TW201900038A - Lipid absorption promoter - Google Patents

Abstract

The present invention addresses the problem of providing a fat absorption promoter which can be safely and easily taken and has an excellent effect of promoting fat absorption. Provided is a fat absorption promoter which comprises, as an active ingredient, a fat or oil containing [beta]-palmitic acid at a high concentration. The fat absorption promoter according to the present invention, which highly contributes to the digestion and absorption of fat, is appropriate for, for example, nutritional support and nutritional status improvement for infants.

Description

Lipid absorption enhancer

The present invention relates to a fat absorption enhancer.

Fatty acids, a constituent of fat, are one of the main sources of energy for humans, especially for young children. The fatty acid is combined with the triglyceride to form a fat. After ingestion, it is hydrolyzed by the pancreatic enzyme in the human body, and the fatty acid which is originally bound to the triglyceride α position and the α' position is a free fatty acid and can be absorbed by the human body. At this time, depending on the type of fatty acid and the position of binding to the triglyceride, it is considered that there is a possibility that the absorption of the free fatty acid differs, and various types of reviews have been conducted so far.

For example, JP-A-2002-180082 (Patent Document 1) discloses that an ω-3 fatty acid such as docosahexaenoic acid or eicosapentaenoic acid is bonded at a 2-position, and a medium-chain fatty acid is at 1, 3 The oil is combined with the position, and it is described that the oil-based omega-3 fatty acid has a good absorption rate. Further, JP-A-2006-521368 (Patent Document 2) discloses a lipid-improving agent comprising a triglyceride in which a highly unsaturated fatty acid is bonded at two positions of a triglyceride. [Prior Technical Paper] [Patent Document]

[Patent Document 1] JP-A-2002-180082 [Patent Document 2] Japanese Patent Publication No. 2006-521368

As mentioned above, a wide variety of substances have been explored to promote the absorption of fats and fatty acids. However, substances known to date do not necessarily achieve sufficient effects. Therefore, the present invention has been made in an effort to provide a fat absorption enhancer which is safe and easy to ingest and which has an excellent function of promoting fat absorption.

In order to solve the above problems, the present invention has focused on palmitic acid in fatty acids, particularly focusing on palmitic acid (hereinafter also referred to as β-palmitic acid) which binds to the β position of triglyceride, and discusses its characteristics in detail. . As a result, it has been found that fats and oils containing β-palmitic acid at a high concentration have an excellent fat-absorbing effect, and the present invention has been completed.

That is, the present invention is as follows. [1] A fat absorption enhancer and a composition for promoting fat absorption, which comprises a fat or oil containing β-palmitic acid as an active ingredient. [2] The fat absorption improving agent according to [1], wherein the ratio of palmitic acid in the β-position to the palmitic acid in the fat or oil is less than 98% with respect to the total amount of palmitic acid. [3] Use of a fat containing β-palmitic acid for producing a composition for promoting fat absorption, or for producing a fat absorption enhancer, or for promoting fat absorption, or as a fat absorption enhancer . [4] As used in [3], wherein the ratio of palmitic acid in the fat to the palmitic acid in the oil is 54% or more and less than 98%.

Further, the present invention also includes the following invention. [5] A method for promoting fat absorption, which comprises the steps of allowing a mammal to take in fat containing β-palmitic acid or administering it. [6] The method for promoting fat absorption according to [5], wherein the ratio of the palmitic acid at the β position to the total amount of palmitic acid in the fat or oil is 54% or more and less than 98%. [7] A fat containing β-palmitic acid, which is used for producing a composition for promoting fat absorption, or for producing a fat absorption enhancer, or for promoting fat absorption. [8] The oil according to [7], wherein the ratio of palmitic acid in the fat to the total amount of palmitic acid is 54% or more and less than 98%. [9] A food for infants and young children, which comprises a fat absorption enhancer containing a fat of β-palmitic acid as an active ingredient. [10] The food for infants and young children according to [9], wherein the ratio of the palmitic acid in the β-position relative to the total amount of palmitic acid is less than 94%. [11] A milk powder preparation comprising a fat absorption enhancer containing a fat of β-palmitic acid as an active ingredient. [12] The milk powder according to [11], wherein the ratio of the palmitic acid at the β position to the total amount of palmitic acid is less than 94%.

According to the present invention, a fat absorption enhancer having excellent effects can be provided. The β-palmitic acid of the active ingredient of the present invention has long-term eating experience and has been sufficiently confirmed to be safe. Further, the composition containing the fat absorption enhancer of the present invention has excellent digestibility and absorbability to fat, and is suitable for, for example, nutritional supplementation and improvement of nutritional status of infants and young children.

The present invention is a fat absorption enhancer containing a fat of β-palmitic acid as an active ingredient. Here, "fat absorption" is used to mean "lipid absorption". The β-palmitic acid constituting the active ingredient of the present invention is a substance in which palmitic acid is bonded to the β position of the triglyceride, and all compounds having the same chemical formula are contained. In addition, the β-palmitic acid may be a pure compound or a mixture with other substances. For example, β-position palmitic acid can be measured by a known method, and a raw material for confirming its presence can be used as it is. As a raw material (mixture) rich in β-palmitic acid, a representative example is lard, and this raw material can also be used. Further, for example, a commercially available product of β-palmitic acid may be purchased, and an appropriate other raw material may be blended.

The ratio of the palmitic acid at the β-position on the triglyceride (the ratio of the palmitic acid to the β position, the mass ratio) of the β-position-palmitic acid-containing fat or oil of the active ingredient of the present invention relative to the total amount of palmitic acid and triglyceride in the fat or oil. It is preferably 65% or more and 75% or less, more preferably 67% or more and 73% or less, and 68% or more and 72% or less. When the ratio is less than 65%, the effect as the fat absorption enhancer of the present invention cannot be sufficiently obtained. Moreover, when this ratio exceeds 75%, it cannot manufacture fully stably.

The fat absorption enhancer of the present invention can exert its function by oral ingestion or oral administration to a human-derived mammal. In the present invention, it is preferred to take it by diet.

The present invention is a use of a fat or oil containing β-palmitic acid for producing a composition for promoting fat absorption, for producing a fat absorption enhancer, or for promoting fat absorption. Further, the present invention is characterized by a method of promoting fat absorption using a fat containing β-palmitic acid. Further, the present invention relates to a fat or oil of β-palmitic acid which is used for producing a composition for promoting fat absorption, for use in producing a fat absorption enhancer, or for promoting fat absorption.

The use and methods of the invention may be used in both human and non-human mammals, and may also be used therapeutically or non-therapeuticly. "Non-therapeutic" as used herein means not including the operation, treatment or diagnosis of a human being (ie, medical treatment of a human being), specifically, it does not include a physician or a physician's instruction, Humans perform methods such as surgery, treatment or diagnosis.

The fat absorption enhancer and the fat absorption-enhancing composition of the present invention can be provided in the form of a pharmaceutical (for example, a pharmaceutical composition), a quasi-drug, a food, a feed, or the like, and can be carried out according to the method described below.

The present invention can be ingested or administered alone, or can be ingested or administered in combination with a general diet or the like. The fat absorption enhancer of the present invention, that is, the fat containing β-palmitic acid, has excellent digestive absorption to fat compared with the composition containing the oil and the like, and is particularly suitable for use in the nutrition of infants and young children. Replenish and improve nutritional status. Here, the infants and young children include infants and young children, and the details include infants, young children, and newborns, and more specifically include infants, young children, newborns, under-term infants, premature infants, and low birth weight infants. In the case of infants and young children, the milking period is the period when breast milk is the main source of nutrition. In human cases, it is generally less than 1 year old and is equivalent to the baby stage. Children refer to children from the general pre-primary school. Neonate refers to a child who is equivalent to a neonatal period. The neonatal period is the period of birth. The condition of a human is generally called the neonatal period within 4 weeks after birth.

In the present invention, for example, a fat-containing fat-absorbing composition (that is, a composition for promoting fat absorption), a nutritional supplement, and a milk for infants can be prepared by combining a fat containing β-palmitic acid with other raw materials. Use milk powder, etc. At this time, as described above, since the present invention is particularly suitable for nutritional supplementation and improvement of nutritional status of infants and young children, it is particularly suitable for use as a prepared milk powder for infants and young children.

The intake and the amount of the fat absorption enhancer of the present invention can be appropriately set in consideration of various factors such as the nutritional status, age, body weight, and symptoms of the subject and the subject. The intake and administration amount of the fat absorption enhancer of the present invention are not particularly limited. Further, the weight per unit package of the fat absorption enhancer of the present invention is not particularly limited, and may be, for example, in the range of 10 g or more and 500 g or less, more preferably 25 g or more and 250 g or less, and most preferably 50 g or more and 200 g or less. In addition, the unit package described above is not only a unit package of each bag, each box, and each container, but may be a unit package of the primary quantity contained in the items, or a unit package of one day. Moreover, a plurality of days, for example, a suitable intake and administration amount of one week may be packaged together, or a plurality of individual packagers may be included.

[Examples] Hereinafter, the present invention will be more specifically described on the basis of examples. And this embodiment does not limit the scope of the invention.

[Example 1] The influence of the difference in the binding ratio of the palmitic acid at the β-position on the absorption rate of palmitic acid was examined. Each of the oils having a low β-position binding ratio of palmitic acid, a medium-grade fat and oil, and a high fat-binding ratio were set as Comparative Example 1, Comparative Example 2, and Example 1. The feed composition of each group was shown in Table 1. Further, by adjusting the composition of the "sample oil" shown in Table 1, the β-bit binding ratio of palmitic acid in the samples taken by each group was adjusted. The composition of the main fatty acids in each group and the β-position binding ratio of palmitic acid are shown in Table 2. Further, the "β-position binding ratio" of palmitic acid means the ratio (mass ratio) of palmitic acid bound to the β position of the triglyceride relative to the total weight of palmitic acid.

Table 1: Composition of feed

Table 2: Composition of major fatty acids and beta binding ratio of palmitic acid

After 5 weeks old SD mice were domesticated for 1 week, 8 groups per group were divided into 3 groups (Comparative Example 1, Comparative Example 2, and Example 1), and the β-position binding ratio of the mixed palmitic acid alone was different. The feed of the oil (the aforementioned Tables 1, 2) was kept for one week. The feces of the last 3 days of the recovery experiment were provided as a lipid assay. Further, in the experiment, no significant difference between the groups was observed for the amount of the feed, the weight gain, and the dry weight of the feces of the mice (Table 3 shows the mean ± standard deviation).

Table 3: Feed intake, weight gain and dry weight of feces

The recovered feces were freeze-dried, crushed, and subjected to lipid extraction according to the method of Jeejeebhoy et al. (Clin. Biochem., 1970). The fatty acids of the extracted lipids were analyzed by the GC method and supplied to the total lipid mass analysis by gravimetric method. As a result, there was no significant difference in the amount of fatty acids taken, and the amount of palmitic acid excreted in the feces was significantly lower in Example 1 than in Comparative Example 1 and Comparative Example 2 ( FIG. 1 ). In addition, the total lipid mass in the feces was also a significant decrease in Example 1 (Fig. 2). From these results, it is known that the absorption of palmitic acid and total lipids is changed by the binding position with the triglyceride, and the greater the binding ratio at the β position, the smaller the excretion of palmitic acid and total lipids. It is clear that the absorption of palmitic acid and total lipids is improved.

[Example 2] In order to simulate the characteristics at the time of digesting after ingesting fats and oils, the following experiment was performed. The fats and oils of Example 2 and Comparative Example 3 were prepared. The main fatty acid compositions of Example 2 and Comparative Example 3 are shown in Table 4. The large difference between the fats of the examples and the comparative examples is only the ratio of binding to the β-position of palmitic acid, and the main fatty acid compositions are almost the same. Using the fats and oils of these Example 2 and Comparative Example 3, the artificial digestion reaction liquid was prepared according to the composition shown in Table 5. The concentration of the components in the artificial digestion reaction solution after preparation was 20 mg/mL of bile acid, 2.4 mg/mL of pancreatic enzyme, 19 mM of calcium chloride, 150 mM of sodium chloride, and 2.5% of fat. In addition, "Lipase from porcine pancreas (Type II) (Cat. No. L3126, manufactured by Sigma-Aldrich Co., Ltd.)" was used for the pancreatic enzyme system.

Table 4: Major fatty acid composition and β-binding ratio of palmitic acid

Table 5: Composition of artificial digestion reaction solution

The artificial digestion reaction solution described above was applied to a glass beaker having a volume of 50 mL for about Example 2 and Comparative Example 3, and placed in a water bath set at 37 ° C, and stirred and reacted with a magnetic stirrer. hour. An auto-titration device was used for the reaction, and 0.5 N sodium hydroxide solution was added dropwise to maintain the reaction solution at pH 7.0. The particle size and interface potential of the reaction solution were measured before and after the reaction. The particle size of the reaction solution was measured using a laser refracting and random particle size distribution measuring apparatus (Beckmancoulter, LS13320). The interface potential was measured using an interface potential measurement system (ELS-Z1, manufactured by Otsuka Electronics Co., Ltd.).

The measurement results are shown in Table 6. In Example 2 and Comparative Example 3, the artificial digestion reaction solution after the digestion reaction showed an increase in particle diameter. However, from the viewpoint of the particle diameter value, the particle diameter value of Example 2 was small, and as a result, Example 2 was considered to be preferable in absorbability. In the performance of the absolute value of the interface potential, both Example 2 and Comparative Example 3 increased after the digestion reaction. However, the amount of increase in Example 2 was small, and the absolute value thereof was smaller than that of Comparative Example 3 by the value of Example 2. The interface potential is an index of the surface charge of the particles. Since the larger the absolute value, the particles and the particles repel each other, and the state does not agglomerate and settles. In other words, in the oil of Comparative Example 3 in which the absolute value of the interface potential is large, since the particles are in a stable state, the surface of the intestinal epithelial cells is less likely to cause dissociation of fatty acids or triglycerides from the particles, and the absolute value of the interface potential is small. In the oil and fat of Example 2, since the particles are in an unstable state, the surface of the intestinal epithelial cells is liable to cause dissociation of fatty acids or triglycerides from the particles, and it is considered to be in a state of being easily absorbed.

Table 6: Particle size and interface potential measurement results

From the above results, it was found that the fats and oils containing palmitic acid having a high ratio of binding to the β-position at a high concentration were compared with the fats and oils containing palmitic acid having a high ratio of binding to the β-position at a low concentration, and it was found to have excellent fat-digesting properties. Therefore, a composition containing a fat or oil having a high ratio of binding to palmitic acid at a high concentration is used, and has an excellent digestion and absorption property against fat as compared with a composition containing a prior fat, and is considered to greatly contribute to, for example, a suckling child. Nutritional supplementation with elderly people and improved nutritional status.

[Fig. 1] In the animal experiment in which the ratio of palmitic acid to β-position was different, but the absorption rate of palmitic acid and total lipid was affected, Comparative Example 1, Comparative Example 2, Example 1 A chart showing the difference in acid emissions. Each group was implemented with n=8 and the results were expressed as an average. The vertical axis is the standard deviation. There was a meaningful difference between the different symbols (p<0.05). [Fig. 2] It is shown that in the animal experiment in which the ratio of palmitic acid to β-position was different, but the absorption rate of palmitic acid and total lipid was affected, the total amount of feces in each of Comparative Example 1, Comparative Example 2, and Example 1 was compared. A chart showing the difference in lipid excretion. Each group was implemented with n=8 and the results were expressed as an average. The vertical axis is the standard deviation. There was a meaningful difference between the different symbols (p<0.05).

Claims (8)

A fat absorption enhancer comprising a fat containing β-palmitic acid as an active ingredient. The fat absorption improving agent according to claim 1, wherein the ratio of palmitic acid in the fat to the total amount of palmitic acid is 54% or more and less than 98%. A use of a fat containing β-palmitic acid for producing a composition for promoting fat absorption, or for producing a fat absorption enhancer, for promoting fat absorption, or as a fat absorption enhancer. As used in claim 3, wherein the ratio of palmitic acid in the fat to the palmitic acid in the fat or oil is more than 54% and less than 98%. A method for promoting fat absorption, which comprises the steps of allowing a mammal to take in fat containing β-palmitic acid or administering it. A method for promoting fat absorption according to claim 5, wherein the ratio of the palmitic acid at the β position to the total amount of palmitic acid is less than 94%. A fat or oil containing β-palmitic acid, which is used for producing a composition for promoting fat absorption, or for producing a fat absorption enhancer, or for promoting fat absorption. The oil of claim 7, wherein the ratio of palmitic acid in the β-position relative to the total amount of palmitic acid is 54% or less and less than 98%.