201102640 九、發明說明： 【發明所屬之技術領域】 本發明係有關於一種電化學生化檢測試片的非破壞性檢測方法，尤指一 種在生產線上於生產進行中，利用檢測導線與橫跨工作電極與參考電極之 間的電阻，以找出不良電化學生化檢測試片，並據此將每片檢測試片訂定 編碼（CODE)分類的方法。 【先前技術】 一般血糖檢測試片都是將銀膠及碳膠利用網板印刷方法將導線網印在 PET或PVC塑膠片上，然後再將它烘烤讓銀膠或碳膠乾燥固化。然而，在 網印的過程，由於銀膠或碳膠裡的溶劑不斷揮發，銀膠或碳膠的黏度 (Viscosity)會越來越黏稠，因而每一板印出來的銀膠或碳膠乾燥固化後的 特性都不同。因此，往往必須針對每一板上的眾多血糖檢測試片取出少量 血糖檢測試片作破壞性的測試，以找出該板其它血糖檢測試片的編碼 (CODE)分類。然後利用血糖檢測試片獨有的編碼（CODE)來修正血糖 檢測儀器的軟體參數，才能測得正確的血糖讀值。 目前習用的技術是利用測量每一條導線的電阻，如果導線測量出來的 讀值在能接受的範圍外，就被棄置不用。因而在實際生產時，可能會造成 百分之二十五到三十的試片廢料，增加生產成本。同時，因為每一板的銀 膠或碳膠導線特性都不同，每一板上的眾多血糖檢測試片必須取出少量血 糖檢測試片作破壞性的測試，以找出該板其它血糖檢測試片的編碼(CODE) 分類，除了要耗費很多人力之外，也會報廢很多試片，增加生產成本》因 201102640 此本發明就是在生產線上於生產進行中，利用檢測導線與橫跨工作電極與 參考電極之間的電阻，以找出不良電化學生化檢測試片，並據此將每片檢 測試片訂定編碼（CODE)分類，以降低生產成本。 【發明內容】 由於一般電化學生化檢測試片試利用對工作電極及參考電極間施加一 個電位差以引起電化學反應，並且測量其間的電化學反應以偵測代測物的 濃度。因此，橫跨工作電極與參考電極之間的電阻會決定該試片的電化學 特性。本發明是有關於一種只要利用施加固定電壓或固定電流來檢測每片 電化學生化檢測試片的導線與橫跨工作電極與參考電極之間的電阻，以找 出不良電化學生化檢測試片，並據此將每片檢測試片訂定編碼（CODE)分 類，以降低生產成本的方法。本創作之上述及其他目的與優點，可從下述 圖中.，獲得深入了解。 【實施方式】 如第一圖所示，一底板10上設有一導線20連接到工作電極25及一導線20 連接到參考電極30。在工作電極25及參考電極30上滴有一滴反應酵素70。 兩條導線的最左端分別放置兩根鍍金的探針40，每支探針40上端各連接一 條電線50。如第二圖所示，在實際量產時，每一片積材板15上會有很多片 上有導線20及反應酵素70的底板10。有一個電源供應器77透過記電器78 將固定的電源或電壓供應給其中一片底板10上的導線20，並經由導線20 將固定的電源或電壓施加在反應酵素70上，以測量橫跨工作電極25及參考 201102640 電極30間的電阻。第一片上有導線20及反應酵素70的底板10測量過電阻 之後，繼電器78會將電源供應器77固定的電源或電壓供應給另外一片底板 10上的導線20，並經由導線20將固定的電源或電壓施加在反應酵素70上， 以測量另外一片底板10上橫跨工作電極25及參考電極30間的電阻。繼電 器78會將電源供應器77固定的電源或電壓再供應給另外一片底板10以測 其電阻，直到每一片積材板15上所有底板10的電阻全測完。然後，再將所 有測得的結果匯整送到分析裝置80作資料判讀。如果有任何一片底板10 • 的電阻值與其他大部分的底板10的電阻值差異過大，則該片底板10則視為 不良品，予以另外分類。這些電阻值的分佈則再與資料庫的資料作比對， 可以得知每一片底板10的編碼（CODE)，再將這些資料顯示於資料顯示裝 置82。一般我們使用的固定電流為0.01mA~10mA，而固定的電壓為 0.01V〜2V，且每片測量時間為0.01〜1秒。 第一圖為以兩根探針40的兩線式方法測量底板10的電阻，而另一種可 行的方式是以四根探針40的四線式方法測量底板10的電阻，如第三圖所示。 • 由以上詳細說明，可使熟知本項技藝者明瞭本創作的確可達成前述目 的，實已符合專利法之規定，爰提出專利申請。 惟以上所述者，僅為本創作之較佳實施例而已，當不能以此限定本創作 實施之範圍；故，凡依本創作申請專利範圍及創作說明書内容所作之簡單 的等效變化與修飾，皆應仍屬本創作專利涵蓋之範圍内。 【圖式簡單說明】 第一圖，係本發明之一以兩根探針測量導線間的電阻的示意圖 201102640 第二圖，係本發明之數據分析處理示意圖 第三圖，係本發明另一以四根探針測量導線間的電阻的示意圖 【主要元件符號說明】 10·底板 20.導線 25.工作電極 30.參考電極 40.探針 50.導線 70.反應酵素 77. 電源供應器 78. 繼電器 79. 電阻計 80. 分析裝置 81. 資料庫比對 82. 顯示結果裝置201102640 IX. Description of the invention: [Technical field to which the invention pertains] The present invention relates to a non-destructive detection method for an electrochemical biochemical test strip, in particular, a production line in production, using a test lead and a cross-working The resistance between the electrode and the reference electrode to find a bad electrochemical biochemical test strip, and according to this method, each test strip is coded (CODE). [Prior Art] In general blood glucose test strips, silver glue and carbon glue are printed on a PET or PVC plastic sheet by screen printing, and then baked to make the silver glue or carbon glue dry and solidify. However, in the process of screen printing, since the solvent in the silver glue or the carbon glue is continuously volatilized, the viscosity of the silver glue or the carbon glue will become more and more viscous, and the silver glue or carbon glue printed on each board is dried and solidified. The characteristics are different. Therefore, it is often necessary to take a small amount of blood glucose test strips for each blood glucose test strip on each board for a destructive test to find the code (CODE) classification of other blood glucose test strips on the board. Then, using the unique code (CODE) of the blood glucose test strip to correct the software parameters of the blood glucose tester, the correct blood glucose read value can be measured. The current technique is to measure the resistance of each wire, and if the measured value of the wire is outside the acceptable range, it is discarded. Therefore, in actual production, it may cause 25 to 30 percent of test piece waste, increasing production costs. At the same time, because each board has different characteristics of silver glue or carbon glue wire, many blood glucose test pieces on each board must take a small amount of blood glucose test piece for destructive test to find other blood sugar test pieces of the board. The coding (CODE) classification, in addition to a lot of manpower, will also scrap a lot of test strips and increase production costs. Because of the 201102640, the invention is in production on the production line, using the test leads and the cross working electrodes and reference The resistance between the electrodes is used to find the defective electrochemical biochemical test strips, and each test strip is classified according to the code (CODE) to reduce the production cost. SUMMARY OF THE INVENTION The general electrochemical biochemical test strip test uses a potential difference between the working electrode and the reference electrode to cause an electrochemical reaction, and measures the electrochemical reaction therebetween to detect the concentration of the analyte. Therefore, the electrical resistance across the working electrode and the reference electrode determines the electrochemical characteristics of the test piece. The present invention relates to a method for detecting a poor electrochemical biochemical test piece by using a fixed voltage or a fixed current to detect a wire between each of the electrochemical biochemical test strips and a resistance across the working electrode and the reference electrode. According to this, each test piece is coded (CODE) to reduce the production cost. The above and other objects and advantages of the present invention can be obtained from the following figures. [Embodiment] As shown in the first figure, a substrate 10 is provided with a wire 20 connected to the working electrode 25 and a wire 20 connected to the reference electrode 30. A drop of the reaction enzyme 70 is dropped on the working electrode 25 and the reference electrode 30. Two gold-plated probes 40 are placed at the leftmost ends of the two wires, and one wire 50 is connected to the upper end of each of the probes 40. As shown in the second figure, in the actual mass production, each of the pieces 15 has a plurality of bottom plates 10 having wires 20 and reaction enzymes 70 thereon. A power supply 77 supplies a fixed power or voltage to the wires 20 on one of the substrates 10 via the power registers 78, and applies a fixed power or voltage to the reaction enzyme 70 via the wires 20 to measure across the working electrodes. 25 and reference 201102640 resistance between the electrodes 30. After the first piece of wire 20 and the bottom plate 10 of the reaction enzyme 70 are measured for electrical resistance, the relay 78 supplies a fixed power or voltage to the power supply 77 to the wire 20 on the other substrate 10, and is fixed via the wire 20. A power source or voltage is applied to the reaction enzyme 70 to measure the resistance across the other substrate 10 across the working electrode 25 and the reference electrode 30. The relay 78 supplies the fixed power or voltage of the power supply 77 to the other substrate 10 to measure its resistance until the resistance of all the substrates 10 on each of the stacked boards 15 is completely measured. Then, all the measured results are sent to the analyzing device 80 for data interpretation. If the resistance value of any one of the base plates 10 is excessively different from that of most of the other base plates 10, the base plate 10 is regarded as a defective product and is additionally classified. The distribution of these resistance values is then compared with the data of the database, and the code (CODE) of each of the substrates 10 is known, and then the data is displayed on the data display device 82. Generally, we use a fixed current of 0.01 mA to 10 mA, and a fixed voltage of 0.01 V to 2 V, and each measurement time is 0.01 to 1 second. The first figure shows the resistance of the bottom plate 10 by a two-wire method of two probes 40, and another feasible way is to measure the resistance of the bottom plate 10 by a four-wire method of four probes 40, as shown in the third figure. Show. • From the above detailed description, it will be clear to those skilled in the art that this creation can indeed achieve the above objectives, and it has already met the requirements of the Patent Law and has filed a patent application. However, the above is only the preferred embodiment of the present invention, and the scope of the creation of the present invention cannot be limited by this; therefore, the simple equivalent changes and modifications made by the scope of the patent application and the content of the creation specification are All should remain within the scope of this creation patent. BRIEF DESCRIPTION OF THE DRAWINGS The first figure is a schematic diagram of measuring the resistance between wires with two probes. The second diagram of the data analysis processing of the present invention is a third diagram of the present invention. Schematic diagram of the resistance between the four probes for measuring the wires [Description of main components] 10·backplane 20. wire 25. working electrode 30. reference electrode 40. probe 50. wire 70. reaction enzyme 77. power supply 78. relay 79. Resistance meter 80. Analysis device 81. Database comparison 82. Display result device