of the honey-salt mixture on the resistance of Tengumycin was evaluated by the change of glucose-Tengdao index. The smaller the value of the indicator, the better the insulin working ability (the better the sensitivity of the membrane); conversely, the larger the index value, the worse the work ability of the insulin (the worse the insulin sensitivity). 14 Γ362267 [Revision and replacement on September 14, 100. 1. 9 results of statistical analysis experiments, all expressed as "mean ± standard error (mean ± SE)" • The percentage of blood glucose lowering is given to the mixture of honey fruit The resulting 'rat blood glucose value' was deducted from the blood glucose obtained after administration of the same amount of vehicle (physiological saline). The difference between the two was divided by the blood glucose level of the rats obtained after the same amount of vehicle was given, expressed as a percentage (%). At the same time, the difference between the two was evaluated by the AN0VA analysis using the general biometric method; P&lt;〇. 05, which was considered to be significantly different. 'Example 2: Regulatory test for normal and diabetic rats 2 _ 1 Mela sacred fruit mixture for normal rat regulation test will be fasted overnight rats, oral administration of different doses of honey salad mixture ' They were 40, 60, and 80 mg/kg, respectively, and then anesthetized with pentobarbital. Please refer to the third and third tables. After 60 minutes, it was found that the 4〇mg/kg dose of the Miragrass mixture can produce hypoglycemic effect in normal rats. The blood glucose level was originally 1〇8 4± _ 2. 0mg / dL decreased to 98. 7 ± 2. 9mg / dL, the percentage of hypoglycemia reached 9. 〇 ± 2. 3% - blood glucose - and decreased in the dose-dependent manner. In addition, the hypoglycemic effect of the Miragrass mixture is increased by 4 times. After 9 minutes of feeding, the effect will reach the maximum. Table 3, the mixture of the honey-salted fruit mixture is produced for normal rats. The effect of lowering the sugar. 15 1362267 December 2, 100 correction replacement 0 minutes 60 minutes 90 minutes 120 minutes solvent (physiological saline) 108.5 ± 2.6 107.6 ± 3.6 107.0 + 2.4 108.0 ± 3.5 Mela fruit mixture 40 mg / kg 60 mg / kg 80 Mg/kg 108.4+2.0 106.0±2.8 104.1±3.9 95.7+3.0* 87.0+1.31 78.3±2.71 85.1+2.61 81.1+2.51 74.0+251 87.9±1.91 83.0+2.11 75.6±3.51 * P&lt;0. 05,2 P< 0. 01, 2 *P<0. 001 The difference between the data obtained at the same time point and the control group. In addition, in order to understand the insulin release effect of the Miragrass mixture on normal rats, the blood insulin concentration was measured at the same time point (ie, 90 minutes after the honey salad mixture). . Referring to the fourth and fourth tables, it was found that the mixture of honey and fruit can also increase the blood insulin concentration in a dose-dependent manner, from the original 172.9±15.5pmol/L to 420.9±15.9pmol/L. It is shown that the honey salad mixture has an effect of promoting insulin release in normal rats. In the figure, "2" indicates p < 0.01, and "1" indicates p < 0.001. Table 4. Effect of the mixture of honey and fruit extract on insulin in normal rats. After 90 minutes of feeding, the vehicle (physiological saline) 172.9115.5 Mela fruit mixture 40 mg/kg 258.6±10.9 60 mg/kg 320.4±14.82 80 mg/kg 420.9 ±15.91 16 1 2. 2 short-term and long-term honey Effect of Lasheng fruit mixture on blood glucose of type 1 diabetic rats 2 P<0.01, 1 P&lt;0. 001 Difference with data obtained from the control group 1362267 _Jg December 2nd revised replacement will be fasting-night Rats, oral administration of different doses of Mola Shengguo mixed mouth knife, 4 40, 60, 80 mg / kg, # pentobarbital (pentobarbitaO 麻 。. Please see the fifth and fifth table oral (10) After the knife knives, the highest dose (80 mg/kg) in the normal rat can produce hypoglycemia. 'In type 1 diabetic rats, the hypoglycemic effect of the honey salad mixture does not occur. So, every day Eat three times, continuously

The effect of the mixture of Mela's fruit mixture on the blood loss of type 1 diabetic rats was evaluated in a fourteen-day manner.

Meila-ginseng mixture 80mg/kg 369.7±8. 376.9±7. 366.0±7. 371.6±8. 362.4±8. ___3 8_6__5_i Please refer to the sixth and sixth tables to show 'long-term administration of honey salad mixture also helps Benefit from controlling blood sugar changes in type 1 diabetic rats. The "*" shown in the figure has a significant difference, that is, p<0.05. Table 6. Effects of long-term feeding of “Mola Fruit Mix” on blood glucose in type 1 diabetic rats induced by st rep tozo toe in 17 1362267 Solvent (physiological saline Saline) I 100 years M~TT5^l Replacement | _ Day 0 Day 2 Day 5 Day 1 Day 15th 365.6±6.8 365.2±7.8 368.4±4.0 365.9±7.4 366 2±5 8 Mela-ginseng mixture 80mg/kg 369.7±8.3 366.9±7.8 356.7±7.6 350.6±8 5 342Λ±^Λ _____*** • Each group of data in the table was obtained from 8 diabetic rats with mean ± SE for blood glucose (unit: mg/dL). Rats fed the same amount of vehicle (vehicle) three times per serving were used as a control group; diabetic rats for 80 mg/kg products were compared with each other. 2.3 The effect of long-term administration of honey salad mixture on blood glucose in type 2 diabetic rats Before the start of the experiment, the second type of diabetic rats were first intraperitoneally injected with 10 mg/kg 〇 续 but urea (t〇ibutamide) to confirm Whether its hypoglycemic effect disappears or weakens due to the formation of resistance to tamsin. Then, the effect of the Miragrass mixture on the blood-reducing effect of the type 1 diabetic rats was evaluated by four consecutive 'four consecutive days' feeding. Please refer to the seventh and seventh tables. 'The long-term administration of the honey fruit mixture can also be dose-dependent. The blood sugar level is lowered. The figure shows "*" as a significant difference, p&lt;0.001 . Table 7. Effect of long-term feeding of honey-salted fruit mixture on blood glucose in type 2 diabetic rats induced by high-volume (60%) fructose feed 18 1362267 Correction of replacement of day 0~~ 2 days ~ ~ 5th day 10th day 15th day solvent (physiological saline Saline) 121.515.6 116.3±4.2 117.9±4.5 121.4+6.1 120.1±3.5 Mela-ginseng mixture 40 mg/kg 60 mg/kg 80 mg/kg 118.3±4.4 116.915.4 120.2+6.3 113.8+4.6 innnj_. . 101.8±5.2 101.9±7.2 109.9±6.3 *** *** 99.0+4.9* 99.7±7.8* 94.6±3.5* 100.5±6.7 101·7±6_8 96·8±6.0* 98·4±5·1* 96.0±6.0* Shuiyu Umbrella Fortunately, each group of data in this table was obtained from 8 diabetic rats. Mean soil SE represents blood stasis value (unit: mg/dL). Rats fed the same amount of vehicle three times a day were used as a control group; diabetic rats for the same feeding of 40 mg/kg, 60 mg/kg and 80 mg/kg products were compared with each other. * P &lt; 0.05, wood * P &lt; 0. 01, * wood * P &lt; 0. 001 The difference between the data obtained at the same time point and the control group. In addition, as shown in Figure 8, the Miragrass mixture may restore the hypoglycemic effect of tolbutamide; after two weeks of feeding high doses of the Miracle mixture (80 mg/kg), The rate of the hypoglycemic was restored from the original 4. 0 ± 5.45 to 22. 9 ± 4. 35. The recovery of this effect is due to the fact that tolbutamide induces insulin release, but its hypoglycemic effect can be restored, resulting in improved insulin resistance. Therefore, when it is used to improve the insulin resistance of type 2 diabetes, the "***" shown in the figure has a significant difference, which is ρ <0.0 0 1 〇 2. 4 honey fruit The effect of the mixture on insulin resistance in rats is to confirm whether the mixture of insulin extracts can improve insulin resistance. In 19 1362267 • » _ September 14 曰 revised replacement of the invention, further take two weeks of feeding honey The area under the curve of the glucose tolerance test in the second type of diabetic rats of the sacred fruit mixture, and the measured changes in blood glucose and blood insulin concentration, is represented by a histogram. The results are shown in the ninth and tenth figures. : In the blood glucose and insulin concentrations compared with Fukang rats (108.5 ± 2.6mg / dL; 172.9 ± 15.5pmol / L), high-quality (60%) fructose-induced type 2 diabetic rats did come high. In the figure, "*" indicates p&lt;0. 001. However, with the addition of the Miragrass mixture, not only does the hyperglycemia state show a dose-related decrease; at the same time, it also reduces the excessive insulin content in the blood. In addition, as shown in Figure 11 and Table 8, the glucose-insulin index commonly used to assess insulin resistance will also decrease as the feeding dose of the Miragrass mixture increases. It is shown that the mixture of honey and fruit extracts can improve insulin resistance. Table 8. The area under the concentration-time curve (AUC) of the glucose-time test conducted by the long-term feeding of the honey-flavored fruit and the blood sugar (glucose) and insulin (insulin) . • &quot; Glucose Insulin Glucose-Insulin Index_______(mg/dLxmin)_(pmol/Lxmin)_(unitxlO6) Solvent 42510.2±145.6 348300.5± 2513.8 157466.0±74.3 Meila-ginseng mixture 40mg/kg 40890.5+100.4*** 265230.61 1543.5*** 108452.0+78.8*** 60 mg/kg 38322.21124.5*** 254220.9±1540.8*** 97422.2±93.5*** 80 mg/kg 37440.6±122.4*** 246960.8 +1613.3*** 92461.1+89.6*** In addition, please refer to the twelfth figure, after giving the first type of agglutination rat honey fruit mixture (80 mg/kg) for three consecutive days, then Insulin is administered intravenously, and the hypoglycemic effect of insulin is enhanced. 20 1362267

It is shown that taking the honey-salted fruit mixture has the effect of enhancing the hypoglycemic effect of the sinensis. It can be seen that the mixture of the sacred fruit has the ability to improve the resistance of the sinensis. The rats are in need of seed, food intake and drinking water. Comparing rat body weight, type 1 diabetic rats were significantly lighter than normal rats, and their body size was also smaller. Table IX. Changes in body weight of type 1 diabetic rats with long-term feeding of honey-salted fruit mixture _ days _ 〇 2nd 5th 1〇~^ 75^~~ physiological salt 228·6 soil 7. 8 228. 8±8· 5 230. 6±9· 2 235. 6±8. 6±8 9 ^ ' Mela fruit mixture 40mg/kg 223.5 + 8. 9 225.5 + 5.8 230. 5±5. 8 234.5 + 7.5 240 5±8 5 60mg/kg 2 21.6±8.2 221.6 + 6. 1 228.6 + 7.7 2 34. 6±8. 8 2 38 6 + 9 8 80mg/kg 228.0 soil 8·9 225. 0±8· 6 228.8±6_ 2 230 ·0±8.4 235: 7Ϊ8: 4 Please refer to Table 10 and Table XI. In terms of food intake and drinking water, the first type of rats eat more than healthy rats, and they show more typical symptoms of diabetes. Feeding the honey fruit mixture does not affect its weight, diet and drink.

The amount of water. Table 10: The number of days of food intake of the first-type rats in the long-term feeding of the honey-salted fruit mixture 0 2nd 5 th 10th 15th physiological saline salt honey fruit 38.6+5.8 38.8+4. 5 40. 6±9. 2 38. 6+3.2 42. 6+5. 0 Mixture 40mg/kg 60mg/kg 80mg/kg 43.5+5.9 45.6±6.2 38.0+8.9 45.5+4.8 37.6±6. 5 35.0+5. 6 44.5±8.9 45.6±8.5 40.8+ 6.2 45. 5±6. 5 44.6+4.8 45. 0±7. 8 45.5+8.3 48. 6+8. 9 7+8. 6 Table XI, long-term feeding of honey fruit mixture for type 1 diabetes Large 21 1362267 Correction of the water intake of the white rats on the 14th of the 100th day of the year 0 2nd 5th 10th 15th Solvent 108.615.8 108.8±10_5 120.6±9.2 118.6±8.2 112.6±11.9 Mela-ginseng mixture 40 mg/kg 60 mg/kg 80 mg/kg 113.5±6.9 121.6±8.2 118.0+10.9 115.5±10.8 117.6±7.1 115.0+8.6 114.5+8.9 115.6±8.7 115.8±9.2 111.5+9.5 111.6±10.8 119.0+8.4 115.5± 8.5 118.6±8.8 126.7111.4 The parent data in the table was obtained from 8 rats, and the drinking water weight (unit: g/day) was expressed by mean soil SE. Rats fed the same amount of vehicle were used as a control group; rats were fed a mixture of 40 mg/kg, 60 mg/kg and 80 mg/kg products. In addition, in terms of weight, type 2 diabetes is significantly fatter than normal rats. However, the filling of the honey fruit mixture does not affect its body weight change. At the same time, there was no significant difference in the amount of food consumed and the amount of water consumed by the animals. It is apparent to those skilled in the art that various modifications and variations can be made without departing from the scope and spirit of the invention. Although the present invention has been described with reference to specific preferred specific embodiments, it should be understood that the invention should not be In fact, the various modifications that are obvious to those skilled in the art are also covered by the following claims. [Simple description of the diagram] The first picture shows the hypoglycemic effect of a mixture of honey and fruit extracts on healthy rats for 14 consecutive days. 22 1362267 Fig. 2 The figure shows the effect of a mixture of honey and fruit mixture on the war test. Jiankang's glucose-picking picture is the effect of a single administration of sugar on the human body. Committed. The lowering of blood in healthy rats The fourth picture is the effect of a single administration of honey. Committed. The fifth panel of normal rat is the effect of hypoglycemic effect in a single dose of honey salad. Dust Diabetes The sixth picture shows the effect of blood glucose on diabetic rats in a mixture of fourteen days. Dust The seventh picture shows the hypoglycemic effect produced by the continuous administration of the honey salad mixture in the second rat. The eighth picture shows the effect of continuous administration of a mixture of honey-salted fruit on tolubutamide (tolbutanude) in hypoglycemic rats in type 2 diabetic rats. The ninth figure shows the change in blood glucose concentration in the glucose tolerance test performed on type 2 diabetic rats after a mixture of honey salads for three consecutive days. The tenth figure shows the change in insulin concentration in the glucose tolerance test performed on type 2 diabetic rats after a mixture of honey salads for three consecutive days. The tenth-graph is the result of the glucose tolerance test performed on the glucose tolerance test in the type 2 diabetic rats after the administration of the f-god mixture for three consecutive days. The twelfth image shows the effect of a mixture of honeysuckle fruit on the hypoglycemic effect of the first type of diabetic rats in three consecutive days. [Explanation of main component symbols] 23 1362267 • Correction replacement on September 14, 100

twenty four

Claims (1)

.^02267 ___ 〇 2 2 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 The effective honey salad mixture contains honey fruit extract and human hair powder extract &amp; 铷, 八参粉, in which honey salad fruit is mixed with human powder 1 : 9. 2. If the patent application scope 丄 composition can be surnamed s τ this medical music ‘received> 'two hours of hypoglycemic effect. The use of any of the items 1 to 2 of the invention, wherein the composition further comprises one or more medicinal materials, diluents or excipients. Medically acceptable carrier 丄::! = Uses in items 1 to 2, and Le and 5 are used for type 1 diabetes. The use of any of the above-mentioned items of the first aspect of the invention, wherein the medical composition is used for type 2 diabetes. 6 · The pharmaceutical composition of the patent scope can improve insulin resistance n ' ' 7. A therapeutic fruit extract that can regulate blood sugar and a mixture of human scorpion containing honey sage powder For the = powder' wherein the honey fruit extract and the human maintains the same as: the pharmaceutical composition described in item 7 of the profit range, which can be hypoglycemic for one hour. ^ If the patent application range is 7 or 8 The medical device described in the item, further comprises one or more, sigma, medically acceptable carrier, diluent or excipient. 25 1362267 Modified on February, 2011. Replacement 10 Or the pharmaceutical composition according to the invention, which is for use in the first type of diabetes. 11. The pharmaceutical composition according to claim 7 or 8, which is for use in type 2 diabetes. The pharmaceutical composition according to Item 7 or 8, which is capable of improving insulin resistance. XI. Schema: 26