TW200916118A

TW200916118A - External preparation for skin

Info

Publication number: TW200916118A
Application number: TW097127333A
Authority: TW
Inventors: Tadakazu Otomo
Original assignee: Program Co Ltd Dr
Priority date: 2007-08-20
Filing date: 2008-07-18
Publication date: 2009-04-16
Also published as: JP2009046421A; WO2009025062A1

Abstract

It is intended to provide an external preparation for skin in which the moisturizing effect of the external preparation for skin containing a yeast extract is increased thereby to provide an excellent moisturizing feel to the skin. The external preparation for skin contains the following components (A) and (B): (A) a yeast extract; and (B) a liposome including one or two types of amino acids selected from the group consisting of tryptophan and histidine. By applying the external preparation for skin to the skin, a moisturizing feel can be provided to the skin. This moisturizing feel is further enhanced by blending one or more types of amino acids selected from the group consisting of valine, leucine, isoleucine, threonine, lysine, methionine and phenylalanine.

Description

200916118 九、發明說明【發明所屬之技術領域】本發明係關於含有微脂體之皮膚外用劑。【先前技術】爲給予肌膚水嫩、滋潤’傳統以來提供配合具有保濕作用之化合物之皮膚外用劑。酵母萃取物係安全性亦高，具有保濕作用之重要化合物之一。然而，依據酵母萃取物之保濕作用並不一定高，配合酵母萃取物之皮膚外用劑對於肌膚之保濕作用稱不上充分。作爲配合酵母萃取物之皮膚外用劑之先前技術，有面膜、乳霜、乳液等例（參考專利文獻1、2、3、4及5 )。另一方面’雖已知爲提升藥效成份之藥效，將藥效成份微脂體化之技術，但未知關於包含酵母萃取物之微脂體。另外’包含本發明相關化合物之胺基酸之微脂體，揭示關於皮膚外用劑之技術（例如參考專利文獻6及7 )。 [專利文獻1 ]特開2 0 0 0 — 2 2 9 8 2 7號公報 [專利文獻2]特開2002 — 326922號公報 [專利文獻3]特開2003 - 160463號公報 [專利文獻4]特開2003 - 238378號公報 [專利文獻5]特開2005 — 220084號公報 [專利文獻6 ]特開平7 — 2 8 5 8 2 7號公報 [專利文獻7 ]特開平8 - 2 0 8 4 2 3號公報 200916118 【發明內容】發明所欲解決之課題本發明係有鑑於上述情況所實施者，該目的係提高配合酵母萃取物之皮膚外用劑之保濕作用，提供給予肌膚優異之保濕感之皮膚外用劑。課題之解決手段本發明者等爲解決上述課題而努力進行硏究的結果，發現將酵母萃取物與特定的胺基酸一同微脂體化，藉由使含於皮膚外用劑中，解決上述課題，而達成完成本發明。亦即，本發明係包含下述成份（A )及（B ): (A )酵母萃取物、 (B )包含1種或2種選自色胺酸及組織胺酸所成群之胺基酸之微脂體（Liposome )之皮膚外用劑。本發明中，於上述成份（B)中，再含有1種或2種以上選自纈胺酸、白胺酸、異白胺酸、蘇胺酸、賴胺酸、甲硫胺酸及苯丙胺酸所成群之胺基酸，因爲更增強保濕作用，所以爲適合之實施型態。發明之功效使微脂體包含酵母萃取物與特定的胺基酸，藉由配合於皮膚外用劑，可得到給予肌膚優異的保濕感之皮膚外用劑。本發明之皮膚外用劑係由每日使用而發揮更優異之保濕效果。 -5- 200916118 用以實施發明之最佳型態以下係詳述關於用以實施本發明之最佳型態。作爲本發明中所使用之酵母萃取物之酵母，並無特別限制’可舉例如麵包酵母、啤酒酵母、葡萄酒酵母、清酒酵母等。作爲本發明中所使用之酵母萃取物，可使用酵母之由極性溶劑之萃取物、藉由自己消化、酸水解或酵素分解等’使酵母溶菌後過濾者，或乾燥上述溶液，自此以極性溶劑萃取者’但並不侷限於此等’作爲酵母萃取物之型態’可爲含溶劑之萃取液等，亦可爲除去溶劑之糊狀或粉末物之任一種型態皆可使用。另外，作爲萃取所使用之極性溶劑，可使用水、乙醇、1，3 _ 丁二醇、丙二醇、甘油、或此等之任意混合溶劑。酵母萃取物係可使用所萃取之粗萃取物之原本狀態，或進一步進行過濾、濃縮等作爲精製萃取物。本發明中，可使用市售者作爲酵母萃取物。作爲市售品，可舉例如 Cytocatalyzer (山川貿易股份有限公司製）、：Biodynes (GSI Creos股份有限公司製）等。上述cytocatalyzer係藉由自己消化法處理麵包酵母中之有效成份，萃取·濃縮. 無臭化之水溶性萃取物。本發明中酵母萃取物之配合量，作爲純乾燥成份’係以皮膚外用劑總量中7.5 X 1 〇·3〜1.0 質量％爲宜。本發明中，作爲必須成份，可使用一種或二種之選自色胺酸及組織胺酸所成群之胺基酸。色胺酸及組織胺酸係 -6- 200916118 雜環胺基酸（以下亦以（異）表示。）物質。本發明中’上述一種或二種之選酸所成群之胺基酸之配合量係以於皮 7·75χ1(Γ7〜10·0質量％爲宜。本發明中二種之選自色胺酸及組織胺酸所成群之合一種或二種以上選自纈胺酸、白胺酸酸、賴胺酸、及甲硫胺酸所成群之脂肪以（脂）表示。），由苯丙胺酸所成之下亦以（芳）表示。）。上述纈胺酸、、蘇胺酸、賴胺酸、甲硫胺酸及苯丙胺或2種以上選自此等群，但以全部配合等胺基酸，可更增強保濕作用。上述本組織胺酸（雜環胺基酸）、纈胺酸、白蘇胺酸、賴胺酸、甲硫胺酸（脂肪族胺 (芳香族胺基酸）之9種胺基酸，已知基酸。另外，配合上述一種或二種以上酸、異白胺酸、蘇胺酸、賴胺酸、甲硫成群之脂肪族胺基酸時，與上述一種或及組織胺酸所成群之胺基酸之合倂量係量中之7.75xl(T7〜10.0質量％爲宜。本發明中，作爲胺基酸，於必須成酸中，更配合上述脂肪族胺基酸及/或相對於1質量份之雜環胺基酸’以配合肪族胺基酸、〇· 5〜2.0質量份之芳香族，任一種皆爲已知自色胺酸及組織胺膚外用劑總量中之，除了上述一種或胺基酸以外，可配、異白胺酸、蘇胺族胺基酸（以下亦芳香族胺基酸（以白胺酸、異白胺酸酸，雖可使用1種爲宜。藉由配合此發明中之色胺酸及胺酸、異白胺酸、基酸）、苯丙胺酸作爲所謂的必須胺選自纈胺酸、白胺胺酸及苯丙胺酸所二種之選自色胺酸以於皮膚外用劑總份之上述雜環胺基芳香族胺基酸時， 6〜1 〇質量份之脂胺基酸之範圍爲宜 -7- 200916118 本發明之皮膚外用劑中，使酵母萃取物與上述胺基酸包含於以磷脂質作爲結構成份微脂體，含於皮膚外用劑。由微脂體化，明顯地發揮由配合酵母萃取物與上述胺基酸所得之保濕效果。作爲微脂體化所使用之磷脂質，只要係化粧料、醫藥部外用品、醫藥品等所使用者即可，並無特別限制。另外 ’本發明中所謂磷脂質係包含天然磷脂質、氫化磷脂質等之磷脂質衍生物、合成磷脂質等之槪念者。作爲磷脂質，可舉例如磷脂醯膽鹼（Phosphatidyl Choline )、磷脂醯乙醇胺、磷脂醯絲胺酸、磷脂醯肌醇、神經磷脂（ sphingomyelin)等之天然磷脂質、以氫飽和天然磷脂質中之不飽和碳鏈之氫化磷脂質、二油醯磷脂醯膽鹼等之合成磷脂質等。本發明中係以使用大豆磷脂質、蛋黃磷脂質等之磷脂質、氫化大豆磷脂質、氫化蛋黃磷脂質等之氫化磷脂質、合成磷脂質爲宜，以氫化磷脂質尤佳，以氫化大豆磷脂質更好。磷脂質係可單獨使用其1種，或亦可適當組合2種以上使用。以安定化微脂體爲目的，可添加膽固醇、葡萄糖、高級醇、非離子性界面活性劑、離子性界面活性劑等之安定化劑。上述微脂體之安定化劑係例如於調製上述微脂體時，與磷脂質等一同混合，可作爲微脂體之結構成份之一。作爲上述非離子性界面活性劑，可舉例如山梨糖醇酐脂肪酸酯類（山梨糖醇酐單硬脂酸酯、倍半油酸山梨糖醇 -8- 200916118 二醇生物糖醇糖醇山梨油單酸酯醚類 POE 以下等）篦麻苷等配合，並法、，若。之胺基合。機（處理酐酯等）、甘油脂肪酸類（單硬脂酸甘油酯等）、丙脂肪酸酯類（單硬脂酸丙二醇酯等）、硬化蓖麻油衍、甘油烷基醚、聚環氧乙烷（以下稱爲p〇E。）山梨酐脂肪酸酯類（POE山梨糖醇酐單油酸酯、POE山梨酐單硬脂酸酯等）、POE山梨糖醇脂肪酸酯類（POE 糖醇單月桂酸酯等）、POE甘油脂肪酸酯類（POE甘異硬脂酸酯等）、聚乙二醇（以下稱爲PEG。）脂肪類（PEG單油酸酯、PEG二硬脂酸酯等）、POE烷基 (POE2-辛基十二烷基醚等）、P〇E烷基苯基醚類（壬基苯基醚等）、Plur〇nic型類、POE·聚環氧丙烷（稱爲POP。）烷基醚類（Ρ0Ε·Ρ0Ρ2—癸基十四烷基醚、T e t r ο n i c類、Ρ Ο Ε鹿麻油·硬化篤麻油衍生物（Ρ 0 Ε 油、POE硬化箆麻油等）、蔗糖脂肪酸酯、烷基葡糖。非離子性界面活性劑係可任意選擇1種或2種以上〇關於調製包含酵母萃取物與上述胺基酸之微脂體無特別限定，可以超音波法、勻化法、薄膜法、注入界面活性劑除去法等已知方法調製。例如於磷脂質中需要時，加熱混合、溶解多元醇、膽固醇等之安化劑後，將酵母萃取物及胺基酸，例如以酵母萃取物液及酸液之形態添加，攪拌混合，於其中加水，再攪拌混之後，以高壓攪拌器（例如均質流細胞粉碎 microfluidizer)之高壓乳化機或 APV勻質化器等）使形成微脂體。接著’加溫時，冷卻至室溫，得到微脂 200916118 體液。另外，高壓攪拌器處理之混合物爲分散液時，藉由處理前步驟之高速攪拌（例如以勻化器之3 000rpm以上之攪拌速度）等以提高分散爲宜。另外，於上述舉例的製法中，以磷脂質等成份，調製酵母萃取物及/或胺基酸除外之微脂體，接著，進而將該微脂體與上述除外成份（酵母萃取物及/或胺基酸），因應需要之其他包含成份混合、攪拌，以高速攪拌爲宜等，亦可與上述同樣地調製。另外，調製未包含充分量之酵母萃取物及/或胺基酸之微脂體 '或完全不包含酵母萃取物及/或胺基酸之微脂體，接著，混合酵母萃取物及/或胺基酸，使成爲必要量，亦可與上述同樣地調製。關於藉由此等方法調製包含酵母萃取物及胺基酸之微脂體，例如可利用市售之未包含酵母萃取物及/或胺基酸之微脂體，藉由利用其而可有效率地得到品質更安定之包含高濃度之酵母萃取物及/或胺基酸之微脂體。作爲市售之上述不包含酵母萃取物及/或胺基酸之微脂體例，可舉例如NIKKOL Aquasome BH、同LA (以上爲曰光Chemicals公司製）等。本發明中雖可直接使用此等市售品，但當提高微脂體之濃度等，調製本發明之皮膚外用劑，變換成容易使用型態亦爲有效率的方法。微脂體係由磷脂質之二分子膜之一層或多層所形成之球狀小胞體，成爲酵母萃取物及/或胺基酸收納於磷脂質的膜中或小胞體內之狀態（包含）。微脂體之粒徑係以平均粒徑爲30〜200nm爲宜。其中以40〜100 nm尤佳。平 -10- 200916118 均粒徑超過20 Onm時，對肌膚的滲透化差，所以不宜。另外，微脂體之粒徑係使用濃厚系粒徑分析器FPAR - 1〇〇〇 (大塚電子股份有限公司製，光散射光度計）於室溫下測定。微脂體一般係以水分散液存在，爲水所任意稀釋。皮膚外用劑中，任意爲皮膚外用劑中的水所稀釋，但本發明中所謂的微脂體係包含存在於皮膚外用劑中所任意稀釋之微脂體水分散液之槪念。本發明中，包含酵母萃取物及上述胺基酸於微脂體，含有於皮膚外用劑中，但作爲上述微脂體，不僅包含酵母萃取物與上述胺基酸混合物者之微脂體，皮膚外用劑中之微脂體內存在規定的酵母萃取物與上述胺基酸。例如亦可爲酵母萃取物及上述胺基酸分別包含於各不同的微脂體，含有各個微脂體於皮膚外用劑中。另外，關於使用胺基酸混合物，不僅包含所混合之胺基酸之微脂體，亦可將包含各一種的胺基酸或任意種類所混合之胺基酸之微脂體，分別含有於皮膚外用劑中。另外，關於酵母萃取物，使用二種以上時係與胺基酸時相同。本發明中，作爲含有全部此等微脂體之型態者，具有如申請專利範圍第1項及第2項之表現。亦即’例如申請專利範圍第1項中係含有包含下述成份（A )： (A)酵母萃取物之微脂體，及包含下述成份（b)： (B ) —種或二種選自色胺酸及組織胺酸所成群之胺基酸之微脂體之皮膚外用劑所包含者。本發明中，就保濕 -11 - 200916118 效果上’以包含酵母萃取物及上述胺基酸混合物者之微脂體爲宜。關於使包含酵母萃取物及胺基酸之微脂體，含有於皮膚外用劑中’亦可配合不包含酵母萃取物及胺基酸，或未包含規定量之酵母萃取物及胺基酸之微脂體，以及酵母萃取物及胺基酸作爲皮膚外用劑處方之一種成份，調製皮膚外用劑時’使形成包含規定量酵母萃取物及胺基酸之微脂體亦無妨。本發明之皮膚外用劑係於不損及本發明功效之範圍而可適當地配合上述成份以外之通常化粧品、醫藥部外用品、醫藥品等之皮膚外用劑所使用之其他成份。作爲上述任意配合成份，記載著成份中所含物質爲複數成份，另外，亦包含與上述部份重疊之記載，可舉例如油份、界面活性劑、保濕劑、多元醇、增黏劑、水溶性高分子、被膜形成劑、非水溶性高分子、油膠化劑'高分子乳膠、粉末、顏料、染料、沈澱色料、低級醇、糖類、紫外線吸收劑、上述以外之胺基酸類、維生素類、美白劑、皮膚賦活劑、促進血液循環劑、抗脂漏劑、抗發炎劑（消炎劑）之藥劑、植物萃取物、有機酸、有機胺、金屬離子封鎖劑、pH調整劑、抗氧化劑、抗菌劑（防腐殺菌劑）、收斂劑、清涼劑、香料、水等。上述任意配合成份中，作爲油份之具體例，可舉例如硬化油、木蠟、可可脂、硬化箆麻油、橄欖油、篦麻油、夏威夷火山豆油' 月見草油等之油脂；蜂蠟、羊毛脂、鯨 -12- 200916118 油、小燭樹蠟'巴西棕櫚蠟、蟲蠟、荷荷芭蠟、荷荷芭油、液狀羊毛脂等之蠟類；固體鏈烷烴、純地蠟、微晶紫躐、聚乙嫌蠟、流動鏈院煙、三十碳烷、聚丁嫌、揮發性烴等之烴油；棕櫚酸十六烷酯、肉宣蔻酸異丙酯、棕櫚酸異丙酯、2-乙基己酸十六烷酯、肉宣蔻酸辛基十二烷酯、硬脂酸辛酯、棕櫚酸辛酯、己二酸二異丙酯、癸二酸2一乙基己酯、二癸酸新戊二醇酯、三肉宣蓮酸甘油酯、三一 2—乙基己酸甘油酯、三辛酸甘油酯、三異硬脂酸甘油酯 '二異硬脂酸二甘油酯、三異硬脂酸二甘油酯、四2-乙基己酸季戊四醇酯、二辛酸季戊四醇酯、三2—乙基己酸三羥甲基丙烷、三異硬脂酸三羥甲基丙烷、蘋果酸二異硬脂醯等之酯油；硬脂酸、棕櫚酸、肉宣蔻酸、12 -羥基硬脂酸、二十二烷酸、異硬脂酸、油酸等之高級脂肪酸；十六烷醇、硬脂醇、倍半硬脂醇、二十二烷醇、異硬脂醇、油醇、辛基十二烷醇等之高級醇、二甲基聚矽氧烷、甲基苯基聚矽氧烷、二苯基聚矽氧烷等之鏈狀聚矽氧烷、八甲基環四矽氧烷、十甲基環五矽氧烷、十二甲基環六矽氧烷等之環狀聚矽氧烷、胺基改性聚矽氧烷、烷基改性聚矽氧烷、氟改性聚矽氧烷等之改性聚矽氧烷等。油份係可任意選擇1種或2種以上配合。上述任意配合成份中，作爲多元醇之具體例’可舉例如甘油、1，3 — 丁二醇（1,3 - BG) 、1，2—戊二醇、赤蘚醇、山梨糖醇、木糖醇、麥芽糖醇、丙二醇、二丙二醇（ D P G )、二甘油等。多元醇係可任意選擇1種或2種以上 -13- 200916118 配合。上述任意配合成份中，作爲保濕劑之具體例，可舉例如乳酸鹽（鈉等）、吡咯烷酮羧酸鈉（p c A鈉）、尿素等。保濕劑係可任意選擇1種或2種以上配合。上述任意配合成份中，作爲水溶性高分子之具體例，可舉例如瓜爾豆膠、搵桴子（quince seed )、鹿角菜膠、半乳聚糖、阿拉伯膠、果膠、甘露聚糖、澱粉、普路蘭、三仙膠、卡德蘭膠（Curdlan )、甲基纖維素、羥乙基纖維素、羧甲基纖維素、甲基羥丙基纖維素、硫酸軟骨素（ Chondroitin sulfate )、硫酸皮膚素（dermatan sulfate ) 、肝醣、硫酸肝素（heparan sulfate )、透明質酸、透明質酸鈉、黃蓍膠、硫酸角質素（keratan sulfate )、軟骨素、硫酸黏液素（mucoitin sulfate)、經乙基瓜爾豆膠、羧甲基瓜爾豆膠、葡聚糖、硫酸角質（Kerato sulfate )、刺槐豆膠、琥珀醯葡聚糖（sue cino glue an )、卡洛寧酸、甲殼質、殼聚糖、羧甲基甲殼質、瓊脂、聚乙烯醇、聚乙烯吡咯烷酮、羧乙烯聚合物、聚丙烯酸鈉、PEG等。水溶性高分子係可任意選擇1種或2種以上配合。本發明之皮膚外用劑係配合上述成份，依據常法而$ 調製。另外，如上所述，調製皮膚外用劑時，亦可與調製皮膚外用劑的同時，使微脂體形成。作爲本發明之皮膚外用劑之型態，雖無特別限定，可舉例如乳液、乳霜、化粧水、面膜等。另外，作爲劑型，雖無特別限定，可舉例如凝膠、分散液、軟膏、外用液、 -14- 200916118 乳霜等，尤其就保濕效果上，以凝膠系爲宜。【實施方式】實施例以下係舉實施例具體地說明本發明。配合量係未特別記載者係質量％。說明實施例之前，先說明關於本發明使用之效果試驗方法。保濕效果藉由1 〇位具有化粧品使用感官能評估經驗之女性調查員，進行效果測試。調查員係洗臉後塗佈適量試驗品於臉上，以官能評估塗佈2小時後之保濕感。 (評估基準） ◎:強烈地感到保濕〇：感到保濕 △:些微感到保濕 X :未感到保濕 [調製胺基酸液] 混合表1所揭示之成份，調製胺基酸液A〜D。 -15- 200916118 表 1 胺某酸液（質量％) A B C D 色胺酸(異） 3.1 1.55 0.05 組織胺酸(異） 3.1 1.55 0.25 纈胺酸(脂） 0.50 白胺酸(脂） _ - 0.70 異白胺酸(脂） _ - 0.30 蘇胺酸(脂） - 0.30 賴胺酸(脂）緯 - 0.60 甲硫胺酸(脂） - 0.10 苯丙胺酸(芳） - 0.30 1,3-BG 15.0 15.0 15.0 15.0 精製水剩餘量剩餘量剩餘量剩餘量 [調製酵母萃取物液] 調製由表2所揭示之成份所形成之酵母萃取物液A ( 作爲酵母萃取物純乾燥成份爲1 . 5質量％ )。調製係使用 Cytocatalyzer BG30 (山川貿易股份有限公司製）。表 2 酵母萃取物液Α ί筲，％、酵母萃取物 ----—一JL—兵里 /υ 7 1 . 5 1,3-BG ^00 精製水 ——一__ 剩餘量 [調製微脂體] 使用表3所示之成份，以微脂體Α〜G (配合量合計爲100 A〜D係袠1所示者，酵 Γ巡万法製 ''告。表3使用之各成份比率係以質量％ 33 質量％ )表示。另外，胺基酸液 ' 16- 200916118 母萃取物液A係表2所示者。以5 0 °C混合氫化大豆磷脂質、甘油、及部份D P G後，添加D P G剩餘部份及膽固醇之混合物，以5 0 °C加熱溶解。之後，添加酵母萃取物液A 及該胺基酸液，攪拌混合。之後，加入精製水成爲1 00質量％，混合攪拌。最後，以高壓乳化機（「均質流細胞粉碎機（micro fluidizer)」美國 Micro fluidics 公司製）處理，得到微脂體。所得之微脂體之平均粒徑爲4 5 nm。另外，表3係揭示配合酵母萃取物液A及胺基酸液A或B 之非微脂體組成物之非微脂體A及B之成份。此非微脂體係混合各成份而調製。表 3 微脂體（質量％) 非微左 (質量旨體 [%) A B C D E F G A B 氫化大豆磷脂質(注） 2.0 2.0 2.0 2.0 2.0 2.0 2.0 . _ 甘油 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 DPG 12.0 12.0 12.0 12.0 12.0 12.0 12.0 12.0 12.0 膽固醇 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 酵母萃取物液A 20.0 20.0 40.0 _ 20.0 20.0 20.0 20.0 胺基酸液A 5.0 — 10.0 • 一 . 5.0 胺基酸液B • 5.0 • - 10.0 _ 琴祕 5.0 胺基酸液C _ 5.0 _ 胺基酸液D _ _ _ _ _ 5.0 精製水剩餘剩餘剩餘剩餘剩餘剩餘剩餘剩餘剩餘量量量量量量量暈量 (注）NIKKOL Lecinol S— 10 (日光 Chemicals 公司製） -17- 200916118 [調製皮膚外用劑] [實施例1〜4，比較例1〜5] 皮膚外用劑（凝膠乳霜）係加入表4所示規定量之成份，作爲凝膠製劑。另外，微脂體A〜G及非微脂體A、 B係表3所示者。表 4 實方 _ 比較例實方 _ 1 2 1 2 3 4 5 3 4 甘油 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 1,3-BG 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 羧乙烯基聚合物 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 丙烯酸•甲基丙烯酸烷基酯共聚物 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 苯氧基乙酉爭 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 1,2-己二酸 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 氫氧化鉀 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 微脂體A 25.0 _ 微脂體B 25.0 _ - _ 两 _ _ 微脂體C _ 25.0 _ _ 一 - _ • 微脂體D . . 25.0 _ _ _ . _ 微脂體E - - 齡 25.0 _ . 一 _ 微脂體F - . - _ _ _ 25.0 _ 微脂體G - - - _ _ _ . _ 25.0 非微脂體A • - _ 25.0 _ , 非微脂體B - - - _ _ 25.0 _ - 精製水剩餘量剩餘量剩餘量剩餘量剩餘量剩餘量剩餘量剩餘量剩餘量關於實施例1〜4，比較例1〜5之皮膚外用劑（凝膠 -18- 200916118 乳霜），進行效果試驗’該評估結果如表5所示。表 5 保濕感（人數） ◎ 〇 Δ X 實施例 1 6 4 0 0 實施例 2 6 4 0 0 比較例 1 0 3 7 0 比較例 2 0 3 7 0 比較例 3 0 3 7 0 比較例 4 0 4 5 1 比較例 5 0 4 5 1 實施例 3 8 2 0 0 實施例 4 10 0 0 0 由表5可知配合包含酵母萃取物及雜環胺基酸之色胺酸及/或組織胺酸之微脂體之皮膚外用劑，與配合僅包含酵母萃取物之微脂體之皮膚外用劑（比較例1)、配合包含色胺酸或組織胺酸之微脂體之皮膚外用劑（比較例2、3 )、及配合酵母萃取物及色胺酸或組織胺酸之非微脂體物之皮膚外用劑（比較例4、5 )比較，相乘地賦予肌膚優異的保濕感。另外，可知於作爲胺基酸之由色胺酸及/或組織胺酸所形成之雜環胺基酸，再配合纈胺酸、白胺酸、異白胺酸、蘇胺酸、賴胺酸、及甲硫胺酸所成之脂肪族胺基酸以及由苯丙胺酸所成之芳香族胺基酸之皮膚外用劑（實施例4 )係對肌膚之保濕感更加增強。 [實施例5〜8 ] -19- 200916118 以下係另外表示本發明之由表6之組成所成之皮膚外用劑之實施例（實施例5〜8 (凝膠乳霜））。另外，製造係依據實施例1〜4之方法進行。表6配合之微脂體Η〜K 係與表3者同樣地調製，使用表7所示者。另外，表7中之胺基酸液Α〜D係表1所示者，酵母萃取物液Α係表2 所示者。表 6 實施例（質量％ ) 5 6 7 8 甘油 5.0 5.0 5.0 5.0 1，3 -BG 8.0 8.0 8.0 8.0 羧乙烯基聚合物 0.6 0.6 0.6 0.6 丙烯酸•甲基丙烯酸烷基酯共聚物 0.2 0.2 0.2 0.2 苯氧基乙醇 0.5 0.5 0.5 0.5 1，2-己二醇 0.05 0.05 0.05 0.05 氫氧化鉀 0.3 0.3 0.3 0.3 微脂體Η 25.0 - - _ 微脂體I 25.0 . _ 微脂體J _ 25.0 編微脂體Κ - _ 25.0 精製水剩餘量剩餘量剩餘量剩餘量 -20- 200916118 表 Ί 微脂體（質量％ ) Η I J Κ 氫化大豆磷脂質（注） 2.0 2.0 2.0 2.0 甘油 20.0 20.0 20.0 20.0 DPG 12.0 12.0 12.0 12.0 膽固醇 0.2 0.2 0.2 0.2 酵母萃取物液A 20.0 20.0 20.0 20.0 胺基酸液A 0.00 1 . 胺基酸液B 0.1 _ • 胺基酸液C __ 0.0001 胺基酸液D . 0.0 1 精製水剩餘量剩餘量剩餘量剩餘量 (注）NIKKOL Lecinol S - 10 (曰光 Chemicals 公司製） [實施例9〜17] 以下係另外表不本發明之表8之組成所成之皮膚外用劑之實施例（實施例9〜1 7 (凝膠乳霜））。另外，製造係依據實施例1〜4之方法進行。表8配合之微脂體A、B 、L〜S係使用表3所示者及同樣地調製之表9所示者。另外’表9中之胺基酸液E〜L係表10所示者，酵母萃取物液A係表2所示者。 -21 - 200916118 表 8 實施例（質量％) 9 10 11 12 13 14 15 16 17 甘油 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 1,3-BG 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 羧乙烯基聚合物 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 丙烯酸•甲基丙烯酸烷基酯共聚物 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 苯氧基乙醇 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 1,2-己二醇 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 氫氧化鉀 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 微脂體A 12.5 微脂體B 12.5 微脂體L 25.0 _ _ _ _ . _ _ 微脂體Μ - 25.0 _ • _ _ _ • 微脂體Ν . _ 25.0 _ _ . _ _ 微脂體〇 _ _ . 25.0 _ _ — _ _ 微脂體Ρ - _ _ • 25.0 - • 微脂體Q _ _ • _ 25.0 _ _ _ 微脂體R _ - _ • 25.0 微脂體S - - 一 - _ _ _ 25.0 _ 精製水剩餘剩餘剩餘剩餘剩餘剩餘剩餘剩餘剩餘量量量量量量里量量 -22- 200916118 表 9 微脂體（質量％ ) L Μ Ν 0 Ρ 0 R S 氫化大豆磷脂質（注） 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 甘油 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 DPG 12.0 12.0 12.0 12.0 12.0 12.0 12.0 12.0 膽固醇 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 酵母萃取物液A 2.0 5.0 30.0 10.0 15.0 10.0 15.0 20.0 胺基酸液E 0.5 胺基酸液F 0.05 _ _ _ 胺基酸液G 0.003 _ _ . _ 胺基酸液Η • • 0.0003 • 胺基酸液I • • . 0.05 _ __ • 胺基酸液J _ • • _ 0.0005 • _ 胺基酸液Κ • • _ 0.03 胺基酸液L • 釋 • 0.005 精製水剩餘剩餘剩餘剩餘量剩餘剩餘剩餘剩餘量量量量量量量 (注）NIKKOL Lecinol S — 10 (日光 Chemicals 公司製） -23- 200916118 表 ι〇胺基酸液（質量°/。） E F G Η I J Κ L 色胺酸（異） 0.05 0.35 0.26 0.14 0.1 0.35 . _ 組織胺酸（異） 0.3 _ 1.34 0.2 0.17 . 0.35 1.3 纈胺酸（脂） 2.75 - - 0.4 0.5 _ 白胺酸（脂） - 華 _ 0.4 0.5 2.0 _ 異白胺酸（脂） _ - - 0.34 0.5 - 2.45 _ 蘇胺酸（脂） _ _ _ 0.3 0.4 0.45 _ 賴胺酸（脂） 2.35 _ 0.3 0.4 一 _ 甲硫胺酸（脂） - 0.4 _ 0.3 0.4 _ - 苯丙胺酸（芳） - - 1.5 0.72 0.13 0.3 0.3 1.8 1,3-BG 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 精製水剩餘量剩餘量剩餘量剩餘量剩餘量剩餘量剩餘量剩餘量 [實施例18〜20] 以下係另外表示本發明之皮膚外用劑。另外，使用的胺基酸液及酵母萃取物液係如下調製。 [調製胺基酸] 混合表1 1所揭示成份，調製胺基酸Μ、N。 -24- 200916118 表 11 胺基酸（質量％) Μ Ν 色胺酸（異） 16.0 1.6 組織胺酸（異） 84.0 8.0 纈胺酸（脂） - 16.1 白胺酸（脂） - 22.6 異白胺酸（脂） - 9.7 蘇胺酸（脂） - 9.7 賴胺酸（脂） - 19.4 甲硫胺酸（脂） - 3.2 苯丙胺酸（芳） - 9.7 [調製酵母萃取物液] 混合由表1 2所揭示之成份，調製作爲酵母萃取物純乾燥成份爲〇 · 4質量％ ( B ) 、2.0質量％ ( C ) 、4 · 0質量 % ( D )之酵母萃取物液B〜D。另外，此等酵母萃取物液係使用 Cytocatalyzer BG30 (山川貿易股份有限公司製）調製。表 12 酵母萃取物液（質量％ ) Β C D 酵母萃取物 0.4 2.0 4.0 1 ,3 -BG 8.0 40.0 80.0 精製水 9 1.6 5 8.0 16.0 實施例1 8化粧水 -25- 200916118 成份200916118 IX. Description of the Invention [Technical Field of the Invention] The present invention relates to a skin external preparation containing a liposome. [Prior Art] In order to impart moisturizing and moisturizing to the skin, a skin external preparation containing a compound having a moisturizing effect has been conventionally provided. The yeast extract is also one of the important compounds with high safety and moisturizing effect. However, depending on the moisturizing action of the yeast extract, the skin external application agent combined with the yeast extract is not sufficient for moisturizing the skin. As a prior art of a skin external preparation for yeast extract, there are examples of masks, creams, lotions, and the like (refer to Patent Documents 1, 2, 3, 4, and 5). On the other hand, although it is known that the efficacy of the medicinal ingredient is improved, the medicinal effect is a technique of micro-lipidation, but the micro-lipid containing the yeast extract is not known. Further, the microlipid of the amino acid containing the compound of the present invention discloses a technique for external preparation for skin (for example, refer to Patent Documents 6 and 7). [Patent Document 1] JP-A-2003-160463 [Patent Document 3] JP-A-2003-160463 (Patent Document 4) [Patent Document 5] JP-A-2005-220084 [Patent Document 6] Japanese Laid-Open Patent Publication No. Hei No. Hei No. Hei. SUMMARY OF THE INVENTION PROBLEM TO BE SOLVED BY THE INVENTION The present invention has been made in view of the above circumstances, and it is an object of the present invention to improve the moisturizing action of a skin external preparation which is combined with a yeast extract, and to provide a skin external application for giving an excellent moisturizing feeling to the skin. Agent. In order to solve the above problems, the inventors of the present invention have found that the yeast extract is lipolyzed together with a specific amino acid, and the above-mentioned problem is solved by being contained in an external preparation for skin. And the completion of the present invention is achieved. That is, the present invention comprises the following components (A) and (B): (A) a yeast extract, (B) comprising one or two amino acids selected from the group consisting of tryptophan and histidine. A skin external preparation of liposome. In the present invention, one or more of the above components (B) are further selected from the group consisting of lysine, leucine, isoleucine, threonine, lysine, methionine and phenylalanine. The group of amino acids is a suitable embodiment because it enhances the moisturizing effect. EFFECT OF THE INVENTION The liposome contains a yeast extract and a specific amino acid, and by blending with an external preparation for skin, an external preparation for skin which gives an excellent moisturizing feeling to the skin can be obtained. The skin external preparation of the present invention exerts a more excellent moisturizing effect by daily use. -5- 200916118 BEST MODE FOR CARRYING OUT THE INVENTION The following is a detailed description of the best mode for carrying out the invention. The yeast of the yeast extract used in the present invention is not particularly limited, and examples thereof include baker's yeast, brewer's yeast, wine yeast, and sake yeast. As the yeast extract used in the present invention, yeast extracts of a polar solvent, by self-digestion, acid hydrolysis or enzymatic decomposition, etc., can be used to filter the yeast after lysing, or the solution is dried, and then the polarity is used. The solvent extractor 'but is not limited to these types as the yeast extract' may be a solvent-containing extract or the like, or may be used in any form of a paste-like or powder-containing solvent. Further, as the polar solvent used for the extraction, water, ethanol, 1,3-butanediol, propylene glycol, glycerin, or any mixed solvent of these may be used. The yeast extract may be used as a purified extract by using the original state of the extracted crude extract, or further filtering, concentrating, or the like. In the present invention, a commercially available person can be used as the yeast extract. As a commercial item, Cytocatalyzer (made by Yamakawa Trading Co., Ltd.), Biodynes (made by GSI Creos Co., Ltd.), etc. are mentioned, for example. The above cytocatalyzer is used to treat the active ingredients in baker's yeast by its own digestion method, extracting and concentrating. The odorless water-soluble extract. The blending amount of the yeast extract in the present invention is preferably 7.5 X 1 〇·3 to 1.0% by mass based on the total amount of the external preparation for skin. In the present invention, as an essential component, one or two kinds of amino acids selected from the group consisting of tryptophan and histidine can be used. Tryptophan and histamine -6- 200916118 Heterocyclic amino acid (hereinafter also indicated by (di)). In the present invention, the amount of the amino acid in the group of the above-mentioned one or two kinds of selected acids is preferably 7·75χ1 (Γ7 to 10·0% by mass). In the present invention, the two kinds are selected from the group of tryptamines. One or more of the acid and histidine groups are selected from the group consisting of lysine, leucine, lysine, and methionine, and the fat is represented by (fat).), amphetamine The acid is also expressed as (fang). ). The above-mentioned lysine, sulphate, lysine, methionine, and amphetamine may be selected from these groups, but the amino acid such as all may be added to enhance the moisturizing effect. The above 9 kinds of amino acids of histamine (heterocyclic amino acid), valine acid, methionine, lysine, and methionine (aliphatic amine) are known. Further, in combination with one or more of the above-mentioned aliphatic, amino acid, threonine, lysine, and methylthio group, the aliphatic amino acid is grouped with the above-mentioned one or a histidine 7.75xl of the combined amount of the amino acid (T7~10.0% by mass is preferable. In the present invention, as the amino acid, it is necessary to form an acid, and more preferably the above aliphatic amino acid and/or relative 1 part by mass of the heterocyclic amino acid' is blended with an aliphatic amino acid, and 5 to 2.0 parts by mass of the aromatic, each of which is known to be a total amount of the external agent for tryptophan and histamine. In addition to the above-mentioned one or amino acid, it can be combined with iso-leucine and a sulphamine-based acid (hereinafter also an aromatic amino acid (with leucine and isoleucine, although one type can be used) Preferably, by combining the tryptophanic acid and the amine acid, the isoleucine, the base acid, and the phenylalanine in the invention, the so-called essential amine is selected from the group consisting of guanamine. And lysine and phenylalanine are selected from the group consisting of tryptophan and the above-mentioned heterocyclic amino aromatic amino acid as a total of the external preparation for skin, and the range of 6 to 1 part by mass of the fatty amino acid In the external preparation for skin of the present invention, the yeast extract and the amino acid are contained in a liposome containing a phospholipid as a structural component, and are contained in an external preparation for skin. The moisturizing effect obtained by blending the yeast extract with the above-mentioned amino acid is not particularly limited as long as it is a user of a cosmetic, a medical product, or a pharmaceutical product. In the present invention, the phospholipid system includes a phospholipid derivative such as a natural phospholipid or a hydrogenated phospholipid, and a synthetic phospholipid. The phospholipid may, for example, be a phospholipid choline or a phospholipid. a natural phospholipid such as ethanolamine, phospholipid lysine, phospholipid osmolar, sphingomyelin, hydrogenated phospholipid which is saturated with hydrogen in the unsaturated carbon chain of natural phospholipid, diterpenoid phospholipid In the present invention, it is preferred to use a phospholipid such as soybean phospholipid or egg yolk phospholipid, a hydrogenated phospholipid such as hydrogenated soybean phospholipid or hydrogenated egg yolk phospholipid, or a synthetic phospholipid. The hydrogenated phospholipid is particularly preferred, and the hydrogenated soybean phospholipid is more preferable. The phospholipid system may be used alone or in combination of two or more. In order to stabilize the liposome, cholesterol, glucose, and advanced may be added. A stabilizer for an alcohol, a nonionic surfactant, an ionic surfactant, etc. The stabilizer for the above-mentioned liposome is mixed with a phospholipid or the like, for example, when preparing the above-mentioned liposome, and can be used as a liposome. One of the structural components. As the nonionic surfactant, for example, sorbitan fatty acid esters (sorbitan monostearate, sesquioleate sorbitol-8-200916118 diol organism) Sugar alcohol sugar sorbitol oil monoester ether POE, etc.) ricin and other complexes, and, if,. The amine combination. Machine (treatment of anhydride esters, etc.), glycerin fatty acids (such as glyceryl monostearate), propyl fatty acid esters (such as propylene glycol monostearate), hardened castor oil, glyceryl alkyl ether, polyethylene oxide (hereinafter referred to as p〇E.) Sorbitan fatty acid esters (POE sorbitan monooleate, POE sorbitan monostearate, etc.), POE sorbitol fatty acid esters (POE sugar alcohol monolauric acid) Ester, etc., POE glycerol fatty acid ester (POE, etc.), polyethylene glycol (hereinafter referred to as PEG), fat (PEG monooleate, PEG distearate, etc.), POE An alkyl group (such as POE2-octyldodecyl ether), a P〇E alkylphenyl ether (such as nonylphenyl ether), a Plur〇nic type, and a POE-polypropylene oxide (referred to as POP). ) alkyl ethers (Ρ0Ε·Ρ0Ρ2-decyltetradecyl ether, T etr ο nic, Ρ Ε Ε 麻 · · 笃笃笃笃笃笃笃、、、、、、、、、、、、、、、、、、、、、、、、、、 An acid ester or an alkyl gluconate. The nonionic surfactant may be arbitrarily selected from one or more hydrazines. The micro-lipid of the basal acid is not particularly limited, and can be prepared by a known method such as an ultrasonic method, a homogenization method, a thin film method, or a surfactant removal method. For example, when necessary, the mixture is heated and mixed to dissolve the polyol and cholesterol. After the stabilizer is added, the yeast extract and the amino acid are added, for example, in the form of a yeast extract liquid and an acid solution, stirred and mixed, water is added thereto, and the mixture is stirred and mixed, followed by a high pressure stirrer (for example, homogenized flow cells). A microfluidizer or an APV homogenizer is pulverized to form a liposome. Then, when heating, it was cooled to room temperature to obtain a lipohydrate 200916118 body fluid. Further, when the mixture treated by the high-pressure agitator is a dispersion, it is preferred to increase the dispersion by high-speed stirring (e.g., a stirring speed of 3 000 rpm or more of the homogenizer) in the previous step of the treatment. Further, in the above-described production method, a yeast extract and/or a liposome other than an amino acid are prepared by a component such as a phospholipid, and then the liposome and the above-mentioned excipient (yeast extract and/or The amino acid is prepared by mixing and stirring other components as required, stirring at a high speed, or the like, or in the same manner as described above. In addition, a microlipid which does not contain a sufficient amount of yeast extract and/or amino acid or a liposome which does not contain yeast extract and/or amino acid at all, followed by mixing yeast extract and/or amine The base acid can be prepared in the same amount as described above. By modulating a liposome containing a yeast extract and an amino acid by such a method, for example, a commercially available liposome containing no yeast extract and/or an amino acid can be used, and it can be utilized by using it. A more stable, highly concentrated yeast extract and/or amino acid liposome is obtained. Examples of the above-mentioned microlipids which do not contain a yeast extract and/or an amino acid are, for example, NIKKOL Aquasome BH, and LA (above, Twilight Chemicals). In the present invention, such a commercially available product can be used as it is. However, it is also effective to prepare the skin external preparation of the present invention by increasing the concentration of the liposome or the like and converting it into an easy-to-use form. The microlipid system is a spherical small cell body formed of one or more layers of a phospholipid bilayer membrane, and is in a state (inclusive) in which a yeast extract and/or an amino acid is contained in a membrane of a phospholipid or a small cell. The particle size of the liposome is preferably 30 to 200 nm in average particle diameter. Among them, 40 to 100 nm is particularly preferred. Flat -10- 200916118 When the average particle size exceeds 20 Onm, the penetration of the skin is poor, so it is not suitable. Further, the particle size of the liposome was measured at room temperature using a thick particle size analyzer FPAR-1® (manufactured by Otsuka Electronics Co., Ltd., light scattering photometer). The microlipids are generally present as an aqueous dispersion and are optionally diluted with water. The skin external preparation is diluted with water in the external preparation for skin. However, the so-called microlipid system of the present invention contains a micro-lipid aqueous dispersion which is arbitrarily diluted in the external preparation for skin. In the present invention, the yeast extract and the amino acid are contained in the liposome, and are contained in the external preparation for skin, but the microlipid contains not only the liposome of the mixture of the yeast extract and the amino acid, but also the skin. A prescribed yeast extract and the above amino acid are present in the liposome in the external preparation. For example, the yeast extract and the above-mentioned amino acid may be contained in different liposomes, and each of the liposomes may be contained in an external preparation for skin. Further, regarding the use of the amino acid mixture, not only the micro-lipid of the mixed amino acid but also the amino acid containing each of the amino acids or the amino acid of any kind mixed may be contained in the skin. In the external preparation. Further, when two or more kinds of yeast extracts are used, they are the same as those of the amino acid. In the present invention, as a type containing all such liposomes, there are performances as in the first and second aspects of the patent application. That is, for example, the first item of the patent application includes the following components (A): (A) the yeast extract of the liposome, and the following components (b): (B) one or two A skin external preparation containing a liposome of a group of amino acids of tryptophan and histidine. In the present invention, it is preferred that the moisturizing -11 - 200916118 is a liposome containing a yeast extract and a mixture of the above amino acids. The microlipid containing the yeast extract and the amino acid, which is contained in the external preparation for skin, may be combined with no yeast extract and amino acid, or may not contain a predetermined amount of yeast extract and amino acid. The fat body, as well as the yeast extract and the amino acid are used as a component of the skin external preparation, and when the skin external preparation is prepared, it is also possible to form a liposome containing a predetermined amount of the yeast extract and the amino acid. The external preparation for skin of the present invention can be appropriately blended with other components used for external preparations for skin other than the above-mentioned ingredients, such as general cosmetics, medical external products, and pharmaceuticals, without departing from the scope of the present invention. The above-mentioned optional component contains a plurality of components contained in the component, and includes a description overlapping with the above-mentioned components, and examples thereof include an oil component, a surfactant, a moisturizer, a polyol, a tackifier, and a water-soluble solution. Polymer, film forming agent, water-insoluble polymer, oil gelling agent 'polymer latex, powder, pigment, dye, precipitated colorant, lower alcohol, sugar, ultraviolet absorber, amino acid other than the above, vitamin Classes, whitening agents, skin revitalizing agents, blood circulation promoting agents, anti-lipid leakage agents, anti-inflammatory agents (anti-inflammatory agents), plant extracts, organic acids, organic amines, metal ion blocking agents, pH adjusters, antioxidants , antibacterial agents (antiseptic fungicides), astringents, cooling agents, spices, water, etc. Among the above-mentioned arbitrary components, specific examples of the oil component include, for example, hardened oil, wood wax, cocoa butter, hardened castor oil, olive oil, castor oil, Hawaiian volcanic soybean oil, evening primrose oil, etc.; beeswax, lanolin, Whale-12- 200916118 oil, candelilla wax 'carnauba wax, insect wax, jojoba wax, jojoba oil, liquid lanolin and other waxes; solid paraffin, pure ceresin, microcrystalline sable , hydrocarbon oil of polyethylene suspected wax, mobile chain smoke, triacontane, polybutylene, volatile hydrocarbons, etc.; cetyl palmitate, isopropyl myristate, isopropyl palmitate, 2 -hexadecylethylhexanoate, octyldodecyl myristate, octyl stearate, octyl palmitate, diisopropyl adipate, 2-ethylhexyl sebacate, dicapric acid Neopentyl glycol ester, triterpenic acid glyceride, tris-2-ethylhexanoic acid glyceride, tricaprylin, triisostearic acid glyceryl diisostearic acid diglyceride, triisobutyl Diglyceride fatty acid, pentaerythritol tetraethyl 2-hexanoate, pentaerythritol dioctoate, trimethylolpropane tri-2-ethylhexanoate, Ester oils such as trimethylolpropane stearate, diisostearyl malate, etc.; stearic acid, palmitic acid, meat citrate, 12-hydroxystearic acid, behenic acid, isostearyl Higher fatty acids such as acid and oleic acid; higher alcohols such as cetyl alcohol, stearyl alcohol, sesquiterpene alcohol, behenyl alcohol, isostearyl alcohol, oleyl alcohol, octyldodecanol, etc. a chain polyoxyalkylene such as methyl polyoxyalkylene, methylphenyl polyoxyalkylene or diphenyl polyoxyalkylene, octamethylcyclotetraoxane, decamethylcyclopentaoxane, Modified polyoxyxane such as cyclic polyoxyalkylene, amido modified polyoxyalkylene, alkyl modified polyoxyalkylene, fluorine modified polyoxyalkylene or the like such as dodecamethylcyclohexaoxane Alkane, etc. The oil system may be selected singly or in combination of two or more. Among the above-mentioned optional components, specific examples of the polyhydric alcohol include glycerin, 1,3-butanediol (1,3 - BG), 1,2-pentanediol, erythritol, sorbitol, and wood. Sugar alcohol, maltitol, propylene glycol, dipropylene glycol (DPG), diglycerin, and the like. The polyol system can be arbitrarily selected from one type or two or more types from -13 to 200916118. Among the above-mentioned optional components, examples of the humectant include, for example, lactate (sodium or the like), sodium pyrrolidonecarboxylate (p c A sodium), urea, and the like. The humectant can be arbitrarily selected from one type or two or more types. Among the above-mentioned optional components, specific examples of the water-soluble polymer include guar gum, quince seed, carrageenan, galactan, gum arabic, pectin, and mannan. Starch, Pluron, Sanxian Gum, Curdlan, methylcellulose, hydroxyethylcellulose, carboxymethylcellulose, methylhydroxypropylcellulose, Chondroitin sulfate , dermatan sulfate, hepatic sugar, heparan sulfate, hyaluronic acid, sodium hyaluronate, tragacanth, keratan sulfate, chondroitin, mucoitin sulfate , Ethyl guar gum, carboxymethyl guar gum, dextran, Kerato sulfate, locust bean gum, sue cino glue an, caronine, carapace Quality, chitosan, carboxymethyl chitin, agar, polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, sodium polyacrylate, PEG, and the like. The water-soluble polymer may be optionally used singly or in combination of two or more. The external preparation for skin of the present invention is formulated in accordance with the usual method in combination with the above ingredients. Further, as described above, when the external preparation for skin is prepared, the liposome can be formed simultaneously with the preparation of the external preparation for skin. The form of the external preparation for skin of the present invention is not particularly limited, and examples thereof include an emulsion, a cream, a lotion, a mask, and the like. In addition, the dosage form is not particularly limited, and examples thereof include a gel, a dispersion, an ointment, an external solution, and a cream of -14 to 200916118. In particular, a gel system is preferable in terms of a moisturizing effect. [Embodiment] EXAMPLES Hereinafter, the present invention will be specifically described by way of examples. The blending amount is not specifically described as the mass%. Before explaining the examples, an effect test method for use in the present invention will be described. Moisturizing effect The effect test was carried out by a female investigator who has experience in the evaluation of cosmetic use sensibility. The investigator applied a suitable amount of the test article to the face after washing the face, and evaluated the moisturizing feeling after 2 hours of application. (Evaluation criteria) ◎: Strongly moisturizing 〇: Feel moisturizing △: Moisturizing slightly: X: No moisturizing [Preparation of amino acid solution] The components disclosed in Table 1 were mixed to prepare amino acid solutions A to D. -15- 200916118 Table 1 Amine acid (% by mass) ABCD Tryptophan (iso) 3.1 1.55 0.05 Histidine (iso) 3.1 1.55 0.25 Proline (fat) 0.50 leucine (fat) _ - 0.70 Amino acid (fat) _ - 0.30 sulphate (fat) - 0.30 lysine (fat) latitude - 0.60 methionine (fat) - 0.10 phenylalanine (aryl) - 0.30 1,3-BG 15.0 15.0 15.0 15.0 Remaining amount of the remaining amount of the remaining amount of the purified water [Preparation of the yeast extract liquid] The yeast extract liquid A (as a pure dry component of the yeast extract was 1.5% by mass) formed by the components disclosed in Table 2 was prepared. The modulation system was Cytocatalyzer BG30 (manufactured by Yamakawa Trading Co., Ltd.). Table 2 Yeast Extract Liquid Α 筲, %, Yeast Extract-----JL-Bingli/υ 7 1 . 5 1,3-BG ^00 Refined Water - One __ Remaining Quantity [Modulation Lipids] The components shown in Table 3 were used, and the ratios of the components used in Table 3 were as follows: the total amount of the compound was 100 A to D system 袠1. It is expressed by mass% 33% by mass. In addition, the amino acid solution '16-200916118 mother extract liquid A is shown in Table 2. After mixing the hydrogenated soybean phospholipids, glycerin, and a portion of D P G at 50 ° C, a mixture of the remaining portion of D P G and cholesterol was added and dissolved by heating at 50 °C. Thereafter, yeast extract A and the amino acid solution were added and stirred and mixed. Thereafter, the purified water was added to a mass ratio of 100%, and the mixture was stirred. Finally, it was treated with a high-pressure emulsifier ("micro fluidizer", manufactured by Micro Fluidics, USA) to obtain a liposome. The obtained microlipid has an average particle diameter of 45 nm. Further, Table 3 discloses the components of the non-lipids A and B which are combined with the non-lipidal composition of the yeast extract A and the amino acid A or B. This non-lipid system is prepared by mixing the components. Table 3 Microlipids (% by mass) Non-micro-left (mass body [%] ABCDEFGAB Hydrogenated soybean phospholipids (Note) 2.0 2.0 2.0 2.0 2.0 2.0 2.0 . _ Glycerin 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 DPG 12.0 12.0 12.0 12.0 12.0 12.0 12.0 12.0 12.0 Cholesterol 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Yeast Extract A 20.0 20.0 40.0 _ 20.0 20.0 20.0 20.0 Amino Acid A 5.0 — 10.0 • I. 5.0 Amino Acid B • 5.0 • - 10.0 _ Qin Mi 5.0 Amino acid C _ 5.0 _ Amino acid D _ _ _ _ _ 5.0 Refining water Residual remaining Residual remaining Residual remaining Residual amount Amount amount — 10 (made by Nikko Chemicals Co., Ltd.) -17- 200916118 [Preparation of skin external preparation] [Examples 1 to 4, Comparative Examples 1 to 5] Skin external preparations (gel creams) are added to the amounts specified in Table 4 As a gel preparation. Further, the liposomes A to G and the non-lipid bodies A and B are shown in Table 3. Table 4 Real _ Comparative Example _ 1 2 1 2 3 4 5 3 4 Glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 1,3-BG 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 Carboxyvinyl polymer 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 Acrylic acid alkyl methacrylate copolymer 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Phenoxyethyl hydrazine 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 1,2-adipate 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Potassium hydroxide 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Microlipid A 25.0 _ Microlipid B 25.0 _ - _ Two _ _ Microlipid C _ 25.0 _ _ One - _ • Microlipid D . . 25.0 _ _ _ . _ Microlipid E - - Age 25.0 _ . A _ Microlipid F - . - _ _ _ 25.0 _ Microlipid G - - - _ _ _ . _ 25.0 Non-lipid A • - _ 25.0 _ , non-lipid body B - - _ _ 25.0 _ - refined water remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount About Examples 1 to 4, Comparative Example 1 ~5 skin external preparation (gel-18- 200916118 cream), effect test] The evaluation results are shown in Table 5. . Table 5 Moisturizing sensation (number of people) ◎ 〇Δ X Example 1 6 4 0 0 Example 2 6 4 0 0 Comparative Example 1 0 3 7 0 Comparative Example 2 0 3 7 0 Comparative Example 3 0 3 7 0 Comparative Example 4 0 4 5 1 Comparative Example 5 0 4 5 1 Example 3 8 2 0 0 Example 4 10 0 0 0 It can be seen from Table 5 that a tryptophan and/or histidine containing a yeast extract and a heterocyclic amino acid are blended. A skin external preparation for a liposome, a skin external preparation containing a liposome containing only a yeast extract (Comparative Example 1), and a skin external preparation containing a liposome containing a tryptophan or histidine (Comparative Example 2) 3), and a skin external preparation (Comparative Examples 4 and 5) which is combined with a yeast extract and a non-lipid body of tryptophan or histidine, and which gives the skin an excellent moisturizing feeling. Further, it is known that a heterocyclic amino acid formed from a tryptophan acid and/or a histidine acid as an amino acid is further compounded with lysine, leucine, isoleucine, threonine, lysine. The skin-based external preparation for the aliphatic amino acid formed by methionine and the aromatic amino acid formed by phenylalanine (Example 4) is more moisturizing to the skin. [Examples 5 to 8] -19-200916118 The following are examples of the external preparation for skin according to the composition of Table 6 of the present invention (Examples 5 to 8 (gel cream)). Further, the production was carried out in accordance with the methods of Examples 1 to 4. Table 6 was prepared in the same manner as in Table 3 except that the liposome Η~K system was used, and the one shown in Table 7 was used. Further, the amino acid hydrazines to D in Table 7 are shown in Table 1, and the yeast extract liquid oxime is shown in Table 2. Table 6 Example (% by mass) 5 6 7 8 Glycerol 5.0 5.0 5.0 5.0 1,3 -BG 8.0 8.0 8.0 8.0 Carboxyvinyl polymer 0.6 0.6 0.6 0.6 Acrylic acid alkyl methacrylate copolymer 0.2 0.2 0.2 0.2 benzene Oxyethanol 5000 0.5 0.5 0.5 1,2-hexanediol 0.05 0.05 0.05 0.05 Potassium hydroxide 0.3 0.3 0.3 0.3 Microlipid Η 25.0 - - _ Microlipid I 25.0 . _ Microlipid J _ 25.0 Microlipid Κ - _ 25.0 Residual amount of refined water Residual amount Remaining amount -20- 200916118 Table Ί Microlipid (% by mass) Η IJ 氢化 Hydrogenated soybean phospholipid (Note) 2.0 2.0 2.0 2.0 Glycerin 20.0 20.0 20.0 20.0 DPG 12.0 12.0 12.0 12.0 Cholesterol 0.2 0.2 0.2 0.2 Yeast Extract A 20.0 20.0 20.0 20.0 Amino Acid A 0.00 1 . Amino Acid B 0.1 _ • Amino Acid C __ 0.0001 Amino Acid D . 0.0 1 Remaining Water Remaining Amount Remaining amount of remaining amount (Note) NIKKOL Lecinol S - 10 (made by Twilight Chemicals Co., Ltd.) [Examples 9 to 17] The following are examples of skin external preparations which are not shown in the composition of Table 8 of the present invention ( Example 9 to 1 7 ( Gel cream)). Further, the production was carried out in accordance with the methods of Examples 1 to 4. The microlipids A, B, and L to S in accordance with Table 8 were as shown in Table 3 and the same as those shown in Table 9 prepared in the same manner. Further, the amino acid solutions E to L in Table 9 are shown in Table 10, and the yeast extract liquid A is shown in Table 2. -21 - 200916118 Table 8 Example (% by mass) 9 10 11 12 13 14 15 16 17 Glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 1,3-BG 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 Carboxyvinyl polymer 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 Acrylic acid alkyl methacrylate copolymer 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Phenoxyethanol 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 1,2-hexanediol 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Potassium hydroxide 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Microlipid A 12.5 Microlipid B 12.5 Microlipid L 25.0 _ _ _ _ . _ _ Microlipid Μ - 25.0 _ • _ _ _ • Microlipid Ν . _ 25.0 _ _ . _ _ Microlipid 〇 _ _ . 25.0 _ _ — _ _ Microlipid Ρ - _ _ • 25.0 - • Microlipid Q _ _ • _ 25.0 _ _ _ microlipid R _ - _ • 25.0 micro-lipid S - - one - _ _ _ 25.0 _ refined water remaining residual remaining residual remaining residual remaining amount amount Lipid (% by mass) L Μ Ν 0 Ρ 0 RS hydrogen Soy Phospholipid (Note) 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Glycerin 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 DPG 12.0 12.0 12.0 12.0 12.0 12.0 12.0 12.0 Cholesterol 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Yeast Extract A 2.0 5.0 30.0 10.0 15.0 10.0 15.0 20.0 Amino acid solution E 0.5 Amino acid solution F 0.05 _ _ _ Amino acid solution G 0.003 _ _ . _ Amino acid solution • • • 0.0003 • Amino acid solution I • • . 0.05 _ __ • Amino acid solution J _ • • _ 0.0005 • _ Amino acid solution • • • _ 0.03 Amino acid solution L • Release • 0.005 Refining water remaining residual remaining amount remaining residual remaining amount amount NIKKOL Lecinol S — 10 (made by Nikko Chemicals Co., Ltd.) -23- 200916118 Table oxime acid solution (mass ° /. EFG Η IJ Κ L Tryptophan (iso) 0.05 0.35 0.26 0.14 0.1 0.35 . _ histidine (iso) 0.3 _ 1.34 0.2 0.17 . 0.35 1.3 proline (fat) 2.75 - - 0.4 0.5 _ leucine ( Fat) - Hua _ 0.4 0.5 2.0 _ Isoaliline (fat) _ - - 0.34 0.5 - 2.45 _ sulphate (fat) _ _ _ 0.3 0.4 0.45 _ lysine (fat) 2.35 _ 0.3 0.4 a _ A Thioric acid (fat) - 0.4 _ 0.3 0.4 _ - phenylalanine (aryl) - - 1.5 0.72 0.13 0.3 0.3 1.8 1,3-BG 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 Refining water remaining amount remaining amount remaining amount remaining amount remaining Remaining amount remaining amount remaining amount [Examples 18 to 20] The skin external preparation of the present invention is additionally shown below. Further, the amino acid solution and the yeast extract liquid used were prepared as follows. [Preparation of amino acid] The components disclosed in Table 1 were mixed to prepare guanidine amide, N. -24- 200916118 Table 11 Amino acid (% by mass) Μ Ν Tryptophan (iso) 16.0 1.6 Histamine (iso) 84.0 8.0 Proline (fat) - 16.1 Amino acid (fat) - 22.6 Isoleamine Acid (fat) - 9.7 sulphate (fat) - 9.7 lysine (fat) - 19.4 methionine (fat) - 3.2 phenylalanine (aryl) - 9.7 [Preparation of yeast extract] Mixed by Table 1 2 The disclosed components were prepared as yeast extract liquids B to D having a pure dry component of yeast extract of 〇·4% by mass (B), 2.0% by mass (C), and 4.0% by mass (D). In addition, these yeast extract solutions were prepared using Cytocatalyzer BG30 (manufactured by Yamakawa Trading Co., Ltd.). Table 12 Yeast extract solution (% by mass) Β C D Yeast extract 0.4 2.0 4.0 1 , 3 -BG 8.0 40.0 80.0 Refined water 9 1.6 5 8.0 16.0 Example 1 8 lotion -25- 200916118 Ingredients

(1 ) 1,3- BG(1) 1,3- BG

(2 ) DPG (3 ) 苯氧基乙醇 (4) 精製水 (5 ) 氫化大豆磷脂質(2) DPG (3) phenoxyethanol (4) refined water (5) hydrogenated soybean phospholipid

(6 ) 1 ,3 - BG (7 ) 膽固醇(6) 1 ,3 - BG (7 ) Cholesterol

(8 ) 胺基酸N(8) Amino acid N

(9 ) 酵母萃取物液B (10) 精製水配合量（質量比） 5.0 2.0 0.2 剩餘量 1.0 8.0 0.5 0.1 25.0 25.0 計 100.0 (製法）以5(TC高壓乳化機處理成份（5 )〜（1 0 )之混合溶液，冷卻至3 (TC，調製包含胺基酸及酵母萃取物之微脂體。攪拌溶解成份（1 )〜（4 )，加入上述微脂體，攪拌混合而得化粧水。實施例1 9化粧水 -26- 200916118 成份(9) Yeast extract liquid B (10) Refined water blending amount (mass ratio) 5.0 2.0 0.2 Residual amount 1.0 8.0 0.5 0.1 25.0 25.0 Calculated 100.0 (manufacturing method) 5 (TC high pressure emulsifier processing component (5) ~ (1 Mix the solution of 0), cool to 3 (TC, prepare a liposome containing amino acid and yeast extract. Stir the dissolved components (1) to (4), add the above-mentioned liposome, and mix and mix to obtain a lotion. Example 1 9 lotion-26- 200916118 Ingredients

(1 ) 1,3- BG(1) 1,3- BG

(6 ) 1,3- BG (7 ) 膽固醇 (8 ) 胺基酸Μ(6) 1,3-BG (7) Cholesterol (8) Amino acid amide

(9 ) 酵母萃取物液C (1〇) 精製水配合量（質量比） 5.0 2.0 0.2 剩餘量 1.0 8.0 0.5 10.0 25.0 24.0 合計 10 0.0 (製法）以5 0 °C高壓乳化機處理成份（5 )〜（1 0 )之混合溶液，冷卻至3 (TC，調製包含胺基酸及酵母萃取物之微脂體。攪拌溶解成份（1 )〜（4 )，加入上述微脂體，攪拌混合而得化粧水。實施例2 0乳霜 -27- 200916118 成份配合量（質量比） (1 ) 流動鏈烷烴 5.0 (2 ) 硬脂酸2-乙基己酯 15.0 (3)凡士林 3.0 (4 ) 十六烷醇 1.0 (5) 聚環氧乙烷（20 )山梨糖醇單硬脂酸酯 1.0 (6 ) 聚環氧乙烷（2 0 )油醚 2.5 (7 ) DPG 5.0 (8 ) 苯氧基乙醇 0.2 (9 ) 精製水剩餘量 (10 ) 氫化大豆磷脂質 1.0 (11) 1 ,3 - BG 8.0 (12 ) 膽固醇 0.5 (13 ) 胺基酸N 10.0 (14 ) 酵母萃取物液D 25.0 (1 5 ) 精製水 1 5 _ 0 合計 10 0.0 (製法）以5 ot高壓乳化機處理成份（1 0 )〜（1 5 )之混合溶液，冷卻至3 0°C，調製包含胺基酸及酵母萃取物之微脂體。以8 (TC加熱混合溶解成份（1 )〜（9 )、乳化，冷卻至 3 Ot，添加上述微脂體，攪拌混合而得乳霜。 -28- 200916118 [實施例21〜22] 以下係表示本發明之皮膚外用劑。(9) Yeast extract liquid C (1〇) Refined water blending amount (mass ratio) 5.0 2.0 0.2 Remaining amount 1.0 8.0 0.5 10.0 25.0 24.0 Total 10 0.0 (Preparation method) Treatment of components with 5 0 °C high pressure emulsifier (5) Mix the solution of ~(1 0), cool to 3 (TC, prepare the liposome containing the amino acid and the yeast extract. Stir the dissolved components (1) to (4), add the above-mentioned liposome, stir and mix Example 2 0 Cream -27- 200916118 Composition (mass ratio) (1) Flowing paraffin 5.0 (2) 2-ethylhexyl stearate 15.0 (3) Vaseline 3.0 (4) Sixteen Alkanol 1.0 (5) Polyethylene oxide (20) Sorbitol monostearate 1.0 (6) Polyethylene oxide (20) Other 2.5 (7) DPG 5.0 (8) Phenoxyethanol 0.2 (9) Remaining amount of refined water (10) Hydrogenated soybean phospholipid 1.0 (11) 1 , 3 - BG 8.0 (12 ) Cholesterol 0.5 (13 ) Amino acid N 10.0 (14 ) Yeast extract D 25.0 (1 5 ) Refined water 1 5 _ 0 Total 10 0.0 (Preparation method) The mixed solution of the components (1 0 ) to (1 5 ) is treated with a 5 ot high-pressure emulsifier, cooled to 30 ° C, and the amino acid and yeast extract are prepared. The micro-lipid of the substance is mixed with 8 (TC) to dissolve the components (1) to (9), emulsified, cooled to 3 Ot, and the above-mentioned liposome is added, and the mixture is stirred and mixed to obtain a cream. -28- 200916118 [Example 21 ～22] The following is a skin external preparation of the present invention.

實施例2 1凝膠乳霜成份微脂體（注1 ) 胺基酸Μ 酵母萃取物液C 甘油 1,3- BG 配合量（質量比） 23.6 1 .0 25.0 7.0 5.0 DPG 苯氧基乙醇羧乙烯基聚合物甲基聚矽氧烷 0.5 0.8 3.0Example 2 1 gel cream component liposome (Note 1) Amino citrate yeast extract liquid C glycerol 1,3- BG compounding amount (mass ratio) 23.6 1 .0 25.0 7.0 5.0 DPG phenoxyethanol carboxylate Vinyl polymer methyl polyoxane 0.5 0.8 3.0

0.1 四2-己基癸酸抗壞血酸 0.2 尿囊素 0.1 氫氧化鉀 0.3 精製水剩餘合計1 〇〇 . 〇 (注1 )混合下表1 3所成成份，以5 0〜6 0 °c高速攪拌處理，冷卻至3 (TC所調製之微脂體。 -29- 200916118 表 13 配合量（質量％ ) 氫化大豆磷脂質（注2 ) 0.8 甘油 20.0 DPG 12.0 膽固醇 0.2 精製水剩餘量 (注 2) NIKKOL Lecinol S — 10(日光 Chemicals 公司製） [製法] 於微脂體（注1 )混合胺基酸]VI及酵母萃取物液c，以50〜60°c高速攪拌’使微脂體（注1)內包上述胺基酸及上述酵母萃取物。另一方面，加入羧乙烯基聚合物，使分散於精製水’加入氫氧化绅中和，使形成凝膠。接著，加入剩餘的成份於此凝膠’再添加上述包含胺基酸及酵母萃取物之微脂體攪拌，調製凝膠乳霜。實施例2 2凝膠乳霜 -30- 200916118 微脂胺基酵母 POE 甘油 1，3 - 苯氧羧乙四2 - 氫氧尿囊精製 (注公司成份體（注1 )0.1 Tetra-hexyl decanoic acid ascorbic acid 0.2 Allantoin 0.1 Potassium hydroxide 0.3 Refining water remaining total 1 〇〇. 〇 (Note 1) Mix the ingredients in Table 1 below and mix them at 5 0~60 °C , cooled to 3 (TC-modulated liposome. -29- 200916118 Table 13 Compounding amount (% by mass) Hydrogenated soybean phospholipid (Note 2) 0.8 Glycerin 20.0 DPG 12.0 Cholesterol 0.2 Residual water content (Note 2) NIKKOL Lecinol S - 10 (manufactured by Nikko Chemicals Co., Ltd.) [Production method] Mixing amino acid] VI and yeast extract liquid c in a liposome (Note 1), stirring at a high speed of 50 to 60 ° C to make a liposome (Note 1) The above-mentioned amino acid and the above yeast extract are contained. On the other hand, a carboxyvinyl polymer is added, and the dispersed in purified water is added to the cerium hydroxide to form a gel. Then, the remaining components are added to the gel. The gelatin is further added with the above-mentioned liposome containing amino acid and yeast extract to prepare a gel cream. Example 2 2 Gel Cream -30- 200916118 Microlipid Amino Acid POE Glycerin 1,3 - phenoxy Carboxyate B 2 - Hydrogen Peroxide Purification (Note company composition body (Note 1 )

酸N 萃取物B (60 )硬化箆麻油Acid N Extract B (60) Hardened Castor Oil

BG 基乙醇稀基聚合物 -己基癸酸抗壞血酸化鉀素水 1 ) NIKKOL Aquasome 製）配合量（質量比） 27.1 0.1 25.0 0.1 3.0 7.0 0.5 0.7 0.2 0.3 0.1 剩餘合計 100.0 BH CONC (日光 Chemicals 表 1 4 成份聚環 1,3 - 氫化 1,2 — 精製 1 . 0質量％ 6.0質量％ 4.0質量。/〇 4.5質量％ 8 4 · 5質量％BG-based ethanol-based polymer-hexyl phthalic acid ascorbate potassium water 1) NIKKOL Aquasome) compounding amount (mass ratio) 27.1 0.1 25.0 0.1 3.0 7.0 0.5 0.7 0.2 0.3 0.1 Remaining total 100.0 BH CONC (Daylight Chemicals Table 1 4 Ingredient Polycyclic 1,3 - Hydrogenated 1,2 - Refined 1.0% by mass 6.0% by mass 4.0 by mass. /〇4.5% by mass 8 4 · 5 mass%

氧乙烷硬化箆麻油（60EO ) BG 大豆磷脂質戊二醇水 -31 200916118 [製法] 與實施例2 1同樣地調製凝膠乳霜。產業上利用性配合包含酵母萃取物及特定的胺基酸之微脂體之皮膚外用劑’給予肌膚相乘的保濕效果，例如應用於包含醫藥部外用品之化粧品、醫藥品等各種範圍。 -32-Oxygenethane hardened castor oil (60EO) BG Soy lecithin pentanediol Water - 31 200916118 [Production method] A gel cream was prepared in the same manner as in Example 2 1. Industrial Applicability The skin external application agent containing a yeast extract and a specific amino acid-based liposome is used to impart a moisturizing effect to the skin, and is used, for example, in various fields including cosmetics and pharmaceuticals containing pharmaceutical products. -32-

Claims

200916118 X. Patent application scope 1 1. A skin external preparation comprising the components (A) and (B): (A) yeast extract, (B) one or two selected from the group consisting of tryptophan and tissue A liposome of a group of amino acids (Liposome). 2. The external preparation for skin according to item 1 of the patent application, wherein one or more of the components (B) are further selected from the group consisting of lysine, leucine, isoleucine, threonine, lysine Amino acids in the group of acids, methionine and phenylalanine. -33- 200916118 七无明说单单单符表 is the map of the generation of the map table on behalf of the map: the representative of the map this table ' ' generation \ ly set one or two without eight, if the case has a chemical formula, please reveal the most Chemical formula that can show the characteristics of the invention: none