TW200819136A - Antrodia comphorata polysaccharides with hepatoprotective efficacy - Google Patents

Abstract

This invention relates to a composition for Intravenous (IV) lnjection injection comprising a neutral polysaccharide of Antrodia camphorata having characteristics as follows: (a) appearance: colorless and shapeless powder, (b) pH: neutral, (c) molecular weight: 899.5-1670.5 kDa determined by HPLC as shown in Figure 4, (d) rotatory power: [α] D +115.0 DEG (c=0.4433, H2O), (e) intrinsic viscosity: [η]=0.03971~0.06255.g-1, (f) specific heat Cp: 0.2536~0.3995 Cal/g. DEG C, (g) IR spectrum: as shown in Figure 5, (h) 1H-NMR spectrum: as shown in Figure 6, (i) 13C-NMR spectrum: as shown in Figure 7, and (j) GC-MS analysis: as shown in Figure 8.This invention relates to a composition for oral administering comprising a neutral polysaccharide of Antrodia camphorata having characteristics as follows: (a) appearance: colorless and shapeless powder, (b) pH: neutral, (c) molecular weight: 899.5-1670.5 kDa determined by HPLC as shown in Figure 4, (d) rotatory power: [α] D +115.0 DEG (c=0.4433, H2O), (e) intrinsic viscosity: [η] = 0.03971~0.06255 dl.g-1, (f) specific heat Cp: 0.2536-0.3995 Cal/g. DEG C, (g) IR spectrum: as shown in Figure 5, (h) 1H-NMR spectrum: as shown in Figure 6, (i) 13C-NMR spectrum: as shown in Figure 7, and (j) GC-MS analysis: as shown in Figure 8.

Description

[Technical Field] The present invention is a composition containing an anthraquinone neutral polysaccharide which can be used for an injection or an oral administration. [Previous technique] Antrodia camphorata or Antrodia cirmamomea is a genus of the genus Antrodia of the family poly-poraceae (Aphyllophorales), also known as Antrodia camphorata or Bovine oyster mushroom, which is parasitic in the tree hole of Taiwan's endemic burdock tree. The fruiting bodies of Antrodia can be formed all year round and have a strong odor. The appearance is not the same as other Ganoderma lucidum, and it is in the shape of a disk or a bell. The growth pattern of Chiba is fixed in the cork layer inside the burdock tree hole. The surface of the disc-shaped Antrodia camphora is orange-orange/orange, which is spread over small holes, while the bottom is yellow-white cork. The body layer of the bell-shaped stick (the bell-shaped surface) is also orange/orange, which is filled with small holes containing spores. These grips have a bitter taste and are orange-red in the fresh state, and then turn into orange-brown or brown. The 4-meridian fruit 疋 _ layer looks like a dark green-brown shell, which looks smooth and transparent under the microscope, showing a slightly curved column. Antrodia is traditionally used to treat poisoning caused by food, alcohol or (four) substances, as well as abdominal, monthly pain, south blood pressure, skin blemishes and cancer (shen et this, 2〇〇4, fems he bumps

Lett” 23h 137-143). In the past, studies on phytochemicals have isolated many new steroid quinones, dioxins and polysaccharides _ lean (Su, Hc〇i·a ie L

Pharmacol 201: 186-193). 6 200819136 Polysaccharides are structural or storage polymers commonly found in organisms, accounting for more than 75% of the dry weight of plants. The analysis of glycosidic structure of these polysaccharides is important in composition analysis. Polysaccharides may be medicinally effective because they have many biological activities, such as stimulating cell division, initiating alternative biochemical pathways, and plasma agglutination (Lee et al., 2002, FEMS Microbiol. Lett., 209: 63-67). Chen et al., 2005, Life Sciences, 76: 3029-3042). The polysaccharide isolated from Antrodia sinensis has anti-B hepatitis virus, anti-inflammatory, anti-angiogenic and anti-tumor functions (Lee et al., 2002, FEMS Microbiol. Lett., 209: 63-67; Shen et al. , 2004, FEMS Microbiol. Lett" 231: 137-143; Chen et al., 2005, Life Sciences, 76: 3029-3042; Lieu et al.? 2004, Toxicol. Appl. Pharmacol 201: 186-193), However, the composition, structure and other characteristics of Ganoderma lucidum polysaccharides have not been understood. Hepatitis is a common disease in developing countries, but there are no effective drugs available for treatment. In recent years, scientists have completed good traditional medicines. Research, trying to find a cure for cakes, some compounds can improve immunity, fight against toxic substances, so that liver cells do not cause necrosis or improve the damage of liver cells, they may have therapeutic potential SUMMARY OF THE INVENTION The present invention provides a composition containing a neutral medicinal herb, which can be used as an injection, and has the following characteristics: (4) Appearance · Colorless and no shape powder. (6) Acid value : Neutral. (C) Molecular weight: as shown in Figure 4, analyzed by high-performance liquid chromatograph 7 200819136 Its molecular weight is 899.5~1670.5 thousand auricular. ((1) Optical rotation: [61:] 1) + 115.0. ((: =0.4433,水>(e) Intrinsic viscosity: [々]=〇〇3971~〇〇6255 公合/克. (f) Specific heat Cp . 〇. 2536~0. 3995 卡/克·.c.(g The red ray line spectrum: as shown in Figure 5. (8) Hydrogen nuclear magnetic resonance spectroscopy · · Figure 6. (i) Carbon nuclear magnetic resonance spectroscopy: as shown in Figure 7. (D gas chromatography mass spectrometry analysis As shown in Figure 8. In a preferred embodiment, the molecular weight of the polysaccharide is analyzed by high-performance liquid chromatography. The result is 1092. 25~1477. 75 thousand auricular swallows, the result of the face-to-face implementation is 1285 thousand耳 吞, endogenous viscosity 〇. 0417 dl / gram, specific heat is 〇. 2663 cal / gram · c. • Read _ infrared spectrum results * its composition contains galactose, glucose, fucose, mannose and Aminogalactose. Hydrogen nuclear NMR spectroscopy shows that the biliary body is composed of D-galactose, D_glucose, L_fucose and D_mannose, and these The ratio of sugar is i : 〇.46 : 0.035 : Q. (10): These biliary ostriches have a structural backbone consisting of: (4) endpoints: residues in the middle of fucose or glucose (6): 1,3 - Bonding glucose, ^ 4-bonding glucose, ^6_bonding or u & bonding galactose' and galactose forming a branch at 2-0. These multi-biliary bodies have money on the backbone of the structure. The miscellaneous impurities contained in this judgment were obtained from the water extract of Ganoderma lucidum. The therapeutic effect of the recording is 0.15" / per patient weight. For better implementation, the therapeutic efficacies are HU mg/kg of body weight per patient. In the better practice, the mg/per ounce of “body weight is more than enough to have therapeutic effect. In the best example, 〇.丨~丨•家充/母公斤 patient weight 8 200819136 t '录魏有The treatment agent can be used to combat _ poisoning or damage to the liver. The composition of the present invention comprises _ a pharmaceutical carrier. In a preferred embodiment, the slimming body is a liquid. Or semi-solid, suitable for injection. Suitable pharmaceutical carriers are polar solvents such as water, alcohol, ketones, vinegar and mixtures of the above solvents, mixtures of water and sighs. & Carrier 疋 疋 、 、 、 生理 生理 生理 生理 生理 、 、 、 、 、 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 The present invention also provides a composition containing an anthraquinone neutral multi-enzyme which can be used as an oral medicament, and its characteristics are as follows: (4) Appearance: a colorless and non-fixed shape powder. (6) S夂 test value· (c) Molecular weight: as shown in Figure 4. The molecular weight of 899.5~1670.5 thousand auricular was analyzed by high-performance liquid chromatography. ((1) Optical rotation: 4] 1) + 115 〇 (c =0· 4433, water). (e) Essence Viscosity: ["] = 〇 · 〇 3971 ~ 〇 · 〇 6255 com / g. (1) Specific heat Cp: 〇 · 2536~0· 3995 cards / gram ·. C. (g) Infrared spectrum: as shown in Figure 5. (h) Hydrogen nuclear magnetic resonance spectrum: as shown in Figure 6. (丨) Carbon nuclear magnetic resonance spectroscopy·· As shown in Figure 7. (j) Gas chromatography mass spectrometry: as shown in Figure 8. In a preferred embodiment, the molecular weight of the polysaccharide is determined by high-performance liquid chromatography to be 1092·25~1477·75 thousand amps, and the result of the more preferred embodiment is 1285 thousand amps. Bio-viscosity 〇 · 0417 dl / gram, specific heat is 〇 · 2663 calories / gram ·. C. 9 200819136 It has a therapeutic effect ❹ _ «It is a sputum gram / each sputum collapse weight. In a preferred embodiment, the therapeutically effective front _# is Q.mg/kg body weight per kilogram. In a more preferred embodiment, the G.(4) g/kg body melanose body can have therapeutic efficacy. The best real towel, (UU grams / per metric - human body weight multi-domain body can have therapeutic effects. These π 份 份 对抗 对抗 _ _ _ 或是 或是 或是 或是 或是 或是 或是 或是 或是 或是 。 。 。 。 。 。 。 。 。 。 。 。 。 。 The step comprises - in the embodiment of the touch, the carrier is solid, liquid or semi-solid and suitable for oral administration. Suitable pharmaceutical carriers are polar solvents such as water, alcohol, hydrazine, vinegar and The age of the above solvent is preferably water-repellent or hydrated. In a preferred embodiment, the carrier is suitable for α, water, physiological saline, aqueous buffer solution, physiological saline solution, etc. Health can also be returned to the home, Portuguese, and the temple powder plus sodium stearate. The liquid carrier for oral use can contain water, soybean oil, wine, juice, etc. [Embodiment] The following examples are merely representative of the invention. The special kind of money, but does not mean that the present invention is only limited to this type of brown. "Example 1" (Α) material is the early drop of the cow (also known as α Mycelia) is from Taiwan's "good stem creatures" 200819136 Technology shares limited Provided by Simpson Biotech Co. Ltd. Taiwan. The standard molecular weight marker of pullulan (Shodex Standard P-82) is commercially available from Showa Denko Co. Ltd., Japan. (B) General experimental procedure The optical rotation is measured by dissolving the sample in water and measuring with a JASCO DIP-360 polarimeter. The ultraviolet absorption spectrum is measured and recorded by SHIMADZU UV_2200 UV-VIS. The infrared spectrum is JASCO FT/ The IR-230 infrared spectrometer was recorded with potassium bromide or a liquid photosensitive sheet. The nuclear magnetic resonance spectrum was obtained by Varian Unity plus 500 (H is 500 kHz, C is 125 kHz) and Varian GEMINI 300 (H is 300 kHz, C is 75 kHz. The measurement of the cerium oxide (D20) solution of the polysaccharide is a reference value of 1,4-diozane as an external reference. The gas chromatography mass spectrometer analysis is performed with the SHIMADZU GC-17A gas chromatograph. Sakamoto Electronics Co., Ltd. (JEOL) mass spectrometer. Film color layer analysis is 60" 254 plates (Merck|\/^1^1<& 〇〇, 0.25 mm) or fibers pre-coated with cerium oxide. Plate (Merck Merck & Co, 0.1 mm) completed on the separation The substance is detected by heating to 1 °C with 1% sulfuric acid or multi-layer analysis (ΑΗΡ). The carbohydrate is determined by the sulfuric acid method. "Example 2: Prepared by Antrodia camphorata (AC) (A) Extraction of polysaccharides from Antrodia camphorata and separation of low-temperature dried Antrodia camphorata powder (1.5 kg) were extracted at room temperature for 1 day (41?11 200819136 times), then filtered and dried. The dried residue was immersed in water, allowed to stand at room temperature for 1 hour, and extracted with cesium (8) ° C for 2 hours (3 times). After hot water extraction, the volume of the extract was concentrated to 800 ml, and then 32 ml of alcohol was added. The mixture was thoroughly stirred and placed in the refrigerator for one night. The precipitate was filtered off and rinsed with iced alcohol and then dried. After precipitation with 10 〇/〇 tri-gas acetic acid (TCA), centrifugation (3000 rpm, 10 minutes) was carried out and the fraction dissolved in TCA was collected, followed by dialysis against distilled water for 3 days. The portion which did not undergo dialysis was lyophilized to obtain a brownish residue (AC) crude polysaccharide. Yield: 14.25 g. (B) The above brown residue: AQ crude polysaccharide (1 mg) was dissolved in water by ion exchange column chromatography, and then poured into a DE-52 column (Whatman International Ltd., England 2.0: 20) In centimeters). The column was flushed with 60 ml of water, followed by 60 ml of 〇·5 mol of sodium chloride solution, 60 ml of 1 mol of sodium hydride, 60 ml of 2 mol of sodium chloride, and collected once every 2 ml. . Concentration of water (ACN) and lyophilization yielded 68.3 mg. (CMC7V colloidal filtration JC7V (68.3 mg) was dissolved in 0.2 mol of sodium chloride solution and poured on a T〇y〇pearl HW-65 column (Tosoh, Tokyo, Japan 2.0 h 90 cm), the column was the same The solution was extracted and collected once every 5 ml. The extract was divided into two groups (ACN1 and ACN2) based on the data at 480 nm. Yield: ACN1, 19 mg; ACN2, 49 The ACN2 was further purified by HW-65 column and the same conditions as above to obtain a colorless polysaccharide (referred to as ACN2a, yield 41 mg). 12 200819136 The fraction of the underdosed burdock was separated by the procedure. The undialyzed part (A.) The secreted part of the secreted TCA has a liver-protecting effect. In addition, the Teng-sulfuric acid method is used to react, and the transferred part also contains polyacetate. As shown in the figure, it is separated by DE 52 fiber ion exchange column chromatography. The most potential water solution (CAN) is separated by colloidal transition (Fig. 2). The second liquid separation A· with HW-65 colloid The filter column is further purified to produce a colorless polysaccharide (ACN2a) hepatoprotective component (Figure 3). The above-mentioned Antrodia camphorata (I·5 kg) hot water extraction concentrate 8〇0 is the case of the lion ml in the house, followed by 4 times alcohol precipitation, brain TCA flushing, steamed water dialysis, DE-52 ion exchange tube column Analyze, HW_65 transition and other steps to illustrate the preparation of ACN2a extraction abortion 〇 13 200819136 crude extract (300 ml) 4 times the volume of alcohol

Insoluble Dispensing (20g) 10% TCA Dissolved Dissolved Dissolved Dissolved Dissolved Dissolved Separate Dialysis and Dry AC (Actained 4.75g AC Crude Polysaccharide) Take 3g AC Crude Polysaccharide as Anion Exchange Resin DE- 52 column for gasification of sodium) (water '0.5, 1,2 水分 water (ACN) (2.05 g) 0.5 mol gas sodium (ACA) (0.83 g) colloidal filtration chromatography HW-65 ( Water) (1·7Χ90 cm) CAN1 (0.57 g) ACN2 (1.47 g) Colloidal Filtration Chromatography HW-65 (water) ACN2a (1.23 g) Flowchart 1. Extraction procedure "Example 3: Anthraquinone neutral polysaccharide Structural Analysis" (A) Molecular Weight Estimation The average molecular weight of the polysaccharide (ACN2a) was analyzed by high performance liquid chromatography. The sample was added to a TSK-GMPWXL colloidal filter column (7.8 X 300 mm in-tube diameter, T〇s〇h(10), Tokyo , Japan) and 0.25 ml/min with 0.2 mol of sodium chloride. The purchased Proud 200819136 blue polysaccharide (Shodex standard P-82) was used as the standard molecular weight marker. This polysaccharide (ACN2a) has been used high. The efficiency liquid chromatography proved to be a single liquid separation (Fig. 4), and the molecular weight was estimated to be 1285320 by high-performance liquid chromatography. The polysaccharide was colorless and Powder without fixed shape '[1]0+115.0. (〇= 0.4433, water); endogenous viscosity [11]=〇〇417 com/g (Oswald viscometer, Ostwald viscometer), specific heat Cp: 0.2663 calories per gram C (measured by DSC method (thermal differential scanning analyzer)) containing 〇·2〇% protein (measured by the Bradford method) and 0.12% nitrogen (measured by Bradford analysis method) in ACN2a; Sulfur did not appear in ACN2a (measured by the Barium rhodizonate method). (B) Identification of sugar components Polysaccharide (2 mg) was dissolved in 2 ml of 2N trifluoroacetic acid (TFA) and then sealed. Vapor at 125 ° C. The pressure steel was hydrolyzed for 1 hour, and the mixture was removed by evaporation to remove the TFA. The hydrolyzate was reduced with sodium borohydride. The triterpene group was prepared by the silblender_HTP method and used as a gas chromatography mass spectrometry (column model: DB], J&W Scientific, 0.25 mm id· X 30 m; column temperature: 50 ° C ~ 190 ° C, 5 ° C / min; then 190 ° C, 12 min; Helium carrier flow 4 · 25 kg force /cm). Purified according to the identification of sugar components (Figure 5) The polysaccharide contains galactose, glucose, fucose, mannose and galactose (1: 0.46: 0.035: 0.016 · · 0.092), and about 62.38 % of the quinone is galactose. The ACN2a optical rotation is +115.0. (c = 0.4433, water). This result indicates that the constituent sugars may have an a-D configuration or a β-L-configuration (determined by Hudson's law of the same rotation). According to the results of the determination of the absolute configuration of the constituent sugars (Fig. 6), the absolute configurations of the constituent sugars are L-fucose, D-galactose, D-glucose and D-mannose, respectively. (C) Determination of the absolute configuration of the constituent sugars The absolute configuration of the constituent sugars was determined according to the method reported by Hara et al. The polysaccharide (1 mg) was steam sterilized at 2 N TFA at 125 ° C for 1 hour, and the TFA was removed by evaporation while the mixture was dried to obtain a sugar fraction. The sugar solution (2 mg) was mixed with L-cysteine methyl ester hydrochloride (3 mg) and heated to a bite solution (0.5 mL: liter). 6 (TC, 1.5 hours, then dried with nitrogen. The dried sample was trimethylsulfonated with silblender-HTP (0.4 mL) at 60 ° C for 1 hour. After 3 ml of gas and 3 ml of water, The chloroform layer extract was analyzed by gas chromatography mass spectrometry (column type: DB-wax, J & W Scientific, 30 m X 〇 · 25 Φ m, column temperature: 50 ° c ~ 230 ° c, 10 ° c / min, then rise to 23 (TC, 12 min, Helium carrier flow 4.25 kgf / cm) (D) Methylation analysis 5 house grams of polysteroids according to Anumula and Taylor's method with hard nail methylation methylation. The grafted polysaccharide was hydrolyzed with 4 (tetra) TFA under 125 〇 vapor pressure steel for 9 Torr. After removal by vapor, the hydrolyzed product was converted with ammonium hydroxide containing 3 mg/ml sodium hydride. The sugar alcohol is then acetylated. Part of the methylated sugar alcohol oxime is analyzed by gas chromatography and analyzed by f-spectrum (Record: Sp_233G, Su P—,

Bellefnte 'Pa•' 6Q mχ 025 mm, (four) micron thick thin layer, 氦 as carrier gas 16 200819136 body 'column temperature from 160 ° C at 2 ° C per minute to 210 ° C, then From 210 C to 5 ° C / min to 240 ° C, stay at 240 ° C, 14 minutes), the area under the peak is corrected with the published molar response coefficient. The derivatized compound is compared with the infrared relative residence time of L5-dioxyacetic acid 1_2,3,4,6-tetramethoxynonylglucitol, and is detected by infrared gas chromatography electron impact ionization mass spectrometry. Split mode. Fourier transform infrared spectrometer, as shown in Figure 6, has three absorptions at 115丄22 cm _ ^1079.94 cm -1 and 1033.66 cm -1, indicating that the sugar may appear linear (the form of the five-ring sugar will only be present in this area). Two sections absorb light). Since the absorption spectrum appears at 917.95 cm-1, there may be the presence of D-ring glucose. In addition, the absorption light at 873.6 is peculiar to mannose and galactose. Observing the 16367.27 cm _ ing 2 base absorption spectrum and finding that there may be the presence of amino sugars. The conclusions of these analyses are the same as the initial analysis. In the hydrogen nuclear magnetic resonance spectrum (Fig. 7), the hydrogen signal is more than 4·8 parts per million (4·885, 4 and 4·% 3 parts per million), indicating that the constituent sugar has an α configuration. This conclusion has the same result as the analysis of the optical rotation. In addition, the position of less than 4·8 parts per million (4·738, 4·663 parts per million) is still in the scale of the gas atomic position. The signal for methylation protons was observed at L134 parts per million due to the thiol group of the fucose residue. The signal for the ectopic is observed at less than Μ parts per million, like a single line. These results indicate that the fucose residue has a p_L _ configuration. (a_L_fucose ectopic signal is detected at greater than 5.0 parts per million). 17 200819136 In the carbon nuclear magnetic resonance spectroscopy (Fig. 8), the signals of C-4 and C-5 were observed at less than 8 〇 parts per million, indicating that the constituent sugars are in the form of hexacyclic sugars (five The chemical shifts of the cyclic sugars c_4 and C-5 occur in the region of 8 〇 to 85 parts per million), which is the same as the infrared analysis. In addition, the signal of the methyl group was observed at a rate of 13.7 parts per million and was determined to be a methyl group of a fucose residue. These results show that the fucose residue is £_fucose (the C-6 signal of d-fucose is present in the 60 to 65 parts per million region). This is the same as the conclusion of the nuclear magnetic resonance spectroscopy. The results of the methylation analysis have been compiled in Table 1. These results show that the structure of ACN2a is fucose at the end point, 1,4-bonded glucose, 1-6-linked galactose, and ι, 2,6-bonded The galactose residue consists of a small portion of the terminal endogenous glucose and a u-bonded glucose residue. By methylation analysis, ACN2a contains a skeleton formed by ct_D _1,6-galactose (α·D -1,6_ and aD -1,2,6_) galactose, which accounts for about 72.82 %. The number of branched sugars is approximately 15.75% of all residues, and these branches are the positions (2-0) of oxygen on the second carbon of galactose attached to the backbone. 200819136 Table 1: Results of methylation of ACN2a. Methylated sugar Mo Er Tr mass-to-charge ratio (M/Z) Bonding type ratio 2,3,4-trimethyl-fructose 0.209 0.789 71,89,101,117 ,131,161,175 fructose-(1 + 2,3,4,6-tetramethyl-glucose 0.084 1 71,87,101,117,129,145,161,205 glucose-(1 + 2,4,6-trimethyl-glucose 0.026 1.31 71,87,101,117,129,161,233 ">3)-Glucose-(1·> 2,3,6-trimethyl-glucose 0.157 1.489 87,99,101,113,117,233 +4)-glucose-(1; 2,3,4-trimethyl-glucose 1 1.6 71,87,99,101,117,129,161,189 +6)-glucose-(1 + 3,4·trimethyl-galactose 0.276 1.881 87,99,129,189 +2,6)-glucose_(1"> TR is the relative time of various ingredients, Relative to 1, 5-0-2, 3, 4, 6-tetradecyl-glucose "Example 4: Neutral polysaccharides (ACN2a) against Λό/;/0ιιι^ι^/7·ιι#ιι acwes_LPS induction The protective effect of liver toxicity" (A) Provision of jR slave 71 and other solutions

Prap/wi/kic, such as 7·ϋ#ιι tfcnes (acnes only) (ATCC 6919) and brain heart extract culture medium (Wako Pure Chemical Industries, Ltd. Osaka, Japan), supplemented with L-cysteamine Acid (0.03%) and Tween 80 (0.03%) were cultured for 48 hours at 37 °C under anaerobic conditions. The culture was centrifuged at 5500 xg for 15 minutes at 4 °C and phosphate-physiological saline buffer (PBS). ) Cleaning. The centrifuged bacterial pellet was suspended in 300 ml of PBS' and heated at 80 C for 30 minutes to kill the cells, and then; dried to prepare the beta (7) from the powder. LPS from Escherichia coli cW 055: Β 5) was purchased from Sigma-Aldrich, 200819136^ (Steinheim, 〇ermany). FK506 (tacrolimus hydrate, an immunosuppressive agent) was supplied by Fujisawa Pharmaceutical Co., Ltd. (Osaka, Japan). (B) Male ICR mice (18 to 20 g weight), male BALB/C (18 to 20 weight), and male Wistar rats (106-180 g weight) (SLC, Hamamatu, Japan) It is a liver-protecting experiment for hepatotoxicity induced by R acnes-LPS. These animals have been adapted for one week before the experiment. (C) Liver protection test (oral) Liver protection activity was studied using the following experimental design: A: ICR mice (1) general control group (without any treatment); (2) household (four) Lie-Lps ( 〇·15mg-〇·〇5μg/small gas) treatment control group; [(3)〇2g/kg/day, (4)0.4g/kg/day, (5) 0.8g/kg body weight/day, Oral] plus ρ Gu Lie - LPS treatment; (6) FK506 (1 mg / kg body weight, oral) plus LPS treatment. B· BALB/C small machine (1) general control group (without any treatment); (2) household-LPS (0.20 mg_〇·075 μg/mouse) treated control group; (3) (0.4 g/ Kg body weight/day, oral (4) (〇·4 g/kg body weight/day, orally) plus P ac72a-LPS treatment C: Wistar rats (1) general control group (without any treatment); (2) a Control group treated with ac__Lps (5 mg-5 μg/rat); (3) dCAr2a (〇·2 g/kg body weight 20 200819136 / day, orally) plus cadaver/^ — LPS treatment. HU 1 Π i 7 1 hour 6 hours 1 卜i Bu intravenous injection p. (0·15 亳g/mouse) Collect blood samples intravenous LPS 0.05 μg/mouse) Flowchart 2·Animal experiment (oral > 1 hour) The axe, which was killed by heat, was suspended in physiological saline and injected into the animal from the tail vein (Flowchart 2). Seven days later, the group injected with intravenous LPS produced severe hepatocyte injury. JC7V2a was given daily. Oral administration, allowing animals to be absorbed through the digestive tract for 7 consecutive days, LPS was injected one hour after JC7V2a was given on the eighth day. Groups using FK506 were used as positive pairs. According to the group, and also the animals were absorbed through the digestive tract, the time was applied at 48, 36, 24, 12 and 1 hour before the intravenous injection of LPS. After 6 hours after the injection of Lps for 6 hours after injection of Lps, blood samples were collected. The test was performed in a test tube for liver damage and sacrificed. The blood samples were centrifuged at 35 〇〇Xg for 15 minutes and serum was collected for use as a sample. All samples were stored at 2 〇〇c. It is not resolved until it is analyzed. Serum towel scorpion sulphate transaminase S| (AST) and propyl lining (ALT) activity are hepatocellular secrets, the secret of these two squamos c I Test Wako) (Wako Pure Chemical Industries, Ltd. Osaka, Japan) 〇21 200819136 (D) After hepatocyte morphology sacrificed mice, the field was collected and the fresh liver was quickly removed, cut into pieces, and immediately 10% of the buffered Marlin buffer solution, and then dehydrated with 5 (M_ alcohol solution, then buried in stone, 4~5 mm cut-slice, shattered by hematoxylin-eosin staining. (E) The liver protection effect of the liver protection experiment (intravenous injection) is the following Designed to study: A: ICR mice (1) general control group (without any treatment); (2) h__Lps (0.15 mg - 0.05 μg / mouse) treated control group 四 (4) 〇 2 g / kg / day, (4) 0 .4 g / kg / day, (5) 〇. 8 g / male (four) weight / day, intravenous injection + plus /> a · -LPS treatment; (6) FK5 〇 6 (four) g / kg body weight intravenously) On acwey-LPS processing. B: (1) general control group (without any treatment); (2) ρ α_—— (〇2〇 mg 0.075 μg/mouse) treated control group; [(3) 〇 · 4 g / kg body weight / day, (4) 〇·8g/kg body weight/day, intravenous injection] plus households treated with ~Lps. The mode of acute liver injury (sudden hepatitis) experiment can be induced by the method described above. The method of intravenous injection is the same as the method of oral administration. The method of intravenous injection B is on the eighth day, injection and dissolution from the tail vein. Lps in physiological saline once (flow chart 3). Gold liquid samples were collected into test tubes as a sample buckle for analyzing liver damage after 6 hours of LPS injection, and then sacrificed. The tubes were centrifuged at 3500 xg for 15 minutes and gold was collected. 22 200819136 Serum was used as a sample for use. All samples are stored at (9)t and are not used until analysis. The activity of aspartate transaminase (AST) and alanine transaminase (10) in serum is an indicator of hepatocyte injury.

A

Intravenous corpse (〇·15 mg/mouse) Intravenous sputum LPS Collect blood samples (0.05 μg/mouse) Do not experiment (intravenous) 鋥砉(F) This green treatment of heart Lps-induced liver injury in mice Effect The effect of JC7V2a treatment was studied using the following experimental design: (1) general control group (without any treatment); (2) control group treated with household-LPS (0.15 mg 〇·〇5 μg/mouse); [(3) 〇·4 g/kg body weight/day, (4) 〇·8 g 7 kg body weight/day, (5) h6 g/kg body weight/day, (6) 3.4 mg/kg body weight/day, (7) 6.4 耄G / kg body weight / day, intravenous injection] plus household a_-LPS treatment; WFK5 〇 6 (1 gram / a kg body weight, oral) plus Huo Li _ lps treatment. The mode of acute liver injury (sudden hepatitis) experiments can be induced by the methods described above. 23 200819136 Three hours after the injection of LPS on the eighth day, the tail was intravenously injected in physiological saline (flow chart 4). Blood samples were collected 3 hours after the injection of LPS as a group treated with only one P-LPS treatment, and other blood samples were collected into test tubes as samples for analyzing liver damage after 6 hours of LPS injection, and then sacrificed. The tubes were centrifuged at 3500 xg for 15 minutes and serum was collected as a sample for use. All samples are stored at _1 2 ° ° C until the analysis is needed; East use. Serum aspartate transaminase (10) and alanine transaminase (ALT) activity are indicators of hepatocyte injury, and the activity of these two lucifers can be determined by the kit. Intravenous injection of ACN2a 7 days 3 hours 3 hours 4 intravenous corpse aeries (〇·15 mg/mouse) ^ i Collection C ^ 1 h : on3 blood phase 丨 under-concentration blood sample 1 intravenous LPS (〇·〇 5 g / mouse) After injection of LPS, reinjection is a method to effectively produce experimental acute liver injury (sudden hepatitis). Animals in large neighbors die within 24 hours of injection of LPS. Figure (treatment effect, time 2 (G) results a · evaluation of experimental animals 200819136 severe liver damage, in this study, we found that "the best amount of kiss must be (〇i5 mg -0.05 μg / mouse) All animals are still in a state of survival in the case of acute liver injury (sudden hepatitis) and the most severe steel point of liver injury is 12 hours after intravenous injection of Lps (Fig. 10 and _), so in this invention The towel samples were collected after 6 hours of LPS injection. b. The hepatoprotective effect exhibited by oral ACN2a Table 2 shows that AC^a is necessary for the treatment of serum mice and AST live (4). Serious « Toxic Wei in the _ H 〇. 8 g / kg kg of ACN2a pre-discriminatory The object is obviously inhibited (4) Qing, so Yan & can protect the liver cells of mice against the α τ ρς (7) (2)-LPS-induced liver toxicity, in addition, this health care effect is the gamma of ACN2a «. in Caixian Treatment of 0.4 g / kg kg of agricultural __ mice, or in all pre-treated 克 25 g / kg kg of dC7Va2 Wistar large window Φ, 磬 n dry serious liver toxicity reaction is also significantly reduced (four). 5), showing that the liver damage (surgical hepatitis) recommended by the _-LPS material in the murine animal has a protective effect. Table 2. ACN2a induces liver damage to the household and wLPS. Effects of ALT and AST activity in Jinqing _ group dosage (g/eight--~--AST(IU/L) ALT (IU/L) ICR mouse control group' 397.3 ± 201.35** 49.2 soil 18.64** ( 10 mice per group) - LPS ° · 15 mg gamma / qi 2196.4 ± 491.39 1762.2 ± 605.33 25 200819136 P. acnes - LPS + ACN2a 0.2 2046.4 ± 776.37 1704.6 Soil 521.72 P. acnes - LPS + ACN2a 0.4 1748.3 ± 439.79 * 1156.55 ±517.09* P. acnes - LPS+JC steals 0.8 1392.1 ±460.36* 94 8.6 ±555.30* P. acnes — LPS+FK506 1 mg/kg 1236.0 ±625.00** 685.0 ±344.28* Control group / 195.4 ± 56.56* 38.9 ± 6.63** BALB/c mouse P. acnes - LPS 0.2 mg-0.075 Micrograms/mouse 519.0 ± 128.25 208.0 ±41.30 (8 mice per group) P. acnes - LPS+dC steals 0.4 1 - 431.9 ± 119.71* 129.0 ±45.28* Control group / 104.2 ±70.54** / 65.4 ± 9.02* Wistar rat P. acnes - LPS 〇·5 mg-5 μg/rat 1095.7 ±240.34 975.3 ± 644.97 (8 rats per group) P. acnes - LPS+ACN2a 0.2 439.5 ±253.26* 306.0 ±130.76* Experimental results It is based on the average value plus or minus one standard deviation table and the mother's 'quasi-difference from the experimental results of each group of 10 or 8 experimental animals. Compared with the control group injected with h-LPS, * represents a small P value of <0.05 by the t-test; and ** represents a P value of &lt; 0.0 (H. The effect of ACN2a on liver protection is observed by _study Make a re-determination (Figure 12). Injection h-LPS causes granuloma, recorded necrosis and ICR mouse liver fine 26 200819136 Cell immersion wave 1. #♦县处理' brain shout less cell infiltration damage score and inhibit liver Cell necrosis (Table 3). Expanded hepatocytes are much larger than normal normal hepatocytes. Table 3. Hepatic tissue damage scores in different doses Animals scores granuloma hemorrhagic necrotic cells infiltration control group

P. acnes - LPS 2 1 0 0 3 3 2 2 ICR mouse corpse - LPS~MCTV2a (0.2 g/kg) ^ (10 mice per group) corpus ac72a - LPSWCA^(〇.4 g/kg) 4 corpse (10)^-LPS+dC7V2a (0.8 g/kg) 3 corpse-LPS+FK506 (1.0 mg/kg) 2 Light microscopy statistics of liver tissue damage score: 〇 = no visible cell damage; 1 = less tissue Pointed hepatocyte injury in the dish; 2 = 25-5 hematocytic damage in the tissue; 3 = large area cell damage; 4 = overall hepatocyte necrosis. C. Liver protection effect of intravenous ACN2a Figure 12 shows the effect of ACN2a on ALT activity in serum of ICR mice treated with p-sink _LpS. In all animals that were pre-injected with 0. 2 or 〇 4 mg/kg kg of ACN2a in the tail, the severe hepatotoxic response was significantly inhibited as the amount increased (ρ&lt;〇·〇5), Therefore, intravenous injection of ACN2a can protect the liver cells of mice against the ac«a_LPS-induced liver toxicity. 27 200819136 The liver protection effect of intravenous ACN2a was determined again by histological observation (Fig. 14). Injection of p acnes — LPS caused cell swelling, hemorrhagic necrosis, and hepatocyte infiltration in ICR mice. However, pretreatment reduced the cell infiltration injury score and inhibited hepatocyte necrosis (Table 4). Table 4. Different doses of liver tissue damage scores Animal group injury scores granuloma hemorrhagic necrotic cells infiltration control group 0 0 0 R aeries ~ LPS 3 4 3 ICR mice P. acnes - l^^ + ACN2a (0.2 mg /kg) 0 0 1 (10 mice per group) P. acnes - LPS+^CA/2a (0.4 g/kg) Intravenous 0 0 1 R acnes - hVS^-ACN2a (0.8 mg/kg) 0 0 2 Optical microscopy statistics of liver tissue damage scores: 〇 = no cell damage visible to the naked eye; 1 = less than 25% of punctate hepatocyte damage in the tissue; 2 = 25-50% hepatocyte damage in the tissue; 3 = large area of hepatocyte injury; 4 = overall hepatocyte necrosis. In addition, hepatocytes in the proliferative phase were also found in tissue observation (Fig. 15). Pre-treatment of dC7V2a mice promoted hepatocyte proliferation and improved damaged hepatocyte repair function. 28 200819136 d. Comparison of the effects of oral and intravenous ACN2a on liver protection The effects of two different modes of administration on serum ALT activity in ICR mice treated with ρ β(7)-Lps were compared (Figure 16). In all pre-administered oral 〇2, 〇·4 or 〇·8 g/kg kg, or given intravenous 〇·2 or 〇·4 mg/kg kg of ACN2a into ICR mice, severe liver toxicity was evident Inhibition with increasing dosage (ρ &lt; 0.05) indicates that it is effective to protect ICR mice from hepatic toxicity induced by α__Lps, whether administered orally or intravenously. In addition, although the effect of liver protection obtained by intravenous injection of only 1/1000 ′ of the oral dose is 1 〇〇 more than that of oral administration, intravenous injection is the most preferred method. In summary, oral or intravenous injection of jCAr2a exhibited hepatoprotective effects in murine animals (ICR mice, BALB/c gas, and purchased rats) in liver injury induced by H(7)tetra-LPS. "Example 5: Mechanism of liver protection mode" The experimental mode mechanism induced by the household sinking T2 〇 LPS is shown in the flow chart 5. Injection of corpse into the tail vein of the mouse, resulting in monocular leukocyte infiltration of the liver, so the liver's giant sputum cells increased 'and later intravenous injection of a small amount of Lps will activate (4) giant gamma cells, which then began to secrete cytokines Such as tumor necrosis factor (TNF), IL_1, soluble il_2 artifacts. These two cytokine damage liver cell lines necrosis of liver cells by the following three methods: 29 200819136 and by platelet activating factor (PAF) and leukotriene. 2) The doctor and U necrosis of the liver cells by a large area of the nucleus and the micro-pathway. In this mechanism, oxygen free radicals play a major role. 3) Because combined with the soluble positive _2 receptor, the number of _2 is reduced, resulting in a decrease in inhibitory tau cells, an increase in killer tau cells (cyt〇t〇xic τ cdl, CTL), and killer T cells will Liver cells are necrotic on a large area. The natural production of O. chinensis polysaccharides to scavenge oxygen free radicals is effective and can increase IL-2. In this fortune, it was found that natural polysaccharides and neutral polysaccharides (A(:N2a) can protect mouse liver cells against the effects of liver-LPS-induced liver toxicity. It is understandable that A. angustifolia polysaccharides can exhibit liver-protecting activity because At least it has the ability to scavenge oxygen free radicals, block the mechanism by which AMA-LPS induces liver toxicity, or increase IL-2 and kill killer T cells, thereby protecting the liver. 200819136 Endotoxemia (P. acnes)

• i fine) macrophage 1 LPS

The present invention has been described in detail, and it is obvious that any person skilled in the art can use it and make various substitutions, modifications or improvements, but obviously, these cannot be combined with the present invention. Separation of spirit and scope of invention. Those skilled in the art will readily appreciate that the present invention can readily achieve the objectives and achieve the results and advantages mentioned, as well as those which are present therein. The herbal composition from which the present invention is produced is representative of the preferred embodiment, which is shown and is not limited to the field of the invention. Those skilled in the art will be aware of the modifications and other uses therein. These modifications are included in the spirit of the present invention and are defined in the scope of the patent application. It is obvious to those skilled in the art that the invention disclosed herein will be susceptible to various substitutions and modifications without departing from the scope and spirit of the invention. All patents and publications mentioned in the book are in the general technical scale of the invention domain. All patents and publications are hereby incorporated by reference, as if each side was specifically and separately indicated in accordance with the reference. The invention exemplified herein may be practiced in the absence of any element, perhaps multiple elements, limitations, or limitations. For example, in each instance of this document, the terms "including", "including at least" and "containing" are used in any of the terms. The nouns and expressions of the household are as a description of _ rather than a limitation, and there is no intention to use such nouns and expressions other than any equivalent: ΓΓ characteristics or parts thereof, but it is necessary to recognize the various changes. It is possible to be within the scope of the special request of the present invention. Therefore, the present invention has been specifically disclosed in accordance with the preferred embodiments and any features, and the kiss heart is still within the scope of the patent application of the invention. Branch b is still in this 32 200819136 [Simple description of the diagram] Figure 1: Analysis of the results of AZ by DE-52 ion exchange column chromatography (detection method: sulfuric acid method) Figure 2: HW-65 Colloidal Filtration Column Chromatography Analysis of Anthraquinone (ACN) Results (Detection Method: Age Sulfuric Acid Method) Figure 3: Analysis of Anthraquinone (ACN2) by HW-65 Colloidal Filtration Column Chromatography (Detection Method: Phenol - Sulfuric acid method) Figure 4: Analysis of Anthraquinone (ACN2-i) by HW_65 colloidal filtration column chromatography (detection method: phenol-sulfuric acid method) Figure 5: Infrared spectrum of ACN2a (detection method: potassium bromide) Figure 6: Hydrogen nuclear magnetic resonance spectroscopy of ACN2a (H_NMR, measured by heavy water) Figure 7: Carbon (13) nuclear magnetic resonance spectrum of ACN2a (Ci3_NMR, measured by heavy water) Figure 8: Analysis of the saccharide content of ACN2a by phase-chromatography mass spectrometry (detection method: phenol-sulfuric acid method) Figure 9: Configuration of saccharide components in ACN2a (L-shaped or D-shaped) Figure 10: Asparagine in serum Aspartate aminotransferase (AST) is a graph of current quantity and time. Nor, control group. Con6, blood samples collected 6 hours after intravenous injection of bacterial toxin LPS. Conl2, a blood sample collected 12 hours after intravenous injection of bacterial toxins. C〇nl8, intravenous injection of bacterial toxins 33 200819136 (four) after 丨M, when received _ blood samples. The result of the experiment is that the fresh mean value is added or subtracted from a standard series, and the drunk-a fresh silk is calculated from the actual fruit of 1Q only. With the P-LPS test peach, * represents the p value obtained by the t-test is small &lt; G G5; and ** represents the p-value &lt; 〇〇 图 图 十 - · serum aspartate transaminase Quantities and time of _ ga, the control group. C_, a blood sample collected 6 hours after intravenous injection of the bacterial toxin. - 12 ' Blood samples collected 12 hours after intravenous injection of bacterial toxin Lps. C〇nl8, a sample of money collected 18 hours after intravenous injection of bacterial toxin Lps. The experimental results are expressed as a mean plus or minus one standard deviation, and each standard deviation is calculated from the experimental results of 10 and returning to the father (ICR). * indicates that the p value obtained by the t-test is small &lt; 〇〇 5 ; and ** represents the P value &lt; 0.01 〇 Figure 12: Oral ACN2a induces mice against pac72^-LPS The impact of liver damage. Nor: Control group. Con: Apply the group of the households to the ^-LPS. FK506: 1 gram/kg of tacrolimus hydrate (FK506, a potent immunosuppressant) and π-listen-LPS. A0.2g, /;· Team: A02a and households with 0. 2g/kg. AtUg, /)· 〇 · · Apply 0.4 g / kg of ACN2a and P. awa-LPS. A0.8g, /;· 〇 : ACN2a and households at 0·8 g/kg Figure 13: Effect of ACN2a on serum transaminase levels in serum of ICR mice induced by ρ βLPS. Nor control group. Con : Only the application of P acn to -LPS 34 200819136 group. A0.2: ACN2a 0.2 mg/kg (administered in equal doses according to body weight) and /&gt;· acna_LPS. A0.4= ACN2a 0·4 mg/kg (administer the same proportion of the drug by intravenous injection) and P ac72^_LPS. Α0.8 = ACN2a 0.8 mg/kg (administered in equal proportions by intravenous injection) and P acT^-LPS. FK506 = FK506 1 mg / kg (by weight, oral administration of the same proportion of the drug) and the body said ^ y-LPS. The experimental results were calculated by subtracting one standard deviation from the mean and each standard deviation from the experimental results of 10 outbred small fvXICRs. Compared with the control group in which acnes-LPS was injected, * represents a small P value obtained by the t-test; 〇·〇5; and ** represents a P value &lt; 0 01. Figure 14. Effect of intravenous ACN2a on P acwa-LPS-induced liver damage in mice. Nor: · Control group. Con : A group to which P(10)7^y-LPS is applied. FK506: Administration of 1 mg/kg FK5〇6 and cadaveric LPS. A0.2g, human ν·: ACN2a and /! awes-LPS applied at 〇·2 克/kg. A0.4g, ί· ν·: Apply 〇·4 mg/kg of ACN2a and /: (iv) his LPS. A0.8g, /· κ: ACN2a and household a__LPS were administered at 8 mg/kg. All of the above are based on body weight, and an equal ratio of the drug and the household ac/^-LPS are administered intravenously. Figure 15: Effect of ACN2a on hepatocytes in hyperplasia. Test = control group. C〇m, the group that applies the household sink-LPS. A0.4 g/kg, ρ· 〇· = ACN2a 0·4 g / kg (oral) and 7^_-〇&gt;8. Eight 0.4 1^ know, ^= eight 0: 0.4 mg/kg (intravenous) and P acwey-LPS. The arrows show the cells that are proliferating. Figure 16: ACma in the serum of ICR mice induced by h__LPS in the serum of Jinqing 35 2〇〇8l9l36

? The effect of V transaminase content. N〇r=Control group. C〇n=Only the group of p ac7^_Lps is applied. A0.2 = ACN2a Oral 0.2 g / kg or intravenous 〇 2 mg / kg ' and P a_-LPS. A0.4=ACN2a Oral 〇.4 g/kg or IV injection 0.4 gram/kg’ and household (10) (10). A〇 8=ACN2a Oral 〇 8 g / kg or intravenous 0.8 mg / kg, and f from ps. FK5〇6: FK506 1 mg/kg (oral) and household (10). Lps. The results of the experiment were expressed as mean plus or minus - standard deviation, and each standard deviation was calculated from the results of a single outbred mouse *). Compared with the control group of APS and APS, the P value represented by the t-test is small &lt;0.05; and ** represents the P value &lt;〇·〇卜 36

Claims (1)

200819136 * ^ Twenty, the scope of application for patents: 1 - A kind of astringent polysaccharide containing astringent _ composition, can be used as an injection, its characteristics are as follows: (a) Appearance: colorless and no fixed shape of the powder, (b Acid value: neutral, (c) Molecular ΐ • The molecular weight of the product was analyzed by high-performance liquid chromatography to be 899·5~167〇·5 thousand deafs, (d) Optical rotation: [a]D +115〇?(c=〇4433, water), (4) Intrinsic viscosity: [η]=0.03971~0.06255 com/g, (7) Specific heat Cp: 0.2536~0.3995 cd/g·. C, (g) Infrared spectrum: as shown in Figure 5, (h) Hydrogen nuclear magnetic resonance spectrum: as shown in Figure 6, (1) Carbon nuclear magnetic resonance spectrum: as shown in Figure 7, and 1 gas chromatography Mass spectrometry: as shown in Figure 8. 2. The composition according to claim 1, wherein the effective dose of the polysaccharide is administered 〇·〇1-5·〇mg/kg body weight. 3. The composition according to claim 2, wherein the effective dose of the polysaccharide is administered 〇·〇5_3·〇mg/kg body weight. 4. The composition according to claim 3, wherein the effective dose of the polysaccharide is 〇·1-2·〇mg/kg body weight. 5. The composition according to claim 4, wherein the effective dose of the polysaccharide is administered 〇·Μ·0 mg/kg body weight. 37. The composition of the invention of claim 1, wherein the molecular weight of the poly-[the molecular weight] is 1092.25~1477.75 thousand deuterium. 7. According to the towel, the composition of the full-length 6 is used, and the molecular weight of the multi-_ is analyzed by a high-performance liquid chromatograph and the result is 1285 thousand auricular. 8. The composition of the patented fine 1 has a tactile viscosity of _17 com/g. 9. The composition according to claim 1, wherein the specific heat of the polysaccharide is 0.2663 calories per gram. C. 10. Root shots of the patented composition of i, which is resistant to liver toxicity or liver damage. • A composition according to the scope of the claimed patent, which further comprises a pharmaceutically acceptable carrier. According to the composition of the composition, the carrier is in a liquid or semi-solid form. A composition of each of the aristolochiatic poly-audio can be used for oral administration, and its characteristics are as follows: (a) Appearance: a colorless and non-fixed shape powder, (b) Acid value: neutral, (c Molecular denier. Li·Molecular chromatographic analysis of its molecular weight of 899.5~1670.5 thousand auricular, (4) optical rotation · · [a] d + 115.0? (c = 0.4433, water), (e) Ben ^ tempo soil &amp; bena: [η] = 0 03971 ~ 0 · 06255 com / g, (f) specific heat CP: 0.2536~0.3995 card / gram ·. C, (g), 〇: external spectrum ·· As shown in Figure 5, 38 200819136 (9) Hydrogen nuclear magnetic resonance spectroscopy · · As shown in Figure 6, (0 stone anti-atomic non-resonant light: as shown in Figure 7 And (1) gas chromatography mass spectrometry: as shown in Fig. 8. 14. Composition according to patent application No. 13 0. 01-5·0 g/kg body weight 15. Composition according to patent application No. 14 - 3.0 g / kg body weight. 16. The composition according to claim 15 of the composition of 0.1 - 2.0 g / kg body weight. 17. The composition according to claim 16 of the composition 0.1 - 1.0 g / kg body weight. The effective dose is administered 'where the effective dose of the polysaccharide is administered' wherein the effective dose of the polysaccharide is administered 'where the effective dose of the polysaccharide is administered. 18. The composition according to claim 13 wherein the multifaceted The molecular weight of the polymer was analyzed by high-performance liquid chromatography to be 1092.25~1477.75 kTorr. 19. According to the composition of the cap patent range IS, the molecular weight of the towel polysaccharide was analyzed by high-performance liquid chromatography. It is 1285 thousand deaf. 20.According to The composition of the invention of claim 13 wherein the endogenous viscosity of the polyp is 〇〇417 com/g. 21. The composition according to claim 13 wherein the specific heat of the polysaccharide is 〇·2663 cal/g·C. 22. The composition according to claim 13 which is resistant to hepatotoxicity or liver damage. 23. The composition according to claim 13 further comprising a pharmaceutically acceptable carrier. 39 200819136 24. According to the scope of application A composition of 23 wherein the carrier is in the form of a solid 'liquid or semi-solid.