TW200813324A - Fluidic device and controlling method thereof - Google Patents

Abstract

A fluidic device for performing assays can include control components such as vacuum pumps, gas pumps, "broken open valves," and "self-close valves" for controlling the flow of fluids in the fluidic device. The vacuum pump can be used to pull a fluid in a specific direction in a channel, and the gas pump can be used to push a fluid in a specific direction in a channel. The broken open valve can be used to connect two separate regions at the control of a user, and the self-close valve can be used to automatically seal off a channel after passage of a fluid. The vacuum pumps, gas pumps, broken open valves, and self close valves can be made small in volume so that the fluidic device can be made as a small and portable device.

Description

200813324 F6495001OTWC1 22169-1-twf.doc/006 IX. Description of the Invention: [Technical Field of the Invention] This description relates to a fluid device and a control method therefor. [Prior Art]

Many types of test devices can be used to detect the presence of a compound or to analyze a biochemical reaction. For example, a lateral flow membrane having - or a plurality of test lines along the length direction can be used to perform lateral:, Lateral Flow Assays. The fluid with the dissolved reagent travels from one end of the membrane to the test line by electroosmosis. The reader detects if a reaction has occurred at the test line indicating the presence or absence of certain particles in the reagent. As another example, a garment having a set of microcapillaries can be used to control fluid flow in an immunoassay process. The reagent is positioned at a plurality of locations along the length of the microcapillary such that when the fluid flows in the microcapillary due to capillary forces, the fluid contacts the reagent. The reader monitors the location at which the test is located to determine if a reaction has occurred. As a further example, by controlling the flow of fluid through a plurality of channels and chambers, the microfluidic wafer can be used to perform the assay. The microfluidic wafer can be used with an external power source and/or pump that provides a driving force to drive the fluid. SUMMARY OF THE INVENTION The present invention provides a fluid device for performing an assay, including, for example, ', a work pump, a gas pump, a Broken open valve, and a self-close valve, a control assembly for controlling fluid flow in the fluid device. In the fluid device for performing the assay proposed by the present invention, 5 200813324 P6495001OTW Cl 22169-1-twf.doc/006 can be used and a gas pump vacuum pump can be used. Traction fluid in the channel in a particular direction to push the fluid in the channel in a particular direction. Age =, the proposed fluid device for performing the assay, can use the = open valve to connect two separate areas controlled by the user, A self-closing valve can be used to automatically seal the passage after the fluid passes. = Ming: The fluid device proposed for the execution of the test, the vacuum " ι mess: the open type wide and the self-closing valve can be made into a volume ^Reading, so that the fluid device can be made into a very small and portable tunnel 2: for a difference, so that the fluid moves in the passage and generates a gas outside the pressure vessel in the overnight The gas pressure of the body pressure, or (8) package::: from the material ΐ first - the container is disconnected before - the second material and the second material are mutually: two in order to make the first - in the first channel and the - container. Pressure difference. A Lai, the first - Rong riding channel produced the gas pressure of the gas pressure defining the (fourth) - container (1), or including 3 = = outside the container = = before the device is disconnected and - the second material phase " = brother - the material is selected such that the first material and the second material interact with each other immediately after the generation of the gas twfdoc/006. The present invention provides a fluidic device comprising a first material defining a first region to define a second material of the second region, the second region being separated from the first region. The connector further includes a connector coupled between the first region and the second region, the connector comprising a brittle material and having an open end and a closed end. This opening, the end is disposed in the second region, and the closed end is disposed in the first region. The first region is separated from the second region by the closed end of the connector. The connection is set such that when the closed end of the connector is broken, the connector defines a passage from the first region to the second region. The present invention provides a fluid device comprising a channel of an expanded portion having a diameter greater than an adjacent portion of the channel and further comprising a material disposed in the expanded portion. This material has a volume that does not block the passage of the fluid prior to adsorption of the fluid. Where the material adsorbs a portion of the fluid, the volume immediately expands such that the expanded material blocks the passage of additional fluid through the passage. This material contains a super adsorbent polymer. The present invention provides a fluid device comprising a first 10 reservoir containing a first fluid, a second reservoir containing a second fluid, a main channel, a first branch channel, a second branch channel, and a first Single use pump and second single use - pump. This first branch channel is coupled to the first reservoir to the main channel. The second-branch channel is coupled to the second reservoir to the main channel. The first single use pump then creates a pressure differential to move one or both of the first fluid and the second fluid when the container in the first single use pump is disconnected. And when the container in the second single use pump is disconnected, the second single use pump generates a pressure difference to move one of the first fluid and the second fluid or both. 7 200813324 P64950010TWC1 22169-l-twf.doc/ 〇〇6. The present invention provides a method of controlling a fluid comprising: lifting to enable sampling of a predetermined amount of fluid. Each of the straws includes a suction: ^Container' This container creates a pressure differential in the passage when it is disconnected, and this is brittle and made of material. (4) in the container boundary (4), the space and the gas pressure of the gas pressure of the ' part is interrupted; the pass = the predetermined amount of pressure difference, so that the predetermined amount of fluid "the method of controlling the fluid from the shishi, including The fluid can be moved from the range of the range to the second area, wherein the first area is lightly connected to the second area by the connector having the closed end, and the open end is in the middle area, and the The closed end is disposed in the first region. The first end of the connector is separated from the second region, wherein the flow packet is disconnected from the closed end of the connector to form a secret through the connector region to the second region. The portion of the fluid flowing through the connector is adsorbed by the use of the material after the volume of the body, and the expanded material is used to block further fluid flow further through the connector. The present invention provides a method of controlling a fluid, including Fluid in the channel: moving, and the channel includes a button that adsorbs the portion of the fluid immediately after the volume expands, causing the fluid to flow in the channel includes flowing a first portion of the fluid through the material and using the material to adsorb the fluid The second part, thereby causing the material, & expansion, to block the passage of additional fluid through the passage by using the expanded material. The present invention provides a method of controlling a fluid comprising passing a fluid through a pass 8 200813324 P6495001OTWC1 22169- 1-twf.doc/006 Road 'This passage includes a first self-closing valve and a second self-closing valve. The first self-closing valve and the second self-closing valve are spaced apart from each other, and each self-closing valve includes a portion that adsorbs fluid a fluid adsorbing material that expands immediately after the volume. The portion of the fluid is adsorbed by using the first self-closing valve and the fluid adsorbing material in the second self-closing valve. The expanding fluid adsorbs the volume of the material to block the passage of the additional fluid further through the passage. Maintaining a predetermined amount of fluid in a portion between the first self-closing valve of the passage and the second self-closing valve.

The present invention provides a method of controlling a fluid comprising blocking a first container made of a brittle material to create a pressure differential in the passage such that the first fluid moves from the first reservoir to the first section of the passage. The first container (a) defines a space within the first container, the space having a gas pressure different from the pressure of the gas outside the first container, or (b) including a first material, the first material, the first container being disconnected The front material is separated from the second material, and the first material and the second material are selected such that a gas is generated immediately after the first material interacts with the second material. The second volume made of a brittle material is blocked to create a pressure difference such that at least a portion of the first fluid is transferred to the second section. Oral Benjamin provides a method of controlling fluid, including simultaneously operating a pump and a second single use pump to draw a first P knife of the fluid to the second pass and a second sample of the sample fluid. Blocking the first pass of the first single use pump makes the first part of the sample fluid self-generated, and the second part of the fluid moves from the reservoir to break the second single use pump (four) through f and resistance - -^ - ' Produces a difference in force, making the sample the second channel. Simultaneous operation 9 200813324 P64950010TWC1 22169-l-twf.doc/〇〇6 Single use pump and fourth single use pump to draw the first buffer solution to the first channel and draw the second buffer solution to the second channel. ^ [Embodiment] The fluid device for performing the verification may include, for example, a vacuum pump, a blown open valve of a gas pump, and a self-closing valve (self-d〇se valvesy, a control component, It is used to control fluid flow in a fluidic device. A vacuum pump can be used to pull the 0 gripper in the channel in a particular direction and a gas pump can be used to push the fluid in the channel in a particular direction. The user controls two separate zones and can use a self-closing valve to automatically seal the passage after the fluid has passed. The vacuum pump, gas pump, disconnect open valve, and self-closing valve can be made small so that the fluid device can be made small And in the following description, individual control components can be introduced first, and then a description of how the control group 2 can be combined to construct a modularized unit for controlling fluid in the fluidic device. How to use it will be described later Fluid bioassay. τ Lu can be constructed by placing the container 1 in the channel 1〇6 (or chamber) defined by the material 1〇2. The vacuum pump 9 〇. The container 1 〇〇 closed area 104 'area 1 〇 4 is vacuum or has a lower gas force than the gas pressure in the channel 1 〇 6. Referring to Figure 1 容器, the container 100 can be, for example, glass The capillary, 1 is disconnected immediately after the external force is applied. When the container 1 is disconnected, the gas in the channel ι 6 / flows into the vacuum region 104 'to reduce the pressure in the region just before. 1〇8 draws the fluid so that it faces the attraction of the region just 200813324 P6495001OTWC1 22169-1-twf.doc/006. Figures 25A-25C show an example of a vacuum pump using a glass capillary placed in a rubber hose. Figure 25A shows In cross-section of gas pump 410, gas pump 410 has a vacuum glass capillary 416 disposed in rubber tube 418, wherein tube 418 has a closed end 424 and an open end 426. Figure 25B shows a cross section of gas pump 412, similar to gas pump 412 The gas pump 410 has, in addition to having a rubber tube 420 with two open ends. Figure 25C shows a gas pump 412 coupled to two rubber tubes 428, wherein the rubber tube 420 has an inner diameter that is larger than the rubber tube 428 ( A glass capillary 416 is housed. Figures 26A and 26B show an example of a vacuum pump using a glass capillary placed in a planar fluid channel. Figure 26A shows a cross section of a vacuum pump 430 having a fluid placed on a planar substrate 434 The vacuum glass capillary 416 in the channel 438. The fluid channel 438 has a closed end 440 and an open end 442. The planar substrate 434 is made of a rigid material. The elastic layer 436 is post-into the substrate 434 adjacent the capillary 416, allowing the user An external force is applied via the elastic layer to break the capillary 416. Figure 26B shows a cross section of a vacuum pump 432 that is similar to vacuum pump 430 except that fluid passage 438 is coupled to two fluid passages 444 having a smaller cross section. A vacuum glass capillary can be made by heating one end of the glass capillary to melt the glass to form a first closed end. A vacuum pump is used to draw air out of the glass capillary via the open end. The glass capillary is heated at a distance from the first closed end. The heat softens the glass and can pinch or twist the softened glass to form a second closed end. 11 200813324 P6495001OTWC1 22169-1-twf.doc/006 Referring to Figure 2A, gas pump 92 can be constructed by placing container no in channel 106 (or chamber) defined by material 1〇2. The container 11 encloses the $' region 112, which has a higher gas pressure than the gas pressure in the channel 1〇6 outside the container 11〇. Referring to Fig. 2B, the container 110 can be, for example, a glass capillary that is broken immediately after an external force is applied. When the container 110 is disconnected, the gas originally inside the container flows out of the container 110, thereby increasing the pressure in the area 1〇6. • In this way a force is generated that can push the fluid in direction I14 away from region 106. In this description, the term "vacuum pump" will be used to refer to a device for generating a pull that can be used to pull fluid toward the device; and a gas pump will be used to refer to a device that generates a push force that can be used to push The fluid is moved away from the device. There is an alternative way to construct the gas pump. For example, referring to FIG. 3A, the glass capillary 12 partially filled with the first material 126 can be placed in the second material 128. A gas pump 94 is fabricated in the channel 124 (or chamber). The first material 126 and the second material 128 are selected such that when the material 126 and the material 128 are mixed with each other, the two will interact and produce one or the other species, body = For example, the first material 126 can be sodium carbonate (Na2C〇3) and/or sodium bicarbonate (NaHC〇3), and the second material 128 can be acetic acid (CH2COOH). Referring to Figure 3B, when an external force is applied to break When the glass capillary 120 is opened, the first material 26 interacts with the first material mg to generate a gas. In this example, the gas is carbon dioxide (C〇2). The chemical reaction that occurs is ·· 12 200813324 P64950010TWC1 22169-1 -twf .doc/006

Na2C03 + 2 CH2COOH + 2 NaCOOCH2 + H20 + C02

NaHC03 + CH2COOH NaCOOCH2 + H20 + C02 > Monooxide increases the pressure in channel 124, creating a force that can be used to push the fluid away from the broken capillary 120. The first material 126 can be directly filled into the capillary 12〇. Referring to Fig. 27A, the first material 126 can also be attached to the wire 450, and the wire can then be placed inside the capillary 12〇 with the wei wei material 126. Figure 27B shows an example of placing a glass capillary 120 in a channel 124 in a rubber tube 418. Channel 124 contains a second material 128 that can interact with first material 126 when glass capillary 120 is broken. Figure 27C shows an example of placing glass capillary 120 in fluid channel 438 within planar device substrate 434. The elastic layer 436 is embedded in the substrate 434 adjacent to the capillary 120 such that the user is allowed to apply an external force via the elastic layer 436 to break the capillary 120. Referring to FIG. 4A, a gas pump 96 can be fabricated by placing the compound 130 in a glass capillary 132, sealing the capillary 132, heating the capillary 132, cooling the capillary 132, and placing the capillary 132 in the overnight 106 (or In the chamber). Compound 13 () was selected as a material which generates a gas after heating. When the capillary 132 is heated and cooled, the gas generated by the compound 13A increases the gas pressure inside the capillary 132 (compared to the gas pressure outside the capillary 132). Examples of the compound 130 include sodium hydrogencarbonate (NaHC〇3) and calcium carbonate (CaC〇3). These compounds produce carbon dioxide when heated:

NaHC03 NaOH + C〇2 13 200813324 P64950010TWC1 22169 small twf.doc/006

CaC03 CaO + C02 can also be used as a sublimation material that changes from a solid state to a gaseous state (for example, it becomes a dry ice of (^^). The material table of Fig. 4B lists other materials which generate gas when heated or nitrogen gas generated by heating like NaN3 ( 2 NaN3 -> 2Na + 3N2) 〇 Referring to Fig. 5A, a break open valve i4〇 can be fabricated by placing a glass capillary 142 between the first passage 148 and the second passage 150. The glass capillary 142 has positioning The open end 144 in the first passage 148 and the closed end 146 positioned in the second passage 150. When the glass capillary is intact, the fluid cannot flow between the first passage 148 and the second passage 15〇. For the open type, the "closed, state" is shown. Referring to Fig. 5B, when an external force is applied to break the glass capillary 142, the connecting passage 148 is formed with the passage 152 of the passage 150. This is called a "disconnected opening valve". Open "state. Disconnected open valve 14" can be used to allow initial separation of the two fluids (or a fluid and a solid) and then interact at a time controlled by the user. Figures 28A and 28B show the use of breakaway An example of a low cost device is opened by opening a valve for performing a test in which a fluid is irradiated with ultraviolet (uv) light. A glass capillary 142 connects the two plastic channels 46 and 462. Initially, the reactant 464 is included in the first In the plastic channel 462. After the glass capillary 142 is broken, the reactant 464 immediately flows through the glass capillary 142 to the second plastic channel 46. As shown in Figure 28]B, when the reactant 464 flows through the glass capillary 142, the UV light source 466 Radiation Reagent 464. Detector 468 detects UV light passing through Reagent 464. The spectrum of UV light detected by detector 468 can be used to determine the compound in Reagent 464. Figure 28C shows a square inner perimeter And a cross section of the outer periphery of the glass capillary 14 200813324 P6495001OTWC1 22169-1-twf.doc/006. The inner circumference of the square and the outer periphery allow UV light to pass through the glass capillary in a direction perpendicular to the surface of the glass capillary. Compared to the cross-section, the capillary has a square cross-section which allows more UV light to reach the fluid in the glass capillary, where a circular cross-section may 'cause The UV light is reflected or redirected away from the fluid. Referring to Figures 6A and 6B, a self-closing valve can be fabricated by placing a Superabsorbent Polymer ("sap") 162 in the channel 164. 160. Initially, the SAP 162 has a smaller volume and allows fluid to flow between the first zone ι 66 and the second zone 168 in the channel 164 (Fig. 6A). This is referred to as the "open" state of the self-closing valve. As the fluid flows through the SAP 162, the SAP adsorbs a portion of the fluid and expands to block the passage 164 (Fig. 6B), thereby preventing further flow of fluid between the first region 166 and the second region 168. This is referred to as the "off" state of the self-closing valve. Supersorbent polymers adsorb and retain the bulk of water or other aqueous solutions. In certain instances, SAP can be made from chemically modified starches as well as cellulose 10 and other polymers, such as poly(vinyl alcohol) PVA, poly (oxidized) which is highly hydrophilic and has a high affinity for water. Ethylene) pE〇/. 'In some examples, supersorbent polymers can be made from partially neutralized and lightly crosslinked acrylic acid, partially neutralized and lightly crosslinked poly(acrylic acid) with good efficiency 6 relative cost The ratio. The polymer can be made to have a low solids content, and after the Pi, the polymer is dried and ground to a white granular solid. In water, the white solid swells to a rubber gel which in some cases may comprise up to 99% by weight of the water. ^ 15 200813324 P6495001OTWC1 22169-1-twf.doc/006 Referring to Figure 7A, the self-closing valve 170 can include a channel 164 having a flared portion 172 to accommodate the super-adsorbent polymer 162 such that the super-adsorbent polymer 162 The flow of fluid is not limited prior to expansion. To make the self-closing valve no, an adhesive can be applied to the inner wall of the enlarged portion 172, and the SAP 162 in powder form is then pushed into the passage 164, and the SAP 162 powder is adhered to the inner wall at the enlarged portion 172. Referring to Figure 7B, as the fluid flows through the supersorbent polymer 162, the super-φ adsorbent polymer 162 adsorbs a portion of the fluid and expands in volume, thereby breaking the passage 164, thereby preventing further flow of fluid through the expanded polymer 162. . Referring to Figures 8A and 8B, supersorbent polymer 162 can be attached to lead 180 and subsequently placed in channel 164. Channel 164 can have a recessed region 182 that is coated with an adhesive to secure wire 18〇 at a predefined location. Referring to Figure 8C, as fluid flows through the supersorbent polymer 162, the polymer 162 adsorbs a portion of the fluid and expands in volume, thereby blocking the passage 164, thereby preventing further flow of fluid through the expanded polymer 162. • Self-closing valves can be fabricated by coating the wires with SAP and then placing the coated wires in a channel or tube. Self-closing valves for planar fluidic devices can be fabricated by coating a planar substrate with SAP and subsequently placing the coated substrate in a planar communication in a planar fluidic device. Referring to Figures 9A through 9C, the valve 190 can be closed by using a glass capillary 142 and a SApi 62 located outside of the capillary 142 and adjacent to the capillary 142. Capillary 142 and SAP 162 are both positioned in channel 164 having a first region 166 and a second region 168. Use of glass wool 16 200813324 P64950010TWC1 22169-l-twf.doc/006 The thin tube 142 and SAP are similar to the combination of a disconnected open valve and a self-closing valve. Switching the open valve 190 allows the user to control the flow of fluid through a particular location in the channel by allowing, subsequently blocking, and then allowing fluid to pass through a particular location. Referring to Figure 9A, initially, SAP 162 has a smaller volume and does not block the passage, allowing fluid to flow between first region 166 and second region 168. Referring to Figure 9B, as the fluid passes, a portion of the fluid is adsorbed by SAp 162, thereby increasing the volume of SAP 162, thereby blocking further flow of fluid between first region 166 and second region 168. Referring to Figure 9C, when an external force is applied to break the glass capillary 142, a passage 152 is created to allow fluid to flow between the first region 166 and the second region 168. Referring to Figures 10A through 10c, the switch width 200 can be fabricated by using a glass capillary 142 and an SAP 162 positioned inside the capillary 142. Capillary 142 has an open end 144 and a closed end 146. Open End • I44 is positioned in the first channel 148 and the closed end 146 is positioned in the second channel 150. The glass capillary 142 and the SAp 162 perform a function similar to the combination of the open-type open valve and the self-closing valve. Turning off the switching valve 2〇〇 enables the user to control the flow of fluid through a particular location in the channel by blocking, subsequently allowing, and then blocking fluid flow through a particular location. Referring to Figure 10A, when the glass capillary 142 is intact, the first track 148 is not connected to the second channel 150. Referring to Fig. 10B', when an external force is applied to the glass capillary 142, 17 200813324 P6495001OTWC1 22169-1-twf.doc/006 forms a passage 152 to allow fluid to flow between the passages 148 and 15〇. The SAP 162 initially has a small volume and does not block the flow of fluid in the passage 152. Referring to Figure 10C', as fluid flows through passage 152, portions of the fluid are adsorbed by SAP 162, causing the SAP volume to increase and block passage 152, thereby preventing fluid from flowing further through passage 152. Referring to Figures 11A through 11D, the switch-on/off valve can be manufactured by using a glass capillary 142, an SAP 212 positioned inside the capillary I42, and a SAP 214 positioned outside the capillary 142 (〇n_〇ff_〇n_〇 Ff valve). Glass capillary 142, SAP 212, and SAP 214 are placed in channel 164. The glass capillary 142, SAP 212, and SAp2M perform a function similar to the combination of a disconnected open valve and two self-closing valves. Switching on and off the switching valve 210 allows the user to control the flow of fluid through a particular location in the channel by allowing, subsequently blocking, subsequently allowing, and subsequently blocking fluid flow through a particular location. Referring to Figure 11A, initially, the SAP 214 has a relatively small volume and allows flow to flow between the first region 166 and the second region 168 of the passage 164. Referring to Figure 11B, as the fluid passes, a portion of the fluid is adsorbed by SAp 214, thereby increasing the volume of SAP 214, thereby blocking further flow of fluid between the first, region 166 and second region 168. Referring to Fig. lie, when an external force is applied to break the glass capillary 142, the passage 152 is opened to allow fluid to flow between the first region 166 and the second region 168. Referring to Figure 11D, as the fluid flows through the SAP 212, the portion of the fluid is adsorbed by 18 200813324 P64950010TWC1 22169-l-twf.doc/006 = T: thereby increasing the volume of the SAp 212 and blocking the passage 152, which prevents the fluid from furthering Flow through the passage. Referring to Figure 12', a metering straw 22 can be constructed for drawing a predetermined amount of fluid by using the true * = 2 connected to the pipette body 224. The vacuum pump 222 includes a true capillary fry placed in the suction ball. In order to use the metering, the glass capillary S 100 is used to create an attractive force for drawing fluid into the straw body 224.

When the manufacturing-batch metering pipette is 22 inches, the size of the sphere 226 and the glass capillary can be made the same. The ball 226 and the glass capillary tube (10) are used to measure the amount of deformation of the application sphere 226 required to break the glass capillary f1〇〇 when the user presses the ball 226 to break the glass capillary 1〇〇 for all metering straws. 220 is roughly the same. In this manner, the user can quickly draw a predetermined amount of fluid using the metering straw 22G to monitor the fluid content of the stem without f. For example, referring to Figures 21A and 21B, a metering pipette 220 can be used to quickly sample a predetermined amount of blood a from a patient. . Referring to Figure 13, another example of a metering straw 230 includes a vacuum pump 222, and a gas pump 232. The vacuum pump 222 is similar to the vacuum pump shown in FIG. The gas pump 232 includes a glass capillary 12〇, the glass capillary 12〇' is filled with %α)3 and placed in a straw sphere containing CH2C〇〇H. When the glass capillary 120 is disconnected, NhCOs and CH2C〇〇H are mutually Effect: Generate c〇2 to increase the gas pressure in the sphere 234. The vacuum pump 222 allows the user to quickly draw a predetermined amount of fluid into the straw 23. Gas pump 232 allows the user to dispense fluid out of straw 230. 19 200813324 P64950010T WC1 22169-1 -twf.doc/006 The advantage of using gas pump 232 is that when a reaction between Na2C〇3 and CH2COOH produces C〇2 gas, tube 228 can be dispensed during a controlled period of time. Fluid in the middle. In this way, the user does not have to carefully monitor the flow of fluid as it is being dispensed. Referring to Figure 14A, another example of a metering straw 240 includes a ball 242, a middle portion 244, and a straw body 246. The intermediate portion 244 is constructed of a deformable material. Switch open valve 248 is positioned in intermediate portion 244. The switch open valve 248 includes a glass capillary 142 and an SAP 162 positioned outside of the capillary 142, similar to the device shown in Figures 9A-9C. Referring to Figure 14A, in order to use the suction tube 240, the user squeezes and releases the ball 242 to draw fluid into the body 246 and the intermediate portion 244. Referring to Figure 14B, when the fluid reaches the intermediate portion 244 and begins to contact the SAP 248, a portion of the fluid is adsorbed by the sAp 248, causing the sap 248 to expand in volume and block the fluid passage on the other side of the sAP 248. In this manner, a predetermined amount of fluid is drawn into the suction tube 240. Referring to Figure 14C, in order to dispense fluid from the suction tube 240, the user presses the intermediate portion 244 (made of a deformable material) to break the glass capillary 142' to form The passage through the broken capillary 142. The user then squashed the ball 242 to force fluid out of the straw 240. When a plurality of suction tubes 240 are manufactured, the tubular body 240 and the intermediate portion 244 are the same size, and the position of the switch opening valve 248 in the intermediate portion 244 is the same, so that the user can use the suction tube 240 to be precise. In the case of the liquid content in the control tube 240, the fluid is substantially aspirated.里气20 200813324 P6495001OTWC1 22169-1-twf.doc/006 Referring to Figure 15A, a metering device 26 for collecting a predetermined amount of fluid includes a glass capillary 262 having two branches 266a and 266b, two from Valves 268a and 268b are closed, as well as two open-ended valves 270a and 270b. Each of the self-closing valves 268a and 268b has an SAP that expands immediately after adsorbing the fluid. Initially, the self-closing valves 268a and 268b are in an open state, and the disconnected open valves 27A and 270b are in a closed state. Self-closing valves 268a and 268b can be similar to the self-closing valves shown in Figures φ 6A through 8C. The break open valve 27A and 27% can be similar to the open open valve shown in Figures 5A and 5B. In operation, fluid 274 is drawn into capillary tube 262 due to capillary forces and flows through self-closing valves 268a and 268b. Referring to Figure 15B, as fluid 274 flows through self-closing valves 268a and 268b, portions of fluid 274 are adsorbed by SAP in shut-off valves 268a and 268b, thereby changing self-closing valves 268a and 268b to a closed state thereby blocking The progress of fluid 274 is moving. In this manner, fluid 274 occupies a section 264 between the self-closing valves 268a and 268b of the capillary. • The fluid 274 can be moved from section 264 to other locations via branches 266a or 266b by changing the disconnected open valves 27A and 270b from the closed state to the open state and applying an attractive or urging force to move the fluid 274. . • The advantage of the metering device 260 is that the metering device 260 can quickly sample a predetermined volume of fluid without careful monitoring by the user. Because the capillary has a small diameter', the metering device 260 can be used to accurately sample the body. Referring to Figure 16A, three different amounts of fluid can be taken from the sample cell 282. 21 200813324 P6495001 〇TWC 1 22169 small twf.doc/006, the metering device 280 includes three capillaries 284a, 28A and 28A. Each capillary has a self-closing valve (e.g., 286a, 286b, or 286c) at one end and a vacuum valve (e.g., 288a, 288b, or 288c) at the other end. Each vacuum pump has a vacuum glass capillary. Initially, the self-closing is in the open state. Referring to Fig. 16B, when the user turns off the vacuum glass capillary in the vacuum pump 288a, an attractive force is generated to draw a predetermined amount of liquid into the capillary 284a. As the fluid passes through the self-closing valve 286a, the SAp in the self-closing valve 286a expands, thereby causing the self-closing valve 286a to enter a closed state, thereby preventing fluid from moving through the self-closing valve 286a. Similarly, by blocking the vacuum capillaries in vacuum pumps 288b and 288c, a predetermined amount of fluid can be drawn into capillaries 284b and 284c. The amount of fluid sucked into the capillary tubes 284a to 284c is determined by the volume of the capillary in the vacuum pumps 288a to 288c, and the amount of fluid sucked into the capillary tubes 284a to 284c may be the same or different. Referring to Fig. 17A, a device 29 for a two-step assay can be fabricated by using a combination of a vacuum pump, a disconnected open valve, and a self-closing valve. The two-step assay requires cleaning with a buffer after rapid binding of the reagent. One end of the passage 3〇2 is engaged to the sample cell 300 through the self-closing valve 296, and the other end of the passage 302 is coupled to the first vacuum pump 292a. Channel 302 is coupled to channel 308, which is coupled to buffer 298 via a disconnect open valve 294. Channel 302 is also coupled to channel 304, which is coupled to second vacuum pump 292b and third vacuum pump 292c. Channel 304 includes a binding and/or sensing region 306 that includes reagents for binding or sensing a compound in sample 3〇〇22 200813324 P64950010TWC1 22169-l-twf.doc/006 . Device 290 is operated in a manner such that sample 300 is aspirated toward bonding and sensing region 306 to cause a reaction to occur, followed by aspiration buffer 298 toward bonding and sensing £306 to clean the bonding and sensing regions. Referring to Figure 17B, vacuum pump 292a is activated to generate an attractive force that draws sample 300 toward vacuum pump 292a and draws sample 300 to a portion of passage 302 between vacuum pump 292a and self-closing valve 296. When the sample 3 〇〇 flows through the self-closing valve 296, a portion of the sample is attracted by the SAP in the self-closing valve 296, thereby bringing the self-closing valve 296 into a closed state. Referring to Figure 17C, the break open valve 294 is actuated to change the valve 294 to the open state. The vacuum pump 292b is activated to generate an attractive force for drawing the sample 300 and the buffer 298 toward the vacuum pump 292b. The vacuum mills 292a and 292b are designed such that after the pump is started, the sample 300 will stop at the bonding and sensing zone 306. After a period of time, vacuum pump 292c is activated to move sample 300 out of zone 306 and buffer 298 is flowed through zone 306 and wash zone 306. The above examples provide incubation time to allow for buffer 290 cleaning and bonding. The compound in sample 300 prior to sensing zone 306 is reacted with the reagent in zone 306. If the reaction at zone 306 is fast and the incubation time is unnecessary, vacuum pump 292b can be made larger and vacuum pump 292c can be omitted. When the vacuum pump 292b is activated, the sample rapidly flows through the bond and sensing region 306, and then the bond is cleaned by the buffer 298 and the sensing region 306 is shown in Fig. 18A, which can be opened by using a vacuum pump, a disconnected valve, and from 23 200813324 P6495001OTWC1 22169 Small twf.doc/006 A combination of a shut-off valve and a gas pump to make a device for a two-step assay. The 'two-step assay requires cleaning with a buffer after slowly combining the reagents. Device 310, similar to device 290, has a channel 302 that is connected to two channels 304 and 308. Channel 302 passes through self-closing valve 296 and is coupled to sample 300. Channel 308 is coupled to buffer 298 via a disconnect open valve 294. Channel 304 includes a bond and sense region 306. One end of the passage 304 is coupled to the break open valve 312. Gas pump 314 is coupled to buffer liquid 298. The difference between device 310 and device 290 is that device 310 does not use vacuum pump 292b to draw sample 300 and buffer 298 toward bonding and sensing region 306, but instead uses gas pump 314 to push sample 300 and buffer toward zone 306. 298. Referring to Figure 18B, in order to perform a two-step assay, vacuum pump 292 & is activated to draw sample 300 into the channel. Valve 296 enters a closed state after the sample has flowed through self-closing 阙 2%. Referring to Fig. 18C, the open type open valves 294 and 312 are actuated to cause the valve to be changed to an open state. Gas pump 314 is activated to generate gas over a period of time to push sample 300 and buffer 298 through homozygous and sense: ^ region 306. Since the gas pump 314 produces gas during a period of time (the reaction between the compounds of the raw gas and the gas takes a certain amount of time to pass through), the sample 300 can slowly pass through the bonding and the sensing region 3〇6, thereby slowly occurring. Conclusion: Yes. Further, with reference to Fig. 19A, the apparatus for three-step verification can be manufactured by adding the second buffer 324 and the structure shown in Fig. 17A to Fig. 17A, 24 200813324 P64950010TWC1 22169-l-twf. The doc/〇〇6 three-step assay requires two buffers to be washed after rapid reagent binding. To perform the multi-step assay, vacuum fruit 292a is activated to cause sample 300 to flow to channel 302. When the sample 300 flows through the self-closing valve 296, the valve 296 changes to the closed state. Referring to Fig. 19B, the break open valve 294 is actuated to change it to open and the vacuum pump 292b is activated to draw the sample 300 and the first buffer 298 toward the bond and sensing region 306. Referring to Fig. 19C, the open type open valve 326 is actuated to change to the open state' and the vacuum pump 292c is activated to draw the sample 300, the first buffer 298, and the second buffer 324 toward the bonding and sensing region 306. In this manner, the reaction at zone 306 can be washed with two different buffers. The means for verifying the steps of the three or more steps can be constructed by attaching additional buffers or samples and adding a corresponding number of vacuum pumps to the ends of the channels 304. Referring to Figure 20, a module 330 can be constructed to perform multiple analyte assays. The module includes a sample cell 282 for holding the sample 300 and three chambers 332a, 332b, and 332c, each chamber containing an analyte for binding and sensing a compound in the sample 300. The components used to perform the assay associated with the first analyte in chamber 332a are described below. Cavity 332a is flanked to sample reservoir 282 via passage 342a and self-closing valve 344a. The passage 342a is coupled to the first buffer 350a via a self-closing valve 346a and a disconnected open valve 348a. Channel 342a is coupled to second buffer 356a via self-closing two 352a and disconnected open valve 354a. The passage 342a is coupled to the third buffer 362a via a self-closing valve 358a and a disconnected open valve 36〇& to 25 200813324 P6495001OTWC1 22169-1 -twf.doc/006. The chamber 332a is also connected to vacuum pumps 33 such as 336a, 338a and 340a. To perform the assay, vacuum pump 334a is activated to draw sample 300 toward chamber 332a to allow the compound in sample 300 to react with analyte 332a. After a certain amount of sample flows through the self-closing valve 344a, the valve 344a changes to the closed state. The first buffer 350a is flushed through the chamber 332a by actuating the open type open valve 348a (changing the width to the open state) and the second vacuum pump 336a. After a certain amount of the first buffer 35〇a flows through the self-closing valve 346a, the valve 346a is changed to the closed state. The second buffer 356a is flushed through the chamber 332a by actuating the open type open valve 354a (changing the valve to the open state) and the third vacuum pump 338a. After a certain amount of the second buffer 356a flows through the self-closing valve 352a, the valve 352a is changed to the closed state. #, In a similar manner, the third buffer 362a is flushed through the chamber 332a by activating the open type opening valve 360a (changing the valve to the open state) and the fourth vacuum pump 340a. After a certain amount of the third buffer 362a flows through the self-closing valve j58a, the valve 358 is changed to the closed state. The person ~ can perform an assay associated with the chamber 332b and the second and third objects in the illusion, similar to performing the determination associated with the first analyte in the chamber 332a. Verifications associated with chambers 332a, 332b and the first, third, and third analytes can be performed simultaneously. = The application for vacuum pumps and gas pumps for performing biological tests. Figure ¥ π A and the figure shows that the 0 set 380 for performing the fast response colorimetric check includes the channel 384, and one end of the channel 384 is connected to 26 200813324 P64950010TWC1 22I69-l-twf.doc/〇〇6 tir end to Vacuum fruit 90. The sample cell 382 can hold the test after changing the color immediately after the compound == The sample in the sample cell 382 can be quickly sucked through the test zone 386.耩 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

23A and 23B show apparatus 390 for sampling filtered fluid. The device 390 includes a channel 384, a channel 384 (four) and another line. Job _== This pool 382. When the vacuum pump 90 is activated, the fluid (e.g., blood) in the sample cell 2 can be quickly aspirated through the filter membrane 392 to produce filtered fluid (plasma) that is drawn into the channel 384. Figures 24A through 24C show an apparatus 400 for a slow colorimetric assay. The device 400 includes a sample cell 402 coupled between the gas pump 404 and the channel 384. Channel 384 has a test zone 386 that has a test line that changes color immediately after detecting a compound. To use device 400, sample fluid 406 is placed in sample cell 402. A sealing strip 408 seals the opening of the sample cell. Gas pump 404 is activated to generate a gas that pushes sample fluid 406 through test zone 386. Because gas pump 404 produces gas over a period of time, sample fluid 406 travels through the test zone over a period of time, allowing for a slow colorimetric assay to be performed. Referring to Figures 29A and 29B, an antibody for the A liquid sample can be manufactured by using a combination of a self-closing valve (SLV) and a disconnected open valve (BOV). Calibrated device 27 200813324 P64950010TWC1 22169-1 -twf.doc/006 500. The device 5 includes a blood sample pool, ie, 00 (15 & 111 卩 16 \ 611 611) 5 〇 1, Washing buffer well 503, Metering zone and labeled antibody region 505 A Diagnostic Zone (antibody array) 507, a disconnected open valve (BOV) 509, a waste well 511, and a self-closing valve (SLV) 513. The metering region and marker antibody region 505 have a channel for communicating with the blood sample cell 501 and the flush buffer pool 5〇3, and a self-closing valve (SLV) 513 is located between the channels. The diagnostic method area 5〇7 has another channel, one end is connected to the center of the metering area and the marker antibody area 5〇5, and the other end is connected to the center of the marker opening valve (B〇V) 5〇9. Waste well 511. The apparatus 500 performs an antibody assay on a blood sample, as shown in Figs. 30A, 30B and 30C. First, the blood sample 502 is loaded into the blood sample cell 501, and then, using the self-closing valve (SLV) 513 proposed in the foregoing embodiment of the present invention, the capillary force is used to draw the liquid into the metering area and the mark. Within the channel of antibody region 505, 502a is shown in Figure 30A. Then, the rinsing buffer is loaded into the rinsing buffer tank 503, and at this time, the rinsing buffer also flows into the channel of the metering region and the marker antibody region 505, as shown in Fig. 3B, 504a, and the blood is squeezed at this time. Into the diagnostics area 507, as shown in Figure 502b. Then, referring to FIG. 30C, after the disconnected open valve (b〇V) 509 is disconnected, the blood is further drawn in through the diagnostic area 507, as shown in FIG. 502c, at this time in the diagnostic area 507. The internal antibody array will bind to the antigen in the medicinal solution (Antigent), while the rushing 28 200813324 P64950010TWC1 22169-l.twf.doc/006 wash buffer will flush the inactive blood to the waste pool 511 . Yet another embodiment of the present invention is shown in FIG. A device for performing an antibody assay on a blood sample can be made by using a combination of a self-closing valve (SLV) and a disconnected open valve (BOV). The device includes a blood sample cell, a flush buffer pool, a diagnostic zone (containing an antibody array), a disconnected open valve (BOV), a waste reservoir, and a self-closing valve. The blood water pool contains a thin membrane that removes blood cells (B 〇〇 J J , , , , , , , , , , , , , , , , , 血液 血液 血液 血液 血液 血液 血液 血液 血液 血液 血液 血液 血液 血液 血液 血液 血液 血液 血液 血液 血液 血液 血液 血液The gold liquid sample pool and the flush buffer pool are connected, and the other end is connected to the waste pool through the open open (B0V). The self-closing valve (SLV) is located before the blood sample pool enters the channel and can control the inflow. Diagnosing the amount of blood in the area and preventing blood from flowing back to the blood sample pool. When the disconnected open valve (BOV) is disconnected, the gold liquid is filtered through a membrane of removable blood cells and further inhaled into the diagnostic area. At this point, the antibody array in the #断法 region will bind to the antigen in the blood, and the flushing buffer will flush the inactive blood into the waste pool. Superabsorbent polymer ("SAP") can adsorb and maintain a large volume of water or other aqueous solutions. In some instances, SAP can be chemically modified starch and cellulose as well as other polymers. Poly(vinyl alcohol) PVA, poly(ethylene oxide) ruthenium, such as high hydrophilicity and high affinity for water. In some cases, the super sorbent polymer can be partially neutralized and lightly distributed. Made of poly 29 200813324 P6495001OTWC1 22169-1-twf doc/006 (acrylic), partially neutralized and lightly crosslinked poly(acrylic acid) with good performance versus cost ratio. 'And used in self-closing valves Super sorbent polymer, because the gelling time of the polymer is about several seconds, which is more suitable for vacuum pumping. When using Capillary Force and Centrifugal F〇rce, the fluid flow rate It is smaller than the gel time of SAP, so it only prevents the fluid from flowing and cannot allow the fluid to flow in the channel. Therefore, the application of the self-closing valve can be delayed by the design of the time delay layer. The "off" time of the valve, in addition, the retardation layer can be selected to not affect the material of the detection target, and can reach the application field suitable for slow flow rate, which is referred to herein as In this embodiment, a soluble material is disposed in the self-closing valve passage between the super adsorbent polymer SAP and the fluid, and when the detection solution flows through the passage, The rate at which SAP adsorbs and maintains the bulk of water or other aqueous solutions is retarded by the soluble material and the rate of swelling of the gel slows down, which results in slow flow rates. • Tea See Figure 32A, self-closing valve 550 A channel 552 is included that has an enlarged portion 554 to accommodate the super sorbent polymer 556 such that the super sorbent polymer 556 does not restrict the flow of fluid prior to expansion. In order to manufacture the self-closing width 550, an adhesive agent may be applied to the inner wall of the enlarged portion 554, and the SAP 556 in powder form is then pushed into the channel 552, so that the SAP 5S6 powder adheres to the inner wall at the enlarged portion 554. A retardation layer 558 made of a corrugated material can be placed in the passage of the fluid passage of the passage 552 in the enlarged portion 554. Referring to Fig. 32β, when the fluid is in circulation 30 200813324 P64950010TWC1 22169-l-twf.doc/〇〇6, the super adsorbent polymer MS must start to adsorb the part of the fluid and expand in volume after the dissolution of Yanxian 558. Blocking the passage 552, thereby preventing the fluid from flowing further through the expanded aggregate. The selection of the material of the retardation layer is mainly based on the soluble material. In one embodiment, the water-soluble material may be selected, for example, a sugar block. (zero rcube) or sugar sheet, the thickness is between 〇〇1~2, and it is covered with rogue and super (four) age compound (SAP). #水丝体流流流流's first action with the rhyme to prevent the absorption of water by SAp, when the sugar is dissolved - (4) "allows SAp - while absorbing water. When the sugar bath is dissolved, 'SAP side Can completely block the flow channel and complete the "close," action. This extension of bribes (4) is increased by the increase in thickness, which can be calculated according to the demand. In addition, the choice of different retardation layer materials will have different delay time effects. In other embodiments, the passage from the valve 55A says that the enlarged portion 554' may not be required and instead the supersorbent polymer 556 powder may be adhered to the inner wall at the passage 552, and the super adsorbent polymer 556 Powder can be #SAP material fixed after 'dip-eGating of molten syrup (dlp-eGating), drum coating (Rq11 c (10) (four) or spin coating (Spin Coating) and other processes 'to laminate the layer in the super adsorption In the powder of the polymer 556, as shown, for example, in Figures 33A and 33B, respectively, the coating of the partial coating or the full coating is shown. At this time, the velocity of the large volume of the SAp material adsorbed and maintained or other aqueous solution will be delayed. This also achieves this purpose. The above-described time delay valve design can be used in a number of examples, for example, 31 200813324 P6495001OTWC1 22169-1-twf.doc/006 The apparatus for the antibody assay depicted in Figures 34A and 34B. In the antibody assay device 600, a sample flow channel 610 and a labeled labeled antibody well 620, a sample well 630, and a Washing Buffer Well 640 are included. There are two time delay valves 622 and 632 respectively between the sample flow channel 610 and the labeled antibody tank 620 and the sample tank 630. The sample flow channel 610 includes a step-by-step region 612 and an antibody array region (Antibody Array Area). 614 and Broken Open Valve 616. This step area 612 is mainly designed with the time delay valves 622 and 632. The flow path diameter of the sample flow passage 610 is as shown in Fig. 34B along the sectional view AA. 'The step design shown is changed from the larger runner diameter 611 to the second runner diameter 613 and then to the smaller runner diameter 615. The design of the tapered runner diameter can be Effectively increase the size of the downstream capillary force. With the step design, with the use of the time delay valves 622 and 632, when the liquid tank flows into the specific pipeline section, the liquid volume function can also be achieved. In the antibody assay method of the antibody assay device 600, in one embodiment, an antibody assay is performed on, for example, a liquid sample A, as shown in Figures 35A, 35B, 35C and 35D. The antibody assay device 600 includes a sample flow channel. 610, labeled antibody tank 6 20. Sample tank 630, rinse tank 640, first time delay valve 622 and second time delay valve 632. Sample flow path 610 includes step area 612, antibody array area 614 and open open valve 616. This step area 612 includes areas 611, 613 and 615 of different flow path diameters. The above region hi has a larger diameter of the flow path of 32 200813324 P64950010TWC1 22169-l-twf.doc/006, and the area 613 has a smaller flow path diameter, for example, less than the flow path control of the above-mentioned area 611. First, as shown in FIG. 35A, the blood sample 635 is loaded into the sample well 630, and then the blood sample 635 is slowly flowed to the region 613 via the second time delay valve 632, followed by the second time delay valve 632. The blood sample 635 will no longer flow into the sample flow channel 610. Since the diameter of the flow path in the region 613 of the sample flow channel 610 is fixed, a blood sample 635 of a fixed volume can be obtained. Then, as shown in Fig. 35B, the labeled antibody 625 is loaded into the labeled antibody tank 620, and then the labeled antibody 625 is slowly flowed to the region 611 via the first time delay valve 622, and then the first time delay valve 622 will be turned off and the labeled antibody 625 will no longer flow into the sample flow channel 610. On the other hand, since the flow path diameter is fixed in the region 611 of the sample flow channel 610, a fixed volume of the labeled antibody 625 can be obtained. Then, as shown in Fig. 35C, the rinsing liquid 645 is added to the rinsing liquid tank 640, and then, as shown in Fig. 35D, after the disconnecting opening valve 616 is broken, the generating and attaching force 'will be in the sample flow passage 610. The rinsing solution 645, the labeled antibody 625 located in the region 611, and the blood sample 635 located in the region 613 are adsorbed and passed through the antibody array region 614 to achieve the effect of antibody assay. In the above antibody assay method, the flow path diameter in the sample flow channel is tapered to increase the downstream capillary force, and is designed in steps, with a time delay valve, when the liquid tank flows into a specific pipeline section to reach a liquid Fixed volume function. The sample is sequentially injected into the sample tank, and the labeled antibody is labeled at 33 200813324 P6495001OTWC1 22169-1 -twf.doc/006. After the antibody buffer and the rinsing liquid are labeled in the rinsing liquid tank, the open type open valve is opened, and the liquid is caused by capillary force. Drive, sequentially flow through the antibody array area, and perform binding, labeling, and rinsing procedures. The above-described time delay valve design can also be applied to a device for performing blood cell separation by centrifugal force as shown, for example, in Figs. 36A and 36B. In the whole blood separation device 660 in which the time delay valve is disposed in Figs. 36A and 36B, a whole blood separation tube 662 is included. A time delay valve 664 is disposed on the inner side wall of the whole blood separation tube 662. The whole blood separation tube 662 is rotated according to a rotation axis 666, and a centrifugal force is generated in a centrifugal direction 668 (Centrifugal Force). In the whole blood separation tube 662, a Whole Blood Sample 661 is injected, and when the high-speed centrifugation is performed, the jk ball of the whole sample 661 is concentrated in the centrifugal region to generate a Blood Cell Area 663. And plasma zone (Blood Plasma

Area) 665. During high-speed centrifugation, the water-soluble retardation layer of the time delay valve 664 is dissolved, and after being separated, the superabsorbent resin (SAP) absorbs part of the water and then expands, and blocks the blood cell region 663 and the plasma region 665. Diffusion mixing between. Some examples have been discussed above, but other implementations and applications are also within the scope of the following patent application. For example, in Fig. 1A and Fig. 2, the container 1〇〇 may contain a low pressure region rather than a vacuum region. As long as the gas pressure inside the vessel 100 is lower than the gas pressure of the vessel 1 outside the vessel, when the Gu 100 is disconnected, the pressure in the region (10) outside the vessel (10) will drop and the attraction will be induced toward the vessel in one direction. The attraction of fluids. The capillaries described above may be replaced by capillaries made of other brittle materials 34 200813324 P64950010TWC1 22169-I-tw£doc/006, such as brittle plastic, quartz [simplified illustration] ~ - Figure 1A and Figure 1B Schematic diagram of the vacuum pump. 2A and 2B are not intended to be gas fruits. 3A and 3B are schematic views of a gas pump. 4A is a schematic view of a gas pump. Figure 4B is a table of materials. Lutu 5A and Fig. 5B are schematic views of a disconnected open valve. 6A, 6B, 7A, 7B and 8A to 8C are schematic views of the self-closing valve. _ Figures 9A to 9C are schematic views of the valve opening and closing. 10A to 10C are schematic views of the switch-off valve. 11A to 11D are schematic views of a switch-on/off valve. Figure 12 is a schematic illustration of a metering straw. Figure 13 is a schematic illustration of a metering straw. 14A to 14C are schematic views of a metering straw. Lu Figure 15A and Figure 1 SB are schematic views of the metering device. 16A and 16B are schematic views of a metering device. 17A to 17C are illustrations of the apparatus for the two-step assay - Figs. 18A to 18C are diagrams showing the apparatus for the two-step assay. 19A through 19C are schematic diagrams of a sound map 20 for a three-step assay device for a module for multiple analyte assays. W Figure 21A and Figure 21B show a metering straw being used to sample blood from a patient. 35 200813324 P6495001OTWC1 22169-l-twf.doc/006 Figures 22A and 22B are schematic diagrams of an apparatus for performing a fast-reaction colorimetric assay.

2A to 2SC are schematic views of a vacuum pump. 26A and 26B are schematic views of a vacuum pump. 27A to 27C are schematic views of a self-closing valve. 28A and 28B are schematic views of a disconnected open valve. Cross-section. Figure 28C shows a glass capillary having a square inner perimeter and an outer perimeter. Figures 29A and 29B illustrate the use of a combination of a self-closing valve (SLV) and a break-open valve (Β〇ν) for the fabrication of the embodiment of the present invention. The liquid sample is subjected to an antibody (antibody) assay device. Figures 30A, 30B and 30C illustrate a schematic view of the operation of the blood test device for the blood sample of Figure 29A. Figure 31 illustrates a device for making an antibody assay for a blood sample by using a combination of a self-closing valve (SLV) and a disconnected open valve (Β〇ν) in accordance with another embodiment of the present invention.彳 Figures 32A and 32B are schematic views of a self-closing valve having a hysteresis layer. Figures 33A and 33B show a partial coating or a full coating, respectively. Figures 34A and 3BB illustrate an embodiment of the antibody assay device of the present invention having a time delay valve. 36 200813324 P6495001OTWC1 22169-1 -twf.doc/006 Figures 35A, 35B, 35C and 35D illustrate an antibody assay method of the present invention having a timed valve. Figures 3 6 and 3 6 B illustrate an embodiment of a liquid centrifuge device having a time delay valve as proposed by the present invention. ^ [Main component symbol description] 90 · Vacuum pump 92: Gas pump 94: Gas pump 96: Gas pump 100: Container 102: Material 104 · · Area 106: Channel 108: Direction 110: Container 112: Area • 114: Direction 120 : glass capillary Λ 124 : channel ★ 126 : first material 128 : second material 130 : compound 132 : glass capillary 140 : open type open valve 37 200813324 P6495001OTWC1 22169-1 -twf.doc / 006 142 · · glass capillary 144 Open end 146: closed end 148: first passage "150: second passage 152: second passage 160: self-closing valve 162: super adsorbent polymer (SAP) 164: passage 166: first region 168: second Zone 170: self-closing valve 172: enlarged portion 180 • wire 182 • recessed area 190: switch open valve • 210: switch on and off valve 212: super adsorbent polymer (SAP) ~ 214: super adsorbent polymer (SAP) , 220: metering straw 222: metering straw 224 · straw tube 226: sphere 228: tube body 38 200813324 P6495001OTWC1 22169-1 -twf.doc / 006 230: straw 232: gas pump 234: straw sphere 240: metering straw ^ 242: Sphere 244: Middle Section 246: Suction Tube Body 248: Suction Tube Body • 260: Metering Device 262 • Glass Capillary 264: Section 266a: Branch 266b: Branch 268a: Self-closing Valve 268b: Self-closing Valve 270a: Disconnected Open Valve • 270b • Disconnected open valve 274: Fluid • 280: Metering device, 282: Sample cell 284a: Capillary 284b: Capillary 284c • Capillary 286a: Self-closing valve 39 200813324 P6495001OTWC1 22169-1 -twf.doc/006 286b : self-closing valve 286c: self-closing valve 288a · vacuum 288b: vacuum valve Λ 288c · vacuum valve 290: device 292a: first vacuum pump 292b: second vacuum pump® 292c: third vacuum pump 294: open-open valve 296: self-closing valve 298: buffer 300: sample cell 302: channel 304: channel 306: bonding and/or sensing zone • 308 • channel 310: device Λ 314: gas pump. 320: device 322: channel 324: Second buffer 326: open type open valve 330: module 200813324 P64950010TWC1 22169- l-twf.doc/006

332a: chamber 332b: chamber 332c: chamber 334a · vacuum pump 336a: second vacuum pump 338a: third vacuum pump 340a: fourth vacuum pump 342a: channel 344a: self-closing valve 346a · self-closing valve 348a: disconnected Open valve 350a: first buffer 352a: self-closing valve 354a: open type open valve 356a: second buffer 358a: self-closing valve 360a · disconnected open valve 362a: third buffer 370: blood 380: Device 382: sample cell 384: channel 3 8 6 · test zone 390: device 41 200813324 P6495001OTWC1 22169-1 -twf.doc/006 392: filter membrane 400: device 402: sample cell 404: gas pump ^ 406: sample fluid 408 : sealing tape 410 : gas pump 412 : gas pump 416 · vacuum glass capillary 418 : tube 420 · rubber tube 424 : closed end 426 : open end 428 : closed end 430 : vacuum pump 432 : vacuum pump 10 434 : planar substrate 436 : Elastic layer • 438 : fluid channel. 440 · · closed end 442 : open end 444 : fluid channel 450 : wire 460 : plastic channel 200813324 P6495001OTWC1 22169-1 -twf.doc / 006 462 : plastic channel 464 : reagent 466 : UV Light source 468: detector 5〇 〇: Device for antibody testing of blood samples 5〇1, Blood sample well 503 · Washing buffer well 505 · Metering zone and labeled antibody Area 507 · Diagnostic Zone (antibody array) 509 : Disconnected open valve 511 : Waste well 513 : Self-closing valve (SLV) 550 · Self-closing valve 552 · Channel 554 β•Expanded portion 556: Super adsorbent polymer 558: retardation layer 600: Antibody assay device 610: Sample flow channel 612: Step region 614 • Antibody array region (Antib〇dy Array Area) 616 • Disconnected open Valve (Broken Open Valve) 43 200813324 P6495001OTWC1 22169-1-twf.doc/006 620 : Labeled Antibody Well 630 : Sample Well 640 : Washing Buffer Well 622 : First Time delay valve 632 · Di two time delay valve 625 · Standard § antibody 635 · · Gold sample 660 : Whole blood separation device 662 with time delay valve · · Whole blood separation tube 664 : Time delay valve 666: Rotary shaft 668 · Centrifugation Forcerifugal Direction 661 · Whole Blood Sample 663 : Blood Cell Area 665 · Bi〇〇d Plasma Area

44

Claims (1)

200813324 P649500K)TWC1 22169 small twf.doc/006 X. Applying for a patent garden: 1· A method consisting of: controlling the smuggling of fluids in the channel, ^ the pressure difference generated in the channel makes the household - the air seam force outside the container' The material = before the first container is disconnected and the second =: t first material and the second material is selected to interact with the second material immediately after production - 2 by the patent application The method of the present invention, wherein the first space has a higher pressure than the pressure outside the first container. 3. The method of claim 2, wherein the towel controls the fluid The fluid is contained in the bypass shaft of the fluid that is remote from the first container that is broken. The method of claim 1, wherein the space in the first container has a pressure lower than the pressure outside the first container. The method of claim 4, wherein controlling the flow of the fluid comprises attracting the fluid in the channel to the first container that is broken. 6. The method of claim 5, wherein the fluid 45 200813324 P6495001OTWC1 22169-l-twf.doc/006 comprises blood and controlling the flow of the blood comprises passing the blood A filter blocks blood cells and allows plasma to pass through the filter and into the channel. 7. The method of claim 5, further comprising performing a colorimetric assay when the fluid flows in the channel. 8. The method of claim 5, further comprising a second container, the second container (a) defining a space within the second container, the space having a higher than the second container a gas pressure of the gas pressure, or (b) comprising a third material, the third material being separated from the fourth material before the second container is disconnected, the third material and the fourth material being selected The gas is generated immediately after the third material interacts with the fourth material. 9. The method of claim 8, wherein controlling the flow of the fluid comprises pushing the fluid in the channel away from the second container. 10. The method of claim 5, wherein controlling the flow of the fluid comprises preventing additional fluid from moving in a direction by using a fluid adsorbing material that expands immediately after the portion of the fluid is adsorbed. And through the channel. 11. A fluidic device comprising: a passage; and a first container that creates a pressure differential in the passage when the first container is disconnected, the first volume being made of a brittle material, Wherein the first container (a) defines a space within the first container, 46 200813324 P64950010TWC1 22169-l-twf.doc/006 the chamber has a gas pressure body pressure different from the outside of the first container, Or (b) comprising a first material, the first material being separated from the second material before the device is disconnected, the first material being selected by the first material 以 such that the first material The gas is produced immediately after the interaction of the first ancestors. The fluid device No. H'a, H(a), as defined in the application of the fluid device, defines that the space in the second container has a gas pressure different from the outside of the second container; The pressure, wb) includes a third material that is separated from the fourth material by the rain at the third, and the third material and the material are selected such that the third material The gas is generated immediately after the first:. a paste interaction 13. The fluid of claim 12, wherein the space in the first container has a pressure of a higher pressure, and the empty space in the second container The pressure of the pressure outside the second container. - 14. The application of the full-time _ 12 described in the description of the first Μ Μ 魅 魅 有 有 有 有 有 魅 魅 魅 魅 魅 魅 魅 魅 魅 魅 魅 魅 第二 第二 第二 第二 第二 第二 第二 第二 第二 第二 第二 第二 第二 第二 第二= - 枓 与 与 与 与 与 与 枓 枓 第二 第二 第二 第二 枓 第二 第二 第二 枓 枓 枓 枓 枓 枓 枓 枓 枓 枓 枓 枓 枓 枓 枓 枓 枓 枓 枓 枓 枓 枓...,, ..., ^, as in the fluid device described in claim 14, the membrane is filtered, and the membrane is used to bind blood cells and allow plasma material. ^ 5 47 200813324 P64950010TWC1 22169-l-twf.doc/〇〇6 The melons are the fluids of the U.S. patents, and the secret materials include at least one of the machine materials: quartz, glass, and jin. Ίίί: The two or more of the complex, glass, ceramic and plastic, such as the fluid passage described in item 11 of the U-Li range is made of a flexible material. The fluid device described in item n of the middle material is a material containing gas generated when heated. The fluid-reported container described in claim n, wherein the container comprises a material that is sublimated from a solid to a gaseous state upon heating, comprising: a first material defining a first region; The second material of the second region is separated from the first region; the connector is connected to the domain = the brother: =, the connector contains the secret material = is opened: the second end is placed in the second And the passage of the first region to the second region when the closed end of the closure is disconnected from the closed end of the second region partitioning connector. 21. A fluidic device comprising: - comprising an expanded portion (four) track - said expanded portion having a diameter greater than said adjacent portion; and /, 'material disposed in said expanded portion, said material Having a volume that does not block the passage of the fluid prior to the attachment of the fluid, wherein the material adsorbs a portion of the fluid and immediately expands to cause expansion. The material blocks the passage of additional fluid through the channel. The fluid device of claim 21, wherein the material comprises a super adsorbent polymer. 23. A fluid device comprising: a first fluid containing reservoir, a second reservoir containing a second fluid; a primary channel; a first branching channel, the first branching channel a first reservoir to the main channel; a second branch channel, the second branch channel is lightly connected to the second reservoir to the main channel; a first single use pump, when The first single use pump generates a pressure differential to move one or both of the first fluid and the second fluid when the first single use pump is disconnected; and • a second single Using a pump, the second single use pump creates a pressure differential to move one or both of the first fluid and the second fluid when the vessel in the second single use pump is disconnected . The fluid device of claim 23, wherein the first container (a) defines a space within the first container, the space having a gas pressure different from that of the first container a gas pressure, or (b) comprising a first material, the first material being separated from the second material prior to the first container being disconnected, the first material and the second material being selected to be 49 200813324 P64950010TWC1 22169-l-twf.doc/〇〇6 ^The gas material is generated immediately after the interaction of the first material with the second material. The fluid device according to claim 23, further comprising ^ II, the valve '34 self-closing valve includes a material that initially has a small volume such that the chiropractor passes through the valve, the material increases in volume after adsorbing the portion of the body to prevent The fluid further passes through the passage of the valve. The fluid device of claim 23, further comprising: the valve having a connector made of a brittle material, wherein the valve blocks the first when the connection is intact When fluid enters the main passage I and the connector is disconnected, a passage is created to allow the first fluid to enter the main passage. 8. The fluid device of claim 23, further comprising: a second reservoir, the third reservoir containing a third fluid; and a first branch channel, the third The branching channel is coupled to the third reservoir to the main channel. The fluid device of claim 23, further comprising a sensing region, the sensing region being coupled to the main channel, the sensing region comprising determining whether a specific material exists in the A sensor in the first fluid. 29. A method comprising: providing a plurality of straws to enable sampling of a predetermined amount of fluid, each straw comprising: a channel, and a container, the container creating a pressure differential in the channel when disconnected, 50 200813324 P64950010TWC1 22169-l-twf.d〇c/〇〇6 The container is made of a brittle material that defines a space within the container. The space has a gas pressure that is less than the gas pressure outside the container. 'Disconnecting the container creates a predetermined amount of pressure differential in the passage such that a predetermined amount of fluid is drawn into the passage. 30. A method comprising: enabling fluid to flow from a first region to a second region, the first region being coupled to the second region of the Lu by a connector having an open end and a closed end, the opening The end is disposed in the second region, and the closing step is placed in the region of the brother, the first region being separated from the second region by the closed end of the connector, wherein Having the fluid capable of flowing includes breaking the closed end of the connector to form a passage from the first region to the second region through the connector; expanding by volume after use of the adsorbing fluid Material to adsorb a portion of the fluid flowing through the connector; and use the expanded material to block additional fluid from flowing further through the connector. 31. A method comprising: flowing a fluid in a channel, the channel comprising a material that adsorbs a portion of the fluid immediately after the volume expands, causing the fluid to flow in the channel to include the fluid a portion flows through the material and uses the material to adsorb a second portion of the fluid such that the material expands in volume; and blocks additional fluid passing through the use of the swelled material 51 200813324 P6495001OTWC1 22169-1 -twf.doc/006 The path of the channel. See, as described in claim 31, further comprising a disconnecting end of the connector to enable additional fluid to flow through the connector by bypassing the fluid through the expanded material Flowing in the channel, wherein the connector has an open end disposed in the first portion of the channel and a closed end disposed in the second portion of the channel prior to opening the closed end, The first portion and the second portion are separated by the material of the expanded material. The method of claim 32, further comprising adsorbing a portion of the fluid flowing through the connector by using a material that expands in volume after adsorbing the fluid, and using the expanded The material blocks the additional fluid from flowing further through the connector. The method of claim 31, wherein the material comprises a super adsorbent polymer. 35. A method comprising: passing a fluid through a passage, the passage including a first self-closing valve and a self-closing valve 'the self-closing valve and the second self-closing interval are spaced apart from each other' Each self-closing valve includes a fluid adsorbing material that immediately expands after the portion of the fluid is adsorbed; and the fluid adsorbing material is adsorbed by using the first self-closing valve and the second self-closing valve a portion of the fluid; and expanding the volume of the fluid adsorbing material to block passage of additional fluid further through the passage, the first self-closing valve and the second self-closing valve of the passage The predetermined amount is maintained in the portion between 52 200813324 F64950010TWC1 22169-l-twf.doc/〇〇6 fluid. 36. The method of claim 35, further comprising using capillary forces to draw the fluid through the passage. 37. — A method that includes: Blocking a first container made of a brittle material to create a pressure differential in the passage such that the first fluid moves from the first reservoir to the first section of the passage, the first container (a) defining a space within the first container, the space having a gas pressure different from a gas pressure outside the first container, or (b) including a first material, the first material being opened in the Separating from the second material, the first material and the second material are selected such that the first material interacts with the second material to generate a gas immediately; and, the hindrance is made of a brittle material The second container creates a pressure differential in the passage such that at least a portion of the first fluid moves past the second section of the passage. The method according to Item 37 of the monthly production (4), the application of the product (4), further comprises blocking the first valve made of the continuous material to generate the first passage, the first passage connecting the ang-reservator to the passage, The second reservoir includes a second flow-breaking method, wherein the second fluid is self-blocking to move to the channel by blocking the pressure difference of the second=2 The second section. 40. The second container made of brittle material, as described in item 5% of the scope of the patent application, has a poor productivity, so that 53 200813324 r 〇4y i OTWC1 22169-1 -twf.doc/0〇6 The second store (four) moves to the second hidden segment of the channel. 41. The method of claim 4, further comprising limiting a second valve made of a brittle material to create a second passage, the passage connecting the second reservoir to the passage, The third reservoir contains a third fluid. 42. The method of claim 41, further comprising cleaving a third container made of a brittle material to create a pressure differential such that the third fluid moves from the second reservoir to the The second hidden segment of the channel. The method of claim 37, wherein the second section of the channel comprises a sensing agent for determining whether a particular mosquito material is present in the first fluid. The method of claim π, wherein the space in the first container of the first boundary f, the space has a gas pressure lower than a gas pressure outside the first volume n. 45. The method of claim 44, wherein the space in the second container is a gas pressure that is lower than a gas pressure outside the gas. The method of claim 44, wherein the space of the seventh (the fourth) said volume (4) has a gas pressure κ 虱 body pressure (a) higher than the gas pressure of the second volume, or (8) comprising a first material, the first-grain state and the second material phase separation material: = said, the material is selected such that the first material is converted to a gas after the true action.枓 枓 54 200813324 ro^fy juu iOTWC 1 22169-1-twf.doc/006 47·—A method consisting of: 2 operation of the first-single pump and the second single: the fruit is not sucked = this:! It! Part of the first channel to the first channel and the sample fluid.刀刀至弟—forcing' includes blocking the first container of the first_single= to generate a pressure difference, such that the brother-portion of the sample fluid moves from the reserve to the first channel, and the wire is broken The first a second container of itnt to create a pressure difference such that the center of the sample body is from the collector shaft to the second passage; and simultaneously operating the third single money pump and the fourth single money pump The second channel is dissolved by extracting the first channel from the first buffer solution and sucking the second buffer. 48. The method of claim 47, further comprising simultaneously performing a fifth single use pump and a sixth single use to draw the third buffer solution to the first channel and to absorb the fourth buffer Listen to the second channel. , -49·, the method described in claim 47 of the patent scope, further includes the fifth-order operation of the same-single-single-mode pump simultaneously: owing to avoid (four) taking the third part of the sample fluid to the first Three channels, and a sixth single use pump is operated simultaneously with the third single use pump to draw a third buffer solution to the third channel. A fluid device comprising: a channel including an expanded portion, the expanded portion having a diameter greater than an adjacent portion of the channel; , 55 200813324 ^〇4^uui0TWCl 22169-Mwf.doc/0〇6 female a material disposed in the expanded portion; and the flow-delay material disposed between the material in the expanded portion and the passage of the body, wherein the material is placed in the expansion after the material is dissolved The portion 1 of the material has a volume that does not block the passage of the fluid prior to adsorbing the fluid, and the portion of the fluid is immediately swollen so that the material blocks additional fluid after expansion. Said channel road. The fluid device of claim 5, wherein the material comprises a super adsorbent polymer. 52. The fluid device of claim 5, wherein the retardation material is a water soluble material. 53. The fluidic device of claim 50, wherein the stagnant material is a sugar cube or a sugar sheet. 54. A fluid control method comprising: flowing a fluid in a passage, the passage comprising a material that adsorbs a portion of the fluid immediately after expansion of the volume, and a material disposed between the material and the fluid passage a retardation material, after the retardation material is dissolved, the material placed in the expanded portion has a volume that does not block the passage of the fluid before adsorbing the fluid, wherein the material adsorbs a portion of the fluid The volume expands immediately so that the material blocks the passage of additional fluid through the passage after expansion. 55. The fluid control method of claim 54, wherein the material comprises a super adsorbent polymer. 56. The method of controlling a fluid according to claim 54, wherein the retardation material is a water-soluble material. 57. The fluid control method of claim 54, wherein the latent material is a sugar cube or a sucrose red sheet. 〇58. The fluid control as described in claim 54 The method wherein the stagnant material disposed between the material and the fluid pathway is formed using a dip-coating. The fluid control method according to claim 54, wherein the retardation material disposed between the material and the fluid passage is formed by Roll coating. The fluid control method of claim 54, wherein the retardation material disposed in the fluid passage is formed using a spin coating method. The fluid control method of claim 54, wherein the material and the retardation material of the fluid passage are partially coated or fully coated. 62·------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ a flow path to the sample; and a flushing liquid tank connected to the sample flow channel, wherein the test 57 200813324 i OTWC1 22169-1 -twf.doc/006 includes a step-by-step region a body array region and a break open valve, wherein the flow path of the step region is designed as a tapered flow tube tube, wherein the first and second time delays comprise a water swellable material and a a water-soluble retardation layer, wherein after the water-soluble retardation layer in the time-delay valve is dissolved, the water-swellable material in the time-delay valve swells to prevent the labeled antibody tank and the sample tank from being Diffusion mixing between the sample flow channels. 63. An antibody assay method, which is suitable for an antibody assay device, wherein the antibody assay device includes a sample flow channel, wherein the flow path of the sample includes a flow tube diameter that is a step of a tapered flow path design. a step area, the method comprising: flowing the same flow through a second time delay valve to a flow path diameter of the tapered flow path, and then the second time delay valve is closed; a labeled antibody flows through a first time delay valve to a larger flow path diameter area within the tapered flow path diameter, wherein a flow path diameter of the larger flow path diameter area is greater than the secondary flow path tube a flow path diameter of the radial region, and then the first time delay valve is closed, wherein the first and second time delay valves comprise a water absorbing material and a water-soluble delay lag and the time delay After the water-soluble retardation layer in the stagnation is dissolved, the water-swellable material in the time-delay valve will swell and prevent the labeled antibody and the sample from diffusing into the sample flow channel; Into the sample flow channel And using an adsorption force to adsorb the rinsing liquid into the sample flow passage 58 200813324 roH^juuiOTWCl 22169-1 -twf.doc/006, and through the secondary flow path diameter area and the larger flow In the tube diameter region, the blood sample in the secondary flow path diameter region and the labeled antibody in the larger flow channel diameter region flow through an antibody array region to complete the antibody assay. 64. The antibody assay method according to claim 63, wherein the water-soluble retardation layer material is a water-soluble material. 65. The method for assaying an antibody according to claim 63, wherein The water > cereal retardation layer material is a SUGAR cube or a sugar sheet, a whole blood separation device, including: a whole blood separation tube, and the whole Configuring a time delay valve on the inner side wall of the blood separation tube, wherein the time delay valve comprises a water swellable material and a water-soluble retardation layer; and a rotating shaft, the whole blood separation tube according to the rotating shaft Rotating, generating a centrifugal force in a centrifugal force direction, and a whole blood sample in the whole blood separation tube is subjected to centrifugation to generate a blood cell region and a plasma region, and the time delay valve is water-soluble. After the retardation layer is dissolved, the water swellable material in the time delay valve will swell and prevent diffusion mixing between the blood cell region and the plasma region. The whole blood separation device according to claim 66, wherein the water-soluble retardation layer material is a water-soluble material. /, ,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,