TW202509310A - Composite polyester fiber, polyester fiber cloth, fiber cloth carrier and preparation method and use thereof - Google Patents

Abstract

A composite polyester fiber, and a polyester fiber cloth, a fiber cloth carrier and a cell culture carrier which comprise the composite polyester fiber, and a preparation method and use. When the polyester fiber cloth, the fiber cloth carrier or the cell culture carrier which are prepared from the composite polyester fiber are used for cell culture, the glycometabolism capability of cells can be improved.

Description

Composite polyester fiber, polyester fiber cloth, fiber cloth carrier and preparation method and use thereof

The present invention relates to the technical field of composite polyester fibers, and in particular to a composite polyester fiber, a polyester fiber cloth made of the composite polyester, a fiber cloth carrier, and a preparation method and application thereof.

At present, large-scale animal cell culture technology has been widely used in the production of various cells and cell products, including enzymes, growth factors, vaccines, antibodies and other biological products. Most animal cells have the habit of growing by adhering to the wall, so when they are cultured in vitro, they usually need to provide support for their growth. This support is what technicians in this field often call cell culture carriers. Current cell culture carriers include granular carriers with a diameter of 2-11 mm, porous spherical carriers or sheet fiber carriers. Current microcarriers include fibrous protein and chitosan macroporous microcarriers, chitosan and gelatin mixed microcarriers, etc. Spherical microcarriers have the problem that cells can only attach to the surface and the area-to-volume ratio is small. Although porous microcarriers have gaps that are conducive to cell attachment, their gaps are easily blocked and thus affect oxygen and material exchange. At present, there are few studies on fiber scaffold carriers at home and abroad. The main fiber scaffolds are electrospun filament materials, which are mainly used in tissue engineering in vivo transplantation, while there are few purely used for large-scale in vitro cell culture. The structural characteristics of electrospun filament fiber scaffolds are that electrospun filaments are nano-scale microstructures with many gaps, which can simply imitate the distribution of extracellular matrix fibers for cell attachment and growth. However, the gaps are small, and cells are very likely to block the relevant gaps after attaching in sheets, which severely limits the exchange of substances. In addition, they are easy to float on the surface of the culture medium, which is not conducive to the contact between cells and the culture medium.

The smallest component unit of the carrier for cell culture is fiber. The fiber can be polyester fiber in particular.

Polyester fiber is a product that uses polyester in the form of fibers. The most commonly used polyester material is polyethylene terephthalate (PET), and other polyesters such as polybutylene terephthalate and polylactic acid are also used. The methods for preparing polyester into fibers can be roughly divided into melt spinning and solution spinning. Solution spinning is divided into dry spinning and wet spinning depending on whether the solvent used is a volatile solvent. Melt spinning can be simply divided into direct spinning, mixed spinning and composite spinning, among which composite spinning can obtain composite fibers.

Composite fiber refers to chemical fiber made of two or more polymers, or the same polymer with different properties, through composite spinning. According to the different distribution of the two types of polymers on the cross section, it can be divided into skin-core type, segmented type, island type and parallel type.

However, the prior art lacks polyester fibers, polyester fiber cloths, and fiber cloth carriers for efficient cell culture, and lacks methods for obtaining the polyester fibers and preparing polyester fiber cloths/fiber cloth carriers to achieve efficient cell culture.

One of the purposes of the present invention is to overcome the problem of low cell survival rate of cell culture carriers in the prior art when used for cell culture, and to provide a composite polyester fiber with good coarse and fine uniformity, a polyester fiber cloth made from the composite polyester, a fiber cloth carrier, and a preparation method and application thereof. The composite polyester fiber of the present invention, the fiber cloth and the carrier prepared therefrom can significantly improve the cell survival rate and increase the cell reproduction rate when used for cell culture.

The inventors found in the course of their research that by controlling the average diameter D of the composite polyester fiber, the diameter standard deviation of the composite polyester fiber, the dosage ratio of the core layer and the skin layer material, the melting point difference of the core layer and the skin layer material, the intrinsic viscosity difference and the standard deviation, and by using a high-precision spinneret, the dosage ratio of the core layer and the skin layer material, the average diameter D of the fiber and the diameter standard deviation are preferably made to meet the conditions of the present invention, and a composite polyester fiber with good coarse and fine uniformity can be obtained, which can be used to manufacture polyester fiber cloth and fiber cloth carrier for cell culture.

Therefore, the first aspect of the present invention provides a composite polyester fiber having a sheath-core structure; wherein the mass ratio of the core layer material to the sheath layer material is x, and 1.5≤x≤4, preferably 2≤x≤3.6, and more preferably 2≤x≤3; and wherein the average diameter D of the composite polyester fiber is 10-40 μm, preferably 17-32 μm, and more preferably 18-30 μm, and the diameter standard deviation is ≤2.2 μm, preferably ≤2.0 μm, and more preferably ≤1.8 μm.

The second aspect of the present invention provides a method for preparing the above-mentioned composite polyester fiber, which comprises: melting the core layer material and the skin layer material separately to obtain a melt, and spinning the melt to obtain the composite polyester fiber.

The third aspect of the present invention provides a polyester fiber cloth, which comprises the composite polyester fiber or the composite polyester fiber prepared by the above method; for example, the polyester fiber cloth can be prepared by using the composite polyester fiber.

The fourth aspect of the present invention provides a method for preparing the above-mentioned polyester fiber cloth, which comprises laying out the composite polyester fiber and hot pressing to obtain the polyester fiber cloth.

A fifth aspect of the present invention provides a fiber cloth carrier for cell culture, wherein the fiber cloth carrier is made of the above-mentioned polyester fiber cloth.

A sixth aspect of the present invention provides a method for preparing the above-mentioned fiber cloth carrier, wherein the method comprises: pre-treating and/or sterilizing the above-mentioned polyester fiber cloth.

The seventh aspect of the present invention provides the use of the composite polyester fiber in preparing a fiber cloth carrier for cell culture.

An eighth aspect of the present invention provides an application of the polyester fiber cloth or fiber cloth carrier in cell culture.

The ninth aspect of the present invention provides a method for culturing cells, which comprises: sterilizing the polyester fiber cloth and placing it in a culture solution as a cell carrier, or placing the fiber cloth carrier in a culture solution as a cell carrier, and inoculating cells on the carrier for in vitro culture; preferably, the cells are adherent cells, more preferably Vero cells and/or L929 cells.

Through the above technical solution, the present invention achieves the following beneficial effects:

The composite polyester fiber of the present invention has a small diameter and a small standard deviation of the diameter; the composite polyester fiber of the present invention and the polyester fiber cloth or fiber cloth carrier made of the composite polyester fiber or the polyester fiber cloth carrier can significantly improve the sugar metabolism ability of cells when used for cell culture; the higher the sugar metabolism ability, the higher the cell reproduction rate and survival rate (only living cells can metabolize sugar, and dead cells cannot metabolize sugar), that is, the composite polyester fiber and the fiber cloth and carrier made of the composite polyester fiber can achieve better results when used for cell culture. Therefore, the present invention can significantly improve the survival rate of cells and the cell reproduction speed is fast.

The endpoints and any values of the ranges disclosed herein are not limited to the exact ranges or values, and these ranges or values should be understood to include values close to these ranges or values. For numerical ranges, the endpoint values of each range, the endpoint values of each range and the individual point values, and the individual point values can be combined with each other to obtain one or more new numerical ranges, and these numerical ranges should be considered as specifically disclosed in this article.

The first aspect of the present invention provides a composite polyester fiber having a core-skin structure; wherein the mass ratio of the core layer material to the skin layer material is x, and 1.5≤x≤4, preferably 2≤x≤3.6, and more preferably 2≤x≤3; and wherein the average diameter D of the composite polyester fiber is 10-40 μm, preferably 17-32 μm, and more preferably 18-30 μm, and the diameter standard deviation is ≤2.2 μm, preferably ≤2.0 μm, and more preferably ≤1.8 μm.

In the present invention, preferably, the fiber cloth prepared by using the sheath-core structure composite polyester fiber satisfying the above-mentioned mass ratio, average diameter and diameter standard deviation can improve the cell reproduction rate and survival rate, so that the cell sugar metabolism value is above 15 g/day; and when the average diameter and diameter standard deviation of the polyester fiber deviate from the above range, the cell sugar metabolism value is below 10 g/day.

In the present invention, the core layer material is homopolyethylene terephthalate, copolyethylene terephthalate or a combination thereof, preferably homopolyethylene terephthalate, and the skin layer material is homopolyethylene terephthalate, copolyethylene terephthalate or a combination thereof, preferably copolyethylene terephthalate.

In the present invention, the amount of the core material is higher than that of the skin material, and the mass ratio (weight ratio) of the core material to the skin material can be 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, and the range composed of any two of the above points, preferably 2-3.6, more preferably 2-3. By limiting the weight ratio of the core material to the skin material within the above preferred range, a smaller standard deviation of the diameter of the composite polyester fiber can be further obtained.

In the present invention, the skin-core mass ratio can be determined by the following method: about 10 mg of fiber or fiber cloth sample is dissolved in 50-100% mL of o-chlorophenol, and then measured by liquid chromatography such as high temperature liquid chromatography using a non-polar or weakly polar filler such as C18 (ODS); the mobile phase of the liquid chromatography can use a more polar solvent, such as a mixture of trifluoroacetic acid, phenol and 1,1,2,2-tetrachloroethane (mass ratio 60:40), a mixture of phenol and chloroform (volume ratio 1:1), etc.; two main peaks will appear, namely the skin material peak and the core material peak; the peak area of each peak is integrated, and the ratio represents the mass ratio of the skin material to the core material, that is, the skin-core mass ratio. For example, when copolyester uses cyclohexanedimethanol as a comonomer, the homopolyester peaks first and the copolyester peaks later; when copolyester uses isophthalic acid as a comonomer, the copolyester peaks first and the homopolyester peaks later.

In the present invention, the average diameter of the composite polyester fiber can be 10 μm, 15 μm, 17 μm, 18 μm, 20 μm, 22 μm, 24 μm, 26 μm, 28 μm, 30 μm, 32 μm, 40 μm, and a range consisting of any two of the above points.

The diameter standard deviation of the composite polyester fiber may be 2.2 μm, 2.1 μm, 2.0 μm, 1.9 μm, 1.8 μm, 1.6 μm, 1.4 μm, 1.2 μm, 1 μm, 0.8 μm, 0.6 μm, 0.4 μm, 0.2 μm, 0.1 μm, and a range consisting of any two of the above points, such as 0.1-2.2 μm.

In the present invention, the test method for the diameter standard deviation of the composite polyester fiber is: randomly select 50 composite polyester fibers, randomly cut a small section, take a scanning electron microscope (SEM) photo, and measure the diameter of each fiber from the photo (measure each fiber once), accurate to 0.1 μm, the arithmetic mean of the diameters of the 50 fibers is the average diameter of the composite polyester fiber, and then calculate the standard deviation of these diameter values according to the standard deviation calculation method. The calculation formula of the diameter standard deviation is as follows: ,

Where n is the number of composite polyester fiber samples (i.e. 50), i is the fiber sample number, d _i is the diameter of the fiber numbered i, It is the arithmetic mean of the diameters of 50 fiber samples.

In some embodiments of the present invention, the composite polyester fiber is a composite polyester fiber having a length greater than 150 mm.

According to the present invention, preferably, the intrinsic viscosity of the skin material is 0.01-0.18 dL/g higher than the intrinsic viscosity of the core material, preferably 0.01-0.15 dL/g, and the standard deviation of the intrinsic viscosities of the core material and the skin material is ≤0.02 dL/g, preferably ≤0.015 dL/g.

In the present invention, the test method for the intrinsic viscosity of polyester is the intrinsic viscosity test method described in GBT14190-2017. Among them, the test method for the standard deviation of the intrinsic viscosity is: randomly take 6 samples, test the intrinsic viscosity according to the intrinsic viscosity test method described in GBT14190-2017, and take the arithmetic mean of the tested intrinsic viscosity values as the intrinsic viscosity of the polyester. The smaller the standard deviation of the intrinsic viscosity, the higher the uniformity of the intrinsic viscosity of the same batch of materials. The calculation formula of the standard deviation of the intrinsic viscosity is as follows: ,

Where m is the number of samples (i.e. 6), i is the sample number, η _i is the intrinsic viscosity of the sample numbered i, It is the arithmetic mean of the intrinsic viscosity of 6 samples.

According to the present invention, the standard deviation of the intrinsic viscosity of the core layer material and the skin layer material can be independently 0.02 dL/g, 0.018 dL/g, 0.015 dL/g, 0.01 dL/g, 0.009 dL/g, 0.008 dL/g, 0.007 dL/g, 0.006 dL/g, 0.005 dL/g, 0.004 dL/g, 0.003 dL/g, 0.002 dL/g, 0.001 dL/g, 0.0001 dL/g, and a range consisting of any two of the above points, for example, 0.0001-0.02 dL/g.

According to the present invention, the melting point of the core layer material is more than 20°C higher than the melting point of the skin layer material; for example, 20°C, 22°C, 24°C, 25°C, 30°C, 40°C, 50°C, 60°C, 80°C, 100°C, 110°C, 120°C, 130°C, 140°C, 150°C, 160°C, and a range consisting of any two of the above points, for example, 20-160°C; preferably, the melting point of the core layer material is at least 22°C higher than the melting point of the skin layer material, more preferably at least 24°C higher.

In the present invention, the melting points of the core layer material and the skin layer material are measured by differential scanning calorimetry. Specifically, at a heating rate of 10°C/min, the sample is heated from 20°C to 300°C and the peak temperature of the endothermic peak existing between 100°C and 260°C is taken as the melting point.

According to the present invention, surprisingly, the inventors have further found that when the fiber cloth prepared by using polyester fiber having a mass ratio x of the core layer material to the skin layer material and an average diameter D of the polyester fiber satisfying the following formula (1) is used for cell culture, the sugar metabolism capacity of the cells can be further improved: Formula (1),

The value of the average diameter D of the composite polyester fiber used in formula (1) is in μm. For example, if the average diameter D is 20 μm, the value of D in formula (1) is 20.

More preferably, when the mass ratio of the core material to the skin material of the composite polyester fiber is 2-3 and satisfies formula (1), the sugar metabolism capacity of cells can be further improved when the composite polyester fiber and the polyester fiber cloth or carrier prepared therefrom are used for culturing cells.

According to the present invention, in some embodiments, preferably, the core material is homopolyethylene terephthalate. More preferably, the intrinsic viscosity of the homopolyethylene terephthalate is 0.6-0.8 dL/g, such as 0.6 dL/g, 0.62 dL/g, 0.64 dL/g, 0.66 dL/g, 0.68 dL/g, 0.70 dL/g, 0.72 dL/g, 0.74 dL/g, 0.76 dL/g, 0.78 dL/g, 0.8 dL/g, and a range consisting of any two of the above points, preferably 0.62-0.8 dL/g, and more preferably 0.65-0.7 dL/g, and a melting point above 240°C, such as 240°C, 245°C, 248°C, 250°C, 255°C, 260°C, 265°C, 270°C, 280°C, 290°C, 300°C, 320°C, 350°C, 380°C, and a range consisting of any two of the above points, and more preferably 245-280°C.

According to the present invention, preferably, the standard deviation of the intrinsic viscosity of the homopolyethylene terephthalate is ≤0.015 dL/g, preferably ≤0.01 dL/g, and more preferably ≤0.005 dL/g.

According to the present invention, in some embodiments, preferably, the skin material is copolyethylene terephthalate; more preferably, the intrinsic viscosity of the copolyethylene terephthalate is 0.601-0.9 dL/g, for example, 0.61 dL/g, 0.62 dL/g, 0.64 dL/g, 0.66 dL/g, 0.68 dL/g, 0.70 dL/g, 0.72 dL/g, 0.74 dL/g, 0.76 dL/g, 0.78 dL/g, 0.80 dL/g, 0.82 dL/g, 0.84 dL/g, 0.86 dL/g, 0.88 dL/g, 0.9 dL/g, and the range formed by any two of the above points, preferably 0.61-0.9 dL/g, further preferably 0.61-0.82 dL/g, and the melting point is below 240°C, for example 240°C, 230°C, 235°C, 220°C, 200°C, 190°C, 180°C, 170°C, 160°C, 150°C, 145°C, 140°C, 135°C, 130°C, 120°C, and the range formed by any two of the above points, more preferably 130-240°C.

According to the present invention, preferably, the standard deviation of the intrinsic viscosity of the co-polyethylene terephthalate is ≤0.015 dL/g, preferably ≤0.01 dL/g.

According to the present invention, preferably, the copolyethylene terephthalate includes structural units derived from terephthalic acid, ethylene glycol and comonomer Y or is composed of structural units derived from terephthalic acid, ethylene glycol and comonomer Y, and the content of structural units derived from comonomer Y in the copolyethylene terephthalate can be 0.6-20 mol%, for example, 0.6 mol%, 0.75 mol%, 1 mol%, 1.5 mol%, 3 mol%, 5 mol%, 10 mol%, 15 mol%, 18 mol%, 20 mol%, and a range consisting of any two of the above, preferably 0.6-18 mol%, more preferably 0.7-15 mol%. In the present invention, the content of the structural unit of the copolymerized monomer Y refers to the percentage of the molar amount of the structural unit of the copolymerized monomer Y to the total molar amount of the structural unit of terephthalic acid, ethylene glycol and the copolymerized monomer Y.

According to the present invention, there is no particular limitation on the type of the comonomer Y, as long as the difference in melting point and intrinsic viscosity between the skin layer material and the core layer material and the standard deviation of the intrinsic viscosity can meet the requirements. Preferably, the comonomer Y can be selected from at least one of dicarboxylic acids, diols and tetraols. More preferably, the dicarboxylic acid is a dicarboxylic acid containing a benzene ring, and is further preferably isophthalic acid and/or phthalic acid. More preferably, the diol is a diol having 3 to 20 carbon atoms, and is further preferably at least one of butanediol, hexanediol, cyclohexanedimethanol and pentanediol (e.g., neopentanediol). More preferably, the tetraol is a tetraol having 3 to 20 carbon atoms. In the present invention, the number of carbon atoms in the diol or tetraol can be independently 3, 4, 5, 6, 7, 8, 9, 10, 12, 15 or 20.

In the present invention, the copolymer monomer Y can also be selected from acid monomers in other forms such as anhydrides, acyl chlorides, methanol esters, and ethanol esters, and alcohol monomers in other forms such as ethers, hemiacetals, and acetals.

According to the present invention, preferably, the Sb content in the core material and the sheath material of the polyester fiber is less than 20 ppm. In the present invention, "ppm" refers to the "weight" content.

The second aspect of the present invention provides a method for preparing the above-mentioned composite polyester fiber, which comprises: melting the core layer material and the skin layer material separately to obtain a melt, and spinning the melt to obtain the composite polyester fiber.

In the present invention, the shape of each nozzle hole of the nozzle plate used in the spinning process is two concentric circles, the difference between the maximum diameter and the minimum diameter of all inner circles of the nozzle holes (also called the extreme difference, that is, the diameter of the largest hole minus the diameter of the smallest hole) is ≤2 μm, and the difference between the maximum diameter and the minimum diameter of all outer circles of the nozzle holes (also called the extreme difference, that is, the diameter of the largest hole minus the diameter of the smallest hole) is ≤2 μm. In the present invention, the difference between the maximum diameter and the minimum diameter of all inner circles and the difference between the maximum diameter and the minimum diameter of all outer circles can be independently 0.1 μm, 0.5 μm, 1.5 μm, 2 μm, and a range consisting of any two of the above points, such as 0.1-2 μm.

In the present invention, it can be understood that the core layer material passes through the inner circle of the nozzle hole to form the core layer structure of the composite polyester fiber, and the skin layer material passes through the outer circle of the nozzle hole to form the skin layer structure of the composite polyester fiber, and then after cooling and stretching, a composite polyester fiber with a skin-core structure is formed.

According to the present invention, preferably, the inner diameter of the inner circle of the nozzle hole is 15-25 μm, and the inner diameter of the outer circle is 25-35 μm. In the present invention, the precise hole diameter of the nozzle plate is measured by a profiler. For example, the Contour GT profiler of Bruker can be used.

According to the present invention, preferably, the process of spinning the melt to obtain a composite polyester fiber having a sheath-core structure comprises the following steps:

(1) feeding the melt (core layer material melt and skin layer material melt) into a nozzle plate for spinning to obtain a filamentary melt with a skin-core structure;

(2) cooling the filamentary melt having a skin-core structure to obtain primary fibers; and

(3) The spun fibers are stretched to obtain composite polyester fibers.

According to the present invention, preferably, the core layer material melt is obtained by melting the core layer material at 220-280°C (for example, 220°C, 240°C, 260°C, 280°C, and a range consisting of any two of the above points).

According to the present invention, preferably, the skin material melt is obtained by melting the skin material at 200-280°C (for example, 200°C, 220°C, 240°C, 260°C, 280°C, and a range consisting of any two of the above points).

In the present invention, it can be understood that the temperature of the core layer material melt and the skin layer material melt refers to the temperature of the nozzle plate when they are extruded from the nozzle plate outlet.

According to the present invention, preferably, side blowing is used for cooling, wherein the wind speed of the side blowing can be 1.5-2 m/s, for example, 1.5 m/s, 1.6 m/s, 1.7 m/s, 1.8 m/s, 1.9 m/s, 2 m/s, and a range consisting of any two of the above points, more preferably 1.5-1.8 m/s; and the temperature is 15-30°C, for example, 15°C, 20°C, 25°C, 30°C, and a range consisting of any two of the above points, more preferably 15-20°C.

According to the present invention, preferably, high-speed airflow is used for stretching, wherein the flow rate of the high-speed airflow is 2500-5500 m/min, for example, preferably 3000-5000 m/min. Preferably, the use of high-speed airflow stretching can further preferentially orient the structure of the fiber along the processing direction of the fiber, thereby making the fiber coarseness more uniform on the one hand, and making the mechanical properties of the fiber better on the other hand.

According to the present invention, preferably, the stretching method includes at least one of a round tube stretching method and a slit stretching method.

The round tube stretching method in the present invention refers to a method in which a plurality of (e.g., 2-20) spun fibers are fed into a long round tube and the spun fibers are stretched by using air of a certain velocity in the round tube. In the round tube stretching method, the air velocity can be 2500-5500 m/min, preferably 3000-5000 m/min.

According to the present invention, preferably, the inner diameter of the round tube used in the round tube method is 0.5-5 cm, and the distance between two adjacent round tubes is 0.5-10 cm. The distance between two adjacent round tubes refers to the distance between the centers of the two adjacent round tubes. The length of the round tube can be 0.1-2 m. The length of the round tube refers to the distance along the fiber ejection direction.

According to the present invention, preferably, the spacing of the slits in the slit stretching method is 0.3-3 cm, and the width is 10-60 cm. It is understood that the width of the slit refers to the distance of the slit parallel to the long side direction of the nozzle. The length of the slit can be 10-60 cm. The length of the slit refers to the distance along the fiber ejection direction.

According to the present invention, preferably, a high-speed air flow is used to stretch the spun fiber in the slit, wherein the flow rate of the high-speed air flow is 2500-5500 m/min, preferably 3000-5000 m/min.

The third aspect of the present invention provides a polyester fiber cloth, which contains the above-mentioned composite polyester fiber or the composite polyester fiber made by the above-mentioned method. In some embodiments, the polyester fiber cloth is made of the above-mentioned composite polyester fiber, for example, the polyester fiber cloth is prepared from the above-mentioned composite polyester fiber. In some embodiments, the polyester fiber cloth may only contain the above-mentioned composite polyester fiber, but not other fibers. According to the present invention, preferably, the polyester fiber cloth is a non-woven fabric.

According to the present invention, preferably, the polyester fiber cloth has a thickness of 0.35-0.6 mm, preferably 0.4-0.5 mm, and an area density of 70-160 g/m ² , preferably 80-130 g/m ² .

In the present invention, the thickness of the polyester fiber cloth can be 0.35 mm, 0.4 mm, 0.41 mm, 0.42 mm, 0.43 mm, 0.44 mm, 0.45 mm, 0.46 mm, 0.47 mm, 0.48 mm, 0.49 mm, 0.50 mm, 0.51 mm, 0.52 mm, 0.53 mm, 0.54 mm, 0.55 mm, 0.56 mm, 0.57 mm, 0.58 mm, 0.59 mm or 0.60 mm, and a range formed by any two of the above points. The surface density of the polyester fiber can be 70 g/ ^m2 , 80 g/ ^m2 , 85 g/ ^m2 , 90 g/ ^m2 , 95 g/ ^m2 , 100 g/ ^m2 , 105 g/m2, 110 g/ ^m2 , ¹¹⁵ g/m2, 120 g/ ^m2 , 125 g/ ^m2 , 130 g/ ^m2 , 135 g/ ^m2 , 140 g/ ^m2 , 150 g/ ^m2 or 160 g/ ^m2 , and a range consisting of ^any two of the above points.

Generally, the polyester fiber cloth of the present invention can be prepared from the composite polyester fiber of the present invention by various methods generally known in the art.

In the present invention, the standard deviation of the fiber diameter in the polyester fiber cloth is ≤2.4 μm, preferably ≤2.2 μm, and more preferably ≤2.0 μm. The test method for the standard deviation of the fiber diameter in the polyester fiber cloth is: take a scanning electron microscope (SEM) photo of the polyester fiber cloth, and measure the diameters of 50 randomly selected fibers from the photo (each fiber is measured once) with an accuracy of 0.1 μm, and then calculate the standard deviation of these diameter values according to the standard deviation calculation method. The calculation formula of the diameter standard deviation is as follows: ,

Where n is the number of composite polyester fiber samples (i.e. 50), i is the fiber sample number, d _i is the diameter of the fiber numbered i, It is the arithmetic mean of the diameters of 50 fiber samples.

The standard deviation of the fiber diameter in the polyester fiber cloth may be 2.4 μm, 2.3 μm, 2.2 μm, 2.1 μm, 2.0 μm, 1.9 μm, 1.8 μm, 1.6 μm, 1.4 μm, 1.2 μm, 1 μm, 0.8 μm, 0.6 μm, 0.4 μm, 0.2 μm, 0.1 μm, and a range consisting of any two of the above points, such as 0.1-2.4 μm.

The method for testing the surface density of the fiber cloth in the present invention is: using a ten-thousandth balance to weigh the fiber cloth with an area of 1 ^cm2 , and then dividing the mass ratio of the fiber cloth by its area. In the present invention, the thickness of the fiber cloth is measured by a thickness gauge, for example, a dial-type thickness gauge can be used for measurement. For example, a Mitutoyo 547-313 thickness gauge of Japan can be used.

The fourth aspect of the present invention provides a method for preparing the above-mentioned polyester fiber cloth, which comprises laying out the composite polyester fiber of the present invention and hot pressing to obtain the polyester fiber cloth.

According to the present invention, preferably, the temperature of the hot pressing is 180-220°C.

Instead of using long fibers, the method may also include wet-laying and heat-treating a slurry of short fibers containing polyester fibers in sequence.

In the method, the length of the short polyester fiber can be 1 mm, 2 mm, 3 mm, 4 mm, 5 mm, 6 mm, 7 mm or 8 mm, and a range consisting of any two of the above points.

Preferably, the length of the short polyester fibers is 1-4 mm, more preferably 2-3 mm.

Preferably, the content of polyester fiber in the slurry containing polyester fiber is 5-30 wt %, for example, 5 wt %, 8 wt %, 10 wt %, 12 wt %, 14 wt %, 16 wt %, 18 wt %, 20 wt %, 25 wt %, 30 wt % and a range consisting of any two of the above, more preferably 10-20 wt %.

According to the present invention, preferably, the slurry containing polyester fiber further includes a dispersant, and the dispersant is at least one of polyethylene glycol, fatty alcohol and polyacrylamide.

According to the present invention, preferably, the content of the dispersant in the slurry containing the polyester fiber is 0.05-2% by weight; for example, 0.05% by weight, 0.5% by weight, 0.8% by weight, 0.9% by weight, 1% by weight, 1.1% by weight, 1.2% by weight, 1.3% by weight, 1.4% by weight, 1.5% by weight, 1.8% by weight, 2% by weight, and a range consisting of any two of the above.

According to the present invention, preferably, the dispersant is a combination of polyethylene glycol, fatty alcohol and polyacrylamide. More preferably, the weight ratio of the polyethylene glycol, fatty alcohol and polyacrylamide is 1:0.5-2:0.5-2, more preferably 1:1-1.2:1-1.2.

In the present invention, the weight ratio of polyethylene glycol to fatty alcohol can be 1:0.5, 1:0.7, 1:0.9, 1:1, 1:1.1, 1:1.2, 1:1.3, 1:1.5, 1:2, and a range consisting of any two of the above points.

In the present invention, the weight ratio of polyethylene glycol to polyacrylamide can be 1:0.5, 1:0.7, 1:0.9, 1:1, 1:1.1, 1:1.2, 1:1.3, 1:1.5, 1:2, and a range consisting of any two of the above points.

According to the present invention, preferably, the molecular weight of the polyethylene glycol is 400-4000 g/mol (for example, 400 g/mol, 500 g/mol, 600 g/mol, 700 g/mol, 800 g/mol, 900 g/mol, 1000 g/mol, 1500 g/mol, 1600 g/mol, 1700 g/mol, 2000 g/mol, and a range consisting of any two of the above), more preferably 500-2000 g/mol.

According to the present invention, preferably, the fatty alcohol is a fatty alcohol having 3-12 carbon atoms, more preferably, the number of hydroxyl groups in the fatty alcohol is 1-3; further preferably, the fatty alcohol is at least one of octanol, heptanol, hexanol, pentanol and butanol.

According to the present invention, preferably, the molecular weight of the polyacrylamide is 50000-5000000 g/mol (for example, 50000 g/mol, 100000 g/mol, 150000 g/mol, 180000 g/mol, 200000 g/mol, 220000 g/mol, 240000 g/mol, 250000 g/mol, 300000 g/mol, 400000 g/mol, 1000000 g/mol, 5000000 g/mol, and a range consisting of any two of the above), more preferably 200000-250000 g/mol.

In the present invention, unless otherwise specified, molecular weight refers to number average molecular weight.

According to the present invention, preferably, the temperature of the heat treatment is 180-220°C.

The fifth aspect of the present invention provides a fiber cloth carrier for cell culture, wherein the fiber cloth carrier is made of the above-mentioned polyester fiber cloth. In some embodiments, preferably, the fiber cloth carrier is a sheet-like fiber cloth carrier.

In the present invention, the polyester fiber cloth of the present invention can be pretreated and/or sterilized to prepare the fiber cloth carrier.

Preferably, the pretreatment comprises soaking the polyester fiber cloth in a hydrogen peroxide solution.

Preferably, the sterilization includes at least one of ethylene oxide sterilization, high temperature steam sterilization and irradiation sterilization, preferably high temperature steam sterilization.

According to the present invention, preferably, the conditions for high temperature steam sterilization include: a temperature of 115-135°C, such as 115°C, 120°C, 125°C, 130°C, 135°C, and a range consisting of any two of the above points, and a time of 10-60 min, such as 10 min, 20 min, 30 min, 40 min, 50 min, 60 min, and a range consisting of any two of the above points.

According to the present invention, preferably, the conditions for irradiation sterilization include: irradiating the pretreated fiber cloth with high-energy electron rays, the irradiation dose is 5-30 kGy, and the irradiation time is within 10 s.

According to the present invention, preferably, the fiber cloth is pretreated before sterilization. In some embodiments, the pretreatment method can be to pretreat the fiber cloth with a hydrogen peroxide solution.

According to the present invention, preferably, the concentration _{of H2O2} in the hydrogen peroxide solution is 5-10 wt%.

According to the present invention, preferably, the pretreatment conditions include: a temperature of 15-40°C and a time of 0.5-4h.

According to the present invention, preferably, the pre-treated fiber cloth can be cleaned under ultrasound and then sterilized.

According to the present invention, preferably, the cleaning agent used for cleaning is water, preferably double distilled water and/or ultrapure water.

According to the present invention, preferably, the cells are adherent cells, preferably Vero cells and/or L929 cells.

In some embodiments, the fiber cloth carrier optionally comprises or is made of multiple layers, preferably 2-7 layers, of the polyester fiber cloth of the present invention. Preferably, there are welding lines, such as 2-5 welding lines, between the multiple layers of polyester fiber cloth. Preferably, the shortest distance between any two welding lines is > 0.5 mm. More preferably, the welding lines are straight lines. In the present invention, the welding lines refer to the lines formed by the welding path, and welding refers to the use of heat to partially weld multiple layers of polyester fiber cloth.

In some embodiments, the fiber cloth carrier is preferably circular, elliptical or rectangular. Preferably, the diameter of the circle, the major axis of the ellipse or the length of the rectangle is greater than or equal to 2 mm and less than or equal to 15 mm.

A sixth aspect of the present invention provides a method for preparing the above-mentioned fiber cloth carrier, wherein the method comprises: pre-treating and/or sterilizing the polyester fiber cloth.

Preferably, the pretreatment comprises soaking the polyester fiber cloth in a hydrogen peroxide solution.

Preferably, the sterilization includes at least one of ethylene oxide sterilization, high temperature steam sterilization and irradiation sterilization, preferably high temperature steam sterilization.

The seventh aspect of the present invention provides the use of the composite polyester fiber of the present invention or the composite polyester fiber prepared by the method for preparing the composite polyester fiber of the present invention in preparing a fiber cloth carrier for cell culture.

An eighth aspect of the present invention provides an application of the polyester fiber cloth or fiber cloth carrier of the present invention in cell culture.

According to the present invention, preferably, the application can increase the cellular sugar metabolism rate. Preferably, using the fiber cloth of the present invention for cell culture can make the cellular sugar metabolism rate above 15 g/day, such as 15-35 g/day, preferably 20-33 g/day, and more preferably 27-32 g/day.

Preferably, the cells are adherent cells, more preferably Vero cells and/or L929 cells.

The ninth aspect of the present invention provides a method for culturing cells, which comprises: sterilizing the polyester fiber cloth of the present invention and placing it in a culture solution as a carrier, or placing the fiber cloth carrier of the present invention in a culture solution as a carrier, and inoculating cells on the carrier and culturing in vitro; preferably, the cells are adherent cells, more preferably Vero cells and/or L929 cells.

The present invention will be described in detail below through examples. In the following examples,

Unless otherwise specified, the blown sheet comes from Changzhou Jier Precision Machinery Manufacturing Co., Ltd.

The Sb content in the core material and the skin material is less than 20 ppm.

The room temperature is about "15-20℃".

In the present invention, the melting point is determined by placing about 5 mg of a sample in a differential scanning calorimeter (DSC) instrument (TA's Q100), heating the sample from 20°C to 300°C at a heating rate of 10°C/min, and observing the endothermic peak between 100°C and 260°C. The sample exhibits a single endothermic peak in this temperature range. The peak temperature is taken as the melting point.

In the following embodiments, the self-made polyester involved is prepared by mixing PTA (terephthalic acid, analytical grade), EG (ethylene glycol, analytical grade) and an optional comonomer Y (such as 1,4-cyclohexanedimethanol or isophthalic acid, analytical grade) in a reactor according to the required amount of the product, heating to 258-263°C, reacting for about 1 hour, then adding tetrabutyl titanium, raising the temperature to 275-290°C, and evacuating with an oil pump, and reacting for about 2 hours to obtain the desired polyester.

Specifically, the preparation method of the self-made polyester A is to add 166 g PTA and 64 g EG into a reactor, stir and heat to 258-263°C, react for about 1 hour, then add tetrabutyl titanate, raise the temperature to 275-290°C, and evacuate with an oil pump for about 2 hours to obtain the desired polyester. The preparation method of the self-made polyester B is to add 133 g PTA and 51 g EG into a reactor, stir and heat to 258-263°C, react for about 1 hour, then add tetrabutyl titanate, raise the temperature to 275-290°C, and evacuate with an oil pump for about 2 hours to obtain the desired polyester. The preparation method of the self-made polyester C is as follows: 133 g PTA, 35.4 g EG, and 34.6 g 1,4-cyclohexanedimethanol are added into a reaction kettle, stirred and heated to 258-263°C, reacted for about 1 hour, and then tetrabutyl titanium is added, the temperature is raised to 275-290°C, and vacuum is drawn with an oil pump, and the reaction is carried out for about 2 hours to obtain the desired polyester.

Embodiment 1

(1) Preparation of polyester fiber:

Homopolyethylene terephthalate (manufacturer: Sinopec Yizheng Chemical Fiber Co., Ltd., hereinafter referred to as "Yizheng Chemical Fiber", model FG600, intrinsic viscosity 0.675 dL/g, standard deviation of intrinsic viscosity 0.003 dL/g, melting point 261°C) was used as the core material. Copolyethylene terephthalate (Yizheng Chemical Fiber, model FG702, intrinsic viscosity 0.78 dL/g, standard deviation of intrinsic viscosity 0.006 dL/g, comonomer is cyclohexanedimethanol, comonomer content is 15 mol%, melting point 140°C) was used as the skin material. The mass ratio of the core material to the skin material is 3, and it is extruded from the nozzle plate at an outlet temperature of about 280°C. There are 48 nozzle holes on the nozzle plate, and each nozzle hole is in the shape of two concentric circles, with an inner diameter of 20 microns and a range (the diameter of the largest hole minus the diameter of the smallest hole) of 1.2 microns, and an inner diameter of 30 microns and a range of 1.1 microns. After the polymer melt is extruded from the nozzle, it is cooled by blowing with room temperature air at 1.5-1.8 m/s. The cooled fibers are evenly or nearly evenly fed into 5 steel round tubes (the inner diameter of the round tube is 2 cm, the distance between two adjacent round tubes is 3.5 cm, and the length of the round tube is 1 m). The cooled fibers are stretched in the round tube at a wind speed of about 4000-4500 m/min to obtain composite polyester fibers.

The average diameter of the composite polyester fiber was tested and the diameter standard deviation was calculated. The test results are shown in Table 1.

The test method for the average diameter of composite polyester fibers is as follows: randomly select 50 composite polyester fibers, randomly cut a small section, take a scanning electron microscope (SEM) (Hitachi S-4800) photo, and measure the diameter of each fiber from the photo, accurate to 0.1 μm, and then calculate the average value of these diameter values according to the average value calculation method; and calculate the standard deviation of these diameter values according to the standard deviation calculation method. The test results are shown in Table 1.

(2) Preparation of polyester fiber cloth:

The obtained polyester fiber is laid out on a mesh machine, and then the fiber cloth mesh of a certain thickness is hot pressed at 190-200°C using a non-embossed polished hot roller to obtain a fiber cloth. The test method for the surface density of the fiber cloth is: use a 1/10,000 balance to weigh the fiber cloth with an area of 1 ^cm2 , and then divide the mass ratio of the fiber cloth by its area; the test method for the thickness of the fiber cloth is: use a Mitutoyo thickness gauge (model 547-313) of Japan to measure. The surface density and thickness of the fiber cloth are shown in Table 1.

Embodiment 2

The method of Example 1 was followed, except that polyethylene terephthalate (Instrumental Chemical Fiber, Model BG804, intrinsic viscosity 0.801 dL/g, standard deviation of intrinsic viscosity 0.005 dL/g, comonomer cyclohexanedimethanol, comonomer content 0.75 mol%, melting point 237°C) was used as the skin material.

Embodiment 3

The method of Example 1 is followed, except that the mass ratio of the core layer material to the skin layer material is 2, the inner diameter of the inner circle of each nozzle hole of the nozzle plate is 18 microns, the range is 1.1 microns, and the inner diameter of the outer circle is 32 microns, the range is 1.6 microns.

Embodiment 4

The method of Example 1 is followed, except that the mass ratio of the core layer material to the skin layer material is 3.6.

Embodiment 5

The method of Example 1 is followed, except that the mass ratio of the core layer material to the skin layer material is 1.6.

Embodiment 6

The method of Example 1 is followed, except that the cooled fiber is fed into a slit with a spacing of 1 cm, the width of the slit is 50 cm, and the length is 50 cm. The cooled fiber is stretched at a wind speed of 3500-4000 m/min to obtain polyester fiber.

Embodiment 7

The method of Example 6 is followed, except that copolyethylene terephthalate (Instrumental Chemical Fiber, Model BG804, intrinsic viscosity 0.801 dL/g, standard deviation of intrinsic viscosity 0.005 dL/g, comonomer cyclohexanedimethanol, based on all monomers, comonomer content 0.75 mol%, melting point 237°C) is used as the skin material.

Embodiment 8

The method of Example 6 is followed, except that the mass ratio of the core layer material to the skin layer material is 2, the inner diameter of the inner circle of the nozzle hole of the nozzle plate is 18 microns, the range is 1.1 microns, and the inner diameter of the outer circle of the nozzle hole is 32 microns, the range is 1.6 microns.

Embodiment 9

The method of Example 6 is followed, except that the mass ratio of the core layer material to the skin layer material is 3.6.

Embodiment 10

The method of Example 6 is followed, except that the mass ratio of the core layer material to the skin layer material is 1.6.

Embodiment 11

(1) Preparation of polyester fiber:

According to step (1) in Example 1, polyester fiber is obtained.

(2) Preparation of polyester fiber cloth:

The polyester fiber obtained in step (1) is cut into pieces with a cutting length of 3±0.1 mm to obtain polyester staple fibers. The polyester staple fibers are uniformly mixed with water and a dispersant to obtain a slurry containing polyester staple fibers, wherein the content of polyester staple fibers in the slurry containing polyester staple fibers is 15% by weight, and the content of the dispersant is 1% by weight. The dispersant is polyethylene glycol, fatty alcohol and polyacrylamide in a weight ratio of 1:1:1, the molecular weight of the polyethylene glycol is 800 g/mol, the fatty alcohol is n-octanol, and the molecular weight of the polyacrylamide is 200000 g/mol. The slurry containing polyester staple fibers is then transported to the web forming area of a wet web forming machine, water is filtered through a screen, and the polyester staple fibers form a fiber web on the screen. After the fiber web is dried, it is hot-pressed at 190-200°C using a non-embossed polished hot roll to obtain a fiber cloth.

Embodiment 12

The method of Example 11 is followed, except that in step (1), copolyethylene terephthalate (instrumental chemical fiber, model BG804, intrinsic viscosity 0.801 dL/g, intrinsic viscosity standard deviation 0.005 dL/g, comonomer is cyclohexanedimethanol, based on all monomers, the comonomer content is 0.75 mol%, melting point 237°C) is used as the skin material.

Embodiment 13

The method of Example 11 is followed, except that in step (1), the mass ratio of the core layer material to the skin layer material is 2, the inner diameter of the inner circle of the nozzle hole of the nozzle plate is 18 μm, the range is 1.1 μm, and the inner diameter of the outer circle of the nozzle hole is 32 μm, the range is 1.6 μm.

Embodiment 14

The method of Example 11 is followed, except that in step (1), the mass ratio of the core layer material to the skin layer material is 3.6.

Embodiment 15

The method of Example 11 is followed, except that in step (1), the mass ratio of the core layer material to the skin layer material is 1.6.

Embodiment 16

The method of Example 11 is followed, except that in step (2), the polyester fiber obtained in step (1) is cut into pieces with a cutting length of 2±0.1 mm to obtain polyester staple fibers. The polyester staple fibers are uniformly mixed with water and a dispersant to obtain a slurry containing polyester staple fibers, wherein the content of polyester staple fibers in the slurry containing polyester staple fibers is 18% by weight, and the content of the dispersant is 1.5% by weight. The dispersant is polyethylene glycol, fatty alcohol and polyacrylamide in a weight ratio of 1:1.2:1.2, the molecular weight of the polyethylene glycol is 1600 g/mol, the fatty alcohol is n-octanol, and the molecular weight of the polyacrylamide is 250000 g/mol.

Embodiment 17

The method of Example 11 is followed, except that in step (2), the fatty alcohol and polyacrylamide are replaced by equal weights of polyethylene glycol.

Comparative Example 1

The method of Example 1 is followed, except that the mass ratio of the core layer material to the skin layer material is 1.

Comparative Example 2

The method of Example 1 is followed, except that the mass ratio of the core layer material to the skin layer material is 5.

Comparative Example 3

The method of Example 1 was followed, except that the raw material used for homopolyethylene terephthalate was self-made polyester A, with an intrinsic viscosity of 0.51 dL/g, a standard deviation of the intrinsic viscosity of 0.01 dL/g, and a melting point of 260°C.

Comparative Example 4

The method of Example 1 is followed, except that the nozzle holes of the nozzle plate are not concentric circles, but are parallel structures that divide a circle into two. The inner diameter of the circular nozzle hole is 30 microns, and the range is 1.1 microns.

Comparative Example 5

The method of Example 1 is followed, except that a larger range of the hole diameter of the nozzle plate is used, the inner diameter of the inner circle is 20 microns, the range is 3.6 microns, and the inner diameter of the outer circle is 30 microns, the range is 5.3 microns.

Comparative Example 6

The method of Example 1 was followed, except that homopolyethylene terephthalate was replaced by fiberized BG85, which had an intrinsic viscosity of 0.879 dL/g, a standard deviation of the intrinsic viscosity of 0.005 dL/g, and a melting point of 248°C.

Comparative Example 7

The method of Example 1 is followed, except that homopolyethylene terephthalate is replaced by self-made polyester B, which has an intrinsic viscosity of 0.675 dL/g, a standard deviation of the intrinsic viscosity of 0.031 dL/g, and a melting point of 260°C; copolyethylene terephthalate is replaced by self-made polyester C, which has an intrinsic viscosity of 0.782 dL/g, a standard deviation of the intrinsic viscosity of 0.025 dL/g, and a melting point of 143°C; the comonomer is cyclohexanedimethanol, and the comonomer content is 15 mol%.

Comparative Example 8

The method of Example 1 was followed, except that homopolyethylene terephthalate was replaced by BG804 of instrumented fiber with an intrinsic viscosity of 0.801 dL/g, a standard deviation of the intrinsic viscosity of 0.005 dL/g, and a melting point of 237°C.

Comparative Example 9

The method of Example 1 was followed, except that the copolymerized terephthalate was replaced by the fiberized BG85, which had an intrinsic viscosity of 0.879 dL/g, a standard deviation of the intrinsic viscosity of 0.005 dL/g, and a melting point of 248°C.

Comparative Example 10

The method of Example 1 is followed, except that the wind speed in the circular tube is 1500-2000 m/min.

Comparative Example 11

The method of Example 1 is followed, except that the molten filament does not enter the round tube after cooling, but is stretched and laid on the mesh using the swing wire method.

Compare Example 12-13

The method of Example 1 is followed, except that different surface densities and thicknesses of the fiber cloth are obtained by adjusting the process parameters of mesh laying and hot pressing. The surface density and thickness of the fiber cloth are shown in Table 1.

Comparative Example 14

The method of Example 6 is followed, except that the mass ratio of the core layer material to the skin layer material is 1.

Comparative Example 15

The method of Example 6 is followed, except that the mass ratio of the core layer material to the skin layer material is 5.

Comparative Example 16

The method of Example 6 was followed, except that the raw material used for homopolyethylene terephthalate was self-made polyester A, with an intrinsic viscosity of 0.51 dL/g, a standard deviation of the intrinsic viscosity of 0.01 dL/g, and a melting point of 260°C.

Comparative Example 17

The method of Example 6 is followed, except that the nozzle holes of the nozzle plate are not concentric circles, but are parallel structures that divide a circle into two. The inner diameter of the circular nozzle hole is 30 microns, and the range is 1.1 microns.

Comparative Example 18

The method of Example 6 is followed, except that a larger range of the hole diameter of the nozzle plate is used, the inner diameter of the inner circle is 20 microns, the range is 3.6 microns, and the inner diameter of the outer circle is 30 microns, the range is 5.3 microns.

Comparative Example 19

The method of Example 6 is followed, except that the raw material model of the homopolyethylene terephthalate used is BG85 of the instrumental fiber, the intrinsic viscosity is 0.879 dL/g, the standard deviation of the intrinsic viscosity is 0.005 dL/g, and the melting point is 248°C.

Comparative Example 20

The method of Example 6 is followed, except that the homopolyethylene terephthalate is replaced by self-made polyester B, which has an intrinsic viscosity of 0.675 dL/g, a standard deviation of the intrinsic viscosity of 0.031 dL/g, and a melting point of 260°C; the copolymerized polyethylene terephthalate is replaced by self-made polyester C, which has an intrinsic viscosity of 0.782 dL/g, a standard deviation of the intrinsic viscosity of 0.025 dL/g, and a melting point of 143°C; the comonomer is cyclohexanedimethanol, and the comonomer content is 15 mol%.

Comparative Example 21

The method of Example 6 was followed, except that homopolyethylene terephthalate was replaced by BG804 of characterization fiber, with an intrinsic viscosity of 0.801 dL/g, a standard deviation of the intrinsic viscosity of 0.005 dL/g, and a melting point of 237°C.

Comparative Example 22

The method of Example 6 was followed, except that the copolymerized terephthalate was replaced by the fiberized BG85, which had an intrinsic viscosity of 0.879 dL/g, a standard deviation of the intrinsic viscosity of 0.005 dL/g, and a melting point of 248°C.

Comparative Example 23

The method of Example 6 is followed, except that the wind speed in the slit is 1500-2000 m/min.

Comparative Example 24

The method of Example 11 is followed, except that the mass ratio of the core layer material to the skin layer material is 1.

Comparative Example 25

The method of Example 11 was followed, except that homopolyethylene terephthalate was replaced by self-made polyester A, which had an intrinsic viscosity of 0.51 dL/g, a standard deviation of the intrinsic viscosity of 0.01 dL/g, and a melting point of 260°C.

Comparative Example 26

The method of Example 11 is followed, except that a larger range of the hole diameter of the nozzle plate is used, the inner diameter of the inner circle is 20 microns, the range is 3.6 microns, and the inner diameter of the outer circle is 30 microns, the range is 5.3 microns.

Comparative Example 27

The method of Example 11 is followed, except that in step (2), the polyester fiber obtained in step (1) is cut into short fibers having a cutting length of 10±0.1 mm to obtain polyester staple fibers.

Comparative Example 28

The method of Example 1 is followed, except that the speed of the screen-laying machine receiving the curtain is accelerated to three times the original speed during screen laying.

Test example

Pretreatment and sterilization of fiber cloth and sheet-like fiber cloth carrier: soak in 7 wt% hydrogen peroxide solution at room temperature for 60 min; then use ultrapure water for ultrasonic cleaning and dry at 80°C. After drying, sterilize the fiber cloth and sheet-like fiber cloth carrier with high-temperature steam at a temperature of 121°C for 60 min.

Then the sugar metabolism ability of Vero cells was tested when the sterilized fiber cloth or fiber cloth sheet carrier was used as the carrier. The test method of sugar metabolism is as follows: the fiber cloth or fiber cloth sheet carrier was cut into small pieces of 6 mm×6 mm, 100 g of 6 mm×6 mm fiber cloth or fiber cloth sheet carrier, 8L PBS buffer and about 200mL 199 culture medium (purchased from Beijing Tsinghua Tianyi Biotechnology Co., Ltd.) were placed in a 10L basket reactor, and 10% of high-quality newborn calf serum (purchased from Lanzhou Minhai Bioengineering Co., Ltd.) relative to the culture medium was added, and an appropriate amount of 20% by weight glucose was added to make the total glucose concentration 0.5% by weight. Vero cells (purchased from the National Biomedical Laboratory Cell Resource Bank, 4th generation Vero cells, 0.5×10 ⁶ cells per 1 cm ² of fiber cloth) were inoculated and cultured at 37°C with aeration. During the culture period, pH was set to 7.3, DO (dissolved oxygen as a percentage of saturated dissolved oxygen) was 60%, and carbon dioxide concentration was 5%. The cells were stirred slowly and cultured for 7 days. Samples were taken every 24 h and the glucose content was detected using a glucose test kit (purchased from Nanjing Jiancheng Bioengineering Institute), and the glucose concentration was supplemented to 0.5 wt % with a 20 wt % glucose solution. The measured decrease in glucose content was converted to a glucose consumption rate value in g/day. The glucose consumption rate was plotted against the culture time, and the value at 100 h was taken as the 100 h sugar metabolism rate value. The 100 h sugar metabolism rate values are shown in Table 1.

Table 1 Average diameter of composite polyester fiber (μm) Diameter standard deviation of composite polyester fiber (μm) Fiber fabric density (g/m ² ) Fiber cloth thickness (mm) Standard deviation of fiber diameter in fiber cloth (μm) 100h sugar metabolism rate (g/day) Whether it satisfies formula (1) Embodiment 1 twenty two 1.4 118 0.42 1.5 30 yes Embodiment 2 18 1.5 118 0.42 1.7 28 yes Embodiment 3 29 1.6 120 0.44 1.7 28 yes Embodiment 4 18 1.4 118 0.41 1.6 19 no Embodiment 5 29 1.8 119 0.43 1.9 twenty one no Embodiment 6 twenty one 1.9 117 0.42 2.0 26 yes Embodiment 7 18 1.8 118 0.43 1.9 twenty three yes Embodiment 8 28 2.2 116 0.43 2.3 25 yes Embodiment 9 18 2.1 119 0.44 2.2 16 no Embodiment 10 29 2.1 118 0.43 2.2 17 no Embodiment 11 twenty two 1.4 115 0.43 1.6 29 yes Embodiment 12 18 1.5 114 0.43 1.6 27 yes Embodiment 13 29 1.6 116 0.44 1.6 27 yes Embodiment 14 18 1.4 115 0.44 1.5 twenty two no Embodiment 15 29 1.8 113 0.42 2.0 twenty one no Embodiment 16 twenty two 1.4 115 0.42 1.6 27 yes Embodiment 17 twenty two 1.4 113 0.41 1.7 16 yes Comparative Example 1 14 3.5 116 0.42 4.1 5 yes Comparative Example 2 28 2.6 119 0.43 2.9 3 yes Comparative Example 3 27 4.1 119 0.44 4.4 8 yes Comparative Example 4 twenty two 4.6 116 0.41 4.9 1 yes Comparative Example 5 twenty two 6.1 117 0.41 6.8 5 yes Comparative Example 6 26 3.2 119 0.44 3.4 2 yes Comparative Example 7 20 5.1 118 0.42 5.4 3 yes Comparative Example 8 20 3.1 119 0.44 3.2 8 yes Comparative Example 9 25 2.4 118 0.42 2.7 6 yes Comparative Example 10 28 2.4 117 0.42 2.8 9 yes Comparative Example 11 38 6.8 118 0.42 7.3 1 yes Comparative Example 12 twenty two 1.4 236 0.84 1.6 9 yes Comparative Example 13 twenty two 1.4 223 0.31 1.6 2 yes Comparative Example 14 15 3.9 117 0.41 4.3 3 yes Comparative Example 15 29 4.2 120 0.45 4.5 1 yes Comparative Example 16 28 5.2 118 0.43 5.7 1 yes Comparative Example 17 twenty three 4.8 117 0.42 5.2 3 yes Comparative Example 18 twenty two 6.5 116 0.40 6.9 2 yes Comparative Example 19 25 4.1 118 0.42 4.4 6 yes Comparative Example 20 twenty one 4.9 117 0.42 5.1 2 yes Comparative Example 21 20 3.5 119 0.44 3.8 4 yes Comparative Example 22 27 2.9 117 0.41 3.1 3 yes Comparative Example 23 28 2.8 116 0.41 3.2 5 yes Comparative Example 24 14 3.5 115 0.42 3.7 3 yes Comparative Example 25 27 4.1 116 0.43 4.2 2 yes Comparative Example 26 twenty two 6.1 115 0.43 6.6 3 yes Comparative Example 27 twenty two 1.4 116 0.43 1.6 5 yes Comparative Example 28 twenty two 1.4 40 0.15 1.6 8 yes

It can be seen from the results in Table 1 that the diameter standard deviation of the composite polyester fiber of the present invention or the composite polyester fiber prepared by the method of the present invention is small. The carrier prepared by the composite polyester fiber of the present invention can significantly improve the sugar metabolism ability of cells.

The preferred embodiments of the present invention are described in detail above, but the present invention is not limited thereto. Within the technical conception of the present invention, the technical solution of the present invention can be subjected to a variety of simple modifications, including combining various technical features in any other suitable manner, and these simple modifications and combinations should also be regarded as the contents disclosed by the present invention and belong to the protection scope of the present invention.

without

without

Claims (26)

A composite polyester fiber, characterized in that the composite polyester fiber has a core-skin structure; wherein the mass ratio of the core material to the skin material is x, and 1.5≤x≤4, preferably 2≤x≤3.6, more preferably 2≤x≤3; and wherein the average diameter D of the composite polyester fiber is 10-40 μm, preferably 17-32 μm, more preferably 18-30 μm, and the diameter standard deviation is ≤2.2 μm, preferably ≤2.0 μm, more preferably ≤1.8 μm. The composite polyester fiber as described in claim 1, wherein the melting point of the core material is higher than that of the skin material by more than 20°C, preferably more than 22°C; the intrinsic viscosity of the skin material is higher than that of the core material by 0.01-0.18 dL/g, preferably 0.01-0.15 dL/g, and the standard deviation of the intrinsic viscosity of the core material and the skin material is ≤0.02 dL/g, preferably ≤0.015 dL/g; and/or The core layer material is homopolyethylene terephthalate, copolyethylene terephthalate or a combination thereof, preferably homopolyethylene terephthalate, and the skin layer material is homopolyethylene terephthalate, copolyethylene terephthalate or a combination thereof, preferably copolyethylene terephthalate. The composite polyester fiber as described in any one of claims 1 to 2, wherein the mass ratio x of the core layer material to the skin layer material of the composite polyester fiber and the average diameter D of the composite polyester fiber satisfy the following formula (1): Formula (1), wherein the value of the average diameter D of the composite polyester fiber used in formula (1) is in micrometers. The composite polyester fiber as described in any one of claims 1 to 3, wherein the core layer material is homopolyethylene terephthalate; preferably, the intrinsic viscosity of the homopolyethylene terephthalate is 0.6-0.8 dL/g, preferably 0.62-0.8 dL/g, more preferably 0.65-0.7 dL/g, and the melting point is above 240°C, more preferably 245-280°C, and/or preferably, the standard deviation of the intrinsic viscosity of the homopolyethylene terephthalate is ≤0.015 dL/g, preferably ≤0.01 dL/g, more preferably ≤0.005 dL/g. The composite polyester fiber as described in any one of claims 1 to 4, wherein the skin material is copolyethylene terephthalate; preferably, the copolyethylene terephthalate has an intrinsic viscosity of 0.601-0.9 dL/g, preferably 0.61-0.9 dL/g, more preferably 0.61-0.82 dL/g, and a melting point below 240°C, more preferably 130-240°C, and/or preferably, the standard deviation of the intrinsic viscosity of the copolyethylene terephthalate is ≤0.015 dL/g, preferably ≤0.01 dL/g. The composite polyester fiber as described in claim 5, wherein the copolyethylene terephthalate comprises structural units from terephthalic acid, ethylene glycol and comonomer Y, and the content of structural units from comonomer Y in the copolyethylene terephthalate is 0.6-20 mol%, preferably 0.6-18 mol%, more preferably 0.7-15 mol%; Preferably, the comonomer Y is selected from dicarboxylic acids, diols and tetraols, preferably at least one selected from isophthalic acid, phthalic acid, butanediol, hexanediol, cyclohexanedimethanol and pentanediol. A method for preparing a composite polyester fiber as described in any one of claims 1 to 6, characterized in that the method comprises: melting a core layer material and a skin layer material separately to obtain a melt, and spinning the melt to obtain the composite polyester fiber. A method for preparing composite polyester fiber as described in claim 7, wherein the shape of each nozzle hole of the nozzle plate used in the spinning process is two concentric circles, the difference between the maximum diameter and the minimum diameter of all inner circles of the nozzle holes is ≤2 μm, and the difference between the maximum diameter and the minimum diameter of all outer circles of the nozzle holes is ≤2 μm, and/or the inner diameter of the inner circle of the nozzle hole is 15-25 μm, and the inner diameter of the outer circle is 25-35 μm. A method as described in any one of claims 7 to 8, wherein the method comprises: (1) feeding the molten core layer material and the skin layer material into a spinneret for spinning to obtain a filamentary melt having a skin-core structure; (2) cooling the filamentary melt having a skin-core structure to obtain spun fibers; and (3) stretching the spun fibers to obtain the composite polyester fibers. The method as claimed in claim 9, wherein, the spun fibers are stretched using high-speed airflow, preferably a high-speed airflow of 2500-5500 m/min; preferably, the stretching includes at least one of a round tube stretching method and a slit stretching method. A method as claimed in claim 10, wherein, in the slit stretching method, the slits have a spacing of 0.3-3 cm, a width of 10-60 cm and a length of 10-60 cm; or In the round tube method, the inner diameter of the round tube is 0.5-5 cm, the distance between two adjacent round tubes is 0.5-10 cm, and the length of the round tube is 0.1-2 m. A polyester fiber cloth, characterized in that the polyester fiber cloth comprises the composite polyester fiber as described in any one of claims 1 to 6 or the composite polyester fiber made by the method as described in any one of claims 7 to 11. The polyester fiber cloth as described in claim 12, wherein the thickness of the polyester fiber cloth is 0.35-0.6 mm, preferably 0.4-0.5 mm, and the surface density is 70-160 g/m ² , preferably 80-130 g/m ² ; and/or the polyester fiber cloth is a non-woven fabric; and/or the standard deviation of fiber diameter in the polyester fiber cloth is ≤2.4 μm, preferably ≤2.2 μm, and more preferably ≤2.0 μm. A method for preparing the polyester fiber cloth as described in any one of claims 12-13, comprising laying out the composite polyester fiber and hot pressing to obtain the polyester fiber cloth. A method as described in claim 14, wherein the temperature of the hot pressing is 180-220°C. A method as described in claim 14 or 15, wherein a slurry of staple fibers containing the composite polyester fibers is wet-laid. The method of claim 16, wherein the content of the composite polyester fiber in the slurry is 5-30% by weight, preferably 10-20% by weight, and/or The slurry further includes a dispersant, preferably, the dispersant is at least one of polyethylene glycol, fatty alcohol and polyacrylamide, and/or preferably, the content of the dispersant in the slurry is 0.05-2% by weight. The method as claimed in claim 17, wherein the dispersant is a combination of polyethylene glycol, fatty alcohol and polyacrylamide, wherein the weight ratio of the polyethylene glycol, fatty alcohol and polyacrylamide is 1:0.5-2:0.5-2; More preferably, the molecular weight of the polyethylene glycol is 400-4000 g/mol; More preferably, the fatty alcohol is a fatty alcohol having 3-12 carbon atoms, and further preferably, the number of hydroxyl groups in the fatty alcohol is 1-3; and/or More preferably, the molecular weight of the polyacrylamide is 50000-5000000 g/mol. A fiber cloth carrier for cell culture, characterized in that the fiber cloth carrier is made of the polyester fiber cloth as described in any one of claims 12 to 13 or is prepared by the method for preparing polyester fiber cloth as described in any one of claims 14 to 18. A fiber cloth carrier as described in claim 19, wherein the fiber cloth carrier is made by pre-treating and/or sterilizing the polyester fiber cloth; Preferably, the pre-treatment includes soaking the polyester fiber cloth in a hydrogen peroxide solution; and/or Preferably, the sterilization includes at least one of ethylene oxide sterilization, high temperature steam sterilization and irradiation sterilization, preferably high temperature steam sterilization. The fiber cloth carrier as described in any one of claims 19 to 20 comprises multiple layers, preferably 2-7 layers, of the polyester fiber cloth as described in any one of claims 12 to 13; preferably, there are welding lines between the polyester fiber cloths, preferably, the shortest distance between any two welding lines is greater than 0.5 mm, and more preferably, the welding lines are straight lines. A fiber cloth carrier as described in any one of claims 19 to 21, wherein the fiber cloth carrier is circular, elliptical or rectangular; preferably, the diameter of the circle, the major axis of the ellipse or the length of the rectangle is greater than or equal to 2 mm and less than or equal to 15 mm. A method for preparing a fiber cloth carrier as described in any one of claims 19 to 22, characterized in that the method comprises: pre-treating and/or sterilizing the polyester fiber cloth; Preferably, the pre-treatment comprises soaking the polyester fiber cloth in a hydrogen peroxide solution; and/or Preferably, the sterilization comprises at least one of ethylene oxide sterilization, high temperature steam sterilization and irradiation sterilization, preferably high temperature steam sterilization. Use of the composite polyester fiber as described in any one of claims 1 to 6 or the composite polyester fiber produced by the method as described in any one of claims 7 to 11 in preparing a fiber cloth carrier for cell culture. A use of the polyester fiber cloth as described in any one of claims 12 to 13 or the fiber cloth carrier as described in any one of claims 19 to 22 in cell culture, wherein the cells are preferably adherent cells, more preferably Vero cells and/or L929 cells. A method for culturing cells, characterized in that the method comprises: sterilizing the polyester fiber cloth as described in any one of claims 12 to 13 and placing it in a culture solution as a cell carrier, or placing the fiber cloth carrier as described in any one of claims 19 to 22 in a culture solution as a cell carrier, and inoculating cells on the cell carrier and culturing in vitro; preferably, the cells are adherent cells, more preferably Vero cells and/or L929 cells.