TW201823446A - Microorganism having ability to control plant disease - Google Patents

Abstract

The present invention addresses the problem of providing novel microorganisms that impose little burden on the environment, have very little likelihood of leading to resistant pathogenic organisms, and have an exceptional ability to control a wider variety of plant diseases. In particular, present invention addresses the problem of providing novel microorganisms capable of exhibiting a control effect on not only filamentous fungal diseases but also bacterial diseases. These novel microorganisms are Pseudomonas azotoformans strain W-14-1 NITE BP-02371 or a mutant strain thereof and have a high control effect against various plant diseases including lettuce soft rot.

Description

Microorganisms capable of controlling plant diseases

The present invention relates to a microorganism having the ability to control plant diseases. This case claims priority from Japanese Patent Application No. 2016-232222 filed on November 30, 2016, and the contents are incorporated herein by reference.

As the main control method of plant diseases, the industry uses more chemical synthetic pesticides. However, there are fewer types of action points of chemically synthesized pesticides, and drug resistance of plant pathogens has become a problem in recent years due to repeated administration. Especially for bacterial plant diseases, only registration and use of inorganic and organic copper agents, Jiacimycin, streptomycin, oxytetracycline and other lesser types of antibiotics, and as synthetic antibacterial agents, only registration 1 、 Osulin acid is used. For anti-biomass and synthetic antibacterial agents, the performance of drug-resistant bacteria is more serious, and sometimes restrictions on use are set. In addition, inorganic and organic copper agents are likely to cause phytotoxicity to agricultural and horticultural crops, and the use time or crops are limited. Therefore, in order to overcome the problem of drug-resistant bacteria or phytotoxicity, as an alternative to or in combination with previous synthetic fungicides, the industry's attention to biological pesticides has increased. Compared with previous synthetic fungicides, biological pesticides have the advantages of less environmental pollution, coordination with the ecosystem, and excellent control effects. As a microbial pesticide used for the prevention and control of bacterial diseases in agricultural and horticultural crops, for example, Pseudomonas rhodesiae or a variant thereof having a capability of controlling plant diseases is known (Patent Document 1). In addition, an agricultural and horticultural bactericidal product containing Pseudomonas sp. Agent composition (Patent Document 2) and Patent Document 3 describe a spoilage disease (Pseudomonas cichorii) that is a bacterial disease of lettuce (Pseudomonas fluorescens) G7090 As strains of Pseudomonas azotoformans, F30A strain is described in Patent Document 4, S4 strain is active in rice seedling disease in Non-patent Document 1, and GC-B19 is described in Non-Patent Document 2. The strain is active against cucumber anthracnose. Non-Patent Document 3 (the strain is described in the literature as the name of Iizuka and Komagata F-3) describes NBRC12693 strain and Patent Documents 5 to 7 describe Pseudomonas azotoformans) as an example of bacteria. Among these nitrogen-producing Pseudomonas species, only S4 strain and GC-B19 strain are known as the strains capable of controlling plant diseases. However, with regard to these microbial pesticides, plant diseases that are generally targeted are limited to bacterial diseases or a very small part of filamentous fungal diseases, and almost no effect can be expected on other bacterial diseases and most filamentous fungal diseases. Therefore, it is desirable to develop a microbial pesticide that is effective against a wide variety of diseases, is practical as a pesticide, and has high activity. Prior Art Literature Patent Literature Patent Literature 1: WO2008 / 056653A Specification Patent Literature 2: Japanese Patent Laid-Open No. 11-302120 Patent Literature 3: Japanese Patent Laid-Open No. 2001-247423 Patent Literature 4: Japanese Patent Special Table 2013-514805 Patent Document 5: Japanese Patent Publication No. 2016-528893 Patent Literature 6: Japanese Patent Publication No. 2015-164429 Patent Literature 7: Japanese Patent Publication No. 2013-511276 Non-Patent Literature 1: "Journal of Biotechnology ", April 11, 2016, No. 227, p.25-26 Non-Patent Literature 2:" PHYTOPATHOLOGY ", (United States), January 29, 2014, Volume 104, No. 8, p.834- 842 Non-Patent Document 3: "Journal of the Agrochemical Society of Japan", July 23, 1963, Vol. 37, No. 3, p. 137-141

[Problems to be Solved by the Invention] The object of the present invention is to provide a novel microorganism which has a small load on the environment, has a low possibility of generating drug-resistant pathogenic bacteria, and has excellent control ability against a wide variety of plant diseases. In particular, the present invention provides a novel microorganism which is not only useful for filamentous fungal diseases, but also useful as a pesticide for both bacterial diseases and filamentous fungal diseases, and which can show a high control effect. [Technical means for solving problems] In order to solve the above problems, the present inventors collected microorganisms from various plants for cultivation and isolation, and searched for highly active microorganisms. As a result, microorganisms having a high control effect on various plant diseases represented by lettuce soft rot were found. Then, the mycological properties of the microorganism and the base sequence of the 16S rDNA gene were investigated. As a result, it was identified as a new strain of Pseudomonas azotoformans. That is, the present invention relates to the following inventions. (1) A nitrogen-producing Pseudomonas W-14-1 strain NITE BP-02371 or a variant thereof. (2) A method for preventing and controlling plant diseases, characterized in that the plant and / or the cultivation soil of the plant are treated by using a nitrogen producing Pseudomonas W-14-1 strain NITE BP-02371 or a mutant strain thereof. (3) A plant disease control agent, which contains Pseudomonas nitrogen-producing W-14-1 strain NITE BP-02371 or a variant thereof, and a surfactant. (4) The plant disease control agent according to (3), wherein the formulation ratio of the surfactant is 1 part by mass with respect to 1 part by mass of Pseudomonas nitrogen-producing W-14-1 strain NITE BP-02371 or a variant thereof. 0.01 to 30 parts by mass. [Effect of the invention] The microorganism of the present invention has a small load on the environment, and the possibility of generating drug-resistant pathogenic bacteria is extremely low, and it has excellent control ability against a variety of plant diseases for a longer time. Moreover, it can show the control effect on both a bacterial disease and a filamentous fungus disease.

<1> Microorganism of the present invention The microorganism of the present invention includes a Pseudomonas azotoformans W-14-1 strain having a capability of preventing plant diseases and a variant thereof, and the mutant strain is not artificial. Operation, obtained from the strain by natural mutation, or obtained from the strain by artificial mutation induction operation, and has the ability to control plant diseases. "Having the ability to control plant diseases" means that it has an antagonistic effect on the pathogenic bacteria of any plant disease. The microorganism of the present invention exerts an antagonizing effect on the pathogenic bacteria of plant diseases, thereby preventing or curing the plant diseases caused by the pathogenic bacteria, especially the effect of preventing plant diseases. "Preventing plant diseases" refers to treating the microorganisms of the present invention with pathogenic bacteria that are not infected with plant diseases, or plants that do not show signs of disease, or the soil in which they are cultivated. In addition, when the microorganisms are cultivated under the same suitable conditions, Compared with plants treated with the microorganisms of the present invention, plants treated with the microorganisms of the present invention have a lower degree of disease. "Healing plant diseases" refers to the case where plants infected with pathogenic bacteria of plant diseases and whose symptoms appear are treated under the same suitable conditions except for the treatment with the microorganisms of the present invention, and the microorganisms of the present invention are not treated. Compared to plants, the degree of disease in plants treated with the microorganisms of the present invention is lower. "Lower degree of disease" means, for example, that the incidence (or rate) is low and the control value is greater than zero. The control value is preferably a larger value, which is excellent if it is 30 or more, more excellent if it is 50 or more, and particularly excellent if it is 60 or 70 or more. Pseudomonas nitrogen-producing W-14-1 strain was deposited on October 12, 2016 under the international deposit number NITE BP-02371 and was internationally deposited at the Independent Administrative Corporation Product Evaluation Technology Base Agency Patent Microbial Deposit Center (NPMD) (Chiba Prefecture Room 122, 2-5-8 Kamakusa, Kisarazu City). The mycological properties of the P. aeruginosa W-14-1 strain (hereinafter referred to as "the microorganism") as the microorganism of the present invention are as follows. The microorganism is a Gram-negative bacillus, which does not form spores. The total length of the cells is 1.5-2.5 μm, and the full width is about 0.8 μm. Mobility is visible. Irregular colonies appear on standard agar medium and produce fluorescent pigments in King's B medium. No growth was seen at 41 ° C, positive enzyme activity was tested, OF medium was tested for oxidation, nitrate reduction was negative, indole production was negative, urease activity was negative, gelatin degradation was positive, β-galactosidase Activity was negative and β-glucosidase activity was negative. Moreover, in the LOPAT test, the production of levans was positive, the potato tuber spoilage was negative, the tobacco hypersensitivity reaction was negative, the oxidase activity was positive, and the degradation of arginine was positive. Starch microbial availability is negative. Regarding microbial availability of carbon compounds such as sugar and organic acids, D-glucose is positive, L-arabinose is positive, D-mannose is positive, D-mannitol is positive, and N- Acetyl-D-glucosamine was positive, maltose was negative, potassium gluconate was positive, n-decanoic acid was positive, adipic acid was negative, DL-malic acid was positive, sodium citrate was positive, and phenyl acetate It was negative, sucrose was positive, trehalose was positive, ribitol (lateral calendula) was positive, sorbitol was positive, butyric acid was negative, propionic acid was positive, and propylene glycol was negative. The measurement method and the like of each item are not particularly limited, and generally known methods can be used. For example, the method described in Patent Document 1 can be used. The microorganism can be obtained by culturing the bacterial cell after isolation using the sequence of the 16S rDNA gene (reference sequence number 3) and / or the above-mentioned mycological properties of the strain as indicators. The microorganism can be any one of the forms (e.g., dormant cells) displayed by the viable bacteria of P. aeruginosa W-14-1 represented by vegetative cells of P. aeruginosa strain W-14-1. Bacterial cells. (Cultivation method) The method for cultivating the microorganism is not particularly limited. For example, in the case of solid culture, a standard agar medium, a common agar medium, and a potato glucose agar medium may be used for standing culture at 20 to 35 ° C. As the method, in the case of liquid culture, a method of using various liquid culture media in which agar is removed from the above-mentioned agar culture medium, etc., and shaking culture at 20 to 35 ° C can be mentioned. (Plant diseases) This microorganism can be used to prevent plant diseases from bacteria belonging to absolutely aerobic bacteria, microaerobic bacteria, facultative anaerobic bacteria, and the like, and can be used to control bacteria from algae (Oomycetes (Oomycetes) )), Plant diseases of filamentous fungi such as Ascomycetes, Deuteromycetes, Basidiomycetes, Zygomycetes, etc. For example, the microorganism has the ability to control the following diseases: soft rot, rot disease, spot bacterial disease, leaf bacterial disease, ulcer disease, perforation bacterial disease, black spot bacterial disease, brown spot bacterial disease, stalk gangrene bacterial disease, gut Bacterial diseases, bacterial blight, bacterial wilt, bacterial blight, black rot, fire blight and other bacterial diseases; gray mold, gray star disease, sclerotinia, brown spot disease, black spot disease, spotted leaf disease , Black spot disease, early blight, ring rot, leaf mold, coal mold, scab, anthracnose, powdery mildew, rust, black spot, blight, exposed fungus and other filamentous fungal diseases. However, the microorganisms to be controlled are not limited to these. Examples of plant diseases (pathogenic bacteria) to be controlled are shown below. Sugar beet: brown spot (Cercospora beticola), black root disease (Aphanomyces cochlioides), root rot (Thanatephorus cucumeris), leaf rot ( Groundnut (Thanatephorus cucumeris) and other groundnuts: brown spot (Mycosphaerella arachidis), stain (Ascochyta sp.), Rust (Puccinia arachidis )), Rhizoctonia solani (Pythium debaryanum), Rust spot disease (Alternaria alternata), White silk disease (Sclerotium rolfsii), Black sclerosis ( Berkeley (Mycosphaerella berkeleyi)) and other cucumbers: powdery mildew (Sphaerotheca fuliginea), dew disease (Pseudoperonospora cubensis), blight (Mycosphaerella melonis) ), Stem rot (Fusarium oxysporum), sclerotinia (Sclerotinia sclerotiorum), gray mold (Botrytis cinerea), anthracnose (Colletotrichum orbiculare) ), Black star disease (Cladosporium cucumerin um)), brown spot disease (Corynespora cassiicola), sclerotinia blight (Pythium debaryanum, Rhizoctonia solani Kuhn), mycorrhizal fungus Rot (Phomopsis sp.), Spot bacteriosis (Pseudomonas syringae pv. Lechrymans) and other tomatoes: gray mold (Botrytis cinerea), leaf mold (Cladosporium fulvum), blight (Phytophthora infestans), half body blight (Verticillium albo-atrum, Verticillium dahliae), Eggplants such as powdery mildew (Oidium neolycopersici), rotella (Alternaria solani), coal mold (Pseudocercospora fuligena) and other eggplants: gray mold (gray Botrytis cinerea), black blight (Corynespora melongenae), powdery mildew (Erysiphe cichoracearum), coal mold (Mycovellosiella nattrassii), Sclerotinia (Sclerotinia sclerotiorum), half body wilt (Verticillium dahl iae)), brown spot disease (Phomopsis vexans) and other strawberries: gray mold (Botrytis cinerea), powdery mildew (Sphaerotheca humuli), anthracnose (anthracnose oxysporum) (Colletotrichum acutatum), Strawberry anthrax (Colletotrichum fragariae)), blight (Phytophthora cactorum), soft rot (Rhizopus stolonifer), chlorosis (Fusarium oxysporum) ), Onion (Verticillium dahliae) and other onions: gray rot (Botrytis allii), gray mold (Botrytis cinerea), white leaf blight ( Botrytis squamosa), exposed fungus (Peronospora destructor), white blight (Phytophthora porri) and other cabbages: rhizobium (Plasmodiophora brassicae), Soft rot (Erwinia carotovora), black rot (Xanthomonas campesrtis pv. Campestris), black spot bacterial disease (Pseudomonas syringae pv. maculicola), P. s. pv. alisalensis), Lu Disease (Peronospora parasitica), sclerotinia (Sclerotinia sclerotiorum), black coal disease (Alternaria brassicicola), gray mold (Botrytis cinerea) Lettuce: soft rot (Erwinia carotovora), spot bacterial disease (Xanthomonas axonopodis pv. Vitians) and other cabbage: soft rot (Erwinia carotovora) )) And other beans: Sclerotinia sclerotiorum (Sclerotinia sclerotiorum), gray mold (Botrytis cinerea), anthracnose (Colletotrichum lindemuthianum), keratosis (grey brown column silk) Phaeeosariopsis griseola) and other apples: powdery mildew (Podosphaera leucotricha), black spot disease (Venturia inaequalis), candidiasis (monilinia mali), Black spot disease (Mycosphaerella pomi), rot disease (Valsa mali), spotted leaf disease (Alternaria mali), red spot disease (Gymnosporangium yamadae) ) Rot disease (Botryosphaeria berengeriana), anthracnose (Glomerella cingulata, Colletotrichum acutatum), brown spot disease (Diplocarpon mali), Coal spot disease (Zygophiala jamaicensis), coal pollution (Gloeodes pomigena), purple feather disease (Helicobasidium mompa), gray mold disease (Gray mold (Botrytis cinerea)) and other plums: persimmons (Cladosporium carpophilum), gray mold (Botrytis cinerea), gray star disease (Monilinia mumecola), and other persimmons : Peach powdery mildew (Phyllactinia kakicola), anthracnose (Gloeosporium kaki), keratosis (Cercospora kaki) and other peaches: gray star disease (Peach gray star) Monilinia fructicola), black spot disease (Cladosporium carpophilum), Phytophthora spoilage (Phomopsis sp.), Perforated bacterial disease (Xanthomonas campestris Xanthomonas campestris pv. Pruni) and other almonds: gray star disease (Sclerotinia sclerotiorum) linia laxa)), spot disease (Stigmina carpophila), melanosis (Cladosporium carpophilum), leaf sclerosis (Polystigma rubrum), leaf spot disease (Alternaria alternata), anthracnose (Colletotrichum gloeospoides) and other cherries: gray star disease (Monilinia fructicola), anthracnose (Colletotrichum acutatum), black spot disease (Alternaria sp.), young fruit sclerotinia (Monilinia kusanoi) and other grapes: gray mold (Botrytis cinerea), white powder Disease (Uncinula necator), late rot (Glomerella cingulata, Colletotrichum acutatum), dew disease (Plasmopara viticola), black Pox (Elsinoe ampelina), Brown spot disease (Pseudocercospora vitis), Black rot (Guignardia bidwellii), White rot (Puccinia striiformis) (Coniella castaneicola)), rust (Phakopsora ampelopsidis) Pears: black spot disease (Venturia nashicola), red spot disease (Gymnosporangium asiaticum), black spot disease (Alternaria kikuchiana), ring pattern disease (pear Botryosphaeria berengeriana), powdery mildew (Phyllactinia mali), blight (Phomopsis fukushii), brown leaf spot (Stemphylium vesicarium )), Anthracnose (Glomerella cingulata) and other tea trees: gray blight (Pestalotiopsis longiseta, P. theae), anthracnose (anthrax Colletotrichum theae-sinensis)), net cake disease (Exobasidium reticulatum) and other citrus: scab (Elsinoe fawcettii), penicillium (Penicillium italicum), Green mold (Penicillium digitatum), gray mold (Botrytis cinerea), black spot (Diaporthe citri), ulcer disease (citrus canker Xanthomonas (Xanthomonas campestris pv. Citri)), powdery mildew (Oidium sp.)) and other wheat: powdery mildew (Blumeria graminis f. sp. tritici), scab (Gibberella zeae), red rust (Puccinia recondita) , Brown snow rot (Pythium iwayamai), red snow rot (Monographella nivalis), eye streak (Pseudocercosporella herpotrichoides), leaf blight (Septoria tritici), glume (Leptosphaeria nodorum), snow rot sclerotinia (Typhula incarnata), snow Sclerotinia sclerotiorum (Myriosclerotinia borealis), Rhizoctonia solani (Gaeumannomyces graminis), Ergot disease (Claviceps purpurea), Smut (wheat) Barley (Tilletia caries), Barley (Ustilago nuda), and other barley: leaf spot (Pyrenophora graminea), net spot (Pyrenophora graminea) (Pyrenophora teres)), moire (Rhynchosporium secalis), scattered black Disease (Ustilago tritici, U.nuda) and other rice: rice blast (Pyricularia oryzae), Rhizoctonia solani (Rhizoctonia solani )), Leggy disease (Gibberella fujikuroi), flax leaf blight (Cochliobolus miyabeanus), seedling blight (Pythium graminicola), white leaf blight (white leaf Xanthomonas oryzae), Bacterial bacterial disease (Burkholderia plantarii), Brown stripe disease (Acidovorax avenae), Rhizoctonia solani Burkholderia glumae), striped leaf blight (Cercospora oryzae), rice koji (Ustilaginoidea virens), brown rice (Alternaria alternata ), Curvularia intermedia), black-bellied rice (Alternaria padwickii), red rice (Epicoccum purpurascens) and other tobacco: Sclerotinia sclerotinia (Sclerotinia sclerotiorum)), powdery mildew (Erysiphe cichoracearum), blight ( Phytophthora nicotianae) and other tulips: Botrytis cinerea (Botrytis cinerea) and other sunflowers: Dew disease (Plasmopara halstedii), Sclerotinia sclerotinia (Sclerotinia sclerotiorum) ) And other zoysiagrass: Sclerotinia borealis (Sclerotinia borealis), Macrospot disease (Rhizoctonia solani), Brown spot disease (Rhizoctonia solani)), coin spot (Sclerotinia homoeocarpa), rice blast (Pyricularia sp.), red fever (Pythium aphanidermatum), anthracnose (Graminee anthracnose ( Colletotrichum graminicola)) and other orchard grasses: powdery mildew (Erysiphe graminis) and other soybeans: purple scar (Cercospora kikuchii), dew disease (Peronospora manshurica) , Stem blight (Phytophthora sojae), rust (Phakopsora pachyrhizi), sclerotinia (Sclerotinia sclerotiorum), anthracnose (Colletotrichum truncatum), gray Botrytis cinerea ytis cinerea), black pox (Elsinoe glycines), black spot (Diaporthe phaseolorum var. sojae), and other potatoes: blight (Phytophthora infestans) ), Early blight (Alternaria solani), black mole disease (Thanatephorus cucumeris), half body wilt (Verticillium albo-atrum), Verticillium dahliae (V. dahliae), V. nigrescens) and other bananas: banana wilt (Fusarium oxysporum), banana leaf spot (Mycosphaerella fijiensis), Rapeseeds such as Banana yellow stripe (M. musicola): Sclerotinia sclerotinia (Sclerotinia sclerotiorum), Root rot (Phoma lingam), Black spot (Brassica Coffee trees such as Alternaria brassicae): rust (Hemileia vastatrix), anthracnose (Colletotrichum coffeanum), brown eye disease (Cercospora coffeicola), etc. Sugarcane: brown rust (Puccinia melanocephala) and other corn: copper stain (Gloeocercospora sorghi), rust (Puccinia sorghi), southern rust (Puccinia polysora), smut (Ustilago maydis) ), Flax leaf blight (Cochliobolus heterostrophus), coal streak (Setosphaeria turcica) and other cotton: stand blight (Pythium sp.), Rust (Phakopsora gossypii), white spot disease (Mycosphaerella areola), anthracnose (Glomerella gossypii), etc. (applied plants) as the application object of this microorganism The plant is not particularly limited as long as it is a plant that the microorganism can exert its controlling ability. The microorganism can be used on cereals; vegetables; root vegetables; potato; fruit trees, tea trees, coffee, cocoa and other trees; forages; zoysia; cotton and other plants. Examples include cruciferae, solanaceae, melonae, liliaceae, legumes, asteraceae, Chenopodiaceae, Poaceae, Rosaceae, Dianthus, Primulaceae, Rutae, Grape, Kiwi, Diastaceae , Cress family, Convolvulaceae, or Araceae, which can be preferably listed as plants belonging to the Brassicaceae family such as cabbage, plants belonging to the Asteraceae family such as lettuce, plants belonging to the Solanaceae family such as potato, lemon, navel orange, etc. Plants of the family Rutaceae and pears belong to plants of the family Rosaceae. The microorganism can be applied to various parts of plants, such as leaves, stems, stalks, flowers, flower buds, fruits, seeds, germs, roots, tubers, tubers, shoots, cuttings, and the like. In addition, modified varieties of these plants-variants, cultivars, and further mutants, hybrids, and genetically modified organisms (GMOs) may be targeted. This microorganism can be used for seed treatment, seed tuber treatment, stem and leaf spray, soil application in order to prevent various diseases in agricultural and horticultural crops including flowers, zoysiagrass, and pasture. , Surface application, etc. <2> A plant disease control agent containing the microorganism The plant disease control agent containing the microorganism is not particularly limited as long as it contains a bacterial body of Pseudomonas nitrogen producing strain W-14-1 or a mutant strain thereof. Hereinafter, "the present control agent" means a control agent for plant diseases containing the microorganism. (Shape) The microorganism is not only the bacterial body itself, the suspension containing the bacterial body, the culture liquid containing the bacterial body, or the culture, concentrate, paste, dry matter, dilution, etc. Either form can be used for this control agent. (Prevention, cure) The present control agent can prevent or cure plant diseases caused by its pathogenic bacteria by its antagonistic effect on the pathogenic bacteria of plant diseases. The control agent can be used as a preventive agent for plant diseases or a therapeutic agent for plant diseases, and the effect as a preventive agent for plant diseases is particularly excellent. (Dilution concentration) The concentration of the microorganism contained in the control agent is not particularly limited as long as the effect of the present invention is not impaired. For example, when the concentration of the control agent is diluted to 1000 to 2000 times, it is preferably converted into Bacterial concentration, set to 1 × 10 ² ～ 1 × 10 ¹¹ cfu / ml, preferably 1 × 10 ⁴ ～ 1 × 10 ⁹ cfu / ml. (Additive) As long as the effect of the present invention is not impaired, the control agent may contain optional components in addition to the microorganism. The optional component is not particularly limited as long as the effect of the present invention is not impaired, and examples thereof include a carrier, a surfactant, a dispersant, and an adjuvant. It is particularly preferred to add at least a surfactant. Further, if necessary, an antioxidant, a colorant, a lubricant, an ultraviolet absorbent, an antistatic agent, a preservative, and the like are added. Furthermore, chemical pesticides such as fungicides, insecticides, herbicides, and growth regulators can also be mixed within a range that will not affect the microorganisms. In addition, even chemical pesticides such as fungicides, insecticides, herbicides, and growth regulators that affect the microorganism can be used by leaving a spray interval of several days. (Content of the microorganism) The content of the microorganism is not particularly limited, and is 0.001 to 99 parts by mass, preferably 0.01 to 80 parts by mass, and more preferably 0.1 to 70 parts by mass with respect to 100 parts by mass of the control agent. It is more preferably 1 to 50 parts by mass. (Additive: carrier) Examples of the carrier include inorganic salts such as calcium carbonate, potassium chloride, sodium sulfate, calcium sulfate, and ammonium sulfate; organic acids such as citric acid, malic acid, and stearic acid; and salts thereof; glucose , Lactose, sucrose and other sugars; alumina powder, silica gel, zeolite, hydroxyapatite, zirconium phosphate, titanium phosphate, titanium oxide, zinc oxide, hydrotalcite, kaolinite, montmorillonite, talc, clay, diatomaceous earth, Bentonite, white carbon, kaolin, vermiculite and other solid carriers. The content of the carrier is not particularly limited, and the formulation ratio of the carrier is 0.01 to 30 parts by mass, preferably 0.1 to 20 parts by mass, and more preferably 0.3 to 10 parts by mass with respect to 1 part by mass of the microorganism. (Additive: Surfactant or dispersant) As a surfactant (can also be used in the form of a dispersant), it is not particularly limited as long as it is a user in ordinary agricultural and horticultural preparations. Specifically, there are the following non- Ionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants. Examples of nonionic surfactants include sorbitan fatty acid esters (C12-18), POE (polyoxyethylene) sorbitan fatty acid esters (C12-18), and sucrose fatty acid esters. Sugar ester surfactants; POE fatty acid esters (C12-18), POE resin esters, POE fatty acid diesters (C12-18) and other fatty acid ester surfactants; POE alkyl ethers (C12-18), etc. Alcohol type surfactant; POE alkyl (C8-12) phenyl ether, POE dialkyl (C8-12) phenyl ether, POE alkyl (C8-12) phenyl ether formalin condensate and other alkylphenols Type surfactant; polyoxyethylene-polyoxypropylene block polymer; polyoxyethylene-polyoxypropylene block polymer type interface such as alkyl (C12-18) polyoxyethylene-polyoxypropylene block polymer ether Surfactants; POE alkylamines (C12-18), POE fatty acid ammonium amines (C12-18) and other alkylamine type surfactants; POE fatty acid diphenyl ether and other bisphenol type surfactants; POA benzylphenyl ( Polyaromatic ring-type surfactants such as phenylphenyl) ether, POA styrylphenyl (or phenylphenyl) ether; silicon-based and fluorine-based interfaces such as POE ether and ester-type silicon and fluorine-based surfactants Agent; POE castor oil, POE hydrogenated castor oil or the like type surfactant or the like. Examples of the anionic surfactant include alkyl sulfates (C12-18, Na, NH ₄ , Alkanolamine), POE alkyl ether sulfate (C12-18, Na, NH ₄ , Alkanolamine), POE alkyl phenyl ether sulfate (C12-18, NH ₄ , Alkanolamine), POE benzyl (or styryl) phenyl (or phenylphenyl) ether sulfate (Na, NH ₄ , Alkanolamine), polyoxyethylene, polyoxypropylene block polymer sulfate (Na, NH ₄ , Alkanolamine) and other sulfate-type surfactants; alkane (alkane) sulfonates (C12-22, Na, Ca, alkanolamine), AOS (C14-16, Na, alkanolamine), dioxane Sulfosulfosuccinate (C8-12, Na, Ca, Mg), alkylbenzene sulfonate (C12, Na, Ca, Mg, NH ₄ , Alkylamine, alkanol, amine, cyclohexylamine), mono- or dialkyl (C3-6) naphthalenesulfonate (Na, NH ₄ , Alkanolamine, Ca, Mg), naphthalenesulfonate-formalin condensate (Na, NH ₄ ), Alkyl (C8-12) diphenyl ether disulfonate (Na, NH ₄ ), Lignin sulfonate (Na, Ca), POE alkyl (C8-12) phenyl ether sulfonate (Na), POE alkyl (C12-18) ether sulfosuccinate half ester (Na), etc. Sulfonate type surfactant; carboxylic acid type fatty acid salt (C12 ~ 18, Na, K, NH ₄ , Alkanolamine), N-methyl-fatty acid inosinate (C12-18, Na), resinate (Na, K) and other POE alkyl (C12-18) ether phosphate (Na, alkanolamine ), POE mono- or dialkyl (C8-12) phenyl ether phosphate (Na, alkanolamine), POE benzyl (or styryl) phenyl (or phenylphenyl) ether phosphate (Na , Alkanolamine), polyoxyethylene-polyoxypropylene block polymer (Na, alkanolamine), phospholipids choline-phospholipids ethanolimine (lecithin), alkyl (C8-12) phosphate, etc. Phosphate-based surfactants, etc. Examples of the cationic surfactant include alkyltrimethylammonium chloride (C12-18), methyl-polyoxyethylene-alkylammonium chloride (C12-18), and alkyl-brominated N-formyl Ammonium surfactants such as pyridine (C12-18), mono- or dialkyl (C12-18) methylated ammonium chloride, alkyl (C12-18) pentamethylpropanediamine dichloride; alkyl Benzyl ammonium chloride type surfactants such as dimethyl benzyl ammonium chloride (C12-18), benzethonium chloride (octylphenoxyethoxyethyldimethylbenzyl ammonium chloride) and the like. Examples of the amphoteric surfactant include betaine-type surfactants such as dialkyl (C8-12) diaminoethyl betaine and alkyl (C12-18) dimethylbenzyl betaine; dialkyl (C8-12) glycine-type surfactants such as diaminoethylglycine, alkyl (C12-18) dimethylbenzylglycine, and the like. The surfactant and / or the dispersant may be used singly or in combination of two or more kinds. The content of the surfactant and / or dispersant is not particularly limited. The blending ratio of the surfactant is 0.01 to 30 parts by mass, preferably 0.1 to 20 parts by mass, and more preferably 0.3 to 1 part by mass of the microorganism. ~ 10 parts by mass. (Additive: Auxiliary) Examples of the auxiliary include carboxymethyl cellulose, polyethylene glycol, gum arabic, polyvinylpyrrolidone, and starch. (Formulation form) The form of the present control agent is not particularly limited, and it can be in a form that can be used by common agricultural and horticultural medicines, such as powder (DP, Dustable Powder), hydrating agent (WP, Wattable Powder), and emulsion (EC, Emulsifiable Concentrate). ), Suspending agent (FL, flowable), suspending agent (SC, Suspension Concentrate), water solvent (SP, Water Soluble Powder), granular hydrating agent (WG, Water Dispersible Granule), tablets (Tablet), granules (GR , Granule), SE (Suspo Emulsion, concentrated suspension), OD (Oil Dispersion, water-dispersible oil suspension), EW (Emulsion oil in water, water-based emulsion) and other forms. The preparation method of the preparation is not particularly limited, and a known preparation method can be adopted according to the dosage form. Examples of the formulation of the microorganisms are described below. (Formulation 1: Hydrating agent) 40 parts of the microorganism, 53 parts of diatomite, 4 parts of higher alcohol sulfate, and 3 parts of alkylnaphthalene sulfonate are uniformly mixed and pulverized to a fine size, thereby obtaining a hydrating agent of 40% of an active ingredient . (Preparation 2: Emulsion) 30 parts of xylene, 30 parts of xylene, 30 parts of dimethylformamide, and 7 parts of polyoxyethylene alkyl allyl ether were mixed and dissolved to obtain an emulsion of 30% of an active ingredient. (Preparation 3: Granules) 5 parts of the microorganism, 10 parts of talc, 38 parts of clay, 10 parts of bentonite, and 7 parts of sodium alkyl sulfate were uniformly mixed and pulverized to fineness, and granulated into granules having a diameter of 0.5 to 1.0 mm. A granule with an active ingredient of 5% was obtained. (Preparation 4: granules) 5 parts of the microorganism, 73 parts of bentonite, 20 parts of dioctylsulfosuccinate sodium salt, 1 part of potassium phosphate, and 1 part of potassium phosphate were sufficiently pulverized and mixed, and thoroughly mixed with water, and then granulated Dry to obtain 5% granules of active ingredient. (Formulation 5: Suspension agent) 10 parts of the microorganism, 4 parts of polyoxyethylene alkyl allyl ether, 4 parts of polycarboxylic acid sodium salt, 2 parts of glycerol, 10 parts of tricentrin, and 73.8 parts of water were mixed and wet-pulverized until The particle size becomes 3 micrometers or less, and a suspending agent with an active ingredient of 10% is obtained. (Plant diseases and applied plants of the present control agent) The plant diseases and applied plants that are the target of the present control agent are the same as the plant diseases and applied plants that are the target of the microorganism. <3> Method for controlling plant diseases using this control agent There is no particular limitation on the method for controlling plant diseases using this control agent as long as it is a method for treating plants and / or the cultivation soil of the plants using this control agent. The same manner as in the case of using ordinary chemical pesticides is appropriately selected depending on the kind of plant disease or the kind of plant to be applied. (Spraying method) For example, the plant body can be directly coated or sprayed by using the control agent, and the plant can be treated with the control agent, or the soil of cultivated plants can be mixed, sprayed, or sprayed with the control agent. (Cultivation soil of plants), etc., and the cultivation soil of plants is treated with the present control agent. In the case where the cultivation soil of plants is treated with the present control agent, plants can be grown after the soil is treated with the control agent, or the soil can be treated with the control agent after the plants are planted in the soil. In addition, as described in Japanese Patent Laid-Open No. 2001-302407, the control agent may be installed near the blowing port of a blowing device that blows the inside of the facility, and the pesticide is sprayed together with the air sent from the blowing port. Furthermore, for example, the plant can be coated, powder-sprayed, and the seeds or tubers of the plant can be impregnated with the present control agent, or treated by soaking the roots of the plant seedlings. (Soil spraying concentration) When the plant and / or the cultivation soil of the plant is treated with the control agent, the control agent may be used in its original state, or it may be diluted with an appropriate amount of water and used. The amount of the plant and / or the cultivation soil of the plant treated with the control agent varies according to the type of plant disease, the type of the applied plant, and the like, so it cannot be specified in general. In the case of spraying the soil, it can be converted. The bacterial cell concentration of the microorganism, usually set to 1 × 10 ² ～ 1 × 10 ¹¹ cfu / ml, preferably 1 × 10 ⁴ ～ 1 × 10 ⁹ cfu / ml range. In this control agent, as other ingredients, fungicides, insecticides, acaricides, nematicides, soil pesticides, plant regulators, synergists, fertilizers, soil improvers, and animals can be mixed or mixed. Feed, etc. By containing such other components, there may be cases where a synergistic effect is exerted. Specific examples of bactericides that can be used in combination with or in combination with the control agent are shown below. (1) Nucleic acid biosynthesis inhibitors: (a) RNA polymerase I inhibitors: benalaxyl, benalaxyl-M, furalaxyl, metalaxyl, Metalaxyl-M, oxadixyl, clozylacon, ofurace; (b) adenosine deaminase inhibitors: bupirimate, dimethirimol ), Ethirimol; (c) DNA / RNA synthesis inhibitors: hymexazol, octhilinone; (d) DNA topoisomerase II inhibitor: osolinic acid (oxolinic acid). (2) Mitosis inhibitors and cytokinesis inhibitors: (a) β-tubulin polymerization inhibitors: benomyl, carbendazim, chlorfenazole, fuberidazole ), Thiabendazole, thiophanate, thiophanate-methyl, diethofencarb, zoxamide, ethaboxam; (b) Cell division inhibitor: pencycuron; (c) Delocalized inhibitor of spectrin-like protein: fluopicolide. (3) Respiratory inhibitor: (a) Complex I NADH oxidoreductase inhibitor: diflumetorim, tolfenpyrad; (b) Complex II succinate dehydrogenase inhibitor: wheat rust Benodanil, flutolanil, mepronil, isofetamid, fluopyram, fenfuram, furmecyclox, Carboxin, oxycarboxin, thifluzamide, benzovindiflupyr, bixafen, fluxapyroxad, furametpyr ), Isopyrazam, penflufen, penthiopyrad, sedaxan, boscalid, pyraziflumid; (c) Complex III Ubiquinol oxidase Qo inhibitors: azoxystrobin, coumoxystrobin, coumethoxystrobin, enoxastrobin, flufenoxystrobin, fluoxoxybin (picoxystrobin), pyraoxystrobin, pyraclostrobin, pyrametostrobin, tricyclic Triclopyricarb, kresoxim-methyl, trifloxystrobin, dimoxystrobin, fenaminstrobin, metominostrobin, oxystrobin orysastrobin, famoxadone, fluoxastrobin, fenamidone, pyribencarb, mandestrobin; (d) complex III panthenol reductase Qi inhibitor: Sai Cyazofamid, amisulbrom; (e) oxidative phosphorylation decoupling agents: binapacryl, meptyldinocap, dinocap, fluazinam Fermizone; (f) Oxidative phosphorylation inhibitor (inhibitor of ATP synthase): fentin acetate, fentin chloride, fentin hydroxide); (g) ATP production inhibitor: silthiofam; (h) Complex III: Qx (unknown) inhibitor of cytochrome bc1 (ubiquinone reductase): ametoctradin . (4) Inhibitors of amino acids and protein synthesis (a) Inhibitors of methionine biosynthesis: andoprim, cyprodinil, mepanipyrim, and pirimethanil; (b) Inhibitors of protein synthesis: blasticidin-S, kasugamycin, kasugamycin hydrochloride, streptomycin, tereureus素 (oxytetracycline). (5) Signal transmission inhibitors: (a) Signal transmission inhibitors: quinoxyfen, proquinazid; (b) MAP-histidine kinase inhibitors of osmotic signal transmission: seed dressing (fenpiclonil), fludioxonil, chlozolinate, iprodione, procymidone, vinclozolin. (6) Inhibitors of lipid and cell membrane synthesis: (a) Phospholipid biosynthesis, methyltransferase inhibitors: edifenphos, iprobenfos, pyrazophos, Yazipu ( isoprothiolane); (b) Lipid peroxidants: biphenyl, chloroneb, dichloran, quintozene, tecnazene, degram Tolclofos-methyl, etridiazole; (c) agents acting on cell membranes: iodocarb, propamocarb, propacarb hydrochloride ( propamocarb-hydrochloride, propamocarb-fosetylate, prothiocarb; (d) microorganisms that disrupt the cell membrane of pathogenic bacteria: bacillus subtilis, bacillus subtilis strain QST713 QST713), bacillus subtilis strain FZB24, bacillus subtilis strain MBI600, bacillus subtilis strain D747, bacillus amyloliquefaciens (E) disrupting the cell membrane of the agent: Melaleuca alternifolia (tea tree) of extract (melaleuca altemifolia (tea tree) extract). (7) Inhibitor of sterol biosynthesis of cell membrane: (a) Demethylation inhibitor of C14 of sterol biosynthesis: triforine, pyrifenox, pyririxazole ), Fenarimol, flurprimidol, nuarimol, imazalil, imazalil-sulphate, oxpoconazole fumarate ), Pefurazoate, prochloraz, triflumizole, viniconazole, azaconazole, bitertanol, bromconazole ), Cyproconazole, diclobutrazol, difenoconazole, diniconazole, diniconazole-M, epoxyconazole, eticonazole (etaconazole), fenbuconazole, fluquinconazole, flusilazole, flutriafol, furconazole, furconazole-cis, Hexaconazole, imibenconazole, ipconazole, metconazo le), myclobutanil, penconazole, propiconazole, fluquinconazole, simeconazole, tebuconazole, tetraconazole, three Thai Triadimefon, triadimenol, triticonazole, prothioconazole, voriconazole, mefentrifluconazole; (b) Δ14 reductase and Δ8 → Δ7-isomerization of sterol biosynthesis Enzyme inhibitors: aldimorph, dodemorph, dodemorph acetate, fenpropimorph, tridemorph, fenpropidine , Piperalin, spiroxamine; (c) 3-keto reductase inhibitors at the C4 demethylation of the sterol biosynthesis system: fenhexamid, amine benzene Fenpyrazamine; (d) squalene epoxidase inhibitors of the sterol biosynthesis system: piributicarb, naftifin, terbinafine. (8) Inhibition of cell wall synthesis (a) Trehalase inhibitor: validamycin; (b) Chitin synthase inhibitor: polyoxins, polyoxorim ); (C) Cellulose synthase inhibitors: dimethomorph, flumorph, pirimorph, benthiavalicarb-isopropyl, iprovalicarb , Tolprocarb, valifenalate, mandipropamid. (9) Melanin biosynthesis inhibitor (a) Melanin biosynthesis reductase inhibitor: fthalide, pyroquilon, tricyclazole; (b) Dehydratase of melanin biosynthesis Inhibitors: carpropamid, dilocymet, fenoxanil. (10) Resistance inducers of host plants: (a) Agents acting on the salicylic acid synthesis pathway: acidified benzothiadiazole-S-methyl; (b) others: chlorpyrifos (probenazole), tiadinil, isotianil, laminarin, reynoutria sachalinensis extract. (11) Unknown agent: cymoxanil, fosetyl-aluminium, phosphoric acid (phosphate), tecloftalam, triazoxide, fluorine Flusulfamide, diclomezine, methasulfocarb, cyflufenamid, metrafenone, pyriofenone, dodine, donin Dodine free base, flutianil. (12) Agents with multiple action points: copper (copper salt), bordeaux mixture, copper hydroxide, copper naphthalate, copper oxide ( copper oxide), copper oxychloride, copper sulfate, sulfur, sulfur products, calcium polysulfide, ferbam, zinc manganese Mancozeb, maneb, mancopper, metiram, polycarbamate, methyl zinc propineb, thiram, Zineb, ziram, captan, captafol, folpet, chlorothalonil, dichlofluanid, formazan Tolylfluanid, guazatine, iminoctadine triacetate, iminoctadine trialbesilate, anilazine, dithianon, Quinomethionate, fluoroimide. (13) Other agents: DBEDC, fluorofolpet, guazatin acetate, bis (8-quinolinolato) copper (II), general Propamidine, chloropicrin, cyprofuram, agrobacterium, bethoxazin, diphenylamine, methyl isothiocyanate (MITC) (methyl isothiocyanate, mildew-mycin, capsaicin, cufraneb, cyprosulfamide, dazomet, debacarb, dichlorophenol (dichlorophen), flumetover, calcium triethyl phosphonate (fosetyl-calcium), sodium triethyl phosphonate (fosetyl-sodium), irumamycin, natamycin, nitrothal isopropyl , Oxamocarb, pyrrolnitrin, tefufloquin, tolnifanide, zarilamide, algophase, amicarthiazol, fluorothiazolyl-pyrone oxathiapiprolin), metiram zinc, benthiazole, trichlamide, uniconazole ( uniconazole), mildew-mycin, oxyfenthiin, picarbutrazox, fenpicoxamid, dichlobentiazox, quinofumelin, thiuram, ambam, Agrobacterium radiata ( agrobacterium radiobacter, coniothyrium minitans, pseudodomonas fluorescens, pseudodomonas rhodesiae, talaromyces flavus, trichoderma atroviride Erwinia carotovora subsp. carotovora), bacillus simplex, variovorax paradoxus, lactobacillus plantarum. Specific examples of insecticides, acaricides, nematicides, soil insecticides, insect repellents, and the like that can be used in combination with or in combination with the control agent are disclosed below. (1) Acetylcholinesterase inhibitor: (a) Carbamates: alanycarb, aldicarb, bendiocarb, benfuracarb, Butocarboxim, butoxycarboxim, carbaryl, carbofuran, carbosulfan, ethiofencarb, butyl carbamazepine (butylcarboxim) fenobucarb, formetanate, furathiocarb, isoprocarb, methiocarb, metomyl, oxamyl, pirimicarb ), Propoxur, thiodicarb, thiofanox, triazamate, trimethacarb, XMC, xylycarb, fenothiocarb ), MIPC, MPMC, MTMC, aldoxycarb, aliyxycarb, aminocarb, bufencarb, cloethocarb, metam-carb sodium), promecarb; (b) organophosphorus: acephate, azamethiphos, azonphos-ethyl, azonphos-methyl Cadusafos, chlorethoxyfos, chlorfenvinphos, chlormephos, chlorpyrifos, chlorpyrifos-methyl, coumaphos, cyanide Cyanophos, demeton-S-methyl, diazinon, dichlorvos / DDVP, dicrotophos, dimethoate, methyl poisonous insects Dimethylvinphos, disulfoton, EPN (ethyl para-nitro-phenyl), ethion, ethoprophos, famphur, fenfluxon (fenamiphos), fenitrothion, fenthion, fosthiazate, heptenophos, imicyafos, isofenphos, hydrofenthion (isocarbophos), isoxathion, marathion, mecarbam, methamidophos, methidathion, mevinphos, monocrotophos, Neli Pine (naled), omethoate, oxydemeton-methyl, parathion, methylparazon (parathion-methyl), phenthoate, phorate, phosalone, phosmet, phosphamidon, phoxim, arteson (pirimiphos-methyl), profenofos, propetamphos, prothiofos, pyraclofos, pyridaphenthion, quinalphos, quinol Sulfotep, tebupirimfos, temephos, terbufos, tetrachlorvinphos, thiometon, triazophos, triclosan (trichlorfon), vamidothion, bromophos-ethyl, BRP, carbophenothion, cyanofenphos, CYAP (cyanophos), demeton -S-methyl sulphone, dialifos, dichlofenthion, dioxabenzofos, etrimfos, fensulfothion, flupyrazofos ), Fonofos, formothion, fosmethilan, isazofos, Jodfenphos, metharifos, pirimiphos-ethyl, phosphocarb, propaphos, prothoate, sulprofos ). (2) GABA-gated chloride channel antagonists: acetoprole, chlordane, endosulfan, ethiprole, fipronil, fipronil Pyrafluprole, pyriprole, camphechlor, heptachlor, dienochlor. (3) Sodium channel regulators: anrinathrin, d-cis-trans allethrin, d-trans allethrin, bifennine ( bifenthrin), bioallethrin, bioailethrin s-cyclopentyl isomer, bioresmethrin, cycloprothrin , Cyfluthrin, β-cyfluthrin, cyhalothrin, λ-cyhalothrin, γ-cyhalothrin, cypermethrin ), Α-cypermethrin, β-cypermethrin, θ-cypermethrin, ζ-cypermethrin, azephenine [(1R ) -Trans isomer] (cyphenothrin [(1R) -trans isomer]), deltamethrin, Yifanin [(EZ)-(1R) -isomer] (Empenthrin [(EZ)- (1R) -Isomer]), esfenvalerate, etofenprox, fenpropathrin, fenvalerate, flucythrinate, flumethrin , Τ-fluvalinate, halfenp rox), imiprothrin, kadethrin, permethrin, phentermin [(1R) -trans isomer] (phenothrin [(1R) -trans isomer]), Prallethrin, pyrethrum, resmethrin, silafluofen, tefluthrin, [tetramethrin] (tetramethrin) [(1R) -isomer]), tralomethrin, transfluthrin, allethrin, pyrethrins, pyrethrin I, pyrethrin Sperm II (pyrethrin II), profluthrin, dimefluthrin, bioethanomethrin, biopermethrin, transpermethrin ), Fenfluthrin, fenpirithrin, flubrocythrinate, flufenprox, metofluthrin, protrifenbute, Antipyrethrin, terallethrin. (4) Nicotinic acetylcholine receptor agonists: acetamiprid, clothianidin, dinotefuran, imidacloprid, nitenpyram ), Nithiazine, thiacloprid, thiamethoxam, sulfoxaflor, nicotine, flupyradifurone. (5) Nicotinic Acetylcholine Receptor Ectopic Modulators: spinortoram, spinosad. (6) Chloride channel activators: abamectin, emamectine-benzoate, lepimectin, milbemectin, ivermectin, slack Seramectin, doramectin, eprinomectin, moxidectin, milbemycin, milbemycin oxime, nemectin (nemadectin). (7) Juvenile hormone substances: hydroprene, kinoprene, metoprene, fenoxycarb, pyriproxyfen, fenprofen (diofenolan), efofenonane, triprene. (8) Other non-specific inhibitors: methyl bromide, chloropicrin, sulfuryl fluoride, borax, and tartar emetic. (9) Homopteran selective feeding inhibitors: flonicamid, pymetrozine, and pyrifluquinazon. (10) Mite growth inhibitors: clofentezine, diflovidazin, hexythiazox, etoxazole. (11) Insect endometrial disrupting agent from microorganisms: bacillus thuringiensis subsp. israelensi), bacillus sphaericus, bacillus thuringiensis subsp. aizawai), Sully Kustak subspecies (bacillus thuringtensis subsp. kurstaki), thuringi subsp.bacillus thuringiensis subsp. tenebrionis), Bt crop proteins: Cry1Ab, Cry1Ac, Cry1Fa, Cry1A. 105. Cry2Ab, Vip3A, mCry3A, Cry3Ab, Cry3Bb, Cry34Ab1 / Cry35Ab1. (12) Inhibitors of mitochondrial ATP biosynthesis: diafenthiuron, azocyclotin, cyhexatin, fenbutatin oxide, propargite, demisite (tetradifon). (13) Oxidative phosphorylation and decoupling agents: chlorfenapyr, sulfuramid, DNOC (Dinitro-o-cresol, dinitro-o-cresol), binapacryl, and big mite (dinobuton), white powder gram (dinocap). (14) Nicotinic acetylcholine receptor channel blockers: bensultap, cartap hydrochloride, nereistozin, thiosultap-sodium, and thiosulfan (thiocyclam). (15) Chitin synthesis inhibitors: bistrifluron, chlorfluazuron, diflubenzuron, flucycloxuron, flufenoxuron, and hexafluron ( hexaflumuron, lufenuron, novaluron, noviflumuron, teflubenzuron, triflumuron, buprofezin, fluazuron ). (16) Diptera peeling disturbing agent: cyromazine. (17) Peeling hormone receptor agonists: chromafenozide, halofenozide, methoxyfenozide, tebufenozide. (18) Octopamine receptor agonists: amitraz, demiditraz, chlordimeform. (19) Mitochondrial electron transport system complex III inhibitors: acequinocyl, fluacrypyrim, hydramethylnon. (20) Mitochondrial electron transport system complex I inhibitors: fenazaquin, fenpyroximate, pyrimidifen, pyridaben, tebufenpyrad, defenfen Tolfenpyrad, rotenone. (21) Potential-dependent sodium channel blockers: indoxacarb, metaflumizone. (22) Acetyl coenzyme A carboxylase inhibitor: spirodiclofen, spiromesifen, and spirotetramat. (23) Mitochondrial electron transport system complex IV inhibitor: aluminum phosphide, calcium phosphide, phosphine, zinc phosphide, cyanide. (24) Mitochondrial electron transport system complex II inhibitors: cyenopyrafen, cyflumetofen, pyflubumide. (25) Ryanodine receptor modulator: chlorantraniliprole, cyantraniliprole, flubendiamide, cyclaniliprole, tetraniliprole . (26) Mixed-function oxidase inhibitor compound: piperonyl butoxide. (27) Spider toxin receptor acting drugs: depsipeptide, cyclodepsipeptide, 24-membered cyclodepsipeptide, and emodepside. (28) Other agents (the mechanism of action is unknown): azadirachtin, benzoximate, bifenazate, bromopropylate, quinomethionate, cryolite ), Dicofol, pyridalyl, benclothiaz, sulfur, amidoflumet, 1,3-dichloropropene ), DCIP (2,6-dichlorophenol indophenol), phenisobromolate, benzomate, metaldehyde, chlorobenzilate , Clothiazoben, dicyclanil, fenoxacrim, fentrifanil, flubenzimine, fluphenazine, attractants Gossyplure, japonilure, metoxadiazone, oil, potassium oleate, tetrasul, triarathene , Afidopyropen, flometoquin, flufiprole, fluensulfone, meperfluthrin, tefluthrin (te tramethylfluthrin), brolopyril, tralopyril, dimefluthrin, methylneodecanamide, fluralaner, afoxolaner, fluxametamide, 5- [5- (3,5-dichlorobenzene) ) -5-trifluoromethyl-4,5-dihydroisoxazol-3-yl] -2- (1H-1,2,4-triazol-1-yl) benzonitrile (CAS: 943137 -49-3) (5- [5- (3,5-dichlorophenyl) -5-trifluoromethyl-4,5-dihydroisoxazole-3-yl] -2- (1H-1,2,4-triazole-1-yl ) benzonitrile (CAS: 943137-49-3)), bromfenidil (broflanilide), other diamines, steinernema carpocapsae, steinernema glaseri , Invading pasteuria penetrans, paecilomyces tenuipes, paecilomyces fumosoroseus, beauveria bassiana, beauveria brongniartii, Metarhizium anisopliae, verticillium lecanii. (29) Insect repellent: (a) Benzimidazoles: fenbendazole, albendazole, trilabendazole, oxibendazole, tolda Mebendazole, oxfendazole, parbendazole, flubenzazole, febantel, netobimin, more Thiophanate, thiabendazole, cambendazole; (b) salicylidene aniline: closantel, oxyclozanide, ranifanide (rafoxanide), niclosamide; (c) Substituted phenols: nitroxinil, nitroscanate; (d) Pyrimidines: pyrantel, Morande (morantel); (e) imidazothiazole series: levamisole, tetramisole; (f) tetrahydropyrimidine pyrimidine series: praziquantel, epsiprantel; (g ) Other insect repellents: cyclodiene, ryania, closulon, metronidazole, demiditraz Piperazine, diethylcarbamazine, dichlorophen, monepantel, tribendimidine, amidantel, thiacetalsamide , Melarsomine, arsenamide. Specific examples of plant regulators which can be used in combination with or in combination with the control agent are disclosed below. Abscisic acid, kinetin, Benzylaminopurine, 1,3-diphenylurea, forchlorfenuron, thidiazuron , Chlorfenuron, dihydrozeatin, gibberellin A, gibberellin A4, gibberellin A7, gibberellin A3 ), 1-methylcyclopropane, 1-methylcyclopropane, N-acetylaminoethoxyvinylglycine (N-acetylaminoethoxyvinyl glycine (aviglycine)), amine Aminooxyacetate, silver nitrate, cobalt chloride, IAA, 4-CPA, cloprop, 2,4-D, MCPB, 3-indole butyric acid (indole- 3-butyrate), 2,4-dichlorprop, phenothiol, 1-naphthyl acetamide, etychlozate, cloxyfonac Maleic acid hydrazide, maleic acid hydrazide, 2,3,5-triiodobenzoic acid, salicylic acid, methyl salicylate ), (-)- (-)-Jasmonic acid, methyl jasmonate, (+)-strigol, (+)-deoxystranol ((+)- deoxystrigol), (+)-orobanchol, (+)-sorgolactone, 4-oxo-4- (2-phenylethyl) amine 4-oxo-4- (2-phenylethyl) aminobutyric acid, ethephon, chlormequat, mepiquat chloride, benzyl adenine (benzyladenine), 5 -5-amino levulinic acid, daminozide. (Number of spraying times) The number of spraying times of the control agent can be appropriately selected according to the type of plant disease, the type of applied plant, and the degree of the disease. (Use) The control agent can be mainly used in agriculture, and can also be used in other than agriculture. Examples of agricultural uses include: seed treatment, seed potato disinfection, spraying on soil of rice fields, fields, etc., spraying on vegetables, fruits, or rice and other plants, and other uses for agricultural use, such as bathrooms, living rooms, etc. Antifungal agent on the wall, water quality improver for storage tank, swimming pool, cooling tower, etc., sludge treatment agent for sludge, etc. (Spraying method) The control agent can be used without particular limitation on the spraying method, and can be exemplified: a method of spraying diluted with water and spraying the method; a method of spraying the solid agent directly into the soil; Ways to make an effect. Hereinafter, the present invention will be described more specifically with reference to examples, but the technical scope of the present invention is not limited to these examples. [Example] [Example 1] Isolation and identification of bacteria 1. Isolation of candidate strains and selection of strains with the ability to control plant diseases. The leaves of cabbage collected in farmland are homogenized in a small amount of sterilized water, and the obtained suspension is smeared on a standard agar medium (casein-based peptone 0. 5% (w / v), yeast extract 0. 25% (w / v), glucose 0. 1% (w / v), agar 1. 5% (w / v), pH value after sterilization 6. 9 ～ 7. 1) (Nissui Pharmaceutical Co., Ltd.) and culture at 25 ° C for 2 days. A plurality of growing single microbial colonies were collected, and each was regarded as a candidate strain. With regard to the candidate strains thus collected, Test Example 1 was used to select several strains having a control effect on the pathogenic bacteria (Erwinia carotovora) of lettuce soft rot. With regard to the selected strains, and further in the farmland test of Test Example 2, the strains having a control effect on the soft rot of cabbage were selected. Regarding the finally selected strain, the control value in Test Example 1 was 86. 7, the control value in Test Example 2 is 93. 8. This selected strain was named W-14-1 strain. [Experiment 1] Control effect of lettuce soft rot (bacterial disease) (indoor test) Put a standard liquid culture medium (yeast extract 0. 25%, peptone 0. 5%, glucose 0. 1%, pH value 7. 0) 150 ml, heat sterilization. Cultures inoculated for testing 1 ml was cultured in a reciprocating shaker at 30 ° C and 100 rpm for 3 days. The obtained culture solution was centrifuged to remove the supernatant, and the precipitate was washed with water. The centrifuge was centrifuged again to remove the supernatant, and the precipitate was washed with water. In this way, a bacterial cell suspension was prepared. Next, the bacterial cell suspension was diluted with water to prepare a treatment solution (A600 (absorbance at a wavelength of 600 nm) = 0. 1). Spray the treatment solution to the incision of the leaves of the middle part of the lettuce. Each lettuce sprayed was cultured under humidified conditions at 28 ° C. for 5 hours, and then the lettuce soft rot pathogen (Erwinia carotovora) in water suspension (A600 = 0. 05) Inoculate the cuts of each lettuce. After inoculation, the cells were cultured at 28 ° C for 4 days, and then the degree of disease was investigated according to the following criteria, and the incidence and control value were calculated based on the following formulae 1 and 2. Criterion 0: No lesions Criterion 1: 10% of lesions less than midribs Criterion 2: Lesions less than 10-50% of midribs Criteria 3: Lesions less than 50 to 75% of midribs Criteria 4: Lesions exceeding midribs 75% (Formula 1) Incidence rate = ((1 × n1 + 2 × n2 ++ 3 × n3 + 4 × n4) / (4 × total number of survey leaves)) × 100 n1 ～ n4 are the number of leaves corresponding to each of the benchmarks 1 ～ 4 ( Formula 2) Control value = (1-(incidence rate in treated area / incidence rate in non-treated area)) × 100 [Test Example 2] Control effect test of soft rot (bacterial disease) in cabbage (field test) 1 A bacterial cell suspension of the culture to be tested was prepared in the same manner. Next, the bacterial cell suspension was diluted with water to prepare a treatment solution (A600 = 0. 1). The treatment liquid was sprayed on the cabbage transplanted on the farmland and treated. The spraying treatment was performed twice at intervals of one week. In addition, the pathogenic fungus of soft rot of Chinese cabbage (Owenia soft rot) was inoculated with an aqueous suspension (A600 = 0. 0) on the day of the first spraying treatment. 1). One week after the second spraying, the degree of disease incidence was investigated according to the following criteria, and the diseased plant rate, disease incidence, and control value were calculated based on the following formulas 3 and 4. Criterion 0: Non-onset Criterion 1: Part of the outer lobe can be seen Criterion 2: The outer lobe and part of the nodule can be seen Criterion 3: Most of the nodule can be seen (formula 3) Incidence rate = ((1 × n1 + 2 × n2 + 3 × n3) / (3 × total number of plants surveyed)) × 100 n1 ～ n3 are the number of plants corresponding to each of the benchmarks 1 ～ 3 (Equation 4) Control value = (1— (Incitation rate in treatment area / no treatment Area incidence)) × 100 2. Analysis of 16S rDNA gene In order to analyze the nucleotide sequence of 16S rRNA gene for W-14-1 strain, an attempt was made to amplify 16S rRNA gene from W-14-1 strain. Specifically, first, genomic DNA was isolated from the W-14-1 strain according to a conventional method. Using the obtained genomic DNA as a template, PCR (polymerase chain reaction) amplification was performed using the 27F primer (sequence number 1) and 1510R primer (sequence number 2) often used in 16S rRNA gene amplification. The sequence of the obtained PCR product was determined, and the nucleotide sequence was read. Furthermore, the nucleotide sequence of the 16S rRNA gene is determined based on the obtained nucleotide sequence information. The nucleotide sequence of the 16S rRNA gene of the W-14-1 strain is shown in SEQ ID NO: 3. Based on the nucleotide sequence of the 16S rRNA gene of the W-14-1 strain, BLAST (basic local alignment search tool) was used to perform homology search. In addition, the nucleotide sequence of the 16S rRNA gene of W-14-1 strain was analyzed using ClustalX, and the analysis results obtained were processed using Tree View to prepare a phylogenetic tree related to W-14-1 strain (Figure 1) ). With this phylogenetic tree, the taxonomic location of the W-14-1 strain became clear. As a result, it was determined that the W-14-1 strain belongs to a group of bacteria belonging to the Pseudomonas fluorescens group, and is further included in Pseudomonas gainerii, Pseudomonas lebanonii, Pseudomonas xanthomonas, and M. pseudomonas A clade group consisting of Pseudomonas and Nitrogen-producing bacteria. Among them, it has high homology with P. nitrogen producing. 3. The analysis of mycological properties will be described above 2. The standard analysis of the 16S rRNA gene sequence is considered to be the standard strains of Pseudomonas gaernerii, Pseudomonas lebanon, Pseudomonas xanthomonas, Pseudomonas moldy and Pseudomonas nitrogen producing The colony morphology was compared. As a result, it was suggested that the morphological characteristics of the W-14-1 strain were particularly consistent with those of Pseudomonas nitrogen-producing or Pseudomonas lebanonum, which were either. In order to determine the type of W-14-1 strain, more detailed mycological properties of W-14-1 strain, Pseudomonas nitrogen-producing and Pseudomonas lebanonum were investigated and compared according to the specific methods described below. First, the mycological properties of W-14-1 strain are as follows. Gram-negative bacilli do not form spores, and the full length of the cells is 1. 5 ～ 2. 5 μm with a full width of 0. About 8 μm, movement is visible. Irregular colonies appear on standard agar medium and produce fluorescent pigments in King's B medium. No growth was seen at 41 ° C, positive enzyme activity was tested, OF medium was tested for oxidation, nitrate reduction was negative, indole production was negative, urease activity was negative, gelatin degradation was positive, β-galactosidase Activity was negative and β-glucosidase activity was negative. Moreover, in the LOPAT test, the production of levans was positive, the potato tuber spoilage was negative, the tobacco hypersensitivity reaction was negative, the oxidase activity was positive, and the degradation of arginine was positive. Starch microbial availability is negative. Regarding microbial availability of carbon compounds such as sugar and organic acids, D-glucose is positive, L-arabinose is positive, D-mannose is positive, D-mannitol is positive, and N- Acetyl-D-glucosamine was positive, maltose was negative, potassium gluconate was positive, n-decanoic acid was positive, adipic acid was negative, DL-malic acid was positive, sodium citrate was positive, and phenyl acetate It was negative, sucrose was positive, trehalose was positive, ribitol (lateral calendula) was positive, sorbitol was positive, butyric acid was negative, propionic acid was positive, and propylene glycol was negative. The results are shown in Table 1. [Table 1] In the items in Table 1, for the reduction of nitrate, indole production, OF medium test, degradation of arginine, urease activity, degradation of gelatin, β-galactosidase activity, β-glucosidase activity, D- The microbial availability of glucose, the microbial availability of L-arabinose, the microbial availability of D-mannose, the microbial availability of D-mannitol, the microbial availability of N-acetamyl-D-glucosamine, Maltose microbial availability, potassium gluconate microbial availability, n-decanoic acid microbial availability, adipic acid microbial availability, DL-malic acid microbial availability, sodium citrate microbial availability, phenyl acetate The microbial availability was tested using API20NE (manufactured by Nippon bioMerieux Co., Ltd.) in accordance with the attached operating instructions. The oxidase activity was tested using Oxidase Reagent (manufactured by Nippon bioMerieux Co., Ltd.), and the catalase activity was tested using ID color Catalase (manufactured by Nippon bioMerieux Co., Ltd.). The Jin B medium used in the Jin B medium test was performed using a Jin B medium manufactured by Rongken Chemical Co., Ltd. Gram staining was performed using Color Gram2 (manufactured by Nippon bioMerieux Co., Ltd.). Regarding the levans production test, the strain was streaked and transplanted on a plate of 5% sucrose plus ordinary agar medium, and cultured at 25 ° C for 3 days, and the white sticky bumps were formed into larger dome colonies. Judged as positive. Potato tuber putrefaction test, tobacco oversensitivity test, starch microbial availability test, and microbial availability of carbon compounds such as sugar or organic acids can be measured by known methods. For example, the method described in Patent Document 1 can be used. In order to understand what the W-14-1 strain is, Pseudomonas aeruginosa and Pseudomonas lebanonum, Pseudomonas aeruginosa strain NBRC12693 and Pseudomonas lebanonum CIP105460 are known as standard strains. Tests for items in Table 1. The results are shown in Table 1. In addition, it is known that Pseudomonas fluorescens, which is a two closely related species, exerts pathogenicity on tomato and causes tomato stem gangrene bacterial disease. In order to investigate whether the W-14-1 strain has such pathogenicity on tomato, The tests described below were performed. The W-14-1 strain was cultured in a liquid medium to A600 (absorbance at a wavelength of 600 nm) to 1.0, and the culture solution was sprayed and inoculated to the main stem and petiole portion centered on the petiole base of the tomato seedling. After 7 days of cultivation, the seedlings were observed. As a result, no symptoms of tomato stem gangrene bacterial disease were found. After 14 days of cultivation and 21 days of cultivation, seedlings were also observed, and similarly no symptoms of tomato stem gangrene bacterial disease were seen. In addition, after the tomato seedlings were damaged, the above-mentioned culture solution of W-14-1 strain was inoculated into this part and the same test was performed. Similarly, no symptoms of tomato stem gangrene bacterial disease were seen. Furthermore, the same test was performed by injecting the above-mentioned culture solution of the W-14-1 strain into a tomato seedling inoculated with a syringe, and similarly no symptoms of tomato stem gangrene bacterial disease were observed. According to the results in Table 1, the different aspects of the W-14-1 strain and the P. lebanese CIP105460 strain are the morphological characteristics of the side, periphery, and surface of the colony, the reducing ability of nitrate, the degrading power of gelatin, and the Degradability, microbial availability of sucrose, microbial availability of propionic acid, and microbial availability of propylene glycol. On the other hand, the W-14-1 strain differs from the nitrogen-producing Pseudomonas NBRC12693 strain only in the reducing ability of nitrate, the degradability of gelatin, the microbial availability of adipic acid, and the microbial availability of propylene glycol. . Based on the above results, the W-14-1 strain is more homologous to Pseudomonas nitrogen-producing than Pseudomonas lebanonus, and thus the W-14-1 strain was identified as P. nitrogen-producing. 4. Preparation of bacterial suspension In a 300 ml volume Erlenmeyer flask, put 150 ml of standard liquid culture medium (yeast extract 0.25%, peptone 0.5%, glucose 0.1%, pH 7.0) and heat sterilize. Inoculate 0.1 ml of a preculture of P. aeruginosa W-14-1 strain (NITE BP-02371), and culture in a reciprocating shaker at 30 ° C and 100 rpm for 3 days. The obtained culture solution was centrifuged to remove the supernatant, and the precipitate was washed with water. The centrifuge was centrifuged again to remove the supernatant, and the precipitate was washed with water. In this manner, a bacterial cell suspension of P. nitrogen-producing strain W-14-1 (hereinafter, referred to as a "cell suspension of W-14-1 strain") was prepared. 5. Production of plant disease control hydration agent composition The bacterial cell suspension of W-14-1 strain was freeze-dried. The viable count of the dried product is 1.0 × 10 ¹¹ cfu / g. 10 parts by weight of the dried bacteria body, 9 parts by weight of polyoxyethylene allylphenyl ether ammonium sulfate, and 81 parts by weight of calcium sulfate dihydrate were uniformly mixed to obtain a hydration agent composition (hereinafter, referred to as "W- 14-1 Hydrating Agent "). The hydrating agent composition is a medicament using P. nitrogen-producing strain W-14-1 as an active ingredient. [Experimental Example 3] Control effect test of lettuce putrefaction (bacterial disease) (field test) The hydrating agent of W-14-1 strain was diluted to 1000 times with water to prepare a treatment solution. The treatment liquid was sprayed on the lettuce transplanted in the field and treated. The spraying treatment was performed three times at intervals of one week. In addition, the pathogenic bacteria (Pseudomonas cichorii) of lettuce putrefaction was sprayed and inoculated with the aqueous suspension (A600 = 0.001) on the day of the first spraying treatment. One week after the third spray, the extent of the disease was investigated according to the following criteria, and the control value was calculated based on the following formulae 5 and 6. As a result, the control value was 65.0. Criterion 0: Non-onset Criterion 1: Part of the outer lobe can be seen Criterion 2: The outer lobe and part of the nodule can be seen Criterion 3: Most of the nodule can be seen to be diseased or more (Eq. 5) Incidence = ((( 1 × n1 + 2 × n2 ++ 3 × n3) / (3 × total number of surveyed plants)) × 100 n1 ～ n3 is the number of plants corresponding to each of the benchmarks 1 ～ 3 (Equation 6) Control value = (1-(of the treatment area) Incidence rate / incidence rate in untreated areas)) × 100 [Test Example 4] Test for controlling citrus canker disease (bacterial disease) (field test) The hydrating agent of W-14-1 strain was prepared by diluting it with water to 1000 times. Treatment solution. The treatment solution was sprayed on the whole navel orange tree and treated. The spraying treatment was performed twice in the initial stage of leaf growth and every two weeks thereafter, for a total of three times. Nine days after the final spraying, the incidence of citrus canker disease (pathogen Bacteroides xanthomonas: Xanthomonas campestris pv. Citri) on navel orange leaves was investigated according to the following criteria, and calculated based on the following formulas 7 and 8 Incidence and control value. As a result, the control value was 62.5. Criterion 0: No lesions Criterion 1: Number of lesions 1 to 3 Criterion 3: Number of lesions 4 to 10 Criterion 5: Number of lesions 11 to 20 Criterion 7: Number of lesions 21 or more ( (Equation 7) Incidence rate = ((1 × n1 + 3 × n3 + 5 × n5 + 7 × n7) / (7 × total number of survey leaves)) × 100 n1, n3, n5, and n7 are each of benchmark 1, 3, 5, and 7 Corresponding number of leaves (Eq. 8) Control value = (1-(incidence rate in treated area / incidence rate in untreated area)) × 100 [Test Example 5] Control effect test of lettuce spot bacterial disease (bacterial disease) (field test) ) Dilute the hydrating agent of W-14-1 strain to 1000 times with water to prepare a treatment solution. The spraying treatment was performed four times at intervals of one week. In addition, the pathogen of lettuce spot bacterial disease (Xanthomonas campestris pv. Vitians) was sprayed and inoculated with an aqueous suspension (A600 = 0.001) on the day of the first spray treatment. One week after the fourth spray, the degree of disease incidence was investigated according to the following criteria, and the incidence rate and the control value were calculated based on the following formulae 9 and 10. As a result, the control value was 87.9. Criterion 0: No onset criteria 1: Partial onset of outer leaves Criteria 2: Most onset of outer leaves Criterion 3: Onset of outer and inner leaves 4: Onset of nodule (Formula 9) Incidence rate = ((1 × n1 + 2 × n2 ＋ 3 × n3 ＋ 4 × n4) / (4 × total number of surveyed plants)) × 100 n1 ～ n4 are the number of plants corresponding to each of the benchmarks 1 ～ 4 (Eq. 10) Control value = (1— (incidence rate in treatment area) / Incidence rate in non-treated area)) × 100 [Test Example 6] Tomato gray mold (filamentous fungus disease) control effect test (field trial) The hydrating agent of W-14-1 strain was diluted to 1000 times with water and Prepare a treatment solution. The treatment liquid was sprayed on the tomatoes transplanted in the field and treated. The spraying treatment was performed four times at intervals of one week. In addition, the pathogen of tomato gray mold (Gray mold: Botrytis cinerea) was placed on the farmland on the day of the first spray treatment. Five surveys were conducted including the final survey six days after the fourth spray, and all young fruits were investigated for the onset of disease. In addition, the total number of results was counted at the time of the final survey, and the incidence rate and control value were calculated based on the following formulas 11 and 12. As a result, the control value was 89.9. (Equation 11) Incidence rate = (Number of incidence fruits / Total number of surveyed fruits) × 100 (Equation 12) Control value = (1-(Incitation rate in treated area / Incidence rate in untreated area)) × 100 [ Test Example 7] Pear black spot disease (filament fungus disease) control effect test (field test) The hydrating agent of the W-14-1 strain was diluted to 1000 times with water to prepare a treatment solution. The treatment liquid was sprayed on the whole pear tree transplanted in the field and treated. The spraying treatment was performed twice during the beginning of flowering and immediately after flowering. Two days after the second spraying, the presence or absence of pear black spot disease (pathogen Pear leaf spot fungus: Alternaria kikuchiana) on the leaves of the pear was investigated, and the diseased leaf rate and control value were calculated based on the following formulas 13 and 14. . As a result, the control value was 60.5. (Equation 13) diseased leaf rate = (number of diseased leaves / total number of leaves surveyed) × 100 (Equation 14) control value = (1-(the incidence of diseased leaves in the treatment area / the incidence of diseased leaves in the untreated area)) × 100 [ Test Example 8] Test for controlling citrus gray mold (filamentous fungus disease) (field test) The hydrating agent of strain W-14-1 was diluted to 1000 times with water to prepare a treatment solution. The treatment liquid was sprayed on the whole citrus tree transplanted in the farmland and treated. The spray treatment was performed twice during the initial leafing stage and the beginning of flowering. In addition, the pathogenic bacterium (Botrytis cinerea) of citrus gray mold is a pumpkin fruit which will form its spores at 70% blooming period and is arranged in the farmland. At the leaf-dropping stage, the presence or absence of disease on citrus flowers was investigated, and the incidence of flower incidence and control value were calculated based on the following formulae 15 and 16. As a result, the control value was 70.3. (Eq. 15) The incidence of flowering rate = (the number of flowers in the disease / the total number of flowers in the survey) x 100 (Equation 16) the control value = (1-(the incidence of flowering in the treatment zone / the incidence of flowering in the untreated zone)) x 100 [ Test example 9] Test for controlling citrus scab (filamentous fungus disease) control effect (field test) The hydrating agent of strain W-14-1 was diluted to 1000 times with water to prepare a treatment solution. The treatment liquid was sprayed on the whole citrus tree transplanted in the farmland and treated. The spraying treatment was performed three times at the initial leafing stage, the beginning of flowering stage, and the falling leaf stage. Thirteen days after the third spray, the incidence of citrus scab on the citrus leaves (pathogenic bacteria Elsinoe fawcettii) was investigated according to the following criteria, and the incidence was calculated based on the following formulae 17 and 18 And prevention value. As a result, the control value was 68.8. Criterion 0: No lesions Criterion 1: Number of lesions 1 to 5 Criterion 3: Number of lesions 6 to 20 Criterion 5: Number of lesions 21 to 50 Criterion 7: Number of lesions 51 or more ( (Equation 17) Incidence rate = ((1 × n1 + 3 × n3 + 5 × n5 + 7 × n7) / (7 × total number of survey leaves)) × 100 n1 ～ n7 are the number of leaves corresponding to each of the benchmarks 1-7 (Equation 18) Control value = (1-(incidence rate in treated area / incidence rate in non-treated area)) × 100 [Test Example 10] Control effect test of potato early blight (filamentous fungus disease) (field test) Will be W-14-1 The hydrating agent of the plant was diluted to 1000 times with water to prepare a treatment solution. The treatment liquid was sprayed on the potatoes transplanted in the field and treated. Performed twice at 10-day intervals. Four days after the second spraying, the extent of the incidence of early potato blight on potato leaves (pathogenic bacteria: Alternaria solani) was investigated according to the following criteria, and the incidence rate and Prevention value. As a result, the control value was 52.2. Criterion 0: No diseased spot on the leaf. Criterion 1: The diseased area does not reach 5% of the leaf area. Criterion 2: The diseased area does not reach 5 to 25% of the leaf area. Criterion 3: The diseased area does not reach 25 to 50 of the leaf area. % Baseline 4: The lesion area is 50% or more of the leaf area (Equation 19) Incidence rate = ((1 × n1 + 2 × n2 + 3 × n3 + 4 × n4) / (4 × total number of surveyed plants)) × 100 n1 ～ n4 The number of plants corresponding to each of the standards 1 to 4 (Equation 20) Control value = (1-(incidence rate in the treatment area / incidence rate in the non-treatment area)) × 100 As described above, it is determined that the microorganism is responsible for the bacterial disease. At least lettuce soft rot, cabbage soft rot, lettuce putrefaction, citrus canker, lettuce spot bacterial disease, effective for at least tomato gray mold, pear black spot, citrus gray mold, citrus scab And early blight of potato is effective. It was found that such a method has a high control effect on both bacterial diseases and filamentous fungus diseases. In addition, it was found that if such a bacillary disease and filamentous fungus disease control effect is obtained, the potato can have its effects on soft rot (bacteria) and early blight (filamentous bacteria) by the microorganism alone. This microorganism alone can have an effect on ulcer disease (bacteria) and gray mold (filamentous fungus), or ulcer disease (bacterial) and scab (filamentous fungus) at the same time. Bacteria) and gray mold (filament fungus), putrefaction (bacterial) and gray mold (filament), or spot bacterial disease (bacteria) and gray mold (filament).

FIG. 1 is a diagram showing a phylogenetic tree of the Pseudomonas nitrogen-producing W-14-1 strain (Pseudomonas nitrogen-producing NITE BP-02371) of the present invention, which was prepared based on the nucleotide sequence of the 16S rDNA gene.

[Proposal of the use of biological materials 1] [Host country] JP Japan [Host organization] Independent administrative corporation product evaluation technology base agency licensed microbiological trust center [Host date] 2016/10/12 [Host number] NITE BP-02371

Claims (4)

A nitrogen-producing Pseudomonas W-14-1 strain NITE BP-02371 or a variant thereof. A method for controlling plant diseases, which is characterized in that plants and / or cultivated soils of the plants are treated by using a nitrogen-producing Pseudomonas W-14-1 strain NITE BP-02371 or a mutant strain thereof. A plant disease control agent, which contains Pseudomonas nitrogen-producing W-14-1 strain NITE BP-02371 or a mutant strain thereof, and a surfactant. For example, a plant disease control agent according to claim 3, wherein the formulation ratio of the surfactant is 0.01 to 30 parts by mass relative to 1 part by mass of Pseudomonas nitrogen-producing W-14-1 strain NITE BP-02371 or a variant thereof. .