TW201628635A - An herbal composition for improving diabetic retinopathy and a use of the herbal composition thereof - Google Patents

Abstract

An herbal composition for improving diabetic retinopathy is disclosed. The herbal composition includes 40 wt% of an ethanol extract of Astragalus membranaceus, 8 wt% of an ethanol extract of Angelica sinensis and 52 wt% of a water extract of Dendrobium officinale. A use of the herbal medication is also disclosed.

Description

Herbal composition for improving diabetic retinopathy and use thereof

The present invention relates to a Chinese herbal medicine composition, in particular to a herbal composition for improving diabetic retinopathy, and to the use of the Chinese herbal medicine composition for preparing a medicament for improving diabetic retinopathy.

Diabetic retinopathy (diabetic retinopathy) is a complication of diabetes, which is caused by hyperglycemia-induced vascular wall insufficiency, thus affecting the function of the blood-retinal barrier and enhancing the blood vessels of the affected part. Symptoms caused by changes in retinal microcirculation, such as permeability, are the leading cause of blindness in diabetic patients.

The methods used to improve diabetic retinopathy include laser surgery, vitrectomy, and intraocular injection of corticosteroids. All three are invasive medical behaviors, and still cannot achieve the effect of radical cure. You need to monitor your blood sugar at any time and keep your blood sugar in the normal range (euglycemia), and the administration of corticosteroids is more likely to cause side effects. Accordingly, there is still a need for improved methods for treating diabetic retinopathy.

Dendrobium officinale can be used to lower blood sugar, thereby controlling diabetes-related diseases. It can also be used as an active ingredient in the treatment of retinal diseases, but its efficacy in improving diabetic retinopathy remains unclear.

The present invention provides a Chinese herbal medicine composition as an active ingredient for improving diabetic retinopathy.

The invention further provides a use of a Chinese herbal medicine composition as an active ingredient for improving diabetic retinopathy to improve the side effects caused by the conventional method for treating diabetic retinopathy.

A herbal composition for improving diabetic retinopathy comprises: 40% by weight of a sassafras extract, 8% of an angelica extract and 52% of an aqueous extract of Dendrobium.

The herbal composition for improving diabetic retinopathy according to the present invention, wherein the extract of the sassafras wine is extracted from a root sample of scutellaria, and is obtained by extracting an aqueous solution of ethanol having a volume percentage of 95%.

The herbal composition for improving diabetic retinopathy of the present invention, wherein the Angelica sinensis extract is extracted from a root sample of Angelica sinensis, and is obtained by extracting an aqueous solution of ethanol having a volume percentage of 95%.

The herbal composition for improving diabetic retinopathy according to the present invention, wherein the aqueous extract of Dendrobium is extracted from a stem sample of Dendrobium, and the Dendrobium aqueous extract is obtained by extracting a sample of Dendrobium stem from pure water.

The herbal composition for improving diabetic retinopathy according to the present invention, wherein the aqueous extract of Dendrobium is obtained by a method comprising the steps of: extracting the sample of the sarcophagus stem with pure water at 50 to 80 ° C, Obtaining a Dendrobium extract; and extracting and extracting the Dendrobium aqueous extract with an aqueous solution of 95% by volume of ethanol at 50-80 ° C to obtain a precipitate, the precipitate being the Dendrobium water Extract.

A use of a Chinese herbal medicine composition for preparing a medicament for improving diabetic retinopathy, the Chinese herbal medicine composition being administered to a desired organism to improve the desired lifting object The phenomenon of diabetic retinopathy, wherein the Chinese herbal medicine composition is a herbal composition as described above.

The use of the herbal composition of the present invention, wherein the Chinese herbal medicine composition is administered to the individual in need at a dose of 100 mg per kg of the desired individual per day, and is administered continuously for 15 to 60 days.

The herbal composition for improving diabetic retinopathy of the present invention is rich in ferulic acid and polysaccharide, can improve the phenomenon of diabetic retinopathy, and achieve the effect of preventing the patient's vision from continuously deteriorating.

The use of the herbal composition of the present invention is a derivative of a herbal composition rich in ferulic acid and a polysaccharide as an active ingredient for improving diabetic retinopathy and preventing side effects caused by conventional drugs, and is an effect of the present invention.

Fig. 1 shows changes in intraocular pressure on the 1st, 15th, 30th, 45th and 60th day of the rats in the C0~C4 group.

Fig. 2a: Photograph of the fundus of the group C0 rats on the first day.

Figure 2b: Results of fundus photography on day 15 of group C0 rats.

Figure 2c: Photograph of the fundus of the group C0 rats on the 30th day.

Figure 2d: Results of fundus photography on day 45 of group C0 rats.

Figure 2e: Results of fundus photography on day 60 of group C0 rats.

Fig. 3a: Results of fundus photography on day 1 of group C1 rats.

Fig. 3b: Results of fundus photography on day 15 of group C1 rats.

Figure 3c: Results of fundus photography on day 30 of group C1 rats.

Fig. 3d: Results of fundus photography on day 45 of group C1 rats.

Fig. 3e: Results of fundus photography on day 60 of group C1 rats.

Figure 4a: Results of fundus photography on day 1 of group C2 rats.

Figure 4b: Results of fundus photography on day 15 of group C2 rats.

Figure 4c: Results of fundus photography on day 30 of group C2 rats.

Fig. 4d: Photograph of the fundus of the group C2 rats on the 45th day.

Figure 4e: Results of fundus photography on day 60 of group C2 rats.

Fig. 5a: Results of fundus photography on day 1 of group C3 rats.

Figure 5b: Results of fundus photography on day 15 of group C3 rats.

Figure 5c: Results of fundus photography on day 30 of group C3 rats.

Fig. 5d: Results of fundus photography on day 45 of group C3 rats.

Figure 5e: Results of fundus photography on day 60 of group C3 rats.

Fig. 6a: Results of fundus photography on day 1 of group C4 rats.

Figure 6b: Results of fundus photography on day 15 of group C4 rats.

Figure 6c: Results of fundus photography on day 30 of group C4 rats.

Fig. 6d: Results of fundus photography on day 45 of group C4 rats.

Figure 6e: Results of fundus photography on day 60 of group C4 rats.

The above and other objects, features and advantages of the present invention will become more <RTIgt; The composition comprises: 40% by weight of a sassafras extract, 8% of an angelica extract and 52% of an aqueous extract of Dendrobium, and the fermented by the Chinese herbal composition Active ingredients such as acid and polysaccharide can improve diabetic retinopathy in patients.

The extract of the sassafras wine, the extract of the angelica wine and the extraction method of the aqueous extract of the sarcophagus are adjusted according to the needs of those having ordinary knowledge in the technical field of the invention, and are not limited thereto. In detail, the extract of the sassafras wine is extracted from a root sample of a scutellaria, for example, extracted by an aqueous solution of 95% by volume of an aqueous solution of ethanol; The root sample can also be obtained by extracting from a 95% by volume aqueous solution of ethanol. In the present example, 1 kg of the xanthium root sample and 200 g of the angelica root sample were mixed, and then extracted with 4 L of an aqueous ethanol solution (95% by volume).

Further, the Dendrobium water extract is extracted from a stem sample of a Dendrobium, and is obtained by extracting pure water, for example, the stem sample of the Dendrobium can be extracted with pure water at 50-80 ° C to obtain a Dendrobium extract. The aqueous extract of Dendrobium candidum and the aqueous solution of ethanol (95% by volume) were subjected to partition extraction at 50-80 ° C to obtain a precipitate, which is the aqueous extract of Dendrobium. In the present embodiment, a 1 kg sample of Dendrobium candidum was mixed with 4 L of pure water, and the extract was heated for 2 hours, and the obtained Dendrobium extract was concentrated under reduced pressure to a volume of 1 L, and 3 L of an aqueous ethanol solution (volume percent concentration) was continuously added. It is 95%, preferably placed at 4 ° C to cool down. After one night of precipitation, it is centrifuged at 12,000 rpm for 20 minutes. After removing the supernatant, the aqueous extract of Dendrobium can be obtained.

The herbal composition of the present invention can effectively repair the damage of retinal cells caused by hyperglycemia, and thus can be used as an active ingredient for improving diabetic retinopathy, for example, the Chinese herbal medicine composition can be applied to prepare a drug for improving diabetic retinopathy. Or a health food product, the Chinese herbal medicine composition is combined with a pharmaceutically acceptable carrier or excipient to form a pharmaceutical composition, wherein the Chinese herbal medicine composition can be prepared into any convenient form such as a tablet, a capsule, or the like. A powder, a granule or a liquid, or the like, or a combination of the Chinese herbal composition with other foods or beverages, for oral administration to the organism in a form suitable for consumption.

Further, the Chinese herbal medicine composition can be administered to a desired individual, for example, by administering 100 mg of the Chinese herbal medicine composition per kg of the desired individual body weight per day, and continuously administering the desired individual for 15 to 60 days to make the Awei Active ingredients such as acids and polysaccharides can act in the desired individual, and can improve the diabetic retinopathy of the desired individual.

In order to confirm that the Chinese herbal medicine composition of the present invention does have an activity of improving the phenomenon of diabetic retinopathy, the following tests were carried out:

(A) Preparation of Chinese herbal medicine composition

In this test, 1 kg of Scutellaria root samples and 200 g of Angelica root samples were mixed, and extracted with 4 L of ethanol solution (95% by volume) for 800 minutes, repeated three times, vacuum filtered, concentrated under reduced pressure. After lyophilization, a total of 98.52 g of the mixed wine extract (i.e., the mixture of the sassafras extract and the extract of the angelica extract) was obtained.

In addition, 4 L of pure water was mixed with 1 kg of the stem sample of Dendrobium, heated and extracted for 2 hours, and the obtained Dendrobium extract was concentrated under reduced pressure to a volume of 1 kg, and a 3 L aqueous solution of ethanol was added (95% by volume, priority After cooling at 4 ° C, the precipitate was centrifuged at 12,000 rpm for 20 minutes, and after removing the supernatant and drying, 74.58 g of the aqueous extract of Dendrobium was obtained.

The mixed extract and the aqueous extract of Dendrobium sinensis are continuously mixed to obtain the herbal composition of the test, wherein the Chinese herbal medicine composition comprises 40% by weight of the extract of sassafras, 8% of the extract of Angelica sinensis And 52% of the water extract of Dendrobium.

(B) Detection of active ingredient content of Chinese herbal medicine composition

The ferulic acid content of the Chinese herbal medicine composition was determined by dissolving 1.608 g of the Chinese herbal medicine composition in methanol, and then filtering and concentrating with a 0.45 μm filter, and then adjusting the total volume to 10 mL with methanol as a substitute sample. Continued selection of Thermo ODS Hypersil (5μm) 250mm*4.6mm column, mixing acetonitrile (Acetonitrile, ACN for short) and 0.05% phosphoric acid solution as mobile phase at a flow rate of 1mL/min. The absorbance at a wavelength of 320 nm, and the percentage of ferulic acid in the total area, and the calibration curve drawn from the known concentration of ferulic acid solution, the result shows that the ferulic acid content of the Chinese herbal composition is 42.255. ±5.655 μg/g.

Further, the polysaccharide content of the Chinese herbal medicine composition is determined by a phenol-sulfuric acid method, and after diluting 200 mg of the Chinese herbal medicine composition in 100 mL of hot water, the volume is 0.3 mL, 0.4 mL, and 0.5 mL, respectively. 0.6mL, 0.7mL, 0.8mL of the dilution, add an equal volume of 5% phenol solution and 5 times the volume of concentrated sulfuric acid solution, mix and oscillate After standing for 30 minutes, the absorbance at a wavelength of 490 nm was detected, and the polysaccharide content of the Chinese herbal medicine composition was found to be 14.667 μg/g (see 〝 per gram of Chinese herbal medicine). The glucose content of the composition is used as a basis for comparison).

(C) Effects on diabetic retinopathy in diabetic rats

In this experiment, Wistar male rats purchased from the National Experimental Animal Center of the National Experimental Research Institute of the National Research Institute were selected. These rats were all 6 to 8 weeks old and weighed between 150 and 200 grams. The rats were housed in the animal center of Daren University of Science and Technology, and maintained an animal room with a room temperature of 24 ± 2 ° C and a relative humidity of 65 ± 5%. The light time and dark time were each 12 hours. The rats were free to eat. And drinking water.

Please refer to the first table, the rats are divided into the C0~C4 group, and the rats in the C1~C4 group are subjected to intraperitoneal injection (intraperitioneal injection) for 72 hours. Streptozotocin (STZ for short) was administered to the rats (administered at a dose of 60 mg/kg) to induce the formation of diabetic rats. After 72 hours, the blood glucose levels of the rats were measured and the blood glucose level was high. Rats at 300 mg/L and with more than three symptoms (eat more, drink more, more urine) can be considered to be successfully induced into type 1 diabetic rats.

The Chinese herbal medicine composition as shown in Table 1 was orally administered to each group of rats, fed once a day for 60 days, and the diabetic retinopathy was evaluated separately.

On days 1, 15, 30, 45 and 60, rats in each group were anesthetized with ether, respectively. The intraocular pressure was measured, and as shown in Fig. 1, the eye pressure of the C1 group rats was maintained at about 20 mmHg from the 15th day, and the continuous feeding of the Chinese herbal medicine composition (i.e., the C4 group) was significantly reduced. The intraocular pressure value of the rat.

On the 1st, 15th, 30th, 45th and 60th day, after atropine (0.25%) eye drops were added, each group of diabetic rats was anesthetized with ether, and after 5 minutes, the ophthalmoscope was used ( MiiS Horus Scope DOC100) The fundus photography of each group of diabetic rats was performed as shown in Fig. 2a~2e (C0 group rats), 3a~3e pictures (C1 group rats), and 4a~4e pictures ( Group C2 rats), pictures 5a-5e (group C3 rats), and pictures 6a-6e (group C4 rats), shown.

Please refer to the figures 2a~2e. The retina of the group C0 does not show signs of diabetic retinopathy. Continue to refer to the 3a~3e chart. The rats in the C1 group start to develop the diabetic retina after the 30th day. Symptoms of lesions, such as intraretinal microvascular abnormalities (IRMA) and retinal hemorrhage, please refer to Figures 4a~4e and 5a~5e, in groups C2 and C3. Although there were signs of diabetic retinopathy after 30 days, the state was slightly more moderate than that of the C1 group, and the retinal state of the C4 group shown in Fig. 6a~6e was not the same as that of the group C0. The difference is that the Chinese herbal medicine composition of the present invention can indeed improve the state of diabetic retinopathy.

In summary, the herbal composition for improving diabetic retinopathy of the present invention is rich in ferulic acid and polysaccharide, which can improve the phenomenon of diabetic retinopathy and prevent the patient from continuously improving the vision.

Furthermore, the use of the herbal composition of the present invention is a derivative of a herbal composition rich in ferulic acid and a polysaccharide as an active ingredient for improving diabetic retinopathy and preventing side effects caused by conventional drugs, and is an effect of the present invention. .

Although the present invention has been disclosed using the above preferred embodiments, it is not intended to be limiting The present invention is not limited to the spirit and scope of the present invention, and various modifications and changes to the above embodiments are still within the technical scope of the present invention. Therefore, the scope of protection of the present invention is attached. The scope defined in the scope of application for patent application shall prevail.

Claims (7)

A herbal composition for improving diabetic retinopathy comprises: 40% by weight of a sassafras extract, 8% of an angelica extract and 52% of an aqueous extract of Dendrobium. The herbal composition for improving diabetic retinopathy according to claim 1, wherein the extract of the sassafras is extracted from a root sample of scutellaria and is 95% by volume. It is obtained by extraction with an aqueous solution of ethanol. The herbal composition for improving diabetic retinopathy according to claim 1, wherein the angelica extract is extracted from a root sample of Angelica sinensis and is one of 95% by volume ethanol. Extracted from an aqueous solution. The herbal composition for improving diabetic retinopathy according to claim 1, wherein the aqueous extract of Dendrobium is extracted from a stem sample of Dendrobium, and the aqueous extract of Dendrobium is extracted from pure water. Stem samples were obtained. The herbal composition for improving diabetic retinopathy as described in claim 4, wherein the aqueous extract of Dendrobium is obtained by a method comprising the steps of: extracting at 50-80 ° C with pure water The sarcophagus stem sample is obtained to obtain a sarcophagus extract; and the aqueous extract of the sarcophagus is distributed and extracted with an aqueous solution of 95% by volume of ethanol at 50-80 ° C to obtain a precipitate. The precipitate is the aqueous extract of Dendrobium. A use of a Chinese herbal medicine composition for preparing a medicament for improving diabetic retinopathy, wherein the Chinese herbal medicine composition is administered to a desired organism to improve the diabetic retinopathy of the desired ascending object, wherein the Chinese herbal medicine composition The herbal composition according to any one of the items 1 to 5 of the patent application. The use of the herbal composition according to claim 6, wherein the Chinese herbal composition is administered at a dose of 100 mg per kg of the desired individual per day. Body, and continuous administration for 15 to 60 days.