TW201429501A - A composition for promoting hair growth - Google Patents

Abstract

The present invention provides a composition for promoting hair growth in a subject, comprising transferrin in an amount effective for at least one of promoting dermal papilla proliferation and activating hair shaft growth.

Description

Composition for promoting hair growth

The present invention relates to a composition for promoting hair growth, and more particularly to a use of a ferritin for the preparation of a composition for promoting hair growth.

Transferrin (Tf) is a glycoprotein containing 679 amino acids with a molecular weight of about 79 kDa. It is mainly synthesized by liver cells and is present in body fluids, including plasma, bile, amniotic fluid, and brain. Spinal fluid, lymph and milk. Ferritin can bind iron like lactoferrin, except that ferritin has the function of transporting iron ions. Its main function is to transport iron from absorption centers, such as duodenum and macrophages, to various tissues of the body. Red blood cell production and cell division play a key role.

In addition, ferritin is also associated with innate immunity. It has been found that the mucosa contains ferritin, which binds to iron and creates an environment of low iron ion concentration in the mucosa, preventing bacteria from obtaining iron. In order to inhibit the growth of bacteria, the concentration of ferritin is also reduced when inflamed.

The concentration of ferritin in plasma is stable from birth, about 2-3 grams per liter, and has a half-life of up to 8 days in the body. There is a great relationship between the concentration of ferritin and growth. Concentrations below 0.1 gram per liter increase the chance of infection, growth retardation and anemia. According to the study, ferritin is involved in cell growth, differentiation and cell protection. Some important results show that ferritin synergizes with other growth factors to regulate cell growth and differentiation. The combination of different growth factors may have a polarized response to the same cells that stimulate or inhibit cell growth.

A novel ferritin-rich peptide is disclosed in a published U.S. Patent (US Pat. Protein to promote hair growth.

There are many factors that cause hair loss, such as immune disorders, exposure to toxic chemicals, stress, burns, or skin diseases. However, the most common cause is aging or genetics. Hair loss. Typical methods for promoting hair growth focus on controlling cell death or promoting regeneration. Although many researches have been carried out on hair growth agents, the effects of traditional hair growth agents on hair growth, that is, avoiding hair loss and promoting hair growth, are insufficient. .

Currently, dermatologist-approved methods for the treatment of hair loss or bald include oral Propecia (Proscar) and topical Rogaine, which is the only drug currently approved by DA in the United States for the treatment of hair loss. Applicable to Rogaine. Rope is the only oral drug approved for the treatment of male head and forehead hair loss, but has no effect on female users and may have side effects that impair male sexual function. Founded also known as Minoxidil, it stimulates the growth of the baldness of the male head and promotes hair growth in the forehead area of female users. However, when the patient has an irritated or abnormal scalp, the scalp may increase the absorption of senoxide, resulting in side effects such as decreased blood pressure, increased heart rate, and increased body weight (corporeal fluid retention). In addition, the main ingredients of alcohol in the construction will stimulate eye discomfort.

Therefore, there is still a need for a composition using a hair growth promoter to promote hair growth without causing the above-mentioned side effects.

The present invention provides a composition for promoting growth of a body hair, characterized in that the composition comprises an effective amount of ferritin.

In one embodiment of the invention, the amount of ferritin is from 1 microgram/ml to 200 mg/ml.

Preferably, the composition can be applied to or within the skin area of the individual by one of application, spraying, evaporating or spraying.

Preferably, the composition is applied by soaking or bathing the individual in the composition.

Preferably, the application of the composition is applied topically to the skin of the individual.

Preferably, the composition can be formulated as an ointment, shampoo, conditioner, lotion, hair tonic, hair gel or mousse.

Preferably, the composition may further comprise a pharmaceutically acceptable carrier.

The invention also provides a use of a ferritin for the preparation of a composition for promoting the growth of a body hair.

These and other aspects of the present invention will be apparent from the following description of the preferred embodiments of the invention and Modification.

Figure 1 shows that ferritin can significantly promote the growth of hair follicles. (A) is a microscopic image of the hair follicle tissue of Wistar rats, which shows that hair follicle tissue treated with a concentration of 100 ug/ml can significantly promote the growth of hair follicles; (B) is the dry length of the hair of Figure (A) As a result of the measurement, the dry growth length on the 5th day after the treatment was increased by 45% compared with the PBS control group. ^* p<0.05.

Figure 2 shows the results of MTT measurement of dermal papilla cell proliferation, showing that ferritin can significantly increase the proliferation of dermal papilla tissue, and the effect is more significant with the increase of ferritin concentration. ^* p<0.05, ^** p<0.001.

All technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art, unless otherwise defined. In case of conflict, this document, including its definition, is the main one.

As used herein, the articles "a" or "the" are used to mean that the grammatically accepted words are one or more (ie, at least one). For example, "an element" means one element or more than one element.

As used herein, "hair follicle" means a hair structure located under the skin that is a saclike structure that extends from the epidermis down into the dermis, including the epithelial portion and the dermal portion. The epithelial portion contains an inner root sheath and an outer root sheath, and the dermis portion contains a dermal papilla and a dermal sheath. Hair growth progresses through the anagen, catagen, telogen, and exogen, and this cycle is repeated throughout life. During the growth period, the hair follicles are active in the growth phase, and the cells at the root of the hair divide rapidly and increase to the hair shaft; the degeneration period refers to the transient transition phase at the end of the growth phase, indicating the end of the growth phase; the termination phase is the stationary phase of the hair, At this point the hair follicle is completely terminated and forms a golf club shape, meaning that the hair root is completely surrounded by a bulbous enlargement consisting of keratinocytes.

The present invention provides a composition for promoting the growth of a body hair, including at least One of the effective amount of ferritin in the activation of hair follicles of the individual and the proliferation of dermal papilla tissue. As used herein, "individual" means human or animal having hair, particularly warm-blooded animals and birds having hair follicle tissue.

By "effective amount" is meant an amount that provides an effect that promotes hair growth to an individual, and those skilled in the art can readily determine the effective amount, as well as the dosage and frequency of administration, in accordance with the teachings of the present invention, using their knowledge and standard methods of routine experimentation. .

As used herein, "ferroprotein" includes bovine ferritin, human ferritin or a ferritin extracted from other species, a synthetic ferric protein, and a derivative of the above-described ferritin.

The present invention has found that ferritin can promote the growth of hair follicles, and can also significantly promote the proliferation of dermal papilla cells, which are the main tissues for hair growth of dermal papilla cells. Therefore, the present invention provides a composition effective for promoting hair growth.

The composition comprising ferritin can be applied to the skin of a body by any means known in the art including, but not limited to, topical application, spraying, bubbling or spraying a composition comprising ferritin to promote hair growth. On or in the skin of an individual. In addition, the individual may also be soaked or bathed in a composition comprising ferritin. According to the present invention, the ferritin content of the composition may range from about 1 microgram/ml to 200 mg/ml.

Compositions containing ferritin can be made by mixing ferritin with other active or inactive ingredients in a variety of forms for ease of use. Other ingredients may include, without limitation, an adjuvant such as an adjuvant, a vehicle or excipient, a perfume, a colorant, a stabilizer, or other inactive ingredients, or Whether or not it contains any combination of other inactive ingredients. Preferred inactive ingredients include, for example, dimethyl hydrazine (DMSO), glycerol, collagen, and hyaluronan (HA). According to a non-limiting embodiment, the composition can be formulated in the form of an ointment, shampoo, conditioner, lotion, hair tonic, hair gel or mousse. The compositions of the present invention may comprise different added active ingredients and supplemental ingredients commonly used in topical formulations, such as growth factors, hormones, and cell proliferating factors known to those skilled in the art, which do not affect ferritin levels. Promotes hair growth.

The invention also provides a novel use of ferritin for promoting hair growth in an individual.

The invention is further illustrated by the following examples, which are provided for purposes of illustration and not limitation. It will be apparent to those skilled in the art that <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt;

Example Example 1 Ferritin promotes the growth of hair follicles

In order to study the effect of ferritin on hair growth, a six-week-old Wistar rat (purchased from the Animal Center of National Taiwan University of Medicine) was intraperitoneally injected with 7% chloral hydrate to cut off the skin of the cheeks. The cells were washed with 1X PBS, placed in DMEM medium containing 2.5 ug/ml fungizone and stored at 4 ° C, and the intact hair follicles of the whiskers were separated by ophthalmic surgical scissors and forceps. The isolated murine hair follicles were each cultured in PBS (control group) or PBS containing 100 ug/ml of ferritin. After 5 days of culture, the growth length of the hair follicles was measured by microscopic observation. As shown in Fig. 1A and Fig. 1B, the treatment of hair follicles with ferritin was remarkable compared with the control group without ferricin. It promotes the growth of hair follicles by 45%, indicating that the use of an effective amount of ferritin can promote hair growth.

Example 2 Ferritin promotes proliferation of dermal papilla cells

To further test the effect of ferritin on dermal papilla cells, the isolated hair follicle roots were shredded and labeled with type 1 collagenase (2 mg/ml type I collagenase in 1% FBS/DMEM solution). The reaction was carried out for 2 hours at 37 ° C in a 5% carbon dioxide incubator until the outer root sheath was loosened. The reaction was stopped in DMEM medium containing 10% FBS, centrifuged and the supernatant was aspirated to remove collagenase, and re-separated with DMEM containing 20% FBS. The dermal papilla cells in suspension were cultured, and when the cells were attached and spread outward, the fresh medium was replaced every 3 days, and the test was carried out 7 days after the culture.

Before testing the effect of ferritin, the dermal papilla cells were seeded in a 24-well plate at a cell concentration of 2×10 ⁴ , and after 16 hours of culture, the culture solution in the well was discarded, and 500 μl of DMEM medium was added after washing with PBS (excluding FBS) continued to culture for 16 hours. After 16 hours, the wells were aspirated and washed with PBS, followed by addition of DMEM containing different concentrations of ferritin (without FBS) for 16 hours, and the control group was replaced with PBS for ferritin. After treatment with different concentrations of ferritin for 16 hours, the well culture solution was aspirated and washed with PBS, then 200 μl of MTT solution was added to each well for 2 hours. The solution in the well was aspirated and 150 μl of DMSO was added to dissolve and crystallize at 540. The absorbance was measured at nm to analyze cell proliferation.

As shown in Fig. 2, cell proliferation was observed in the dermal papilla cells with 1, 10, 50, 100, 200 ug/ml ferritin, respectively, compared with the PBS control group, with 50 ug/ml applied. The number of proliferating cells of ferritin-producing dermal papilla cells was 1.7 times that of the PBS-controlled group, and when the concentration of ferritin was increased to 100 ug/ml, the number of cell proliferation was 2.3 times that of the PBS-controlled group, and when the concentration of ferritin reached 200 ug. At /ml, the number of dermal papilla cells proliferates up to 3.2 times. Therefore, the results show that administration of an effective amount of ferritin can significantly increase the proliferation of the dermal papilla tissue at the bottom of the hair follicle, making the hair thicker and accelerating dry growth.

The present invention therefore provides a composition effective to promote hair growth in an individual without the limitations of gender or age. After administration of the composition containing the effective amount of ferritin to the isolated hair follicle tissue and the dermal papilla cells, a significant increase in hair follicle dryness and significant proliferation of the dermal papilla tissue can be observed, so that the composition can be applied to the individual effectively Promote its hair growth. Moreover, those skilled in the art will recognize from the teachings of the present invention that ferritin and other derivatives may also be used in the compositions of the present invention to promote hair growth in individuals who develop hair loss due to disease or natural factors.

Although the results of the above examples show a new use of ferritin to promote hair growth in Wistar breed rats, the scope of the present invention should not be limited thereto. Those skilled in the art will be able to understand the present invention and apply it to any warm-blooded animal having hair follicles, including humans, cows, pigs, horses, sheep, dogs, mice, rats, rabbits, and birds, such as chickens. , ducks, geese and turkeys.

The present invention will be applied to its broadest scope without further elaboration, based on the description herein. Therefore, it is to be understood that the claims and claims are not intended to limit the scope of the invention.

Claims (9)

A composition for promoting hair growth of a body comprising an effective amount of a transferrin. The composition of claim 1, wherein the ferritin is used in an amount of from 1 μg/ml to 200 mg/ml. The composition of claim 1, wherein the composition is applied to the skin area or the skin area of the individual by at least one of application, spraying, steaming or spraying. The composition of claim 1, wherein the composition is applied by soaking or bathing the individual in the composition. The composition of claim 1, wherein the application of the composition is applied to the skin of the individual by topical application. The composition of claim 1, wherein the composition is formed into a type selected from the group consisting of ointment, shampoo, conditioner, lotion, hair tonic, hair gel or mousse (mousse) ) ethnic groups. The composition of claim 1, wherein the composition further comprises a pharmaceutically acceptable carrier. The composition of claim 1, wherein the system is a warm-blooded animal having hair follicles. A use of a ferritin for the preparation of a composition for promoting the growth of a body hair.